AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0116130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116130 MtrunA17_Chr1g0212921 97.938 97 2 0 1 97 1 97 4.62e-62 184
Msa0116130 MtrunA17_Chr1g0182051 93.750 96 6 0 2 97 3 98 5.00e-58 174
Msa0116130 MtrunA17_Chr4g0030641 91.667 96 8 0 2 97 4 99 1.20e-55 168
Msa0116130 MtrunA17_Chr7g0249361 89.583 96 10 0 2 97 49 144 8.49e-55 167
Msa0116130 MtrunA17_Chr4g0036351 84.694 98 15 0 1 98 1 98 6.82e-52 159
Msa0116130 MtrunA17_Chr5g0419591 86.842 38 5 0 65 102 2 39 1.19e-16 67.4
Msa0116130 MtrunA17_Chr1g0206641 87.179 39 5 0 9 47 2 40 1.99e-15 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116130 AT2G39390.1 88.542 96 11 0 2 97 4 99 2.16e-54 166
Msa0116130 AT3G55170.4 85.714 98 14 0 2 99 4 101 3.84e-53 161
Msa0116130 AT3G55170.3 85.714 98 14 0 2 99 4 101 3.84e-53 161
Msa0116130 AT3G09500.1 86.598 97 13 0 2 98 4 100 5.99e-53 162
Msa0116130 AT5G02610.1 87.500 96 12 0 2 97 4 99 8.41e-53 161
Msa0116130 AT3G55170.5 85.567 97 14 0 2 98 4 100 5.30e-52 159
Msa0116130 AT3G55170.2 85.567 97 14 0 2 98 4 100 5.30e-52 159
Msa0116130 AT3G55170.1 85.567 97 14 0 2 98 4 100 5.30e-52 159
Msa0116130 AT5G02610.2 70.588 119 12 1 2 97 4 122 2.88e-47 148

Find 28 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATGAATTATACATTAAA+AGG 0.119721 1_3:+35000583 None:intergenic
CGAATGGCCCTGGTCTTCTT+TGG 0.171363 1_3:+35000637 None:intergenic
TCAAAGCAGCCTTCTGCTTC+TGG 0.210134 1_3:+35000704 None:intergenic
GAATGGCCCTGGTCTTCTTT+GGG 0.358511 1_3:+35000638 None:intergenic
GAAGCAGAAGGCTGCTTTGA+GGG 0.418273 1_3:-35000701 Msa0116130:CDS
CAAAGCAGCCTTCTGCTTCT+GGG 0.419055 1_3:+35000705 None:intergenic
GAGCCTTCTGCGAATGGCCC+TGG 0.419374 1_3:+35000627 None:intergenic
CTCCGTGTTGCTAAGGTCAC+TGG 0.447113 1_3:-35001079 Msa0116130:CDS
GTAAAGCAAGTTCAGCTTTG+AGG 0.452854 1_3:+35001101 None:intergenic
CATTAAAAGGGTTTAATACC+TGG 0.468129 1_3:+35000596 None:intergenic
ACATGAATTATACATTAAAA+GGG 0.470430 1_3:+35000584 None:intergenic
CTTGGTGAGCCTTCTGCGAA+TGG 0.472202 1_3:+35000621 None:intergenic
TGCTTTACTCCGTGTTGCTA+AGG 0.473685 1_3:-35001086 Msa0116130:CDS
AGGGTTTAATACCTGGTGCT+TGG 0.515451 1_3:+35000603 None:intergenic
ATCTCCGCCCAAAGAAGACC+AGG 0.531518 1_3:-35000645 Msa0116130:CDS
CAGAAGGCTCACCAAGCACC+AGG 0.535404 1_3:-35000614 Msa0116130:CDS
CTGCAGAAGAATTCAACAAT+GGG 0.536601 1_3:-35001280 Msa0116130:intron
TTCTTTGGGCGGAGATCAAG+TGG 0.553377 1_3:+35000652 None:intergenic
TAGTGAAGGTTCACGAGTTG+AGG 0.561491 1_3:-35001162 Msa0116130:CDS
GGTGAGGTTGTCGATTGCTC+AGG 0.570678 1_3:-35000743 Msa0116130:CDS
TGGCCCTGGTCTTCTTTGGG+CGG 0.571866 1_3:+35000641 None:intergenic
