Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754820 | G7JXH1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 6.02e-32 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0043980 | Msa0754820 | 0.812520 | 4.068629e-51 | -8.615850e-47 |
| Msa0071250 | Msa0754820 | 0.833777 | 4.388880e-56 | -8.615850e-47 |
| Msa0104800 | Msa0754820 | 0.822925 | 1.825023e-53 | -8.615850e-47 |
| Msa0610180 | Msa0754820 | 0.801174 | 1.019610e-48 | -8.615850e-47 |
| Msa0679030 | Msa0754820 | 0.801352 | 9.374867e-49 | -8.615850e-47 |
| Msa0705990 | Msa0754820 | 0.800885 | 1.168330e-48 | -8.615850e-47 |
| Msa0721280 | Msa0754820 | 0.800895 | 1.162906e-48 | -8.615850e-47 |
| Msa0738030 | Msa0754820 | 0.809099 | 2.236974e-50 | -8.615850e-47 |
| Msa0754820 | Msa0796620 | 0.850487 | 1.644722e-60 | -8.615850e-47 |
| Msa0754820 | Msa0817460 | 0.800785 | 1.224619e-48 | -8.615850e-47 |
| Msa0754820 | Msa0833900 | 0.879775 | 9.462377e-70 | -8.615850e-47 |
| Msa0754820 | Msa0834170 | 0.845078 | 5.081919e-59 | -8.615850e-47 |
| Msa0754820 | Msa0865030 | 0.803646 | 3.156976e-49 | -8.615850e-47 |
| Msa0754820 | Msa0890550 | 0.803813 | 2.914656e-49 | -8.615850e-47 |
| Msa0754820 | Msa0894710 | 0.821512 | 3.881567e-53 | -8.615850e-47 |
| Msa0754820 | Msa0934140 | 0.803083 | 4.128200e-49 | -8.615850e-47 |
| Msa0754820 | Msa0936390 | 0.807468 | 4.980890e-50 | -8.615850e-47 |
| Msa0754820 | Msa0980880 | 0.826873 | 2.135578e-54 | -8.615850e-47 |
| Msa0754820 | Msa0994370 | 0.826054 | 3.348808e-54 | -8.615850e-47 |
| Msa0754820 | Msa1063420 | 0.801960 | 7.034462e-49 | -8.615850e-47 |
| Msa0754820 | Msa1207310 | 0.805120 | 1.555807e-49 | -8.615850e-47 |
| Msa0754820 | Msa1294820 | 0.811098 | 8.298748e-51 | -8.615850e-47 |
| Msa0754820 | Msa1307180 | 0.825578 | 4.343330e-54 | -8.615850e-47 |
| Msa0754820 | Msa1316360 | 0.827910 | 1.204798e-54 | -8.615850e-47 |
| Msa0754820 | Msa1332050 | 0.803691 | 3.089873e-49 | -8.615850e-47 |
| Msa0754820 | Msa1346840 | 0.801960 | 7.034468e-49 | -8.615850e-47 |
| Msa0754820 | Msa1357890 | 0.802425 | 5.644515e-49 | -8.615850e-47 |
| Msa0754820 | Msa1359400 | 0.813876 | 2.049654e-51 | -8.615850e-47 |
| Msa0754820 | Msa1368360 | 0.828881 | 7.026226e-55 | -8.615850e-47 |
| Msa0754820 | Msa1398090 | 0.802660 | 5.049400e-49 | -8.615850e-47 |
| Msa0754820 | Msa1407190 | 0.816302 | 5.932780e-52 | -8.615850e-47 |
| Msa0754820 | Msa1412130 | 0.833491 | 5.173772e-56 | -8.615850e-47 |
| Msa0754820 | Msa1425260 | 0.824763 | 6.766952e-54 | -8.615850e-47 |
| Msa0754820 | Msa1432130 | 0.811448 | 6.966132e-51 | -8.615850e-47 |
