AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0823560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823560 MtrunA17_Chr5g0444201 99.091 110 1 0 34 143 1 110 1.98e-78 227
Msa0823560 MtrunA17_Chr3g0098971 84.404 109 17 0 35 143 4 112 3.34e-65 194
Msa0823560 MtrunA17_Chr4g0024171 71.560 109 30 1 36 143 5 113 2.11e-55 169
Msa0823560 MtrunA17_Chr2g0319561 72.072 111 29 1 35 143 4 114 7.26e-55 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0823560 AT5G54940.1 81.651 109 20 0 35 143 4 112 4.72e-64 191
Msa0823560 AT5G54940.3 81.651 109 20 0 35 143 4 112 4.72e-64 191
Msa0823560 AT5G54940.2 81.651 109 20 0 35 143 4 112 4.72e-64 191
Msa0823560 AT1G54290.1 72.727 110 29 1 35 143 4 113 1.45e-57 175
Msa0823560 AT4G27130.1 73.636 110 28 1 35 143 4 113 1.99e-57 174
Msa0823560 AT5G54760.2 73.636 110 28 1 35 143 4 113 3.12e-57 174
Msa0823560 AT5G54760.4 73.636 110 28 1 35 143 4 113 3.12e-57 174
Msa0823560 AT5G54760.3 73.636 110 28 1 35 143 4 113 3.12e-57 174
Msa0823560 AT5G54760.1 73.636 110 28 1 35 143 4 113 3.12e-57 174

Find 29 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTTCTTGAGATCTTTA+AGG 0.098306 5_3:+62239587 None:intergenic
CAAAAGAGTATGTGCATATT+AGG 0.278072 5_3:-62239684 Msa0823560:CDS
TCAGTCCTTGCACTGTTGTT+AGG 0.278405 5_3:+62239635 None:intergenic
TAGAAGAGAGTTGAAGAGAT+TGG 0.333969 5_3:+62240800 None:intergenic
AAGGACAACATCAAGATTCA+TGG 0.385779 5_3:-62239451 Msa0823560:CDS
AGAAGAGAGTTGAAGAGATT+GGG 0.422313 5_3:+62240801 None:intergenic
TCATGCTGGTCTTGCTAGAA+AGG 0.426864 5_3:-62239470 Msa0823560:CDS
AGTTGAAGAGATTGGGAATG+TGG 0.436578 5_3:+62240808 None:intergenic
TATCTCTATCTGCAGACTAC+AGG 0.445137 5_3:-62240759 Msa0823560:intron
TGCGTTGATCACCTTGGAGT+TGG 0.445951 5_3:+62239515 None:intergenic
GATTCTTTAGCCTCAGCAAA+TGG 0.452308 5_3:+62239721 None:intergenic
TCTCACTTCCTCATTCATGC+TGG 0.461083 5_3:-62239484 Msa0823560:CDS
GTTGTGAATTGCAAGGATCT+TGG 0.473894 5_3:-62239547 Msa0823560:CDS
GCAAATGGATCAAATGTGCT+TGG 0.480564 5_3:+62239736 None:intergenic
AGAGATTGGGAATGTGGAGG+TGG 0.498637 5_3:+62240814 None:intergenic
CATTCTTGCGTTGATCACCT+TGG 0.510216 5_3:+62239509 None:intergenic
CAAATGGATCAAATGTGCTT+GGG 0.511747 5_3:+62239737 None:intergenic
CACATTTGATCCATTTGCTG+AGG 0.553489 5_3:-62239731 Msa0823560:CDS
AACAGTGCAAGGACTGAAGA+AGG 0.557278 5_3:-62239629 Msa0823560:CDS
ATTAGGATACAACAAAGGAA+TGG 0.567289 5_3:-62239667 Msa0823560:CDS
GAGATTGGGAATGTGGAGGT+GGG 0.587196 5_3:+62240815 None:intergenic
TAGCAAGACCAGCATGAATG+AGG 0.588886 5_3:+62239476 None:intergenic
AATCACAAAGAAAGTGTACA+TGG 0.596673 5_3:-62239773 Msa0823560:CDS
GGCAAGATTATCCAACTCCA+AGG 0.618688 5_3:-62239526 Msa0823560:CDS
AAGAGCCTAACAACAGTGCA+AGG 0.631107 5_3:-62239640 Msa0823560:CDS
GCTAAAGAATCAGATGCACC+AGG 0.655042 5_3:-62239709 Msa0823560:CDS
TGGAAATGTTGTGAATTGCA+AGG 0.663364 5_3:-62239554 Msa0823560:CDS
TGAAGAGATTGGGAATGTGG+AGG 0.681590 5_3:+62240811 None:intergenic
TGCATATTAGGATACAACAA+AGG 0.686189 5_3:-62239672 Msa0823560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTTTAAAACTAATT+TGG + chr5_3:62240222-62240241 None:intergenic 10.0%
!!! ATTAGTTTTAAAATAAAACT+AGG - chr5_3:62240223-62240242 Msa0823560:intron 10.0%
!! TGATATATATAATTCAAAAT+TGG + chr5_3:62240312-62240331 None:intergenic 10.0%
!!! GTTTTATTTTAAAACTAATT+TGG + chr5_3:62240222-62240241 None:intergenic 10.0%
!!! ATTAGTTTTAAAATAAAACT+AGG - chr5_3:62240223-62240242 Msa0823560:intron 10.0%
!! TGATATATATAATTCAAAAT+TGG + chr5_3:62240312-62240331 None:intergenic 10.0%
!! ATATATATCATATGAATGAA+TGG - chr5_3:62240322-62240341 Msa0823560:intron 15.0%
!!! AAACATAATCTTTAAAACTA+AGG - chr5_3:62240363-62240382 Msa0823560:intron 15.0%
!! ATATATATCATATGAATGAA+TGG - chr5_3:62240322-62240341 Msa0823560:intron 15.0%
