AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0800490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800490 MtrunA17_Chr5g0406071 92.442 172 12 1 1 172 1 171 3.49e-108 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800490 AT2G17710.1 50.360 139 43 3 36 172 38 152 2.35e-38 129

Find 65 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCCCATGTGTGTTATT+TGG 0.092661 5_3:-13878235 Msa0800490:three_prime_UTR
GTCACACTCTCTTCTGTTCT+TGG 0.123272 5_3:+13879948 None:intergenic
TATGTCAACTGGTCAATTTC+TGG 0.137128 5_3:-13878299 Msa0800490:three_prime_UTR
CAAATTGATAGTCTCTTTAT+TGG 0.141487 5_3:+13879451 None:intergenic
ATCACCAGTAATAGAATTTC+TGG 0.152523 5_3:-13878118 Msa0800490:three_prime_UTR
TTTGAGATGATTCGGAATTT+TGG 0.208841 5_3:+13879972 None:intergenic
GTTCTCCCCACGCAAGTAAA+AGG 0.227857 5_3:-13880256 Msa0800490:five_prime_UTR
AAATCCCATGTGTGTTATTT+GGG 0.285292 5_3:-13878234 Msa0800490:three_prime_UTR
TAAGTAGAGGTCCGTTTCTT+AGG 0.285599 5_3:+13878410 None:intergenic
AGCGCGAAATTGCCAAGTTT+TGG 0.285984 5_3:-13879923 Msa0800490:CDS
CAATAAAGAGACTATCAATT+TGG 0.299593 5_3:-13879450 Msa0800490:CDS
GCTTTGTATCCTACTGCTAT+TGG 0.336710 5_3:-13878356 Msa0800490:CDS
ATGTCCAGAAATTCTATTAC+TGG 0.344567 5_3:+13878114 None:intergenic
CTAAAATAGATTTGAGATTA+CGG 0.369925 5_3:+13880123 None:intergenic
ATAATATCGAGTGCCTCTTA+TGG 0.373495 5_3:+13878321 None:intergenic
ATTGTTACATTTGTTACAAC+TGG 0.377760 5_3:-13878205 Msa0800490:three_prime_UTR
GAGACTATCAATTTGGTTGT+AGG 0.381164 5_3:-13879443 Msa0800490:CDS
AGCGGAGGAGGAGGAGGTGG+TGG 0.385556 5_3:+13879996 None:intergenic
TTTGGAAGCGGAGGAGGAGG+AGG 0.392926 5_3:+13879990 None:intergenic
GGAAGCGGAGGAGGAGGAGG+TGG 0.398218 5_3:+13879993 None:intergenic
TATTCTTTCTTACAAGCATA+AGG 0.399281 5_3:-13878378 Msa0800490:CDS
AGACTATCAATTTGGTTGTA+GGG 0.440855 5_3:-13879442 Msa0800490:CDS
TCATCTCCTTGCTGCTATCA+AGG 0.456611 5_3:-13879874 Msa0800490:CDS
TTCTCCCCACGCAAGTAAAA+GGG 0.456820 5_3:-13880255 Msa0800490:five_prime_UTR
ACTCGATATTATATGTCAAC+TGG 0.464577 5_3:-13878310 Msa0800490:three_prime_UTR
AGGAACAAGCAACCGTTTCA+AGG 0.465570 5_3:-13880028 Msa0800490:CDS
CCAACCAGCCTTAGCTTCCA+TGG 0.474612 5_3:-13878438 Msa0800490:CDS
TGTTGAAATGAAGCAAAGAA+AGG 0.479451 5_3:-13880048 Msa0800490:exon
AATCAATTCTTCTAATAGAA+AGG 0.479596 5_3:+13878089 None:intergenic
TGAACTGAGCTTAAAGAATG+AGG 0.483526 5_3:-13879489 Msa0800490:CDS
GACTATCAATTTGGTTGTAG+GGG 0.511564 5_3:-13879441 Msa0800490:CDS
ACTGGTGTAGTGTGGAAGAA+AGG 0.518191 5_3:-13880171 Msa0800490:five_prime_UTR
AGGAGGATCCATGGAAGCTA+AGG 0.520566 5_3:+13878430 None:intergenic
TTACCGTGAGGTTGCGCGCA+CGG 0.533446 5_3:+13879838 None:intergenic
CCGTTTCTTAGGAGGATCCA+TGG 0.535644 5_3:+13878421 None:intergenic
TGGTGTTGCTGCCCTTGAAA+CGG 0.539688 5_3:+13880016 None:intergenic
GTAGAGGTCCGTTTCTTAGG+AGG 0.541548 5_3:+13878413 None:intergenic
TGCGCGTGGGAATAAAAGAT+TGG 0.546641 5_3:-13879379 Msa0800490:intron
CCATGGATCCTCCTAAGAAA+CGG 0.550809 5_3:-13878421 Msa0800490:CDS
CTTATCCCAAATAACACACA+TGG 0.550961 5_3:+13878229 None:intergenic
TAGTGTGGAAGAAAGGAGGT+TGG 0.553309 5_3:-13880164 Msa0800490:five_prime_UTR
TGCTTGTAAGAAAGAATATG+TGG 0.555711 5_3:+13878383 None:intergenic
GAACTGAGCTTAAAGAATGA+GGG 0.561286 5_3:-13879488 Msa0800490:CDS
GCAAAAGCGAATTGTGGAAG+AGG 0.567524 5_3:-13879898 Msa0800490:CDS
TGCAAATGGAATTTCTTAGT+CGG 0.570973 5_3:+13880209 None:intergenic
