AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0891510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0891510 MtrunA17_Chr6g0472331 96.269 134 5 0 1 134 1 134 8.59e-95 269
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCGTGTTCTTCTCATTGT+TGG 0.219593 6_1:-66879472 None:intergenic
TTTGTCAAAGTCGCCATTAT+AGG 0.294994 6_1:-66880127 None:intergenic
CACAGCTATGGATTCTTCTA+TGG 0.298350 6_1:+66877608 None:intergenic
AAGAACGACAACCTTTGTTT+TGG 0.298817 6_1:-66877728 None:intergenic
TTGTCAAAGTCGCCATTATA+GGG 0.344757 6_1:-66880126 None:intergenic
AAAGTAAAACCACGTGGTTT+TGG 0.352984 6_1:+66877643 Msa0891510:CDS
CGTAAACTACCTCAATACTA+TGG 0.373289 6_1:+66877680 Msa0891510:CDS
CATGGTGAGGTTTCAAATCA+AGG 0.391023 6_1:-66877818 None:intergenic
GCCGTTCAATTCAAATGATC+AGG 0.427383 6_1:+66879507 Msa0891510:CDS
CTTCTTTGCAACAGATTCGA+TGG 0.428766 6_1:+66879447 Msa0891510:intron
AAAGGTGTAGCGCCCTATAA+TGG 0.435667 6_1:+66880114 Msa0891510:CDS
ATCTTATACCTGAAGGTGTA+TGG 0.459090 6_1:-66877860 None:intergenic
AGAAAACACCATACACCTTC+AGG 0.480517 6_1:+66877852 Msa0891510:CDS
AAGGGTTGATTCAGAGCTAT+CGG 0.489516 6_1:-66877799 None:intergenic
TTCTTTGCAACAGATTCGAT+GGG 0.490944 6_1:+66879448 Msa0891510:intron
AAGAATTTGACGGCTGTGAT+GGG 0.496731 6_1:-66877757 None:intergenic
GAAGAATTTGACGGCTGTGA+TGG 0.499319 6_1:-66877758 None:intergenic
TATAATGGCGACTTTGACAA+AGG 0.504926 6_1:+66880129 Msa0891510:CDS
ACCTGATCATTTGAATTGAA+CGG 0.508297 6_1:-66879508 None:intergenic
GATCGAGTCGAAGAATTTGA+CGG 0.532184 6_1:-66877767 None:intergenic
ATGGTGAGGTTTCAAATCAA+GGG 0.541262 6_1:-66877817 None:intergenic
AGGGTTGATTCAGAGCTATC+GGG 0.541321 6_1:-66877798 None:intergenic
CGTACAATGTTCCAAAACAA+AGG 0.556727 6_1:+66877717 Msa0891510:CDS
CTGGTGCGGCTTCCATGGTG+AGG 0.559379 6_1:-66877831 None:intergenic
AAGAAGCTTCCATAGTATTG+AGG 0.585611 6_1:-66877689 None:intergenic
CAAGTAGAAAATGAAAGCAA+AGG 0.600178 6_1:+66880096 Msa0891510:CDS
CTTGATTTGAAACCTCACCA+TGG 0.689590 6_1:+66877819 Msa0891510:CDS
TTCGTCTGTCCAAAACCACG+TGG 0.702042 6_1:-66877652 None:intergenic
GTCCAACAATGAGAAGAACA+CGG 0.724140 6_1:+66879470 Msa0891510:CDS
TACCAGAAAGTAAAACCACG+TGG 0.773547 6_1:+66877637 Msa0891510:CDS
CAACAATGAGAAGAACACGG+AGG 0.824433 6_1:+66879473 Msa0891510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATATTTATATTTTTTTAG+AGG + chr6_1:66878457-66878476 Msa0891510:intron 10.0%
!!! GATATTTATATTTTTTTAGA+GGG + chr6_1:66878458-66878477 Msa0891510:intron 10.0%
