AlfalfaGEDB | Msa0823370

M. sativa cultivar zhongmu-4 / Msa0823370

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF03647.16

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0823370	sp\|Q9C6T7\|FAX5_ARATH	63.866	119	43	0	1	119	1	119	3.43e-52	163

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0823370	B7FH11	97.521	121	3	0	1	121	1	121	1.22e-77	234

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0021610	Msa0823370	0.802361	5.819534e-49	-8.615850e-47
Msa0030160	Msa0823370	0.816087	6.626841e-52	-8.615850e-47
Msa0030700	Msa0823370	0.808914	2.450546e-50	-8.615850e-47
Msa0035020	Msa0823370	0.842847	2.013439e-58	-8.615850e-47
Msa0077700	Msa0823370	0.804363	2.238919e-49	-8.615850e-47
Msa0078120	Msa0823370	0.802896	4.512558e-49	-8.615850e-47
Msa0090150	Msa0823370	0.800715	1.265692e-48	-8.615850e-47
Msa0613110	Msa0823370	0.802535	5.358238e-49	-8.615850e-47
Msa0613340	Msa0823370	0.814583	1.431106e-51	-8.615850e-47
Msa0614920	Msa0823370	0.800475	1.416279e-48	-8.615850e-47
Msa0653670	Msa0823370	0.815388	9.486591e-52	-8.615850e-47
Msa0653880	Msa0823370	0.815252	1.017070e-51	-8.615850e-47
Msa0708310	Msa0823370	0.800813	1.208408e-48	-8.615850e-47
Msa0711150	Msa0823370	0.833905	4.077386e-56	-8.615850e-47
Msa0733010	Msa0823370	0.842769	2.111645e-58	-8.615850e-47
Msa0742210	Msa0823370	0.930137	2.537307e-93	-8.615850e-47
Msa0743930	Msa0823370	0.826322	2.891090e-54	-8.615850e-47
Msa0773990	Msa0823370	0.818673	1.733366e-52	-8.615850e-47
Msa0802240	Msa0823370	0.809062	2.278413e-50	-8.615850e-47
Msa0816130	Msa0823370	0.817537	3.131807e-52	-8.615850e-47
Msa0822500	Msa0823370	0.819634	1.047527e-52	-8.615850e-47
Msa0823370	Msa0833530	0.818097	2.341859e-52	-8.615850e-47
Msa0823370	Msa0846860	0.812932	3.304567e-51	-8.615850e-47
Msa0823370	Msa0862350	0.810457	1.141948e-50	-8.615850e-47
Msa0823370	Msa0867010	0.812155	4.888801e-51	-8.615850e-47
Msa0823370	Msa0965290	0.803845	2.870507e-49	-8.615850e-47
Msa0823370	Msa0999680	0.803150	3.998549e-49	-8.615850e-47
Msa0823370	Msa1048220	0.828990	6.612460e-55	-8.615850e-47
Msa0823370	Msa1089450	0.829956	3.851426e-55	-8.615850e-47
Msa0823370	Msa1089460	0.802166	6.382927e-49	-8.615850e-47
Msa0823370	Msa1104560	0.806093	9.725490e-50	-8.615850e-47
Msa0823370	Msa1155170	-0.805593	1.238754e-49	-8.615850e-47
Msa0823370	Msa1160740	-0.807614	4.637664e-50	-8.615850e-47
Msa0823370	Msa1181140	0.805550	1.264648e-49	-8.615850e-47
Msa0823370	Msa1211230	-0.816250	6.091471e-52	-8.615850e-47
Msa0823370	Msa1225860	-0.804209	2.411406e-49	-8.615850e-47
Msa0823370	Msa1238600	0.811552	6.611187e-51	-8.615850e-47
Msa0823370	Msa1281660	0.815583	8.582289e-52	-8.615850e-47
Msa0823370	Msa1281690	0.821872	3.203861e-53	-8.615850e-47
Msa0823370	Msa1299400	-0.804093	2.549073e-49	-8.615850e-47
Msa0823370	Msa1325410	-0.800225	1.592587e-48	-8.615850e-47
Msa0823370	Msa1331050	-0.821779	3.366996e-53	-8.615850e-47
Msa0823370	Msa1345940	0.809823	1.564162e-50	-8.615850e-47
Msa0823370	Msa1353890	0.801764	7.717957e-49	-8.615850e-47
Msa0823370	Msa1355890	0.801632	8.218017e-49	-8.615850e-47
Msa0823370	Msa1380220	0.840301	9.445205e-58	-8.615850e-47
Msa0823370	Msa1390290	0.837143	6.184605e-57	-8.615850e-47
Msa0823370	Msa1450440	0.833219	6.048902e-56	-8.615850e-47
Msa0823370	Msa1452710	0.828176	1.039761e-54	-8.615850e-47
Msa0823370	Msa1460370	0.801681	8.028603e-49	-8.615850e-47
Msa0137800	Msa0823370	0.830972	2.173464e-55	-8.615850e-47
Msa0168310	Msa0823370	0.800748	1.246231e-48	-8.615850e-47
Msa0199940	Msa0823370	0.837165	6.105936e-57	-8.615850e-47