!!! AAACATAATCTTTAAAACTA+AGG - chr5_3:62240363-62240382 Msa0823560:intron 15.0%
!!! AATATACTCCAAGTTTTATA+TGG - chr5_3:62239695-62239714 Msa0823560:CDS 20.0%
!! TATATTCACCATATAAAACT+TGG + chr5_3:62239706-62239725 None:intergenic 20.0%
!! AGAAAAAAAGTTATGAAGAA+GGG + chr5_3:62239884-62239903 None:intergenic 20.0%
!! AAGAAAAAAAGTTATGAAGA+AGG + chr5_3:62239885-62239904 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_3:62240114-62240133 None:intergenic 20.0%
!!! TTAATCTGATTTTAATATCC+TGG - chr5_3:62240403-62240422 Msa0823560:intron 20.0%
!!! AATATACTCCAAGTTTTATA+TGG - chr5_3:62239695-62239714 Msa0823560:CDS 20.0%
!! TATATTCACCATATAAAACT+TGG + chr5_3:62239706-62239725 None:intergenic 20.0%
!! AGAAAAAAAGTTATGAAGAA+GGG + chr5_3:62239884-62239903 None:intergenic 20.0%
!! AAGAAAAAAAGTTATGAAGA+AGG + chr5_3:62239885-62239904 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_3:62240114-62240133 None:intergenic 20.0%
!!! TTAATCTGATTTTAATATCC+TGG - chr5_3:62240403-62240422 Msa0823560:intron 20.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_3:62239604-62239623 Msa0823560:CDS 25.0%
! ATATTTACAATTGTTCCACA+TGG + chr5_3:62239830-62239849 None:intergenic 25.0%
! TAAAACTAGGAATATATGAG+TGG - chr5_3:62240236-62240255 Msa0823560:intron 25.0%
! TATTCACTAATTTCAAACCT+CGG + chr5_3:62240281-62240300 None:intergenic 25.0%
! ATATCATATGAATGAATGGT+TGG - chr5_3:62240326-62240345 Msa0823560:intron 25.0%
! TCAGATTAAAACCAAATCAA+AGG + chr5_3:62240392-62240411 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_3:62240448-62240467 None:intergenic 25.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_3:62239604-62239623 Msa0823560:CDS 25.0%
! ATATTTACAATTGTTCCACA+TGG + chr5_3:62239830-62239849 None:intergenic 25.0%
! TAAAACTAGGAATATATGAG+TGG - chr5_3:62240236-62240255 Msa0823560:intron 25.0%
! TATTCACTAATTTCAAACCT+CGG + chr5_3:62240281-62240300 None:intergenic 25.0%
! ATATCATATGAATGAATGGT+TGG - chr5_3:62240326-62240345 Msa0823560:intron 25.0%
! TCAGATTAAAACCAAATCAA+AGG + chr5_3:62240392-62240411 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_3:62240448-62240467 None:intergenic 25.0%
TAGAATCACCATATCAAACT+TGG + chr5_3:62239615-62239634 None:intergenic 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_3:62239789-62239808 Msa0823560:intron 30.0%
AATTCAAACATAGCTCCTTA+AGG + chr5_3:62239807-62239826 None:intergenic 30.0%
TGTGCAAGTTAGAAGATAAA+TGG + chr5_3:62240066-62240085 None:intergenic 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_3:62240486-62240505 Msa0823560:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_3:62240575-62240594 Msa0823560:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_3:62240587-62240606 Msa0823560:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_3:62240592-62240611 Msa0823560:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_3:62240675-62240694 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_3:62240685-62240704 Msa0823560:intron 30.0%
TAGAATCACCATATCAAACT+TGG + chr5_3:62239615-62239634 None:intergenic 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_3:62239789-62239808 Msa0823560:intron 30.0%
AATTCAAACATAGCTCCTTA+AGG + chr5_3:62239807-62239826 None:intergenic 30.0%
TGTGCAAGTTAGAAGATAAA+TGG + chr5_3:62240066-62240085 None:intergenic 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_3:62240486-62240505 Msa0823560:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_3:62240575-62240594 Msa0823560:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_3:62240587-62240606 Msa0823560:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_3:62240592-62240611 Msa0823560:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_3:62240675-62240694 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_3:62240685-62240704 Msa0823560:intron 30.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_3:62239461-62239480 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_3:62239462-62239481 None:intergenic 35.0%
