AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0986490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0986490 MtrunA17_Chr6g0464971 94.766 363 19 0 1 363 1 363 0.0 722
Msa0986490 MtrunA17_Chr6g0464961 89.256 363 39 0 1 363 23 385 0.0 657
Msa0986490 MtrunA17_Chr6g0465111 62.291 358 135 0 5 362 3 360 1.72e-172 484
Msa0986490 MtrunA17_Chr1g0200981 62.994 354 131 0 9 362 6 359 9.38e-166 467
Msa0986490 MtrunA17_Chr6g0465121 60.615 358 136 1 5 362 16 368 1.62e-165 467
Msa0986490 MtrunA17_Chr6g0477771 54.494 356 159 2 9 361 7 362 3.37e-141 405
Msa0986490 MtrunA17_Chr3g0110001 54.294 361 161 2 5 361 3 363 5.27e-139 399
Msa0986490 MtrunA17_Chr6g0464931 88.182 110 13 0 1 110 1 110 1.18e-56 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0986490 AT4G09670.1 59.420 345 140 0 9 353 7 351 2.55e-156 444
Msa0986490 AT1G34200.1 55.114 352 146 2 9 359 7 347 1.73e-136 393
Msa0986490 AT1G66130.1 44.134 358 191 6 5 355 3 358 2.93e-103 309

Find 111 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATGAGCCATATTATTTC+TGG 0.142070 6_4:-35035987 None:intergenic
ACCCCTGCTTCATTGTATTT+TGG 0.174819 6_4:-35038936 None:intergenic
ATTCTAAAGCTGACAATAAA+TGG 0.176493 6_4:+35039270 Msa0986490:CDS
CCAAGGATTCCAAATCTTAT+TGG 0.177750 6_4:-35036019 None:intergenic
TGTTTAATATGTAATGATAT+TGG 0.186002 6_4:+35039636 Msa0986490:three_prime_UTR
TTTAATAAAAGTACATATTA+AGG 0.217593 6_4:-35039721 None:intergenic
CATATTCATATCTGATAAAA+AGG 0.244809 6_4:-35039021 None:intergenic
ATTCCAAATCTTATTGGTTT+TGG 0.245796 6_4:-35036013 None:intergenic
TGGAGAACTTGACAAGATTT+TGG 0.253525 6_4:+35036327 Msa0986490:CDS
ACTATAACCTCACTTGGTTT+TGG 0.256553 6_4:-35039173 None:intergenic
ATATCTGATCCTAATCTCTT+TGG 0.283136 6_4:+35036433 Msa0986490:CDS
CTTGTGAAGCTTCTTTGATA+TGG 0.295852 6_4:+35038967 Msa0986490:CDS
GTTTAATATGTAATGATATT+GGG 0.299126 6_4:+35039637 Msa0986490:three_prime_UTR
AAGTATATAACAACTAAATT+TGG 0.305533 6_4:-35035860 None:intergenic
TCTCTCTTCCAGAAATAATA+TGG 0.307767 6_4:+35035979 Msa0986490:exon
ATTATTTCTGGAAGAGAGAT+TGG 0.321772 6_4:-35035975 None:intergenic
CCAATAAGATTTGGAATCCT+TGG 0.324504 6_4:+35036019 Msa0986490:CDS
CTGGTTGGTGGGATTAAGTA+TGG 0.329892 6_4:+35039245 Msa0986490:CDS
AATATGGATATAACAGCAAT+AGG 0.330557 6_4:+35039038 Msa0986490:CDS
GGTTAAGGAATTTGCTCATC+TGG 0.331077 6_4:+35039226 Msa0986490:CDS
ATCTAATGGATTGCAGTATA+TGG 0.331709 6_4:+35036360 Msa0986490:CDS
GAACCAAAACCAATAAGATT+TGG 0.339590 6_4:+35036010 Msa0986490:CDS
ATACAATGACTCTCTGTCTT+AGG 0.341904 6_4:+35039485 Msa0986490:three_prime_UTR
CCCCGTTAAGTATCCTCTAT+TGG 0.356343 6_4:+35039423 Msa0986490:three_prime_UTR
ATGTCCTTTGCTAGGAAATC+TGG 0.362493 6_4:-35038792 None:intergenic
TGAGTTTGGTGAGAGGGTTA+TGG 0.362902 6_4:-35036074 None:intergenic
