Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970630 | XP_013450766.1 | 86.316 | 570 | 25 | 9 | 1 | 522 | 1 | 565 | 0.0 | 799 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970630 | A0A072U4W9 | 86.316 | 570 | 25 | 9 | 1 | 522 | 1 | 565 | 0.0 | 799 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0063780 | Msa0970630 | 0.812976 | 3.233311e-51 | -8.615850e-47 |
| Msa0193740 | Msa0970630 | 0.826942 | 2.056698e-54 | -8.615850e-47 |
| Msa0635930 | Msa0970630 | 0.807877 | 4.077976e-50 | -8.615850e-47 |
| Msa0743130 | Msa0970630 | 0.817849 | 2.664549e-52 | -8.615850e-47 |
| Msa0930830 | Msa0970630 | 0.808976 | 2.377148e-50 | -8.615850e-47 |
| Msa0970630 | Msa1193340 | -0.811826 | 5.765436e-51 | -8.615850e-47 |
| Msa0970630 | Msa1407640 | 0.802356 | 5.832765e-49 | -8.615850e-47 |
| Msa0970630 | Msa1454290 | 0.817286 | 3.568748e-52 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970630 | MtrunA17_Chr6g0449531 | 86.316 | 570 | 25 | 9 | 1 | 522 | 1 | 565 | 0.0 | 799 |
| Msa0970630 | MtrunA17_Chr6g0449551 | 65.957 | 376 | 75 | 6 | 141 | 492 | 10 | 356 | 2.00e-131 | 386 |
| Msa0970630 | MtrunA17_Chr6g0449541 | 54.412 | 68 | 27 | 1 | 62 | 125 | 45 | 112 | 5.97e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0970630 | AT3G01560.1 | 39.543 | 569 | 213 | 21 | 1 | 508 | 1 | 499 | 5.90e-69 | 230 |
| Msa0970630 | AT5G14540.1 | 53.216 | 171 | 59 | 6 | 366 | 521 | 381 | 545 | 1.63e-35 | 140 |
| Msa0970630 | AT3G01560.2 | 52.027 | 148 | 55 | 4 | 366 | 508 | 234 | 370 | 5.68e-29 | 118 |
| Msa0970630 | AT4G28300.1 | 27.932 | 469 | 252 | 18 | 91 | 519 | 74 | 496 | 3.55e-14 | 75.5 |
Find 184 sgRNAs with CRISPR-Local
Find 696 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGAGAGGTTGGTTTGGT+AGG | 0.553859 | 6_4:+2224974 | Msa0970630:CDS |
| AACAGGGGTGCATGATATCA+AGG | 0.535028 | 6_4:+2223883 | Msa0970630:CDS |
| AACATATACAGGTGCAAACA+GGG | 0.554029 | 6_4:+2223867 | Msa0970630:intron |
| AACCGAAATCAAAGATAACG+TGG | 0.656028 | 6_4:-2225344 | None:intergenic |
| AACTGTTGGGAAGGTTGAGG+AGG | 0.598242 | 6_4:-2224517 | None:intergenic |
| AAGATATTGTTGAGTTTGCA+GGG | 0.482811 | 6_4:-2224044 | None:intergenic |
| AAGCTTATGAATGAAGGTGG+TGG | 0.622241 | 6_4:+2224940 | Msa0970630:CDS |
| AATAATCATGGAATCACTGA+TGG | 0.511625 | 6_4:+2222455 | Msa0970630:CDS |
