AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0862350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862350 MtrunA17_Chr5g0443741 97.521 121 3 0 1 121 1 121 2.35e-81 234
Msa0862350 MtrunA17_Chr7g0257051 37.815 119 74 0 1 119 107 225 1.07e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0862350 AT1G50740.1 63.866 119 43 0 1 119 1 119 3.49e-53 163
Msa0862350 AT3G20510.1 64.706 119 42 0 1 119 1 119 9.25e-53 162
Msa0862350 AT3G57280.1 38.017 121 75 0 1 121 100 220 2.25e-17 75.1
Msa0862350 AT2G26240.1 40.196 102 55 2 6 107 9 104 6.53e-12 58.2

Find 56 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAGCGGTACTTCCTTTA+TGG 0.180195 5_4:+61963617 None:intergenic
TATGCGTCTGTCTTGCATTT+AGG 0.241572 5_4:+61960949 None:intergenic
TATGCCAGATTTCATATAAA+AGG 0.246802 5_4:+61960825 None:intergenic
ATTTGTGAGAAAAGAAAATT+TGG 0.248706 5_4:+61963734 None:intergenic
CTCGCCGGCGGCGTCGGAAC+CGG 0.266751 5_4:-61963595 Msa0862350:CDS
AATTCCGTACGGATTGTTAT+TGG 0.288264 5_4:-61963665 Msa0862350:CDS
TACTTAGTGATTTCAGAGTT+TGG 0.326976 5_4:-61961094 Msa0862350:three_prime_UTR
TTGGTCGGTGGAGGATTATT+CGG 0.355132 5_4:-61963646 Msa0862350:CDS
TGTTTAATATATAAGAGAAT+TGG 0.362436 5_4:-61961049 Msa0862350:three_prime_UTR
TATCTCAGTCTCAATGCCTT+TGG 0.362461 5_4:-61963550 Msa0862350:CDS
ATCTCAGTCTCAATGCCTTT+GGG 0.363013 5_4:-61963549 Msa0862350:CDS
AGGTCATAATACATTTCATT+TGG 0.364659 5_4:+61960893 None:intergenic
ATGCCTGCTGGTATGATTGC+AGG 0.367119 5_4:-61963019 Msa0862350:CDS
TCGCCGGCGGCGTCGGAACC+GGG 0.377853 5_4:-61963594 Msa0862350:CDS
GAATTTGAAGTGAAGTGAAT+TGG 0.386815 5_4:+61963759 None:intergenic
TTGCACAGTAGCCAAGTAAT+AGG 0.408980 5_4:+61960873 None:intergenic
TATAATACGTATATGTTAGA+TGG 0.409331 5_4:-61961133 Msa0862350:three_prime_UTR
ACATTGTCAGCATTTAGATT+AGG 0.424911 5_4:-61960798 Msa0862350:three_prime_UTR
GGTGCAATCACAGAAGGAAA+TGG 0.448983 5_4:+61960846 None:intergenic
TATCTGTACAAACTAGCAAC+TGG 0.454289 5_4:-61961216 Msa0862350:CDS
AATTTGAAGTGAAGTGAATT+GGG 0.459009 5_4:+61963760 None:intergenic
TTATTCGGTTTCATCCATAA+AGG 0.468761 5_4:-61963631 Msa0862350:CDS
GGCATTGAGACTGAGATAAC+CGG 0.470227 5_4:+61963555 None:intergenic
GTCTCAATGCCTTTGGGAAG+CGG 0.473844 5_4:-61963543 Msa0862350:CDS
GCTTCTCTCGCCGGCGGCGT+CGG 0.474606 5_4:-61963601 Msa0862350:CDS
ATGTATTATGACCTATTACT+TGG 0.475469 5_4:-61960884 Msa0862350:three_prime_UTR
GACTGCATTGTTCTGTAAGC+AGG 0.484846 5_4:-61961022 Msa0862350:three_prime_UTR
AATGTGTATACTTTACTTTG+AGG 0.488211 5_4:+61963820 None:intergenic
CAGTCAGCCTTAGCAGTAGC+AGG 0.498958 5_4:+61961180 None:intergenic
AGGTGTACTAACATGGATCA+TGG 0.511770 5_4:-61963071 Msa0862350:CDS
GGATTGTTATTGGTCGGTGG+AGG 0.525170 5_4:-61963655 Msa0862350:CDS
