AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0967510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967510 MtrunA17_Chr5g0425371 71.504 379 105 1 1 379 1 376 0.0 575
Msa0967510 MtrunA17_Chr6g0465981 71.883 377 103 1 1 377 1 374 0.0 570
Msa0967510 MtrunA17_Chr6g0465871 71.883 377 103 1 1 377 1 374 0.0 570
Msa0967510 MtrunA17_Chr6g0466081 71.883 377 103 1 1 377 1 374 0.0 570
Msa0967510 MtrunA17_Chr6g0466051 71.693 378 104 1 1 378 1 375 0.0 569
Msa0967510 MtrunA17_Chr6g0465911 71.429 378 105 1 1 378 1 375 0.0 567
Msa0967510 MtrunA17_Chr7g0236991 71.159 371 104 1 1 371 1 368 0.0 558
Msa0967510 MtrunA17_Chr7g0237001 70.889 371 105 1 1 371 1 368 0.0 556
Msa0967510 MtrunA17_Chr7g0237041 70.889 371 105 1 1 371 1 368 0.0 556
Msa0967510 MtrunA17_Chr7g0237081 70.889 371 105 1 1 371 1 368 0.0 556
Msa0967510 MtrunA17_Chr7g0237051 70.889 371 105 1 1 371 1 368 0.0 556
Msa0967510 MtrunA17_Chr6g0465441 69.048 378 114 1 1 378 1 375 0.0 553
Msa0967510 MtrunA17_Chr6g0451711 68.158 380 120 1 1 379 1 380 0.0 542
Msa0967510 MtrunA17_Chr3g0105071 67.546 379 122 1 1 378 1 379 0.0 537
Msa0967510 MtrunA17_Chr3g0082691 68.852 366 113 1 12 376 8 373 0.0 527
Msa0967510 MtrunA17_Chr6g0466111 73.451 339 90 0 39 377 1 339 0.0 523
Msa0967510 MtrunA17_Chr6g0465851 72.941 340 92 0 39 378 1 340 0.0 520
Msa0967510 MtrunA17_Chr6g0465931 73.156 339 91 0 39 377 1 339 0.0 519
Msa0967510 MtrunA17_Chr5g0431571 70.137 365 108 1 12 375 4 368 0.0 518
Msa0967510 MtrunA17_Chr4g0024691 65.591 372 128 0 7 378 12 383 0.0 516
Msa0967510 MtrunA17_Chr5g0431651 66.304 368 122 2 13 378 7 374 2.78e-177 504
Msa0967510 MtrunA17_Chr7g0236951 71.787 319 90 0 59 377 1 319 8.15e-174 486
Msa0967510 MtrunA17_Chr7g0237031 71.787 319 90 0 59 377 1 319 2.87e-173 485
Msa0967510 MtrunA17_Chr7g0236961 72.524 313 86 0 59 371 1 313 8.57e-171 486
Msa0967510 MtrunA17_Chr7g0237011 72.524 313 86 0 59 371 1 313 2.78e-170 484
Msa0967510 MtrunA17_Chr4g0042111 59.569 371 147 2 2 370 7 376 5.08e-160 461
Msa0967510 MtrunA17_Chr5g0445511 69.869 229 69 0 151 379 1 229 2.03e-119 345
Msa0967510 MtrunA17_Chr2g0319041 47.740 354 182 3 15 365 12 365 6.83e-119 355
Msa0967510 MtrunA17_Chr8g0336181 48.997 349 175 2 13 358 9 357 6.23e-116 348
Msa0967510 MtrunA17_Chr4g0008621 45.479 365 196 3 15 376 19 383 1.78e-113 338
Msa0967510 MtrunA17_Chr7g0236971 70.833 216 63 0 156 371 1 216 1.05e-111 332
Msa0967510 MtrunA17_Chr2g0319051 46.526 331 175 2 48 376 4 334 1.30e-107 325
Msa0967510 MtrunA17_Chr6g0466021 71.078 204 56 1 1 204 1 201 1.03e-103 304
Msa0967510 MtrunA17_Chr7g0237071 70.297 202 60 0 59 260 1 202 5.16e-99 293
Msa0967510 MtrunA17_Chr4g0023911 40.108 369 209 5 13 377 41 401 1.08e-87 276
Msa0967510 MtrunA17_Chr2g0310331 42.424 363 199 6 17 377 53 407 4.25e-86 272
Msa0967510 MtrunA17_Chr8g0357411 37.669 369 223 3 10 377 48 410 1.12e-78 253
Msa0967510 MtrunA17_Chr2g0323131 40.439 319 176 3 49 364 67 374 1.74e-77 249
Msa0967510 MtrunA17_Chr2g0323151 41.414 297 161 2 71 364 4 290 4.36e-73 235
Msa0967510 MtrunA17_Chr2g0319071 34.862 327 167 6 53 377 28 310 8.66e-61 203
Msa0967510 MtrunA17_Chr7g0236981 67.407 135 41 1 1 135 1 132 1.71e-60 192
Msa0967510 MtrunA17_Chr4g0048911 35.948 306 190 4 55 357 61 363 1.37e-58 199