Msa0217700	Msa0823370	0.843714	1.182105e-58	-8.615850e-47
Msa0369920	Msa0823370	0.809706	1.657166e-50	-8.615850e-47
Msa0240900	Msa0823370	0.820783	5.714944e-53	-8.615850e-47
Msa0257520	Msa0823370	0.808794	2.599967e-50	-8.615850e-47
Msa0267790	Msa0823370	0.829413	5.219986e-55	-8.615850e-47
Msa0278580	Msa0823370	0.801064	1.074040e-48	-8.615850e-47
Msa0296820	Msa0823370	0.815308	9.879924e-52	-8.615850e-47
Msa0317970	Msa0823370	0.832811	7.639489e-56	-8.615850e-47
Msa0332190	Msa0823370	0.811346	7.331413e-51	-8.615850e-47
Msa0494190	Msa0823370	0.821735	3.446987e-53	-8.615850e-47
Msa0521670	Msa0823370	0.800678	1.287454e-48	-8.615850e-47
Msa0526680	Msa0823370	0.823714	1.193873e-53	-8.615850e-47
Msa0526730	Msa0823370	0.834807	2.420598e-56	-8.615850e-47
Msa0534150	Msa0823370	0.851022	1.162983e-60	-8.615850e-47
Msa0583360	Msa0823370	-0.804566	2.031124e-49	-8.615850e-47
Msa0590150	Msa0823370	0.824805	6.615253e-54	-8.615850e-47
Msa0592330	Msa0823370	0.816505	5.340971e-52	-8.615850e-47
Msa0600590	Msa0823370	0.806769	7.001815e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0823370	MtrunA17_Chr5g0443741	97.521	121	3	0	1	121	1	121	2.35e-81	234
Msa0823370	MtrunA17_Chr7g0257051	37.815	119	74	0	1	119	107	225	1.07e-17	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0823370	AT1G50740.1	63.866	119	43	0	1	119	1	119	3.49e-53	163
Msa0823370	AT3G20510.1	64.706	119	42	0	1	119	1	119	9.25e-53	162
Msa0823370	AT3G57280.1	38.017	121	75	0	1	121	100	220	2.25e-17	75.1
Msa0823370	AT2G26240.1	40.196	102	55	2	6	107	9	104	6.53e-12	58.2

Find 57 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAAGCGGTACTTCCTTTA+TGG	0.180195	5_3:-62036721	None:intergenic
TATGCGTCTGTCTTGCATTT+AGG	0.241572	5_3:-62038484	None:intergenic
TATGCCAGATTTCATATAAA+AGG	0.246802	5_3:-62038608	None:intergenic
ATTTGTGAGAAAAGAAAATT+TGG	0.248706	5_3:-62036602	None:intergenic
CTCGCCGGCGGCGTCGGAAC+CGG	0.266751	5_3:+62036743	Msa0823370:CDS
AATTCCGTACGGATTGTTAT+TGG	0.288264	5_3:+62036673	Msa0823370:CDS
TACTTGGTGATTTCAGAGTT+TGG	0.354666	5_3:+62038339	Msa0823370:three_prime_UTR
TTGGTCGGTGGAGGATTATT+CGG	0.355132	5_3:+62036692	Msa0823370:CDS
TGTTTAATATATAAGAGAAT+TGG	0.362436	5_3:+62038384	Msa0823370:three_prime_UTR
TATCTCAGTCTCAATGCCTT+TGG	0.362461	5_3:+62036788	Msa0823370:CDS
ATCTCAGTCTCAATGCCTTT+GGG	0.363013	5_3:+62036789	Msa0823370:CDS
AGGTCATAATACATTTCATT+TGG	0.364659	5_3:-62038540	None:intergenic
ATGCCTGCTGGTATGATTGC+AGG	0.367119	5_3:+62037296	Msa0823370:CDS
TCGCCGGCGGCGTCGGAACC+GGG	0.377853	5_3:+62036744	Msa0823370:CDS
GAATTTGAAGTGAAGTGAAT+TGG	0.386815	5_3:-62036577	None:intergenic
CAGAGAATGTGTATACTTTG+AGG	0.397188	5_3:-62036521	None:intergenic
TTGCACAGTAGCCAAGTAAT+AGG	0.408980	5_3:-62038560	None:intergenic
TATAATACGTATATGTTAGA+TGG	0.409331	5_3:+62038300	Msa0823370:three_prime_UTR
ACATTGTCAGCATTTAGATT+AGG	0.424911	5_3:+62038635	Msa0823370:three_prime_UTR
GGTGCAATCACAGAAGGAAA+TGG	0.448983	5_3:-62038587	None:intergenic
TATCTGTACAAACTAGCAAC+TGG	0.454289	5_3:+62038217	Msa0823370:CDS
AATTTGAAGTGAAGTGAATT+GGG	0.459009	5_3:-62036576	None:intergenic
TTATTCGGTTTCATCCATAA+AGG	0.468761	5_3:+62036707	Msa0823370:CDS
GGCATTGAGACTGAGATAAC+CGG	0.470227	5_3:-62036783	None:intergenic
GTCTCAATGCCTTTGGGAAG+CGG	0.473844	5_3:+62036795	Msa0823370:CDS
GCTTCTCTCGCCGGCGGCGT+CGG	0.474606	5_3:+62036737	Msa0823370:CDS
ATGTATTATGACCTATTACT+TGG	0.475469	5_3:+62038549	Msa0823370:three_prime_UTR
GACTGCATTGTTCTGTAAGC+AGG	0.484846	5_3:+62038411	Msa0823370:three_prime_UTR
TTCAGATGTATTATTGTACT+TGG	0.494127	5_3:+62038323	Msa0823370:three_prime_UTR
CAGTCAGCCTTAGCAGTAGC+AGG	0.498958	5_3:-62038253	None:intergenic
