AlfalfaGEDB | Msa0810190

M. sativa cultivar zhongmu-4 / Msa0810190

KEGG: -
GO: -
PFAM: PF04674.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810190	RHN56160.1	67.678	659	125	6	3	574	301	958	0.0	818
Msa0810190	RHN56160.1	79.545	88	15	1	55	139	47	134	3.83e-30	138
Msa0810190	RHN56160.1	89.908	436	20	2	139	574	237	648	0.0	788
Msa0810190	RHN56160.1	91.850	319	25	1	256	574	22	339	0.0	597

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810190	sp\|O82161\|PHI1_TOBAC	74.359	312	71	4	267	574	7	313	1.36e-161	466
Msa0810190	sp\|O82161\|PHI1_TOBAC	68.800	125	16	1	140	264	212	313	8.70e-47	169

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810190	A0A396HS48	67.678	659	125	6	3	574	301	958	0.0	818
Msa0810190	A0A396HS48	79.545	88	15	1	55	139	47	134	1.83e-30	138
Msa0810190	A0A396HS48	89.908	436	20	2	139	574	237	648	0.0	788
Msa0810190	A0A396HS48	91.850	319	25	1	256	574	22	339	0.0	597

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0731870	Msa0810190	0.849796	2.569517e-60	-8.615850e-47
Msa0731900	Msa0810190	0.857979	1.127930e-62	-8.615850e-47
Msa0731990	Msa0810190	0.828114	1.076346e-54	-8.615850e-47
Msa0732010	Msa0810190	0.824412	8.184224e-54	-8.615850e-47
Msa0772940	Msa0810190	0.843696	1.195542e-58	-8.615850e-47
Msa0810190	Msa0853250	0.877009	8.856963e-69	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810190	MtrunA17_Chr5g0426441	67.678	659	125	6	3	574	301	958	0.0	818
Msa0810190	MtrunA17_Chr5g0426441	89.908	436	20	2	139	574	237	648	0.0	788
Msa0810190	MtrunA17_Chr5g0426441	91.850	319	25	1	256	574	22	339	0.0	597
Msa0810190	MtrunA17_Chr5g0426441	79.545	88	15	1	55	139	47	134	3.52e-34	138
Msa0810190	MtrunA17_Chr3g0116001	79.461	297	48	3	278	574	21	304	3.57e-169	482
Msa0810190	MtrunA17_Chr3g0116001	43.463	283	84	7	55	264	25	304	6.57e-51	177
Msa0810190	MtrunA17_Chr3g0116011	78.344	314	55	4	261	574	4	304	2.93e-168	479
Msa0810190	MtrunA17_Chr3g0116011	72.000	125	12	1	140	264	203	304	1.93e-50	176
Msa0810190	MtrunA17_Chr3g0115951	71.383	311	85	3	267	574	8	317	1.31e-152	440
Msa0810190	MtrunA17_Chr3g0115951	69.841	126	15	1	139	264	215	317	8.07e-50	174

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0810190	AT4G08950.1	71.906	299	74	6	282	574	20	314	5.07e-145	421
Msa0810190	AT4G08950.1	67.200	125	18	1	140	264	213	314	1.18e-46	166
Msa0810190	AT4G08950.1	59.783	92	31	4	55	141	20	110	2.47e-22	98.6
Msa0810190	AT1G35140.1	64.952	311	106	2	263	573	1	308	8.44e-140	408
Msa0810190	AT1G35140.1	64.000	125	22	1	140	264	208	309	8.14e-45	161
Msa0810190	AT1G35140.1	54.651	86	39	0	42	127	6	91	3.60e-24	103
Msa0810190	AT5G64260.1	57.792	308	120	4	268	574	7	305	2.37e-118	353
Msa0810190	AT5G64260.1	56.800	125	31	1	140	264	204	305	2.91e-38	143
Msa0810190	AT5G64260.1	48.810	84	42	1	44	127	10	92	2.82e-19	89.4
Msa0810190	AT5G09440.1	55.814	301	99	7	276	574	10	278	3.61e-99	302

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Find 135 sgRNAs with CRISPR-Local