Msa0967510 MtrunA17_Chr4g0048921 36.420 324 197 5 44 365 64 380 3.37e-58 198
Msa0967510 MtrunA17_Chr7g0233861 64.444 135 39 1 196 330 33 158 4.38e-55 179
Msa0967510 MtrunA17_Chr2g0280581 33.333 330 201 5 47 365 9 330 5.21e-53 183
Msa0967510 MtrunA17_Chr2g0310401 38.400 250 151 2 130 377 5 253 1.52e-49 172
Msa0967510 MtrunA17_Chr3g0109001 32.177 317 208 6 54 365 13 327 9.19e-45 161
Msa0967510 MtrunA17_Chr3g0105691 32.781 302 200 3 54 353 46 346 1.95e-44 161
Msa0967510 MtrunA17_Chr3g0109021 32.278 316 208 6 54 365 13 326 2.32e-44 160
Msa0967510 MtrunA17_Chr3g0108941 31.861 317 209 6 54 365 13 327 3.30e-44 160
Msa0967510 MtrunA17_Chr3g0109031 31.429 315 212 4 54 365 13 326 3.95e-44 159
Msa0967510 MtrunA17_Chr3g0108981 31.650 297 199 3 73 365 6 302 6.77e-43 155
Msa0967510 MtrunA17_Chr3g0108961 31.650 297 199 3 73 365 6 302 6.77e-43 155
Msa0967510 MtrunA17_Chr6g0477111 31.313 297 199 5 73 365 6 301 6.25e-42 153
Msa0967510 MtrunA17_Chr8g0357221 35.743 249 153 3 10 257 48 290 6.72e-42 148
Msa0967510 MtrunA17_Chr5g0445521 66.327 98 33 0 1 98 1 98 6.91e-42 142
Msa0967510 MtrunA17_Chr3g0108991 31.013 316 212 6 54 365 13 326 8.92e-41 150
Msa0967510 MtrunA17_Chr3g0108951 31.013 316 212 6 54 365 13 326 8.92e-41 150
Msa0967510 MtrunA17_Chr3g0099031 31.329 316 211 6 54 365 13 326 2.21e-40 149
Msa0967510 MtrunA17_Chr3g0108971 30.976 297 200 5 73 365 6 301 4.83e-40 148
Msa0967510 MtrunA17_Chr3g0109011 30.405 296 203 3 73 365 6 301 1.16e-39 147
Msa0967510 MtrunA17_Chr5g0445401 69.149 94 27 1 166 257 1 94 3.85e-36 127
Msa0967510 MtrunA17_Chr2g0319081 40.690 145 79 3 233 376 120 258 3.76e-32 125
Msa0967510 MtrunA17_Chr2g0319081 40.984 122 71 1 15 135 19 140 5.15e-24 102
Msa0967510 MtrunA17_Chr6g0477121 30.932 236 159 4 133 365 10 244 2.02e-31 123
Msa0967510 MtrunA17_Chr7g0237061 73.333 75 20 0 297 371 1 75 2.55e-31 119
Msa0967510 MtrunA17_Chr2g0310121 28.615 325 199 9 69 366 274 592 3.70e-30 122
Msa0967510 MtrunA17_Chr3g0101681 28.354 328 202 9 69 369 275 596 2.75e-29 119
Msa0967510 MtrunA17_Chr6g0466031 64.634 82 29 0 297 378 1 82 9.72e-27 107
Msa0967510 MtrunA17_Chr7g0259881 27.675 271 162 7 73 313 232 498 5.24e-25 107
Msa0967510 MtrunA17_Chr7g0218001 30.000 270 155 7 73 310 349 616 2.40e-24 105
Msa0967510 MtrunA17_Chr7g0217361 40.909 110 65 0 255 364 8 117 1.11e-21 94.4
Msa0967510 MtrunA17_Chr7g0217351 30.594 219 139 6 46 258 62 273 1.61e-21 93.6
Msa0967510 MtrunA17_Chr8g0357231 41.250 80 47 0 298 377 1 80 1.06e-15 76.3
Msa0967510 MtrunA17_Chr7g0218041 30.455 220 123 7 120 311 3 220 1.11e-14 75.1
Msa0967510 MtrunA17_Chr2g0310411 32.787 122 74 3 10 131 51 164 4.77e-13 66.6
Msa0967510 MtrunA17_Chr2g0281651 30.597 134 88 3 233 365 43 172 1.02e-12 68.9
Msa0967510 MtrunA17_Chr5g0411111 36.220 127 66 5 120 235 3 125 1.20e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967510 AT1G70080.1 38.684 380 222 7 8 379 57 433 3.44e-88 278
Msa0967510 AT5G23960.2 37.079 356 220 3 13 366 3 356 1.22e-87 275
Msa0967510 AT5G23960.1 37.047 359 218 5 13 366 3 358 2.44e-86 272
Msa0967510 AT3G14490.1 38.315 368 217 5 17 378 60 423 1.13e-84 269
Msa0967510 AT1G31950.2 37.568 370 209 5 22 379 70 429 8.94e-84 265
Msa0967510 AT1G31950.3 37.568 370 209 5 22 379 70 429 4.27e-83 265