TTGGAAGCTTGTGAATCTAA+TGG 0.364162 6_4:+35036346 Msa0986490:CDS
CATTATTAGTGTATTGTCAT+TGG 0.369644 6_4:+35039687 Msa0986490:three_prime_UTR
TGCATGGGAAGGGAAATTAT+TGG 0.373095 6_4:-35036152 None:intergenic
TCTCCAACATTTAAAGCAAC+TGG 0.374707 6_4:-35036310 None:intergenic
TATTCCACAAGAAGCACTTA+TGG 0.376037 6_4:+35039205 Msa0986490:CDS
CGACTAGCTTACACCTTCAC+TGG 0.390665 6_4:+35036242 Msa0986490:CDS
ACAAGAAGCACTTATGGTTA+AGG 0.393073 6_4:+35039211 Msa0986490:CDS
AAGGATTGGGTGGCTATTAG+AGG 0.393184 6_4:-35035925 None:intergenic
AACTCAACCCTCTATGCAAT+TGG 0.394302 6_4:+35036091 Msa0986490:CDS
GTGAAAGCTTCAATTGATAA+TGG 0.396592 6_4:+35039332 Msa0986490:CDS
ATAGTTGCTCCTTCCTTAAC+GGG 0.401492 6_4:-35039464 None:intergenic
TATAGTTGCTCCTTCCTTAA+CGG 0.404751 6_4:-35039465 None:intergenic
TCCAATAGAGGATACTTAAC+GGG 0.413630 6_4:-35039424 None:intergenic
AGAGCCTCATTCTCTTCATT+AGG 0.416402 6_4:-35039098 None:intergenic
AAACCAGTTGCTTTAAATGT+TGG 0.419578 6_4:+35036307 Msa0986490:CDS
GAGATGCTCTAATACTCAAT+AGG 0.424576 6_4:-35039368 None:intergenic
ATAACAGCAATAGGGGCAAA+AGG 0.425672 6_4:+35039047 Msa0986490:CDS
GAGGGTTGAGTTTGGTGAGA+GGG 0.431949 6_4:-35036080 None:intergenic
ATTGCATAGAGGGTTGAGTT+TGG 0.435320 6_4:-35036088 None:intergenic
CTCTAATACTCAATAGGAAC+AGG 0.435951 6_4:-35039362 None:intergenic
TCTCTTTGGTTCCCTTCAAT+CGG 0.438224 6_4:+35036447 Msa0986490:CDS
ATATGGATATAACAGCAATA+GGG 0.448214 6_4:+35039039 Msa0986490:CDS
CTCCAATAGAGGATACTTAA+CGG 0.448905 6_4:-35039425 None:intergenic
ATAAATGGGCAATTATTAGT+AGG 0.455056 6_4:+35039285 Msa0986490:CDS
TTGTGAAGCTTCTTTGATAT+GGG 0.455911 6_4:+35038968 Msa0986490:CDS
TGAAGGGAACCAAAGAGATT+AGG 0.466089 6_4:-35036442 None:intergenic
CTCTGTCTTAGGTAACTAAC+GGG 0.469021 6_4:+35039496 Msa0986490:three_prime_UTR
GTTCCTAGTGCATCAAGATC+TGG 0.470430 6_4:-35038825 None:intergenic
TTCTAAAGCTGACAATAAAT+GGG 0.476445 6_4:+35039271 Msa0986490:CDS
AAACCAAAATACAATGAAGC+AGG 0.489224 6_4:+35038933 Msa0986490:CDS
TATTACTCATTTAGACAAGT+AGG 0.489831 6_4:-35039600 None:intergenic
ACTTGAGTGTGGTTATACTA+AGG 0.491210 6_4:-35035944 None:intergenic
AGTATTAGAGCATCTCCAAT+GGG 0.494063 6_4:+35039375 Msa0986490:exon
GTGTGGTTATACTAAGGATT+GGG 0.494254 6_4:-35035938 None:intergenic
GGATGAATCATACCGATTGA+AGG 0.499775 6_4:-35036459 None:intergenic
AAGGAATTTGCTCATCTGGT+TGG 0.501930 6_4:+35039230 Msa0986490:CDS
AATTTGCTCATCTGGTTGGT+GGG 0.506597 6_4:+35039234 Msa0986490:CDS
CTTGATGCACTAGGAACACT+AGG 0.518180 6_4:+35038831 Msa0986490:CDS
GTAATAAAATGCGTAAAACT+TGG 0.519190 6_4:+35039535 Msa0986490:three_prime_UTR
GAATTTGCTCATCTGGTTGG+TGG 0.520943 6_4:+35039233 Msa0986490:CDS
TTGTTGAACTTTCTACTGGT+TGG 0.522425 6_4:+35039147 Msa0986490:CDS
ATTACTCATTTAGACAAGTA+GGG 0.527142 6_4:-35039599 None:intergenic
