AlfalfaGEDB | Msa0973530

M. sativa cultivar zhongmu-4 / Msa0973530

KEGG: K01772|K08900
GO: GO:0000003 GO:0000302 GO:0000304 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005783 GO:0005911 GO:0006810 GO:0006950 GO:0006970 GO:0006979 GO:0007275 GO:0007584 GO:0008104 GO:0008150 GO:0008320 GO:0008565 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009506 GO:0009605 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010154 GO:0010431 GO:0012505 GO:0015031 GO:0015833 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0021700 GO:0022414 GO:0022607 GO:0022857 GO:0022884 GO:0030054 GO:0031667 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0033993 GO:0034622 GO:0042221 GO:0042493 GO:0042886 GO:0042887 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051131 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0061458 GO:0065003 GO:0071695 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0097305 GO:1901700 GO:1904680
PFAM: PF14363.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973530	XP_003618439.2	91.515	165	14	0	1	165	14	178	3.34e-105	322

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973530	sp\|Q9FLD5\|ASD_ARATH	47.561	164	85	1	1	164	15	177	1.42e-49	171

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973530	G7KK18	91.515	165	14	0	1	165	14	178	1.59e-105	322

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0091930	Msa0973530	0.820337	7.235531e-53	-8.615850e-47
Msa0708310	Msa0973530	0.810949	8.938392e-51	-8.615850e-47
Msa0780350	Msa0973530	0.822284	2.571693e-53	-8.615850e-47
Msa0834030	Msa0973530	0.811892	5.575896e-51	-8.615850e-47
Msa0243480	Msa0973530	0.816037	6.799093e-52	-8.615850e-47
Msa0321080	Msa0973530	0.823431	1.390302e-53	-8.615850e-47
Msa0870840	Msa0973530	0.818739	1.674946e-52	-8.615850e-47
Msa0904010	Msa0973530	0.838202	3.308689e-57	-8.615850e-47
Msa0936530	Msa0973530	0.839639	1.404996e-57	-8.615850e-47
Msa0936540	Msa0973530	0.819441	1.159478e-52	-8.615850e-47
Msa0936550	Msa0973530	0.830281	3.208728e-55	-8.615850e-47
Msa0936560	Msa0973530	0.881165	3.011184e-70	-8.615850e-47
Msa0964490	Msa0973530	0.817232	3.670062e-52	-8.615850e-47
Msa0973520	Msa0973530	0.837437	5.201029e-57	-8.615850e-47
Msa0973530	Msa0973540	0.860394	2.129205e-63	-8.615850e-47
Msa0973530	Msa1246700	0.801525	8.641040e-49	-8.615850e-47
Msa0973530	Msa1376450	0.801447	8.965890e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973530	MtrunA17_Chr6g0452391	91.515	165	14	0	1	165	14	178	3.07e-109	322
Msa0973530	MtrunA17_Chr6g0452371	70.455	176	50	1	1	176	16	189	3.01e-85	261
Msa0973530	MtrunA17_Chr6g0452451	67.630	173	53	2	2	173	17	187	4.03e-76	238
Msa0973530	MtrunA17_Chr6g0452401	67.532	154	50	0	10	163	1	154	2.49e-71	224
Msa0973530	MtrunA17_Chr7g0215951	58.896	163	65	1	1	163	12	172	7.07e-62	200
Msa0973530	MtrunA17_Chr6g0466811	57.927	164	60	2	1	163	25	180	7.65e-57	187
Msa0973530	MtrunA17_Chr1g0155131	46.821	173	91	1	1	173	14	185	1.19e-53	178
Msa0973530	MtrunA17_Chr2g0292561	45.087	173	94	1	1	173	17	188	2.70e-53	177
Msa0973530	MtrunA17_Chr4g0059811	47.239	163	84	2	1	163	17	177	1.79e-47	162
Msa0973530	MtrunA17_Chr1g0155121	41.718	163	91	2	1	163	12	170	5.88e-42	147
Msa0973530	MtrunA17_Chr6g0452351	53.448	116	53	1	1	115	25	140	5.93e-42	138
Msa0973530	MtrunA17_Chr6g0452411	67.568	74	24	0	90	163	1	74	3.47e-28	109
Msa0973530	MtrunA17_Chr4g0072491	31.765	170	105	4	1	164	8	172	3.63e-17	79.3
Msa0973530	MtrunA17_Chr2g0315971	29.012	162	108	3	1	158	11	169	2.65e-13	68.2
Msa0973530	MtrunA17_Chr2g0316021	28.313	166	112	4	1	162	11	173	3.34e-13	67.4
Msa0973530	MtrunA17_Chr5g0404651	23.121	173	115	5	2	163	22	187	5.25e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0973530	AT5G40010.1	47.561	164	85	1	1	164	15	177	1.45e-50	171
Msa0973530	AT3G28580.2	47.305	167	80	3	1	164	15	176	6.50e-49	166
Msa0973530	AT3G28580.1	47.305	167	80	3	1	164	15	176	8.15e-49	166
Msa0973530	AT3G28510.1	42.073	164	94	1	1	164	15	177	1.17e-37	137
Msa0973530	AT3G28540.2	41.818	165	89	3	1	164	15	173	4.17e-34	127
Msa0973530	AT3G28540.1	41.818	165	89	3	1	164	15	173	4.52e-34	127
Msa0973530	AT3G28560.1	41.212	165	88	3	1	164	15	171	5.76e-34	122
Msa0973530	AT3G28520.1	39.881	168	91	3	1	164	1	162	3.54e-29	113
Msa0973530	AT3G28610.1	31.579	209	106	7	1	195	10	195	4.58e-26	104
Msa0973530	AT3G28600.1	31.579	190	98	6	1	176	11	182	1.34e-23	97.8
Msa0973530	AT5G40000.1	30.508	177	93	5	1	163	15	175	7.89e-22	92.8
Msa0973530	AT5G57480.1	29.762	168	107	4	1	164	12	172	2.82e-17	79.7
Msa0973530	AT3G28570.1	25.568	176	99	5	1	163	13	169	6.68e-16	75.9
Msa0973530	AT3G28570.2	25.568	176	99	5	1	163	20	176	6.83e-16	75.9
Msa0973530	AT4G25835.1	28.313	166	112	3	1	164	12	172	4.48e-15	73.6
Msa0973530	AT4G30250.2	28.571	168	97	5	1	152	10	170	6.59e-13	67.0
Msa0973530	AT4G30250.1	28.571	168	97	5	1	152	8	168	7.17e-13	67.0