| Msa0754820 | Msa1463900 | 0.806824 | 6.816882e-50 | -8.615850e-47 |
| Msa0127780 | Msa0754820 | 0.812677 | 3.759559e-51 | -8.615850e-47 |
| Msa0138020 | Msa0754820 | 0.809342 | 1.983926e-50 | -8.615850e-47 |
| Msa0144530 | Msa0754820 | 0.840898 | 6.586799e-58 | -8.615850e-47 |
| Msa0148080 | Msa0754820 | 0.823228 | 1.551035e-53 | -8.615850e-47 |
| Msa0204960 | Msa0754820 | 0.821541 | 3.821848e-53 | -8.615850e-47 |
| Msa0206830 | Msa0754820 | 0.801106 | 1.053123e-48 | -8.615850e-47 |
| Msa0427580 | Msa0754820 | 0.813056 | 3.104379e-51 | -8.615850e-47 |
| Msa0481030 | Msa0754820 | 0.804442 | 2.156432e-49 | -8.615850e-47 |
| Msa0241140 | Msa0754820 | 0.803243 | 3.826461e-49 | -8.615850e-47 |
| Msa0295460 | Msa0754820 | -0.822943 | 1.807246e-53 | -8.615850e-47 |
| Msa0305410 | Msa0754820 | 0.807311 | 5.378866e-50 | -8.615850e-47 |
| Msa0321690 | Msa0754820 | 0.801826 | 7.497435e-49 | -8.615850e-47 |
| Msa0333970 | Msa0754820 | -0.804234 | 2.382465e-49 | -8.615850e-47 |
| Msa0363790 | Msa0754820 | 0.802764 | 4.805440e-49 | -8.615850e-47 |
| Msa0518170 | Msa0754820 | 0.830337 | 3.107993e-55 | -8.615850e-47 |
| Msa0519160 | Msa0754820 | 0.809981 | 1.446231e-50 | -8.615850e-47 |
| Msa0600080 | Msa0754820 | 0.807720 | 4.402925e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754820 | MtrunA17_Chr5g0399651 | 98.246 | 57 | 1 | 0 | 1 | 57 | 1 | 57 | 1.16e-35 | 114 |
| Msa0754820 | MtrunA17_Chr1g0196811 | 55.556 | 45 | 20 | 0 | 9 | 53 | 37 | 81 | 2.87e-14 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0754820 | AT2G35658.3 | 53.571 | 56 | 25 | 1 | 2 | 56 | 33 | 88 | 7.06e-18 | 70.5 |
| Msa0754820 | AT2G35658.1 | 52.830 | 53 | 24 | 1 | 2 | 53 | 33 | 85 | 1.79e-16 | 66.6 |
| Msa0754820 | AT2G35658.2 | 50.909 | 55 | 26 | 1 | 2 | 55 | 33 | 87 | 1.82e-16 | 67.0 |
Find 28 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATAAGATGAACAGCTTTC+TGG | 0.180591 | 5_2:-8531003 | None:intergenic |
| TTTCATTATATTAGATTGTT+TGG | 0.314457 | 5_2:+8531253 | Msa0754820:three_prime_UTR |
| CTAAATTAGTTGGAGTGGTT+TGG | 0.336990 | 5_2:+8530845 | Msa0754820:five_prime_UTR |
| TTCTTTGTGCTATCATCCTC+TGG | 0.356172 | 5_2:+8531038 | Msa0754820:CDS |
| GTTCTTCTCTTCCCCAACTA+AGG | 0.380504 | 5_2:+8531060 | Msa0754820:CDS |
| GAATTTGTGTATCAATAGAT+TGG | 0.395682 | 5_2:+8531366 | Msa0754820:three_prime_UTR |
| GATAAGATGAACAGCTTTCT+GGG | 0.396373 | 5_2:-8531002 | None:intergenic |
| AACAGAAGAGAACCTTAGTT+GGG | 0.415115 | 5_2:-8531072 | None:intergenic |
| TATATCACTGGAATCTAGAT+AGG | 0.428049 | 5_2:+8531344 | Msa0754820:three_prime_UTR |
| TCTCTCCCATGTTGCAAAGA+AGG | 0.432445 | 5_2:+8530958 | Msa0754820:CDS |