Msa0967510 AT1G31950.1 38.017 363 216 4 22 379 70 428 2.89e-82 263
Msa0967510 AT3G14540.2 38.398 362 216 3 22 379 65 423 3.47e-82 260
Msa0967510 AT3G14540.1 38.398 362 216 3 22 379 65 423 3.63e-81 260
Msa0967510 AT3G14520.1 38.122 362 215 5 22 378 68 425 2.80e-80 258
Msa0967510 AT4G15870.1 37.500 360 213 7 25 376 75 430 1.09e-76 248
Msa0967510 AT1G33750.1 36.464 362 216 6 24 379 73 426 8.51e-76 246
Msa0967510 AT3G29410.1 36.986 365 224 5 17 378 64 425 1.27e-75 245
Msa0967510 AT5G44630.1 35.657 373 223 6 18 379 14 380 2.64e-75 243
Msa0967510 AT4G20210.1 35.580 371 226 7 17 379 58 423 5.89e-72 236
Msa0967510 AT3G32030.1 34.903 361 228 5 22 378 68 425 5.00e-71 233
Msa0967510 AT3G29410.2 38.585 311 187 3 70 378 12 320 9.69e-71 230
Msa0967510 AT4G16740.2 34.828 379 227 8 12 379 21 390 1.83e-70 226
Msa0967510 AT3G29110.1 36.364 363 218 7 17 371 55 412 3.35e-69 228
Msa0967510 AT4G16730.1 37.092 337 194 6 52 377 27 356 7.62e-69 226
Msa0967510 AT4G13300.1 36.011 361 221 6 25 379 20 376 9.94e-69 226
Msa0967510 AT4G20200.1 36.441 354 194 8 47 379 84 427 2.01e-68 226
Msa0967510 AT4G16740.1 34.667 375 226 7 12 376 21 386 4.66e-68 224
Msa0967510 AT2G23230.2 35.657 373 226 8 16 379 55 422 6.80e-68 225
Msa0967510 AT2G23230.1 35.946 370 223 8 16 376 55 419 7.69e-68 225
Msa0967510 AT3G29190.1 35.588 340 211 5 45 378 86 423 1.77e-67 224
Msa0967510 AT1G66020.2 34.332 367 232 6 17 377 56 419 2.62e-67 219
Msa0967510 AT4G16730.2 37.658 316 180 5 73 377 1 310 4.40e-67 220
Msa0967510 AT4G20230.1 35.775 355 213 8 25 370 70 418 6.00e-67 223
Msa0967510 AT4G20230.2 35.775 355 213 8 25 370 82 430 7.33e-67 223
Msa0967510 AT2G24210.1 34.933 375 228 7 10 376 39 405 3.58e-66 220
Msa0967510 AT1G66020.1 33.875 369 235 6 17 379 56 421 3.83e-65 218
Msa0967510 AT4G13280.1 36.592 358 215 7 27 377 22 374 1.16e-64 214
Msa0967510 AT4G13280.2 36.592 358 215 7 27 377 22 374 1.54e-64 215
Msa0967510 AT3G25820.2 33.333 354 214 8 15 357 45 387 6.94e-61 202
Msa0967510 AT4G16740.4 34.650 329 197 6 12 330 21 341 1.31e-59 197
Msa0967510 AT4G16740.3 34.650 329 197 6 12 330 21 341 1.31e-59 197
Msa0967510 AT3G25820.1 33.333 354 214 8 15 357 45 387 2.41e-59 202
Msa0967510 AT3G25830.1 33.333 354 214 8 15 357 45 387 2.41e-59 202
Msa0967510 AT1G48800.1 33.780 373 231 9 16 378 62 428 1.06e-58 201
Msa0967510 AT1G48820.1 34.648 355 199 9 17 366 63 389 2.68e-56 193
Msa0967510 AT3G25810.1 32.759 348 219 6 15 352 45 387 7.56e-56 193
Msa0967510 AT3G25820.4 32.416 327 202 7 15 330 45 363 1.07e-53 182
Msa0967510 AT3G25820.3 32.416 327 202 7 15 330 45 363 1.07e-53 182
Msa0967510 AT1G61680.1 33.016 315 200 3 54 363 77 385 3.79e-51 180
Msa0967510 AT1G61680.2 32.381 315 195 4 54 363 77 378 8.49e-48 171
Msa0967510 AT5G48110.1 44.785 163 88 2 215 376 1 162 1.53e-42 152
Msa0967510 AT4G02780.1 29.688 256 150 6 73 300 334 587 1.35e-23 103
Msa0967510 AT1G79460.1 27.463 335 195 13 69 369 273 593 4.52e-23 101
Msa0967510 AT1G61120.1 26.837 313 191 9 68 356 286 584 2.51e-20 93.6

Find 55 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTCTTGCTGTGCTATTT+AGG 0.103456 6_3:+97259381 Msa0967510:CDS
TCTACTTGCATTGGCAAAAT+TGG 0.119753 6_3:+97260527 Msa0967510:CDS
TTCTCTTGCTGTGCTATTTA+GGG 0.189824 6_3:+97259382 Msa0967510:CDS