TTCTGTTTCACAATTCCGTA+CGG 0.532838 5_4:-61963676 Msa0862350:CDS
GGATCTCGATGGCGAGAGCA+AGG 0.538621 5_4:+61963509 None:intergenic
ACATCTAAGATTATGCCTGC+TGG 0.542306 5_4:-61963031 Msa0862350:CDS
ATACTTTACTTTGAGGCGTG+TGG 0.543748 5_4:+61963827 None:intergenic
CTGGTATGATTGCAGGACTC+AGG 0.544138 5_4:-61963012 Msa0862350:intron
TCATATACCTGCTACTGCTA+AGG 0.552394 5_4:-61961187 Msa0862350:CDS
TGTTGCAAAACAGTTTGTGC+AGG 0.563627 5_4:-61963091 Msa0862350:intron
CGACCCGGTTCCGACGCCGC+CGG 0.568570 5_4:+61963591 None:intergenic
GGCGAGGATGAGAAGCGACC+CGG 0.569011 5_4:+61963576 None:intergenic
CCGTACGGATTGTTATTGGT+CGG 0.572524 5_4:-61963661 Msa0862350:CDS
AGTCCTGCAATCATACCAGC+AGG 0.578660 5_4:+61963016 None:intergenic
GGAAGTACCGCTTCTCTCGC+CGG 0.585532 5_4:-61963610 Msa0862350:CDS
TACGGATTGTTATTGGTCGG+TGG 0.593122 5_4:-61963658 Msa0862350:CDS
GACGCCGCCGGCGAGAGAAG+CGG 0.604749 5_4:+61963603 None:intergenic
GGTGTACTAACATGGATCAT+GGG 0.605410 5_4:-61963070 Msa0862350:CDS
GGGTCGCTTCTCATCCTCGC+CGG 0.607904 5_4:-61963574 Msa0862350:CDS
ATAAAAGGTGCAATCACAGA+AGG 0.610573 5_4:+61960840 None:intergenic
TTTGTGCAGGTGTACTAACA+TGG 0.620050 5_4:-61963078 Msa0862350:CDS
CCGACCAATAACAATCCGTA+CGG 0.654910 5_4:+61963661 None:intergenic
AAAGCGCATACGGATCTCGA+TGG 0.662369 5_4:+61963498 None:intergenic
CTGTACAAACTAGCAACTGG+TGG 0.664442 5_4:-61961213 Msa0862350:CDS
TTGAGGCGTGTGGAATACAC+AGG 0.685817 5_4:+61963837 None:intergenic
GAAGTGAATTGGGACAACGA+TGG 0.708088 5_4:+61963770 None:intergenic
AGTACCGCTTCTCTCGCCGG+CGG 0.718006 5_4:-61963607 Msa0862350:CDS
TGAGACTGAGATAACCGGCG+AGG 0.730012 5_4:+61963560 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAATAAAATATGCTAT+TGG + chr5_4:61962299-61962318 None:intergenic 10.0%
!! TTTAATGCATATTACTTATA+AGG + chr5_4:61961296-61961315 None:intergenic 15.0%
!! AATAGTATTATTTACATGTT+TGG + chr5_4:61961505-61961524 None:intergenic 15.0%
!! TTAATACAAGATTTAGTTAT+TGG - chr5_4:61961686-61961705 Msa0862350:intron 15.0%
!! TTCTGTAACAAAAAAAAAAA+AGG - chr5_4:61961710-61961729 Msa0862350:intron 15.0%
!! TCTGTAACAAAAAAAAAAAA+GGG - chr5_4:61961711-61961730 Msa0862350:intron 15.0%
!! TAGATAGAATTTATAAAGTT+TGG - chr5_4:61961964-61961983 Msa0862350:intron 15.0%
!! AGATAGAATTTATAAAGTTT+GGG - chr5_4:61961965-61961984 Msa0862350:intron 15.0%
!!! AAAAGATTGAAATGAATTTT+TGG - chr5_4:61962697-61962716 Msa0862350:intron 15.0%
!! AACTTTATAAACTTTGATTA+GGG + chr5_4:61963008-61963027 None:intergenic 15.0%
!! AAACTTTATAAACTTTGATT+AGG + chr5_4:61963009-61963028 None:intergenic 15.0%
!!! AATCAAAGTTTATAAAGTTT+TGG - chr5_4:61963009-61963028 Msa0862350:intron 15.0%
!!! TTGATTTCTGAAATAATATT+TGG + chr5_4:61963150-61963169 None:intergenic 15.0%