AGGTGTACTAACATGGATCA+TGG	0.511770	5_3:+62037244	Msa0823370:CDS
GGATTGTTATTGGTCGGTGG+AGG	0.525170	5_3:+62036683	Msa0823370:CDS
TTCTGTTTCACAATTCCGTA+CGG	0.532838	5_3:+62036662	Msa0823370:CDS
GGATCTCGATGGCGAGAGCA+AGG	0.538621	5_3:-62036829	None:intergenic
ACATCTAAGATTATGCCTGC+TGG	0.542306	5_3:+62037284	Msa0823370:CDS
CTGGTATGATTGCAGGACTC+AGG	0.544138	5_3:+62037303	Msa0823370:CDS
TCATATACCTGCTACTGCTA+AGG	0.552394	5_3:+62038246	Msa0823370:CDS
TGTTGCAAAACAGTTTGTGC+AGG	0.563627	5_3:+62037224	Msa0823370:intron
CGACCCGGTTCCGACGCCGC+CGG	0.568570	5_3:-62036747	None:intergenic
GGCGAGGATGAGAAGCGACC+CGG	0.569011	5_3:-62036762	None:intergenic
CCGTACGGATTGTTATTGGT+CGG	0.572524	5_3:+62036677	Msa0823370:CDS
AGTCCTGCAATCATACCAGC+AGG	0.578660	5_3:-62037299	None:intergenic
GGAAGTACCGCTTCTCTCGC+CGG	0.585532	5_3:+62036728	Msa0823370:CDS
TACGGATTGTTATTGGTCGG+TGG	0.593122	5_3:+62036680	Msa0823370:CDS
GACGCCGCCGGCGAGAGAAG+CGG	0.604749	5_3:-62036735	None:intergenic
GGTGTACTAACATGGATCAT+GGG	0.605410	5_3:+62037245	Msa0823370:CDS
GGGTCGCTTCTCATCCTCGC+CGG	0.607904	5_3:+62036764	Msa0823370:CDS
ATAAAAGGTGCAATCACAGA+AGG	0.610573	5_3:-62038593	None:intergenic
TTTGTGCAGGTGTACTAACA+TGG	0.620050	5_3:+62037237	Msa0823370:CDS
TGTGTATACTTTGAGGCGTG+TGG	0.624181	5_3:-62036514	None:intergenic
CCGACCAATAACAATCCGTA+CGG	0.654910	5_3:-62036677	None:intergenic
AAAGCGCATACGGATCTCGA+TGG	0.662369	5_3:-62036840	None:intergenic
CTGTACAAACTAGCAACTGG+TGG	0.664442	5_3:+62038220	Msa0823370:CDS
TTGAGGCGTGTGGAATACAC+AGG	0.685817	5_3:-62036504	None:intergenic
GAAGTGAATTGGGACAACGA+TGG	0.708088	5_3:-62036566	None:intergenic
AGTACCGCTTCTCTCGCCGG+CGG	0.718006	5_3:+62036731	Msa0823370:CDS
TGAGACTGAGATAACCGGCG+AGG	0.730012	5_3:-62036778	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTAATTTCTGAAATAATATT+TGG	-	chr5_3:62037962-62037981	None:intergenic	10.0%
!!!	TTAATTTCTGAAATAATATT+TGG	-	chr5_3:62037962-62037981	None:intergenic	10.0%
!!	TTTAATGCATATTACTTATA+AGG	-	chr5_3:62037013-62037032	None:intergenic	15.0%
!!	AATAGTATTATTTACATGTT+TGG	-	chr5_3:62037199-62037218	None:intergenic	15.0%
!!	TTAATACAAGATTTAGTTAT+TGG	+	chr5_3:62037380-62037399	Msa0823370:intron	15.0%
!!	ATTCTGTAACAAAAAAAAAA+AGG	+	chr5_3:62037403-62037422	Msa0823370:intron	15.0%
!!	TTCTGTAACAAAAAAAAAAA+GGG	+	chr5_3:62037404-62037423	Msa0823370:intron	15.0%
!!	TAGATAGAATTTATAAAGTT+TGG	+	chr5_3:62037656-62037675	Msa0823370:intron	15.0%
!!	AGATAGAATTTATAAAGTTT+GGG	+	chr5_3:62037657-62037676	Msa0823370:intron	15.0%
!!	AACTTTATAAACTTTGATTA+GGG	-	chr5_3:62037820-62037839	None:intergenic	15.0%
!!	AAACTTTATAAACTTTGATT+AGG	-	chr5_3:62037821-62037840	None:intergenic	15.0%
!!!	AATCAAAGTTTATAAAGTTT+TGG	+	chr5_3:62037821-62037840	Msa0823370:intron	15.0%
!!	TGTTTAATATATAAGAGAAT+TGG	+	chr5_3:62038384-62038403	Msa0823370:three_prime_UTR	15.0%
!!	TTTAATGCATATTACTTATA+AGG	-	chr5_3:62037013-62037032	None:intergenic	15.0%
!!	AATAGTATTATTTACATGTT+TGG	-	chr5_3:62037199-62037218	None:intergenic	15.0%
!!	TTAATACAAGATTTAGTTAT+TGG	+	chr5_3:62037380-62037399	Msa0823370:intron	15.0%
!!	ATTCTGTAACAAAAAAAAAA+AGG	+	chr5_3:62037403-62037422	Msa0823370:intron	15.0%
!!	TTCTGTAACAAAAAAAAAAA+GGG	+	chr5_3:62037404-62037423	Msa0823370:intron	15.0%
!!	TAGATAGAATTTATAAAGTT+TGG	+	chr5_3:62037656-62037675	Msa0823370:intron	15.0%
!!	AGATAGAATTTATAAAGTTT+GGG	+	chr5_3:62037657-62037676	Msa0823370:intron	15.0%
!!	AACTTTATAAACTTTGATTA+GGG	-	chr5_3:62037820-62037839	None:intergenic	15.0%
!!	AAACTTTATAAACTTTGATT+AGG	-	chr5_3:62037821-62037840	None:intergenic	15.0%
