AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0862640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862640 MtrunA17_Chr5g0444201 99.091 110 1 0 1 110 1 110 3.63e-78 225
Msa0862640 MtrunA17_Chr3g0098971 84.404 109 17 0 2 110 4 112 3.07e-65 192
Msa0862640 MtrunA17_Chr4g0024171 71.560 109 30 1 3 110 5 113 9.29e-56 168
Msa0862640 MtrunA17_Chr2g0319561 72.072 111 29 1 2 110 4 114 6.68e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862640 AT5G54940.1 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0862640 AT5G54940.3 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0862640 AT5G54940.2 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0862640 AT1G54290.1 72.727 110 29 1 2 110 4 113 8.44e-58 174
Msa0862640 AT4G27130.1 73.636 110 28 1 2 110 4 113 1.13e-57 174
Msa0862640 AT5G54760.2 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0862640 AT5G54760.4 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0862640 AT5G54760.3 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0862640 AT5G54760.1 73.636 110 28 1 2 110 4 113 1.94e-57 173

Find 29 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTTCTTGAGATCTTTA+AGG 0.098306 5_4:+62350980 None:intergenic
CAAAAGAGTATGTGCATATT+AGG 0.278072 5_4:-62351077 Msa0862640:CDS
TCAGTCCTTGCACTGTTGTT+AGG 0.278405 5_4:+62351028 None:intergenic
TAGAAGAGAGTTGAAGAGAT+TGG 0.333969 5_4:+62352194 None:intergenic
AAGGACAACATCAAGATTCA+TGG 0.385779 5_4:-62350844 Msa0862640:CDS
AGAAGAGAGTTGAAGAGATT+GGG 0.422313 5_4:+62352195 None:intergenic
TCATGCTGGTCTTGCTAGAA+AGG 0.426864 5_4:-62350863 Msa0862640:CDS
AGTTGAAGAGATTGGGAATG+TGG 0.436578 5_4:+62352202 None:intergenic
TATCTCTATCTGCAGACTAC+AGG 0.445137 5_4:-62352153 Msa0862640:intron
TGCGTTGATCACCTTGGAGT+TGG 0.445951 5_4:+62350908 None:intergenic
GATTCTTTAGCCTCAGCAAA+TGG 0.452308 5_4:+62351114 None:intergenic
TCTCACTTCCTCATTCATGC+TGG 0.461083 5_4:-62350877 Msa0862640:CDS
GTTGTGAATTGCAAGGATCT+TGG 0.473894 5_4:-62350940 Msa0862640:CDS
GCAAATGGATCAAATGTGCT+TGG 0.480564 5_4:+62351129 None:intergenic
AGAGATTGGGAATGTGGAGG+TGG 0.498637 5_4:+62352208 None:intergenic
CATTCTTGCGTTGATCACCT+TGG 0.510216 5_4:+62350902 None:intergenic
CAAATGGATCAAATGTGCTT+GGG 0.511747 5_4:+62351130 None:intergenic
CACATTTGATCCATTTGCTG+AGG 0.553489 5_4:-62351124 Msa0862640:CDS
AACAGTGCAAGGACTGAAGA+AGG 0.557278 5_4:-62351022 Msa0862640:CDS
ATTAGGATACAACAAAGGAA+TGG 0.567289 5_4:-62351060 Msa0862640:CDS
GAGATTGGGAATGTGGAGGT+GGG 0.587196 5_4:+62352209 None:intergenic
TAGCAAGACCAGCATGAATG+AGG 0.588886 5_4:+62350869 None:intergenic
AATCACAAAGAAAGTGTACA+TGG 0.596673 5_4:-62351166 Msa0862640:exon
GGCAAGATTATCCAACTCCA+AGG 0.618688 5_4:-62350919 Msa0862640:CDS
AAGAGCCTAACAACAGTGCA+AGG 0.631107 5_4:-62351033 Msa0862640:CDS
GCTAAAGAATCAGATGCACC+AGG 0.655042 5_4:-62351102 Msa0862640:CDS
TGGAAATGTTGTGAATTGCA+AGG 0.663364 5_4:-62350947 Msa0862640:CDS
TGAAGAGATTGGGAATGTGG+AGG 0.681590 5_4:+62352205 None:intergenic
TGCATATTAGGATACAACAA+AGG 0.686189 5_4:-62351065 Msa0862640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTATTTTAAAACTAATT+TGG + chr5_4:62351615-62351634 None:intergenic 10.0%
!!! ATTAGTTTTAAAATAAAACT+AGG - chr5_4:62351616-62351635 Msa0862640:intron 10.0%
!! TGATATATATAATTCAAAAT+TGG + chr5_4:62351705-62351724 None:intergenic 10.0%
!!! GTTTTATTTTAAAACTAATT+TGG + chr5_4:62351615-62351634 None:intergenic 10.0%
!!! ATTAGTTTTAAAATAAAACT+AGG - chr5_4:62351616-62351635 Msa0862640:intron 10.0%
!! TGATATATATAATTCAAAAT+TGG + chr5_4:62351705-62351724 None:intergenic 10.0%
!! ATATATATCATATGAATGAA+TGG - chr5_4:62351715-62351734 Msa0862640:intron 15.0%
!!! AAACATAATCTTTAAAACTA+AGG - chr5_4:62351756-62351775 Msa0862640:intron 15.0%
