AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0822570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0822570 MtrunA17_Chr5g0442931 100.000 178 0 0 1 178 1 178 2.99e-133 370
Msa0822570 MtrunA17_Chr5g0442901 100.000 178 0 0 1 178 1 178 2.99e-133 370
Msa0822570 MtrunA17_Chr2g0328821 100.000 178 0 0 1 178 17 194 3.33e-133 370
Msa0822570 MtrunA17_Chr3g0095911 94.382 178 10 0 1 178 1 178 2.58e-125 350
Msa0822570 MtrunA17_Chr2g0286131 62.353 85 32 0 92 176 1 85 2.93e-34 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0822570 AT2G34480.1 90.230 174 17 0 5 178 5 178 3.01e-119 335
Msa0822570 AT2G34480.2 90.230 174 17 0 5 178 44 217 3.24e-119 336
Msa0822570 AT1G29965.1 87.079 178 23 0 1 178 1 178 1.24e-117 330
Msa0822570 AT3G14600.1 87.079 178 23 0 1 178 1 178 4.20e-117 329
Msa0822570 AT1G29970.2 89.017 173 19 0 6 178 140 312 1.08e-114 328

Find 63 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAACAGGTTTGGCTTCT+TGG 0.139462 5_3:-60991614 None:intergenic
ATTAGAAACTTACCAGAATT+TGG 0.167108 5_3:-60989684 None:intergenic
CACCAGAGGGAACTTGATTT+TGG 0.187302 5_3:-60991542 None:intergenic
AGATTAAGAACTATGGAATT+TGG 0.243479 5_3:+60991320 Msa0822570:CDS
GAAAAGTAATGGACAAGTTT+TGG 0.246012 5_3:+60989831 Msa0822570:CDS
GTTTGGCTTCTTGGCTTTGT+AGG 0.246112 5_3:-60991605 None:intergenic
CACATTTCGCTTCTCATATT+GGG 0.263107 5_3:+60991650 Msa0822570:three_prime_UTR
TCACATTTCGCTTCTCATAT+TGG 0.265029 5_3:+60991649 Msa0822570:three_prime_UTR
TGTTTGTGCACTCTTATTGC+TGG 0.328496 5_3:+60991903 Msa0822570:three_prime_UTR
ACTCTTATTGCTGGATAAAA+AGG 0.328695 5_3:+60991912 Msa0822570:three_prime_UTR
AGATTTATCGCATGAAGCTT+TGG 0.341025 5_3:+60989636 Msa0822570:CDS
AACTTACCAGAATTTGGATT+TGG 0.348831 5_3:-60989678 None:intergenic
ACGAGATGGCATCTCGTCAT+AGG 0.358917 5_3:+60991431 Msa0822570:CDS
CCTACCAAGATTAAGAACTA+TGG 0.366976 5_3:+60991313 Msa0822570:CDS
GATTTATCGCATGAAGCTTT+GGG 0.367321 5_3:+60989637 Msa0822570:CDS
ATTCACTGAATCACATAAAC+AGG 0.373443 5_3:-60991627 None:intergenic
TTCGTGCCAAATCCAAATTC+TGG 0.381119 5_3:+60989672 Msa0822570:CDS
AAGCTTCATGCGATAAATCT+TGG 0.383045 5_3:-60989633 None:intergenic
TCCCAGCTAATCTGTGCAAA+AGG 0.391912 5_3:+60991494 Msa0822570:CDS
CTGAATCACATAAACAGGTT+TGG 0.397820 5_3:-60991622 None:intergenic
AATTCCATAGTTCTTAATCT+TGG 0.403701 5_3:-60991317 None:intergenic
GCTCATTAGCTATGAGCATC+TGG 0.427165 5_3:-60991944 None:intergenic
AAAAGATAAAACAAAATTCT+CGG 0.433849 5_3:-60991778 None:intergenic