!!!	AATCAAAGTTTATAAAGTTT+TGG	+	chr5_3:62037821-62037840	Msa0823370:intron	15.0%
!!	TGTTTAATATATAAGAGAAT+TGG	+	chr5_3:62038384-62038403	Msa0823370:three_prime_UTR	15.0%
!!	ATTTGTGAGAAAAGAAAATT+TGG	-	chr5_3:62036605-62036624	None:intergenic	20.0%
!!!	TTGAGTTTAAATGTCAATTT+TGG	+	chr5_3:62037145-62037164	Msa0823370:intron	20.0%
!!!	AAGTACTTAGTTTTATTTGT+AGG	+	chr5_3:62037344-62037363	Msa0823370:intron	20.0%
!!	AAAAAAAAGGGATTTATTGT+TGG	+	chr5_3:62037416-62037435	Msa0823370:intron	20.0%
!!	AATGCATTAACTATTCATAA+AGG	-	chr5_3:62037482-62037501	None:intergenic	20.0%
!!!	TCTTTTATTCTGAAAGTAAA+TGG	-	chr5_3:62037567-62037586	None:intergenic	20.0%
!!	TATAATACGTATATGTTAGA+TGG	+	chr5_3:62038300-62038319	Msa0823370:three_prime_UTR	20.0%
!!	ATTTGTGAGAAAAGAAAATT+TGG	-	chr5_3:62036605-62036624	None:intergenic	20.0%
!!!	TTGAGTTTAAATGTCAATTT+TGG	+	chr5_3:62037145-62037164	Msa0823370:intron	20.0%
!!!	AAGTACTTAGTTTTATTTGT+AGG	+	chr5_3:62037344-62037363	Msa0823370:intron	20.0%
!!	AAAAAAAAGGGATTTATTGT+TGG	+	chr5_3:62037416-62037435	Msa0823370:intron	20.0%
!!	AATGCATTAACTATTCATAA+AGG	-	chr5_3:62037482-62037501	None:intergenic	20.0%
!!!	TCTTTTATTCTGAAAGTAAA+TGG	-	chr5_3:62037567-62037586	None:intergenic	20.0%
!!	TATAATACGTATATGTTAGA+TGG	+	chr5_3:62038300-62038319	Msa0823370:three_prime_UTR	20.0%
!!	AATTTGAAGTGAAGTGAATT+GGG	-	chr5_3:62036579-62036598	None:intergenic	25.0%
!!!	TGATTTTGATTCGTATTTTG+TGG	+	chr5_3:62036944-62036963	Msa0823370:intron	25.0%
!	TGCACATTAAATAAAAATGC+AGG	-	chr5_3:62037057-62037076	None:intergenic	25.0%
!!!	TCAAGTGATCTATTTTTGTT+TGG	+	chr5_3:62037111-62037130	Msa0823370:intron	25.0%
!!	AGTTTAAATGTCAATTTTGG+AGG	+	chr5_3:62037148-62037167	Msa0823370:intron	25.0%
!	CATAATCATTTCTATTCTAG+AGG	+	chr5_3:62037171-62037190	Msa0823370:intron	25.0%
!	ATGAATAGTTAATGCATTCA+CGG	+	chr5_3:62037484-62037503	Msa0823370:intron	25.0%
!!!	ACTATTTTTCTAGCTAATGA+AGG	+	chr5_3:62037529-62037548	Msa0823370:intron	25.0%
!!	TATTCTGAAAGTAAATGGTA+TGG	-	chr5_3:62037562-62037581	None:intergenic	25.0%
!	TTCAGATGTATTATTGTACT+TGG	+	chr5_3:62038323-62038342	Msa0823370:three_prime_UTR	25.0%
!	AGGTCATAATACATTTCATT+TGG	-	chr5_3:62038543-62038562	None:intergenic	25.0%
!	ATGTATTATGACCTATTACT+TGG	+	chr5_3:62038549-62038568	Msa0823370:three_prime_UTR	25.0%
!	TATGCCAGATTTCATATAAA+AGG	-	chr5_3:62038611-62038630	None:intergenic	25.0%
!!	AATTTGAAGTGAAGTGAATT+GGG	-	chr5_3:62036579-62036598	None:intergenic	25.0%
!!!	TGATTTTGATTCGTATTTTG+TGG	+	chr5_3:62036944-62036963	Msa0823370:intron	25.0%
!	TGCACATTAAATAAAAATGC+AGG	-	chr5_3:62037057-62037076	None:intergenic	25.0%
!!!	TCAAGTGATCTATTTTTGTT+TGG	+	chr5_3:62037111-62037130	Msa0823370:intron	25.0%
!!	AGTTTAAATGTCAATTTTGG+AGG	+	chr5_3:62037148-62037167	Msa0823370:intron	25.0%
!	CATAATCATTTCTATTCTAG+AGG	+	chr5_3:62037171-62037190	Msa0823370:intron	25.0%
!	ATGAATAGTTAATGCATTCA+CGG	+	chr5_3:62037484-62037503	Msa0823370:intron	25.0%
!!!	ACTATTTTTCTAGCTAATGA+AGG	+	chr5_3:62037529-62037548	Msa0823370:intron	25.0%
!!	TATTCTGAAAGTAAATGGTA+TGG	-	chr5_3:62037562-62037581	None:intergenic	25.0%
!	TTCAGATGTATTATTGTACT+TGG	+	chr5_3:62038323-62038342	Msa0823370:three_prime_UTR	25.0%
!	AGGTCATAATACATTTCATT+TGG	-	chr5_3:62038543-62038562	None:intergenic	25.0%
!	ATGTATTATGACCTATTACT+TGG	+	chr5_3:62038549-62038568	Msa0823370:three_prime_UTR	25.0%
!	TATGCCAGATTTCATATAAA+AGG	-	chr5_3:62038611-62038630	None:intergenic	25.0%
!	GAATTTGAAGTGAAGTGAAT+TGG	-	chr5_3:62036580-62036599	None:intergenic	30.0%
	TTATTCGGTTTCATCCATAA+AGG	+	chr5_3:62036707-62036726	Msa0823370:CDS	30.0%
!	TGAAATGAAAAAAGCGCATA+CGG	-	chr5_3:62036853-62036872	None:intergenic	30.0%