ATGTAGACAGCATCGACGTC+CGG 0.529451 6_4:-35036211 None:intergenic
GGAGTGGTCCTTGTTAAGTA+AGG 0.533286 6_4:-35039402 None:intergenic
GCTAGGAAATCTGGACTCAC+CGG 0.540515 6_4:-35038783 None:intergenic
GTAATAAAATGTGTACTACT+TGG 0.542033 6_4:+35039576 Msa0986490:three_prime_UTR
AGAGGGTTGAGTTTGGTGAG+AGG 0.543418 6_4:-35036081 None:intergenic
AACGGCTTCCAATTGCATAG+AGG 0.543759 6_4:-35036099 None:intergenic
AGTGTGGTTATACTAAGGAT+TGG 0.545209 6_4:-35035939 None:intergenic
AGAGGATACTTAACGGGGAG+TGG 0.547127 6_4:-35039418 None:intergenic
CGTTAAGTATCCTCTATTGG+AGG 0.550849 6_4:+35039426 Msa0986490:three_prime_UTR
TAACAGCAATAGGGGCAAAA+GGG 0.550871 6_4:+35039048 Msa0986490:CDS
ACGGCTTCCAATTGCATAGA+GGG 0.561772 6_4:-35036098 None:intergenic
TTAACCATAAGTGCTTCTTG+TGG 0.562009 6_4:-35039209 None:intergenic
GATGAATCATACCGATTGAA+GGG 0.566141 6_4:-35036458 None:intergenic
AGGTGTAAGCTAGTCGGAAG+TGG 0.567203 6_4:-35036235 None:intergenic
TATACCTAATGAAGAGAATG+AGG 0.567350 6_4:+35039094 Msa0986490:CDS
TAACACGAATGTCCTTTGCT+AGG 0.570558 6_4:-35038800 None:intergenic
GACGTCCGGATCATCTAAAA+CGG 0.571998 6_4:-35036197 None:intergenic
CTAGGAAATCTGGACTCACC+GGG 0.573636 6_4:-35038782 None:intergenic
CAGCAAGAACTGCCCAGTGA+AGG 0.578576 6_4:-35036255 None:intergenic
TGGAAAAGTAAACTTGAGTG+TGG 0.578718 6_4:-35035955 None:intergenic
TATGGATATAACAGCAATAG+GGG 0.579131 6_4:+35039040 Msa0986490:CDS
GAGTATTAGAGCATCTCCAA+TGG 0.579422 6_4:+35039374 Msa0986490:exon
AACCAAAATACAATGAAGCA+GGG 0.580387 6_4:+35038934 Msa0986490:CDS
AAATGAGTTCCCGTTAAGGA+AGG 0.592471 6_4:+35039455 Msa0986490:three_prime_UTR
CTAGGAACACTAGGTGACAC+TGG 0.593492 6_4:+35038840 Msa0986490:CDS
TCTCTGTCTTAGGTAACTAA+CGG 0.595482 6_4:+35039495 Msa0986490:three_prime_UTR
TGGTTGGTGGGATTAAGTAT+GGG 0.605617 6_4:+35039246 Msa0986490:CDS
AAGTATCCTCTATTGGAGGA+AGG 0.607049 6_4:+35039430 Msa0986490:three_prime_UTR
GAACACTAGGTGACACTGGT+TGG 0.608823 6_4:+35038844 Msa0986490:CDS
CAGTGAAGGTGTAAGCTAGT+CGG 0.613499 6_4:-35036241 None:intergenic
GACTAGCTTACACCTTCACT+GGG 0.618271 6_4:+35036243 Msa0986490:CDS
AAGCCAGATCTTGATGCACT+AGG 0.620052 6_4:+35038822 Msa0986490:CDS
TATTAGTAGGAAAACTCAAG+TGG 0.621897 6_4:+35039298 Msa0986490:CDS
GAGTCCAGATTTCCTAGCAA+AGG 0.627187 6_4:+35038788 Msa0986490:CDS
TAGCTATCTCTGCACAACCA+AGG 0.627873 6_4:-35036036 None:intergenic
TGGTTATACTAAGGATTGGG+TGG 0.635340 6_4:-35035935 None:intergenic
TATTAGAGCATCTCCAATGG+GGG 0.655302 6_4:+35039377 Msa0986490:exon
ACCAAAATACAATGAAGCAG+GGG 0.658206 6_4:+35038935 Msa0986490:CDS
TTGGAATCCAAAACCAAGTG+AGG 0.661767 6_4:+35039166 Msa0986490:CDS
CCAATAGAGGATACTTAACG+GGG 0.684716 6_4:-35039423 None:intergenic
ATTAGAGCATCTCCAATGGG+GGG 0.701573 6_4:+35039378 Msa0986490:exon