| AATGAATACGACGTCGTTTA+TGG | 0.118460 | 6_4:+2214734 | None:intergenic |
| AATGCAAGCTCAGCTACAAC+TGG | 0.504070 | 6_4:+2223919 | Msa0970630:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTTTATGGACAAACAGATAA+TGG | + | chr6_4:2214749-2214768 | Msa0970630:CDS | 30.0% | |
| TCGCTAATTCATCACAAAAA+TGG | + | chr6_4:2214876-2214895 | Msa0970630:CDS | 30.0% | |
| CTAATTCATCACAAAAATGG+CGG | + | chr6_4:2214879-2214898 | Msa0970630:CDS | 30.0% | |
| TAATCCATTCAAATGGAAGA+AGG | + | chr6_4:2215310-2215329 | Msa0970630:intron | 30.0% | |
| TTTACCTTCTTCCATTTGAA+TGG | - | chr6_4:2215317-2215336 | None:intergenic | 30.0% | |
| AAGGTAGAGATTAAGATTAG+AGG | + | chr6_4:2215374-2215393 | Msa0970630:intron | 30.0% | |
| ACATCATTCAATCAAGAAAG+AGG | + | chr6_4:2215440-2215459 | Msa0970630:intron | 30.0% | |
| TGTTTACACCGTTGAAAATT+TGG | + | chr6_4:2215536-2215555 | Msa0970630:intron | 30.0% | |
| GTTTACACCGTTGAAAATTT+GGG | + | chr6_4:2215537-2215556 | Msa0970630:intron | 30.0% | |
| TTCTCTTGATGAAACAACAA+AGG | + | chr6_4:2215751-2215770 | Msa0970630:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 2214735 | 2225380 | 2214735 | ID=Msa0970630;Name=Msa0970630 |
| chr6_4 | mRNA | 2214735 | 2225380 | 2214735 | ID=Msa0970630-mRNA-1;Parent=Msa0970630;Name=Msa0970630-mRNA-1;_AED=0.07;_eAED=0.09;_QI=0|0.25|0.2|1|0.5|0.6|5|381|522 |
| chr6_4 | exon | 2214735 | 2214900 | 2214735 | ID=Msa0970630-mRNA-1:exon:1081;Parent=Msa0970630-mRNA-1 |
| chr6_4 | exon | 2219289 | 2219316 | 2219289 | ID=Msa0970630-mRNA-1:exon:1082;Parent=Msa0970630-mRNA-1 |
| chr6_4 | exon | 2222211 | 2222394 | 2222211 | ID=Msa0970630-mRNA-1:exon:1083;Parent=Msa0970630-mRNA-1 |
| chr6_4 | exon | 2222443 | 2222511 | 2222443 | ID=Msa0970630-mRNA-1:exon:1084;Parent=Msa0970630-mRNA-1 |
| chr6_4 | exon | 2223878 | 2225380 | 2223878 | ID=Msa0970630-mRNA-1:exon:1085;Parent=Msa0970630-mRNA-1 |
| chr6_4 | CDS | 2214735 | 2214900 | 2214735 | ID=Msa0970630-mRNA-1:cds;Parent=Msa0970630-mRNA-1 |
| chr6_4 | CDS | 2219289 | 2219316 | 2219289 | ID=Msa0970630-mRNA-1:cds;Parent=Msa0970630-mRNA-1 |
| chr6_4 | CDS | 2222211 | 2222394 | 2222211 | ID=Msa0970630-mRNA-1:cds;Parent=Msa0970630-mRNA-1 |
| chr6_4 | CDS | 2222443 | 2222511 | 2222443 | ID=Msa0970630-mRNA-1:cds;Parent=Msa0970630-mRNA-1 |
| chr6_4 | CDS | 2223878 | 2224999 | 2223878 | ID=Msa0970630-mRNA-1:cds;Parent=Msa0970630-mRNA-1 |
