AlfalfaGEDB | Msa0253370

M. sativa cultivar zhongmu-4 / Msa0253370

KEGG: K00799
GO: -
PFAM: PF02798.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0253370	RDX68874.1	70.681	191	45	1	130	309	204	394	8.16e-88	286
Msa0253370	RDX68874.1	71.084	415	87	2	118	521	1	393	0.0	608
Msa0253370	RDX68874.1	75.269	93	23	0	13	105	204	296	1.50e-36	151
Msa0253370	RDX68874.1	80.000	105	21	0	1	105	1	105	5.36e-46	177

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0253370	sp\|P46417\|GSTX3_SOYBN	80.556	216	42	0	320	535	1	216	1.83e-117	348
Msa0253370	sp\|P46417\|GSTX3_SOYBN	75.238	210	41	1	118	316	1	210	1.09e-103	313
Msa0253370	sp\|P46417\|GSTX3_SOYBN	84.762	105	16	0	1	105	1	105	2.73e-57	193

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0253370	A0A371ES94	70.681	191	45	1	130	309	204	394	3.90e-88	286
Msa0253370	A0A371ES94	71.084	415	87	2	118	521	1	393	0.0	608
Msa0253370	A0A371ES94	75.269	93	23	0	13	105	204	296	7.15e-37	151
Msa0253370	A0A371ES94	80.000	105	21	0	1	105	1	105	2.56e-46	177

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004350	Msa0253370	0.835337	1.779411e-56	-8.615850e-47
Msa0005350	Msa0253370	0.806300	8.797255e-50	-8.615850e-47
Msa0006700	Msa0253370	0.896772	2.676726e-76	-8.615850e-47
Msa0007230	Msa0253370	0.868183	7.889882e-66	-8.615850e-47
Msa0008330	Msa0253370	0.837647	4.593805e-57	-8.615850e-47
Msa0008850	Msa0253370	0.857553	1.509274e-62	-8.615850e-47
Msa0011720	Msa0253370	0.863983	1.685755e-64	-8.615850e-47
Msa0011850	Msa0253370	0.810329	1.216821e-50	-8.615850e-47
Msa0012740	Msa0253370	0.839323	1.697581e-57	-8.615850e-47
Msa0012780	Msa0253370	-0.809004	2.344192e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0253370	MtrunA17_Chr2g0312691	95.434	219	10	0	320	538	1	219	1.30e-143	412
Msa0253370	MtrunA17_Chr2g0312691	78.673	211	34	1	118	317	1	211	6.22e-110	326
Msa0253370	MtrunA17_Chr2g0312691	88.571	105	12	0	1	105	1	105	5.17e-61	200
Msa0253370	MtrunA17_Chr2g0312681	89.041	219	24	0	320	538	1	219	1.02e-142	410
Msa0253370	MtrunA17_Chr2g0312681	90.521	211	9	1	118	317	1	211	6.84e-136	392
Msa0253370	MtrunA17_Chr2g0312681	97.143	105	3	0	1	105	1	105	3.53e-66	214
Msa0253370	MtrunA17_Chr2g0312711	88.584	219	24	1	320	538	1	218	2.92e-131	380
Msa0253370	MtrunA17_Chr2g0312711	77.251	211	36	2	118	317	1	210	2.23e-107	320
Msa0253370	MtrunA17_Chr2g0312711	88.991	109	12	0	1	109	1	109	1.13e-61	202
Msa0253370	MtrunA17_Chr2g0312771	66.210	219	74	0	320	538	1	219	3.13e-109	325

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0253370	AT1G17180.1	64.840	219	77	0	320	538	1	219	9.10e-106	316
Msa0253370	AT1G17180.1	60.287	209	72	1	118	315	1	209	2.41e-88	271
Msa0253370	AT1G17180.1	64.286	112	40	0	1	112	1	112	9.86e-46	160
Msa0253370	AT1G78380.1	67.130	216	71	0	320	535	1	216	8.52e-105	313
Msa0253370	AT1G78380.1	63.158	209	66	1	118	315	1	209	8.71e-90	275
Msa0253370	AT1G78380.1	76.190	105	25	0	1	105	1	105	4.94e-49	169
Msa0253370	AT1G78370.1	63.256	215	79	0	320	534	1	215	1.47e-98	297
Msa0253370	AT1G78370.1	61.165	206	69	1	118	312	1	206	2.16e-85	264
Msa0253370	AT1G78370.1	69.524	105	32	0	1	105	1	105	2.07e-46	162
Msa0253370	AT1G78360.1	63.636	220	79	1	320	538	1	220	1.27e-97	295