CCATGGAAGCTAAGGCTGGT+TGG 0.571317 5_3:+13878438 None:intergenic
GGAACAAGCAACCGTTTCAA+GGG 0.579284 5_3:-13880027 Msa0800490:CDS
GTCTATCTACTGGTGTAGTG+TGG 0.586452 5_3:-13880179 Msa0800490:five_prime_UTR
GGATCCATGGAAGCTAAGGC+TGG 0.586903 5_3:+13878434 None:intergenic
ACTCCGTGCGCGCAACCTCA+CGG 0.592771 5_3:-13879841 Msa0800490:intron
TATAGGAGCAAGAGTATCTG+AGG 0.594659 5_3:-13879514 Msa0800490:intron
CAAAAGACACCAATAGCAGT+AGG 0.596240 5_3:+13878347 None:intergenic
TTGTGGCAATAAAGTTGATG+AGG 0.597067 5_3:-13879411 Msa0800490:CDS
GAATGAGGGTGATGAAGATG+AGG 0.611006 5_3:-13879474 Msa0800490:CDS
GTTGTAGGGGCAAATTATTG+TGG 0.611103 5_3:-13879428 Msa0800490:CDS
CTGCAGCCTTGATAGCAGCA+AGG 0.633031 5_3:+13879868 None:intergenic
AATATGTGGAACATAAGTAG+AGG 0.646153 5_3:+13878397 None:intergenic
TTGGAGGCAAAAGCGAATTG+TGG 0.647122 5_3:-13879904 Msa0800490:CDS
TGAGGACAAAGAAGTGCGCG+TGG 0.647667 5_3:-13879393 Msa0800490:CDS
AACAAATGAAACATTAACAA+GGG 0.647858 5_3:+13878262 None:intergenic
TAACAAATGAAACATTAACA+AGG 0.656419 5_3:+13878261 None:intergenic
TCTCCCCACGCAAGTAAAAG+GGG 0.658819 5_3:-13880254 Msa0800490:five_prime_UTR
GGTGTAGTGTGGAAGAAAGG+AGG 0.670095 5_3:-13880168 Msa0800490:five_prime_UTR
TTATCCCAAATAACACACAT+GGG 0.689145 5_3:+13878230 None:intergenic
GAGGACAAAGAAGTGCGCGT+GGG 0.694290 5_3:-13879392 Msa0800490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATCATTTTATTTTTTATG+CGG + chr5_3:13878597-13878616 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_3:13879419-13879438 Msa0800490:CDS 10.0%
!!! TTTTTTTTTTTAATGAGAAT+TGG + chr5_3:13879530-13879549 None:intergenic 10.0%
!!! AAATCATTTTATTTTTTATG+CGG + chr5_3:13878597-13878616 None:intergenic 10.0%
!! TTTAAAAGTTAAAATATATG+AGG - chr5_3:13879419-13879438 Msa0800490:CDS 10.0%
!!! TTTTTTTTTTTAATGAGAAT+TGG + chr5_3:13879530-13879549 None:intergenic 10.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_3:13878536-13878555 Msa0800490:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_3:13878658-13878677 None:intergenic 15.0%
!!! ATTAACATATATTTTTCATG+TGG - chr5_3:13878683-13878702 Msa0800490:intron 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_3:13878768-13878787 Msa0800490:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_3:13879008-13879027 Msa0800490:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_3:13879146-13879165 Msa0800490:intron 15.0%
!! AATTGAAATTTAGAAGATTA+AGG - chr5_3:13879501-13879520 Msa0800490:CDS 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_3:13879596-13879615 Msa0800490:intron 15.0%
!! GTCTATATAAATCAAATATA+AGG - chr5_3:13878536-13878555 Msa0800490:intron 15.0%
!!! AATTTATAAACAATTTTGCT+TGG + chr5_3:13878658-13878677 None:intergenic 15.0%
!!! ATTAACATATATTTTTCATG+TGG - chr5_3:13878683-13878702 Msa0800490:intron 15.0%
!!! ATTAATTGCTATTTGATTAT+AGG - chr5_3:13878768-13878787 Msa0800490:intron 15.0%
!!! ATTTTAACAAGATTTGTAAT+AGG - chr5_3:13879008-13879027 Msa0800490:intron 15.0%
!! AATAAAACATAAAAACGAAT+TGG - chr5_3:13879146-13879165 Msa0800490:intron 15.0%
!! AATTGAAATTTAGAAGATTA+AGG - chr5_3:13879501-13879520 Msa0800490:CDS 15.0%
!!! GAGACTAAAATTATTTTTTT+TGG - chr5_3:13879596-13879615 Msa0800490:intron 15.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_3:13878179-13878198 None:intergenic 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_3:13879093-13879112 None:intergenic 20.0%