!!! TAATCTGATTTTAATATACA+TGG - chr5_4:62351797-62351816 Msa0862640:intron 15.0%
!! ATATATATCATATGAATGAA+TGG - chr5_4:62351715-62351734 Msa0862640:intron 15.0%
!!! AAACATAATCTTTAAAACTA+AGG - chr5_4:62351756-62351775 Msa0862640:intron 15.0%
!!! TAATCTGATTTTAATATACA+TGG - chr5_4:62351797-62351816 Msa0862640:intron 15.0%
!!! AATATACTCCAAGTTTTATA+TGG - chr5_4:62351088-62351107 Msa0862640:CDS 20.0%
!! TATATTCACCATATAAAACT+TGG + chr5_4:62351099-62351118 None:intergenic 20.0%
!! AGAAAAAAAGTTATGAAGAA+GGG + chr5_4:62351277-62351296 None:intergenic 20.0%
!! AAGAAAAAAAGTTATGAAGA+AGG + chr5_4:62351278-62351297 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_4:62351507-62351526 None:intergenic 20.0%
!!! AATATACTCCAAGTTTTATA+TGG - chr5_4:62351088-62351107 Msa0862640:CDS 20.0%
!! TATATTCACCATATAAAACT+TGG + chr5_4:62351099-62351118 None:intergenic 20.0%
!! AGAAAAAAAGTTATGAAGAA+GGG + chr5_4:62351277-62351296 None:intergenic 20.0%
!! AAGAAAAAAAGTTATGAAGA+AGG + chr5_4:62351278-62351297 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_4:62351507-62351526 None:intergenic 20.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_4:62350997-62351016 Msa0862640:CDS 25.0%
! ATATTTACAATTGTTCCACA+TGG + chr5_4:62351223-62351242 None:intergenic 25.0%
! TAAAACTAGGAATATATGAG+TGG - chr5_4:62351629-62351648 Msa0862640:intron 25.0%
! TATTCACTAATTTCAAACCT+CGG + chr5_4:62351674-62351693 None:intergenic 25.0%
! ATATCATATGAATGAATGGT+TGG - chr5_4:62351719-62351738 Msa0862640:intron 25.0%
! TCAGATTAAAACCAAATCAA+AGG + chr5_4:62351785-62351804 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_4:62351842-62351861 None:intergenic 25.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_4:62350997-62351016 Msa0862640:CDS 25.0%
! ATATTTACAATTGTTCCACA+TGG + chr5_4:62351223-62351242 None:intergenic 25.0%
! TAAAACTAGGAATATATGAG+TGG - chr5_4:62351629-62351648 Msa0862640:intron 25.0%
! TATTCACTAATTTCAAACCT+CGG + chr5_4:62351674-62351693 None:intergenic 25.0%
! ATATCATATGAATGAATGGT+TGG - chr5_4:62351719-62351738 Msa0862640:intron 25.0%
! TCAGATTAAAACCAAATCAA+AGG + chr5_4:62351785-62351804 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_4:62351842-62351861 None:intergenic 25.0%
TAGAATCACCATATCAAACT+TGG + chr5_4:62351008-62351027 None:intergenic 30.0%
AATTCAAACATAGCTCCTTA+AGG + chr5_4:62351200-62351219 None:intergenic 30.0%
TGTGCAAGTTAGAAGATAAA+TGG + chr5_4:62351459-62351478 None:intergenic 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_4:62351880-62351899 Msa0862640:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_4:62351969-62351988 Msa0862640:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_4:62351981-62352000 Msa0862640:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_4:62351986-62352005 Msa0862640:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_4:62352069-62352088 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_4:62352079-62352098 Msa0862640:intron 30.0%
TAGAATCACCATATCAAACT+TGG + chr5_4:62351008-62351027 None:intergenic 30.0%
AATTCAAACATAGCTCCTTA+AGG + chr5_4:62351200-62351219 None:intergenic 30.0%
TGTGCAAGTTAGAAGATAAA+TGG + chr5_4:62351459-62351478 None:intergenic 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_4:62351880-62351899 Msa0862640:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_4:62351969-62351988 Msa0862640:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_4:62351981-62352000 Msa0862640:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_4:62351986-62352005 Msa0862640:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_4:62352069-62352088 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_4:62352079-62352098 Msa0862640:intron 30.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_4:62350854-62350873 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_4:62350855-62350874 None:intergenic 35.0%