!!! TCGTATATAATTATATTTTT+TGG + chr6_1:66878739-66878758 Msa0891510:intron 10.0%
!!! TTTTTTTTTAAATAATGTAG+TGG - chr6_1:66879039-66879058 None:intergenic 10.0%
!! ATATAATAGACATTAAAAAA+AGG - chr6_1:66879330-66879349 None:intergenic 10.0%
!!! TTTTTTTAATGTCTATTATA+TGG + chr6_1:66879329-66879348 Msa0891510:intron 10.0%
!!! TTTTTTAATGTCTATTATAT+GGG + chr6_1:66879330-66879349 Msa0891510:intron 10.0%
!!! AATCTAAAATTAGATATTTT+TGG + chr6_1:66879969-66879988 Msa0891510:intron 10.0%
!!! TTATCGATGTTTTTTTTTTA+AGG - chr6_1:66877986-66878005 None:intergenic 15.0%
!! ATTTCGTTATTAAAGTTTAT+TGG + chr6_1:66878554-66878573 Msa0891510:intron 15.0%
!! TTTCGTTATTAAAGTTTATT+GGG + chr6_1:66878555-66878574 Msa0891510:intron 15.0%
!! TAATAAATTCATACTCTATT+AGG + chr6_1:66878866-66878885 Msa0891510:intron 15.0%
!!! TTTTTAATGTCTATTATATG+GGG + chr6_1:66879331-66879350 Msa0891510:intron 15.0%
!!! TCTATAGTCTTTTTTTTTTA+CGG - chr6_1:66879808-66879827 None:intergenic 15.0%
!!! TGGTTTGTGTATAAAATATA+AGG + chr6_1:66878130-66878149 Msa0891510:intron 20.0%
!! AGGTTAAAAAAAATTAGATG+AGG + chr6_1:66878150-66878169 Msa0891510:intron 20.0%
!! TATAATGGTAAACATGAAAA+AGG + chr6_1:66878832-66878851 Msa0891510:intron 20.0%
!! TTAGCAGTTATGTTAATTTA+TGG + chr6_1:66878934-66878953 Msa0891510:intron 20.0%
!! TAGCAGTTATGTTAATTTAT+GGG + chr6_1:66878935-66878954 Msa0891510:intron 20.0%
!! TATTATCACATCTACTAATA+AGG + chr6_1:66879120-66879139 Msa0891510:intron 20.0%
!! GTAGTATAGTATAAGATTAT+AGG + chr6_1:66879203-66879222 Msa0891510:intron 20.0%
!! AATGAAGAAAAATTCAAGTA+AGG + chr6_1:66879236-66879255 Msa0891510:intron 20.0%
!!! TTTTAATGTCTATTATATGG+GGG + chr6_1:66879332-66879351 Msa0891510:intron 20.0%
!! AACATTTATTGTCTTTAGTT+AGG + chr6_1:66879364-66879383 Msa0891510:intron 20.0%
!! ACATTTATTGTCTTTAGTTA+GGG + chr6_1:66879365-66879384 Msa0891510:intron 20.0%
!! AAAAAACTATACAATTGAGT+TGG - chr6_1:66879591-66879610 None:intergenic 20.0%
!! TAATGAAAAGTGAATTCATT+TGG + chr6_1:66879613-66879632 Msa0891510:intron 20.0%
!! GAAGAATAAATAGGAAATTT+AGG - chr6_1:66879697-66879716 None:intergenic 20.0%
!! CACATTTAAGAAGAATAAAT+AGG - chr6_1:66879706-66879725 None:intergenic 20.0%
!! TTTAAGTATATTAGTTCAGT+TGG + chr6_1:66879733-66879752 Msa0891510:intron 20.0%
!! GACAATACATAATATATGTA+AGG + chr6_1:66879760-66879779 Msa0891510:intron 20.0%
!! TACATAATATATGTAAGGTT+CGG + chr6_1:66879765-66879784 Msa0891510:intron 20.0%
! AAAAACAATCTTATACCTGA+AGG - chr6_1:66877870-66877889 None:intergenic 25.0%
