AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1244830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244830 MtrunA17_Chr8g0360121 100.000 63 0 0 1 63 1 63 5.96e-41 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1244830 AT3G15353.1 57.576 66 23 2 1 61 1 66 1.04e-17 69.7
Msa1244830 AT3G15353.2 56.923 65 23 2 1 61 1 64 5.29e-17 67.8

Find 24 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGCATATATTCTTTAA+TGG 0.154703 8_2:-34138590 None:intergenic
AATTCCGATTCAACCAAAAT+AGG 0.225109 8_2:-34138800 None:intergenic
AAATGTAATTGCCATGAATT+TGG 0.244815 8_2:+34138608 Msa1244830:three_prime_UTR
ATTCCGATTCAACCAAAATA+GGG 0.298298 8_2:-34138799 None:intergenic
ATGAGCTTGACATGTTTGTT+TGG 0.442431 8_2:-34137885 None:intergenic
TAAGCCGTAAACAAAAGTTA+TGG 0.503942 8_2:-34138760 None:intergenic
TTGCAATCATGCTCAACAGC+TGG 0.507300 8_2:-34138528 None:intergenic
TATCACAATTTACCATGTTG+TGG 0.518254 8_2:+34138714 Msa1244830:three_prime_UTR
CTTCGTCGAGACTGTTGTCA+TGG 0.555720 8_2:+34138500 Msa1244830:CDS
ATATATTCTTTAATGGCCGC+AGG 0.559172 8_2:-34138583 None:intergenic
AGAGGAGCACTTACCCGCAC+TGG 0.574771 8_2:-34137936 None:intergenic
GTTGAGCATGATTGCAAGTG+TGG 0.581040 8_2:+34138534 Msa1244830:CDS
TTCAGGAAGGGAAACAACTA+CGG 0.588697 8_2:+34138336 Msa1244830:intron
GCGCTGACAAGAGCCAGTGC+GGG 0.597634 8_2:+34137923 Msa1244830:CDS
TTCCGATTCAACCAAAATAG+GGG 0.599277 8_2:-34138798 None:intergenic
ACAAACATGTCAAGCTCATG+CGG 0.602184 8_2:+34137889 Msa1244830:exon
TCCGATTCAACCAAAATAGG+GGG 0.602779 8_2:-34138797 None:intergenic
TGCGCTGACAAGAGCCAGTG+CGG 0.611618 8_2:+34137922 Msa1244830:CDS
CGATTCAACCAAAATAGGGG+GGG 0.614421 8_2:-34138795 None:intergenic
AACAACAGAATGCCACAACA+TGG 0.646930 8_2:-34138726 None:intergenic
CCGATTCAACCAAAATAGGG+GGG 0.647274 8_2:-34138796 None:intergenic
ACTTGCACTAACTGCACCTG+CGG 0.656458 8_2:+34138567 Msa1244830:CDS
CCTCTTCTGAGTCTCGACAA+TGG 0.679042 8_2:-34138364 None:intergenic
CCATTGTCGAGACTCAGAAG+AGG 0.690774 8_2:+34138364 Msa1244830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGGTTAATGTGTATTTTAT+TGG + chr8_2:34137971-34137990 Msa1244830:intron 20.0%
!! ATAAACACTTATGATATAAG+TGG + chr8_2:34138084-34138103 Msa1244830:intron 20.0%
!! GAGTTTATAAAAATAGCTTA+TGG - chr8_2:34138205-34138224 None:intergenic 20.0%
!! CATTTGCATATATTCTTTAA+TGG - chr8_2:34138593-34138612 None:intergenic 20.0%
