AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0742210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742210 MtrunA17_Chr5g0443741 97.521 121 3 0 1 121 1 121 2.35e-81 234
Msa0742210 MtrunA17_Chr7g0257051 37.815 119 74 0 1 119 107 225 1.07e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742210 AT1G50740.1 63.866 119 43 0 1 119 1 119 3.49e-53 163
Msa0742210 AT3G20510.1 64.706 119 42 0 1 119 1 119 9.25e-53 162
Msa0742210 AT3G57280.1 38.017 121 75 0 1 121 100 220 2.25e-17 75.1
Msa0742210 AT2G26240.1 40.196 102 55 2 6 107 9 104 6.53e-12 58.2

Find 56 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAAGCGGTACTTCCTTTA+TGG 0.180195 5_1:+62027519 None:intergenic
TATGCGTCTGTCTTGCATTT+AGG 0.241572 5_1:+62025734 None:intergenic
TATGCCAGATTTCATATAAA+AGG 0.246802 5_1:+62025612 None:intergenic
ATTTGTGAGAAAAGAAAATT+TGG 0.248706 5_1:+62027636 None:intergenic
CTCGCCGGCGGCGTCGGAAC+CGG 0.266751 5_1:-62027497 Msa0742210:CDS
AATTCCGTACGGATTGTTAT+TGG 0.288264 5_1:-62027567 Msa0742210:CDS
ATAGGTCAATACATTTCATT+TGG 0.340272 5_1:+62025678 None:intergenic
TACTTGGTGATTTCAGAGTT+TGG 0.354666 5_1:-62025879 Msa0742210:three_prime_UTR
TTGGTCGGTGGAGGATTATT+CGG 0.355132 5_1:-62027548 Msa0742210:CDS
TGTTTAATATATAAGAGAAT+TGG 0.362436 5_1:-62025834 Msa0742210:three_prime_UTR
TATCTCAGTCTCAATGCCTT+TGG 0.362461 5_1:-62027452 Msa0742210:CDS
ATCTCAGTCTCAATGCCTTT+GGG 0.363013 5_1:-62027451 Msa0742210:CDS
ATGCCTGCTGGTATGATTGC+AGG 0.367119 5_1:-62026921 Msa0742210:CDS
TCGCCGGCGGCGTCGGAACC+GGG 0.377853 5_1:-62027496 Msa0742210:CDS
GAATTTGAAGTGAAGTGAAT+TGG 0.386815 5_1:+62027661 None:intergenic
TTGCACAGTAGCCAAGTAAT+AGG 0.408980 5_1:+62025660 None:intergenic
TATAATACGTATATGTTAGA+TGG 0.409331 5_1:-62025918 Msa0742210:three_prime_UTR
AAATGTATTGACCTATTACT+TGG 0.444007 5_1:-62025671 Msa0742210:three_prime_UTR
GGTGCAATCACAGAAGGAAA+TGG 0.448983 5_1:+62025633 None:intergenic
TATCTGTACAAACTAGCAAC+TGG 0.454289 5_1:-62026001 Msa0742210:CDS
AATTTGAAGTGAAGTGAATT+GGG 0.459009 5_1:+62027662 None:intergenic
TTATTCGGTTTCATCCATAA+AGG 0.468761 5_1:-62027533 Msa0742210:CDS
GGCATTGAGACTGAGATAAC+CGG 0.470227 5_1:+62027457 None:intergenic
GTCTCAATGCCTTTGGGAAG+CGG 0.473844 5_1:-62027445 Msa0742210:CDS
GCTTCTCTCGCCGGCGGCGT+CGG 0.474606 5_1:-62027503 Msa0742210:CDS
GACTGCATTGTTCTGTAAGC+AGG 0.484846 5_1:-62025807 Msa0742210:three_prime_UTR
AATGTGTATACTTTACTTTG+AGG 0.488211 5_1:+62027723 None:intergenic
TTCAGATGTATTATTGTACT+TGG 0.494127 5_1:-62025895 Msa0742210:three_prime_UTR
CAGTCAGCCTTAGCAGTAGC+AGG 0.498958 5_1:+62025965 None:intergenic
AGGTGTACTAACATGGATCA+TGG 0.511770 5_1:-62026973 Msa0742210:CDS
GGATTGTTATTGGTCGGTGG+AGG 0.525170 5_1:-62027557 Msa0742210:CDS
TTCTGTTTCACAATTCCGTA+CGG 0.532838 5_1:-62027578 Msa0742210:CDS
GGATCTCGATGGCGAGAGCA+AGG 0.538621 5_1:+62027411 None:intergenic
ACATCTAAGATTATGCCTGC+TGG 0.542306 5_1:-62026933 Msa0742210:CDS
ATACTTTACTTTGAGGCGTG+TGG 0.543748 5_1:+62027730 None:intergenic
CTGGTATGATTGCAGGACTC+AGG 0.544138 5_1:-62026914 Msa0742210:intron