TACCGTGATACTACTCTAAA+CGG 0.441043 5_3:+60991388 Msa0822570:CDS
GGATTTGGCACGAACCTCAT+TGG 0.442665 5_3:-60989663 None:intergenic
AGTCTTAATGATTTGAATGC+AGG 0.447032 5_3:-60991464 None:intergenic
CTCTGGTGTACAAGAAGATT+AGG 0.461451 5_3:+60991557 Msa0822570:CDS
ACTACAATCAGCGGCGTTCA+CGG 0.466844 5_3:+60989434 Msa0822570:five_prime_UTR
AGCTTCATGCGATAAATCTT+GGG 0.476384 5_3:-60989632 None:intergenic
CTCTTCCAACAACCTGGTAC+TGG 0.489846 5_3:-60989583 None:intergenic
TTTCTTGAGGAAGTTGAAGA+AGG 0.490067 5_3:+60989804 Msa0822570:CDS
TGGCATCTCGTCATAGGGTC+AGG 0.497058 5_3:+60991437 Msa0822570:CDS
TTTCACCAGTACCAGGTTGT+TGG 0.499494 5_3:+60989578 Msa0822570:CDS
TTGCGTTATCAGAGTCGTAC+TGG 0.505845 5_3:+60991343 Msa0822570:CDS
TGAATCAAGCAGATGGAAGC+AGG 0.512111 5_3:-60989398 None:intergenic
TAGCGATCACTTTATTTCGA+AGG 0.517130 5_3:+60991861 Msa0822570:three_prime_UTR
AAGAAGGTTAAGAAAAGTAA+TGG 0.518066 5_3:+60989820 Msa0822570:CDS
TTTGCACAGATTAGCTGGGA+TGG 0.520373 5_3:-60991491 None:intergenic
AAAATTCTCGGTAAGCAACT+AGG 0.521581 5_3:-60991766 None:intergenic
CCCAGCTAATCTGTGCAAAA+GGG 0.524734 5_3:+60991495 Msa0822570:CDS
TGGGAAGAACAAAGCTGTGT+TGG 0.528015 5_3:+60991981 Msa0822570:three_prime_UTR
CGATCACTTTATTTCGAAGG+AGG 0.532163 5_3:+60991864 Msa0822570:three_prime_UTR
AAGAGCACTTCCCACCGAAA+AGG 0.541834 5_3:+60989601 Msa0822570:CDS
CGAGATGGCATCTCGTCATA+GGG 0.544710 5_3:+60991432 Msa0822570:CDS
CCATAGTTCTTAATCTTGGT+AGG 0.548600 5_3:-60991313 None:intergenic
GAAACAGTTTCACCAGTACC+AGG 0.549157 5_3:+60989571 Msa0822570:intron
TTCCAAAATCAAGTTCCCTC+TGG 0.549470 5_3:+60991540 Msa0822570:CDS
GATCACTTTATTTCGAAGGA+GGG 0.564288 5_3:+60991865 Msa0822570:three_prime_UTR
GTCTTAATGATTTGAATGCA+GGG 0.566014 5_3:-60991463 None:intergenic
ATGCTCATAGCTAATGAGCT+AGG 0.567864 5_3:+60991948 Msa0822570:three_prime_UTR
TCAAGGGTGAATCAAGCAGA+TGG 0.585314 5_3:-60989405 None:intergenic
AGAAGATTAGGCCACCAACT+AGG 0.603627 5_3:+60991569 Msa0822570:CDS
GCACAGATTAGCTGGGATGG+TGG 0.612537 5_3:-60991488 None:intergenic
TGGTTATCACAATATGTACA+AGG 0.613559 5_3:+60991363 Msa0822570:CDS
GAAGTGCTCTTCCAACAACC+TGG 0.619307 5_3:-60989589 None:intergenic
GAAGCTTTGGGCAACCAATG+AGG 0.630309 5_3:+60989649 Msa0822570:CDS
CTAATCTTCTTGTACACCAG+AGG 0.631597 5_3:-60991556 None:intergenic
TAATCTTCTTGTACACCAGA+GGG 0.642722 5_3:-60991555 None:intergenic
ATTGTTGTAGGTATTTCTTG+AGG 0.651477 5_3:+60989791 Msa0822570:intron
GGAGACAATGTACAACGAGA+TGG 0.663821 5_3:+60991417 Msa0822570:CDS
CGCCGTTTAGAGTAGTATCA+CGG 0.671590 5_3:-60991390 None:intergenic