GTATTAGAGCATCTCCAATG+GGG 0.721305 6_4:+35039376 Msa0986490:exon
AGTATATGGATTCTACTATG+TGG 0.724513 6_4:+35036374 Msa0986490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAATTTTTGTTAAAATT+AGG + chr6_4:35036495-35036514 Msa0986490:intron 10.0%
!!! AAAACAATTTTTTTTTTACA+GGG - chr6_4:35036630-35036649 None:intergenic 10.0%
!!! AAAAACAATTTTTTTTTTAC+AGG - chr6_4:35036631-35036650 None:intergenic 10.0%
!!! AACTATTTTGTTTTTTAAAA+AGG - chr6_4:35036700-35036719 None:intergenic 10.0%
!! AAATTATCTTCATAAAATAA+AGG - chr6_4:35036909-35036928 None:intergenic 10.0%
!!! AAATAATTTTTTTTTACAAG+AGG + chr6_4:35037003-35037022 Msa0986490:intron 10.0%
!!! TAAACAAAATTTATTTTAGT+AGG + chr6_4:35037794-35037813 Msa0986490:intron 10.0%
!!! AAACAAAATTTATTTTAGTA+GGG + chr6_4:35037795-35037814 Msa0986490:intron 10.0%
!!! ATAAGTAAATTTTTTTTTGT+AGG + chr6_4:35037827-35037846 Msa0986490:intron 10.0%
!!! TAAGTAAATTTTTTTTTGTA+GGG + chr6_4:35037828-35037847 Msa0986490:intron 10.0%
!!! TAAATTTTATTGTATTTTTG+AGG + chr6_4:35037944-35037963 Msa0986490:intron 10.0%
!!! AAATTTTATTGTATTTTTGA+GGG + chr6_4:35037945-35037964 Msa0986490:intron 10.0%
!!! ATTGATTTTTAGTTTATATA+TGG + chr6_4:35037976-35037995 Msa0986490:intron 10.0%
!! AACATAATTATATCTATATA+TGG - chr6_4:35038202-35038221 None:intergenic 10.0%
!! ATAAATAAATAAAACAAATG+TGG + chr6_4:35038651-35038670 Msa0986490:intron 10.0%
!! AAGTATATAACAACTAAATT+TGG - chr6_4:35035863-35035882 None:intergenic 15.0%
!!! ATTTATTTTGGACTAAAATA+TGG + chr6_4:35036566-35036585 Msa0986490:intron 15.0%
!!! AAAAAACAAAAAATTTTGCA+GGG - chr6_4:35036806-35036825 None:intergenic 15.0%
!!! TAAAAAACAAAAAATTTTGC+AGG - chr6_4:35036805-35036824 None:intergenic 15.0%
!!! TAAAAAACAAAAAATTTTGC+AGG + chr6_4:35036805-35036824 Msa0986490:intron 15.0%
!!! AAAAAACAAAAAATTTTGCA+GGG + chr6_4:35036806-35036825 Msa0986490:intron 15.0%
!! AAACAAAACAAAAAATTACA+TGG + chr6_4:35036834-35036853 Msa0986490:intron 15.0%
!! ATAAAATAAAGGCTAAAATA+TGG - chr6_4:35036898-35036917 None:intergenic 15.0%
!!! AATCATTGTTGTAAATTTTA+AGG - chr6_4:35037042-35037061 None:intergenic 15.0%
!! TTAAAATTTACAACAATGAT+TGG + chr6_4:35037041-35037060 Msa0986490:intron 15.0%
!! TAAAATTTACAACAATGATT+GGG + chr6_4:35037042-35037061 Msa0986490:intron 15.0%
!! AATGCTATTAAAATTAAGTA+AGG - chr6_4:35037480-35037499 None:intergenic 15.0%
!!! AATTTTTGCGATATTTTTTT+CGG - chr6_4:35037636-35037655 None:intergenic 15.0%
!! AGATATAAGTAACAAAAATA+TGG - chr6_4:35037671-35037690 None:intergenic 15.0%
!! ATTTCATGTTTAATAGTTTA+AGG + chr6_4:35037766-35037785 Msa0986490:intron 15.0%
!! AAAGCTATTAAAATTAAGTA+AGG + chr6_4:35037857-35037876 Msa0986490:intron 15.0%
!!! AATTTTATTGTATTTTTGAG+GGG + chr6_4:35037946-35037965 Msa0986490:intron 15.0%
!!! TATATTATACTAACTTTTCA+AGG + chr6_4:35038122-35038141 Msa0986490:intron 15.0%