!! TGTTTAATATATAAGAGAAT+TGG - chr5_4:61963572-61963591 Msa0862350:CDS 15.0%
!! ATTTGTGAGAAAAGAAAATT+TGG + chr5_4:61960890-61960909 None:intergenic 20.0%
!!! TTGAGTTTAAATGTCAATTT+TGG - chr5_4:61961451-61961470 Msa0862350:intron 20.0%
!!! AAGTACTTAGTTTTATTTGT+AGG - chr5_4:61961650-61961669 Msa0862350:intron 20.0%
!! AAAAAAAAGGGATTTATTGT+TGG - chr5_4:61961723-61961742 Msa0862350:intron 20.0%
!! AATGCATTAACTATTCATAA+AGG + chr5_4:61961789-61961808 None:intergenic 20.0%
!!! TCTTTTATTCTGAAAGTAAA+TGG + chr5_4:61961874-61961893 None:intergenic 20.0%
!! TAATAAAATATGCTATTGGT+CGG + chr5_4:61962295-61962314 None:intergenic 20.0%
!! GAAGATGAATATAGAAATAA+AGG + chr5_4:61962511-61962530 None:intergenic 20.0%
!! TTGAGATGAAAAATAATAGA+AGG + chr5_4:61962557-61962576 None:intergenic 20.0%
!!! TGAATCTGAAATTTTGTTTA+TGG - chr5_4:61962660-61962679 Msa0862350:intron 20.0%
!! TATAATACGTATATGTTAGA+TGG - chr5_4:61963488-61963507 Msa0862350:intron 20.0%
! AATGTGTATACTTTACTTTG+AGG + chr5_4:61960804-61960823 None:intergenic 25.0%
!! AATTTGAAGTGAAGTGAATT+GGG + chr5_4:61960864-61960883 None:intergenic 25.0%
!!! TGATTTTGATTCGTATTTTG+TGG - chr5_4:61961227-61961246 Msa0862350:CDS 25.0%
! TGCACATTAAATAAAAATGC+AGG + chr5_4:61961340-61961359 None:intergenic 25.0%
!!! TCAAGTGATCTATTTTTGTT+TGG - chr5_4:61961394-61961413 Msa0862350:intron 25.0%
!! AGTTTAAATGTCAATTTTGG+AGG - chr5_4:61961454-61961473 Msa0862350:intron 25.0%
! CATAATCATTTCTATTCTAG+AGG - chr5_4:61961477-61961496 Msa0862350:intron 25.0%
! ATGAATAGTTAATGCATTCA+CGG - chr5_4:61961791-61961810 Msa0862350:intron 25.0%
!!! ACTATTTTTCTAGCTAATGA+AGG - chr5_4:61961836-61961855 Msa0862350:intron 25.0%
! TTCTATATTCATCTTCTCTA+AGG - chr5_4:61962515-61962534 Msa0862350:intron 25.0%
!!! TGAAGTATTTCTTTTTCTTC+TGG - chr5_4:61962751-61962770 Msa0862350:intron 25.0%
!!! GAAGTATTTCTTTTTCTTCT+GGG - chr5_4:61962752-61962771 Msa0862350:intron 25.0%
! TCAAAGATTCATGATTAGAA+AGG + chr5_4:61962783-61962802 None:intergenic 25.0%
! TTGTAAACATTATGCAAGTT+TGG - chr5_4:61963244-61963263 Msa0862350:intron 25.0%
! AGGTCATAATACATTTCATT+TGG + chr5_4:61963731-61963750 None:intergenic 25.0%
! ATGTATTATGACCTATTACT+TGG - chr5_4:61963737-61963756 Msa0862350:five_prime_UTR 25.0%
! TATGCCAGATTTCATATAAA+AGG + chr5_4:61963799-61963818 None:intergenic 25.0%
! GAATTTGAAGTGAAGTGAAT+TGG + chr5_4:61960865-61960884 None:intergenic 30.0%
TTATTCGGTTTCATCCATAA+AGG - chr5_4:61960990-61961009 Msa0862350:three_prime_UTR 30.0%
! TGAAATGAAAAAAGCGCATA+CGG + chr5_4:61961136-61961155 None:intergenic 30.0%
ACTTATTCTAATTGTGCTGT+TGG - chr5_4:61962063-61962082 Msa0862350:intron 30.0%
! CTTATTCTAATTGTGCTGTT+GGG - chr5_4:61962064-61962083 Msa0862350:intron 30.0%
TGTGATGAAGATGCAAAATT+GGG + chr5_4:61962415-61962434 None:intergenic 30.0%