Find 817 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAACCAAATTCCAA+CGG	0.640033	5_3:-38263377	None:intergenic
AAAAGGGCCCCAGCATAACC+AGG	0.566264	5_3:-38271010	None:intergenic
AAAAGGTCCCCAGCATAACC+AGG	0.566674	5_3:-38267424	None:intergenic
AAAAGGTCCCCAGCATAACC+AGG	0.566674	5_3:-38276856	None:intergenic
AAACAAATTTGCGTACATTT+GGG	0.353432	5_3:+38276582	Msa0810190:CDS
AAATCTCAATCAACCTCATT+TGG	0.382865	5_3:+38265423	Msa0810190:CDS
AAATCTCAATCAACCTCATT+TGG	0.382865	5_3:+38276182	Msa0810190:CDS
AAATTTGCGTACATTTGGGT+TGG	0.481339	5_3:+38276586	Msa0810190:CDS
AACCATCAGTTGCCACGTGG+TGG	0.610757	5_3:+38265534	Msa0810190:CDS
AACCATCAGTTGCCACGTGG+TGG	0.610757	5_3:+38276293	Msa0810190:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTAAATGCAAGTTTCCGT+TGG	+	chr5_3:38263361-38263380	Msa0810190:CDS	30.0%
ATATACTCTACTGGTAGATA+AGG	-	chr5_3:38263617-38263636	None:intergenic	30.0%
CTTATATTAAAGCTGGACTA+CGG	-	chr5_3:38263942-38263961	None:intergenic	30.0%
TATCCCACTTATATTAAAGC+TGG	-	chr5_3:38263949-38263968	None:intergenic	30.0%
ATTATTCTCGTAGTGATAAG+TGG	+	chr5_3:38264132-38264151	Msa0810190:intron	30.0%
ATTGTTACGCTCTTCAATAA+TGG	+	chr5_3:38264898-38264917	Msa0810190:intron	30.0%
ATCTATGAATTCATGCAAAC+TGG	+	chr5_3:38265032-38265051	Msa0810190:intron	30.0%
TGAAGTCAAATTATGTAGGA+GGG	-	chr5_3:38265107-38265126	None:intergenic	30.0%
TTGAAGTCAAATTATGTAGG+AGG	-	chr5_3:38265108-38265127	None:intergenic	30.0%
GTAGTGAAATTGTGAGAAAA+AGG	-	chr5_3:38265316-38265335	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	38263263	38276990	38263263	ID=Msa0810190;Name=Msa0810190
chr5_3	mRNA	38263263	38276990	38263263	ID=Msa0810190-mRNA-1;Parent=Msa0810190;Name=Msa0810190-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0\|0\|0.8\|0\|0\|5\|0\|574
chr5_3	exon	38263263	38263428	38263263	ID=Msa0810190-mRNA-1:exon:11700;Parent=Msa0810190-mRNA-1
chr5_3	exon	38265354	38265600	38265354	ID=Msa0810190-mRNA-1:exon:11701;Parent=Msa0810190-mRNA-1
chr5_3	exon	38267246	38267484	38267246	ID=Msa0810190-mRNA-1:exon:11702;Parent=Msa0810190-mRNA-1
chr5_3	exon	38271002	38271138	38271002	ID=Msa0810190-mRNA-1:exon:11703;Parent=Msa0810190-mRNA-1
chr5_3	exon	38276055	38276990	38276055	ID=Msa0810190-mRNA-1:exon:11704;Parent=Msa0810190-mRNA-1
chr5_3	CDS	38263263	38263428	38263263	ID=Msa0810190-mRNA-1:cds;Parent=Msa0810190-mRNA-1
chr5_3	CDS	38265354	38265600	38265354	ID=Msa0810190-mRNA-1:cds;Parent=Msa0810190-mRNA-1
chr5_3	CDS	38267246	38267484	38267246	ID=Msa0810190-mRNA-1:cds;Parent=Msa0810190-mRNA-1
chr5_3	CDS	38271002	38271138	38271002	ID=Msa0810190-mRNA-1:cds;Parent=Msa0810190-mRNA-1
chr5_3	CDS	38276055	38276990	38276055	ID=Msa0810190-mRNA-1:cds;Parent=Msa0810190-mRNA-1