Find 28 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAATGAAGAATAGGGATTT+TGG	0.146272	6_4:+5995330	Msa0973530:CDS
ATTTCTCATAGAAGGTTATT+TGG	0.200148	6_4:-5994969	Msa0973520:intron
TGCTTAGAACAAGTGGATTT+TGG	0.205867	6_4:-5995101	Msa0973520:intron
CTCTTACATCCAACATGTTT+TGG	0.206662	6_4:+5995290	Msa0973530:CDS
ATTGAAAATGATTGTGTTCT+TGG	0.272321	6_4:-5995195	Msa0973520:intron
ATCTCCTGATTTCTCATAGA+AGG	0.330595	6_4:-5994977	Msa0973520:intron
AGGCAATAACAATGAAGAAT+AGG	0.370355	6_4:+5995322	Msa0973530:CDS
TAGAAGGTTATTTGGATGTA+AGG	0.371337	6_4:-5994961	Msa0973520:intron
TTGAAAATGATTGTGTTCTT+GGG	0.389511	6_4:-5995194	Msa0973520:intron
GGCAATAACAATGAAGAATA+GGG	0.397758	6_4:+5995323	Msa0973530:CDS
TGAACTCATCTGTGATCTCT+TGG	0.398036	6_4:-5995134	Msa0973520:intron
AGTTGGCACTAAGATATGTT+TGG	0.403404	6_4:-5995032	Msa0973520:intron
ATAACCTTCTATGAGAAATC+AGG	0.414579	6_4:+5994973	Msa0973530:CDS
AGTTCAATGGTGTCAAAGTT+TGG	0.425897	6_4:+5995151	Msa0973530:CDS
TTAACAAATCTACGAAGTGC+TGG	0.478784	6_4:-5994904	Msa0973520:intron
TCATCCATGCTTAGAACAAG+TGG	0.497876	6_4:-5995108	Msa0973520:intron
CTAAAATCTCTCATTGATGA+AGG	0.502280	6_4:-5995375	Msa0973520:intron
CAAAACATGTTGGATGTAAG+AGG	0.509559	6_4:-5995289	Msa0973520:intron
TTCCTTGTTCCAAAACATGT+TGG	0.518629	6_4:-5995299	Msa0973520:intron
AGAAGGTTATTTGGATGTAA+GGG	0.546485	6_4:-5994960	Msa0973520:intron
ACAAATCTACGAAGTGCTGG+TGG	0.569895	6_4:-5994901	Msa0973520:intron
TGGGAAGAATTTCTCATACA+TGG	0.570313	6_4:-5994881	Msa0973520:intron
AAATCCACTTGTTCTAAGCA+TGG	0.575420	6_4:+5995104	Msa0973530:CDS
GAGATCACAGATGAGTTCAA+TGG	0.581658	6_4:+5995138	Msa0973530:CDS
GGCACTAAGATATGTTTGGA+TGG	0.589770	6_4:-5995028	Msa0973520:intron
TGAAAATGATTGTGTTCTTG+GGG	0.597943	6_4:-5995193	Msa0973520:intron
CAAATCTACGAAGTGCTGGT+GGG	0.606728	6_4:-5994900	Msa0973520:intron
TCAATGGTGTCAAAGTTTGG+TGG	0.621281	6_4:+5995154	Msa0973530:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTAATTTAGTGTTGTTGA+TGG	+	chr6_4:5995434-5995453	Msa0973530:CDS	20.0%
!	ATTTCTCATAGAAGGTTATT+TGG	-	chr6_4:5994972-5994991	Msa0973520:intron	25.0%
!	TTGAAAATGATTGTGTTCTT+GGG	-	chr6_4:5995197-5995216	Msa0973520:intron	25.0%
!	ATTGAAAATGATTGTGTTCT+TGG	-	chr6_4:5995198-5995217	Msa0973520:intron	25.0%
!!	TTCATCAATGAGAGATTTTA+GGG	+	chr6_4:5995377-5995396	Msa0973530:CDS	25.0%
	AGAAGGTTATTTGGATGTAA+GGG	-	chr6_4:5994963-5994982	Msa0973520:intron	30.0%
	TAGAAGGTTATTTGGATGTA+AGG	-	chr6_4:5994964-5994983	Msa0973520:intron	30.0%
	ATAACCTTCTATGAGAAATC+AGG	+	chr6_4:5994973-5994992	Msa0973530:CDS	30.0%
	TGAAAATGATTGTGTTCTTG+GGG	-	chr6_4:5995196-5995215	Msa0973520:intron	30.0%
!	ATCTTTTCTCATCTGACTTA+GGG	-	chr6_4:5995224-5995243	Msa0973520:intron	30.0%
!	AATCTTTTCTCATCTGACTT+AGG	-	chr6_4:5995225-5995244	Msa0973520:intron	30.0%
!	ATGTCTTTTATGGAAGGTTA+GGG	-	chr6_4:5995253-5995272	Msa0973520:intron	30.0%
	AGGCAATAACAATGAAGAAT+AGG	+	chr6_4:5995322-5995341	Msa0973530:CDS	30.0%