!!! TTAACCATAACTTTTGTTTA+CGG + chr8_2:34138756-34138775 Msa1244830:three_prime_UTR 20.0%
!!! AAGGTTAATGTGTATTTTAT+TGG + chr8_2:34137971-34137990 Msa1244830:intron 20.0%
!! ATAAACACTTATGATATAAG+TGG + chr8_2:34138084-34138103 Msa1244830:intron 20.0%
!! GAGTTTATAAAAATAGCTTA+TGG - chr8_2:34138205-34138224 None:intergenic 20.0%
!! CATTTGCATATATTCTTTAA+TGG - chr8_2:34138593-34138612 None:intergenic 20.0%
!!! TTAACCATAACTTTTGTTTA+CGG + chr8_2:34138756-34138775 Msa1244830:three_prime_UTR 20.0%
! TTTAGAAAAACGGCTTAATT+AGG + chr8_2:34138051-34138070 Msa1244830:intron 25.0%
! AAATGTAATTGCCATGAATT+TGG + chr8_2:34138608-34138627 Msa1244830:three_prime_UTR 25.0%
!! ATTTTGTATTGCCAAATTCA+TGG - chr8_2:34138622-34138641 None:intergenic 25.0%
! TTTAGAAAAACGGCTTAATT+AGG + chr8_2:34138051-34138070 Msa1244830:intron 25.0%
! AAATGTAATTGCCATGAATT+TGG + chr8_2:34138608-34138627 Msa1244830:three_prime_UTR 25.0%
!! ATTTTGTATTGCCAAATTCA+TGG - chr8_2:34138622-34138641 None:intergenic 25.0%
!!! TTAAGCCGTTTTTCTAAACA+AGG - chr8_2:34138049-34138068 None:intergenic 30.0%
! TTGTTTTCATAAGCTGAAGT+CGG + chr8_2:34138167-34138186 Msa1244830:intron 30.0%
TACCATAGCATCATATATGA+GGG - chr8_2:34138316-34138335 None:intergenic 30.0%
ATACCATAGCATCATATATG+AGG - chr8_2:34138317-34138336 None:intergenic 30.0%
TATATGATGCTATGGTATTC+AGG + chr8_2:34138319-34138338 Msa1244830:intron 30.0%
TATCACAATTTACCATGTTG+TGG + chr8_2:34138714-34138733 Msa1244830:three_prime_UTR 30.0%
TAAGCCGTAAACAAAAGTTA+TGG - chr8_2:34138763-34138782 None:intergenic 30.0%
ATTCCGATTCAACCAAAATA+GGG - chr8_2:34138802-34138821 None:intergenic 30.0%
AATTCCGATTCAACCAAAAT+AGG - chr8_2:34138803-34138822 None:intergenic 30.0%
!!! TTAAGCCGTTTTTCTAAACA+AGG - chr8_2:34138049-34138068 None:intergenic 30.0%
! TTGTTTTCATAAGCTGAAGT+CGG + chr8_2:34138167-34138186 Msa1244830:intron 30.0%
TACCATAGCATCATATATGA+GGG - chr8_2:34138316-34138335 None:intergenic 30.0%
ATACCATAGCATCATATATG+AGG - chr8_2:34138317-34138336 None:intergenic 30.0%
TATATGATGCTATGGTATTC+AGG + chr8_2:34138319-34138338 Msa1244830:intron 30.0%
TATCACAATTTACCATGTTG+TGG + chr8_2:34138714-34138733 Msa1244830:three_prime_UTR 30.0%
TAAGCCGTAAACAAAAGTTA+TGG - chr8_2:34138763-34138782 None:intergenic 30.0%
ATTCCGATTCAACCAAAATA+GGG - chr8_2:34138802-34138821 None:intergenic 30.0%
AATTCCGATTCAACCAAAAT+AGG - chr8_2:34138803-34138822 None:intergenic 30.0%
ATGAGCTTGACATGTTTGTT+TGG - chr8_2:34137888-34137907 None:intergenic 35.0%