!! ACAAGATAACATCATATAAA+AGG + chr5_3:13879710-13879729 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_3:13879746-13879765 Msa0800490:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_3:13880040-13880059 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_3:13880041-13880060 None:intergenic 20.0%
!! AATCAATTCTTCTAATAGAA+AGG + chr5_3:13880213-13880232 None:intergenic 20.0%
!!! CTAAAATAGATTTGAGATTA+CGG + chr5_3:13878179-13878198 None:intergenic 20.0%
!!! TATTTATTTGTTAGTCATCA+AGG + chr5_3:13879093-13879112 None:intergenic 20.0%
!! ACAAGATAACATCATATAAA+AGG + chr5_3:13879710-13879729 None:intergenic 20.0%
!!! CATAAATGAAACCATTTTAA+AGG - chr5_3:13879746-13879765 Msa0800490:intron 20.0%
!! AACAAATGAAACATTAACAA+GGG + chr5_3:13880040-13880059 None:intergenic 20.0%
!! TAACAAATGAAACATTAACA+AGG + chr5_3:13880041-13880060 None:intergenic 20.0%
!! AATCAATTCTTCTAATAGAA+AGG + chr5_3:13880213-13880232 None:intergenic 20.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_3:13878654-13878673 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_3:13878851-13878870 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_3:13878849-13878868 Msa0800490:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_3:13878932-13878951 Msa0800490:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_3:13878965-13878984 Msa0800490:intron 25.0%
!!! TTGATTTAGTTCTAATAGTC+CGG - chr5_3:13879205-13879224 Msa0800490:intron 25.0%
! GATTGAATCATGATGAATTA+TGG + chr5_3:13879263-13879282 None:intergenic 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_3:13879297-13879316 None:intergenic 25.0%
!!! CTGTCTAGTTTCATTTTTTT+TGG + chr5_3:13879445-13879464 None:intergenic 25.0%
! AACATCATATAAAAGGTTGT+GGG + chr5_3:13879703-13879722 None:intergenic 25.0%
! TAACATCATATAAAAGGTTG+TGG + chr5_3:13879704-13879723 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_3:13879760-13879779 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_3:13879921-13879940 Msa0800490:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_3:13880094-13880113 Msa0800490:five_prime_UTR 25.0%
!! TATAAACAATTTTGCTTGGA+TGG + chr5_3:13878654-13878673 None:intergenic 25.0%
!! CAAATTGATAGTCTCTTTAT+TGG + chr5_3:13878851-13878870 None:intergenic 25.0%
! CAATAAAGAGACTATCAATT+TGG - chr5_3:13878849-13878868 Msa0800490:intron 25.0%
!! TAAAAGATTGGTTAGTATCT+TGG - chr5_3:13878932-13878951 Msa0800490:intron 25.0%
! CATCAAGCTATTAAATTGTT+TGG - chr5_3:13878965-13878984 Msa0800490:intron 25.0%
!!! TTGATTTAGTTCTAATAGTC+CGG - chr5_3:13879205-13879224 Msa0800490:intron 25.0%
! GATTGAATCATGATGAATTA+TGG + chr5_3:13879263-13879282 None:intergenic 25.0%
!!! TGGTTGTACTCTTTTAAAAT+CGG + chr5_3:13879297-13879316 None:intergenic 25.0%
!!! CTGTCTAGTTTCATTTTTTT+TGG + chr5_3:13879445-13879464 None:intergenic 25.0%
! AACATCATATAAAAGGTTGT+GGG + chr5_3:13879703-13879722 None:intergenic 25.0%
! TAACATCATATAAAAGGTTG+TGG + chr5_3:13879704-13879723 None:intergenic 25.0%
!! AACTACAAACTCCTTTAAAA+TGG + chr5_3:13879760-13879779 None:intergenic 25.0%
! TATTCTTTCTTACAAGCATA+AGG - chr5_3:13879921-13879940 Msa0800490:CDS 25.0%
!! ATTGTTACATTTGTTACAAC+TGG - chr5_3:13880094-13880113 Msa0800490:five_prime_UTR 25.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_3:13878093-13878112 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_3:13878107-13878126 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_3:13878251-13878270 Msa0800490:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_3:13878330-13878349 None:intergenic 30.0%