TCTTTCCAAGTTTCGACAAT+AGG - chr5_3:62239757-62239776 Msa0823560:CDS 35.0%
TTCAACCTATTGTCGAAACT+TGG + chr5_3:62239765-62239784 None:intergenic 35.0%
AGCTATGTTTGAATTCCATG+TGG - chr5_3:62239812-62239831 Msa0823560:intron 35.0%
AACTAAGGAGACCTTTGATT+TGG - chr5_3:62240378-62240397 Msa0823560:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_3:62240525-62240544 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_3:62240705-62240724 Msa0823560:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_3:62240808-62240827 Msa0823560:CDS 35.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_3:62239461-62239480 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_3:62239462-62239481 None:intergenic 35.0%
TCTTTCCAAGTTTCGACAAT+AGG - chr5_3:62239757-62239776 Msa0823560:CDS 35.0%
TTCAACCTATTGTCGAAACT+TGG + chr5_3:62239765-62239784 None:intergenic 35.0%
AGCTATGTTTGAATTCCATG+TGG - chr5_3:62239812-62239831 Msa0823560:intron 35.0%
AACTAAGGAGACCTTTGATT+TGG - chr5_3:62240378-62240397 Msa0823560:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_3:62240525-62240544 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_3:62240705-62240724 Msa0823560:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_3:62240808-62240827 Msa0823560:CDS 35.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_3:62239454-62239473 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_3:62239500-62239519 Msa0823560:CDS 40.0%
TCTCTTGATTGCAAGGATCT+AGG + chr5_3:62240130-62240149 None:intergenic 40.0%
GTGTACTTCTCTTGATTGCA+AGG + chr5_3:62240137-62240156 None:intergenic 40.0%
CGATCTTCATTTAACAACCG+AGG - chr5_3:62240261-62240280 Msa0823560:intron 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_3:62240432-62240451 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_3:62240433-62240452 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_3:62240434-62240453 Msa0823560:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_3:62240526-62240545 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_3:62240528-62240547 Msa0823560:intron 40.0%
GATTCTTTAGCCTCAGCAAA+TGG + chr5_3:62240541-62240560 None:intergenic 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_3:62240712-62240731 Msa0823560:intron 40.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_3:62239454-62239473 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_3:62239500-62239519 Msa0823560:CDS 40.0%
TCTCTTGATTGCAAGGATCT+AGG + chr5_3:62240130-62240149 None:intergenic 40.0%
GTGTACTTCTCTTGATTGCA+AGG + chr5_3:62240137-62240156 None:intergenic 40.0%
CGATCTTCATTTAACAACCG+AGG - chr5_3:62240261-62240280 Msa0823560:intron 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_3:62240432-62240451 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_3:62240433-62240452 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_3:62240434-62240453 Msa0823560:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_3:62240526-62240545 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_3:62240528-62240547 Msa0823560:intron 40.0%
GATTCTTTAGCCTCAGCAAA+TGG + chr5_3:62240541-62240560 None:intergenic 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_3:62240712-62240731 Msa0823560:intron 40.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_3:62239451-62239470 None:intergenic 45.0%
TTAAGGTGCATGCATGCATG+GGG + chr5_3:62240431-62240450 None:intergenic 45.0%