!! CCTCAAAAATAAAATAAAAA+CGG + chr6_4:35038161-35038180 Msa0986490:intron 15.0%
!! CAATAAAACACAAAATTATA+TGG + chr6_4:35038335-35038354 Msa0986490:intron 15.0%
!!! AAAAGTAGTTTATATTTTCT+TGG - chr6_4:35038365-35038384 None:intergenic 15.0%
!! ATATATATATATTTCTCTCA+TGG - chr6_4:35038427-35038446 None:intergenic 15.0%
!! CGTTAATTATATTTAATTAC+AGG + chr6_4:35038740-35038759 Msa0986490:intron 15.0%
!!! TTTATTACTTTTTTAAACGT+GGG - chr6_4:35039523-35039542 None:intergenic 15.0%
!!! TTTTATTACTTTTTTAAACG+TGG - chr6_4:35039524-35039543 None:intergenic 15.0%
!! TGTTTAATATGTAATGATAT+TGG + chr6_4:35039636-35039655 Msa0986490:three_prime_UTR 15.0%
!! GTTTAATATGTAATGATATT+GGG + chr6_4:35039637-35039656 Msa0986490:three_prime_UTR 15.0%
!!! CATATTTAAATACTTTTCAT+TGG + chr6_4:35039661-35039680 Msa0986490:three_prime_UTR 15.0%
!!! TAAAAAACAAAATAGTTTGC+AGG + chr6_4:35036703-35036722 Msa0986490:intron 20.0%
!!! TTCTTCTTATGTTTTTTTAG+AGG + chr6_4:35036939-35036958 Msa0986490:intron 20.0%
!!! ATTCTTTTATGATTTATCCT+TGG + chr6_4:35037152-35037171 Msa0986490:intron 20.0%
!!! ATTCTTTTATGATTTATCCT+TGG + chr6_4:35037152-35037171 Msa0986490:intron 20.0%
!!! CTACGAATATTATTCTTTTA+TGG + chr6_4:35037342-35037361 Msa0986490:intron 20.0%
!! AAACAAATTTAGATTGAGTA+AGG - chr6_4:35037541-35037560 None:intergenic 20.0%
!!! GTTTTTCAACAATTTTTTAC+AGG + chr6_4:35037582-35037601 Msa0986490:intron 20.0%
!! GTGTTAAGAATGTTATTAAA+AGG + chr6_4:35037606-35037625 Msa0986490:intron 20.0%
!!! ATTTTTACTCAATTATTGTG+TGG - chr6_4:35038012-35038031 None:intergenic 20.0%
!!! CCGTTTTTATTTTATTTTTG+AGG - chr6_4:35038164-35038183 None:intergenic 20.0%
!!! TATATATATATACGCGTTTT+TGG + chr6_4:35038440-35038459 Msa0986490:intron 20.0%
!! TAAACAACATCATTAACAAT+CGG - chr6_4:35038469-35038488 None:intergenic 20.0%
!! CATATTCATATCTGATAAAA+AGG - chr6_4:35039024-35039043 None:intergenic 20.0%
!!! CTTTTTATCAGATATGAATA+TGG + chr6_4:35039022-35039041 Msa0986490:CDS 20.0%
!!! ATACTCTTTTTGTTTTGAGA+AGG - chr6_4:35035895-35035914 None:intergenic 25.0%
!!! ATTCCAAATCTTATTGGTTT+TGG - chr6_4:35036016-35036035 None:intergenic 25.0%
!!! TTGTTGCTTTTTCTAATGAA+CGG - chr6_4:35036120-35036139 None:intergenic 25.0%
!!! TGAAACATTTTTTCAGTTCT+AGG - chr6_4:35036409-35036428 None:intergenic 25.0%
! CAAAAATTGAAACATCAGAA+AGG - chr6_4:35036487-35036506 None:intergenic 25.0%
! TATGCAAATCATCACAAAAA+TGG + chr6_4:35036767-35036786 Msa0986490:intron 25.0%
! ATGCAAATCATCACAAAAAT+GGG + chr6_4:35036768-35036787 Msa0986490:intron 25.0%
!!! TCTAATTTTTTGTTTCTCCA+AGG - chr6_4:35037172-35037191 None:intergenic 25.0%
!!! CTAAATTTTTTGTTTCTCCA+AGG - chr6_4:35037272-35037291 None:intergenic 25.0%
!! ATTCTTTTATGGTTTATCCT+TGG + chr6_4:35037353-35037372 Msa0986490:intron 25.0%