TCATATACCTGCTACTGCTA+AGG 0.552394 5_1:-62025972 Msa0742210:CDS
TGTTGCAAAACAGTTTGTGC+AGG 0.563627 5_1:-62026993 Msa0742210:intron
CGACCCGGTTCCGACGCCGC+CGG 0.568570 5_1:+62027493 None:intergenic
GGCGAGGATGAGAAGCGACC+CGG 0.569011 5_1:+62027478 None:intergenic
CCGTACGGATTGTTATTGGT+CGG 0.572524 5_1:-62027563 Msa0742210:CDS
AGTCCTGCAATCATACCAGC+AGG 0.578660 5_1:+62026918 None:intergenic
GGAAGTACCGCTTCTCTCGC+CGG 0.585532 5_1:-62027512 Msa0742210:CDS
TACGGATTGTTATTGGTCGG+TGG 0.593122 5_1:-62027560 Msa0742210:CDS
GACGCCGCCGGCGAGAGAAG+CGG 0.604749 5_1:+62027505 None:intergenic
GGTGTACTAACATGGATCAT+GGG 0.605410 5_1:-62026972 Msa0742210:CDS
GGGTCGCTTCTCATCCTCGC+CGG 0.607904 5_1:-62027476 Msa0742210:CDS
ATAAAAGGTGCAATCACAGA+AGG 0.610573 5_1:+62025627 None:intergenic
TTTGTGCAGGTGTACTAACA+TGG 0.620050 5_1:-62026980 Msa0742210:CDS
CCGACCAATAACAATCCGTA+CGG 0.654910 5_1:+62027563 None:intergenic
AAAGCGCATACGGATCTCGA+TGG 0.662369 5_1:+62027400 None:intergenic
CTGTACAAACTAGCAACTGG+TGG 0.664442 5_1:-62025998 Msa0742210:CDS
TTGAGGCGTGTGGAATACAC+AGG 0.685817 5_1:+62027740 None:intergenic
GAAGTGAATTGGGACAACGA+TGG 0.708088 5_1:+62027672 None:intergenic
AGTACCGCTTCTCTCGCCGG+CGG 0.718006 5_1:-62027509 Msa0742210:CDS
TGAGACTGAGATAACCGGCG+AGG 0.730012 5_1:+62027462 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTCTGAAATAATATT+TGG + chr5_1:62027055-62027074 None:intergenic 10.0%
!!! TTAATTTCTGAAATAATATT+TGG + chr5_1:62027055-62027074 None:intergenic 10.0%
!! TTTAATGCATATTACTTATA+AGG + chr5_1:62026084-62026103 None:intergenic 15.0%
!! AATAGTATTATTTACATGTT+TGG + chr5_1:62026293-62026312 None:intergenic 15.0%
!! TTAATACAAGATTTAGTTAT+TGG - chr5_1:62026474-62026493 Msa0742210:intron 15.0%
!! ATTCTGTAACAAAAAAAAAA+GGG - chr5_1:62026497-62026516 Msa0742210:intron 15.0%
!! TAGATAGAATTTATAAAGTT+TGG - chr5_1:62026749-62026768 Msa0742210:intron 15.0%
!! AGATAGAATTTATAAAGTTT+GGG - chr5_1:62026750-62026769 Msa0742210:intron 15.0%
!! AACTTTATAAACTTTGATTA+GGG + chr5_1:62026913-62026932 None:intergenic 15.0%
!! AAACTTTATAAACTTTGATT+AGG + chr5_1:62026914-62026933 None:intergenic 15.0%
!!! AATCAAAGTTTATAAAGTTT+TGG - chr5_1:62026914-62026933 Msa0742210:intron 15.0%
!! TGTTTAATATATAAGAGAAT+TGG - chr5_1:62027477-62027496 Msa0742210:CDS 15.0%
!! TTTAATGCATATTACTTATA+AGG + chr5_1:62026084-62026103 None:intergenic 15.0%
!! AATAGTATTATTTACATGTT+TGG + chr5_1:62026293-62026312 None:intergenic 15.0%
!! TTAATACAAGATTTAGTTAT+TGG - chr5_1:62026474-62026493 Msa0742210:intron 15.0%
!! ATTCTGTAACAAAAAAAAAA+GGG - chr5_1:62026497-62026516 Msa0742210:intron 15.0%
!! TAGATAGAATTTATAAAGTT+TGG - chr5_1:62026749-62026768 Msa0742210:intron 15.0%
!! AGATAGAATTTATAAAGTTT+GGG - chr5_1:62026750-62026769 Msa0742210:intron 15.0%
!! AACTTTATAAACTTTGATTA+GGG + chr5_1:62026913-62026932 None:intergenic 15.0%
!! AAACTTTATAAACTTTGATT+AGG + chr5_1:62026914-62026933 None:intergenic 15.0%
!!! AATCAAAGTTTATAAAGTTT+TGG - chr5_1:62026914-62026933 Msa0742210:intron 15.0%
!! TGTTTAATATATAAGAGAAT+TGG - chr5_1:62027477-62027496 Msa0742210:CDS 15.0%