| chr6_4 | three_prime_UTR | 2225000 | 2225380 | 2225000 | ID=Msa0970630-mRNA-1:three_prime_utr;Parent=Msa0970630-mRNA-1 |
| Gene Sequence |
>Msa0970630 ATGAATACGACGTCGTTTATGGACAAACAGATAATGGATCTAAGCCATGGAGGATCATCATCTTCCAACAACAACAACGATTTCATCGACCTTAACAAGAAGAATCGTGAACAACACGAAGAAGAAGATGAAGATGATGATTCGCTAATTCATCACAAAAATGGCGACCTTGGAATTCTGATTCCAATTCCAAATTTTCATCCTTATTTTGTGCAGAACTATGGTTCTTTGGATTCTTTTGAACCTGCAAAGGTGACTGTAGAAAAGAACCAGAGCGCTGTTGATGCTTCAATTTTGTTAGAGATTGATCGAACAATGAAGAAACATATGGACAATTTGCATCATGTTCTAGAAGGTGTTAGTGCGCGGTTAACCCAAGTATCTGTTGGAAATAATCATGGAATCACTGATGGGAAATTGAGGCTGTTGGAGAATATTCTTTCTGAGGTGCAAACAGGGGTGCATGATATCAAGGACAAACAAGATATAATGCAAGCTCAGCTACAACTGGCAAAACTACAAGTGTCTAAGACGACAGAGCAACAATCAGAAACTCAAACCCGTGCCACATCAGATCCTGTGCAGCAAACTTCATCTGTATCTGCATCTGCTCCCCTGCAAACTCAACAATATCTTCCTTCGTCTTATAATCTTCCACAATCAATCCCCGTGGTTTCTCCCCCTAATGCACCTCCTCAACCTCCCCCTCAACAAAATTTGCCACCTCCAGCTCAACTTCCAAATCAATACTCTCAGATCCCAACCCCAACTGTTCCTCAGAGAGATCCATACTTGCCACCACCTGTTCAATCTCAGGAAATTCCAAATCAGCAATACCAACTGCCTTTAACTCAGCAGCCACATCCTCAACCAGGGGCACCTCCGCATCAACAATATCAGCAGACCCCTCATCATCAATACTCTCAGCCACCGCCTCATCTACCTCCACAACAGCCACCGCATTCATCCGGAAATCCACCTCAACTGCAATCTTCCATGGGTCACCATCTAGAGGAACCACCTTATGTTCCTTCTCAGACTTATCCTCCCAATCTCCGCCAGCCACCATCTCAGCCACCCAGCGGACCTCCTCAACCTTCCCAACAGTTCTATGGAACACCGCCACAAGGATATGAACCACCATCGAGTAGACCTGGTGCGAGTTACTCTTCTGGATATGGTACATTATCTGGGCCTACTGAGCCATATCGATATGGCGGACCACCTCAATATGGTAGTAAACAGTCACAACTACCTAATGCTTCGGCGGCTTCCAGTGGTGGAAACGGTTACCCACAGCTCCCTACTGCCCGCGTACTCCCACAAGCAATACCTACTGCATCTGCAGTAAGTGGTGGTTCAGGTTCTCCTGGAGCTGGTAACAGGGTTCCTGTTGATGATATAGTCGACAAAGTTGCCACTATGGGATTCCCTAGAGATCATGTGAGGGAAACGGTTCGGAAGCTGACAGAGAATGGTCAAGCAGTTGACCTAAATACAGTGCTGGATAAGCTTATGAATGAAGGTGGTGGTGATATGCAGCAACAGAGAGGTTGGTTTGGTAGGTAA |
| Protein sequence |
>Msa0970630 MNTTSFMDKQIMDLSHGGSSSSNNNNDFIDLNKKNREQHEEEDEDDDSLIHHKNGDLGILIPIPNFHPYFVQNYGSLDSFEPAKVTVEKNQSAVDASILLEIDRTMKKHMDNLHHVLEGVSARLTQVSVGNNHGITDGKLRLLENILSEVQTGVHDIKDKQDIMQAQLQLAKLQVSKTTEQQSETQTRATSDPVQQTSSVSASAPLQTQQYLPSSYNLPQSIPVVSPPNAPPQPPPQQNLPPPAQLPNQYSQIPTPTVPQRDPYLPPPVQSQEIPNQQYQLPLTQQPHPQPGAPPHQQYQQTPHHQYSQPPPHLPPQQPPHSSGNPPQLQSSMGHHLEEPPYVPSQTYPPNLRQPPSQPPSGPPQPSQQFYGTPPQGYEPPSSRPGASYSSGYGTLSGPTEPYRYGGPPQYGSKQSQLPNASAASSGGNGYPQLPTARVLPQAIPTASAVSGGSGSPGAGNRVPVDDIVDKVATMGFPRDHVRETVRKLTENGQAVDLNTVLDKLMNEGGGDMQQQRGWFGR* |