	GGCAATAACAATGAAGAATA+GGG	+	chr6_4:5995323-5995342	Msa0973530:CDS	30.0%
!	ACAATGAAGAATAGGGATTT+TGG	+	chr6_4:5995330-5995349	Msa0973530:CDS	30.0%
!	CAATGAAGAATAGGGATTTT+GGG	+	chr6_4:5995331-5995350	Msa0973530:CDS	30.0%
!	AATGAAGAATAGGGATTTTG+GGG	+	chr6_4:5995332-5995351	Msa0973530:CDS	30.0%
	CTAAAATCTCTCATTGATGA+AGG	-	chr6_4:5995378-5995397	Msa0973520:intron	30.0%
!	CTTCATCAATGAGAGATTTT+AGG	+	chr6_4:5995376-5995395	Msa0973530:CDS	30.0%
!!	ATGAGAGATTTTAGGGATTT+TGG	+	chr6_4:5995384-5995403	Msa0973530:CDS	30.0%
	TGGGAAGAATTTCTCATACA+TGG	-	chr6_4:5994884-5994903	Msa0973520:intron	35.0%
	TTAACAAATCTACGAAGTGC+TGG	-	chr6_4:5994907-5994926	Msa0973520:intron	35.0%
	ATCTCCTGATTTCTCATAGA+AGG	-	chr6_4:5994980-5994999	Msa0973520:intron	35.0%
	AGTTGGCACTAAGATATGTT+TGG	-	chr6_4:5995035-5995054	Msa0973520:intron	35.0%
!!!	TCTAGCTCTTTTTGAAGAGT+TGG	-	chr6_4:5995052-5995071	Msa0973520:intron	35.0%
!!	TGCTTAGAACAAGTGGATTT+TGG	-	chr6_4:5995104-5995123	Msa0973520:intron	35.0%
!	AAATCCACTTGTTCTAAGCA+TGG	+	chr6_4:5995104-5995123	Msa0973530:CDS	35.0%
!	AGTTCAATGGTGTCAAAGTT+TGG	+	chr6_4:5995151-5995170	Msa0973530:CDS	35.0%
!	GATGTCTTTTATGGAAGGTT+AGG	-	chr6_4:5995254-5995273	Msa0973520:intron	35.0%
!	ATCACGATGTCTTTTATGGA+AGG	-	chr6_4:5995259-5995278	Msa0973520:intron	35.0%
!	TGAGATCACGATGTCTTTTA+TGG	-	chr6_4:5995263-5995282	Msa0973520:intron	35.0%
	CAAAACATGTTGGATGTAAG+AGG	-	chr6_4:5995292-5995311	Msa0973520:intron	35.0%
!	CTCTTACATCCAACATGTTT+TGG	+	chr6_4:5995290-5995309	Msa0973530:CDS	35.0%
!!!	ATCCAACATGTTTTGGAACA+AGG	+	chr6_4:5995297-5995316	Msa0973530:CDS	35.0%
	TTCCTTGTTCCAAAACATGT+TGG	-	chr6_4:5995302-5995321	Msa0973520:intron	35.0%
!!!	ACATGTTTTGGAACAAGGAA+AGG	+	chr6_4:5995302-5995321	Msa0973530:CDS	35.0%
!!	ATAGGGATTTTGGGGATTTT+AGG	+	chr6_4:5995340-5995359	Msa0973530:CDS	35.0%
!!	TAGGGATTTTGGGGATTTTA+GGG	+	chr6_4:5995341-5995360	Msa0973530:CDS	35.0%
!!	TTTGGGGATTTTAGGGATTT+TGG	+	chr6_4:5995348-5995367	Msa0973530:CDS	35.0%
	GGCACTAAGATATGTTTGGA+TGG	-	chr6_4:5995031-5995050	Msa0973520:intron	40.0%
	TCATCCATGCTTAGAACAAG+TGG	-	chr6_4:5995111-5995130	Msa0973520:intron	40.0%
	TGAACTCATCTGTGATCTCT+TGG	-	chr6_4:5995137-5995156	Msa0973520:intron	40.0%
	GAGATCACAGATGAGTTCAA+TGG	+	chr6_4:5995138-5995157	Msa0973530:CDS	40.0%
!	TCAATGGTGTCAAAGTTTGG+TGG	+	chr6_4:5995154-5995173	Msa0973530:CDS	40.0%
!!	CAAATCTACGAAGTGCTGGT+GGG	-	chr6_4:5994903-5994922	Msa0973520:intron	45.0%
!!	ACAAATCTACGAAGTGCTGG+TGG	-	chr6_4:5994904-5994923	Msa0973520:intron	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr6_4	gene	5994856	5995479	5994856	ID=Msa0973530;Name=Msa0973530
chr6_4	mRNA	5994856	5995479	5994856	ID=Msa0973530-mRNA-1;Parent=Msa0973530;Name=Msa0973530-mRNA-1;_AED=0.53;_eAED=0.53;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|207
chr6_4	exon	5994856	5995479	5994856	ID=Msa0973530-mRNA-1:exon:2528;Parent=Msa0973530-mRNA-1
chr6_4	CDS	5994856	5995479	5994856	ID=Msa0973530-mRNA-1:cds;Parent=Msa0973530-mRNA-1