!!! TTCTTGGTTTTGAGATGATT+CGG + chr5_3:13878338-13878357 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_3:13878857-13878876 Msa0800490:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_3:13878971-13878990 Msa0800490:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_3:13879155-13879174 Msa0800490:intron 30.0%
GAATCATGATGAATTATGGT+TGG + chr5_3:13879259-13879278 None:intergenic 30.0%
ATTATCACTGAGATTAAAGC+AGG + chr5_3:13879478-13879497 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_3:13879563-13879582 Msa0800490:intron 30.0%
ACATCATATAAAAGGTTGTG+GGG + chr5_3:13879702-13879721 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_3:13879809-13879828 Msa0800490:intron 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_3:13879905-13879924 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_3:13879919-13879938 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_3:13879989-13880008 Msa0800490:CDS 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_3:13880064-13880083 Msa0800490:five_prime_UTR 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_3:13880065-13880084 Msa0800490:five_prime_UTR 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_3:13880072-13880091 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_3:13880151-13880170 Msa0800490:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_3:13880181-13880200 Msa0800490:five_prime_UTR 30.0%
ATGTCCAGAAATTCTATTAC+TGG + chr5_3:13880188-13880207 None:intergenic 30.0%
TGCAAATGGAATTTCTTAGT+CGG + chr5_3:13878093-13878112 None:intergenic 30.0%
! GATAGACTTTTTGTTGCAAA+TGG + chr5_3:13878107-13878126 None:intergenic 30.0%
TGTTGAAATGAAGCAAAGAA+AGG - chr5_3:13878251-13878270 Msa0800490:three_prime_UTR 30.0%
!! TTTGAGATGATTCGGAATTT+TGG + chr5_3:13878330-13878349 None:intergenic 30.0%
!!! TTCTTGGTTTTGAGATGATT+CGG + chr5_3:13878338-13878357 None:intergenic 30.0%
AGACTATCAATTTGGTTGTA+GGG - chr5_3:13878857-13878876 Msa0800490:intron 30.0%
GCTATTAAATTGTTTGGACA+AGG - chr5_3:13878971-13878990 Msa0800490:intron 30.0%
TAAAAACGAATTGGTTGAAC+CGG - chr5_3:13879155-13879174 Msa0800490:intron 30.0%
GAATCATGATGAATTATGGT+TGG + chr5_3:13879259-13879278 None:intergenic 30.0%
ATTATCACTGAGATTAAAGC+AGG + chr5_3:13879478-13879497 None:intergenic 30.0%
GAGTGAAAGTAAAGAAATAC+AGG - chr5_3:13879563-13879582 Msa0800490:intron 30.0%
ACATCATATAAAAGGTTGTG+GGG + chr5_3:13879702-13879721 None:intergenic 30.0%
!!! GTATTAGTTTTGTCATGTAC+AGG - chr5_3:13879809-13879828 Msa0800490:intron 30.0%
AATATGTGGAACATAAGTAG+AGG + chr5_3:13879905-13879924 None:intergenic 30.0%
TGCTTGTAAGAAAGAATATG+TGG + chr5_3:13879919-13879938 None:intergenic 30.0%
ACTCGATATTATATGTCAAC+TGG - chr5_3:13879989-13880008 Msa0800490:CDS 30.0%
! AAAATCCCATGTGTGTTATT+TGG - chr5_3:13880064-13880083 Msa0800490:five_prime_UTR 30.0%
!! AAATCCCATGTGTGTTATTT+GGG - chr5_3:13880065-13880084 Msa0800490:five_prime_UTR 30.0%
TTATCCCAAATAACACACAT+GGG + chr5_3:13880072-13880091 None:intergenic 30.0%
!!! TGACTTGCTATTCTATTTTC+TGG - chr5_3:13880151-13880170 Msa0800490:five_prime_UTR 30.0%
ATCACCAGTAATAGAATTTC+TGG - chr5_3:13880181-13880200 Msa0800490:five_prime_UTR 30.0%
ATGTCCAGAAATTCTATTAC+TGG + chr5_3:13880188-13880207 None:intergenic 30.0%