GGTTCATGGTGAAAATATTT+TGG 0.219650 6_3:+97260329 Msa0967510:CDS
TTTCCTTGCTTGAGAATATT+GGG 0.241792 6_3:-97260812 None:intergenic
ATGCAAGTAGAATTTCATTA+TGG 0.248221 6_3:-97260515 None:intergenic
AGAAGAAATTAGTCAAGTTT+TGG 0.262222 6_3:+97259301 Msa0967510:CDS
TACAAGAGTTATGTTCAAAA+TGG 0.269625 6_3:+97259332 Msa0967510:CDS
GTCATCAATGTATGATAAAA+TGG 0.310334 6_3:-97260854 None:intergenic
CAGATTTCCACCCTAGCGTT+TGG 0.311610 6_3:+97258925 Msa0967510:CDS
AGATTTCCACCCTAGCGTTT+GGG 0.325288 6_3:+97258926 Msa0967510:CDS
TTCCTTGCTTGTCCTTGAAT+TGG 0.334271 6_3:-97260239 None:intergenic
TGCAAAACCGATTTCTCATT+AGG 0.335406 6_3:-97258891 None:intergenic
TAATTGCTTGGAGGATCTTC+CGG 0.335446 6_3:+97261210 Msa0967510:CDS
TGTGCCGCAATATTCATATC+AGG 0.348479 6_3:-97259161 None:intergenic
CTTCAATCCTAATGAGAAAT+CGG 0.354762 6_3:+97258884 Msa0967510:CDS
TCAGTATGCTTCAGAATCTA+TGG 0.376448 6_3:+97258962 Msa0967510:CDS
TGCAAGTAGAATTTCATTAT+GGG 0.387262 6_3:-97260514 None:intergenic
GATTGACTCGATATGTCGTT+TGG 0.393872 6_3:+97259259 Msa0967510:CDS
TAAAGCAACTTCGTGTAATC+CGG 0.404734 6_3:-97261229 None:intergenic
GTTGCTTTATAAATCACTCT+TGG 0.404999 6_3:+97261243 Msa0967510:CDS
TAGGTGGGATATTAATTGCT+TGG 0.452782 6_3:+97261198 Msa0967510:intron
TAATGAAATTCTACTTGCAT+TGG 0.460272 6_3:+97260518 Msa0967510:CDS
TTCTGGTCACCTTCAGTCCA+TGG 0.466911 6_3:+97260371 Msa0967510:CDS
GATGACACATATGATGCATA+TGG 0.467097 6_3:+97260871 Msa0967510:CDS
GAGCAAGAAATGAAGAAAGA+AGG 0.470516 6_3:+97261283 Msa0967510:CDS
GGATGAATCTTGCTCATACA+TGG 0.486888 6_3:-97260493 None:intergenic
ATTGACTCGATATGTCGTTT+GGG 0.487347 6_3:+97259260 Msa0967510:CDS
ATATTGGGGCTCAAAATATA+CGG 0.495144 6_3:-97260797 None:intergenic
CATGGACTTTATGTTTCACC+TGG 0.496318 6_3:+97259416 Msa0967510:CDS
ATATAGGTGGTGGAAGGAGT+TGG 0.505587 6_3:+97260711 Msa0967510:intron
ATGACTCAATCGGGTGGCCA+TGG 0.509233 6_3:-97260388 None:intergenic
AGACGTGTTCAACCAATTCA+AGG 0.513117 6_3:+97260227 Msa0967510:intron
TGCAAGAGAATGACTCAATC+GGG 0.519865 6_3:-97260397 None:intergenic
TAACTTCTTGCCACTAGCCT+AGG 0.521919 6_3:-97260465 None:intergenic
AGGAAATTTCAGTGAAACAT+TGG 0.522145 6_3:+97260257 Msa0967510:CDS
GCCTCACATATGATGGTTCA+TGG 0.522278 6_3:+97260315 Msa0967510:CDS
ATGATGTGCTAACCTTTCAA+TGG 0.523871 6_3:-97260926 None:intergenic
TTGCAAGAGAATGACTCAAT+CGG 0.526770 6_3:-97260398 None:intergenic
GTGGGATATTAATTGCTTGG+AGG 0.529634 6_3:+97261201 Msa0967510:CDS
TTCCTTGCTTGAGAATATTG+GGG 0.534381 6_3:-97260811 None:intergenic
AACATTGGTCGTAGATGTTG+AGG 0.541350 6_3:+97260272 Msa0967510:CDS
AGCCCCAATATTCTCAAGCA+AGG 0.547455 6_3:+97260809 Msa0967510:CDS
ATCGGGTGGCCATGGACTGA+AGG 0.566605 6_3:-97260380 None:intergenic
CTTTGTATATAGGTGGTGGA+AGG 0.567617 6_3:+97260705 Msa0967510:intron
GTATGAAGCCTCACATATGA+TGG 0.567934 6_3:+97260308 Msa0967510:CDS
ACTACTCTATCTCGTGCATA+CGG 0.590301 6_3:-97260751 None:intergenic
GATTTCCACCCTAGCGTTTG+GGG 0.596026 6_3:+97258927 Msa0967510:CDS
TTTAGGGTGTTGAGACAACA+TGG 0.599316 6_3:+97259398 Msa0967510:CDS
AATCTCCCCAAACGCTAGGG+TGG 0.601357 6_3:-97258932 None:intergenic
AACCAATTCAAGGACAAGCA+AGG 0.613328 6_3:+97260237 Msa0967510:CDS
GGAACCTGATATGAATATTG+CGG 0.628405 6_3:+97259157 Msa0967510:intron
ACATTGGTCGTAGATGTTGA+GGG 0.633793 6_3:+97260273 Msa0967510:CDS