AGAAGCAGAAGGCTGCTTTG+AGG 0.576045 1_3:-35000702 Msa0116130:CDS
AGACCAGGGCCATTCGCAGA+AGG 0.591558 1_3:-35000630 Msa0116130:CDS
AGTGATTTCCCAGAAGCAGA+AGG 0.617415 1_3:-35000713 Msa0116130:CDS
CACCAGTGACCTTAGCAACA+CGG 0.624056 1_3:+35001077 None:intergenic
GCTGCAGAAGAATTCAACAA+TGG 0.630743 1_3:-35001281 Msa0116130:exon
CGTGTTGCTAAGGTCACTGG+TGG 0.692805 1_3:-35001076 Msa0116130:CDS
TCTCCGCCCAAAGAAGACCA+GGG 0.718890 1_3:-35000644 Msa0116130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATTTTTATTTACATTT+TGG + chr1_3:35000903-35000922 None:intergenic 10.0%
!!! TTGTTTTTGATTATTTTATT+TGG - chr1_3:35001155-35001174 Msa0116130:CDS 10.0%
!!! TGTTTTTGATTATTTTATTT+GGG - chr1_3:35001156-35001175 Msa0116130:CDS 10.0%
!!! TTGAATTTTTATTTACATTT+TGG + chr1_3:35000903-35000922 None:intergenic 10.0%
!!! TTGTTTTTGATTATTTTATT+TGG - chr1_3:35001155-35001174 Msa0116130:CDS 10.0%
!!! TGTTTTTGATTATTTTATTT+GGG - chr1_3:35001156-35001175 Msa0116130:CDS 10.0%
!!! TTGTTTGATTTCAGAAATTA+GGG + chr1_3:35000699-35000718 None:intergenic 20.0%
!!! TTTGTTTGATTTCAGAAATT+AGG + chr1_3:35000700-35000719 None:intergenic 20.0%
!!! CTTTATTCTTTTATGTCAAT+TGG - chr1_3:35000924-35000943 Msa0116130:intron 20.0%
!!! TTGTTTGATTTCAGAAATTA+GGG + chr1_3:35000699-35000718 None:intergenic 20.0%
!!! TTTGTTTGATTTCAGAAATT+AGG + chr1_3:35000700-35000719 None:intergenic 20.0%
!!! CTTTATTCTTTTATGTCAAT+TGG - chr1_3:35000924-35000943 Msa0116130:intron 20.0%
! AACAATTTATCCTTCAGAAT+TGG + chr1_3:35001037-35001056 None:intergenic 25.0%
! TGATTGTGTGATGATTATAA+TGG - chr1_3:35001076-35001095 Msa0116130:CDS 25.0%
! GATTGTGTGATGATTATAAT+GGG - chr1_3:35001077-35001096 Msa0116130:CDS 25.0%
! AACAATTTATCCTTCAGAAT+TGG + chr1_3:35001037-35001056 None:intergenic 25.0%
! TGATTGTGTGATGATTATAA+TGG - chr1_3:35001076-35001095 Msa0116130:CDS 25.0%
! GATTGTGTGATGATTATAAT+GGG - chr1_3:35001077-35001096 Msa0116130:CDS 25.0%
! TTTGATTTCAGAAATTAGGG+TGG + chr1_3:35000696-35000715 None:intergenic 30.0%
!!! TGTGTTTTGATTTGTAGTGA+AGG - chr1_3:35000760-35000779 Msa0116130:intron 30.0%
TATCCTTCAGAATTGGTAAA+TGG + chr1_3:35001030-35001049 None:intergenic 30.0%
GAAGGATAAATTGTTCCTTT+CGG - chr1_3:35001042-35001061 Msa0116130:intron 30.0%
!!! GTTTTTGTTTTGTGACTGTA+AGG - chr1_3:35001102-35001121 Msa0116130:CDS 30.0%
!! TTTATTTGGGTTTTGCAGTA+AGG - chr1_3:35001169-35001188 Msa0116130:intron 30.0%
CATTAAAAGGGTTTAATACC+TGG + chr1_3:35001343-35001362 None:intergenic 30.0%
! TTTGATTTCAGAAATTAGGG+TGG + chr1_3:35000696-35000715 None:intergenic 30.0%
!!! TGTGTTTTGATTTGTAGTGA+AGG - chr1_3:35000760-35000779 Msa0116130:intron 30.0%
TATCCTTCAGAATTGGTAAA+TGG + chr1_3:35001030-35001049 None:intergenic 30.0%