Gene Sequence

>Msa0810190

ATGACGGATATGACGGTGGAAAAGGACGACGGTGATGAAGTTGGTGAACGTCGGTGCCAGAAGTTGTTACAGGCGACGGTGAGGTTGTTACAGGCGAGAATTAAATGCAAGTTTCCGTTGGAATTTGGTTTTGTTTTGCTCTTAGGTGAAGAAAGCAATTTCAGTGCTAGAAAACTCACTGAATCCGATCAACAACTTAAATTTCAATACCACAAAGGCCCTCTTCTCACTGGTAAAATCTCAATCAACCTCATTTGGTATGGAAAATTCAAACCATCCCAAAGAGCCATCATAACTGATTTCATAACCTCCCTTTCTTCTTCCAAAAAACAAACAGCCACATCACAACCATCAGTTGCCACGTGGTGGAAATCAACTAAAAATATTACCAACTCACTAGCAACAAGAAATCATGCACCATTGGTTGCACCAAATAACGATGTGGGTCTTGATGGAATGGTTATAAATGTCGCTAGTCTTTTAGCTGGAACCGTGACCAATCCTTTTGGTAATGGATATTTTCAAGGACCAAAAGAAGCTCCTTTGGAAGCTGCTTCGGCTTGTACCGGTGTTTATGCTAAAGGAGCTTATCCTGGTTATGCTGGGGACCTTTTGGTGGACAAAACAAGTGGTGCTAGCTATAACGCAAATGGAGCTTATCCTGGTTATGCTGGGGCCCTTTTGGTGGACAAAACAAGTGGTGCTAGCTACAACGCTAATGGTGATAATGGAAGGAAGTATTTGCTGCCTGCGATTGTTGATCCTAAAACTTCAGCTTGTTCTACATTGTTAAGTGTAATCTTCACTCTTTTTCTCACAATTTCACTGCTTCATTTAATCTCAGCTGCAAGAAAACTCACCGAATCGGACCAACAACTTAAATTTCAATACCACAAAGGCCCTCTTCTCACTGGCAAAATCTCAATCAACCTCATTTGGTATGGAAAATTCAAACCGTCCCAAAGAGTCATCATAACTGATTTCATAACCTCCCTCTCTTCTCCTAAACGAGGAACAACCACGTCACAACCATCAGTTGCCACGTGGTGGAAATCAACTGAAAAATATTACCAACTCACCAACAATAAAAAGTCAGCTAAACTAGCTCTCTCACTAGGTGCTCAAATTCTCGATGAGAATTACTCTTTAGGAAAATCTCTCACAACTAACCAAATCCTTAAACTCGCATCAAAAGGACAACAACAAAACGCCATCAACGTTGTCCTAACAGCTGCTGATGTGTTAGTTGACGGTTTTTGTTCAAGTAGGTGTGGAACCCATGGTTCTTCTTATGGTGCACGCGTAAATGGAAAAAGAAACAAATTTGCGTACATTTGGGTTGGAAATTCAGAGACACAATGTGCTGGTCAATGCGCGTGGCCATTTCACCAACCAATTTATGGTCCACAAAGTGCACCATTGGTTGCACCAAATAACGATGTGGGTTTTGATGGAATGGTTATAAATGTTGCTAGTCTTTTAGCTGGAACCGTAACGAATCCTTTTGGTAATGGATATTTTCAAGGACCAAAAGAAGCTCCTTTGGAAGCTGCTTCAGCTTGTACTGGTGTTTATGCTAAAGGGGCTTATCCTGGTTATGCTGGGGACCTTTTGGTGGACAAAACAAGTGGTGCTAGCTATAACGCAAATGGTGATAATGGAAGGAAGTATTTGTTGCCTGCGATTGTTGATCCTAAAACCTCAGTTTGTTCTACGTTGGTATAA

Protein sequence

>Msa0810190

MTDMTVEKDDGDEVGERRCQKLLQATVRLLQARIKCKFPLEFGFVLLLGEESNFSARKLTESDQQLKFQYHKGPLLTGKISINLIWYGKFKPSQRAIITDFITSLSSSKKQTATSQPSVATWWKSTKNITNSLATRNHAPLVAPNNDVGLDGMVINVASLLAGTVTNPFGNGYFQGPKEAPLEAASACTGVYAKGAYPGYAGDLLVDKTSGASYNANGAYPGYAGALLVDKTSGASYNANGDNGRKYLLPAIVDPKTSACSTLLSVIFTLFLTISLLHLISAARKLTESDQQLKFQYHKGPLLTGKISINLIWYGKFKPSQRVIITDFITSLSSPKRGTTTSQPSVATWWKSTEKYYQLTNNKKSAKLALSLGAQILDENYSLGKSLTTNQILKLASKGQQQNAINVVLTAADVLVDGFCSSRCGTHGSSYGARVNGKRNKFAYIWVGNSETQCAGQCAWPFHQPIYGPQSAPLVAPNNDVGFDGMVINVASLLAGTVTNPFGNGYFQGPKEAPLEAASACTGVYAKGAYPGYAGDLLVDKTSGASYNANGDNGRKYLLPAIVDPKTSVCSTLV*