ACCATGAACCATCATATGTG+AGG 0.634158 6_3:-97260316 None:intergenic
AAGAGAATGACTCAATCGGG+TGG 0.693305 6_3:-97260394 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGTAAATAAATAGTATA+AGG - chr6_3:97259115-97259134 None:intergenic 15.0%
!! ACAAATTGATAAACTAAAAA+AGG + chr6_3:97259181-97259200 Msa0967510:CDS 15.0%
!!! ACTTATTCTCATTTTTTTTA+AGG + chr6_3:97259452-97259471 Msa0967510:intron 15.0%
!!! CAGTTTTTATGTTTTTAATT+TGG + chr6_3:97259532-97259551 Msa0967510:intron 15.0%
!! TAATATTTGACTATATTCAT+GGG + chr6_3:97259644-97259663 Msa0967510:intron 15.0%
!! TAATATGAATGTCTAAAAAA+TGG + chr6_3:97259673-97259692 Msa0967510:intron 15.0%
!!! AATTCTTTTTTTACTAAAAG+TGG - chr6_3:97259703-97259722 None:intergenic 15.0%
!! GATAATAATTAAAAGAAATG+AGG - chr6_3:97259831-97259850 None:intergenic 15.0%
!! AATATTAATGTTATACATGT+AGG + chr6_3:97259998-97260017 Msa0967510:intron 15.0%
!! ATAAATTAATATGTCTCTAA+TGG + chr6_3:97260167-97260186 Msa0967510:intron 15.0%
!!! TATTTTTTGTCTTTGTATAT+AGG + chr6_3:97260695-97260714 Msa0967510:intron 15.0%
!! GATTAAAGATAAATTTCATA+TGG - chr6_3:97261005-97261024 None:intergenic 15.0%
!! AATTCTTTAAATCAACAACA+AGG + chr6_3:97258990-97259009 Msa0967510:intron 20.0%
!! AATTGATAAACTAAAAAAGG+AGG + chr6_3:97259184-97259203 Msa0967510:CDS 20.0%
!! GTAATATTTGACTATATTCA+TGG + chr6_3:97259643-97259662 Msa0967510:intron 20.0%
!! ATTGGATACATATTAGATAT+TGG + chr6_3:97259791-97259810 Msa0967510:intron 20.0%
!!! AAGATAATAATTTTTTGCCA+GGG - chr6_3:97259927-97259946 None:intergenic 20.0%
!!! AAAGATAATAATTTTTTGCC+AGG - chr6_3:97259928-97259947 None:intergenic 20.0%
!! AAGTTTCTTACTTAATAAGA+TGG - chr6_3:97260066-97260085 None:intergenic 20.0%
!! ATGGTTGTTTAATATTATTG+TGG + chr6_3:97260186-97260205 Msa0967510:intron 20.0%
!!! ATTTTGTTTTAAATTAGGCA+GGG - chr6_3:97260623-97260642 None:intergenic 20.0%
!!! TATTTTGTTTTAAATTAGGC+AGG - chr6_3:97260624-97260643 None:intergenic 20.0%
!! ACAAAGACAAAAAATAGTAT+AGG - chr6_3:97260691-97260710 None:intergenic 20.0%
!!! TGAATTAGAACTTTTTACAA+AGG + chr6_3:97260903-97260922 Msa0967510:CDS 20.0%
!!! TTGCAATCAAAATGATTTTT+AGG + chr6_3:97261179-97261198 Msa0967510:intron 20.0%
!!! AATCAAAATGATTTTTAGGT+GGG + chr6_3:97261183-97261202 Msa0967510:intron 20.0%
!! AGAAGAAATTAGTCAAGTTT+TGG + chr6_3:97259301-97259320 Msa0967510:CDS 25.0%
! TACAAGAGTTATGTTCAAAA+TGG + chr6_3:97259332-97259351 Msa0967510:CDS 25.0%
!! TAAGTTGAGATATATATACC+AGG - chr6_3:97259437-97259456 None:intergenic 25.0%
!!! TGAGTGTCTTTTTTTTTGAT+TGG + chr6_3:97259773-97259792 Msa0967510:intron 25.0%
! GACAATTCATAAAATATGCA+CGG + chr6_3:97259888-97259907 Msa0967510:intron 25.0%
! TGCAAGTAGAATTTCATTAT+GGG - chr6_3:97260517-97260536 None:intergenic 25.0%
! ATGCAAGTAGAATTTCATTA+TGG - chr6_3:97260518-97260537 None:intergenic 25.0%
! TAATGAAATTCTACTTGCAT+TGG + chr6_3:97260518-97260537 Msa0967510:CDS 25.0%
!! TTCCAAATTCTTTTTGATGT+AGG - chr6_3:97260572-97260591 None:intergenic 25.0%
!! TTTTTGTCTTTGTATATAGG+TGG + chr6_3:97260698-97260717 Msa0967510:intron 25.0%
!!! AGTAGTAGAATGTTGTTTTT+GGG + chr6_3:97260768-97260787 Msa0967510:CDS 25.0%
! GTCATCAATGTATGATAAAA+TGG - chr6_3:97260857-97260876 None:intergenic 25.0%
!!! ATGGTTTTAACTCTAAAGTT+TGG - chr6_3:97260986-97261005 None:intergenic 25.0%