TACTACTCTAAACGGCGCTG+TGG 0.679213 5_3:+60991396 Msa0822570:CDS
TGACGCAAAACTACAATCAG+CGG 0.685068 5_3:+60989425 Msa0822570:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTGAAATTTTATAGAA+GGG + chr5_3:60989891-60989910 Msa0822570:intron 10.0%
!! ACAAAATACAATAAAAAAAA+GGG - chr5_3:60991134-60991153 None:intergenic 10.0%
!! AACAAAATACAATAAAAAAA+AGG - chr5_3:60991135-60991154 None:intergenic 10.0%
!! TTAATAATGTTAATTGTTGT+AGG + chr5_3:60989779-60989798 Msa0822570:intron 15.0%
!! TCTATAAAATTTCAATTAAC+AGG - chr5_3:60989890-60989909 None:intergenic 15.0%
!!! GTTAATTGAAATTTTATAGA+AGG + chr5_3:60989890-60989909 Msa0822570:intron 15.0%
!! GAATAGAAAAAATTAATGAT+AGG + chr5_3:60990202-60990221 Msa0822570:intron 15.0%
!! AAGCAACAAATAAAAAAAAA+TGG - chr5_3:60990792-60990811 None:intergenic 15.0%
!! CAAAATACAATAAAAAAAAG+GGG - chr5_3:60991133-60991152 None:intergenic 15.0%
!! AAAAGATAAAACAAAATTCT+CGG - chr5_3:60991781-60991800 None:intergenic 15.0%
!!! TTGTAGAGCTTTTTTTTTTT+AGG + chr5_3:60990011-60990030 Msa0822570:intron 20.0%
!!! TTTTTTTTTTAGGTAAAGCA+AGG + chr5_3:60990021-60990040 Msa0822570:intron 20.0%
!! ATATTAGTATATTGTACAGT+TGG + chr5_3:60990273-60990292 Msa0822570:intron 20.0%
!!! ATTTTAAGTAGATAGTACTT+TGG + chr5_3:60990854-60990873 Msa0822570:intron 20.0%
!! AGTCACAATATACAATTTAA+AGG - chr5_3:60991082-60991101 None:intergenic 20.0%
! CAAAATAAAATCAGCTCAAA+AGG - chr5_3:60989519-60989538 None:intergenic 25.0%
! ATTAGAAACTTACCAGAATT+TGG - chr5_3:60989687-60989706 None:intergenic 25.0%
! AAGAAGGTTAAGAAAAGTAA+TGG + chr5_3:60989820-60989839 Msa0822570:CDS 25.0%
! GTTAAGCTAGATTTATACTT+AGG + chr5_3:60989919-60989938 Msa0822570:intron 25.0%
! GTCAATAAAAACAAATAAGC+TGG - chr5_3:60989968-60989987 None:intergenic 25.0%
!! TGCATGTAAAAGCTTTTATT+TGG + chr5_3:60990106-60990125 Msa0822570:intron 25.0%
!! TCTATTTCTCTCATAAATAG+AGG + chr5_3:60990423-60990442 Msa0822570:intron 25.0%
!! CTATTTCTCTCATAAATAGA+GGG + chr5_3:60990424-60990443 Msa0822570:intron 25.0%
!! ATGTAATATCTGTCAGTTTT+TGG + chr5_3:60990514-60990533 Msa0822570:intron 25.0%
! TGAGAAAATTAAGTTTGATG+CGG + chr5_3:60990762-60990781 Msa0822570:intron 25.0%
! TTATGTTGAAATCCAAACAT+AGG + chr5_3:60991018-60991037 Msa0822570:intron 25.0%
! AATTCCATAGTTCTTAATCT+TGG - chr5_3:60991320-60991339 None:intergenic 25.0%
! AGATTAAGAACTATGGAATT+TGG + chr5_3:60991320-60991339 Msa0822570:CDS 25.0%
!! TTTTTCTCTTTATGATGTAC+TGG + chr5_3:60991818-60991837 Msa0822570:three_prime_UTR 25.0%