AACCTTACAGTACAAGATAG+AGG - chr8_2:34137957-34137976 None:intergenic 35.0%
GCGTTCACTCATTTATATAG+AGG + chr8_2:34138023-34138042 Msa1244830:intron 35.0%
!! AGAGGCCTTGTTTAGAAAAA+CGG + chr8_2:34138041-34138060 Msa1244830:intron 35.0%
CAGCTTATGAAAACAACTTC+AGG - chr8_2:34138163-34138182 None:intergenic 35.0%
ACCATAGCATCATATATGAG+GGG - chr8_2:34138315-34138334 None:intergenic 35.0%
TCGAGGAGTTATTAGAAACA+TGG - chr8_2:34138419-34138438 None:intergenic 35.0%
ATATATTCTTTAATGGCCGC+AGG - chr8_2:34138586-34138605 None:intergenic 35.0%
TTCCGATTCAACCAAAATAG+GGG - chr8_2:34138801-34138820 None:intergenic 35.0%
ATGAGCTTGACATGTTTGTT+TGG - chr8_2:34137888-34137907 None:intergenic 35.0%
AACCTTACAGTACAAGATAG+AGG - chr8_2:34137957-34137976 None:intergenic 35.0%
GCGTTCACTCATTTATATAG+AGG + chr8_2:34138023-34138042 Msa1244830:intron 35.0%
!! AGAGGCCTTGTTTAGAAAAA+CGG + chr8_2:34138041-34138060 Msa1244830:intron 35.0%
CAGCTTATGAAAACAACTTC+AGG - chr8_2:34138163-34138182 None:intergenic 35.0%
ACCATAGCATCATATATGAG+GGG - chr8_2:34138315-34138334 None:intergenic 35.0%
TCGAGGAGTTATTAGAAACA+TGG - chr8_2:34138419-34138438 None:intergenic 35.0%
ATATATTCTTTAATGGCCGC+AGG - chr8_2:34138586-34138605 None:intergenic 35.0%
TTCCGATTCAACCAAAATAG+GGG - chr8_2:34138801-34138820 None:intergenic 35.0%
ACAAACATGTCAAGCTCATG+CGG + chr8_2:34137889-34137908 Msa1244830:exon 40.0%
CTCCTCTATCTTGTACTGTA+AGG + chr8_2:34137952-34137971 Msa1244830:intron 40.0%
! TACGGATTTGTTTGTGAGAC+TGG - chr8_2:34138232-34138251 None:intergenic 40.0%
TCTCACAAACAAATCCGTAC+AGG + chr8_2:34138233-34138252 Msa1244830:intron 40.0%
TCCCCTCATATATGATGCTA+TGG + chr8_2:34138311-34138330 Msa1244830:intron 40.0%
TGATGCTATGGTATTCAGGA+AGG + chr8_2:34138323-34138342 Msa1244830:intron 40.0%
GATGCTATGGTATTCAGGAA+GGG + chr8_2:34138324-34138343 Msa1244830:intron 40.0%
TTCAGGAAGGGAAACAACTA+CGG + chr8_2:34138336-34138355 Msa1244830:intron 40.0%
AACAACAGAATGCCACAACA+TGG - chr8_2:34138729-34138748 None:intergenic 40.0%
TCCGATTCAACCAAAATAGG+GGG - chr8_2:34138800-34138819 None:intergenic 40.0%
AATTGAATGTTGCTGCTGTG+CGG - chr8_2:34137835-34137854 None:intergenic 40.0%
ACAAACATGTCAAGCTCATG+CGG + chr8_2:34137889-34137908 Msa1244830:exon 40.0%
CTCCTCTATCTTGTACTGTA+AGG + chr8_2:34137952-34137971 Msa1244830:intron 40.0%
! TACGGATTTGTTTGTGAGAC+TGG - chr8_2:34138232-34138251 None:intergenic 40.0%