AAAGAACTTGCACTAAACAA+GGG + chr5_4:61963076-61963095 None:intergenic 30.0%
AAAAGAACTTGCACTAAACA+AGG + chr5_4:61963077-61963096 None:intergenic 30.0%
!! TGTTTGTTTGCTAGTCTTAA+TGG - chr5_4:61963194-61963213 Msa0862350:intron 30.0%
TACTTAGTGATTTCAGAGTT+TGG - chr5_4:61963527-61963546 Msa0862350:CDS 30.0%
TTCATGAAGAAAAAGAGGAA+AGG + chr5_4:61963639-61963658 None:intergenic 30.0%
AATGCTTCATGAAGAAAAAG+AGG + chr5_4:61963644-61963663 None:intergenic 30.0%
! TCTTTTTCTTCATGAAGCAT+TGG - chr5_4:61963643-61963662 Msa0862350:CDS 30.0%
!! CTTTTTCTTCATGAAGCATT+GGG - chr5_4:61963644-61963663 Msa0862350:CDS 30.0%
!!! TTTTTCTTCATGAAGCATTG+GGG - chr5_4:61963645-61963664 Msa0862350:CDS 30.0%
! TGCACCTTTTATATGAAATC+TGG - chr5_4:61963792-61963811 Msa0862350:five_prime_UTR 30.0%
ACATTGTCAGCATTTAGATT+AGG - chr5_4:61963823-61963842 Msa0862350:five_prime_UTR 30.0%
TTCTGTTTCACAATTCCGTA+CGG - chr5_4:61960945-61960964 Msa0862350:three_prime_UTR 35.0%
! AATTCCGTACGGATTGTTAT+TGG - chr5_4:61960956-61960975 Msa0862350:three_prime_UTR 35.0%
! TGCATTCACGGATGTATTTT+TGG - chr5_4:61961803-61961822 Msa0862350:intron 35.0%
! GACTTGAGTTTTTCTCCTAA+GGG + chr5_4:61961921-61961940 None:intergenic 35.0%
! AGACTTGAGTTTTTCTCCTA+AGG + chr5_4:61961922-61961941 None:intergenic 35.0%
GAAAAACTCAAGTCTCAAGT+TGG - chr5_4:61961927-61961946 Msa0862350:intron 35.0%
!!! ACGCCTTACAAGTTGATTTT+AGG - chr5_4:61961996-61962015 Msa0862350:intron 35.0%
!!! CGCCTTACAAGTTGATTTTA+GGG - chr5_4:61961997-61962016 Msa0862350:intron 35.0%
!!! GCCTTACAAGTTGATTTTAG+GGG - chr5_4:61961998-61962017 Msa0862350:intron 35.0%
ACCCCTAAAATCAACTTGTA+AGG + chr5_4:61962002-61962021 None:intergenic 35.0%
AAAATATGCTATTGGTCGGA+CGG + chr5_4:61962291-61962310 None:intergenic 35.0%
! AAACAATAACAGCGACGTTT+TGG - chr5_4:61962350-61962369 Msa0862350:intron 35.0%
GTGTGATGAAGATGCAAAAT+TGG + chr5_4:61962416-61962435 None:intergenic 35.0%
AATAAAGGCAGAAATCACAG+TGG + chr5_4:61962496-61962515 None:intergenic 35.0%
TTGTGTCGAATTGTAAAGTG+AGG + chr5_4:61963039-61963058 None:intergenic 35.0%
CAATGCACATTCATAAGTAG+CGG + chr5_4:61963291-61963310 None:intergenic 35.0%
!! GCAATGCATGTTTTTGTATC+TGG - chr5_4:61963330-61963349 Msa0862350:intron 35.0%
! TTCCTTTTCTTTTTGTGCAG+TGG - chr5_4:61963366-61963385 Msa0862350:intron 35.0%
TATCTGTACAAACTAGCAAC+TGG - chr5_4:61963405-61963424 Msa0862350:intron 35.0%
! ATAAAAGGTGCAATCACAGA+AGG + chr5_4:61963784-61963803 None:intergenic 35.0%
!! ATACTTTACTTTGAGGCGTG+TGG + chr5_4:61960797-61960816 None:intergenic 40.0%
CAACGATGGAGAAAAAGTCT+TGG + chr5_4:61960840-61960859 None:intergenic 40.0%
TATCTCAGTCTCAATGCCTT+TGG - chr5_4:61961071-61961090 Msa0862350:three_prime_UTR 40.0%