!! ATTTGTGAGAAAAGAAAATT+TGG + chr5_1:62025678-62025697 None:intergenic 20.0%
!!! TTGAGTTTAAATGTCAATTT+TGG - chr5_1:62026239-62026258 Msa0742210:intron 20.0%
!!! AAGTACTTAGTTTTATTTGT+AGG - chr5_1:62026438-62026457 Msa0742210:intron 20.0%
!! GATTCTGTAACAAAAAAAAA+AGG - chr5_1:62026496-62026515 Msa0742210:intron 20.0%
!! AAAAAAAAGGGATTTATTGT+TGG - chr5_1:62026509-62026528 Msa0742210:intron 20.0%
!! AATGCATTAACTATTCATAA+AGG + chr5_1:62026575-62026594 None:intergenic 20.0%
!!! GATTTTTTCTTTTATTCTGA+AGG + chr5_1:62026667-62026686 None:intergenic 20.0%
!! TATAATACGTATATGTTAGA+TGG - chr5_1:62027393-62027412 Msa0742210:intron 20.0%
!! ATTTGTGAGAAAAGAAAATT+TGG + chr5_1:62025678-62025697 None:intergenic 20.0%
!!! TTGAGTTTAAATGTCAATTT+TGG - chr5_1:62026239-62026258 Msa0742210:intron 20.0%
!!! AAGTACTTAGTTTTATTTGT+AGG - chr5_1:62026438-62026457 Msa0742210:intron 20.0%
!! GATTCTGTAACAAAAAAAAA+AGG - chr5_1:62026496-62026515 Msa0742210:intron 20.0%
!! AAAAAAAAGGGATTTATTGT+TGG - chr5_1:62026509-62026528 Msa0742210:intron 20.0%
!! AATGCATTAACTATTCATAA+AGG + chr5_1:62026575-62026594 None:intergenic 20.0%
!!! GATTTTTTCTTTTATTCTGA+AGG + chr5_1:62026667-62026686 None:intergenic 20.0%
!! TATAATACGTATATGTTAGA+TGG - chr5_1:62027393-62027412 Msa0742210:intron 20.0%
! AATGTGTATACTTTACTTTG+AGG + chr5_1:62025591-62025610 None:intergenic 25.0%
!! AATTTGAAGTGAAGTGAATT+GGG + chr5_1:62025652-62025671 None:intergenic 25.0%
!!! TGATTTTGATTCGTATTTTG+TGG - chr5_1:62026015-62026034 Msa0742210:CDS 25.0%
! TGCACATTAAATAAAAATGC+AGG + chr5_1:62026128-62026147 None:intergenic 25.0%
!!! TCAAGTGATCTATTTTTGTT+TGG - chr5_1:62026182-62026201 Msa0742210:intron 25.0%
!! AGTTTAAATGTCAATTTTGG+AGG - chr5_1:62026242-62026261 Msa0742210:intron 25.0%
! CATAATCATTTCTATTCTAG+AGG - chr5_1:62026265-62026284 Msa0742210:intron 25.0%
! ATGAATAGTTAATGCATTCA+CGG - chr5_1:62026577-62026596 Msa0742210:intron 25.0%
!!! ACTATTTTTCTAGCTAATGA+AGG - chr5_1:62026622-62026641 Msa0742210:intron 25.0%
!!! TCTTTTATTCTGAAGGTAAA+TGG + chr5_1:62026660-62026679 None:intergenic 25.0%
! TTCAGATGTATTATTGTACT+TGG - chr5_1:62027416-62027435 Msa0742210:CDS 25.0%
! ATAGGTCAATACATTTCATT+TGG + chr5_1:62027636-62027655 None:intergenic 25.0%
!! AAATGTATTGACCTATTACT+TGG - chr5_1:62027640-62027659 Msa0742210:five_prime_UTR 25.0%
! TATGCCAGATTTCATATAAA+AGG + chr5_1:62027702-62027721 None:intergenic 25.0%
! AATGTGTATACTTTACTTTG+AGG + chr5_1:62025591-62025610 None:intergenic 25.0%
!! AATTTGAAGTGAAGTGAATT+GGG + chr5_1:62025652-62025671 None:intergenic 25.0%
!!! TGATTTTGATTCGTATTTTG+TGG - chr5_1:62026015-62026034 Msa0742210:CDS 25.0%
! TGCACATTAAATAAAAATGC+AGG + chr5_1:62026128-62026147 None:intergenic 25.0%
!!! TCAAGTGATCTATTTTTGTT+TGG - chr5_1:62026182-62026201 Msa0742210:intron 25.0%
!! AGTTTAAATGTCAATTTTGG+AGG - chr5_1:62026242-62026261 Msa0742210:intron 25.0%
! CATAATCATTTCTATTCTAG+AGG - chr5_1:62026265-62026284 Msa0742210:intron 25.0%
! ATGAATAGTTAATGCATTCA+CGG - chr5_1:62026577-62026596 Msa0742210:intron 25.0%