GCAACAAAAAGTCTATCTAC+TGG - chr5_3:13878110-13878129 Msa0800490:three_prime_UTR 35.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_3:13878324-13878343 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_3:13878566-13878585 Msa0800490:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_3:13878631-13878650 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_3:13878810-13878829 Msa0800490:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_3:13878811-13878830 Msa0800490:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_3:13878856-13878875 Msa0800490:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_3:13878858-13878877 Msa0800490:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_3:13878888-13878907 Msa0800490:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_3:13879063-13879082 Msa0800490:intron 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_3:13879332-13879351 Msa0800490:intron 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_3:13879343-13879362 Msa0800490:intron 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_3:13879812-13879831 Msa0800490:intron 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_3:13879981-13880000 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_3:13880000-13880019 Msa0800490:CDS 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_3:13880073-13880092 None:intergenic 35.0%
GCAACAAAAAGTCTATCTAC+TGG - chr5_3:13878110-13878129 Msa0800490:three_prime_UTR 35.0%
!! ATGATTCGGAATTTTGGAAG+CGG + chr5_3:13878324-13878343 None:intergenic 35.0%
GTGTGCATGTGTATGTATTA+TGG - chr5_3:13878566-13878585 Msa0800490:intron 35.0%
AGCTACAAAGATGATCTAGA+AGG + chr5_3:13878631-13878650 None:intergenic 35.0%
TGAACTGAGCTTAAAGAATG+AGG - chr5_3:13878810-13878829 Msa0800490:intron 35.0%
GAACTGAGCTTAAAGAATGA+GGG - chr5_3:13878811-13878830 Msa0800490:intron 35.0%
GAGACTATCAATTTGGTTGT+AGG - chr5_3:13878856-13878875 Msa0800490:intron 35.0%
GACTATCAATTTGGTTGTAG+GGG - chr5_3:13878858-13878877 Msa0800490:intron 35.0%
!! TTGTGGCAATAAAGTTGATG+AGG - chr5_3:13878888-13878907 Msa0800490:intron 35.0%
GAGATCAATAGTTTGAACCA+CGG - chr5_3:13879063-13879082 Msa0800490:intron 35.0%
CTGACAGATTGATTGATTAC+TGG - chr5_3:13879332-13879351 Msa0800490:intron 35.0%
! ATTGATTACTGGTTAAACCG+AGG - chr5_3:13879343-13879362 Msa0800490:intron 35.0%
!!! TTAGTTTTGTCATGTACAGG+TGG - chr5_3:13879812-13879831 Msa0800490:intron 35.0%
ATAATATCGAGTGCCTCTTA+TGG + chr5_3:13879981-13880000 None:intergenic 35.0%
TATGTCAACTGGTCAATTTC+TGG - chr5_3:13880000-13880019 Msa0800490:CDS 35.0%
CTTATCCCAAATAACACACA+TGG + chr5_3:13880073-13880092 None:intergenic 35.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_3:13878476-13878495 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_3:13878505-13878524 None:intergenic 40.0%
!! TATAGGAGCAAGAGTATCTG+AGG - chr5_3:13878785-13878804 Msa0800490:intron 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_3:13878871-13878890 Msa0800490:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_3:13879227-13879246 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_3:13879236-13879255 None:intergenic 40.0%
GTCAGTTGTTCAACTGTAGT+TGG + chr5_3:13879317-13879336 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_3:13879363-13879382 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_3:13879892-13879911 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_3:13879943-13879962 Msa0800490:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_3:13879955-13879974 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_3:13879965-13879984 Msa0800490:CDS 40.0%