ATCTCAGTCTCAATGCCTTT+GGG - chr5_4:61961072-61961091 Msa0862350:three_prime_UTR 40.0%
TGTTGCAAAACAGTTTGTGC+AGG - chr5_4:61961530-61961549 Msa0862350:intron 40.0%
TTTGTGCAGGTGTACTAACA+TGG - chr5_4:61961543-61961562 Msa0862350:intron 40.0%
AGGTGTACTAACATGGATCA+TGG - chr5_4:61961550-61961569 Msa0862350:intron 40.0%
GGTGTACTAACATGGATCAT+GGG - chr5_4:61961551-61961570 Msa0862350:intron 40.0%
! ACATCTAAGATTATGCCTGC+TGG - chr5_4:61961590-61961609 Msa0862350:intron 40.0%
GCAGGACTCAGGTAATTAAA+AGG - chr5_4:61961620-61961639 Msa0862350:intron 40.0%
AGTTTGCGATATCATCCCTT+AGG - chr5_4:61961903-61961922 Msa0862350:intron 40.0%
!! ACTCAAGTCTCAAGTTGGTT+AGG - chr5_4:61961932-61961951 Msa0862350:intron 40.0%
!! CTCAAGTCTCAAGTTGGTTA+GGG - chr5_4:61961933-61961952 Msa0862350:intron 40.0%
CATACATGCATAGATCTGTC+CGG - chr5_4:61962580-61962599 Msa0862350:intron 40.0%
CAAGATGAAAGAAGCCATTC+CGG + chr5_4:61962602-61962621 None:intergenic 40.0%
CTATTGTGTTGTGTGGAGTA+GGG + chr5_4:61962985-61963004 None:intergenic 40.0%
ATTAGGGCTATTGTGTTGTG+TGG + chr5_4:61962992-61963011 None:intergenic 40.0%
GACCACTGCACAAAAAGAAA+AGG + chr5_4:61963371-61963390 None:intergenic 40.0%
TCATATACCTGCTACTGCTA+AGG - chr5_4:61963434-61963453 Msa0862350:intron 40.0%
TATGCGTCTGTCTTGCATTT+AGG + chr5_4:61963675-61963694 None:intergenic 40.0%
TTGCACAGTAGCCAAGTAAT+AGG + chr5_4:61963751-61963770 None:intergenic 40.0%
GAAGTGAATTGGGACAACGA+TGG + chr5_4:61960854-61960873 None:intergenic 45.0%
CCGACCAATAACAATCCGTA+CGG + chr5_4:61960963-61960982 None:intergenic 45.0%
! CCGTACGGATTGTTATTGGT+CGG - chr5_4:61960960-61960979 Msa0862350:three_prime_UTR 45.0%
! TACGGATTGTTATTGGTCGG+TGG - chr5_4:61960963-61960982 Msa0862350:three_prime_UTR 45.0%
TTGGTCGGTGGAGGATTATT+CGG - chr5_4:61960975-61960994 Msa0862350:three_prime_UTR 45.0%
GAGAAGCGGTACTTCCTTTA+TGG + chr5_4:61961007-61961026 None:intergenic 45.0%
! GGCATTGAGACTGAGATAAC+CGG + chr5_4:61961069-61961088 None:intergenic 45.0%
! AGAGTTTTTCCGCTTCCCAA+AGG + chr5_4:61961090-61961109 None:intergenic 45.0%
TATCGCTGGTCCATGAATCT+TGG + chr5_4:61962261-61962280 None:intergenic 45.0%
!! ATAACAGCGACGTTTTGGTG+TGG - chr5_4:61962355-61962374 Msa0862350:intron 45.0%
!! TAACAGCGACGTTTTGGTGT+GGG - chr5_4:61962356-61962375 Msa0862350:intron 45.0%
AAATCACAGTGGTGGTTCGT+TGG + chr5_4:61962485-61962504 None:intergenic 45.0%
AAAGGCAGAAATCACAGTGG+TGG + chr5_4:61962493-61962512 None:intergenic 45.0%
ATGCATAGATCTGTCCGGAA+TGG - chr5_4:61962585-61962604 Msa0862350:intron 45.0%
GCTATTGTGTTGTGTGGAGT+AGG + chr5_4:61962986-61963005 None:intergenic 45.0%
AACTTGCACTAAACAAGGGC+AGG + chr5_4:61963072-61963091 None:intergenic 45.0%
CTGTACAAACTAGCAACTGG+TGG - chr5_4:61963408-61963427 Msa0862350:intron 45.0%