!!! ACTATTTTTCTAGCTAATGA+AGG - chr5_1:62026622-62026641 Msa0742210:intron 25.0%
!!! TCTTTTATTCTGAAGGTAAA+TGG + chr5_1:62026660-62026679 None:intergenic 25.0%
! TTCAGATGTATTATTGTACT+TGG - chr5_1:62027416-62027435 Msa0742210:CDS 25.0%
! ATAGGTCAATACATTTCATT+TGG + chr5_1:62027636-62027655 None:intergenic 25.0%
!! AAATGTATTGACCTATTACT+TGG - chr5_1:62027640-62027659 Msa0742210:five_prime_UTR 25.0%
! TATGCCAGATTTCATATAAA+AGG + chr5_1:62027702-62027721 None:intergenic 25.0%
! GAATTTGAAGTGAAGTGAAT+TGG + chr5_1:62025653-62025672 None:intergenic 30.0%
TTATTCGGTTTCATCCATAA+AGG - chr5_1:62025778-62025797 Msa0742210:three_prime_UTR 30.0%
! TGAAATGAAAAAAGCGCATA+CGG + chr5_1:62025924-62025943 None:intergenic 30.0%
ATTCATAAAGGACAAGTTCA+TGG + chr5_1:62026563-62026582 None:intergenic 30.0%
! TATTCTGAAGGTAAATGGTA+TGG + chr5_1:62026655-62026674 None:intergenic 30.0%
ACTTATTCTAATTGTGCTGT+TGG - chr5_1:62026848-62026867 Msa0742210:intron 30.0%
! CTTATTCTAATTGTGCTGTT+GGG - chr5_1:62026849-62026868 Msa0742210:intron 30.0%
AAAGAACTTGCACTAAACAA+GGG + chr5_1:62026981-62027000 None:intergenic 30.0%
AAAAGAACTTGCACTAAACA+AGG + chr5_1:62026982-62027001 None:intergenic 30.0%
!! TGTTTGTTTGCTAGTCTTAA+TGG - chr5_1:62027099-62027118 Msa0742210:intron 30.0%
TTGTAAGCATTATGCAAGTT+TGG - chr5_1:62027149-62027168 Msa0742210:intron 30.0%
!! GCAATGTATGTTTTTGTATC+TGG - chr5_1:62027235-62027254 Msa0742210:intron 30.0%
TTCATGAAGAAAAAGAGGAA+AGG + chr5_1:62027544-62027563 None:intergenic 30.0%
AATGCTTCATGAAGAAAAAG+AGG + chr5_1:62027549-62027568 None:intergenic 30.0%
! TCTTTTTCTTCATGAAGCAT+TGG - chr5_1:62027548-62027567 Msa0742210:CDS 30.0%
!! CTTTTTCTTCATGAAGCATT+GGG - chr5_1:62027549-62027568 Msa0742210:CDS 30.0%
!!! TTTTTCTTCATGAAGCATTG+GGG - chr5_1:62027550-62027569 Msa0742210:CDS 30.0%
! TGCACCTTTTATATGAAATC+TGG - chr5_1:62027695-62027714 Msa0742210:five_prime_UTR 30.0%
! GAATTTGAAGTGAAGTGAAT+TGG + chr5_1:62025653-62025672 None:intergenic 30.0%
TTATTCGGTTTCATCCATAA+AGG - chr5_1:62025778-62025797 Msa0742210:three_prime_UTR 30.0%
! TGAAATGAAAAAAGCGCATA+CGG + chr5_1:62025924-62025943 None:intergenic 30.0%
ATTCATAAAGGACAAGTTCA+TGG + chr5_1:62026563-62026582 None:intergenic 30.0%
! TATTCTGAAGGTAAATGGTA+TGG + chr5_1:62026655-62026674 None:intergenic 30.0%
ACTTATTCTAATTGTGCTGT+TGG - chr5_1:62026848-62026867 Msa0742210:intron 30.0%
! CTTATTCTAATTGTGCTGTT+GGG - chr5_1:62026849-62026868 Msa0742210:intron 30.0%
AAAGAACTTGCACTAAACAA+GGG + chr5_1:62026981-62027000 None:intergenic 30.0%
AAAAGAACTTGCACTAAACA+AGG + chr5_1:62026982-62027001 None:intergenic 30.0%
!! TGTTTGTTTGCTAGTCTTAA+TGG - chr5_1:62027099-62027118 Msa0742210:intron 30.0%
TTGTAAGCATTATGCAAGTT+TGG - chr5_1:62027149-62027168 Msa0742210:intron 30.0%
!! GCAATGTATGTTTTTGTATC+TGG - chr5_1:62027235-62027254 Msa0742210:intron 30.0%
TTCATGAAGAAAAAGAGGAA+AGG + chr5_1:62027544-62027563 None:intergenic 30.0%
AATGCTTCATGAAGAAAAAG+AGG + chr5_1:62027549-62027568 None:intergenic 30.0%
! TCTTTTTCTTCATGAAGCAT+TGG - chr5_1:62027548-62027567 Msa0742210:CDS 30.0%