! ACTATATGTCACTTACCGTG+AGG + chr5_3:13878476-13878495 None:intergenic 40.0%
ATTATAGATAGCTGCTGCAG+AGG + chr5_3:13878505-13878524 None:intergenic 40.0%
!! TATAGGAGCAAGAGTATCTG+AGG - chr5_3:13878785-13878804 Msa0800490:intron 40.0%
GTTGTAGGGGCAAATTATTG+TGG - chr5_3:13878871-13878890 Msa0800490:intron 40.0%
! AATCTGTCAGTTGGTTCAAC+CGG + chr5_3:13879227-13879246 None:intergenic 40.0%
TTCATTGCCAATCTGTCAGT+TGG + chr5_3:13879236-13879255 None:intergenic 40.0%
GTCAGTTGTTCAACTGTAGT+TGG + chr5_3:13879317-13879336 None:intergenic 40.0%
TTCATCATCGAAAGAGACCT+CGG + chr5_3:13879363-13879382 None:intergenic 40.0%
TAAGTAGAGGTCCGTTTCTT+AGG + chr5_3:13879892-13879911 None:intergenic 40.0%
GCTTTGTATCCTACTGCTAT+TGG - chr5_3:13879943-13879962 Msa0800490:CDS 40.0%
CAAAAGACACCAATAGCAGT+AGG + chr5_3:13879955-13879974 None:intergenic 40.0%
!!! GTGTCTTTTGAAGCCATAAG+AGG - chr5_3:13879965-13879984 Msa0800490:CDS 40.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_3:13878044-13878063 Msa0800490:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_3:13878052-13878071 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_3:13878053-13878072 None:intergenic 45.0%
GTCTATCTACTGGTGTAGTG+TGG - chr5_3:13878120-13878139 Msa0800490:three_prime_UTR 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_3:13878128-13878147 Msa0800490:three_prime_UTR 45.0%
TAGTGTGGAAGAAAGGAGGT+TGG - chr5_3:13878135-13878154 Msa0800490:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_3:13878271-13878290 Msa0800490:three_prime_UTR 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_3:13878272-13878291 Msa0800490:three_prime_UTR 45.0%
!! ATTCGGAATTTTGGAAGCGG+AGG + chr5_3:13878321-13878340 None:intergenic 45.0%
GTCACACTCTCTTCTGTTCT+TGG + chr5_3:13878354-13878373 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_3:13878376-13878395 Msa0800490:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_3:13878379-13878398 Msa0800490:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_3:13878391-13878410 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_3:13878395-13878414 Msa0800490:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_3:13878401-13878420 Msa0800490:CDS 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_3:13878425-13878444 Msa0800490:CDS 45.0%
GAATGAGGGTGATGAAGATG+AGG - chr5_3:13878825-13878844 Msa0800490:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_3:13878920-13878939 Msa0800490:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_3:13879083-13879102 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_3:13879226-13879245 Msa0800490:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_3:13879878-13879897 Msa0800490:CDS 45.0%
TTCTCCCCACGCAAGTAAAA+GGG - chr5_3:13878044-13878063 Msa0800490:three_prime_UTR 45.0%
! ATAGCCCCTTTTACTTGCGT+GGG + chr5_3:13878052-13878071 None:intergenic 45.0%
! TATAGCCCCTTTTACTTGCG+TGG + chr5_3:13878053-13878072 None:intergenic 45.0%
GTCTATCTACTGGTGTAGTG+TGG - chr5_3:13878120-13878139 Msa0800490:three_prime_UTR 45.0%
ACTGGTGTAGTGTGGAAGAA+AGG - chr5_3:13878128-13878147 Msa0800490:three_prime_UTR 45.0%
TAGTGTGGAAGAAAGGAGGT+TGG - chr5_3:13878135-13878154 Msa0800490:three_prime_UTR 45.0%
AGGAACAAGCAACCGTTTCA+AGG - chr5_3:13878271-13878290 Msa0800490:three_prime_UTR 45.0%
