AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0968920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0968920 MtrunA17_Chr7g0262051 94.737 57 3 0 1 57 1 57 1.16e-30 110
Msa0968920 MtrunA17_Chr1g0168741 58.000 50 21 0 8 57 9 58 5.59e-16 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0968920 AT5G43960.1 68.750 48 15 0 10 57 8 55 4.68e-17 73.2

Find 8 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAACTATGGCTTCGTATC+AGG 0.247670 6_4:+334058 None:intergenic
GCAGGTTGGTTCTTACTTTG+TGG 0.349830 6_4:+334202 Msa0968920:intron
CAGGTTGGTTCTTACTTTGT+GGG 0.371768 6_4:+334203 Msa0968920:intron
TAAAAGTGATAAACATGATC+AGG 0.437939 6_4:-334254 None:intergenic
AGTTCGATGATTCGTGTTGA+CGG 0.487457 6_4:+334287 Msa0968920:CDS
GGGTTCAGTTAGTGCTGCCC+AGG 0.498131 6_4:+334079 Msa0968920:CDS
GCAACTATGGCTTCGTATCA+GGG 0.517260 6_4:+334059 None:intergenic
TGAGACTGCATCTGATGTCT+TGG 0.544441 6_4:+334319 Msa0968920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTCAATTGTTTTA+GGG + chr6_4:334372-334391 Msa0968920:intron 10.0%
!! AAAGAAATAAATAAAAACCT+GGG - chr6_4:334099-334118 None:intergenic 15.0%
!! TAAAGAAATAAATAAAAACC+TGG - chr6_4:334100-334119 None:intergenic 15.0%
!!! CTTTTTTTTTCAATTGTTTT+AGG + chr6_4:334371-334390 Msa0968920:intron 15.0%
!!! ATTTTTTTTATGTGGATTCT+TGG + chr6_4:334137-334156 Msa0968920:intron 20.0%
! TAAAAGTGATAAACATGATC+AGG - chr6_4:334257-334276 None:intergenic 25.0%
TTGGATTTGTGTGAATTGAT+AGG + chr6_4:334156-334175 Msa0968920:intron 30.0%
TGGATTTGTGTGAATTGATA+GGG + chr6_4:334157-334176 Msa0968920:intron 30.0%
!! TGTTTGTTTGATTGATTTGC+AGG + chr6_4:334184-334203 Msa0968920:intron 30.0%
!! TGTTTGATTGATTTGCAGGT+TGG + chr6_4:334188-334207 Msa0968920:intron 35.0%
!! CAGGTTGGTTCTTACTTTGT+GGG + chr6_4:334203-334222 Msa0968920:intron 40.0%
AGTTCGATGATTCGTGTTGA+CGG + chr6_4:334287-334306 Msa0968920:CDS 40.0%
!! GCAGGTTGGTTCTTACTTTG+TGG + chr6_4:334202-334221 Msa0968920:intron 45.0%
TGAGACTGCATCTGATGTCT+TGG + chr6_4:334319-334338 Msa0968920:CDS 45.0%
!!! TAAATTTTATTTTTTTTATG+TGG + chr6_4:334129-334148 Msa0968920:intron 5.0%
GGGTTCAGTTAGTGCTGCCC+AGG + chr6_4:334079-334098 Msa0968920:CDS 60.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 334065 334407 334065 ID=Msa0968920;Name=Msa0968920
chr6_4 mRNA 334065 334407 334065 ID=Msa0968920-mRNA-1;Parent=Msa0968920;Name=Msa0968920-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0|0|0.33|1|1|3|0|61
chr6_4 exon 334065 334100 334065 ID=Msa0968920-mRNA-1:exon:209;Parent=Msa0968920-mRNA-1
chr6_4 exon 334206 334340 334206 ID=Msa0968920-mRNA-1:exon:210;Parent=Msa0968920-mRNA-1
chr6_4 exon 334393 334407 334393 ID=Msa0968920-mRNA-1:exon:211;Parent=Msa0968920-mRNA-1
chr6_4 CDS 334065 334100 334065 ID=Msa0968920-mRNA-1:cds;Parent=Msa0968920-mRNA-1
chr6_4 CDS 334206 334340 334206 ID=Msa0968920-mRNA-1:cds;Parent=Msa0968920-mRNA-1
chr6_4 CDS 334393 334407 334393 ID=Msa0968920-mRNA-1:cds;Parent=Msa0968920-mRNA-1
Gene Sequence

>Msa0968920

ATGGCTTCGTATCAGGGTTCAGTTAGTGCTGCCCAGGTTGGTTCTTACTTTGTGGGACAGTACTATCAAGTTCTGCGTCAACAACCTGATCATGTTTATCACTTTTATTCTGATTCGAGTTCGATGATTCGTGTTGACGGTGATTATACTGAGACTGCATCTGATGTCTTGGGTTTTAGAATTTAA

Protein sequence

>Msa0968920

MASYQGSVSAAQVGSYFVGQYYQVLRQQPDHVYHFYSDSSSMIRVDGDYTETASDVLGFRI*