!! CTTTTTCTTCATGAAGCATT+GGG - chr5_1:62027549-62027568 Msa0742210:CDS 30.0%
!!! TTTTTCTTCATGAAGCATTG+GGG - chr5_1:62027550-62027569 Msa0742210:CDS 30.0%
! TGCACCTTTTATATGAAATC+TGG - chr5_1:62027695-62027714 Msa0742210:five_prime_UTR 30.0%
AACGATGGAGAAAAAGTCTT+GGG + chr5_1:62025627-62025646 None:intergenic 35.0%
TTCTGTTTCACAATTCCGTA+CGG - chr5_1:62025733-62025752 Msa0742210:three_prime_UTR 35.0%
! AATTCCGTACGGATTGTTAT+TGG - chr5_1:62025744-62025763 Msa0742210:three_prime_UTR 35.0%
! TGCATTCACGGATGTATTTT+TGG - chr5_1:62026589-62026608 Msa0742210:intron 35.0%
! GACTTGAGTTTTTCTCCTAA+GGG + chr5_1:62026706-62026725 None:intergenic 35.0%
! AGACTTGAGTTTTTCTCCTA+AGG + chr5_1:62026707-62026726 None:intergenic 35.0%
GAAAAACTCAAGTCTCAAGT+TGG - chr5_1:62026712-62026731 Msa0742210:intron 35.0%
!!! ACGCCTTACAAGTTGATTTT+AGG - chr5_1:62026781-62026800 Msa0742210:intron 35.0%
!!! CGCCTTACAAGTTGATTTTA+GGG - chr5_1:62026782-62026801 Msa0742210:intron 35.0%
!!! GCCTTACAAGTTGATTTTAG+GGG - chr5_1:62026783-62026802 Msa0742210:intron 35.0%
ACCCCTAAAATCAACTTGTA+AGG + chr5_1:62026787-62026806 None:intergenic 35.0%
TTGTGTCGAATTGTAAAGTG+AGG + chr5_1:62026944-62026963 None:intergenic 35.0%
CAATGCACATTCATAAGTAG+CGG + chr5_1:62027196-62027215 None:intergenic 35.0%
! TTCCTTTTCTTTTTGTGCAG+TGG - chr5_1:62027271-62027290 Msa0742210:intron 35.0%
TATCTGTACAAACTAGCAAC+TGG - chr5_1:62027310-62027329 Msa0742210:intron 35.0%
! TACTTGGTGATTTCAGAGTT+TGG - chr5_1:62027432-62027451 Msa0742210:CDS 35.0%
! ATAAAAGGTGCAATCACAGA+AGG + chr5_1:62027687-62027706 None:intergenic 35.0%
AACGATGGAGAAAAAGTCTT+GGG + chr5_1:62025627-62025646 None:intergenic 35.0%
TTCTGTTTCACAATTCCGTA+CGG - chr5_1:62025733-62025752 Msa0742210:three_prime_UTR 35.0%
! AATTCCGTACGGATTGTTAT+TGG - chr5_1:62025744-62025763 Msa0742210:three_prime_UTR 35.0%
! TGCATTCACGGATGTATTTT+TGG - chr5_1:62026589-62026608 Msa0742210:intron 35.0%
! GACTTGAGTTTTTCTCCTAA+GGG + chr5_1:62026706-62026725 None:intergenic 35.0%
! AGACTTGAGTTTTTCTCCTA+AGG + chr5_1:62026707-62026726 None:intergenic 35.0%
GAAAAACTCAAGTCTCAAGT+TGG - chr5_1:62026712-62026731 Msa0742210:intron 35.0%
!!! ACGCCTTACAAGTTGATTTT+AGG - chr5_1:62026781-62026800 Msa0742210:intron 35.0%
!!! CGCCTTACAAGTTGATTTTA+GGG - chr5_1:62026782-62026801 Msa0742210:intron 35.0%
!!! GCCTTACAAGTTGATTTTAG+GGG - chr5_1:62026783-62026802 Msa0742210:intron 35.0%
ACCCCTAAAATCAACTTGTA+AGG + chr5_1:62026787-62026806 None:intergenic 35.0%
TTGTGTCGAATTGTAAAGTG+AGG + chr5_1:62026944-62026963 None:intergenic 35.0%
CAATGCACATTCATAAGTAG+CGG + chr5_1:62027196-62027215 None:intergenic 35.0%
! TTCCTTTTCTTTTTGTGCAG+TGG - chr5_1:62027271-62027290 Msa0742210:intron 35.0%
TATCTGTACAAACTAGCAAC+TGG - chr5_1:62027310-62027329 Msa0742210:intron 35.0%
! TACTTGGTGATTTCAGAGTT+TGG - chr5_1:62027432-62027451 Msa0742210:CDS 35.0%
! ATAAAAGGTGCAATCACAGA+AGG + chr5_1:62027687-62027706 None:intergenic 35.0%
!! ATACTTTACTTTGAGGCGTG+TGG + chr5_1:62025584-62025603 None:intergenic 40.0%