GAAGGATAAATTGTTCCTTT+CGG - chr1_3:35001042-35001061 Msa0116130:intron 30.0%
!!! GTTTTTGTTTTGTGACTGTA+AGG - chr1_3:35001102-35001121 Msa0116130:CDS 30.0%
!! TTTATTTGGGTTTTGCAGTA+AGG - chr1_3:35001169-35001188 Msa0116130:intron 30.0%
CATTAAAAGGGTTTAATACC+TGG + chr1_3:35001343-35001362 None:intergenic 30.0%
! TGATTTTGTAGTAAGCTGCT+AGG - chr1_3:35000593-35000612 Msa0116130:CDS 35.0%
! GATTTTGTAGTAAGCTGCTA+GGG - chr1_3:35000594-35000613 Msa0116130:CDS 35.0%
CTGCAGAAGAATTCAACAAT+GGG - chr1_3:35000656-35000675 Msa0116130:CDS 35.0%
TCAGAAATTAGGGTGGTTTA+GGG + chr1_3:35000689-35000708 None:intergenic 35.0%
TTCAGAAATTAGGGTGGTTT+AGG + chr1_3:35000690-35000709 None:intergenic 35.0%
GAAAAACAAAGCTGATCTGT+TGG - chr1_3:35000799-35000818 Msa0116130:intron 35.0%
!! TACATTTTGGAGAGCTTGTT+GGG + chr1_3:35000890-35000909 None:intergenic 35.0%
!! TTACATTTTGGAGAGCTTGT+TGG + chr1_3:35000891-35000910 None:intergenic 35.0%
!! AATTGGTGCTATGATCGTAA+AGG - chr1_3:35000941-35000960 Msa0116130:intron 35.0%
! AATTGTTCCTTTCGGTGTAA+TGG - chr1_3:35001050-35001069 Msa0116130:intron 35.0%
!!! TTTGTTTTGTGACTGTAAGG+TGG - chr1_3:35001105-35001124 Msa0116130:CDS 35.0%
! TGATTTTGTAGTAAGCTGCT+AGG - chr1_3:35000593-35000612 Msa0116130:CDS 35.0%
! GATTTTGTAGTAAGCTGCTA+GGG - chr1_3:35000594-35000613 Msa0116130:CDS 35.0%
CTGCAGAAGAATTCAACAAT+GGG - chr1_3:35000656-35000675 Msa0116130:CDS 35.0%
TCAGAAATTAGGGTGGTTTA+GGG + chr1_3:35000689-35000708 None:intergenic 35.0%
TTCAGAAATTAGGGTGGTTT+AGG + chr1_3:35000690-35000709 None:intergenic 35.0%
GAAAAACAAAGCTGATCTGT+TGG - chr1_3:35000799-35000818 Msa0116130:intron 35.0%
!! TACATTTTGGAGAGCTTGTT+GGG + chr1_3:35000890-35000909 None:intergenic 35.0%
!! TTACATTTTGGAGAGCTTGT+TGG + chr1_3:35000891-35000910 None:intergenic 35.0%
!! AATTGGTGCTATGATCGTAA+AGG - chr1_3:35000941-35000960 Msa0116130:intron 35.0%
! AATTGTTCCTTTCGGTGTAA+TGG - chr1_3:35001050-35001069 Msa0116130:intron 35.0%
!!! TTTGTTTTGTGACTGTAAGG+TGG - chr1_3:35001105-35001124 Msa0116130:CDS 35.0%
GCTGCAGAAGAATTCAACAA+TGG - chr1_3:35000655-35000674 Msa0116130:CDS 40.0%
GTAAAGCAAGTTCAGCTTTG+AGG + chr1_3:35000838-35000857 None:intergenic 40.0%
CGTCCATTTACCAATTCTGA+AGG - chr1_3:35001024-35001043 Msa0116130:intron 40.0%
ATCAAAGCCATTACACCGAA+AGG + chr1_3:35001060-35001079 None:intergenic 40.0%
!! ATTTGGGTTTTGCAGTAAGG+TGG - chr1_3:35001172-35001191 Msa0116130:intron 40.0%
GCTGCAGAAGAATTCAACAA+TGG - chr1_3:35000655-35000674 Msa0116130:CDS 40.0%
GTAAAGCAAGTTCAGCTTTG+AGG + chr1_3:35000838-35000857 None:intergenic 40.0%
CGTCCATTTACCAATTCTGA+AGG - chr1_3:35001024-35001043 Msa0116130:intron 40.0%