Gene Sequence

>Msa0973530

ATGGCTAGCATAATGTTTGTATATGCCATGTATGAGAAATTCTTCCCACCAGCACTTCGTAGATTTGTTAAAAAATACATTCACAAATTCACAAACTTCATGTACCCTTACATCCAAATAACCTTCTATGAGAAATCAGGAGATCATCTCAAACACAGCAAAACTTACACAACCATCCAAACATATCTTAGTGCCAACTCTTCAAAAAGAGCTAGAAGATTTAAAGCTGAAGTTATCAAAGATAGCCAAAATCCACTTGTTCTAAGCATGGATGACAACCAAGAGATCACAGATGAGTTCAATGGTGTCAAAGTTTGGTGGTCAGCAAATCATATCACCCCAAGAACACAATCATTTTCAATCTACCCTAAGTCAGATGAGAAAAGATTCTTAACCCTAACCTTCCATAAAAGACATCGTGATCTCATCACTACCTCTTACATCCAACATGTTTTGGAACAAGGAAAGGCAATAACAATGAAGAATAGGGATTTTGGGGATTTTAGGGATTTTGGATCTCCTTCATCAATGAGAGATTTTAGGGATTTTGGATCTGATTGCTTAAGTTTCTTCATTTTTTTTAATTTAGTGTTGTTGATGGTGATAATGATGATGAGATTGTGA

Protein sequence

>Msa0973530

MASIMFVYAMYEKFFPPALRRFVKKYIHKFTNFMYPYIQITFYEKSGDHLKHSKTYTTIQTYLSANSSKRARRFKAEVIKDSQNPLVLSMDDNQEITDEFNGVKVWWSANHITPRTQSFSIYPKSDEKRFLTLTFHKRHRDLITTSYIQHVLEQGKAITMKNRDFGDFRDFGSPSSMRDFRDFGSDCLSFFIFFNLVLLMVIMMMRL*