AATAAAATCAGCTCAAAAGG+TGG - chr5_3:60989516-60989535 None:intergenic 30.0%
! ATTTTGTGTGAATCGTAACA+CGG + chr5_3:60989533-60989552 Msa0822570:intron 30.0%
AACTTACCAGAATTTGGATT+TGG - chr5_3:60989681-60989700 None:intergenic 30.0%
ATTGTTGTAGGTATTTCTTG+AGG + chr5_3:60989791-60989810 Msa0822570:intron 30.0%
!!! GAAAAGTAATGGACAAGTTT+TGG + chr5_3:60989831-60989850 Msa0822570:CDS 30.0%
ATTTATACTTAGGCTGTGTT+TGG + chr5_3:60989929-60989948 Msa0822570:intron 30.0%
TAAACAAGTGCTTATAAGGT+AGG - chr5_3:60990138-60990157 None:intergenic 30.0%
TGGTTAAACAAGTGCTTATA+AGG - chr5_3:60990142-60990161 None:intergenic 30.0%
!! AGAGGAGTAAGTTATTTCTT+AGG + chr5_3:60990247-60990266 Msa0822570:intron 30.0%
TTGTGTCTATCTCTCATAAA+TGG - chr5_3:60990366-60990385 None:intergenic 30.0%
ACAACCAGAATATCATTAGA+GGG - chr5_3:60990402-60990421 None:intergenic 30.0%
TCTTCGAATAGACATCAAAA+AGG - chr5_3:60990652-60990671 None:intergenic 30.0%
TAAGTAGATAGTACTTTGGT+TGG + chr5_3:60990858-60990877 Msa0822570:intron 30.0%
!! ATAGTACTTTGGTTGGTAAA+GGG + chr5_3:60990865-60990884 Msa0822570:intron 30.0%
AAATGATGTATTGCCATAAC+TGG - chr5_3:60991047-60991066 None:intergenic 30.0%
AATTGTTGTGAGAGAATTCT+TGG + chr5_3:60991181-60991200 Msa0822570:intron 30.0%
TAATAGCTAAAGCAAACACA+GGG - chr5_3:60991217-60991236 None:intergenic 30.0%
! ACCTTCAATTTCCATCTTTT+TGG + chr5_3:60991241-60991260 Msa0822570:intron 30.0%
TCCAAAAAGATGGAAATTGA+AGG - chr5_3:60991245-60991264 None:intergenic 30.0%
! ATTTCCATCTTTTTGGACAT+AGG + chr5_3:60991248-60991267 Msa0822570:intron 30.0%
! TTTCCATCTTTTTGGACATA+GGG + chr5_3:60991249-60991268 Msa0822570:intron 30.0%
TGGTTATCACAATATGTACA+AGG + chr5_3:60991363-60991382 Msa0822570:CDS 30.0%
GTCTTAATGATTTGAATGCA+GGG - chr5_3:60991466-60991485 None:intergenic 30.0%
AGTCTTAATGATTTGAATGC+AGG - chr5_3:60991467-60991486 None:intergenic 30.0%
ATTCACTGAATCACATAAAC+AGG - chr5_3:60991630-60991649 None:intergenic 30.0%
ACTCTTATTGCTGGATAAAA+AGG + chr5_3:60991912-60991931 Msa0822570:three_prime_UTR 30.0%
TTATTGCTGGATAAAAAGGA+AGG + chr5_3:60991916-60991935 Msa0822570:three_prime_UTR 30.0%
!! TGATTGTAGTTTTGCGTCAA+GGG - chr5_3:60989424-60989443 None:intergenic 35.0%
AGCAAAAATGGTTAACGCTA+AGG + chr5_3:60989461-60989480 Msa0822570:exon 35.0%
! TTTTGTGTGAATCGTAACAC+GGG + chr5_3:60989534-60989553 Msa0822570:intron 35.0%
AGCTTCATGCGATAAATCTT+GGG - chr5_3:60989635-60989654 None:intergenic 35.0%
AAGCTTCATGCGATAAATCT+TGG - chr5_3:60989636-60989655 None:intergenic 35.0%