!!! CAATCAAAATGATTTTTAGG+TGG + chr6_3:97261182-97261201 Msa0967510:intron 25.0%
!!! ACTCTTACTTCTTTTAAGAA+GGG - chr6_3:97261328-97261347 None:intergenic 25.0%
!!! AACTCTTACTTCTTTTAAGA+AGG - chr6_3:97261329-97261348 None:intergenic 25.0%
CTTCAATCCTAATGAGAAAT+CGG + chr6_3:97258884-97258903 Msa0967510:CDS 30.0%
! GAATCTGGGATAATATTAAG+TGG - chr6_3:97259076-97259095 None:intergenic 30.0%
AATGTGAGTAACTTGAATCT+GGG - chr6_3:97259090-97259109 None:intergenic 30.0%
AAATGTGAGTAACTTGAATC+TGG - chr6_3:97259091-97259110 None:intergenic 30.0%
!! TGCATGTTTTGAATACATAC+AGG + chr6_3:97259136-97259155 Msa0967510:intron 30.0%
TTAAAAACAGAGAAACCCTT+GGG + chr6_3:97259224-97259243 Msa0967510:CDS 30.0%
TAATAAGATGGCTACCATAT+GGG - chr6_3:97260054-97260073 None:intergenic 30.0%
TTAATAAGATGGCTACCATA+TGG - chr6_3:97260055-97260074 None:intergenic 30.0%
AGGAAATTTCAGTGAAACAT+TGG + chr6_3:97260257-97260276 Msa0967510:CDS 30.0%
! GGTTCATGGTGAAAATATTT+TGG + chr6_3:97260329-97260348 Msa0967510:CDS 30.0%
!! TGGTGAAAATATTTTGGAAG+AGG + chr6_3:97260335-97260354 Msa0967510:CDS 30.0%
!! AAATATTTTGGAAGAGGCTT+TGG + chr6_3:97260341-97260360 Msa0967510:CDS 30.0%
! ATTCTTTTTGATGTAGGCTT+TGG - chr6_3:97260566-97260585 None:intergenic 30.0%
AGCCTACATCAAAAAGAATT+TGG + chr6_3:97260567-97260586 Msa0967510:CDS 30.0%
!!! GAGTAGTAGAATGTTGTTTT+TGG + chr6_3:97260767-97260786 Msa0967510:CDS 30.0%
ATATTGGGGCTCAAAATATA+CGG - chr6_3:97260800-97260819 None:intergenic 30.0%
TTTCCTTGCTTGAGAATATT+GGG - chr6_3:97260815-97260834 None:intergenic 30.0%
! TTTTCCTTGCTTGAGAATAT+TGG - chr6_3:97260816-97260835 None:intergenic 30.0%
! TTTTTACAAAGGCCATTGAA+AGG + chr6_3:97260914-97260933 Msa0967510:CDS 30.0%
CTAGTCATCAAGTTTAGATT+TGG + chr6_3:97261024-97261043 Msa0967510:intron 30.0%
AATTTAACCATCTCCAATCT+TGG + chr6_3:97261060-97261079 Msa0967510:intron 30.0%
ATTGAATTAGCAACCAAGAT+TGG - chr6_3:97261076-97261095 None:intergenic 30.0%
! GTTGCTTTATAAATCACTCT+TGG + chr6_3:97261243-97261262 Msa0967510:CDS 30.0%
!! CTCTTACTTCTTTTAAGAAG+GGG - chr6_3:97261327-97261346 None:intergenic 30.0%
! TGCAAAACCGATTTCTCATT+AGG - chr6_3:97258894-97258913 None:intergenic 35.0%
TCAGTATGCTTCAGAATCTA+TGG + chr6_3:97258962-97258981 Msa0967510:CDS 35.0%
GGAACCTGATATGAATATTG+CGG + chr6_3:97259157-97259176 Msa0967510:intron 35.0%
CTTAAAAACAGAGAAACCCT+TGG + chr6_3:97259223-97259242 Msa0967510:CDS 35.0%
!!! ATCAAGTTGACTTTTCCCAA+GGG - chr6_3:97259242-97259261 None:intergenic 35.0%
!!! AATCAAGTTGACTTTTCCCA+AGG - chr6_3:97259243-97259262 None:intergenic 35.0%
!! ATTGACTCGATATGTCGTTT+GGG + chr6_3:97259260-97259279 Msa0967510:CDS 35.0%
!! ATTCTCTTGCTGTGCTATTT+AGG + chr6_3:97259381-97259400 Msa0967510:CDS 35.0%
!! TTCTCTTGCTGTGCTATTTA+GGG + chr6_3:97259382-97259401 Msa0967510:CDS 35.0%
TTTCGTGAGCTTAATTCAGT+TGG + chr6_3:97259861-97259880 Msa0967510:intron 35.0%
TGAGCTTAATTCAGTTGGTT+TGG + chr6_3:97259866-97259885 Msa0967510:intron 35.0%
TTGCAAGAGAATGACTCAAT+CGG - chr6_3:97260401-97260420 None:intergenic 35.0%
! TCTACTTGCATTGGCAAAAT+TGG + chr6_3:97260527-97260546 Msa0967510:CDS 35.0%
TTGTCTTTGTATATAGGTGG+TGG + chr6_3:97260701-97260720 Msa0967510:intron 35.0%
TTCCTTGCTTGAGAATATTG+GGG - chr6_3:97260814-97260833 None:intergenic 35.0%
GATGACACATATGATGCATA+TGG + chr6_3:97260871-97260890 Msa0967510:CDS 35.0%