TCTCACAAACAAATCCGTAC+AGG + chr8_2:34138233-34138252 Msa1244830:intron 40.0%
TCCCCTCATATATGATGCTA+TGG + chr8_2:34138311-34138330 Msa1244830:intron 40.0%
TGATGCTATGGTATTCAGGA+AGG + chr8_2:34138323-34138342 Msa1244830:intron 40.0%
GATGCTATGGTATTCAGGAA+GGG + chr8_2:34138324-34138343 Msa1244830:intron 40.0%
TTCAGGAAGGGAAACAACTA+CGG + chr8_2:34138336-34138355 Msa1244830:intron 40.0%
AACAACAGAATGCCACAACA+TGG - chr8_2:34138729-34138748 None:intergenic 40.0%
TCCGATTCAACCAAAATAGG+GGG - chr8_2:34138800-34138819 None:intergenic 40.0%
! CATAAGCTGAAGTCGGCTTA+TGG + chr8_2:34138174-34138193 Msa1244830:intron 45.0%
AACAAATCCGTACAGGCTTG+TGG + chr8_2:34138240-34138259 Msa1244830:intron 45.0%
!! CTAGCTTTTGCATTGAGTCG+AGG - chr8_2:34138436-34138455 None:intergenic 45.0%
TTGCAATCATGCTCAACAGC+TGG - chr8_2:34138531-34138550 None:intergenic 45.0%
GTTGAGCATGATTGCAAGTG+TGG + chr8_2:34138534-34138553 Msa1244830:CDS 45.0%
! CAATTTTGCCCCCCCTATTT+TGG + chr8_2:34138787-34138806 Msa1244830:three_prime_UTR 45.0%
CGATTCAACCAAAATAGGGG+GGG - chr8_2:34138798-34138817 None:intergenic 45.0%
CCGATTCAACCAAAATAGGG+GGG - chr8_2:34138799-34138818 None:intergenic 45.0%
!!! CCCCCCTATTTTGGTTGAAT+CGG + chr8_2:34138796-34138815 Msa1244830:three_prime_UTR 45.0%
ATTGAATGTTGCTGCTGTGC+GGG - chr8_2:34137834-34137853 None:intergenic 45.0%
! CATAAGCTGAAGTCGGCTTA+TGG + chr8_2:34138174-34138193 Msa1244830:intron 45.0%
AACAAATCCGTACAGGCTTG+TGG + chr8_2:34138240-34138259 Msa1244830:intron 45.0%
!! CTAGCTTTTGCATTGAGTCG+AGG - chr8_2:34138436-34138455 None:intergenic 45.0%
TTGCAATCATGCTCAACAGC+TGG - chr8_2:34138531-34138550 None:intergenic 45.0%
GTTGAGCATGATTGCAAGTG+TGG + chr8_2:34138534-34138553 Msa1244830:CDS 45.0%
! CAATTTTGCCCCCCCTATTT+TGG + chr8_2:34138787-34138806 Msa1244830:three_prime_UTR 45.0%
CGATTCAACCAAAATAGGGG+GGG - chr8_2:34138798-34138817 None:intergenic 45.0%
CCGATTCAACCAAAATAGGG+GGG - chr8_2:34138799-34138818 None:intergenic 45.0%
!!! CCCCCCTATTTTGGTTGAAT+CGG + chr8_2:34138796-34138815 Msa1244830:three_prime_UTR 45.0%
!!! TATTTCTATAAATAAAATAA+AGG + chr8_2:34138119-34138138 Msa1244830:intron 5.0%
!!! TATTTCTATAAATAAAATAA+AGG + chr8_2:34138119-34138138 Msa1244830:intron 5.0%
CTACAAGCCACAAGCCTGTA+CGG - chr8_2:34138250-34138269 None:intergenic 50.0%
CCTCTTCTGAGTCTCGACAA+TGG - chr8_2:34138367-34138386 None:intergenic 50.0%