TCTTTCCAAGTTTCGACAAT+AGG - chr5_4:62351150-62351169 Msa0862640:CDS 35.0%
TTCAACCTATTGTCGAAACT+TGG + chr5_4:62351158-62351177 None:intergenic 35.0%
AGTTTGAGTCTGTAACCTTA+AGG - chr5_4:62351182-62351201 Msa0862640:intron 35.0%
AGCTATGTTTGAATTCCATG+TGG - chr5_4:62351205-62351224 Msa0862640:intron 35.0%
AACTAAGGAGACCTTTGATT+TGG - chr5_4:62351771-62351790 Msa0862640:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_4:62351919-62351938 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_4:62352099-62352118 Msa0862640:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_4:62352202-62352221 Msa0862640:five_prime_UTR 35.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_4:62350854-62350873 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_4:62350855-62350874 None:intergenic 35.0%
TCTTTCCAAGTTTCGACAAT+AGG - chr5_4:62351150-62351169 Msa0862640:CDS 35.0%
TTCAACCTATTGTCGAAACT+TGG + chr5_4:62351158-62351177 None:intergenic 35.0%
AGTTTGAGTCTGTAACCTTA+AGG - chr5_4:62351182-62351201 Msa0862640:intron 35.0%
AGCTATGTTTGAATTCCATG+TGG - chr5_4:62351205-62351224 Msa0862640:intron 35.0%
AACTAAGGAGACCTTTGATT+TGG - chr5_4:62351771-62351790 Msa0862640:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_4:62351919-62351938 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_4:62352099-62352118 Msa0862640:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_4:62352202-62352221 Msa0862640:five_prime_UTR 35.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_4:62350847-62350866 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_4:62350893-62350912 Msa0862640:CDS 40.0%
TCTCTTGATTGCAAGGATCT+AGG + chr5_4:62351523-62351542 None:intergenic 40.0%
GTGTACTTCTCTTGATTGCA+AGG + chr5_4:62351530-62351549 None:intergenic 40.0%
CGATCTTCATTTAACAACCG+AGG - chr5_4:62351654-62351673 Msa0862640:intron 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_4:62351826-62351845 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_4:62351827-62351846 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_4:62351828-62351847 Msa0862640:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_4:62351920-62351939 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_4:62351922-62351941 Msa0862640:intron 40.0%
GATTCTTTAGCCTCAGCAAA+TGG + chr5_4:62351935-62351954 None:intergenic 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_4:62352106-62352125 Msa0862640:intron 40.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_4:62350847-62350866 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_4:62350893-62350912 Msa0862640:CDS 40.0%
TCTCTTGATTGCAAGGATCT+AGG + chr5_4:62351523-62351542 None:intergenic 40.0%
GTGTACTTCTCTTGATTGCA+AGG + chr5_4:62351530-62351549 None:intergenic 40.0%
CGATCTTCATTTAACAACCG+AGG - chr5_4:62351654-62351673 Msa0862640:intron 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_4:62351826-62351845 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_4:62351827-62351846 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_4:62351828-62351847 Msa0862640:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_4:62351920-62351939 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_4:62351922-62351941 Msa0862640:intron 40.0%
GATTCTTTAGCCTCAGCAAA+TGG + chr5_4:62351935-62351954 None:intergenic 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_4:62352106-62352125 Msa0862640:intron 40.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_4:62350844-62350863 None:intergenic 45.0%
TTAAGGTGCATGCATGCATG+GGG + chr5_4:62351825-62351844 None:intergenic 45.0%