ATCAAAGCCATTACACCGAA+AGG + chr1_3:35001060-35001079 None:intergenic 40.0%
!! ATTTGGGTTTTGCAGTAAGG+TGG - chr1_3:35001172-35001191 Msa0116130:intron 40.0%
TAGTGAAGGTTCACGAGTTG+AGG - chr1_3:35000774-35000793 Msa0116130:intron 45.0%
! TGCTTTACTCCGTGTTGCTA+AGG - chr1_3:35000850-35000869 Msa0116130:intron 45.0%
AGTGATTTCCCAGAAGCAGA+AGG - chr1_3:35001223-35001242 Msa0116130:intron 45.0%
AGGGTTTAATACCTGGTGCT+TGG + chr1_3:35001336-35001355 None:intergenic 45.0%
TAGTGAAGGTTCACGAGTTG+AGG - chr1_3:35000774-35000793 Msa0116130:intron 45.0%
! TGCTTTACTCCGTGTTGCTA+AGG - chr1_3:35000850-35000869 Msa0116130:intron 45.0%
AGTGATTTCCCAGAAGCAGA+AGG - chr1_3:35001223-35001242 Msa0116130:intron 45.0%
AGGGTTTAATACCTGGTGCT+TGG + chr1_3:35001336-35001355 None:intergenic 45.0%
CACCAGTGACCTTAGCAACA+CGG + chr1_3:35000862-35000881 None:intergenic 50.0%
! GGTTTTGCAGTAAGGTGGTG+AGG - chr1_3:35001177-35001196 Msa0116130:intron 50.0%
CAAAGCAGCCTTCTGCTTCT+GGG + chr1_3:35001234-35001253 None:intergenic 50.0%
TCAAAGCAGCCTTCTGCTTC+TGG + chr1_3:35001235-35001254 None:intergenic 50.0%
! AGAAGCAGAAGGCTGCTTTG+AGG - chr1_3:35001234-35001253 Msa0116130:intron 50.0%
! GAAGCAGAAGGCTGCTTTGA+GGG - chr1_3:35001235-35001254 Msa0116130:intron 50.0%
TTCTTTGGGCGGAGATCAAG+TGG + chr1_3:35001287-35001306 None:intergenic 50.0%
! GAATGGCCCTGGTCTTCTTT+GGG + chr1_3:35001301-35001320 None:intergenic 50.0%
CACCAGTGACCTTAGCAACA+CGG + chr1_3:35000862-35000881 None:intergenic 50.0%
! GGTTTTGCAGTAAGGTGGTG+AGG - chr1_3:35001177-35001196 Msa0116130:intron 50.0%
CAAAGCAGCCTTCTGCTTCT+GGG + chr1_3:35001234-35001253 None:intergenic 50.0%
TCAAAGCAGCCTTCTGCTTC+TGG + chr1_3:35001235-35001254 None:intergenic 50.0%
! AGAAGCAGAAGGCTGCTTTG+AGG - chr1_3:35001234-35001253 Msa0116130:intron 50.0%
! GAAGCAGAAGGCTGCTTTGA+GGG - chr1_3:35001235-35001254 Msa0116130:intron 50.0%
TTCTTTGGGCGGAGATCAAG+TGG + chr1_3:35001287-35001306 None:intergenic 50.0%
! GAATGGCCCTGGTCTTCTTT+GGG + chr1_3:35001301-35001320 None:intergenic 50.0%
CACACACACACACACGCAAG+AGG - chr1_3:35000625-35000644 Msa0116130:CDS 55.0%
! CTCCGTGTTGCTAAGGTCAC+TGG - chr1_3:35000857-35000876 Msa0116130:intron 55.0%
! CGTGTTGCTAAGGTCACTGG+TGG - chr1_3:35000860-35000879 Msa0116130:intron 55.0%
! GGTGAGGTTGTCGATTGCTC+AGG - chr1_3:35001193-35001212 Msa0116130:intron 55.0%
ATCTCCGCCCAAAGAAGACC+AGG - chr1_3:35001291-35001310 Msa0116130:five_prime_UTR 55.0%
TCTCCGCCCAAAGAAGACCA+GGG - chr1_3:35001292-35001311 Msa0116130:five_prime_UTR 55.0%
! CGAATGGCCCTGGTCTTCTT+TGG + chr1_3:35001302-35001321 None:intergenic 55.0%
CTTGGTGAGCCTTCTGCGAA+TGG + chr1_3:35001318-35001337 None:intergenic 55.0%