CCATTGTCGAGACTCAGAAG+AGG + chr8_2:34138364-34138383 Msa1244830:CDS 50.0%
CTTCGTCGAGACTGTTGTCA+TGG + chr8_2:34138500-34138519 Msa1244830:CDS 50.0%
ACTTGCACTAACTGCACCTG+CGG + chr8_2:34138567-34138586 Msa1244830:CDS 50.0%
CTACAAGCCACAAGCCTGTA+CGG - chr8_2:34138250-34138269 None:intergenic 50.0%
CCTCTTCTGAGTCTCGACAA+TGG - chr8_2:34138367-34138386 None:intergenic 50.0%
CCATTGTCGAGACTCAGAAG+AGG + chr8_2:34138364-34138383 Msa1244830:CDS 50.0%
CTTCGTCGAGACTGTTGTCA+TGG + chr8_2:34138500-34138519 Msa1244830:CDS 50.0%
ACTTGCACTAACTGCACCTG+CGG + chr8_2:34138567-34138586 Msa1244830:CDS 50.0%
! TGCGCTGACAAGAGCCAGTG+CGG + chr8_2:34137922-34137941 Msa1244830:CDS 60.0%
!! AGAGGAGCACTTACCCGCAC+TGG - chr8_2:34137939-34137958 None:intergenic 60.0%
! TGCGCTGACAAGAGCCAGTG+CGG + chr8_2:34137922-34137941 Msa1244830:CDS 60.0%
!! AGAGGAGCACTTACCCGCAC+TGG - chr8_2:34137939-34137958 None:intergenic 60.0%
! GCGCTGACAAGAGCCAGTGC+GGG + chr8_2:34137923-34137942 Msa1244830:CDS 65.0%
! GCGCTGACAAGAGCCAGTGC+GGG + chr8_2:34137923-34137942 Msa1244830:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 34137834 34138831 34137834 ID=Msa1244830;Name=Msa1244830
chr8_2 mRNA 34137834 34138831 34137834 ID=Msa1244830-mRNA-1;Parent=Msa1244830;Name=Msa1244830-mRNA-1;_AED=0.09;_eAED=0.09;_QI=61|1|1|1|1|1|3|235|63
chr8_2 exon 34137834 34137944 34137834 ID=Msa1244830-mRNA-1:exon:10887;Parent=Msa1244830-mRNA-1
chr8_2 exon 34138341 34138385 34138341 ID=Msa1244830-mRNA-1:exon:10888;Parent=Msa1244830-mRNA-1
chr8_2 exon 34138500 34138831 34138500 ID=Msa1244830-mRNA-1:exon:10889;Parent=Msa1244830-mRNA-1
chr8_2 five_prime_UTR 34137834 34137894 34137834 ID=Msa1244830-mRNA-1:five_prime_utr;Parent=Msa1244830-mRNA-1
chr8_2 CDS 34137895 34137944 34137895 ID=Msa1244830-mRNA-1:cds;Parent=Msa1244830-mRNA-1
chr8_2 CDS 34138341 34138385 34138341 ID=Msa1244830-mRNA-1:cds;Parent=Msa1244830-mRNA-1
chr8_2 CDS 34138500 34138596 34138500 ID=Msa1244830-mRNA-1:cds;Parent=Msa1244830-mRNA-1
chr8_2 three_prime_UTR 34138597 34138831 34138597 ID=Msa1244830-mRNA-1:three_prime_utr;Parent=Msa1244830-mRNA-1
Gene Sequence

>Msa1244830

ATGTCAAGCTCATGCGGCAACTGCGACTGCGCTGACAAGAGCCAGTGCGGGAAGGGAAACAACTACGGCATGACCATTGTCGAGACTCAGAAGAGCTTCGTCGAGACTGTTGTCATGGATGCTCCAGCTGTTGAGCATGATTGCAAGTGTGGTTCTAACTGCACTTGCACTAACTGCACCTGCGGCCATTAA

Protein sequence

>Msa1244830

MSSSCGNCDCADKSQCGKGNNYGMTIVETQKSFVETVVMDAPAVEHDCKCGSNCTCTNCTCGH*