CAACGATGGAGAAAAAGTCT+TGG + chr5_1:62025628-62025647 None:intergenic 40.0%
TATCTCAGTCTCAATGCCTT+TGG - chr5_1:62025859-62025878 Msa0742210:three_prime_UTR 40.0%
ATCTCAGTCTCAATGCCTTT+GGG - chr5_1:62025860-62025879 Msa0742210:three_prime_UTR 40.0%
TGTTGCAAAACAGTTTGTGC+AGG - chr5_1:62026318-62026337 Msa0742210:intron 40.0%
TTTGTGCAGGTGTACTAACA+TGG - chr5_1:62026331-62026350 Msa0742210:intron 40.0%
AGGTGTACTAACATGGATCA+TGG - chr5_1:62026338-62026357 Msa0742210:intron 40.0%
GGTGTACTAACATGGATCAT+GGG - chr5_1:62026339-62026358 Msa0742210:intron 40.0%
! ACATCTAAGATTATGCCTGC+TGG - chr5_1:62026378-62026397 Msa0742210:intron 40.0%
GCAGGACTCAGGTAATTAAA+AGG - chr5_1:62026408-62026427 Msa0742210:intron 40.0%
AGTTTGCGATATCATCCCTT+AGG - chr5_1:62026688-62026707 Msa0742210:intron 40.0%
!! ACTCAAGTCTCAAGTTGGTT+AGG - chr5_1:62026717-62026736 Msa0742210:intron 40.0%
!! CTCAAGTCTCAAGTTGGTTA+GGG - chr5_1:62026718-62026737 Msa0742210:intron 40.0%
ATTAGGGCTATTGTGTTGTG+TGG + chr5_1:62026897-62026916 None:intergenic 40.0%
GACCACTGCACAAAAAGAAA+AGG + chr5_1:62027276-62027295 None:intergenic 40.0%
TCATATACCTGCTACTGCTA+AGG - chr5_1:62027339-62027358 Msa0742210:intron 40.0%
TATGCGTCTGTCTTGCATTT+AGG + chr5_1:62027580-62027599 None:intergenic 40.0%
TTGCACAGTAGCCAAGTAAT+AGG + chr5_1:62027654-62027673 None:intergenic 40.0%
!! ATACTTTACTTTGAGGCGTG+TGG + chr5_1:62025584-62025603 None:intergenic 40.0%
CAACGATGGAGAAAAAGTCT+TGG + chr5_1:62025628-62025647 None:intergenic 40.0%
TATCTCAGTCTCAATGCCTT+TGG - chr5_1:62025859-62025878 Msa0742210:three_prime_UTR 40.0%
ATCTCAGTCTCAATGCCTTT+GGG - chr5_1:62025860-62025879 Msa0742210:three_prime_UTR 40.0%
TGTTGCAAAACAGTTTGTGC+AGG - chr5_1:62026318-62026337 Msa0742210:intron 40.0%
TTTGTGCAGGTGTACTAACA+TGG - chr5_1:62026331-62026350 Msa0742210:intron 40.0%
AGGTGTACTAACATGGATCA+TGG - chr5_1:62026338-62026357 Msa0742210:intron 40.0%
GGTGTACTAACATGGATCAT+GGG - chr5_1:62026339-62026358 Msa0742210:intron 40.0%
! ACATCTAAGATTATGCCTGC+TGG - chr5_1:62026378-62026397 Msa0742210:intron 40.0%
GCAGGACTCAGGTAATTAAA+AGG - chr5_1:62026408-62026427 Msa0742210:intron 40.0%
AGTTTGCGATATCATCCCTT+AGG - chr5_1:62026688-62026707 Msa0742210:intron 40.0%
!! ACTCAAGTCTCAAGTTGGTT+AGG - chr5_1:62026717-62026736 Msa0742210:intron 40.0%
!! CTCAAGTCTCAAGTTGGTTA+GGG - chr5_1:62026718-62026737 Msa0742210:intron 40.0%
ATTAGGGCTATTGTGTTGTG+TGG + chr5_1:62026897-62026916 None:intergenic 40.0%
GACCACTGCACAAAAAGAAA+AGG + chr5_1:62027276-62027295 None:intergenic 40.0%
TCATATACCTGCTACTGCTA+AGG - chr5_1:62027339-62027358 Msa0742210:intron 40.0%
TATGCGTCTGTCTTGCATTT+AGG + chr5_1:62027580-62027599 None:intergenic 40.0%
TTGCACAGTAGCCAAGTAAT+AGG + chr5_1:62027654-62027673 None:intergenic 40.0%
GAAGTGAATTGGGACAACGA+TGG + chr5_1:62025642-62025661 None:intergenic 45.0%
CCGACCAATAACAATCCGTA+CGG + chr5_1:62025751-62025770 None:intergenic 45.0%
! CCGTACGGATTGTTATTGGT+CGG - chr5_1:62025748-62025767 Msa0742210:three_prime_UTR 45.0%
! TACGGATTGTTATTGGTCGG+TGG - chr5_1:62025751-62025770 Msa0742210:three_prime_UTR 45.0%