	ATTCATAAAGGACAAGTTCA+TGG	-	chr5_3:62037470-62037489	None:intergenic	30.0%
	ACTTATTCTAATTGTGCTGT+TGG	+	chr5_3:62037755-62037774	Msa0823370:intron	30.0%
!	CTTATTCTAATTGTGCTGTT+GGG	+	chr5_3:62037756-62037775	Msa0823370:intron	30.0%
	AAAGAACTTGCACTAAACAA+GGG	-	chr5_3:62037888-62037907	None:intergenic	30.0%
	AAAAGAACTTGCACTAAACA+AGG	-	chr5_3:62037889-62037908	None:intergenic	30.0%
!!	TGTTTGTTTGCTAGTCTTAA+TGG	+	chr5_3:62038006-62038025	Msa0823370:intron	30.0%
	TTGTAAGCATTATGCAAGTT+TGG	+	chr5_3:62038056-62038075	Msa0823370:intron	30.0%
!	TGCACCTTTTATATGAAATC+TGG	+	chr5_3:62038604-62038623	Msa0823370:three_prime_UTR	30.0%
	ACATTGTCAGCATTTAGATT+AGG	+	chr5_3:62038635-62038654	Msa0823370:three_prime_UTR	30.0%
!	GAATTTGAAGTGAAGTGAAT+TGG	-	chr5_3:62036580-62036599	None:intergenic	30.0%
	TTATTCGGTTTCATCCATAA+AGG	+	chr5_3:62036707-62036726	Msa0823370:CDS	30.0%
!	TGAAATGAAAAAAGCGCATA+CGG	-	chr5_3:62036853-62036872	None:intergenic	30.0%
	ATTCATAAAGGACAAGTTCA+TGG	-	chr5_3:62037470-62037489	None:intergenic	30.0%
	ACTTATTCTAATTGTGCTGT+TGG	+	chr5_3:62037755-62037774	Msa0823370:intron	30.0%
!	CTTATTCTAATTGTGCTGTT+GGG	+	chr5_3:62037756-62037775	Msa0823370:intron	30.0%
	AAAGAACTTGCACTAAACAA+GGG	-	chr5_3:62037888-62037907	None:intergenic	30.0%
	AAAAGAACTTGCACTAAACA+AGG	-	chr5_3:62037889-62037908	None:intergenic	30.0%
!!	TGTTTGTTTGCTAGTCTTAA+TGG	+	chr5_3:62038006-62038025	Msa0823370:intron	30.0%
	TTGTAAGCATTATGCAAGTT+TGG	+	chr5_3:62038056-62038075	Msa0823370:intron	30.0%
!	TGCACCTTTTATATGAAATC+TGG	+	chr5_3:62038604-62038623	Msa0823370:three_prime_UTR	30.0%
	ACATTGTCAGCATTTAGATT+AGG	+	chr5_3:62038635-62038654	Msa0823370:three_prime_UTR	30.0%
	CAGAGAATGTGTATACTTTG+AGG	-	chr5_3:62036524-62036543	None:intergenic	35.0%
	TTCTGTTTCACAATTCCGTA+CGG	+	chr5_3:62036662-62036681	Msa0823370:CDS	35.0%
!	AATTCCGTACGGATTGTTAT+TGG	+	chr5_3:62036673-62036692	Msa0823370:CDS	35.0%
!	TGCATTCACGGATGTATTTT+TGG	+	chr5_3:62037496-62037515	Msa0823370:intron	35.0%
!	GACTTGAGTTTTTCTCCTAA+GGG	-	chr5_3:62037613-62037632	None:intergenic	35.0%
!	AGACTTGAGTTTTTCTCCTA+AGG	-	chr5_3:62037614-62037633	None:intergenic	35.0%
	GAAAAACTCAAGTCTCAAGT+TGG	+	chr5_3:62037619-62037638	Msa0823370:intron	35.0%
!!!	ACGCCTTACAAGTTGATTTT+AGG	+	chr5_3:62037688-62037707	Msa0823370:intron	35.0%
!!!	CGCCTTACAAGTTGATTTTA+GGG	+	chr5_3:62037689-62037708	Msa0823370:intron	35.0%
!!!	GCCTTACAAGTTGATTTTAG+GGG	+	chr5_3:62037690-62037709	Msa0823370:intron	35.0%
	ACCCCTAAAATCAACTTGTA+AGG	-	chr5_3:62037694-62037713	None:intergenic	35.0%
	TTGTGTCGAATTGTAAAGTG+AGG	-	chr5_3:62037851-62037870	None:intergenic	35.0%
	CAATGCACATTCATAAGTAG+CGG	-	chr5_3:62038103-62038122	None:intergenic	35.0%
!!	GCAACGTATGTTTTTGTATC+TGG	+	chr5_3:62038142-62038161	Msa0823370:intron	35.0%
!	TTCCTTTTCTTTTTGTGCAG+TGG	+	chr5_3:62038178-62038197	Msa0823370:intron	35.0%
	TATCTGTACAAACTAGCAAC+TGG	+	chr5_3:62038217-62038236	Msa0823370:CDS	35.0%
!	TACTTGGTGATTTCAGAGTT+TGG	+	chr5_3:62038339-62038358	Msa0823370:three_prime_UTR	35.0%
	TTCGTGAAGAAAAAGAGGAA+AGG	-	chr5_3:62038451-62038470	None:intergenic	35.0%
	AATGCTTCGTGAAGAAAAAG+AGG	-	chr5_3:62038456-62038475	None:intergenic	35.0%
!	TCTTTTTCTTCACGAAGCAT+TGG	+	chr5_3:62038455-62038474	Msa0823370:three_prime_UTR	35.0%
!!	CTTTTTCTTCACGAAGCATT+GGG	+	chr5_3:62038456-62038475	Msa0823370:three_prime_UTR	35.0%
!!!	TTTTTCTTCACGAAGCATTG+GGG	+	chr5_3:62038457-62038476	Msa0823370:three_prime_UTR	35.0%
!	ATAAAAGGTGCAATCACAGA+AGG	-	chr5_3:62038596-62038615	None:intergenic	35.0%
	CAGAGAATGTGTATACTTTG+AGG	-	chr5_3:62036524-62036543	None:intergenic	35.0%