ATGATGTGCTAACCTTTCAA+TGG - chr6_3:97260929-97260948 None:intergenic 35.0%
TAGGTGGGATATTAATTGCT+TGG + chr6_3:97261198-97261217 Msa0967510:intron 35.0%
TAAAGCAACTTCGTGTAATC+CGG - chr6_3:97261232-97261251 None:intergenic 35.0%
GAGCAAGAAATGAAGAAAGA+AGG + chr6_3:97261283-97261302 Msa0967510:CDS 35.0%
!! TTCTTTTAAGAAGGGGTCAA+GGG - chr6_3:97261320-97261339 None:intergenic 35.0%
AAAAATCTCCCCAAACGCTA+GGG - chr6_3:97258938-97258957 None:intergenic 40.0%
AAAAAATCTCCCCAAACGCT+AGG - chr6_3:97258939-97258958 None:intergenic 40.0%
TGTGCCGCAATATTCATATC+AGG - chr6_3:97259164-97259183 None:intergenic 40.0%
!! GATTGACTCGATATGTCGTT+TGG + chr6_3:97259259-97259278 Msa0967510:CDS 40.0%
!! TTTAGGGTGTTGAGACAACA+TGG + chr6_3:97259398-97259417 Msa0967510:CDS 40.0%
CATGGACTTTATGTTTCACC+TGG + chr6_3:97259416-97259435 Msa0967510:CDS 40.0%
TCATAAAATATGCACGGTCC+AGG + chr6_3:97259894-97259913 Msa0967510:intron 40.0%
CATAAAATATGCACGGTCCA+GGG + chr6_3:97259895-97259914 Msa0967510:intron 40.0%
CTTAGACCAGTTTACCCATA+TGG + chr6_3:97260037-97260056 Msa0967510:intron 40.0%
AGACGTGTTCAACCAATTCA+AGG + chr6_3:97260227-97260246 Msa0967510:intron 40.0%
AACCAATTCAAGGACAAGCA+AGG + chr6_3:97260237-97260256 Msa0967510:CDS 40.0%
TTCCTTGCTTGTCCTTGAAT+TGG - chr6_3:97260242-97260261 None:intergenic 40.0%
AACATTGGTCGTAGATGTTG+AGG + chr6_3:97260272-97260291 Msa0967510:CDS 40.0%
ACATTGGTCGTAGATGTTGA+GGG + chr6_3:97260273-97260292 Msa0967510:CDS 40.0%
GTATGAAGCCTCACATATGA+TGG + chr6_3:97260308-97260327 Msa0967510:CDS 40.0%
ACCATGAACCATCATATGTG+AGG - chr6_3:97260319-97260338 None:intergenic 40.0%
TGCAAGAGAATGACTCAATC+GGG - chr6_3:97260400-97260419 None:intergenic 40.0%
GGATGAATCTTGCTCATACA+TGG - chr6_3:97260496-97260515 None:intergenic 40.0%
!! CTTTGTATATAGGTGGTGGA+AGG + chr6_3:97260705-97260724 Msa0967510:intron 40.0%
ACTACTCTATCTCGTGCATA+CGG - chr6_3:97260754-97260773 None:intergenic 40.0%
TTAGCAACCAAGATTGGAGA+TGG - chr6_3:97261070-97261089 None:intergenic 40.0%
GTGGGATATTAATTGCTTGG+AGG + chr6_3:97261201-97261220 Msa0967510:CDS 40.0%
TAATTGCTTGGAGGATCTTC+CGG + chr6_3:97261210-97261229 Msa0967510:CDS 40.0%
!! CTTCTTTTAAGAAGGGGTCA+AGG - chr6_3:97261321-97261340 None:intergenic 40.0%
AGATTTCCACCCTAGCGTTT+GGG + chr6_3:97258926-97258945 Msa0967510:CDS 45.0%
TGGCTACCATATGGGTAAAC+TGG - chr6_3:97260046-97260065 None:intergenic 45.0%
GCCTCACATATGATGGTTCA+TGG + chr6_3:97260315-97260334 Msa0967510:CDS 45.0%
!!! GAGGCTTTGGCTTTTACTTC+TGG + chr6_3:97260354-97260373 Msa0967510:CDS 45.0%
AAGAGAATGACTCAATCGGG+TGG - chr6_3:97260397-97260416 None:intergenic 45.0%
CAAAAACTTGCCTAGGCTAG+TGG + chr6_3:97260455-97260474 Msa0967510:CDS 45.0%
! TAACTTCTTGCCACTAGCCT+AGG - chr6_3:97260468-97260487 None:intergenic 45.0%
!! GTTTTAAATTAGGCAGGGCG+TGG - chr6_3:97260618-97260637 None:intergenic 45.0%
!! ATATAGGTGGTGGAAGGAGT+TGG + chr6_3:97260711-97260730 Msa0967510:intron 45.0%
AGCCCCAATATTCTCAAGCA+AGG + chr6_3:97260809-97260828 Msa0967510:CDS 45.0%
!!! TTTATATTTTGTTTTAAATT+AGG - chr6_3:97260628-97260647 None:intergenic 5.0%
CAGATTTCCACCCTAGCGTT+TGG + chr6_3:97258925-97258944 Msa0967510:CDS 50.0%
GATTTCCACCCTAGCGTTTG+GGG + chr6_3:97258927-97258946 Msa0967510:CDS 50.0%
! TTTTGCCAGGGTTTGAACCC+TGG - chr6_3:97259915-97259934 None:intergenic 50.0%