AGATTTATCGCATGAAGCTT+TGG + chr5_3:60989636-60989655 Msa0822570:CDS 35.0%
GATTTATCGCATGAAGCTTT+GGG + chr5_3:60989637-60989656 Msa0822570:CDS 35.0%
TTTCTTGAGGAAGTTGAAGA+AGG + chr5_3:60989804-60989823 Msa0822570:CDS 35.0%
!!! ACAAGTTTTGGCTATCAATG+AGG + chr5_3:60989843-60989862 Msa0822570:CDS 35.0%
!! TAACTCCACAGCTGTTTTAA+GGG - chr5_3:60990171-60990190 None:intergenic 35.0%
!! TTAACTCCACAGCTGTTTTA+AGG - chr5_3:60990172-60990191 None:intergenic 35.0%
CTGTGGAGTTAATGTTAAGT+TGG + chr5_3:60990180-60990199 Msa0822570:intron 35.0%
CATAAATGGAGGTAAATGGA+TGG - chr5_3:60990352-60990371 None:intergenic 35.0%
CTCTCATAAATGGAGGTAAA+TGG - chr5_3:60990356-60990375 None:intergenic 35.0%
TGTCTATCTCTCATAAATGG+AGG - chr5_3:60990363-60990382 None:intergenic 35.0%
CACAACCAGAATATCATTAG+AGG - chr5_3:60990403-60990422 None:intergenic 35.0%
AGAGCAAACTTGAACTCATA+TGG + chr5_3:60990456-60990475 Msa0822570:intron 35.0%
TGTGTACGAGGTAGATAAAT+GGG + chr5_3:60990719-60990738 Msa0822570:intron 35.0%
!! GATAGTACTTTGGTTGGTAA+AGG + chr5_3:60990864-60990883 Msa0822570:intron 35.0%
TGTTCTGTTGTGATTATTGC+TGG + chr5_3:60990917-60990936 Msa0822570:intron 35.0%
GTTCTGTTGTGATTATTGCT+GGG + chr5_3:60990918-60990937 Msa0822570:intron 35.0%
CATAACTGGTTACCTATGTT+TGG - chr5_3:60991033-60991052 None:intergenic 35.0%
CAAACATAGGTAACCAGTTA+TGG + chr5_3:60991031-60991050 Msa0822570:intron 35.0%
GTAATAGCTAAAGCAAACAC+AGG - chr5_3:60991218-60991237 None:intergenic 35.0%
! TTCCATCTTTTTGGACATAG+GGG + chr5_3:60991250-60991269 Msa0822570:intron 35.0%
CCATAGTTCTTAATCTTGGT+AGG - chr5_3:60991316-60991335 None:intergenic 35.0%
CCTACCAAGATTAAGAACTA+TGG + chr5_3:60991313-60991332 Msa0822570:CDS 35.0%
TACCGTGATACTACTCTAAA+CGG + chr5_3:60991388-60991407 Msa0822570:CDS 35.0%
TAATCTTCTTGTACACCAGA+GGG - chr5_3:60991558-60991577 None:intergenic 35.0%
!!! TGGCTTTGTAGGTTGTTTTT+AGG - chr5_3:60991597-60991616 None:intergenic 35.0%
CTGAATCACATAAACAGGTT+TGG - chr5_3:60991625-60991644 None:intergenic 35.0%
TCACATTTCGCTTCTCATAT+TGG + chr5_3:60991649-60991668 Msa0822570:three_prime_UTR 35.0%
CACATTTCGCTTCTCATATT+GGG + chr5_3:60991650-60991669 Msa0822570:three_prime_UTR 35.0%
AAAATTCTCGGTAAGCAACT+AGG - chr5_3:60991769-60991788 None:intergenic 35.0%
! TAGCGATCACTTTATTTCGA+AGG + chr5_3:60991861-60991880 Msa0822570:three_prime_UTR 35.0%
! GATCACTTTATTTCGAAGGA+GGG + chr5_3:60991865-60991884 Msa0822570:three_prime_UTR 35.0%
!! CTGATTGTAGTTTTGCGTCA+AGG - chr5_3:60989425-60989444 None:intergenic 40.0%