	TTCTGTTTCACAATTCCGTA+CGG	+	chr5_3:62036662-62036681	Msa0823370:CDS	35.0%
!	AATTCCGTACGGATTGTTAT+TGG	+	chr5_3:62036673-62036692	Msa0823370:CDS	35.0%
!	TGCATTCACGGATGTATTTT+TGG	+	chr5_3:62037496-62037515	Msa0823370:intron	35.0%
!	GACTTGAGTTTTTCTCCTAA+GGG	-	chr5_3:62037613-62037632	None:intergenic	35.0%
!	AGACTTGAGTTTTTCTCCTA+AGG	-	chr5_3:62037614-62037633	None:intergenic	35.0%
	GAAAAACTCAAGTCTCAAGT+TGG	+	chr5_3:62037619-62037638	Msa0823370:intron	35.0%
!!!	ACGCCTTACAAGTTGATTTT+AGG	+	chr5_3:62037688-62037707	Msa0823370:intron	35.0%
!!!	CGCCTTACAAGTTGATTTTA+GGG	+	chr5_3:62037689-62037708	Msa0823370:intron	35.0%
!!!	GCCTTACAAGTTGATTTTAG+GGG	+	chr5_3:62037690-62037709	Msa0823370:intron	35.0%
	ACCCCTAAAATCAACTTGTA+AGG	-	chr5_3:62037694-62037713	None:intergenic	35.0%
	TTGTGTCGAATTGTAAAGTG+AGG	-	chr5_3:62037851-62037870	None:intergenic	35.0%
	CAATGCACATTCATAAGTAG+CGG	-	chr5_3:62038103-62038122	None:intergenic	35.0%
!!	GCAACGTATGTTTTTGTATC+TGG	+	chr5_3:62038142-62038161	Msa0823370:intron	35.0%
!	TTCCTTTTCTTTTTGTGCAG+TGG	+	chr5_3:62038178-62038197	Msa0823370:intron	35.0%
	TATCTGTACAAACTAGCAAC+TGG	+	chr5_3:62038217-62038236	Msa0823370:CDS	35.0%
!	TACTTGGTGATTTCAGAGTT+TGG	+	chr5_3:62038339-62038358	Msa0823370:three_prime_UTR	35.0%
	TTCGTGAAGAAAAAGAGGAA+AGG	-	chr5_3:62038451-62038470	None:intergenic	35.0%
	AATGCTTCGTGAAGAAAAAG+AGG	-	chr5_3:62038456-62038475	None:intergenic	35.0%
!	TCTTTTTCTTCACGAAGCAT+TGG	+	chr5_3:62038455-62038474	Msa0823370:three_prime_UTR	35.0%
!!	CTTTTTCTTCACGAAGCATT+GGG	+	chr5_3:62038456-62038475	Msa0823370:three_prime_UTR	35.0%
!!!	TTTTTCTTCACGAAGCATTG+GGG	+	chr5_3:62038457-62038476	Msa0823370:three_prime_UTR	35.0%
!	ATAAAAGGTGCAATCACAGA+AGG	-	chr5_3:62038596-62038615	None:intergenic	35.0%
	CAACGATGGAGAAAAAGTCT+TGG	-	chr5_3:62036555-62036574	None:intergenic	40.0%
	TATCTCAGTCTCAATGCCTT+TGG	+	chr5_3:62036788-62036807	Msa0823370:CDS	40.0%
	ATCTCAGTCTCAATGCCTTT+GGG	+	chr5_3:62036789-62036808	Msa0823370:CDS	40.0%
	TGTTGCAAAACAGTTTGTGC+AGG	+	chr5_3:62037224-62037243	Msa0823370:intron	40.0%
	TTTGTGCAGGTGTACTAACA+TGG	+	chr5_3:62037237-62037256	Msa0823370:CDS	40.0%
	AGGTGTACTAACATGGATCA+TGG	+	chr5_3:62037244-62037263	Msa0823370:CDS	40.0%
	GGTGTACTAACATGGATCAT+GGG	+	chr5_3:62037245-62037264	Msa0823370:CDS	40.0%
!	ACATCTAAGATTATGCCTGC+TGG	+	chr5_3:62037284-62037303	Msa0823370:CDS	40.0%
	GCAGGACTCAGGTAATTAAA+AGG	+	chr5_3:62037314-62037333	Msa0823370:intron	40.0%
	AGTTTGCGATATCATCCCTT+AGG	+	chr5_3:62037595-62037614	Msa0823370:intron	40.0%
!!	ACTCAAGTCTCAAGTTGGTT+AGG	+	chr5_3:62037624-62037643	Msa0823370:intron	40.0%
!!	CTCAAGTCTCAAGTTGGTTA+GGG	+	chr5_3:62037625-62037644	Msa0823370:intron	40.0%
	ATTAGGGCTATTGTGTTGTG+TGG	-	chr5_3:62037804-62037823	None:intergenic	40.0%
	GACCACTGCACAAAAAGAAA+AGG	-	chr5_3:62038183-62038202	None:intergenic	40.0%
	TCATATACCTGCTACTGCTA+AGG	+	chr5_3:62038246-62038265	Msa0823370:CDS	40.0%
	TATGCGTCTGTCTTGCATTT+AGG	-	chr5_3:62038487-62038506	None:intergenic	40.0%
	TTGCACAGTAGCCAAGTAAT+AGG	-	chr5_3:62038563-62038582	None:intergenic	40.0%
	CAACGATGGAGAAAAAGTCT+TGG	-	chr5_3:62036555-62036574	None:intergenic	40.0%
	TATCTCAGTCTCAATGCCTT+TGG	+	chr5_3:62036788-62036807	Msa0823370:CDS	40.0%
	ATCTCAGTCTCAATGCCTTT+GGG	+	chr5_3:62036789-62036808	Msa0823370:CDS	40.0%
	TGTTGCAAAACAGTTTGTGC+AGG	+	chr5_3:62037224-62037243	Msa0823370:intron	40.0%
	TTTGTGCAGGTGTACTAACA+TGG	+	chr5_3:62037237-62037256	Msa0823370:CDS	40.0%
	AGGTGTACTAACATGGATCA+TGG	+	chr5_3:62037244-62037263	Msa0823370:CDS	40.0%
	GGTGTACTAACATGGATCAT+GGG	+	chr5_3:62037245-62037264	Msa0823370:CDS	40.0%
!	ACATCTAAGATTATGCCTGC+TGG	+	chr5_3:62037284-62037303	Msa0823370:CDS	40.0%
	GCAGGACTCAGGTAATTAAA+AGG	+	chr5_3:62037314-62037333	Msa0823370:intron	40.0%
	AGTTTGCGATATCATCCCTT+AGG	+	chr5_3:62037595-62037614	Msa0823370:intron	40.0%
!!	ACTCAAGTCTCAAGTTGGTT+AGG	+	chr5_3:62037624-62037643	Msa0823370:intron	40.0%
!!	CTCAAGTCTCAAGTTGGTTA+GGG	+	chr5_3:62037625-62037644	Msa0823370:intron	40.0%
	ATTAGGGCTATTGTGTTGTG+TGG	-	chr5_3:62037804-62037823	None:intergenic	40.0%
	GACCACTGCACAAAAAGAAA+AGG	-	chr5_3:62038183-62038202	None:intergenic	40.0%
	TCATATACCTGCTACTGCTA+AGG	+	chr5_3:62038246-62038265	Msa0823370:CDS	40.0%
	TATGCGTCTGTCTTGCATTT+AGG	-	chr5_3:62038487-62038506	None:intergenic	40.0%
	TTGCACAGTAGCCAAGTAAT+AGG	-	chr5_3:62038563-62038582	None:intergenic	40.0%
!!	TGTGTATACTTTGAGGCGTG+TGG	-	chr5_3:62036517-62036536	None:intergenic	45.0%
	GAAGTGAATTGGGACAACGA+TGG	-	chr5_3:62036569-62036588	None:intergenic	45.0%
	CCGACCAATAACAATCCGTA+CGG	-	chr5_3:62036680-62036699	None:intergenic	45.0%
!	CCGTACGGATTGTTATTGGT+CGG	+	chr5_3:62036677-62036696	Msa0823370:CDS	45.0%
!	TACGGATTGTTATTGGTCGG+TGG	+	chr5_3:62036680-62036699	Msa0823370:CDS	45.0%
	TTGGTCGGTGGAGGATTATT+CGG	+	chr5_3:62036692-62036711	Msa0823370:CDS	45.0%
	GAGAAGCGGTACTTCCTTTA+TGG	-	chr5_3:62036724-62036743	None:intergenic	45.0%
!	GGCATTGAGACTGAGATAAC+CGG	-	chr5_3:62036786-62036805	None:intergenic	45.0%
!	AGAGTTTTTCCGCTTCCCAA+AGG	-	chr5_3:62036807-62036826	None:intergenic	45.0%
	AACTTGCACTAAACAAGGGC+AGG	-	chr5_3:62037884-62037903	None:intergenic	45.0%
	CTGTACAAACTAGCAACTGG+TGG	+	chr5_3:62038220-62038239	Msa0823370:CDS	45.0%
	GACTGCATTGTTCTGTAAGC+AGG	+	chr5_3:62038411-62038430	Msa0823370:three_prime_UTR	45.0%
	GGTGCAATCACAGAAGGAAA+TGG	-	chr5_3:62038590-62038609	None:intergenic	45.0%
!!	TGTGTATACTTTGAGGCGTG+TGG	-	chr5_3:62036517-62036536	None:intergenic	45.0%
	GAAGTGAATTGGGACAACGA+TGG	-	chr5_3:62036569-62036588	None:intergenic	45.0%
	CCGACCAATAACAATCCGTA+CGG	-	chr5_3:62036680-62036699	None:intergenic	45.0%
!	CCGTACGGATTGTTATTGGT+CGG	+	chr5_3:62036677-62036696	Msa0823370:CDS	45.0%
!	TACGGATTGTTATTGGTCGG+TGG	+	chr5_3:62036680-62036699	Msa0823370:CDS	45.0%
	TTGGTCGGTGGAGGATTATT+CGG	+	chr5_3:62036692-62036711	Msa0823370:CDS	45.0%
	GAGAAGCGGTACTTCCTTTA+TGG	-	chr5_3:62036724-62036743	None:intergenic	45.0%
!	GGCATTGAGACTGAGATAAC+CGG	-	chr5_3:62036786-62036805	None:intergenic	45.0%
!	AGAGTTTTTCCGCTTCCCAA+AGG	-	chr5_3:62036807-62036826	None:intergenic	45.0%
	AACTTGCACTAAACAAGGGC+AGG	-	chr5_3:62037884-62037903	None:intergenic	45.0%
	CTGTACAAACTAGCAACTGG+TGG	+	chr5_3:62038220-62038239	Msa0823370:CDS	45.0%
	GACTGCATTGTTCTGTAAGC+AGG	+	chr5_3:62038411-62038430	Msa0823370:three_prime_UTR	45.0%
	GGTGCAATCACAGAAGGAAA+TGG	-	chr5_3:62038590-62038609	None:intergenic	45.0%
!	GGATTGTTATTGGTCGGTGG+AGG	+	chr5_3:62036683-62036702	Msa0823370:CDS	50.0%
!	GTCTCAATGCCTTTGGGAAG+CGG	+	chr5_3:62036795-62036814	Msa0823370:CDS	50.0%
	AAAGCGCATACGGATCTCGA+TGG	-	chr5_3:62036843-62036862	None:intergenic	50.0%
	ATGCCTGCTGGTATGATTGC+AGG	+	chr5_3:62037296-62037315	Msa0823370:CDS	50.0%
	AGTCCTGCAATCATACCAGC+AGG	-	chr5_3:62037302-62037321	None:intergenic	50.0%
	CTGGTATGATTGCAGGACTC+AGG	+	chr5_3:62037303-62037322	Msa0823370:CDS	50.0%
!	GGATTGTTATTGGTCGGTGG+AGG	+	chr5_3:62036683-62036702	Msa0823370:CDS	50.0%
!	GTCTCAATGCCTTTGGGAAG+CGG	+	chr5_3:62036795-62036814	Msa0823370:CDS	50.0%
	AAAGCGCATACGGATCTCGA+TGG	-	chr5_3:62036843-62036862	None:intergenic	50.0%
	ATGCCTGCTGGTATGATTGC+AGG	+	chr5_3:62037296-62037315	Msa0823370:CDS	50.0%