GGAACAAGCAACCGTTTCAA+GGG - chr5_3:13878272-13878291 Msa0800490:three_prime_UTR 45.0%
!! ATTCGGAATTTTGGAAGCGG+AGG + chr5_3:13878321-13878340 None:intergenic 45.0%
GTCACACTCTCTTCTGTTCT+TGG + chr5_3:13878354-13878373 None:intergenic 45.0%
! AGCGCGAAATTGCCAAGTTT+TGG - chr5_3:13878376-13878395 Msa0800490:CDS 45.0%
!! GCGAAATTGCCAAGTTTTGG+AGG - chr5_3:13878379-13878398 Msa0800490:CDS 45.0%
!! TCGCTTTTGCCTCCAAAACT+TGG + chr5_3:13878391-13878410 None:intergenic 45.0%
TTGGAGGCAAAAGCGAATTG+TGG - chr5_3:13878395-13878414 Msa0800490:CDS 45.0%
GCAAAAGCGAATTGTGGAAG+AGG - chr5_3:13878401-13878420 Msa0800490:CDS 45.0%
TCATCTCCTTGCTGCTATCA+AGG - chr5_3:13878425-13878444 Msa0800490:CDS 45.0%
GAATGAGGGTGATGAAGATG+AGG - chr5_3:13878825-13878844 Msa0800490:intron 45.0%
TGCGCGTGGGAATAAAAGAT+TGG - chr5_3:13878920-13878939 Msa0800490:intron 45.0%
TTAGTCATCAAGGTGAACCG+TGG + chr5_3:13879083-13879102 None:intergenic 45.0%
GGTTGAACCAACTGACAGAT+TGG - chr5_3:13879226-13879245 Msa0800490:intron 45.0%
CCATGGATCCTCCTAAGAAA+CGG - chr5_3:13879878-13879897 Msa0800490:CDS 45.0%
!! TTAAAAAAAAAAAATACTAA+TGG - chr5_3:13879537-13879556 Msa0800490:intron 5.0%
!! TTAAAAAAAAAAAATACTAA+TGG - chr5_3:13879537-13879556 Msa0800490:intron 5.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_3:13878043-13878062 Msa0800490:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_3:13878045-13878064 Msa0800490:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_3:13878051-13878070 None:intergenic 50.0%
GGTGTAGTGTGGAAGAAAGG+AGG - chr5_3:13878131-13878150 Msa0800490:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_3:13878286-13878305 None:intergenic 50.0%
!! AATTTTGGAAGCGGAGGAGG+AGG + chr5_3:13878315-13878334 None:intergenic 50.0%
CGAATTGGTTGAACCGGTCA+TGG - chr5_3:13879161-13879180 Msa0800490:intron 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_3:13879872-13879891 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_3:13879881-13879900 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_3:13879889-13879908 None:intergenic 50.0%
TACTTGCGTGGGGAGAACAT+GGG + chr5_3:13878041-13878060 None:intergenic 50.0%
TTACTTGCGTGGGGAGAACA+TGG + chr5_3:13878042-13878061 None:intergenic 50.0%
GTTCTCCCCACGCAAGTAAA+AGG - chr5_3:13878043-13878062 Msa0800490:three_prime_UTR 50.0%
TCTCCCCACGCAAGTAAAAG+GGG - chr5_3:13878045-13878064 Msa0800490:three_prime_UTR 50.0%
! TAGCCCCTTTTACTTGCGTG+GGG + chr5_3:13878051-13878070 None:intergenic 50.0%
GGTGTAGTGTGGAAGAAAGG+AGG - chr5_3:13878131-13878150 Msa0800490:three_prime_UTR 50.0%
!! TGGTGTTGCTGCCCTTGAAA+CGG + chr5_3:13878286-13878305 None:intergenic 50.0%
!! AATTTTGGAAGCGGAGGAGG+AGG + chr5_3:13878315-13878334 None:intergenic 50.0%
CGAATTGGTTGAACCGGTCA+TGG - chr5_3:13879161-13879180 Msa0800490:intron 50.0%
AGGAGGATCCATGGAAGCTA+AGG + chr5_3:13879872-13879891 None:intergenic 50.0%
CCGTTTCTTAGGAGGATCCA+TGG + chr5_3:13879881-13879900 None:intergenic 50.0%
GTAGAGGTCCGTTTCTTAGG+AGG + chr5_3:13879889-13879908 None:intergenic 50.0%
!! CGGAATTTTGGAAGCGGAGG+AGG + chr5_3:13878318-13878337 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_3:13878434-13878453 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_3:13878906-13878925 Msa0800490:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_3:13878907-13878926 Msa0800490:intron 55.0%