!!! TTTATACACAAACCACTTTT+GGG - chr6_1:66878125-66878144 None:intergenic 25.0%
!! TTTTATACACAAACCACTTT+TGG - chr6_1:66878126-66878145 None:intergenic 25.0%
! GATGTTGTTAAGGTATAATT+AGG + chr6_1:66878172-66878191 Msa0891510:intron 25.0%
! TAACCTAATTGCTCAAAATT+AGG + chr6_1:66878214-66878233 Msa0891510:intron 25.0%
!! TAACCTAATTTTGAGCAATT+AGG - chr6_1:66878220-66878239 None:intergenic 25.0%
! AAATTCAACATGAACATGAA+GGG + chr6_1:66878520-66878539 Msa0891510:intron 25.0%
! TTACGAAGATATTATGTTAG+TGG - chr6_1:66878674-66878693 None:intergenic 25.0%
! TGTAATGAGTTAAAAACACT+TGG - chr6_1:66879156-66879175 None:intergenic 25.0%
! ACATAATATATGTAAGGTTC+GGG + chr6_1:66879766-66879785 Msa0891510:intron 25.0%
!!! ATAGTCTTTTTTTTTTACGG+TGG - chr6_1:66879805-66879824 None:intergenic 25.0%
! GTTCAATGTTTATAGTTTGA+TGG - chr6_1:66879938-66879957 None:intergenic 25.0%
!!! ATTTTTGGTGCTTTTCTTTT+TGG + chr6_1:66879984-66880003 Msa0891510:intron 25.0%
!!! TTTTGGTGATAGGATATTTT+TGG + chr6_1:66880001-66880020 Msa0891510:intron 25.0%
GACAATGCATTATATATGCA+AGG + chr6_1:66878073-66878092 Msa0891510:intron 30.0%
!! TTATACACAAACCACTTTTG+GGG - chr6_1:66878124-66878143 None:intergenic 30.0%
ATTAGATGAGGATGTTGTTA+AGG + chr6_1:66878162-66878181 Msa0891510:intron 30.0%
GAAATTCAACATGAACATGA+AGG + chr6_1:66878519-66878538 Msa0891510:intron 30.0%
ACATCGAAAGATGTTTAAGA+AGG - chr6_1:66878976-66878995 None:intergenic 30.0%
AATGTTCTATGTGGATAGTT+CGG - chr6_1:66879014-66879033 None:intergenic 30.0%
TAAGGAAGCAATGATTTATG+AGG + chr6_1:66879254-66879273 Msa0891510:intron 30.0%
CTCGAAAAATAAAGCATAGT+TGG - chr6_1:66879391-66879410 None:intergenic 30.0%
!!! CTATGCTTTATTTTTCGAGA+TGG + chr6_1:66879392-66879411 Msa0891510:intron 30.0%
!!! TTATTGACCACCGTATTTTT+TGG + chr6_1:66879421-66879440 Msa0891510:intron 30.0%
!!! TATTGACCACCGTATTTTTT+GGG + chr6_1:66879422-66879441 Msa0891510:intron 30.0%
AAAGAAGAGCCCAAAAAATA+CGG - chr6_1:66879434-66879453 None:intergenic 30.0%
ACCTGATCATTTGAATTGAA+CGG - chr6_1:66879511-66879530 None:intergenic 30.0%
TTATCACAAGACAAGAAGAA+AGG - chr6_1:66879561-66879580 None:intergenic 30.0%
! GTATATTAGTTCAGTTGGTA+AGG + chr6_1:66879738-66879757 Msa0891510:intron 30.0%
CATAATATATGTAAGGTTCG+GGG + chr6_1:66879767-66879786 Msa0891510:intron 30.0%
!!! CTTTTTTTTTTACGGTGGTT+GGG - chr6_1:66879800-66879819 None:intergenic 30.0%
!!! TCTTTTTTTTTTACGGTGGT+TGG - chr6_1:66879801-66879820 None:intergenic 30.0%
AAAAATGTAAACACATGCCT+TGG - chr6_1:66879901-66879920 None:intergenic 30.0%