	AGTCCTGCAATCATACCAGC+AGG	-	chr5_3:62037302-62037321	None:intergenic	50.0%
	CTGGTATGATTGCAGGACTC+AGG	+	chr5_3:62037303-62037322	Msa0823370:CDS	50.0%
	TGAGACTGAGATAACCGGCG+AGG	-	chr5_3:62036781-62036800	None:intergenic	55.0%
	CAGTCAGCCTTAGCAGTAGC+AGG	-	chr5_3:62038256-62038275	None:intergenic	55.0%
	TGAGACTGAGATAACCGGCG+AGG	-	chr5_3:62036781-62036800	None:intergenic	55.0%
	CAGTCAGCCTTAGCAGTAGC+AGG	-	chr5_3:62038256-62038275	None:intergenic	55.0%
!!	GGAAGTACCGCTTCTCTCGC+CGG	+	chr5_3:62036728-62036747	Msa0823370:CDS	60.0%
	GGATCTCGATGGCGAGAGCA+AGG	-	chr5_3:62036832-62036851	None:intergenic	60.0%
!!	GGAAGTACCGCTTCTCTCGC+CGG	+	chr5_3:62036728-62036747	Msa0823370:CDS	60.0%
	GGATCTCGATGGCGAGAGCA+AGG	-	chr5_3:62036832-62036851	None:intergenic	60.0%
	AGTACCGCTTCTCTCGCCGG+CGG	+	chr5_3:62036731-62036750	Msa0823370:CDS	65.0%
	GGCGAGGATGAGAAGCGACC+CGG	-	chr5_3:62036765-62036784	None:intergenic	65.0%
	GGGTCGCTTCTCATCCTCGC+CGG	+	chr5_3:62036764-62036783	Msa0823370:CDS	65.0%
	AGTACCGCTTCTCTCGCCGG+CGG	+	chr5_3:62036731-62036750	Msa0823370:CDS	65.0%
	GGCGAGGATGAGAAGCGACC+CGG	-	chr5_3:62036765-62036784	None:intergenic	65.0%
	GGGTCGCTTCTCATCCTCGC+CGG	+	chr5_3:62036764-62036783	Msa0823370:CDS	65.0%
	GACGCCGCCGGCGAGAGAAG+CGG	-	chr5_3:62036738-62036757	None:intergenic	75.0%
	GCTTCTCTCGCCGGCGGCGT+CGG	+	chr5_3:62036737-62036756	Msa0823370:CDS	75.0%
	GACGCCGCCGGCGAGAGAAG+CGG	-	chr5_3:62036738-62036757	None:intergenic	75.0%
	GCTTCTCTCGCCGGCGGCGT+CGG	+	chr5_3:62036737-62036756	Msa0823370:CDS	75.0%
!!	CTCGCCGGCGGCGTCGGAAC+CGG	+	chr5_3:62036743-62036762	Msa0823370:CDS	80.0%
!!	TCGCCGGCGGCGTCGGAACC+GGG	+	chr5_3:62036744-62036763	Msa0823370:CDS	80.0%
!	CGACCCGGTTCCGACGCCGC+CGG	-	chr5_3:62036750-62036769	None:intergenic	80.0%
!!	CTCGCCGGCGGCGTCGGAAC+CGG	+	chr5_3:62036743-62036762	Msa0823370:CDS	80.0%
!!	TCGCCGGCGGCGTCGGAACC+GGG	+	chr5_3:62036744-62036763	Msa0823370:CDS	80.0%
!	CGACCCGGTTCCGACGCCGC+CGG	-	chr5_3:62036750-62036769	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	62036507	62038668	62036507	ID=Msa0823370;Name=Msa0823370
chr5_3	mRNA	62036507	62038668	62036507	ID=Msa0823370-mRNA-1;Parent=Msa0823370;Name=Msa0823370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=146\|1\|1\|1\|1\|1\|3\|392\|121
chr5_3	exon	62036507	62036851	62036507	ID=Msa0823370-mRNA-1:exon:19233;Parent=Msa0823370-mRNA-1
chr5_3	exon	62037237	62037324	62037237	ID=Msa0823370-mRNA-1:exon:19234;Parent=Msa0823370-mRNA-1
chr5_3	exon	62038198	62038668	62038198	ID=Msa0823370-mRNA-1:exon:19235;Parent=Msa0823370-mRNA-1
chr5_3	five_prime_UTR	62036507	62036652	62036507	ID=Msa0823370-mRNA-1:five_prime_utr;Parent=Msa0823370-mRNA-1
chr5_3	CDS	62036653	62036851	62036653	ID=Msa0823370-mRNA-1:cds;Parent=Msa0823370-mRNA-1
chr5_3	CDS	62037237	62037324	62037237	ID=Msa0823370-mRNA-1:cds;Parent=Msa0823370-mRNA-1
chr5_3	CDS	62038198	62038276	62038198	ID=Msa0823370-mRNA-1:cds;Parent=Msa0823370-mRNA-1
chr5_3	three_prime_UTR	62038277	62038668	62038277	ID=Msa0823370-mRNA-1:three_prime_utr;Parent=Msa0823370-mRNA-1

Gene Sequence	>Msa0823370 ATGCACGATTTCTGTTTCACAATTCCGTACGGATTGTTATTGGTCGGTGGAGGATTATTCGGTTTCATCCATAAAGGAAGTACCGCTTCTCTCGCCGGCGGCGTCGGAACCGGGTCGCTTCTCATCCTCGCCGGTTATCTCAGTCTCAATGCCTTTGGGAAGCGGAAAAACTCTTACCTTGCTCTCGCCATCGAGATCCTTTGTGCAGGTGTACTAACATGGATCATGGGACAACGTTACTTGCAAACATCTAAGATTATGCCTGCTGGTATGATTGCAGGACTCAGTGGTCTCATGACTCTGTTTTATCTGTACAAACTAGCAACTGGTGGCAATCATATACCTGCTACTGCTAAGGCTGACTGA
Protein sequence	>Msa0823370 MHDFCFTIPYGLLLVGGGLFGFIHKGSTASLAGGVGTGSLLILAGYLSLNAFGKRKNSYLALAIEILCAGVLTWIMGQRYLQTSKIMPAGMIAGLSGLMTLFYLYKLATGGNHIPATAKAD*