AlfalfaGEDB | Msa0936540

M. sativa cultivar zhongmu-4 / Msa0936540

KEGG: K01772|K08900
GO: GO:0000003 GO:0000302 GO:0000304 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005783 GO:0005911 GO:0006810 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0007584 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010154 GO:0010431 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0021700 GO:0022414 GO:0022607 GO:0022857 GO:0022884 GO:0030054 GO:0031667 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033993 GO:0034622 GO:0042221 GO:0042493 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051131 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0061458 GO:0065003 GO:0071695 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097305 GO:1901700 GO:1904680
PFAM: PF14363.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936540	XP_003618439.2	76.440	191	18	1	1	164	1	191	1.35e-91	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936540	sp\|Q9FLD5\|ASD_ARATH	42.857	175	72	2	3	150	4	177	1.86e-44	157

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0870840	Msa0936540	0.834487	2.913909e-56	-8.615850e-47
Msa0904010	Msa0936540	0.826234	3.033695e-54	-8.615850e-47
Msa0936540	Msa0936560	0.906098	2.131984e-80	-8.615850e-47
Msa0936540	Msa0973530	0.819441	1.159478e-52	-8.615850e-47
Msa0936540	Msa0973540	0.842435	2.590757e-58	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936540	MtrunA17_Chr6g0452391	76.440	191	18	1	1	164	1	191	1.24e-95	287
Msa0936540	MtrunA17_Chr6g0452371	64.171	187	39	2	1	159	3	189	2.65e-77	240
Msa0936540	MtrunA17_Chr6g0452451	61.582	177	38	3	1	149	3	177	1.25e-62	202
Msa0936540	MtrunA17_Chr7g0215951	52.299	174	54	2	3	149	1	172	5.78e-55	181
Msa0936540	MtrunA17_Chr6g0452401	70.690	116	34	0	34	149	39	154	6.98e-51	170
Msa0936540	MtrunA17_Chr6g0466811	45.128	195	66	5	10	173	21	205	4.92e-43	150
Msa0936540	MtrunA17_Chr1g0155131	40.341	176	77	2	1	149	1	175	4.78e-42	147
Msa0936540	MtrunA17_Chr2g0292561	40.116	172	75	2	5	149	8	178	7.58e-41	143
Msa0936540	MtrunA17_Chr4g0059811	41.279	172	72	4	5	149	8	177	2.63e-38	137
Msa0936540	MtrunA17_Chr1g0155121	37.209	172	77	3	5	149	3	170	4.64e-31	117
Msa0936540	MtrunA17_Chr6g0452351	45.000	120	38	1	10	101	21	140	6.01e-31	110
Msa0936540	MtrunA17_Chr6g0452411	66.216	74	25	0	76	149	1	74	4.71e-27	105
Msa0936540	MtrunA17_Chr4g0072491	28.655	171	96	2	5	150	3	172	1.16e-16	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936540	AT5G40010.1	42.857	175	72	2	3	150	4	177	1.89e-45	157
Msa0936540	AT3G28580.2	41.379	174	75	3	3	150	4	176	1.99e-43	150
Msa0936540	AT3G28580.1	41.379	174	75	3	3	150	4	176	2.89e-43	150
Msa0936540	AT3G28540.2	38.857	175	73	3	4	150	5	173	7.01e-31	117
Msa0936540	AT3G28540.1	38.857	175	73	3	4	150	5	173	7.56e-31	117
Msa0936540	AT3G28510.1	37.931	174	80	2	4	150	5	177	7.60e-31	117
Msa0936540	AT3G28560.1	40.476	168	78	4	4	150	5	171	6.47e-30	110
Msa0936540	AT3G28520.1	36.905	168	69	3	14	150	1	162	2.66e-22	93.6
Msa0936540	AT5G57480.1	28.070	171	97	2	5	150	3	172	6.73e-18	81.3
Msa0936540	AT3G28610.1	26.923	208	93	6	11	178	7	195	1.71e-17	79.7
Msa0936540	AT5G40000.1	27.128	188	84	5	1	149	2	175	4.68e-17	78.6
Msa0936540	AT3G28600.1	27.604	192	85	5	8	159	5	182	1.29e-16	77.4
Msa0936540	AT4G25835.1	26.901	171	99	2	5	150	3	172	8.39e-16	75.1
Msa0936540	AT4G30250.2	25.731	171	93	3	1	138	1	170	7.18e-14	69.3
Msa0936540	AT4G30250.1	25.444	169	92	3	3	138	1	168	2.21e-13	68.2
Msa0936540	AT3G28570.1	22.222	180	85	4	8	149	7	169	8.33e-12	63.5
Msa0936540	AT3G28570.2	22.222	180	85	4	8	149	14	176	8.71e-12	63.5

Find 26 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATGAAGAATAGGGATTT+TGG	0.146272	6_3:+7354303	Msa0936540:CDS
TGCTTAGAACAAGTGGATTT+TGG	0.205867	6_3:-7354074	None:intergenic
CTCTTACATCCAACATGTTT+TGG	0.206662	6_3:+7354263	Msa0936540:CDS
AGGCAATAACAATGAAGAAT+AGG	0.370355	6_3:+7354295	Msa0936540:CDS
GTCAAACCTAGGCTCAATAA+TGG	0.388651	6_3:+7353808	Msa0936540:CDS
GGCAATAACAATGAAGAATA+GGG	0.397758	6_3:+7354296	Msa0936540:CDS
TGAACTCATCTGTGATCTCT+TGG	0.398036	6_3:-7354107	None:intergenic
AGATGGGAATTGGTGAAATA+TGG	0.401989	6_3:+7353786	None:intergenic
ATATCATAAGAGATGGGAAT+TGG	0.405499	6_3:+7353776	None:intergenic
AGTTAGCACTAAGATATGTT+TGG	0.410357	6_3:-7354005	None:intergenic
AGTTCAATGGTGTCAAAGTT+TGG	0.425897	6_3:+7354124	Msa0936540:CDS
TTAACAAATCTACGAAGTGC+TGG	0.478784	6_3:-7353875	None:intergenic
TCATCCATGCTTAGAACAAG+TGG	0.497876	6_3:-7354081	None:intergenic
CTAAAATCTCTCATTGATGA+AGG	0.502280	6_3:-7354339	None:intergenic
CAAAACATGTTGGATGTAAG+AGG	0.509559	6_3:-7354262	None:intergenic
TTCCTTGTTCCAAAACATGT+TGG	0.518629	6_3:-7354272	None:intergenic
TGAAAATGATTGTGTTCTTG+AGG	0.555571	6_3:-7354166	None:intergenic
AGCACTAAGATATGTTTGGA+TGG	0.566183	6_3:-7354001	None:intergenic
ACAAATCTACGAAGTGCTGG+TGG	0.569895	6_3:-7353872	None:intergenic
TGGGAAGAATTTCTCATACA+TGG	0.570313	6_3:-7353852	None:intergenic
AAATCCACTTGTTCTAAGCA+TGG	0.575420	6_3:+7354077	Msa0936540:CDS
GAGATCACAGATGAGTTCAA+TGG	0.581658	6_3:+7354111	Msa0936540:CDS
TGCTAGCCATTATTGAGCCT+AGG	0.582090	6_3:-7353814	None:intergenic
CAAATCTACGAAGTGCTGGT+GGG	0.606728	6_3:-7353871	None:intergenic
GGTGAAATATGGTCAAACCT+AGG	0.619766	6_3:+7353797	Msa0936540:CDS
TCAATGGTGTCAAAGTTTGG+TGG	0.621281	6_3:+7354127	Msa0936540:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAATTTAGTGTTGTTGA+TGG	+	chr6_3:7354398-7354417	Msa0936540:CDS	20.0%
!	TATTCTCATAGAAGGTTATT+TGG	-	chr6_3:7353943-7353962	None:intergenic	25.0%
!!	TTCATCAATGAGAGATTTTA+GGG	+	chr6_3:7354341-7354360	Msa0936540:CDS	25.0%
	AGAAGGTTATTTGGATGTAA+GGG	-	chr6_3:7353934-7353953	None:intergenic	30.0%
	TAGAAGGTTATTTGGATGTA+AGG	-	chr6_3:7353935-7353954	None:intergenic	30.0%
	AACCTTCTATGAGAATAATC+AGG	+	chr6_3:7353946-7353965	Msa0936540:intron	30.0%
	AGTTAGCACTAAGATATGTT+TGG	-	chr6_3:7354008-7354027	None:intergenic	30.0%
	TGAAAATGATTGTGTTCTTG+AGG	-	chr6_3:7354169-7354188	None:intergenic	30.0%
!	ATCTTTTCTCATCTGACTTA+GGG	-	chr6_3:7354197-7354216	None:intergenic	30.0%
!	AATCTTTTCTCATCTGACTT+AGG	-	chr6_3:7354198-7354217	None:intergenic	30.0%
!	ATGTCTTTTATGGAAGGTTA+GGG	-	chr6_3:7354226-7354245	None:intergenic	30.0%
	AGGCAATAACAATGAAGAAT+AGG	+	chr6_3:7354295-7354314	Msa0936540:CDS	30.0%
	GGCAATAACAATGAAGAATA+GGG	+	chr6_3:7354296-7354315	Msa0936540:CDS	30.0%
!	ACAATGAAGAATAGGGATTT+TGG	+	chr6_3:7354303-7354322	Msa0936540:CDS	30.0%
!	CAATGAAGAATAGGGATTTT+GGG	+	chr6_3:7354304-7354323	Msa0936540:CDS	30.0%
!	AATGAAGAATAGGGATTTTG+GGG	+	chr6_3:7354305-7354324	Msa0936540:CDS	30.0%
	CTAAAATCTCTCATTGATGA+AGG	-	chr6_3:7354342-7354361	None:intergenic	30.0%
!	CTTCATCAATGAGAGATTTT+AGG	+	chr6_3:7354340-7354359	Msa0936540:CDS	30.0%
!!	ATGAGAGATTTTAGGGATTT+TGG	+	chr6_3:7354348-7354367	Msa0936540:CDS	30.0%
	TGGGAAGAATTTCTCATACA+TGG	-	chr6_3:7353855-7353874	None:intergenic	35.0%
	TTAACAAATCTACGAAGTGC+TGG	-	chr6_3:7353878-7353897	None:intergenic	35.0%
	CTCCTGATTATTCTCATAGA+AGG	-	chr6_3:7353951-7353970	None:intergenic	35.0%
	AGCACTAAGATATGTTTGGA+TGG	-	chr6_3:7354004-7354023	None:intergenic	35.0%
!!	TGCTTAGAACAAGTGGATTT+TGG	-	chr6_3:7354077-7354096	None:intergenic	35.0%
!	AAATCCACTTGTTCTAAGCA+TGG	+	chr6_3:7354077-7354096	Msa0936540:CDS	35.0%
!	AGTTCAATGGTGTCAAAGTT+TGG	+	chr6_3:7354124-7354143	Msa0936540:CDS	35.0%
!	GATGTCTTTTATGGAAGGTT+AGG	-	chr6_3:7354227-7354246	None:intergenic	35.0%
!	ATCACGATGTCTTTTATGGA+AGG	-	chr6_3:7354232-7354251	None:intergenic	35.0%
!	TGAGATCACGATGTCTTTTA+TGG	-	chr6_3:7354236-7354255	None:intergenic	35.0%
	CAAAACATGTTGGATGTAAG+AGG	-	chr6_3:7354265-7354284	None:intergenic	35.0%
!	CTCTTACATCCAACATGTTT+TGG	+	chr6_3:7354263-7354282	Msa0936540:CDS	35.0%
!!!	ATCCAACATGTTTTGGAACA+AGG	+	chr6_3:7354270-7354289	Msa0936540:CDS	35.0%
	TTCCTTGTTCCAAAACATGT+TGG	-	chr6_3:7354275-7354294	None:intergenic	35.0%
!!!	ACATGTTTTGGAACAAGGAA+AGG	+	chr6_3:7354275-7354294	Msa0936540:CDS	35.0%
!!	AATAGGGATTTTGGGGATTT+TGG	+	chr6_3:7354312-7354331	Msa0936540:CDS	35.0%
	GGTGAAATATGGTCAAACCT+AGG	+	chr6_3:7353797-7353816	Msa0936540:CDS	40.0%
	GTCAAACCTAGGCTCAATAA+TGG	+	chr6_3:7353808-7353827	Msa0936540:CDS	40.0%
	TCATCCATGCTTAGAACAAG+TGG	-	chr6_3:7354084-7354103	None:intergenic	40.0%
	TGAACTCATCTGTGATCTCT+TGG	-	chr6_3:7354110-7354129	None:intergenic	40.0%
	GAGATCACAGATGAGTTCAA+TGG	+	chr6_3:7354111-7354130	Msa0936540:CDS	40.0%
!	TCAATGGTGTCAAAGTTTGG+TGG	+	chr6_3:7354127-7354146	Msa0936540:CDS	40.0%
	TGCTAGCCATTATTGAGCCT+AGG	-	chr6_3:7353817-7353836	None:intergenic	45.0%
!!	CAAATCTACGAAGTGCTGGT+GGG	-	chr6_3:7353874-7353893	None:intergenic	45.0%
!!	ACAAATCTACGAAGTGCTGG+TGG	-	chr6_3:7353875-7353894	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	7353788	7354443	7353788	ID=Msa0936540;Name=Msa0936540
chr6_3	mRNA	7353788	7354443	7353788	ID=Msa0936540-mRNA-1;Parent=Msa0936540;Name=Msa0936540-mRNA-1;_AED=0.44;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|2\|0\|190
chr6_3	exon	7353788	7353884	7353788	ID=Msa0936540-mRNA-1:exon:2384;Parent=Msa0936540-mRNA-1
chr6_3	exon	7353968	7354443	7353968	ID=Msa0936540-mRNA-1:exon:2385;Parent=Msa0936540-mRNA-1
chr6_3	CDS	7353788	7353884	7353788	ID=Msa0936540-mRNA-1:cds;Parent=Msa0936540-mRNA-1
chr6_3	CDS	7353968	7354443	7353968	ID=Msa0936540-mRNA-1:cds;Parent=Msa0936540-mRNA-1

Gene Sequence

>Msa0936540

ATGGGAATTGGTGAAATATGGTCAAACCTAGGCTCAATAATGGCTAGCATAATGTTTGTATATGCCATGTATGAGAAATTCTTCCCACCAGCACTTCGAGATCATCTCAAACACAGCAAAACTTACACAACCATCCAAACATATCTTAGTGCTAACTCTTCAAAAAGAGCTAGAAGACTTAAAGCTGAAGTTATCAAAGATAGCCAAAATCCACTTGTTCTAAGCATGGATGACAACCAAGAGATCACAGATGAGTTCAATGGTGTCAAAGTTTGGTGGTCAGCAAATCATATCACCTCAAGAACACAATCATTTTCAATCTACCCTAAGTCAGATGAGAAAAGATTCTTAACCCTAACCTTCCATAAAAGACATCGTGATCTCATCACTACCTCTTACATCCAACATGTTTTGGAACAAGGAAAGGCAATAACAATGAAGAATAGGGATTTTGGGGATTTTGGATCTCCTTCATCAATGAGAGATTTTAGGGATTTTGGATCTGATTGCTTAAGTTTCTTCATTTTTTTTAATTTAGTGTTGTTGATGGTGATAATGATGATGAGATTGTGA

Protein sequence

>Msa0936540

MGIGEIWSNLGSIMASIMFVYAMYEKFFPPALRDHLKHSKTYTTIQTYLSANSSKRARRLKAEVIKDSQNPLVLSMDDNQEITDEFNGVKVWWSANHITSRTQSFSIYPKSDEKRFLTLTFHKRHRDLITTSYIQHVLEQGKAITMKNRDFGDFGSPSSMRDFRDFGSDCLSFFIFFNLVLLMVIMMMRL*