CACACACACACACACGCAAG+AGG - chr1_3:35000625-35000644 Msa0116130:CDS 55.0%
! CTCCGTGTTGCTAAGGTCAC+TGG - chr1_3:35000857-35000876 Msa0116130:intron 55.0%
! CGTGTTGCTAAGGTCACTGG+TGG - chr1_3:35000860-35000879 Msa0116130:intron 55.0%
! GGTGAGGTTGTCGATTGCTC+AGG - chr1_3:35001193-35001212 Msa0116130:intron 55.0%
ATCTCCGCCCAAAGAAGACC+AGG - chr1_3:35001291-35001310 Msa0116130:five_prime_UTR 55.0%
TCTCCGCCCAAAGAAGACCA+GGG - chr1_3:35001292-35001311 Msa0116130:five_prime_UTR 55.0%
! CGAATGGCCCTGGTCTTCTT+TGG + chr1_3:35001302-35001321 None:intergenic 55.0%
CTTGGTGAGCCTTCTGCGAA+TGG + chr1_3:35001318-35001337 None:intergenic 55.0%
! TGGCCCTGGTCTTCTTTGGG+CGG + chr1_3:35001298-35001317 None:intergenic 60.0%
AGACCAGGGCCATTCGCAGA+AGG - chr1_3:35001306-35001325 Msa0116130:five_prime_UTR 60.0%
CAGAAGGCTCACCAAGCACC+AGG - chr1_3:35001322-35001341 Msa0116130:five_prime_UTR 60.0%
! TGGCCCTGGTCTTCTTTGGG+CGG + chr1_3:35001298-35001317 None:intergenic 60.0%
AGACCAGGGCCATTCGCAGA+AGG - chr1_3:35001306-35001325 Msa0116130:five_prime_UTR 60.0%
CAGAAGGCTCACCAAGCACC+AGG - chr1_3:35001322-35001341 Msa0116130:five_prime_UTR 60.0%
GAGCCTTCTGCGAATGGCCC+TGG + chr1_3:35001312-35001331 None:intergenic 65.0%
GAGCCTTCTGCGAATGGCCC+TGG + chr1_3:35001312-35001331 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 35000591 35001367 35000591 ID=Msa0116130;Name=Msa0116130
chr1_3 mRNA 35000591 35001367 35000591 ID=Msa0116130-mRNA-1;Parent=Msa0116130;Name=Msa0116130-mRNA-1;_AED=0.02;_eAED=0.02;_QI=83|1|0.66|1|1|1|3|0|104
chr1_3 exon 35000591 35000771 35000591 ID=Msa0116130-mRNA-1:exon:11192;Parent=Msa0116130-mRNA-1
chr1_3 exon 35001052 35001181 35001052 ID=Msa0116130-mRNA-1:exon:11191;Parent=Msa0116130-mRNA-1
chr1_3 exon 35001281 35001367 35001281 ID=Msa0116130-mRNA-1:exon:11190;Parent=Msa0116130-mRNA-1
chr1_3 five_prime_UTR 35001285 35001367 35001285 ID=Msa0116130-mRNA-1:five_prime_utr;Parent=Msa0116130-mRNA-1
chr1_3 CDS 35001281 35001284 35001281 ID=Msa0116130-mRNA-1:cds;Parent=Msa0116130-mRNA-1
chr1_3 CDS 35001052 35001181 35001052 ID=Msa0116130-mRNA-1:cds;Parent=Msa0116130-mRNA-1
chr1_3 CDS 35000591 35000771 35000591 ID=Msa0116130-mRNA-1:cds;Parent=Msa0116130-mRNA-1
Gene Sequence

>Msa0116130

ATGGTGAAGGTTCACGAGTTGAGGCAGAAAAACAAAGCTGATCTGTTGGCTCAGCTCAAAGACCTCAAAGCTGAACTTGCTTTACTCCGTGTTGCTAAGGTCACTGGTGGTGCACCCAACAAGCTCTCCAAAATTAAGGTGGTGAGGTTGTCGATTGCTCAGGTGTTGACAGTGATTTCCCAGAAGCAGAAGGCTGCTTTGAGGGAAGTTTACAAGAACAAGAAGTATTTGCCACTTGATCTCCGCCCAAAGAAGACCAGGGCCATTCGCAGAAGGCTCACCAAGCACCAGGTATTAAACCCTTTTAATGTATAA

Protein sequence

>Msa0116130

MVKVHELRQKNKADLLAQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQVLNPFNV*