AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0838490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838490 MtrunA17_Chr5g0406071 91.279 172 14 1 1 172 1 171 3.73e-107 303
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838490 AT2G17710.1 50.360 139 43 3 36 172 38 152 1.71e-38 129

Find 66 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCCATGTGTGTTATT+TGG 0.092661 5_4:-12876640 Msa0838490:three_prime_UTR
GTCACACTCTCTTCTGTTCT+TGG 0.123272 5_4:+12878351 None:intergenic
TATGTCAACTGGTCAATTTC+TGG 0.137128 5_4:-12876704 Msa0838490:three_prime_UTR
CAAATTGATAGTCTCTTTAT+TGG 0.141487 5_4:+12877853 None:intergenic
ATCACCAGTAATAGAATTTC+TGG 0.152523 5_4:-12876523 Msa0838490:three_prime_UTR
TTTGAGATGATTCGGAATTT+TGG 0.208841 5_4:+12878375 None:intergenic
GTTCTCCCCACGCAAGTAAA+AGG 0.227857 5_4:-12878660 Msa0838490:five_prime_UTR
AAATCCCATGTGTGTTATTT+GGG 0.285292 5_4:-12876639 Msa0838490:three_prime_UTR
TAAGTAGAGGTCCGTTTCTT+AGG 0.285599 5_4:+12876815 None:intergenic
AGCGCGAAATTGCCAAGTTT+TGG 0.285984 5_4:-12878326 Msa0838490:CDS
CAATAAAGAGACTATCAATT+TGG 0.299593 5_4:-12877852 Msa0838490:CDS
GCTTTGTATCCTACTGCTAT+TGG 0.336710 5_4:-12876761 Msa0838490:CDS
ATGTCCAGAAATTCTATTAC+TGG 0.344567 5_4:+12876519 None:intergenic
CTAAAATAGATTTGAGATTA+CGG 0.369925 5_4:+12878526 None:intergenic
ATAATATCGAGTGCCTCTTA+TGG 0.373495 5_4:+12876726 None:intergenic
ATTGTTACATTTGTTACAAC+TGG 0.377760 5_4:-12876610 Msa0838490:three_prime_UTR
GAGACTATCAATTTGGTTGT+AGG 0.381164 5_4:-12877845 Msa0838490:CDS
AGCGGAGGAGGAGGAGGTGG+TGG 0.385556 5_4:+12878399 None:intergenic
TTTGGAAGCGGAGGAGGAGG+AGG 0.392926 5_4:+12878393 None:intergenic
GGAAGCGGAGGAGGAGGAGG+TGG 0.398218 5_4:+12878396 None:intergenic
TATTCTTTCTTACAAGCATA+AGG 0.399281 5_4:-12876783 Msa0838490:CDS
AGACTATCAATTTGGTTGTA+GGG 0.440855 5_4:-12877844 Msa0838490:CDS
TCATCTCCTTGCTGCTATCA+AGG 0.456611 5_4:-12878277 Msa0838490:CDS
TTCTCCCCACGCAAGTAAAA+GGG 0.456820 5_4:-12878659 Msa0838490:five_prime_UTR
ACTCGATATTATATGTCAAC+TGG 0.464577 5_4:-12876715 Msa0838490:three_prime_UTR
AGGAACAAGCAACCGTTTCA+AGG 0.465570 5_4:-12878431 Msa0838490:CDS
TTAAGCTCAGTTCAAACCTC+AGG 0.471088 5_4:+12877900 None:intergenic
CCAACCAGCCTTAGCTTCCA+TGG 0.474612 5_4:-12876843 Msa0838490:CDS
TGTTGAAATGAAGCAAAGAA+AGG 0.479451 5_4:-12878451 Msa0838490:exon
TGAACTGAGCTTAAAGAATG+AGG 0.483526 5_4:-12877891 Msa0838490:CDS
AGTGTGGAAGAAAGGTAGGT+TGG 0.504682 5_4:-12878567 Msa0838490:five_prime_UTR
GACTATCAATTTGGTTGTAG+GGG 0.511564 5_4:-12877843 Msa0838490:CDS
AGGAGGATCCATGGAAGCTA+AGG 0.520566 5_4:+12876835 None:intergenic
ACTGGTGTAGTGTGGAAGAA+AGG 0.529142 5_4:-12878575 Msa0838490:five_prime_UTR
TTACCGTGAGGTTGCGCGCA+CGG 0.533446 5_4:+12878241 None:intergenic
CCGTTTCTTAGGAGGATCCA+TGG 0.535644 5_4:+12876826 None:intergenic
TGGTGTTGCTGCCCTTGAAA+CGG 0.539688 5_4:+12878419 None:intergenic
GTAGAGGTCCGTTTCTTAGG+AGG 0.541548 5_4:+12876818 None:intergenic
TGCGCGTGGGAATAAAAGAT+TGG 0.546641 5_4:-12877781 Msa0838490:intron
CCATGGATCCTCCTAAGAAA+CGG 0.550809 5_4:-12876826 Msa0838490:CDS
CTTATCCCAAATAACACACA+TGG 0.550961 5_4:+12876634 None:intergenic
TGCTTGTAAGAAAGAATATG+TGG 0.555711 5_4:+12876788 None:intergenic
GAACTGAGCTTAAAGAATGA+GGG 0.561286 5_4:-12877890 Msa0838490:CDS
GCAAAAGCGAATTGTGGAAG+AGG 0.567524 5_4:-12878301 Msa0838490:CDS
TGCAAATGGAATTTCTTAGT+CGG 0.570973 5_4:+12878613 None:intergenic
CCATGGAAGCTAAGGCTGGT+TGG 0.571317 5_4:+12876843 None:intergenic
GTCTATCCACTGGTGTAGTG+TGG 0.573779 5_4:-12878583 Msa0838490:five_prime_UTR
GGAACAAGCAACCGTTTCAA+GGG 0.579284 5_4:-12878430 Msa0838490:CDS
GTGTAGTGTGGAAGAAAGGT+AGG 0.583297 5_4:-12878571 Msa0838490:five_prime_UTR
GGATCCATGGAAGCTAAGGC+TGG 0.586903 5_4:+12876839 None:intergenic
ACTCCGTGCGCGCAACCTCA+CGG 0.592771 5_4:-12878244 Msa0838490:intron
CAAAAGACACCAATAGCAGT+AGG 0.596240 5_4:+12876752 None:intergenic
TTGTGGCAATAAAGTTGATG+AGG 0.597067 5_4:-12877813 Msa0838490:CDS
GTTGTAGGGGCAAATTATTG+TGG 0.611103 5_4:-12877830 Msa0838490:CDS
TTTCTTCCACACTACACCAG+TGG 0.623453 5_4:+12878577 None:intergenic
CTGCAGCCTTGATAGCAGCA+AGG 0.633031 5_4:+12878271 None:intergenic
GAATGAGGGTGATGAACATG+AGG 0.634033 5_4:-12877876 Msa0838490:CDS
TATAGGAGCAAGAGTACCTG+AGG 0.641291 5_4:-12877916 Msa0838490:intron
AATATGTGGAACATAAGTAG+AGG 0.646153 5_4:+12876802 None:intergenic
TTGGAGGCAAAAGCGAATTG+TGG 0.647122 5_4:-12878307 Msa0838490:CDS
TGAGGACAAAGAAGTGCGCG+TGG 0.647667 5_4:-12877795 Msa0838490:CDS
AACAAATGAAACATTAACAA+GGG 0.647858 5_4:+12876667 None:intergenic
TAACAAATGAAACATTAACA+AGG 0.656419 5_4:+12876666 None:intergenic
TCTCCCCACGCAAGTAAAAG+GGG 0.658819 5_4:-12878658 Msa0838490:five_prime_UTR
TTATCCCAAATAACACACAT+GGG 0.689145 5_4:+12876635 None:intergenic
GAGGACAAAGAAGTGCGCGT+GGG 0.694290 5_4:-12877794 Msa0838490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATCATTTTATTTTTTTATG+CGG + chr5_4:12877077-12877096 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_4:12877900-12877919 Msa0838490:CDS 10.0%
!!! TATTTTTTTTTTATGAGAAT+TGG + chr5_4:12878011-12878030 None:intergenic 10.0%
!! TCATAAAAAAAAAATACTAA+TGG - chr5_4:12878015-12878034 Msa0838490:intron 10.0%
!!! AATCATTTTATTTTTTTATG+CGG + chr5_4:12877077-12877096 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_4:12877900-12877919 Msa0838490:CDS 10.0%
!!! TATTTTTTTTTTATGAGAAT+TGG + chr5_4:12878011-12878030 None:intergenic 10.0%
!! TCATAAAAAAAAAATACTAA+TGG - chr5_4:12878015-12878034 Msa0838490:intron 10.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_4:12877016-12877035 Msa0838490:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_4:12877139-12877158 None:intergenic 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_4:12877249-12877268 Msa0838490:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_4:12877489-12877508 Msa0838490:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_4:12877627-12877646 Msa0838490:intron 15.0%
!! AATTGAAATTTAGAAGATTT+AGG - chr5_4:12877982-12878001 Msa0838490:intron 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_4:12878074-12878093 Msa0838490:intron 15.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_4:12877016-12877035 Msa0838490:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_4:12877139-12877158 None:intergenic 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_4:12877249-12877268 Msa0838490:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_4:12877489-12877508 Msa0838490:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_4:12877627-12877646 Msa0838490:intron 15.0%
!! AATTGAAATTTAGAAGATTT+AGG - chr5_4:12877982-12878001 Msa0838490:intron 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_4:12878074-12878093 Msa0838490:intron 15.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_4:12876659-12876678 None:intergenic 20.0%
!! ATTAACATATATTCTTCATG+TGG - chr5_4:12877164-12877183 Msa0838490:intron 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_4:12877574-12877593 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_4:12878224-12878243 Msa0838490:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_4:12878518-12878537 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_4:12878519-12878538 None:intergenic 20.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_4:12876659-12876678 None:intergenic 20.0%
!! ATTAACATATATTCTTCATG+TGG - chr5_4:12877164-12877183 Msa0838490:intron 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_4:12877574-12877593 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_4:12878224-12878243 Msa0838490:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_4:12878518-12878537 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_4:12878519-12878538 None:intergenic 20.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_4:12877135-12877154 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_4:12877332-12877351 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_4:12877330-12877349 Msa0838490:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_4:12877413-12877432 Msa0838490:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_4:12877446-12877465 Msa0838490:intron 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_4:12877778-12877797 None:intergenic 25.0%
! ACAAGATAACATCATAGAAA+AGG + chr5_4:12878188-12878207 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_4:12878238-12878257 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_4:12878399-12878418 Msa0838490:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_4:12878572-12878591 Msa0838490:five_prime_UTR 25.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_4:12877135-12877154 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_4:12877332-12877351 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_4:12877330-12877349 Msa0838490:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_4:12877413-12877432 Msa0838490:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_4:12877446-12877465 Msa0838490:intron 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_4:12877778-12877797 None:intergenic 25.0%
! ACAAGATAACATCATAGAAA+AGG + chr5_4:12878188-12878207 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_4:12878238-12878257 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_4:12878399-12878418 Msa0838490:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_4:12878572-12878591 Msa0838490:five_prime_UTR 25.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_4:12876572-12876591 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_4:12876586-12876605 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_4:12876731-12876750 Msa0838490:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_4:12876810-12876829 None:intergenic 30.0%
!!! TTCTTGGTTTTGAGATGATT+CGG + chr5_4:12876818-12876837 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_4:12877338-12877357 Msa0838490:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_4:12877452-12877471 Msa0838490:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_4:12877636-12877655 Msa0838490:intron 30.0%
! TTGATTTAGTTCCAATAGTC+CGG - chr5_4:12877686-12877705 Msa0838490:intron 30.0%
GATTGAATCAGGATGAATTA+TGG + chr5_4:12877744-12877763 None:intergenic 30.0%
ATCAAGCTATAGATTGAATC+AGG + chr5_4:12877755-12877774 None:intergenic 30.0%
!!! CTGTCTAGTTTCATTTTTTG+TGG + chr5_4:12877926-12877945 None:intergenic 30.0%
ATTATCACTGAGATTAAAGC+AGG + chr5_4:12877959-12877978 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_4:12878041-12878060 Msa0838490:intron 30.0%
AACATCATAGAAAAGGTTGT+GGG + chr5_4:12878181-12878200 None:intergenic 30.0%
TAACATCATAGAAAAGGTTG+TGG + chr5_4:12878182-12878201 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_4:12878287-12878306 Msa0838490:CDS 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_4:12878383-12878402 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_4:12878397-12878416 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_4:12878467-12878486 Msa0838490:five_prime_UTR 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_4:12878542-12878561 Msa0838490:five_prime_UTR 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_4:12878543-12878562 Msa0838490:five_prime_UTR 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_4:12878550-12878569 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_4:12878629-12878648 Msa0838490:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_4:12878659-12878678 Msa0838490:five_prime_UTR 30.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_4:12876572-12876591 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_4:12876586-12876605 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_4:12876731-12876750 Msa0838490:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_4:12876810-12876829 None:intergenic 30.0%
!!! TTCTTGGTTTTGAGATGATT+CGG + chr5_4:12876818-12876837 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_4:12877338-12877357 Msa0838490:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_4:12877452-12877471 Msa0838490:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_4:12877636-12877655 Msa0838490:intron 30.0%
! TTGATTTAGTTCCAATAGTC+CGG - chr5_4:12877686-12877705 Msa0838490:intron 30.0%
GATTGAATCAGGATGAATTA+TGG + chr5_4:12877744-12877763 None:intergenic 30.0%
ATCAAGCTATAGATTGAATC+AGG + chr5_4:12877755-12877774 None:intergenic 30.0%
!!! CTGTCTAGTTTCATTTTTTG+TGG + chr5_4:12877926-12877945 None:intergenic 30.0%
ATTATCACTGAGATTAAAGC+AGG + chr5_4:12877959-12877978 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_4:12878041-12878060 Msa0838490:intron 30.0%
AACATCATAGAAAAGGTTGT+GGG + chr5_4:12878181-12878200 None:intergenic 30.0%
TAACATCATAGAAAAGGTTG+TGG + chr5_4:12878182-12878201 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_4:12878287-12878306 Msa0838490:CDS 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_4:12878383-12878402 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_4:12878397-12878416 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_4:12878467-12878486 Msa0838490:five_prime_UTR 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_4:12878542-12878561 Msa0838490:five_prime_UTR 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_4:12878543-12878562 Msa0838490:five_prime_UTR 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_4:12878550-12878569 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_4:12878629-12878648 Msa0838490:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_4:12878659-12878678 Msa0838490:five_prime_UTR 30.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_4:12876804-12876823 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_4:12877046-12877065 Msa0838490:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_4:12877112-12877131 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_4:12877291-12877310 Msa0838490:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_4:12877292-12877311 Msa0838490:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_4:12877337-12877356 Msa0838490:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_4:12877339-12877358 Msa0838490:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_4:12877369-12877388 Msa0838490:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_4:12877544-12877563 Msa0838490:intron 35.0%
GAATCAGGATGAATTATGGT+TGG + chr5_4:12877740-12877759 None:intergenic 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_4:12877813-12877832 Msa0838490:CDS 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_4:12877824-12877843 Msa0838490:CDS 35.0%
ACATCATAGAAAAGGTTGTG+GGG + chr5_4:12878180-12878199 None:intergenic 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_4:12878290-12878309 Msa0838490:CDS 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_4:12878459-12878478 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_4:12878478-12878497 Msa0838490:five_prime_UTR 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_4:12878551-12878570 None:intergenic 35.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_4:12876804-12876823 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_4:12877046-12877065 Msa0838490:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_4:12877112-12877131 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_4:12877291-12877310 Msa0838490:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_4:12877292-12877311 Msa0838490:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_4:12877337-12877356 Msa0838490:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_4:12877339-12877358 Msa0838490:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_4:12877369-12877388 Msa0838490:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_4:12877544-12877563 Msa0838490:intron 35.0%
GAATCAGGATGAATTATGGT+TGG + chr5_4:12877740-12877759 None:intergenic 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_4:12877813-12877832 Msa0838490:CDS 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_4:12877824-12877843 Msa0838490:CDS 35.0%
ACATCATAGAAAAGGTTGTG+GGG + chr5_4:12878180-12878199 None:intergenic 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_4:12878290-12878309 Msa0838490:CDS 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_4:12878459-12878478 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_4:12878478-12878497 Msa0838490:five_prime_UTR 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_4:12878551-12878570 None:intergenic 35.0%
GCAACAAAAAGTCTATCCAC+TGG - chr5_4:12876589-12876608 Msa0838490:three_prime_UTR 40.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_4:12876956-12876975 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_4:12876985-12877004 None:intergenic 40.0%
TTAAGCTCAGTTCAAACCTC+AGG + chr5_4:12877285-12877304 None:intergenic 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_4:12877352-12877371 Msa0838490:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_4:12877708-12877727 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_4:12877717-12877736 None:intergenic 40.0%
GTCAGTTGTTCAACTGAAGT+TGG + chr5_4:12877798-12877817 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_4:12877844-12877863 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_4:12878370-12878389 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_4:12878421-12878440 Msa0838490:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_4:12878433-12878452 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_4:12878443-12878462 Msa0838490:CDS 40.0%
GCAACAAAAAGTCTATCCAC+TGG - chr5_4:12876589-12876608 Msa0838490:three_prime_UTR 40.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_4:12876956-12876975 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_4:12876985-12877004 None:intergenic 40.0%
TTAAGCTCAGTTCAAACCTC+AGG + chr5_4:12877285-12877304 None:intergenic 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_4:12877352-12877371 Msa0838490:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_4:12877708-12877727 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_4:12877717-12877736 None:intergenic 40.0%
GTCAGTTGTTCAACTGAAGT+TGG + chr5_4:12877798-12877817 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_4:12877844-12877863 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_4:12878370-12878389 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_4:12878421-12878440 Msa0838490:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_4:12878433-12878452 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_4:12878443-12878462 Msa0838490:CDS 40.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_4:12876523-12876542 Msa0838490:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_4:12876531-12876550 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_4:12876532-12876551 None:intergenic 45.0%
TTTCTTCCACACTACACCAG+TGG + chr5_4:12876608-12876627 None:intergenic 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_4:12876607-12876626 Msa0838490:three_prime_UTR 45.0%
GTGTAGTGTGGAAGAAAGGT+AGG - chr5_4:12876611-12876630 Msa0838490:three_prime_UTR 45.0%
AGTGTGGAAGAAAGGTAGGT+TGG - chr5_4:12876615-12876634 Msa0838490:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_4:12876751-12876770 Msa0838490:CDS 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_4:12876752-12876771 Msa0838490:CDS 45.0%
!! ATTCGGAATTTTGGAAGCGG+AGG + chr5_4:12876801-12876820 None:intergenic 45.0%
GTCACACTCTCTTCTGTTCT+TGG + chr5_4:12876834-12876853 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_4:12876856-12876875 Msa0838490:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_4:12876859-12876878 Msa0838490:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_4:12876871-12876890 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_4:12876875-12876894 Msa0838490:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_4:12876881-12876900 Msa0838490:intron 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_4:12876905-12876924 Msa0838490:intron 45.0%
!! TATAGGAGCAAGAGTACCTG+AGG - chr5_4:12877266-12877285 Msa0838490:intron 45.0%
GAATGAGGGTGATGAACATG+AGG - chr5_4:12877306-12877325 Msa0838490:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_4:12877401-12877420 Msa0838490:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_4:12877564-12877583 None:intergenic 45.0%
! AGTTGGTTCAACCGGACTAT+TGG + chr5_4:12877700-12877719 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_4:12877707-12877726 Msa0838490:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_4:12878356-12878375 Msa0838490:CDS 45.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_4:12876523-12876542 Msa0838490:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_4:12876531-12876550 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_4:12876532-12876551 None:intergenic 45.0%
TTTCTTCCACACTACACCAG+TGG + chr5_4:12876608-12876627 None:intergenic 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_4:12876607-12876626 Msa0838490:three_prime_UTR 45.0%
GTGTAGTGTGGAAGAAAGGT+AGG - chr5_4:12876611-12876630 Msa0838490:three_prime_UTR 45.0%
AGTGTGGAAGAAAGGTAGGT+TGG - chr5_4:12876615-12876634 Msa0838490:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_4:12876751-12876770 Msa0838490:CDS 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_4:12876752-12876771 Msa0838490:CDS 45.0%
!! ATTCGGAATTTTGGAAGCGG+AGG + chr5_4:12876801-12876820 None:intergenic 45.0%
GTCACACTCTCTTCTGTTCT+TGG + chr5_4:12876834-12876853 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_4:12876856-12876875 Msa0838490:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_4:12876859-12876878 Msa0838490:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_4:12876871-12876890 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_4:12876875-12876894 Msa0838490:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_4:12876881-12876900 Msa0838490:intron 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_4:12876905-12876924 Msa0838490:intron 45.0%
!! TATAGGAGCAAGAGTACCTG+AGG - chr5_4:12877266-12877285 Msa0838490:intron 45.0%
GAATGAGGGTGATGAACATG+AGG - chr5_4:12877306-12877325 Msa0838490:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_4:12877401-12877420 Msa0838490:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_4:12877564-12877583 None:intergenic 45.0%
! AGTTGGTTCAACCGGACTAT+TGG + chr5_4:12877700-12877719 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_4:12877707-12877726 Msa0838490:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_4:12878356-12878375 Msa0838490:CDS 45.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_4:12876522-12876541 Msa0838490:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_4:12876524-12876543 Msa0838490:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_4:12876530-12876549 None:intergenic 50.0%
GTCTATCCACTGGTGTAGTG+TGG - chr5_4:12876599-12876618 Msa0838490:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_4:12876766-12876785 None:intergenic 50.0%
!! AATTTTGGAAGCGGAGGAGG+AGG + chr5_4:12876795-12876814 None:intergenic 50.0%
GAATGAATCGAGCTGAGCCA+TGG + chr5_4:12877662-12877681 None:intergenic 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_4:12878350-12878369 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_4:12878359-12878378 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_4:12878367-12878386 None:intergenic 50.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_4:12876522-12876541 Msa0838490:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_4:12876524-12876543 Msa0838490:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_4:12876530-12876549 None:intergenic 50.0%
GTCTATCCACTGGTGTAGTG+TGG - chr5_4:12876599-12876618 Msa0838490:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_4:12876766-12876785 None:intergenic 50.0%
!! AATTTTGGAAGCGGAGGAGG+AGG + chr5_4:12876795-12876814 None:intergenic 50.0%
GAATGAATCGAGCTGAGCCA+TGG + chr5_4:12877662-12877681 None:intergenic 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_4:12878350-12878369 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_4:12878359-12878378 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_4:12878367-12878386 None:intergenic 50.0%
