AlfalfaGEDB | Msa0968290

M. sativa cultivar zhongmu-4 / Msa0968290

KEGG: K03243
GO: -
PFAM: PF00009.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968290	sp\|G0S8G9\|IF2P_CHATD	36.638	232	107	5	14	212	520	744	2.02e-34	137
Msa0968290	sp\|G0S8G9\|IF2P_CHATD	46.789	109	56	1	235	343	860	966	1.13e-20	97.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968290	G7L1S6	75.581	344	64	4	4	346	3	327	1.19e-174	502

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0792290	Msa0968290	0.807573	4.731877e-50	-8.615850e-47
Msa0968290	Msa1079100	0.853896	1.763992e-61	-8.615850e-47
Msa0968290	Msa1124010	0.834673	2.616094e-56	-8.615850e-47
Msa0968290	Msa1169290	0.960125	3.316694e-118	-8.615850e-47
Msa0968290	Msa1177160	0.806692	7.269556e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968290	MtrunA17_Chr4g0004141	69.122	353	63	9	4	345	3	320	3.39e-161	458
Msa0968290	MtrunA17_Chr4g0000991	54.673	214	82	5	9	214	56	262	1.42e-67	218
Msa0968290	MtrunA17_Chr1g0202021	51.046	239	77	7	9	215	743	973	2.79e-65	224
Msa0968290	MtrunA17_Chr1g0202021	60.317	126	41	1	225	341	1067	1192	1.13e-38	146
Msa0968290	MtrunA17_Chr1g0201991	50.000	252	80	8	2	215	215	458	1.04e-64	219
Msa0968290	MtrunA17_Chr1g0201991	61.290	124	39	1	225	339	552	675	3.33e-39	148
Msa0968290	MtrunA17_Chr4g0000221	53.000	200	72	7	9	194	32	223	9.56e-52	178
Msa0968290	MtrunA17_Chr7g0263861	80.851	94	18	0	253	346	140	233	2.08e-46	159
Msa0968290	MtrunA17_Chr7g0263861	100.000	35	0	0	4	38	53	87	2.53e-17	80.9
Msa0968290	MtrunA17_Chr7g0264901	36.491	285	133	9	16	264	36	308	9.53e-43	152

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0968290	AT1G76810.1	46.320	231	84	7	14	212	702	924	1.46e-55	196
Msa0968290	AT1G76810.1	53.175	126	50	1	225	341	1021	1146	2.31e-32	128
Msa0968290	AT1G76720.1	43.882	237	93	7	8	212	619	847	3.29e-54	192
Msa0968290	AT1G76720.1	41.600	125	53	3	225	339	944	1058	7.96e-18	85.5
Msa0968290	AT1G76720.2	43.882	237	93	7	8	212	652	880	3.93e-54	192
Msa0968290	AT1G76720.2	41.600	125	53	3	225	339	977	1091	9.89e-18	85.1
Msa0968290	AT1G21160.1	42.373	236	98	6	8	212	488	716	2.78e-49	177
Msa0968290	AT1G21160.1	46.825	126	58	1	225	341	813	938	7.90e-29	118
Msa0968290	AT2G27700.2	41.453	234	100	7	16	219	35	261	3.54e-43	156
Msa0968290	AT2G27700.2	47.619	126	57	1	225	341	353	478	1.39e-28	116