!! TGAAGGGTTAATTATGTTTT+TGG + chr6_4:35037422-35037441 Msa0986490:intron 25.0%
! AAGTAAGGATTAAACTTACA+AGG - chr6_4:35037465-35037484 None:intergenic 25.0%
! TTTATTGTCAAGAAACAATC+TGG - chr6_4:35038322-35038341 None:intergenic 25.0%
!!! TCAATTAGAAAAGTACTTAC+AGG + chr6_4:35038710-35038729 Msa0986490:intron 25.0%
!! ATTGCATTAGAGCAATTTTA+TGG + chr6_4:35038868-35038887 Msa0986490:CDS 25.0%
!! TTGCATTAGAGCAATTTTAT+GGG + chr6_4:35038869-35038888 Msa0986490:CDS 25.0%
!!! ATTGTATTTTGGTTTGTGTA+AGG - chr6_4:35038928-35038947 None:intergenic 25.0%
! AATATGGATATAACAGCAAT+AGG + chr6_4:35039038-35039057 Msa0986490:CDS 25.0%
! ATATGGATATAACAGCAATA+GGG + chr6_4:35039039-35039058 Msa0986490:CDS 25.0%
!! TATTTTGTTGAACTTTCTAC+TGG + chr6_4:35039143-35039162 Msa0986490:CDS 25.0%
!!! TTTTTAACTATAACCTCACT+TGG - chr6_4:35039182-35039201 None:intergenic 25.0%
!!! ATTCTAAAGCTGACAATAAA+TGG + chr6_4:35039270-35039289 Msa0986490:CDS 25.0%
!!! TTCTAAAGCTGACAATAAAT+GGG + chr6_4:35039271-35039290 Msa0986490:CDS 25.0%
! ATAAATGGGCAATTATTAGT+AGG + chr6_4:35039285-35039304 Msa0986490:CDS 25.0%
! GTAATAAAATGCGTAAAACT+TGG + chr6_4:35039535-35039554 Msa0986490:three_prime_UTR 25.0%
!! TATTACTCTTTTATGCAAGT+GGG - chr6_4:35039562-35039581 None:intergenic 25.0%
!! TTATTACTCTTTTATGCAAG+TGG - chr6_4:35039563-35039582 None:intergenic 25.0%
! GTAATAAAATGTGTACTACT+TGG + chr6_4:35039576-35039595 Msa0986490:three_prime_UTR 25.0%
! ATTACTCATTTAGACAAGTA+GGG - chr6_4:35039602-35039621 None:intergenic 25.0%
! TATTACTCATTTAGACAAGT+AGG - chr6_4:35039603-35039622 None:intergenic 25.0%
! CATTATTAGTGTATTGTCAT+TGG + chr6_4:35039687-35039706 Msa0986490:three_prime_UTR 25.0%
!!! TTTTGTTTTGAGAAGGAGAA+AGG - chr6_4:35035888-35035907 None:intergenic 30.0%
!! ATTATTTCTGGAAGAGAGAT+TGG - chr6_4:35035978-35035997 None:intergenic 30.0%
TCTCTCTTCCAGAAATAATA+TGG + chr6_4:35035979-35035998 Msa0986490:exon 30.0%
TTCATGAGCCATATTATTTC+TGG - chr6_4:35035990-35036009 None:intergenic 30.0%
GAACCAAAACCAATAAGATT+TGG + chr6_4:35036010-35036029 Msa0986490:CDS 30.0%
!! TGAACCGTATATTTTTGCAT+GGG - chr6_4:35036170-35036189 None:intergenic 30.0%
!! ATGAACCGTATATTTTTGCA+TGG - chr6_4:35036171-35036190 None:intergenic 30.0%
! AAACCAGTTGCTTTAAATGT+TGG + chr6_4:35036307-35036326 Msa0986490:CDS 30.0%
!! ATCTAATGGATTGCAGTATA+TGG + chr6_4:35036360-35036379 Msa0986490:CDS 30.0%
AGTATATGGATTCTACTATG+TGG + chr6_4:35036374-35036393 Msa0986490:CDS 30.0%
ATATCTGATCCTAATCTCTT+TGG + chr6_4:35036433-35036452 Msa0986490:CDS 30.0%
AATTGAAACATCAGAAAGGA+TGG - chr6_4:35036483-35036502 None:intergenic 30.0%
AGGAACCAAAAACTACAAAA+TGG + chr6_4:35036723-35036742 Msa0986490:intron 30.0%
GGAACCAAAAACTACAAAAT+GGG + chr6_4:35036724-35036743 Msa0986490:intron 30.0%