!! CGGAATTTTGGAAGCGGAGG+AGG + chr5_4:12876798-12876817 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_4:12876914-12876933 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_4:12877387-12877406 Msa0838490:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_4:12877388-12877407 Msa0838490:intron 55.0%
CGAATTGGTTGAACCGGCCA+TGG - chr5_4:12877642-12877661 Msa0838490:intron 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_4:12878342-12878361 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_4:12878339-12878358 Msa0838490:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_4:12878346-12878365 None:intergenic 55.0%
!! CGGAATTTTGGAAGCGGAGG+AGG + chr5_4:12876798-12876817 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_4:12876914-12876933 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_4:12877387-12877406 Msa0838490:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_4:12877388-12877407 Msa0838490:intron 55.0%
CGAATTGGTTGAACCGGCCA+TGG - chr5_4:12877642-12877661 Msa0838490:intron 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_4:12878342-12878361 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_4:12878339-12878358 Msa0838490:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_4:12878346-12878365 None:intergenic 55.0%
TTTGGAAGCGGAGGAGGAGG+AGG + chr5_4:12876792-12876811 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_4:12876944-12876963 None:intergenic 60.0%
GAATCGAGCTGAGCCATGGC+CGG + chr5_4:12877658-12877677 None:intergenic 60.0%
TTTGGAAGCGGAGGAGGAGG+AGG + chr5_4:12876792-12876811 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_4:12876944-12876963 None:intergenic 60.0%
GAATCGAGCTGAGCCATGGC+CGG + chr5_4:12877658-12877677 None:intergenic 60.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_4:12876938-12876957 Msa0838490:intron 65.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_4:12876938-12876957 Msa0838490:intron 65.0%
AGCGGAGGAGGAGGAGGTGG+TGG + chr5_4:12876786-12876805 None:intergenic 70.0%
GGAAGCGGAGGAGGAGGAGG+TGG + chr5_4:12876789-12876808 None:intergenic 70.0%
AGCGGAGGAGGAGGAGGTGG+TGG + chr5_4:12876786-12876805 None:intergenic 70.0%
GGAAGCGGAGGAGGAGGAGG+TGG + chr5_4:12876789-12876808 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 12876520 12878684 12876520 ID=Msa0838490;Name=Msa0838490
chr5_4 mRNA 12876520 12878684 12876520 ID=Msa0838490-mRNA-1;Parent=Msa0838490;Name=Msa0838490-mRNA-1;_AED=0.13;_eAED=0.13;_QI=218|1|1|1|1|1|3|231|172
chr5_4 exon 12876520 12876895 12876520 ID=Msa0838490-mRNA-1:exon:5712;Parent=Msa0838490-mRNA-1
chr5_4 exon 12877782 12877933 12877782 ID=Msa0838490-mRNA-1:exon:5711;Parent=Msa0838490-mRNA-1
chr5_4 exon 12878245 12878684 12878245 ID=Msa0838490-mRNA-1:exon:5710;Parent=Msa0838490-mRNA-1
chr5_4 five_prime_UTR 12878467 12878684 12878467 ID=Msa0838490-mRNA-1:five_prime_utr;Parent=Msa0838490-mRNA-1
chr5_4 CDS 12878245 12878466 12878245 ID=Msa0838490-mRNA-1:cds;Parent=Msa0838490-mRNA-1
chr5_4 CDS 12877782 12877933 12877782 ID=Msa0838490-mRNA-1:cds;Parent=Msa0838490-mRNA-1
chr5_4 CDS 12876751 12876895 12876751 ID=Msa0838490-mRNA-1:cds;Parent=Msa0838490-mRNA-1
chr5_4 three_prime_UTR 12876520 12876750 12876520 ID=Msa0838490-mRNA-1:three_prime_utr;Parent=Msa0838490-mRNA-1
Gene Sequence

>Msa0838490

ATGAAGCAAAGAAAGGAACAAGCAACCGTTTCAAGGGCAGCAACACCACCACCTCCTCCTCCTCCGCTTCCAAAATTCCGAATCATCTCAAAACCAAGAACAGAAGAGAGTGTGACAAAGCGCGAAATTGCCAAGTTTTGGAGGCAAAAGCGAATTGTGGAAGAGGATCATCTCCTTGCTGCTATCAAGGCTGCAGCAAGACTCCGTGCGCGCAACCTCACGGAGCAAGAGTACCTGAGGTTTGAACTGAGCTTAAAGAATGAGGGTGATGAACATGAGGCCAATAAAGAGACTATCAATTTGGTTGTAGGGGCAAATTATTGTGGCAATAAAGTTGATGAGGACAAAGAAGTGCGCGTGGGAATAAAAGATTGGTGGACTAAAAGCAAGTATGCATATTTGAACCAACCAGCCTTAGCTTCCATGGATCCTCCTAAGAAACGGACCTCTACTTATGTTCCACATATTCTTTCTTACAAGCATAAGGCTTTGTATCCTACTGCTATTGGTGTCTTTTGA
Protein sequence

>Msa0838490

MKQRKEQATVSRAATPPPPPPPLPKFRIISKPRTEESVTKREIAKFWRQKRIVEEDHLLAAIKAAARLRARNLTEQEYLRFELSLKNEGDEHEANKETINLVVGANYCGNKVDEDKEVRVGIKDWWTKSKYAYLNQPALASMDPPKKRTSTYVPHILSYKHKALYPTAIGVF*