GCTAAAGAATCAGATGCACC+AGG - chr5_3:62240550-62240569 Msa0823560:intron 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_3:62240571-62240590 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_3:62240619-62240638 Msa0823560:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_3:62240627-62240646 None:intergenic 45.0%
AACAGTGCAAGGACTGAAGA+AGG - chr5_3:62240630-62240649 Msa0823560:intron 45.0%
GGCAAGATTATCCAACTCCA+AGG - chr5_3:62240733-62240752 Msa0823560:intron 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_3:62240753-62240772 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_3:62240775-62240794 Msa0823560:CDS 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_3:62240786-62240805 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_3:62240789-62240808 Msa0823560:CDS 45.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_3:62239451-62239470 None:intergenic 45.0%
TTAAGGTGCATGCATGCATG+GGG + chr5_3:62240431-62240450 None:intergenic 45.0%
GCTAAAGAATCAGATGCACC+AGG - chr5_3:62240550-62240569 Msa0823560:intron 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_3:62240571-62240590 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_3:62240619-62240638 Msa0823560:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_3:62240627-62240646 None:intergenic 45.0%
AACAGTGCAAGGACTGAAGA+AGG - chr5_3:62240630-62240649 Msa0823560:intron 45.0%
GGCAAGATTATCCAACTCCA+AGG - chr5_3:62240733-62240752 Msa0823560:intron 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_3:62240753-62240772 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_3:62240775-62240794 Msa0823560:CDS 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_3:62240786-62240805 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_3:62240789-62240808 Msa0823560:CDS 45.0%
!!! ATTTTAACAATTATATTTTT+TGG - chr5_3:62239993-62240012 Msa0823560:intron 5.0%
!!! ATTTTAACAATTATATTTTT+TGG - chr5_3:62239993-62240012 Msa0823560:intron 5.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_3:62239447-62239466 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_3:62239448-62239467 None:intergenic 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_3:62240747-62240766 None:intergenic 50.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_3:62239447-62239466 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_3:62239448-62239467 None:intergenic 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_3:62240747-62240766 None:intergenic 50.0%
GCATGCATGCATGGGGATCC+AGG + chr5_3:62240424-62240443 None:intergenic 60.0%
GCATGCATGCATGGGGATCC+AGG + chr5_3:62240424-62240443 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 62239444 62240837 62239444 ID=Msa0823560;Name=Msa0823560
chr5_3 mRNA 62239444 62240837 62239444 ID=Msa0823560-mRNA-1;Parent=Msa0823560;Name=Msa0823560-mRNA-1;_AED=0.15;_eAED=0.20;_QI=0|0|0.5|1|0|0.5|2|0|143
chr5_3 exon 62239444 62239797 62239444 ID=Msa0823560-mRNA-1:exon:19369;Parent=Msa0823560-mRNA-1
chr5_3 exon 62240760 62240837 62240760 ID=Msa0823560-mRNA-1:exon:19368;Parent=Msa0823560-mRNA-1
chr5_3 CDS 62240760 62240837 62240760 ID=Msa0823560-mRNA-1:cds;Parent=Msa0823560-mRNA-1
chr5_3 CDS 62239444 62239797 62239444 ID=Msa0823560-mRNA-1:cds;Parent=Msa0823560-mRNA-1
Gene Sequence

>Msa0823560

CCCACCTCCACATTCCCAATCTCTTCAACTCTCTTCTATTTGTCTTCTAGCTTTTCTATCTCTATCTGCAGACTACAGCTAATCACAAAGAAAGTGTACATGGTTGATATTCAGATCCCAAGCACATTTGATCCATTTGCTGAGGCTAAAGAATCAGATGCACCAGGTGCAAAAGAGTATGTGCATATTAGGATACAACAAAGGAATGGAAAAAAGAGCCTAACAACAGTGCAAGGACTGAAGAAGGAGTTCAGCTACGAGAAGATCCTTAAAGATCTCAAGAAAGAGTTTTGTTGCAATGGAAATGTTGTGAATTGCAAGGATCTTGGCAAGATTATCCAACTCCAAGGTGATCAACGCAAGAATGTTTCTCACTTCCTCATTCATGCTGGTCTTGCTAGAAAGGACAACATCAAGATTCATGGTTTTTAA

Protein sequence

>Msa0823560

PTSTFPISSTLFYLSSSFSISICRLQLITKKVYMVDIQIPSTFDPFAEAKESDAPGAKEYVHIRIQQRNGKKSLTTVQGLKKEFSYEKILKDLKKEFCCNGNVVNCKDLGKIIQLQGDQRKNVSHFLIHAGLARKDNIKIHGF*