GACTGCATTGTTCTGTAAGC+AGG - chr5_4:61963599-61963618 Msa0862350:CDS 45.0%
GGTGCAATCACAGAAGGAAA+TGG + chr5_4:61963778-61963797 None:intergenic 45.0%
! GGATTGTTATTGGTCGGTGG+AGG - chr5_4:61960966-61960985 Msa0862350:three_prime_UTR 50.0%
! GTCTCAATGCCTTTGGGAAG+CGG - chr5_4:61961078-61961097 Msa0862350:three_prime_UTR 50.0%
AAAGCGCATACGGATCTCGA+TGG + chr5_4:61961126-61961145 None:intergenic 50.0%
ATGCCTGCTGGTATGATTGC+AGG - chr5_4:61961602-61961621 Msa0862350:intron 50.0%
AGTCCTGCAATCATACCAGC+AGG + chr5_4:61961608-61961627 None:intergenic 50.0%
CTGGTATGATTGCAGGACTC+AGG - chr5_4:61961609-61961628 Msa0862350:intron 50.0%
TTGTCTGCACACTCCACACT+AGG - chr5_4:61962096-61962115 Msa0862350:intron 50.0%
TGTCTGCACACTCCACACTA+GGG - chr5_4:61962097-61962116 Msa0862350:intron 50.0%
! TTTTCTGTCCAGCCCTAGTG+TGG + chr5_4:61962112-61962131 None:intergenic 50.0%
CACGTCTATCCACATGCAAC+CGG + chr5_4:61962226-61962245 None:intergenic 50.0%
GGTTGCATGTGGATAGACGT+GGG - chr5_4:61962225-61962244 Msa0862350:intron 50.0%
!! TCGGTTCGGACCAAGATTCA+TGG - chr5_4:61962248-61962267 Msa0862350:intron 50.0%
GTTCGTTGGAGTGAGAAGGT+AGG + chr5_4:61962471-61962490 None:intergenic 50.0%
! TGTCGGTTGATGCGGTTTGT+CGG + chr5_4:61962848-61962867 None:intergenic 50.0%
! TTGTCGGATGTCGGTTGATG+CGG + chr5_4:61962856-61962875 None:intergenic 50.0%
!!! TCGTCGGTTTTGTCGGATGT+CGG + chr5_4:61962865-61962884 None:intergenic 50.0%
!!! GTCTGTGTCGTCGGTTTTGT+CGG + chr5_4:61962872-61962891 None:intergenic 50.0%
GACAAAACCGACGACACAGA+CGG - chr5_4:61962871-61962890 Msa0862350:intron 50.0%
ATTTCGTCCGTCTGTGTCGT+CGG + chr5_4:61962881-61962900 None:intergenic 50.0%
ACGACACAGACGGACGAAAT+TGG - chr5_4:61962881-61962900 Msa0862350:intron 50.0%
CGACACAGACGGACGAAATT+GGG - chr5_4:61962882-61962901 Msa0862350:intron 50.0%
ACAGACGGACGAAATTGGGT+GGG - chr5_4:61962886-61962905 Msa0862350:intron 50.0%
!! TTCTCACCCGTGGTTTGCTT+CGG - chr5_4:61962912-61962931 Msa0862350:intron 50.0%
TGAGACTGAGATAACCGGCG+AGG + chr5_4:61961064-61961083 None:intergenic 55.0%
GCTATCCGACCCAAACTGTC+CGG - chr5_4:61962134-61962153 Msa0862350:intron 55.0%
CTGTTCGGTCCGGACAGTTT+GGG + chr5_4:61962146-61962165 None:intergenic 55.0%
CTATCCACATGCAACCGGCT+TGG + chr5_4:61962221-61962240 None:intergenic 55.0%
CGGTTGCATGTGGATAGACG+TGG - chr5_4:61962224-61962243 Msa0862350:intron 55.0%
GCATGTGGATAGACGTGGGT+CGG - chr5_4:61962229-61962248 Msa0862350:intron 55.0%
TGGATAGACGTGGGTCGGTT+CGG - chr5_4:61962234-61962253 Msa0862350:intron 55.0%
GCTATTGGTCGGACGGTAGT+GGG + chr5_4:61962284-61962303 None:intergenic 55.0%
TGCTATTGGTCGGACGGTAG+TGG + chr5_4:61962285-61962304 None:intergenic 55.0%
GGTGGTTCGTTGGAGTGAGA+AGG + chr5_4:61962475-61962494 None:intergenic 55.0%