GCTAAAGAATCAGATGCACC+AGG - chr5_4:62351944-62351963 Msa0862640:intron 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_4:62351965-62351984 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_4:62352013-62352032 Msa0862640:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_4:62352021-62352040 None:intergenic 45.0%
AACAGTGCAAGGACTGAAGA+AGG - chr5_4:62352024-62352043 Msa0862640:intron 45.0%
GGCAAGATTATCCAACTCCA+AGG - chr5_4:62352127-62352146 Msa0862640:intron 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_4:62352147-62352166 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_4:62352169-62352188 Msa0862640:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_4:62352180-62352199 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_4:62352183-62352202 Msa0862640:five_prime_UTR 45.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_4:62350844-62350863 None:intergenic 45.0%
TTAAGGTGCATGCATGCATG+GGG + chr5_4:62351825-62351844 None:intergenic 45.0%
GCTAAAGAATCAGATGCACC+AGG - chr5_4:62351944-62351963 Msa0862640:intron 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_4:62351965-62351984 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_4:62352013-62352032 Msa0862640:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_4:62352021-62352040 None:intergenic 45.0%
AACAGTGCAAGGACTGAAGA+AGG - chr5_4:62352024-62352043 Msa0862640:intron 45.0%
GGCAAGATTATCCAACTCCA+AGG - chr5_4:62352127-62352146 Msa0862640:intron 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_4:62352147-62352166 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_4:62352169-62352188 Msa0862640:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_4:62352180-62352199 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_4:62352183-62352202 Msa0862640:five_prime_UTR 45.0%
!!! ATTTTAACAATTATATTTTT+TGG - chr5_4:62351386-62351405 Msa0862640:intron 5.0%
!!! ATTTTAACAATTATATTTTT+TGG - chr5_4:62351386-62351405 Msa0862640:intron 5.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_4:62350840-62350859 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_4:62350841-62350860 None:intergenic 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_4:62352141-62352160 None:intergenic 50.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_4:62350840-62350859 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_4:62350841-62350860 None:intergenic 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_4:62352141-62352160 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 62350837 62352231 62350837 ID=Msa0862640;Name=Msa0862640
chr5_4 mRNA 62350837 62352231 62350837 ID=Msa0862640-mRNA-1;Parent=Msa0862640;Name=Msa0862640-mRNA-1;_AED=0.15;_eAED=0.21;_QI=101|0|0.5|1|0|0|2|0|110
chr5_4 exon 62350837 62351192 62350837 ID=Msa0862640-mRNA-1:exon:19464;Parent=Msa0862640-mRNA-1
chr5_4 exon 62352154 62352231 62352154 ID=Msa0862640-mRNA-1:exon:19463;Parent=Msa0862640-mRNA-1
chr5_4 five_prime_UTR 62352154 62352231 62352154 ID=Msa0862640-mRNA-1:five_prime_utr;Parent=Msa0862640-mRNA-1
chr5_4 five_prime_UTR 62351170 62351192 62351170 ID=Msa0862640-mRNA-1:five_prime_utr;Parent=Msa0862640-mRNA-1
chr5_4 CDS 62350837 62351169 62350837 ID=Msa0862640-mRNA-1:cds;Parent=Msa0862640-mRNA-1
Gene Sequence

>Msa0862640

ATGGTTGATATTCAGATCCCAAGCACATTTGATCCATTTGCTGAGGCTAAAGAATCAGATGCACCAGGTGCAAAAGAGTATGTGCATATTAGGATACAACAAAGGAATGGAAAAAAGAGCCTAACAACAGTGCAAGGACTGAAGAAGGAGTTCAGCTACGAGAAGATCCTTAAAGATCTCAAGAAAGAGTTTTGTTGCAATGGAAATGTTGTGAATTGCAAGGATCTTGGCAAGATTATCCAACTCCAAGGTGATCAACGCAAGAATGTTTCTCACTTCCTCATTCATGCTGGTCTTGCTAGAAAGGACAACATCAAGATTCATGGTTTTTAA

Protein sequence

>Msa0862640

MVDIQIPSTFDPFAEAKESDAPGAKEYVHIRIQQRNGKKSLTTVQGLKKEFSYEKILKDLKKEFCCNGNVVNCKDLGKIIQLQGDQRKNVSHFLIHAGLARKDNIKIHGF*