! TGAACCCATTTTGTAGTTTT+TGG - chr6_4:35036731-35036750 None:intergenic 30.0%
TGCAAATCATCACAAAAATG+GGG + chr6_4:35036769-35036788 Msa0986490:intron 30.0%
! CATGGACTAAAACTAAAATG+AGG + chr6_4:35036852-35036871 Msa0986490:intron 30.0%
TAAAATGAGGCATATTTGCA+GGG + chr6_4:35036865-35036884 Msa0986490:intron 30.0%
!!! CTAAGTTTTTTGTTTCTCCA+AGG - chr6_4:35037373-35037392 None:intergenic 30.0%
! TACAAGGTCGTAATTTTGTA+GGG - chr6_4:35037449-35037468 None:intergenic 30.0%
! TTACAAGGTCGTAATTTTGT+AGG - chr6_4:35037450-35037469 None:intergenic 30.0%
!!! TTTTGCGATATTTTTTTCGG+AGG - chr6_4:35037633-35037652 None:intergenic 30.0%
AAGTAAGGACTAAACTTAGA+AGG + chr6_4:35037872-35037891 Msa0986490:intron 30.0%
! TTAGAAGGTCGCAATTTTAT+AGG + chr6_4:35037887-35037906 Msa0986490:intron 30.0%
! TAGAAGGTCGCAATTTTATA+GGG + chr6_4:35037888-35037907 Msa0986490:intron 30.0%
!! TTTATTGTATTTTTGAGGGG+AGG + chr6_4:35037949-35037968 Msa0986490:intron 30.0%
!! TTATTGTATTTTTGAGGGGA+GGG + chr6_4:35037950-35037969 Msa0986490:intron 30.0%
! TTAATGATGTTGTTTAAGCC+AGG + chr6_4:35038473-35038492 Msa0986490:intron 30.0%
! ACTTTGATCCATTGACTATT+TGG + chr6_4:35038522-35038541 Msa0986490:intron 30.0%
AAACCAAAATACAATGAAGC+AGG + chr6_4:35038933-35038952 Msa0986490:CDS 30.0%
AACCAAAATACAATGAAGCA+GGG + chr6_4:35038934-35038953 Msa0986490:CDS 30.0%
TTGTGAAGCTTCTTTGATAT+GGG + chr6_4:35038968-35038987 Msa0986490:CDS 30.0%
TATGGATATAACAGCAATAG+GGG + chr6_4:35039040-35039059 Msa0986490:CDS 30.0%
TATACCTAATGAAGAGAATG+AGG + chr6_4:35039094-35039113 Msa0986490:CDS 30.0%
TATTAGTAGGAAAACTCAAG+TGG + chr6_4:35039298-35039317 Msa0986490:CDS 30.0%
GTGAAAGCTTCAATTGATAA+TGG + chr6_4:35039332-35039351 Msa0986490:CDS 30.0%
GTGTGGTTATACTAAGGATT+GGG - chr6_4:35035941-35035960 None:intergenic 35.0%
AGTGTGGTTATACTAAGGAT+TGG - chr6_4:35035942-35035961 None:intergenic 35.0%
ACTTGAGTGTGGTTATACTA+AGG - chr6_4:35035947-35035966 None:intergenic 35.0%
TGGAAAAGTAAACTTGAGTG+TGG - chr6_4:35035958-35035977 None:intergenic 35.0%
CCAAGGATTCCAAATCTTAT+TGG - chr6_4:35036022-35036041 None:intergenic 35.0%
! CCAATAAGATTTGGAATCCT+TGG + chr6_4:35036019-35036038 Msa0986490:CDS 35.0%
!! CGTATATTTTTGCATGGGAA+GGG - chr6_4:35036165-35036184 None:intergenic 35.0%
CCTTCCCATGCAAAAATATA+CGG + chr6_4:35036163-35036182 Msa0986490:CDS 35.0%
! TCTCCAACATTTAAAGCAAC+TGG - chr6_4:35036313-35036332 None:intergenic 35.0%
! TGGAGAACTTGACAAGATTT+TGG + chr6_4:35036327-35036346 Msa0986490:CDS 35.0%
TTGGAAGCTTGTGAATCTAA+TGG + chr6_4:35036346-35036365 Msa0986490:CDS 35.0%
! GATGAATCATACCGATTGAA+GGG - chr6_4:35036461-35036480 None:intergenic 35.0%
!!! TTTTTAAAATAGGCCCTTGC+AGG + chr6_4:35036672-35036691 Msa0986490:intron 35.0%