| ATGCATAGTTTCAGCATAAG+AGG | 0.446059 | 5_2:+8531193 | Msa0754820:three_prime_UTR |
| CTCCAACTAATTTAGTTCGC+TGG | 0.460222 | 5_2:-8530837 | None:intergenic |
| AGCCAGCGAACTAAATTAGT+TGG | 0.462152 | 5_2:+8530835 | Msa0754820:five_prime_UTR |
| ATTTGTACCAACTATATCAC+TGG | 0.477992 | 5_2:+8531332 | Msa0754820:three_prime_UTR |
| AAACAGAAGAGAACCTTAGT+TGG | 0.485222 | 5_2:-8531073 | None:intergenic |
| GAGGATCGTATGCTGGTAGT+AGG | 0.508758 | 5_2:-8530937 | None:intergenic |
| CTAGATTCCAGTGATATAGT+TGG | 0.510649 | 5_2:-8531339 | None:intergenic |
| CAGATTACATAATTTGATGA+CGG | 0.538839 | 5_2:+8531170 | Msa0754820:intron |
| GCACAAAGAAGAACAAGAAG+AGG | 0.551389 | 5_2:-8531025 | None:intergenic |
| TTCTTTGCAACATGGGAGAG+AGG | 0.554290 | 5_2:-8530956 | None:intergenic |
| CAGTTCCTTCTTTGCAACAT+GGG | 0.554330 | 5_2:-8530963 | None:intergenic |
| TGGGAGAGAGGATCGTATGC+TGG | 0.560198 | 5_2:-8530944 | None:intergenic |
| GCGAACTAAATTAGTTGGAG+TGG | 0.562737 | 5_2:+8530840 | Msa0754820:five_prime_UTR |
| ACAGTTCCTTCTTTGCAACA+TGG | 0.577429 | 5_2:-8530964 | None:intergenic |
| CACAAAGAAGAACAAGAAGA+GGG | 0.602024 | 5_2:-8531024 | None:intergenic |
| TTGGGGAAGAGAAGAACCAG+AGG | 0.622299 | 5_2:-8531054 | None:intergenic |
| ACAGAAGAGAACCTTAGTTG+GGG | 0.648692 | 5_2:-8531071 | None:intergenic |
| TAGAAACAAACAAACAAACA+TGG | 0.652612 | 5_2:+8530898 | Msa0754820:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTCATTATATTAGATTGTT+TGG | + | chr5_2:8531253-8531272 | Msa0754820:three_prime_UTR | 15.0% |
| !! | AAGTTATCATACAATAAAAA+TGG | - | chr5_2:8531423-8531442 | None:intergenic | 15.0% |
| !!! | ACTATTTCGTGTTTTTATTT+TGG | - | chr5_2:8530798-8530817 | None:intergenic | 20.0% |
| !!! | TTCAATTTTCAGTTTTAGAT+TGG | + | chr5_2:8531097-8531116 | Msa0754820:intron | 20.0% |
| !! | ATTAAAGTAGTTAGCAAATA+TGG | - | chr5_2:8531133-8531152 | None:intergenic | 20.0% |
| ! | TAGAAACAAACAAACAAACA+TGG | + | chr5_2:8530898-8530917 | Msa0754820:exon | 25.0% |
| ! | CAGATTACATAATTTGATGA+CGG | + | chr5_2:8531170-8531189 | Msa0754820:intron | 25.0% |
| ! | GAATTTGTGTATCAATAGAT+TGG | + | chr5_2:8531366-8531385 | Msa0754820:three_prime_UTR | 25.0% |
| ATTTGTACCAACTATATCAC+TGG | + | chr5_2:8531332-8531351 | Msa0754820:three_prime_UTR | 30.0% | |
| TATATCACTGGAATCTAGAT+AGG | + | chr5_2:8531344-8531363 | Msa0754820:three_prime_UTR | 30.0% | |
| CTAAATTAGTTGGAGTGGTT+TGG | + | chr5_2:8530845-8530864 | Msa0754820:five_prime_UTR | 35.0% | |
| GATAAGATGAACAGCTTTCT+GGG | - | chr5_2:8531005-8531024 | None:intergenic | 35.0% | |
| CACAAAGAAGAACAAGAAGA+GGG | - | chr5_2:8531027-8531046 | None:intergenic | 35.0% | |