TTGGTCGGTGGAGGATTATT+CGG - chr5_1:62025763-62025782 Msa0742210:three_prime_UTR 45.0%
GAGAAGCGGTACTTCCTTTA+TGG + chr5_1:62025795-62025814 None:intergenic 45.0%
! GGCATTGAGACTGAGATAAC+CGG + chr5_1:62025857-62025876 None:intergenic 45.0%
! AGAGTTTTTCCGCTTCCCAA+AGG + chr5_1:62025878-62025897 None:intergenic 45.0%
AACTTGCACTAAACAAGGGC+AGG + chr5_1:62026977-62026996 None:intergenic 45.0%
CTGTACAAACTAGCAACTGG+TGG - chr5_1:62027313-62027332 Msa0742210:intron 45.0%
GACTGCATTGTTCTGTAAGC+AGG - chr5_1:62027504-62027523 Msa0742210:CDS 45.0%
GGTGCAATCACAGAAGGAAA+TGG + chr5_1:62027681-62027700 None:intergenic 45.0%
GAAGTGAATTGGGACAACGA+TGG + chr5_1:62025642-62025661 None:intergenic 45.0%
CCGACCAATAACAATCCGTA+CGG + chr5_1:62025751-62025770 None:intergenic 45.0%
! CCGTACGGATTGTTATTGGT+CGG - chr5_1:62025748-62025767 Msa0742210:three_prime_UTR 45.0%
! TACGGATTGTTATTGGTCGG+TGG - chr5_1:62025751-62025770 Msa0742210:three_prime_UTR 45.0%
TTGGTCGGTGGAGGATTATT+CGG - chr5_1:62025763-62025782 Msa0742210:three_prime_UTR 45.0%
GAGAAGCGGTACTTCCTTTA+TGG + chr5_1:62025795-62025814 None:intergenic 45.0%
! GGCATTGAGACTGAGATAAC+CGG + chr5_1:62025857-62025876 None:intergenic 45.0%
! AGAGTTTTTCCGCTTCCCAA+AGG + chr5_1:62025878-62025897 None:intergenic 45.0%
AACTTGCACTAAACAAGGGC+AGG + chr5_1:62026977-62026996 None:intergenic 45.0%
CTGTACAAACTAGCAACTGG+TGG - chr5_1:62027313-62027332 Msa0742210:intron 45.0%
GACTGCATTGTTCTGTAAGC+AGG - chr5_1:62027504-62027523 Msa0742210:CDS 45.0%
GGTGCAATCACAGAAGGAAA+TGG + chr5_1:62027681-62027700 None:intergenic 45.0%
! GGATTGTTATTGGTCGGTGG+AGG - chr5_1:62025754-62025773 Msa0742210:three_prime_UTR 50.0%
! GTCTCAATGCCTTTGGGAAG+CGG - chr5_1:62025866-62025885 Msa0742210:three_prime_UTR 50.0%
AAAGCGCATACGGATCTCGA+TGG + chr5_1:62025914-62025933 None:intergenic 50.0%
ATGCCTGCTGGTATGATTGC+AGG - chr5_1:62026390-62026409 Msa0742210:intron 50.0%
AGTCCTGCAATCATACCAGC+AGG + chr5_1:62026396-62026415 None:intergenic 50.0%
CTGGTATGATTGCAGGACTC+AGG - chr5_1:62026397-62026416 Msa0742210:intron 50.0%
! GGATTGTTATTGGTCGGTGG+AGG - chr5_1:62025754-62025773 Msa0742210:three_prime_UTR 50.0%
! GTCTCAATGCCTTTGGGAAG+CGG - chr5_1:62025866-62025885 Msa0742210:three_prime_UTR 50.0%
AAAGCGCATACGGATCTCGA+TGG + chr5_1:62025914-62025933 None:intergenic 50.0%
ATGCCTGCTGGTATGATTGC+AGG - chr5_1:62026390-62026409 Msa0742210:intron 50.0%
AGTCCTGCAATCATACCAGC+AGG + chr5_1:62026396-62026415 None:intergenic 50.0%
CTGGTATGATTGCAGGACTC+AGG - chr5_1:62026397-62026416 Msa0742210:intron 50.0%
TGAGACTGAGATAACCGGCG+AGG + chr5_1:62025852-62025871 None:intergenic 55.0%
CAGTCAGCCTTAGCAGTAGC+AGG + chr5_1:62027349-62027368 None:intergenic 55.0%
TGAGACTGAGATAACCGGCG+AGG + chr5_1:62025852-62025871 None:intergenic 55.0%
CAGTCAGCCTTAGCAGTAGC+AGG + chr5_1:62027349-62027368 None:intergenic 55.0%
!! GGAAGTACCGCTTCTCTCGC+CGG - chr5_1:62025799-62025818 Msa0742210:three_prime_UTR 60.0%
GGATCTCGATGGCGAGAGCA+AGG + chr5_1:62025903-62025922 None:intergenic 60.0%