GAATCGAGCTGAGCCATGAC+CGG + chr5_3:13879177-13879196 None:intergenic 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_3:13879864-13879883 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_3:13879861-13879880 Msa0800490:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_3:13879868-13879887 None:intergenic 55.0%
!! CGGAATTTTGGAAGCGGAGG+AGG + chr5_3:13878318-13878337 None:intergenic 55.0%
! CTGCAGCCTTGATAGCAGCA+AGG + chr5_3:13878434-13878453 None:intergenic 55.0%
! TGAGGACAAAGAAGTGCGCG+TGG - chr5_3:13878906-13878925 Msa0800490:intron 55.0%
! GAGGACAAAGAAGTGCGCGT+GGG - chr5_3:13878907-13878926 Msa0800490:intron 55.0%
GAATCGAGCTGAGCCATGAC+CGG + chr5_3:13879177-13879196 None:intergenic 55.0%
CCATGGAAGCTAAGGCTGGT+TGG + chr5_3:13879864-13879883 None:intergenic 55.0%
CCAACCAGCCTTAGCTTCCA+TGG - chr5_3:13879861-13879880 Msa0800490:CDS 55.0%
GGATCCATGGAAGCTAAGGC+TGG + chr5_3:13879868-13879887 None:intergenic 55.0%
TTTGGAAGCGGAGGAGGAGG+AGG + chr5_3:13878312-13878331 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_3:13878464-13878483 None:intergenic 60.0%
TTTGGAAGCGGAGGAGGAGG+AGG + chr5_3:13878312-13878331 None:intergenic 60.0%
TTACCGTGAGGTTGCGCGCA+CGG + chr5_3:13878464-13878483 None:intergenic 60.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_3:13878458-13878477 Msa0800490:CDS 65.0%
ACTCCGTGCGCGCAACCTCA+CGG - chr5_3:13878458-13878477 Msa0800490:CDS 65.0%
AGCGGAGGAGGAGGAGGTGG+TGG + chr5_3:13878306-13878325 None:intergenic 70.0%
GGAAGCGGAGGAGGAGGAGG+TGG + chr5_3:13878309-13878328 None:intergenic 70.0%
AGCGGAGGAGGAGGAGGTGG+TGG + chr5_3:13878306-13878325 None:intergenic 70.0%
GGAAGCGGAGGAGGAGGAGG+TGG + chr5_3:13878309-13878328 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 13878041 13880280 13878041 ID=Msa0800490;Name=Msa0800490
chr5_3 mRNA 13878041 13880280 13878041 ID=Msa0800490-mRNA-1;Parent=Msa0800490;Name=Msa0800490-mRNA-1;_AED=0.12;_eAED=0.12;_QI=217|1|1|1|1|1|3|305|172
chr5_3 exon 13878041 13878490 13878041 ID=Msa0800490-mRNA-1:exon:5813;Parent=Msa0800490-mRNA-1
chr5_3 exon 13879380 13879531 13879380 ID=Msa0800490-mRNA-1:exon:5812;Parent=Msa0800490-mRNA-1
chr5_3 exon 13879842 13880280 13879842 ID=Msa0800490-mRNA-1:exon:5811;Parent=Msa0800490-mRNA-1
chr5_3 five_prime_UTR 13880064 13880280 13880064 ID=Msa0800490-mRNA-1:five_prime_utr;Parent=Msa0800490-mRNA-1
chr5_3 CDS 13879842 13880063 13879842 ID=Msa0800490-mRNA-1:cds;Parent=Msa0800490-mRNA-1
chr5_3 CDS 13879380 13879531 13879380 ID=Msa0800490-mRNA-1:cds;Parent=Msa0800490-mRNA-1
chr5_3 CDS 13878346 13878490 13878346 ID=Msa0800490-mRNA-1:cds;Parent=Msa0800490-mRNA-1
chr5_3 three_prime_UTR 13878041 13878345 13878041 ID=Msa0800490-mRNA-1:three_prime_utr;Parent=Msa0800490-mRNA-1
Gene Sequence

>Msa0800490

ATGAAGCAAAGAAAGGAACAAGCAACCGTTTCAAGGGCAGCAACACCACCACCTCCTCCTCCTCCGCTTCCAAAATTCCGAATCATCTCAAAACCAAGAACAGAAGAGAGTGTGACAAAGCGCGAAATTGCCAAGTTTTGGAGGCAAAAGCGAATTGTGGAAGAGGATCATCTCCTTGCTGCTATCAAGGCTGCAGCAAGACTCCGTGCGCGCAACCTCACGGAGCAAGAGTATCTGAGGTTTGAACTGAGCTTAAAGAATGAGGGTGATGAAGATGAGGCCAATAAAGAGACTATCAATTTGGTTGTAGGGGCAAATTATTGTGGCAATAAAGTTGATGAGGACAAAGAAGTGCGCGTGGGAATAAAAGATTGGTGGACTAAAAGCAAGTATGCATATTTGAACCAACCAGCCTTAGCTTCCATGGATCCTCCTAAGAAACGGACCTCTACTTATGTTCCACATATTCTTTCTTACAAGCATAAGGCTTTGTATCCTACTGCTATTGGTGTCTTTTGA
Protein sequence

>Msa0800490

MKQRKEQATVSRAATPPPPPPPLPKFRIISKPRTEESVTKREIAKFWRQKRIVEEDHLLAAIKAAARLRARNLTEQEYLRFELSLKNEGDEDEANKETINLVVGANYCGNKVDEDKEVRVGIKDWWTKSKYAYLNQPALASMDPPKKRTSTYVPHILSYKHKALYPTAIGVF*