TGACGCAAAACTACAATCAG+CGG + chr5_3:60989425-60989444 Msa0822570:five_prime_UTR 40.0%
GTTCACGGTCATAGCAAAAA+TGG + chr5_3:60989449-60989468 Msa0822570:exon 40.0%
! CTTGGGATGTTCATCCTTTT+CGG - chr5_3:60989618-60989637 None:intergenic 40.0%
TTCGTGCCAAATCCAAATTC+TGG + chr5_3:60989672-60989691 Msa0822570:CDS 40.0%
TACTTAGGCTGTGTTTGGAT+TGG + chr5_3:60989934-60989953 Msa0822570:intron 40.0%
!!! AGCTGTTTTAAGGGACTGTT+TGG - chr5_3:60990162-60990181 None:intergenic 40.0%
! GAGTATAATTTTGCACCCTG+TGG + chr5_3:60990224-60990243 Msa0822570:intron 40.0%
ATAACTTACTCCTCTCCACA+GGG - chr5_3:60990242-60990261 None:intergenic 40.0%
AATAACTTACTCCTCTCCAC+AGG - chr5_3:60990243-60990262 None:intergenic 40.0%
GATAGACACAAACGATAGAG+TGG + chr5_3:60990375-60990394 Msa0822570:intron 40.0%
TGGACCCTCTAATGATATTC+TGG + chr5_3:60990395-60990414 Msa0822570:intron 40.0%
GCAAACTTGAACTCATATGG+CGG + chr5_3:60990459-60990478 Msa0822570:intron 40.0%
TGTAACCTTTCCCTAAGTCA+TGG + chr5_3:60990685-60990704 Msa0822570:intron 40.0%
CTAGATTCCACCATGACTTA+GGG - chr5_3:60990698-60990717 None:intergenic 40.0%
GTGTGTACGAGGTAGATAAA+TGG + chr5_3:60990718-60990737 Msa0822570:intron 40.0%
!! TACTTTGGTTGGTAAAGGGA+AGG + chr5_3:60990869-60990888 Msa0822570:intron 40.0%
TGTTACTCCGATTGCAAGTA+GGG + chr5_3:60990892-60990911 Msa0822570:intron 40.0%
TGTGAGAGAATTCTTGGAGT+AGG + chr5_3:60991187-60991206 Msa0822570:intron 40.0%
AACCCCTATGTCCAAAAAGA+TGG - chr5_3:60991255-60991274 None:intergenic 40.0%
TTCCAAAATCAAGTTCCCTC+TGG + chr5_3:60991540-60991559 Msa0822570:CDS 40.0%
CTAATCTTCTTGTACACCAG+AGG - chr5_3:60991559-60991578 None:intergenic 40.0%
CTCTGGTGTACAAGAAGATT+AGG + chr5_3:60991557-60991576 Msa0822570:CDS 40.0%
!!! GTTGTTTTTAGGCTCCTAGT+TGG - chr5_3:60991586-60991605 None:intergenic 40.0%
! CATAAACAGGTTTGGCTTCT+TGG - chr5_3:60991617-60991636 None:intergenic 40.0%
! CGATCACTTTATTTCGAAGG+AGG + chr5_3:60991864-60991883 Msa0822570:three_prime_UTR 40.0%
TGTTTGTGCACTCTTATTGC+TGG + chr5_3:60991903-60991922 Msa0822570:three_prime_UTR 40.0%
ATGCTCATAGCTAATGAGCT+AGG + chr5_3:60991948-60991967 Msa0822570:three_prime_UTR 40.0%
! ATGAGCTAGGTTTTGCGAAA+TGG + chr5_3:60991961-60991980 Msa0822570:three_prime_UTR 40.0%
! TGAGCTAGGTTTTGCGAAAT+GGG + chr5_3:60991962-60991981 Msa0822570:three_prime_UTR 40.0%
TCAAGGGTGAATCAAGCAGA+TGG - chr5_3:60989408-60989427 None:intergenic 45.0%
GAAACAGTTTCACCAGTACC+AGG + chr5_3:60989571-60989590 Msa0822570:intron 45.0%
TTTCACCAGTACCAGGTTGT+TGG + chr5_3:60989578-60989597 Msa0822570:CDS 45.0%