!! GGAAGTACCGCTTCTCTCGC+CGG - chr5_1:62025799-62025818 Msa0742210:three_prime_UTR 60.0%
GGATCTCGATGGCGAGAGCA+AGG + chr5_1:62025903-62025922 None:intergenic 60.0%
AGTACCGCTTCTCTCGCCGG+CGG - chr5_1:62025802-62025821 Msa0742210:three_prime_UTR 65.0%
GGCGAGGATGAGAAGCGACC+CGG + chr5_1:62025836-62025855 None:intergenic 65.0%
GGGTCGCTTCTCATCCTCGC+CGG - chr5_1:62025835-62025854 Msa0742210:three_prime_UTR 65.0%
AGTACCGCTTCTCTCGCCGG+CGG - chr5_1:62025802-62025821 Msa0742210:three_prime_UTR 65.0%
GGCGAGGATGAGAAGCGACC+CGG + chr5_1:62025836-62025855 None:intergenic 65.0%
GGGTCGCTTCTCATCCTCGC+CGG - chr5_1:62025835-62025854 Msa0742210:three_prime_UTR 65.0%
GACGCCGCCGGCGAGAGAAG+CGG + chr5_1:62025809-62025828 None:intergenic 75.0%
GCTTCTCTCGCCGGCGGCGT+CGG - chr5_1:62025808-62025827 Msa0742210:three_prime_UTR 75.0%
GACGCCGCCGGCGAGAGAAG+CGG + chr5_1:62025809-62025828 None:intergenic 75.0%
GCTTCTCTCGCCGGCGGCGT+CGG - chr5_1:62025808-62025827 Msa0742210:three_prime_UTR 75.0%
!! CTCGCCGGCGGCGTCGGAAC+CGG - chr5_1:62025814-62025833 Msa0742210:three_prime_UTR 80.0%
!! TCGCCGGCGGCGTCGGAACC+GGG - chr5_1:62025815-62025834 Msa0742210:three_prime_UTR 80.0%
! CGACCCGGTTCCGACGCCGC+CGG + chr5_1:62025821-62025840 None:intergenic 80.0%
!! CTCGCCGGCGGCGTCGGAAC+CGG - chr5_1:62025814-62025833 Msa0742210:three_prime_UTR 80.0%
!! TCGCCGGCGGCGTCGGAACC+GGG - chr5_1:62025815-62025834 Msa0742210:three_prime_UTR 80.0%
! CGACCCGGTTCCGACGCCGC+CGG + chr5_1:62025821-62025840 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 62025574 62027759 62025574 ID=Msa0742210;Name=Msa0742210
chr5_1 mRNA 62025574 62027759 62025574 ID=Msa0742210-mRNA-1;Parent=Msa0742210;Name=Msa0742210-mRNA-1;_AED=0.00;_eAED=0.00;_QI=150|1|1|1|1|1|3|390|121
chr5_1 exon 62025574 62026042 62025574 ID=Msa0742210-mRNA-1:exon:18157;Parent=Msa0742210-mRNA-1
chr5_1 exon 62026915 62027002 62026915 ID=Msa0742210-mRNA-1:exon:18156;Parent=Msa0742210-mRNA-1
chr5_1 exon 62027411 62027759 62027411 ID=Msa0742210-mRNA-1:exon:18155;Parent=Msa0742210-mRNA-1
chr5_1 five_prime_UTR 62027610 62027759 62027610 ID=Msa0742210-mRNA-1:five_prime_utr;Parent=Msa0742210-mRNA-1
chr5_1 CDS 62027411 62027609 62027411 ID=Msa0742210-mRNA-1:cds;Parent=Msa0742210-mRNA-1
chr5_1 CDS 62026915 62027002 62026915 ID=Msa0742210-mRNA-1:cds;Parent=Msa0742210-mRNA-1
chr5_1 CDS 62025964 62026042 62025964 ID=Msa0742210-mRNA-1:cds;Parent=Msa0742210-mRNA-1
chr5_1 three_prime_UTR 62025574 62025963 62025574 ID=Msa0742210-mRNA-1:three_prime_utr;Parent=Msa0742210-mRNA-1
Gene Sequence

>Msa0742210

ATGCACGATTTCTGTTTCACAATTCCGTACGGATTGTTATTGGTCGGTGGAGGATTATTCGGTTTCATCCATAAAGGAAGTACCGCTTCTCTCGCCGGCGGCGTCGGAACCGGGTCGCTTCTCATCCTCGCCGGTTATCTCAGTCTCAATGCCTTTGGGAAGCGGAAAAACTCTTACCTTGCTCTCGCCATCGAGATCCTTTGTGCAGGTGTACTAACATGGATCATGGGACAACGTTACTTGCAAACATCTAAGATTATGCCTGCTGGTATGATTGCAGGACTCAGTGGTCTCATGACTCTGTTTTATCTGTACAAACTAGCAACTGGTGGCAATCATATACCTGCTACTGCTAAGGCTGACTGA
Protein sequence

>Msa0742210

MHDFCFTIPYGLLLVGGGLFGFIHKGSTASLAGGVGTGSLLILAGYLSLNAFGKRKNSYLALAIEILCAGVLTWIMGQRYLQTSKIMPAGMIAGLSGLMTLFYLYKLATGGNHIPATAKAD*