ATGCGGTTTGTCGGATGTGC+GGG + chr5_4:61962839-61962858 None:intergenic 55.0%
GATGCGGTTTGTCGGATGTG+CGG + chr5_4:61962840-61962859 None:intergenic 55.0%
CACAGACGGACGAAATTGGG+TGG - chr5_4:61962885-61962904 Msa0862350:intron 55.0%
!! ACCCGTGGTTTGCTTCGGAT+TGG - chr5_4:61962917-61962936 Msa0862350:intron 55.0%
CCCAATCCGAAGCAAACCAC+GGG + chr5_4:61962921-61962940 None:intergenic 55.0%
!! CCCGTGGTTTGCTTCGGATT+GGG - chr5_4:61962918-61962937 Msa0862350:intron 55.0%
CCCCAATCCGAAGCAAACCA+CGG + chr5_4:61962922-61962941 None:intergenic 55.0%
!! CCGTGGTTTGCTTCGGATTG+GGG - chr5_4:61962919-61962938 Msa0862350:intron 55.0%
TGTGGAGTAGGGCTGGACAT+CGG + chr5_4:61962974-61962993 None:intergenic 55.0%
TGTGTTGTGTGGAGTAGGGC+TGG + chr5_4:61962981-61963000 None:intergenic 55.0%
CAGTCAGCCTTAGCAGTAGC+AGG + chr5_4:61963444-61963463 None:intergenic 55.0%
!! GGAAGTACCGCTTCTCTCGC+CGG - chr5_4:61961011-61961030 Msa0862350:three_prime_UTR 60.0%
GGATCTCGATGGCGAGAGCA+AGG + chr5_4:61961115-61961134 None:intergenic 60.0%
TGCACACTCCACACTAGGGC+TGG - chr5_4:61962101-61962120 Msa0862350:intron 60.0%
TCGGTCCGGACAGTTTGGGT+CGG + chr5_4:61962142-61962161 None:intergenic 60.0%
GCTGTTCGGTCCGGACAGTT+TGG + chr5_4:61962147-61962166 None:intergenic 60.0%
! AACGGGTGGAGCGGGTTTCA+CGG - chr5_4:61962190-61962209 Msa0862350:intron 60.0%
GTTTCACGGGCAAGCCAAGC+CGG - chr5_4:61962204-61962223 Msa0862350:intron 60.0%
CAAGCCAAGCCGGTTGCATG+TGG - chr5_4:61962214-61962233 Msa0862350:intron 60.0%
! GATGTGCGGGTGGTTTGTGG+CGG + chr5_4:61962826-61962845 None:intergenic 60.0%
TCGGATGTGCGGGTGGTTTG+TGG + chr5_4:61962829-61962848 None:intergenic 60.0%
GGGTGGGATATTCTCACCCG+TGG - chr5_4:61962902-61962921 Msa0862350:intron 60.0%
GGTCGGTTGCGGTCGGATTT+CGG + chr5_4:61962953-61962972 None:intergenic 60.0%
GAGTAGGGCTGGACATCGGT+CGG + chr5_4:61962970-61962989 None:intergenic 60.0%
AGTACCGCTTCTCTCGCCGG+CGG - chr5_4:61961014-61961033 Msa0862350:three_prime_UTR 65.0%
GGCGAGGATGAGAAGCGACC+CGG + chr5_4:61961048-61961067 None:intergenic 65.0%
GGGTCGCTTCTCATCCTCGC+CGG - chr5_4:61961047-61961066 Msa0862350:three_prime_UTR 65.0%
GTCGGCTGAAACGGGTGGAG+CGG - chr5_4:61962181-61962200 Msa0862350:intron 65.0%
TCGGCTGAAACGGGTGGAGC+GGG - chr5_4:61962182-61962201 Msa0862350:intron 65.0%
! ACGGGTGGAGCGGGTTTCAC+GGG - chr5_4:61962191-61962210 Msa0862350:intron 65.0%
!! CGGACGGTAGTGGGTATCGC+TGG + chr5_4:61962275-61962294 None:intergenic 65.0%
CGGTTTGTCGGATGTGCGGG+TGG + chr5_4:61962836-61962855 None:intergenic 65.0%
GGACATCGGTCGGTTGCGGT+CGG + chr5_4:61962960-61962979 None:intergenic 65.0%
GGCTGGACATCGGTCGGTTG+CGG + chr5_4:61962964-61962983 None:intergenic 65.0%
!! CGCGAACGGGCTGTTCGGTC+CGG + chr5_4:61962156-61962175 None:intergenic 70.0%