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Find 98 sgRNAs with CRISPR-Local

Find 653 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGAAGAATATGAGGA+AGG	0.489666	2_2:+45179482	Msa0253370:CDS
AAAGGTGAAGAGCATGAGGA+AGG	0.578570	2_2:+45183031	Msa0253370:CDS
AAAGTGGCACCACCGTAAAA+AGG	0.374250	2_2:-45183109	None:intergenic
AACTCTTCCTGATCAAATGA+AGG	0.495473	2_2:+45183273	Msa0253370:CDS
AAGAAAATGTGGCTTTCAAA+AGG	0.435917	2_2:+45179464	Msa0253370:CDS
AAGACACTAGAGGAAACTTT+AGG	0.341946	2_2:+45183079	Msa0253370:CDS
AAGGAGAAGAATATGAGGAA+GGG	0.539682	2_2:+45179483	Msa0253370:CDS
AAGGTGAAGAGCATGAGGAA+GGG	0.585110	2_2:+45183032	Msa0253370:CDS
AATAATTGCAGATTCACAAA+TGG	0.471536	2_2:-45173512	None:intergenic
AATAATTGCAGATTCACAAA+TGG	0.471536	2_2:-45178617	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGTAATTCTGTTAGATACA+TGG	+	chr2_2:45173335-45173354	Msa0253370:CDS	30.0%
GGTAATTCTGTTAGATACAT+GGG	+	chr2_2:45173336-45173355	Msa0253370:CDS	30.0%
ACTGGAATCTTCTTATGAAT+TGG	-	chr2_2:45173472-45173491	None:intergenic	30.0%
ATTCCAGTTCTTATCCATAA+TGG	+	chr2_2:45173484-45173503	Msa0253370:CDS	30.0%
TTACCATTATGGATAAGAAC+TGG	-	chr2_2:45173490-45173509	None:intergenic	30.0%
TGCAATACATAGATGAAGTT+TGG	+	chr2_2:45173536-45173555	Msa0253370:CDS	30.0%
GGTTGATTATAGTGACAAAA+AGG	+	chr2_2:45173618-45173637	Msa0253370:CDS	30.0%
ATCAATCATTCCCTTAACTT+TGG	-	chr2_2:45173835-45173854	None:intergenic	30.0%
TAGAAGAATATAGATGAAGG+TGG	-	chr2_2:45174656-45174675	None:intergenic	30.0%
TCCTTATTATGTCATTGAGA+CGG	+	chr2_2:45174830-45174849	Msa0253370:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	45173325	45183609	45173325	ID=Msa0253370;Name=Msa0253370
chr2_2	mRNA	45173325	45183609	45173325	ID=Msa0253370-mRNA-1;Parent=Msa0253370;Name=Msa0253370-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0.2\|0.16\|1\|0.6\|0.33\|6\|267\|538
chr2_2	exon	45173325	45173639	45173325	ID=Msa0253370-mRNA-1:exon:13917;Parent=Msa0253370-mRNA-1
chr2_2	exon	45178394	45178744	45178394	ID=Msa0253370-mRNA-1:exon:13918;Parent=Msa0253370-mRNA-1
chr2_2	exon	45179449	45179585	45179449	ID=Msa0253370-mRNA-1:exon:13919;Parent=Msa0253370-mRNA-1
chr2_2	exon	45179663	45179791	45179663	ID=Msa0253370-mRNA-1:exon:13920;Parent=Msa0253370-mRNA-1
chr2_2	exon	45182084	45182423	45182084	ID=Msa0253370-mRNA-1:exon:13921;Parent=Msa0253370-mRNA-1
chr2_2	exon	45182998	45183609	45182998	ID=Msa0253370-mRNA-1:exon:13922;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45173325	45173639	45173325	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45178394	45178744	45178394	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45179449	45179585	45179449	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45179663	45179791	45179663	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45182084	45182423	45182084	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	CDS	45182998	45183342	45182998	ID=Msa0253370-mRNA-1:cds;Parent=Msa0253370-mRNA-1
chr2_2	three_prime_UTR	45183343	45183609	45183343	ID=Msa0253370-mRNA-1:three_prime_utr;Parent=Msa0253370-mRNA-1