! AAGGCATGTGTTTACATTTT+TGG + chr6_1:66879900-66879919 Msa0891510:intron 30.0%
!!! GATATTTTTGGTGTTTGTAG+AGG + chr6_1:66880013-66880032 Msa0891510:intron 30.0%
TGTAGAGGAGATAAATATAC+TGG + chr6_1:66880028-66880047 Msa0891510:intron 30.0%
TGGTCATAAAAGTGACTAAT+GGG + chr6_1:66880048-66880067 Msa0891510:intron 30.0%
AATGGGTGATTTATATGTGT+AGG + chr6_1:66880065-66880084 Msa0891510:intron 30.0%
! TGCTTTCATTTTCTACTTGT+GGG - chr6_1:66880095-66880114 None:intergenic 30.0%
! TTGCTTTCATTTTCTACTTG+TGG - chr6_1:66880096-66880115 None:intergenic 30.0%
CAAGTAGAAAATGAAAGCAA+AGG + chr6_1:66880096-66880115 Msa0891510:CDS 30.0%
! AAAGTAAAACCACGTGGTTT+TGG + chr6_1:66877643-66877662 Msa0891510:CDS 35.0%
CGTAAACTACCTCAATACTA+TGG + chr6_1:66877680-66877699 Msa0891510:CDS 35.0%
AAGAAGCTTCCATAGTATTG+AGG - chr6_1:66877692-66877711 None:intergenic 35.0%
CGTACAATGTTCCAAAACAA+AGG + chr6_1:66877717-66877736 Msa0891510:CDS 35.0%
!! AAGAACGACAACCTTTGTTT+TGG - chr6_1:66877731-66877750 None:intergenic 35.0%
ATGGTGAGGTTTCAAATCAA+GGG - chr6_1:66877820-66877839 None:intergenic 35.0%
! ATCTTATACCTGAAGGTGTA+TGG - chr6_1:66877863-66877882 None:intergenic 35.0%
ACACAAACCTCTAGTTAAGT+CGG - chr6_1:66878019-66878038 None:intergenic 35.0%
TGCATTATATATGCAAGGTC+CGG + chr6_1:66878078-66878097 Msa0891510:intron 35.0%
!! TTGTTATGCAAAGCGTTGAA+AGG + chr6_1:66878255-66878274 Msa0891510:intron 35.0%
! AGCGTTGAAAGGATTATTAC+TGG + chr6_1:66878266-66878285 Msa0891510:intron 35.0%
GTTGAAAGGATTATTACTGG+CGG + chr6_1:66878269-66878288 Msa0891510:intron 35.0%
!! ATTTACGTGCTGTTTGGTTT+AGG - chr6_1:66878309-66878328 None:intergenic 35.0%
! CATTCTATTTACGTGCTGTT+TGG - chr6_1:66878315-66878334 None:intergenic 35.0%
! GTAGTGGAAAATGTTCTATG+TGG - chr6_1:66879023-66879042 None:intergenic 35.0%
AAGCAATGATTTATGAGGAG+AGG + chr6_1:66879259-66879278 Msa0891510:intron 35.0%
AGCAATGATTTATGAGGAGA+GGG + chr6_1:66879260-66879279 Msa0891510:intron 35.0%
TTCTTTGCAACAGATTCGAT+GGG + chr6_1:66879448-66879467 Msa0891510:intron 35.0%
TTAGACTACCTAGAAAACCA+AGG + chr6_1:66879881-66879900 Msa0891510:intron 35.0%
!!! ATGTGTTTACATTTTTGGCG+TGG + chr6_1:66879905-66879924 Msa0891510:intron 35.0%
!! GTGCTTTTCTTTTTGGTGAT+AGG + chr6_1:66879991-66880010 Msa0891510:intron 35.0%
CTGGTCATAAAAGTGACTAA+TGG + chr6_1:66880047-66880066 Msa0891510:intron 35.0%
TTGTCAAAGTCGCCATTATA+GGG - chr6_1:66880129-66880148 None:intergenic 35.0%
TTTGTCAAAGTCGCCATTAT+AGG - chr6_1:66880130-66880149 None:intergenic 35.0%