| AACAGAAGAGAACCTTAGTT+GGG | - | chr5_2:8531075-8531094 | None:intergenic | 35.0% | |
| AAACAGAAGAGAACCTTAGT+TGG | - | chr5_2:8531076-8531095 | None:intergenic | 35.0% | |
| ATGCATAGTTTCAGCATAAG+AGG | + | chr5_2:8531193-8531212 | Msa0754820:three_prime_UTR | 35.0% | |
| CTAGATTCCAGTGATATAGT+TGG | - | chr5_2:8531342-8531361 | None:intergenic | 35.0% | |
| AGCCAGCGAACTAAATTAGT+TGG | + | chr5_2:8530835-8530854 | Msa0754820:five_prime_UTR | 40.0% | |
| ! | CTCCAACTAATTTAGTTCGC+TGG | - | chr5_2:8530840-8530859 | None:intergenic | 40.0% |
| GCGAACTAAATTAGTTGGAG+TGG | + | chr5_2:8530840-8530859 | Msa0754820:five_prime_UTR | 40.0% | |
| CAGTTCCTTCTTTGCAACAT+GGG | - | chr5_2:8530966-8530985 | None:intergenic | 40.0% | |
| ACAGTTCCTTCTTTGCAACA+TGG | - | chr5_2:8530967-8530986 | None:intergenic | 40.0% | |
| GGATAAGATGAACAGCTTTC+TGG | - | chr5_2:8531006-8531025 | None:intergenic | 40.0% | |
| GCACAAAGAAGAACAAGAAG+AGG | - | chr5_2:8531028-8531047 | None:intergenic | 40.0% | |
| TTCTTTGTGCTATCATCCTC+TGG | + | chr5_2:8531038-8531057 | Msa0754820:CDS | 40.0% | |
| ACAGAAGAGAACCTTAGTTG+GGG | - | chr5_2:8531074-8531093 | None:intergenic | 40.0% | |
| TTCTTTGCAACATGGGAGAG+AGG | - | chr5_2:8530959-8530978 | None:intergenic | 45.0% | |
| TCTCTCCCATGTTGCAAAGA+AGG | + | chr5_2:8530958-8530977 | Msa0754820:CDS | 45.0% | |
| GTTCTTCTCTTCCCCAACTA+AGG | + | chr5_2:8531060-8531079 | Msa0754820:CDS | 45.0% | |
| GAGGATCGTATGCTGGTAGT+AGG | - | chr5_2:8530940-8530959 | None:intergenic | 50.0% | |
| TTGGGGAAGAGAAGAACCAG+AGG | - | chr5_2:8531057-8531076 | None:intergenic | 50.0% | |
| TGGGAGAGAGGATCGTATGC+TGG | - | chr5_2:8530947-8530966 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 8530788 | 8531483 | 8530788 | ID=Msa0754820;Name=Msa0754820 |
| chr5_2 | mRNA | 8530788 | 8531483 | 8530788 | ID=Msa0754820-mRNA-1;Parent=Msa0754820;Name=Msa0754820-mRNA-1;_AED=0.00;_eAED=0.00;_QI=129|1|1|1|1|1|2|302|57 |
| chr5_2 | exon | 8530788 | 8531081 | 8530788 | ID=Msa0754820-mRNA-1:exon:3859;Parent=Msa0754820-mRNA-1 |
| chr5_2 | exon | 8531173 | 8531483 | 8531173 | ID=Msa0754820-mRNA-1:exon:3860;Parent=Msa0754820-mRNA-1 |
| chr5_2 | five_prime_UTR | 8530788 | 8530916 | 8530788 | ID=Msa0754820-mRNA-1:five_prime_utr;Parent=Msa0754820-mRNA-1 |
| chr5_2 | CDS | 8530917 | 8531081 | 8530917 | ID=Msa0754820-mRNA-1:cds;Parent=Msa0754820-mRNA-1 |
| chr5_2 | CDS | 8531173 | 8531181 | 8531173 | ID=Msa0754820-mRNA-1:cds;Parent=Msa0754820-mRNA-1 |
| chr5_2 | three_prime_UTR | 8531182 | 8531483 | 8531182 | ID=Msa0754820-mRNA-1:three_prime_utr;Parent=Msa0754820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