TTCTGGTCACCTTCAGTCCA+TGG + chr6_3:97260371-97260390 Msa0967510:CDS 50.0%
CTCTGCGCAAAAACTTGCCT+AGG + chr6_3:97260448-97260467 Msa0967510:CDS 50.0%
AATCTCCCCAAACGCTAGGG+TGG - chr6_3:97258935-97258954 None:intergenic 55.0%
ATGACTCAATCGGGTGGCCA+TGG - chr6_3:97260391-97260410 None:intergenic 55.0%
ACGGTCCAGGGTTCAAACCC+TGG + chr6_3:97259907-97259926 Msa0967510:intron 60.0%
! ATCGGGTGGCCATGGACTGA+AGG - chr6_3:97260383-97260402 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 97258867 97261351 97258867 ID=Msa0967510;Name=Msa0967510
chr6_3 mRNA 97258867 97261351 97258867 ID=Msa0967510-mRNA-1;Parent=Msa0967510;Name=Msa0967510-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|5|0|379
chr6_3 exon 97258867 97258983 97258867 ID=Msa0967510-mRNA-1:exon:18023;Parent=Msa0967510-mRNA-1
chr6_3 exon 97259158 97259437 97259158 ID=Msa0967510-mRNA-1:exon:18024;Parent=Msa0967510-mRNA-1
chr6_3 exon 97260229 97260601 97260229 ID=Msa0967510-mRNA-1:exon:18025;Parent=Msa0967510-mRNA-1
chr6_3 exon 97260717 97260935 97260717 ID=Msa0967510-mRNA-1:exon:18026;Parent=Msa0967510-mRNA-1
chr6_3 exon 97261201 97261351 97261201 ID=Msa0967510-mRNA-1:exon:18027;Parent=Msa0967510-mRNA-1
chr6_3 CDS 97258867 97258983 97258867 ID=Msa0967510-mRNA-1:cds;Parent=Msa0967510-mRNA-1
chr6_3 CDS 97259158 97259437 97259158 ID=Msa0967510-mRNA-1:cds;Parent=Msa0967510-mRNA-1
chr6_3 CDS 97260229 97260601 97260229 ID=Msa0967510-mRNA-1:cds;Parent=Msa0967510-mRNA-1
chr6_3 CDS 97260717 97260935 97260717 ID=Msa0967510-mRNA-1:cds;Parent=Msa0967510-mRNA-1
chr6_3 CDS 97261201 97261351 97261201 ID=Msa0967510-mRNA-1:cds;Parent=Msa0967510-mRNA-1
Gene Sequence

>Msa0967510

ATGTCTTCAGTAGATTACTTCAATCCTAATGAGAAATCGGTTTTGCAAAGAAATGTTGCAGATTTCCACCCTAGCGTTTGGGGAGATTTTTTCATTCAGTATGCTTCAGAATCTATGGAACCTGATATGAATATTGCGGCACAAATTGATAAACTAAAAAAGGAGGTTAGACAAATGCTTGTCTTAAAAACAGAGAAACCCTTGGGAAAAGTCAACTTGATTGACTCGATATGTCGTTTGGGTGTGAGCTATCACTTTGAAGAAGAAATTAGTCAAGTTTTGGAACATATTTACAAGAGTTATGTTCAAAATGGAGAAATAACTCTCAAAGACAATCTTCATTCTCTTGCTGTGCTATTTAGGGTGTTGAGACAACATGGACTTTATGTTTCACCTGACGTGTTCAACCAATTCAAGGACAAGCAAGGAAATTTCAGTGAAACATTGGTCGTAGATGTTGAGGGCATGTTAAACTTGTATGAAGCCTCACATATGATGGTTCATGGTGAAAATATTTTGGAAGAGGCTTTGGCTTTTACTTCTGGTCACCTTCAGTCCATGGCCACCCGATTGAGTCATTCTCTTGCAACACAAGTAAAACAAAGCTTAAGACAATCTCTGCGCAAAAACTTGCCTAGGCTAGTGGCAAGAAGTTACATTTCCATGTATGAGCAAGATTCATCCCATAATGAAATTCTACTTGCATTGGCAAAATTGGATTTTAATATGCTCCAAAGCCTACATCAAAAAGAATTTGGAAATATTAGCAAGTGGTGGAAGGAGTTGGATATTGCTCAAAAACTACCGTATGCACGAGATAGAGTAGTAGAATGTTGTTTTTGGGTTATGACCGTATATTTTGAGCCCCAATATTCTCAAGCAAGGAAAATGATGTCAAAATTTATCTCCATTTTATCATACATTGATGACACATATGATGCATATGGAACTATTGATGAATTAGAACTTTTTACAAAGGCCATTGAAAGGTGGGATATTAATTGCTTGGAGGATCTTCCGGATTACACGAAGTTGCTTTATAAATCACTCTTGGATGTTTATGAAGAAATAGAGCAAGAAATGAAGAAAGAAGGAAGAGAATACACCCTTGACCCCTTCTTAAAAGAAGTAAGAGTTTAG
Protein sequence

>Msa0967510

MSSVDYFNPNEKSVLQRNVADFHPSVWGDFFIQYASESMEPDMNIAAQIDKLKKEVRQMLVLKTEKPLGKVNLIDSICRLGVSYHFEEEISQVLEHIYKSYVQNGEITLKDNLHSLAVLFRVLRQHGLYVSPDVFNQFKDKQGNFSETLVVDVEGMLNLYEASHMMVHGENILEEALAFTSGHLQSMATRLSHSLATQVKQSLRQSLRKNLPRLVARSYISMYEQDSSHNEILLALAKLDFNMLQSLHQKEFGNISKWWKELDIAQKLPYARDRVVECCFWVMTVYFEPQYSQARKMMSKFISILSYIDDTYDAYGTIDELELFTKAIERWDINCLEDLPDYTKLLYKSLLDVYEEIEQEMKKEGREYTLDPFLKEVRV*