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Find 93 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTGTTTCCTGCATCAT+AGG	0.383280	6_3:+98626254	Msa0968290:CDS
AACAAAAGAAATAACATATC+CGG	0.371811	6_3:-98626779	None:intergenic
AACAACAAAACTATTGGAGT+TGG	0.498251	6_3:+98626707	Msa0968290:CDS
AACTATGAATTGTTTGTTGG+TGG	0.503706	6_3:-98626513	None:intergenic
AAGAAGAAGATGAATGAAGA+AGG	0.510009	6_3:+98626103	Msa0968290:exon
AAGACTCAAGGGTTCAACCC+AGG	0.594325	6_3:+98627240	Msa0968290:CDS
AATAAGTGGTGAAGGAATCC+CGG	0.582764	6_3:+98626760	Msa0968290:CDS
AATCATGTCGACCTTATTCA+AGG	0.495710	6_3:-98626537	None:intergenic
AATCCTGCTCAAGATTGACT+TGG	0.550806	6_3:-98626906	None:intergenic
AATTATCACCAAACACAAGA+AGG	0.519504	6_3:+98626040	Msa0968290:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTATCACCAAACACAAGA+AGG	+	chr6_3:98626040-98626059	Msa0968290:five_prime_UTR	30.0%
ATTATCACCAAACACAAGAA+GGG	+	chr6_3:98626041-98626060	Msa0968290:five_prime_UTR	30.0%
AAGAAGAAGATGAATGAAGA+AGG	+	chr6_3:98626103-98626122	Msa0968290:exon	30.0%
ATTATGCATGATCATGATGA+TGG	+	chr6_3:98626458-98626477	Msa0968290:CDS	30.0%
TTAATAGATTGAGTGAGTCT+GGG	-	chr6_3:98626490-98626509	None:intergenic	30.0%
TTTAATAGATTGAGTGAGTC+TGG	-	chr6_3:98626491-98626510	None:intergenic	30.0%
ATTCATAGTTGCCTTGAATA+AGG	+	chr6_3:98626526-98626545	Msa0968290:CDS	30.0%
AACAACAAAACTATTGGAGT+TGG	+	chr6_3:98626707-98626726	Msa0968290:CDS	30.0%
AATTATCACCAAACACAAGA+AGG	+	chr6_3:98626040-98626059	Msa0968290:five_prime_UTR	30.0%
ATTATCACCAAACACAAGAA+GGG	+	chr6_3:98626041-98626060	Msa0968290:five_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	98626018	98627267	98626018	ID=Msa0968290;Name=Msa0968290
chr6_3	mRNA	98626018	98627267	98626018	ID=Msa0968290-mRNA-1;Parent=Msa0968290;Name=Msa0968290-mRNA-1;_AED=0.04;_eAED=0.06;_QI=94\|1\|0.5\|1\|1\|1\|2\|0\|353
chr6_3	exon	98626018	98626124	98626018	ID=Msa0968290-mRNA-1:exon:18537;Parent=Msa0968290-mRNA-1
chr6_3	exon	98626219	98627267	98626219	ID=Msa0968290-mRNA-1:exon:18538;Parent=Msa0968290-mRNA-1
chr6_3	five_prime_UTR	98626018	98626111	98626018	ID=Msa0968290-mRNA-1:five_prime_utr;Parent=Msa0968290-mRNA-1
chr6_3	CDS	98626112	98626124	98626112	ID=Msa0968290-mRNA-1:cds;Parent=Msa0968290-mRNA-1
chr6_3	CDS	98626219	98627267	98626219	ID=Msa0968290-mRNA-1:cds;Parent=Msa0968290-mRNA-1

Gene Sequence

>Msa0968290

ATGAATGAAGAAGGTGATACGGTTAAGCCGCCGCCGCCTAGTTACCGGAAACCTGTTTCCTGCATCATAGGTGATGCGTACACTGGCAAAACCATGCTTCTAGGTTGTATTCGAGGTAAAGACAATCTTCCCCATATTCCATCATCAACAACACATTTTCCTGCTGGTGATGCTGAAAGACGTATGCTGGTTGTTGATAACGCACCAATTAGGGGTTCATATCTATGTGATATTGCTATTTTGGTTGTTGACATTATGCATGATCATGATGATGGCTTACACCCAGACTCACTCAATCTATTAAAAACCACCAACAAACAATTCATAGTTGCCTTGAATAAGGTCGACATGATTCATGACTGGAAAACATGTCCCAATGCTCCATTTCGTAAAGCATTGATGCAGCAGTCTAAGGGTGTTCAAGATGACTTTCATACCAGGGTCTCTAAGGTTGTTTCTCAGTTCAAGACACACGGGCTAAATACTGATTTGTATTATAAAAACAACAAAACTATTGGAGTTGGACAAACATTCAGCATTGTGCCCACAAGTGCAATAAGTGGTGAAGGAATCCCGGATATGTTATTTCTTTTGTTTAAGTGGATCCAATACACCATTATTCACAATTTTACCTACAGTCAACAAGCTGTGCAGAGATTTATTCGTCCACCTGTGGTCAAGCCTAATGATGATTTGGACTCCAAGTCAATCTTGAGCAGGATTGACACGACTCCTGAGGGGGTTTGTGTCAATGCATGTAACTTTTACTCATTGGAAGCATTACTCAAGACTATGAAACATCCACAAGTCAATATTCCTGTTAGTGCTGTAAACATAGGTCCTGTACATAAGAAGGATGTGGAGAAGGTCAGTGCAATGCTTGAAAACAAACCAGAGTATGCTGTTGTGTTTGCGTTTCCTGTCATGGTCACCTCTGAAGCTTGGCAACTTGCACACAAATTGGGTGTCAAAATTTTCATCTCCGTTAAAATGCATCATCTATTTGACCGATTTAAACAACATGAACAACGTTTAAGACTCAAGGGTTCAACCCAGGCTTGA

Protein sequence

>Msa0968290

MNEEGDTVKPPPPSYRKPVSCIIGDAYTGKTMLLGCIRGKDNLPHIPSSTTHFPAGDAERRMLVVDNAPIRGSYLCDIAILVVDIMHDHDDGLHPDSLNLLKTTNKQFIVALNKVDMIHDWKTCPNAPFRKALMQQSKGVQDDFHTRVSKVVSQFKTHGLNTDLYYKNNKTIGVGQTFSIVPTSAISGEGIPDMLFLLFKWIQYTIIHNFTYSQQAVQRFIRPPVVKPNDDLDSKSILSRIDTTPEGVCVNACNFYSLEALLKTMKHPQVNIPVSAVNIGPVHKKDVEKVSAMLENKPEYAVVFAFPVMVTSEAWQLAHKLGVKIFISVKMHHLFDRFKQHEQRLRLKGSTQA*