! GGATGTTCATCCTTTTCGGT+GGG - chr5_3:60989614-60989633 None:intergenic 45.0%
GACTTGCATAAGCGCTTATG+AGG + chr5_3:60989985-60990004 Msa0822570:intron 45.0%
ACAGTCCCTTAAAACAGCTG+TGG + chr5_3:60990163-60990182 Msa0822570:intron 45.0%
! TAATTTTGCACCCTGTGGAG+AGG + chr5_3:60990229-60990248 Msa0822570:intron 45.0%
! ATATGGCGGCGATTTGACTT+TGG + chr5_3:60990473-60990492 Msa0822570:intron 45.0%
! TATGGCGGCGATTTGACTTT+GGG + chr5_3:60990474-60990493 Msa0822570:intron 45.0%
AACCTTTCCCTAAGTCATGG+TGG + chr5_3:60990688-60990707 Msa0822570:intron 45.0%
TTCCACCATGACTTAGGGAA+AGG - chr5_3:60990693-60990712 None:intergenic 45.0%
CCTAGATTCCACCATGACTT+AGG - chr5_3:60990699-60990718 None:intergenic 45.0%
! CCTAAGTCATGGTGGAATCT+AGG + chr5_3:60990696-60990715 Msa0822570:intron 45.0%
! GTGTTACTCCGATTGCAAGT+AGG + chr5_3:60990891-60990910 Msa0822570:intron 45.0%
GAACAAGCCCTACTTGCAAT+CGG - chr5_3:60990902-60990921 None:intergenic 45.0%
TTGCGTTATCAGAGTCGTAC+TGG + chr5_3:60991343-60991362 Msa0822570:CDS 45.0%
! CGCCGTTTAGAGTAGTATCA+CGG - chr5_3:60991393-60991412 None:intergenic 45.0%
GGAGACAATGTACAACGAGA+TGG + chr5_3:60991417-60991436 Msa0822570:CDS 45.0%
! TTTGCACAGATTAGCTGGGA+TGG - chr5_3:60991494-60991513 None:intergenic 45.0%
TCCCAGCTAATCTGTGCAAA+AGG + chr5_3:60991494-60991513 Msa0822570:CDS 45.0%
!! CCCTTTTGCACAGATTAGCT+GGG - chr5_3:60991498-60991517 None:intergenic 45.0%
CCCAGCTAATCTGTGCAAAA+GGG + chr5_3:60991495-60991514 Msa0822570:CDS 45.0%
! TCCCTTTTGCACAGATTAGC+TGG - chr5_3:60991499-60991518 None:intergenic 45.0%
! CACCAGAGGGAACTTGATTT+TGG - chr5_3:60991545-60991564 None:intergenic 45.0%
AGAAGATTAGGCCACCAACT+AGG + chr5_3:60991569-60991588 Msa0822570:CDS 45.0%
!! GTTTTTAGGCTCCTAGTTGG+TGG - chr5_3:60991583-60991602 None:intergenic 45.0%
!! GTTTGGCTTCTTGGCTTTGT+AGG - chr5_3:60991608-60991627 None:intergenic 45.0%
GCTCATTAGCTATGAGCATC+TGG - chr5_3:60991947-60991966 None:intergenic 45.0%
TGGGAAGAACAAAGCTGTGT+TGG + chr5_3:60991981-60992000 Msa0822570:three_prime_UTR 45.0%
ACTACAATCAGCGGCGTTCA+CGG + chr5_3:60989434-60989453 Msa0822570:five_prime_UTR 50.0%
CTCTTCCAACAACCTGGTAC+TGG - chr5_3:60989586-60989605 None:intergenic 50.0%
! GAAGTGCTCTTCCAACAACC+TGG - chr5_3:60989592-60989611 None:intergenic 50.0%
!! AAGAGCACTTCCCACCGAAA+AGG + chr5_3:60989601-60989620 Msa0822570:CDS 50.0%
! GGGATGTTCATCCTTTTCGG+TGG - chr5_3:60989615-60989634 None:intergenic 50.0%
!! GAAGCTTTGGGCAACCAATG+AGG + chr5_3:60989649-60989668 Msa0822570:CDS 50.0%