TATAATGGCGACTTTGACAA+AGG + chr6_1:66880129-66880148 Msa0891510:CDS 35.0%
TACCAGAAAGTAAAACCACG+TGG + chr6_1:66877637-66877656 Msa0891510:CDS 40.0%
!! AACCACGTGGTTTTACTTTC+TGG - chr6_1:66877642-66877661 None:intergenic 40.0%
AAGAATTTGACGGCTGTGAT+GGG - chr6_1:66877760-66877779 None:intergenic 40.0%
GATCGAGTCGAAGAATTTGA+CGG - chr6_1:66877770-66877789 None:intergenic 40.0%
! AAGGGTTGATTCAGAGCTAT+CGG - chr6_1:66877802-66877821 None:intergenic 40.0%
CATGGTGAGGTTTCAAATCA+AGG - chr6_1:66877821-66877840 None:intergenic 40.0%
! CTTGATTTGAAACCTCACCA+TGG + chr6_1:66877819-66877838 Msa0891510:CDS 40.0%
!! TGAAGGTGTATGGTGTTTTC+TGG - chr6_1:66877853-66877872 None:intergenic 40.0%
AGAAAACACCATACACCTTC+AGG + chr6_1:66877852-66877871 Msa0891510:CDS 40.0%
GCATTATATATGCAAGGTCC+GGG + chr6_1:66878079-66878098 Msa0891510:intron 40.0%
CATTATATATGCAAGGTCCG+GGG + chr6_1:66878080-66878099 Msa0891510:intron 40.0%
GCAATGATTTATGAGGAGAG+GGG + chr6_1:66879261-66879280 Msa0891510:intron 40.0%
CAATGATTTATGAGGAGAGG+GGG + chr6_1:66879262-66879281 Msa0891510:intron 40.0%
CTTCTTTGCAACAGATTCGA+TGG + chr6_1:66879447-66879466 Msa0891510:intron 40.0%
GTCCAACAATGAGAAGAACA+CGG + chr6_1:66879470-66879489 Msa0891510:CDS 40.0%
GCCGTTCAATTCAAATGATC+AGG + chr6_1:66879507-66879526 Msa0891510:CDS 40.0%
! ATCAGGTAAGCTTTGTGTGT+TGG + chr6_1:66879524-66879543 Msa0891510:intron 40.0%
!! AACACATGCCTTGGTTTTCT+AGG - chr6_1:66879892-66879911 None:intergenic 40.0%
GGGTGATTTATATGTGTAGG+TGG + chr6_1:66880068-66880087 Msa0891510:intron 40.0%
GAAGAATTTGACGGCTGTGA+TGG - chr6_1:66877761-66877780 None:intergenic 45.0%
! AGGGTTGATTCAGAGCTATC+GGG - chr6_1:66877801-66877820 None:intergenic 45.0%
!! GTGTATGGTGTTTTCTGGTG+CGG - chr6_1:66877848-66877867 None:intergenic 45.0%
ATCTCCTCCGACTTAACTAG+AGG + chr6_1:66878009-66878028 Msa0891510:intron 45.0%
CAAACCTCTAGTTAAGTCGG+AGG - chr6_1:66878016-66878035 None:intergenic 45.0%
TGAGCTTAGCTCAACTGGTA+AGG + chr6_1:66878051-66878070 Msa0891510:intron 45.0%
!!! ACAAACCACTTTTGGGGTAG+TGG - chr6_1:66878118-66878137 None:intergenic 45.0%
GAAAGGATTATTACTGGCGG+TGG + chr6_1:66878272-66878291 Msa0891510:intron 45.0%
! TGTGGATAGTTCGGTTGATG+AGG - chr6_1:66879005-66879024 None:intergenic 45.0%
GAAGAGCCCAAAAAATACGG+TGG - chr6_1:66879431-66879450 None:intergenic 45.0%
CTCCGTGTTCTTCTCATTGT+TGG - chr6_1:66879475-66879494 None:intergenic 45.0%
CAACAATGAGAAGAACACGG+AGG + chr6_1:66879473-66879492 Msa0891510:CDS 45.0%
! AAAGGTGTAGCGCCCTATAA+TGG + chr6_1:66880114-66880133 Msa0891510:CDS 45.0%