Gene Sequence

>Msa0253370

ATGGCGAACGAGGTAATTCTGTTAGATACATGGGCTAGCATGTTTGGTATGAGAGCAAGAATTGCATTAGCTGAAAAGAGTGTTAAATATGAGTATAAAGAAGAAAATCTTAGAAATAAGAGTCCTTTACTTCTTCAGATGAATCCAATTCATAAGAAGATTCCAGTTCTTATCCATAATGGTAAATCCATTTGTGAATCTGCAATTATTGTGCAATACATAGATGAAGTTTGGAATGATAAAGCTTCATTCATGCCTTCTAATCCTTATGAGAGAGCTCAAGCTAGATTTTGGGTTGATTATAGTGACAAAAAGCCAGTTCTCACTAGACAACTTAGGATTTTTACAATCATGGCGAACGAGGTAATTCTGTTAGATACATGGGCTAGCATGTTTGGTATGAGAGCAAGAATTGCATTAGCTGAAAAGAGTGTCAAATATGAGTACAAAGAAGAAGATCTTAGAAATAAGAGTCCTTTACTTCTTCAGATGAATCCAATTCATAAGAAGATTCCAGTTCTTATCCATAATGGAAAATCCATTTGTGAATCTGCAATTATTGTGCAATACATAGATGAAGTTTGGAATAATAAAGCTTCATTCATGCCTTCTAATCCTTATGAGAGAGCTCAAGCTAGATTTTGGGTTGATTATAGTGACAAAAAGGTTTATGACACTTGGAAGAAAATGTGGCTTTCAAAAGGAGAAGAATATGAGGAAGGGAAGATGGAATTGATCTCTATCTTTAAGACACTAGAGGGTGTATTGAGTGACAAGCTTTTTTATGGTGGTGACACATTTGGCTGGTTTTATGCTTTTGAGACATATGGCAACTTCAAAATGGAAACGGAGTGTCCTAAACTAATAGCTTGGACCAAGAGATGCATGGAGAGAGATAGTGTGTCAAAATCTCTTCCTGATCAGAAGAAAGTATTAGATTTAGTGATTTCTACAATCATGGCAAACGAGGTGGTTTTGTTGGATACATGGCCTAGCATGTTTGGTATGAGGGTTAGGATTGCATTAGCTGAAAAGAGTGTCAAATATGAGTACAAAGAAGAAGATCTTAGAAATAAGAGTCCTTTACTTCTTCAGATGAATCCAATTCATAAGAAAATTCCAGTTCTTATTCATAATGGAAAACCTATTTGTGAATCTGCAATTATTGTGCAGTATATAGATGAAGTTTGGAATGATAAAGCTTCATTCATGCCTTCTCACCCTTATGAGAGAGCTCAAGCTAGATTTTGGGTTGATTATATTGACAAAAAGGTGTATGATACTTGGAGGAAAATGTGGCTTTCAAAAGGTGAAGAGCATGAGGAAGGGAAGAAAGAATTGATCTCTATCTTTAAGACACTAGAGGAAACTTTAGGTGACAAGCCTTTTTACGGTGGTGCCACTTTTGGCTTTCTTGATATTGGACTGATCCCCTTTTACAGCTGGTTTTATGCTTATGAGACATATGGTAACTTTAAAATGGAAGTGGAGTGTCCTAAGCTGATGGCATGGGCCAAGAGATGTTTGGAGAAAGAGAGTGTTTCAAAAACTCTTCCTGATCAAATGAAGGTGTATGATTTTGTTGTGAGCATGAAGAAAACACTTGGTTTGGATTAG

Protein sequence

>Msa0253370

MANEVILLDTWASMFGMRARIALAEKSVKYEYKEENLRNKSPLLLQMNPIHKKIPVLIHNGKSICESAIIVQYIDEVWNDKASFMPSNPYERAQARFWVDYSDKKPVLTRQLRIFTIMANEVILLDTWASMFGMRARIALAEKSVKYEYKEEDLRNKSPLLLQMNPIHKKIPVLIHNGKSICESAIIVQYIDEVWNNKASFMPSNPYERAQARFWVDYSDKKVYDTWKKMWLSKGEEYEEGKMELISIFKTLEGVLSDKLFYGGDTFGWFYAFETYGNFKMETECPKLIAWTKRCMERDSVSKSLPDQKKVLDLVISTIMANEVVLLDTWPSMFGMRVRIALAEKSVKYEYKEEDLRNKSPLLLQMNPIHKKIPVLIHNGKPICESAIIVQYIDEVWNDKASFMPSHPYERAQARFWVDYIDKKVYDTWRKMWLSKGEEHEEGKKELISIFKTLEETLGDKPFYGGATFGFLDIGLIPFYSWFYAYETYGNFKMEVECPKLMAWAKRCLEKESVSKTLPDQMKVYDFVVSMKKTLGLD*