!! CCGTGCGCGAACGGGCTGTT+CGG + chr5_4:61962161-61962180 None:intergenic 70.0%
CCGAACAGCCCGTTCGCGCA+CGG - chr5_4:61962158-61962177 Msa0862350:intron 70.0%
CGAACAGCCCGTTCGCGCAC+GGG - chr5_4:61962159-61962178 Msa0862350:intron 70.0%
TCAGCCGACCCGTGCGCGAA+CGG + chr5_4:61962170-61962189 None:intergenic 70.0%
CGCGCACGGGTCGGCTGAAA+CGG - chr5_4:61962172-61962191 Msa0862350:intron 70.0%
GCGCACGGGTCGGCTGAAAC+GGG - chr5_4:61962173-61962192 Msa0862350:intron 70.0%
CACGGGTCGGCTGAAACGGG+TGG - chr5_4:61962176-61962195 Msa0862350:intron 70.0%
GACGCCGCCGGCGAGAGAAG+CGG + chr5_4:61961021-61961040 None:intergenic 75.0%
GCTTCTCTCGCCGGCGGCGT+CGG - chr5_4:61961020-61961039 Msa0862350:three_prime_UTR 75.0%
CAGCCCGTTCGCGCACGGGT+CGG - chr5_4:61962163-61962182 Msa0862350:intron 75.0%
CAGCCGACCCGTGCGCGAAC+GGG + chr5_4:61962169-61962188 None:intergenic 75.0%
!! CTCGCCGGCGGCGTCGGAAC+CGG - chr5_4:61961026-61961045 Msa0862350:three_prime_UTR 80.0%
!! TCGCCGGCGGCGTCGGAACC+GGG - chr5_4:61961027-61961046 Msa0862350:three_prime_UTR 80.0%
! CGACCCGGTTCCGACGCCGC+CGG + chr5_4:61961033-61961052 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 61960787 61963856 61960787 ID=Msa0862350;Name=Msa0862350
chr5_4 mRNA 61960787 61963856 61960787 ID=Msa0862350-mRNA-1;Parent=Msa0862350;Name=Msa0862350-mRNA-1;_AED=0.00;_eAED=0.00;_QI=149|1|1|1|1|1|3|392|121
chr5_4 exon 61960787 61961257 61960787 ID=Msa0862350-mRNA-1:exon:19263;Parent=Msa0862350-mRNA-1
chr5_4 exon 61963013 61963100 61963013 ID=Msa0862350-mRNA-1:exon:19262;Parent=Msa0862350-mRNA-1
chr5_4 exon 61963509 61963856 61963509 ID=Msa0862350-mRNA-1:exon:19261;Parent=Msa0862350-mRNA-1
chr5_4 five_prime_UTR 61963708 61963856 61963708 ID=Msa0862350-mRNA-1:five_prime_utr;Parent=Msa0862350-mRNA-1
chr5_4 CDS 61963509 61963707 61963509 ID=Msa0862350-mRNA-1:cds;Parent=Msa0862350-mRNA-1
chr5_4 CDS 61963013 61963100 61963013 ID=Msa0862350-mRNA-1:cds;Parent=Msa0862350-mRNA-1
chr5_4 CDS 61961179 61961257 61961179 ID=Msa0862350-mRNA-1:cds;Parent=Msa0862350-mRNA-1
chr5_4 three_prime_UTR 61960787 61961178 61960787 ID=Msa0862350-mRNA-1:three_prime_utr;Parent=Msa0862350-mRNA-1
Gene Sequence

>Msa0862350

ATGCACGATTTCTGTTTCACAATTCCGTACGGATTGTTATTGGTCGGTGGAGGATTATTCGGTTTCATCCATAAAGGAAGTACCGCTTCTCTCGCCGGCGGCGTCGGAACCGGGTCGCTTCTCATCCTCGCCGGTTATCTCAGTCTCAATGCCTTTGGGAAGCGGAAAAACTCTTACCTTGCTCTCGCCATCGAGATCCTTTGTGCAGGTGTACTAACATGGATCATGGGACAACGTTACTTGCAAACATCTAAGATTATGCCTGCTGGTATGATTGCAGGACTCAGTGGTCTCATGACTCTGTTTTATCTGTACAAACTAGCAACTGGTGGCAATCATATACCTGCTACTGCTAAGGCTGACTGA
Protein sequence

>Msa0862350

MHDFCFTIPYGLLLVGGGLFGFIHKGSTASLAGGVGTGSLLILAGYLSLNAFGKRKNSYLALAIEILCAGVLTWIMGQRYLQTSKIMPAGMIAGLSGLMTLFYLYKLATGGNHIPATAKAD*