GGATTTGGCACGAACCTCAT+TGG - chr5_3:60989666-60989685 None:intergenic 50.0%
GTGGAATCTAGGTGTGTACG+AGG + chr5_3:60990707-60990726 Msa0822570:intron 50.0%
TACTACTCTAAACGGCGCTG+TGG + chr5_3:60991396-60991415 Msa0822570:CDS 50.0%
ACGAGATGGCATCTCGTCAT+AGG + chr5_3:60991431-60991450 Msa0822570:CDS 50.0%
CGAGATGGCATCTCGTCATA+GGG + chr5_3:60991432-60991451 Msa0822570:CDS 50.0%
TGGCATCTCGTCATAGGGTC+AGG + chr5_3:60991437-60991456 Msa0822570:CDS 55.0%
! GCACAGATTAGCTGGGATGG+TGG - chr5_3:60991491-60991510 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 60989400 60992008 60989400 ID=Msa0822570;Name=Msa0822570
chr5_3 mRNA 60989400 60992008 60989400 ID=Msa0822570-mRNA-1;Parent=Msa0822570;Name=Msa0822570-mRNA-1;_AED=0.01;_eAED=0.01;_QI=68|1|1|1|1|1|4|369|178
chr5_3 exon 60989400 60989482 60989400 ID=Msa0822570-mRNA-1:exon:18751;Parent=Msa0822570-mRNA-1
chr5_3 exon 60989578 60989693 60989578 ID=Msa0822570-mRNA-1:exon:18752;Parent=Msa0822570-mRNA-1
chr5_3 exon 60989801 60989864 60989801 ID=Msa0822570-mRNA-1:exon:18753;Parent=Msa0822570-mRNA-1
chr5_3 exon 60991298 60992008 60991298 ID=Msa0822570-mRNA-1:exon:18754;Parent=Msa0822570-mRNA-1
chr5_3 five_prime_UTR 60989400 60989467 60989400 ID=Msa0822570-mRNA-1:five_prime_utr;Parent=Msa0822570-mRNA-1
chr5_3 CDS 60989468 60989482 60989468 ID=Msa0822570-mRNA-1:cds;Parent=Msa0822570-mRNA-1
chr5_3 CDS 60989578 60989693 60989578 ID=Msa0822570-mRNA-1:cds;Parent=Msa0822570-mRNA-1
chr5_3 CDS 60989801 60989864 60989801 ID=Msa0822570-mRNA-1:cds;Parent=Msa0822570-mRNA-1
chr5_3 CDS 60991298 60991639 60991298 ID=Msa0822570-mRNA-1:cds;Parent=Msa0822570-mRNA-1
chr5_3 three_prime_UTR 60991640 60992008 60991640 ID=Msa0822570-mRNA-1:three_prime_utr;Parent=Msa0822570-mRNA-1
Gene Sequence

>Msa0822570

ATGGTTAACGCTAAGTTTCACCAGTACCAGGTTGTTGGAAGAGCACTTCCCACCGAAAAGGATGAACATCCCAAGATTTATCGCATGAAGCTTTGGGCAACCAATGAGGTTCGTGCCAAATCCAAATTCTGGTATTTCTTGAGGAAGTTGAAGAAGGTTAAGAAAAGTAATGGACAAGTTTTGGCTATCAATGAGATCTTCGAGAAAAATCCTACCAAGATTAAGAACTATGGAATTTGGTTGCGTTATCAGAGTCGTACTGGTTATCACAATATGTACAAGGAATACCGTGATACTACTCTAAACGGCGCTGTGGAGACAATGTACAACGAGATGGCATCTCGTCATAGGGTCAGGTTTCCCTGCATTCAAATCATTAAGACTGCCACCATCCCAGCTAATCTGTGCAAAAGGGAGAGCACTAAGCAGTTTCACAATTCCAAAATCAAGTTCCCTCTGGTGTACAAGAAGATTAGGCCACCAACTAGGAGCCTAAAAACAACCTACAAAGCCAAGAAGCCAAACCTGTTTATGTGA
Protein sequence

>Msa0822570

MVNAKFHQYQVVGRALPTEKDEHPKIYRMKLWATNEVRAKSKFWYFLRKLKKVKKSNGQVLAINEIFEKNPTKIKNYGIWLRYQSRTGYHNMYKEYRDTTLNGAVETMYNEMASRHRVRFPCIQIIKTATIPANLCKRESTKQFHNSKIKFPLVYKKIRPPTRSLKTTYKAKKPNLFM*