!! TTAATAATAATTTGATATAA+TGG + chr6_1:66878817-66878836 Msa0891510:intron 5.0%
!!! TTAAATTACTTATTTATTTT+AGG + chr6_1:66878896-66878915 Msa0891510:intron 5.0%
!!! TAAATTACTTATTTATTTTA+GGG + chr6_1:66878897-66878916 Msa0891510:intron 5.0%
TTCGTCTGTCCAAAACCACG+TGG - chr6_1:66877655-66877674 None:intergenic 50.0%
TCTCGTGAGCTTAGCTCAAC+TGG + chr6_1:66878046-66878065 Msa0891510:intron 50.0%
GGATTATTACTGGCGGTGGT+TGG + chr6_1:66878276-66878295 Msa0891510:intron 50.0%
!! GTTTTCTGGTGCGGCTTCCA+TGG - chr6_1:66877839-66877858 None:intergenic 55.0%
!!! CACTTTTGGGGTAGTGGCTG+GGG - chr6_1:66878112-66878131 None:intergenic 55.0%
!!! CCACTTTTGGGGTAGTGGCT+GGG - chr6_1:66878113-66878132 None:intergenic 55.0%
!!! ACCACTTTTGGGGTAGTGGC+TGG - chr6_1:66878114-66878133 None:intergenic 55.0%
AGTGGCTGGGGTTTGAACCC+CGG - chr6_1:66878100-66878119 None:intergenic 60.0%
CCCAGCCACTACCCCAAAAG+TGG + chr6_1:66878110-66878129 Msa0891510:intron 60.0%
CTGGTGCGGCTTCCATGGTG+AGG - chr6_1:66877834-66877853 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr6_1 gene 66877615 66880164 66877615 ID=Msa0891510;Name=Msa0891510
chr6_1 mRNA 66877615 66880164 66877615 ID=Msa0891510-mRNA-1;Parent=Msa0891510;Name=Msa0891510-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|0|0|1|1|1|3|0|134
chr6_1 exon 66877615 66877873 66877615 ID=Msa0891510-mRNA-1:exon:11929;Parent=Msa0891510-mRNA-1
chr6_1 exon 66879461 66879528 66879461 ID=Msa0891510-mRNA-1:exon:11930;Parent=Msa0891510-mRNA-1
chr6_1 exon 66880087 66880164 66880087 ID=Msa0891510-mRNA-1:exon:11931;Parent=Msa0891510-mRNA-1
chr6_1 CDS 66877615 66877873 66877615 ID=Msa0891510-mRNA-1:cds;Parent=Msa0891510-mRNA-1
chr6_1 CDS 66879461 66879528 66879461 ID=Msa0891510-mRNA-1:cds;Parent=Msa0891510-mRNA-1
chr6_1 CDS 66880087 66880164 66880087 ID=Msa0891510-mRNA-1:cds;Parent=Msa0891510-mRNA-1
Gene Sequence

>Msa0891510

ATGGATTCTTCTATGGTGCAAGTACCAGAAAGTAAAACCACGTGGTTTTGGACAGACGAAAAACACGTAAACTACCTCAATACTATGGAAGCTTCTTTCGTTCGTACAATGTTCCAAAACAAAGGTTGTCGTTCTTCTTCTTCCCATCACAGCCGTCAAATTCTTCGACTCGATCGACAACTTCCCGATAGCTCTGAATCAACCCTTGATTTGAAACCTCACCATGGAAGCCGCACCAGAAAACACCATACACCTTCAGATTCGATGGGTCCAACAATGAGAAGAACACGGAGGAGATCATCTCAGCCGTTCAATTCAAATGATCAGGTGGTCCCACAAGTAGAAAATGAAAGCAAAGGTGTAGCGCCCTATAATGGCGACTTTGACAAAGGAGCAGAAAATTAA

Protein sequence

>Msa0891510

MDSSMVQVPESKTTWFWTDEKHVNYLNTMEASFVRTMFQNKGCRSSSSHHSRQILRLDRQLPDSSESTLDLKPHHGSRTRKHHTPSDSMGPTMRRTRRRSSQPFNSNDQVVPQVENESKGVAPYNGDFDKGAEN*