!!! TTTTAAAATAGGCCCTTGCA+GGG + chr6_4:35036673-35036692 Msa0986490:intron 35.0%
!! TTTTAAAAAGGTCCCTGCAA+GGG - chr6_4:35036688-35036707 None:intergenic 35.0%
!! TTTTTAAAAAGGTCCCTGCA+AGG - chr6_4:35036689-35036708 None:intergenic 35.0%
CTAAAATGAGGCATATTTGC+AGG + chr6_4:35036864-35036883 Msa0986490:intron 35.0%
!!! GAGTGCAGCAATTTTTTGTA+GGG - chr6_4:35037509-35037528 None:intergenic 35.0%
!!! TGAGTGCAGCAATTTTTTGT+AGG - chr6_4:35037510-35037529 None:intergenic 35.0%
ACTATGAGCCAAATAGTCAA+TGG - chr6_4:35038533-35038552 None:intergenic 35.0%
TTGGCTCATAGTTTGATTGT+TGG + chr6_4:35038541-35038560 Msa0986490:intron 35.0%
!! TTTGTTTTCGCAATTGTGCT+TGG + chr6_4:35038591-35038610 Msa0986490:intron 35.0%
TTGTGCTTGGAGAATATCAT+TGG + chr6_4:35038604-35038623 Msa0986490:intron 35.0%
! ACATGCTACATTTTCTTACC+CGG + chr6_4:35038764-35038783 Msa0986490:CDS 35.0%
!!! TGTAAGGCTTTTGCTGTTTT+GGG - chr6_4:35038912-35038931 None:intergenic 35.0%
ACCAAAATACAATGAAGCAG+GGG + chr6_4:35038935-35038954 Msa0986490:CDS 35.0%
CTTGTGAAGCTTCTTTGATA+TGG + chr6_4:35038967-35038986 Msa0986490:CDS 35.0%
TTGTTGAACTTTCTACTGGT+TGG + chr6_4:35039147-35039166 Msa0986490:CDS 35.0%
! ACTATAACCTCACTTGGTTT+TGG - chr6_4:35039176-35039195 None:intergenic 35.0%
!! TATTCCACAAGAAGCACTTA+TGG + chr6_4:35039205-35039224 Msa0986490:CDS 35.0%
TTAACCATAAGTGCTTCTTG+TGG - chr6_4:35039212-35039231 None:intergenic 35.0%
!! ACAAGAAGCACTTATGGTTA+AGG + chr6_4:35039211-35039230 Msa0986490:CDS 35.0%
CTCTAATACTCAATAGGAAC+AGG - chr6_4:35039365-35039384 None:intergenic 35.0%
GAGATGCTCTAATACTCAAT+AGG - chr6_4:35039371-35039390 None:intergenic 35.0%
! AGTATTAGAGCATCTCCAAT+GGG + chr6_4:35039375-35039394 Msa0986490:exon 35.0%
TCCAATAGAGGATACTTAAC+GGG - chr6_4:35039427-35039446 None:intergenic 35.0%
CTCCAATAGAGGATACTTAA+CGG - chr6_4:35039428-35039447 None:intergenic 35.0%
! ATTTTTCCTTCCTCCAATAG+AGG - chr6_4:35039439-35039458 None:intergenic 35.0%
TATAGTTGCTCCTTCCTTAA+CGG - chr6_4:35039468-35039487 None:intergenic 35.0%
ATACAATGACTCTCTGTCTT+AGG + chr6_4:35039485-35039504 Msa0986490:three_prime_UTR 35.0%
TCTCTGTCTTAGGTAACTAA+CGG + chr6_4:35039495-35039514 Msa0986490:three_prime_UTR 35.0%
TGGTTATACTAAGGATTGGG+TGG - chr6_4:35035938-35035957 None:intergenic 40.0%
ATTGCATAGAGGGTTGAGTT+TGG - chr6_4:35036091-35036110 None:intergenic 40.0%
AACTCAACCCTCTATGCAAT+TGG + chr6_4:35036091-35036110 Msa0986490:CDS 40.0%
TGCATGGGAAGGGAAATTAT+TGG - chr6_4:35036155-35036174 None:intergenic 40.0%
!! CCGTATATTTTTGCATGGGA+AGG - chr6_4:35036166-35036185 None:intergenic 40.0%
TGAAGGGAACCAAAGAGATT+AGG - chr6_4:35036445-35036464 None:intergenic 40.0%
! TCTCTTTGGTTCCCTTCAAT+CGG + chr6_4:35036447-35036466 Msa0986490:CDS 40.0%
! GGATGAATCATACCGATTGA+AGG - chr6_4:35036462-35036481 None:intergenic 40.0%
CAAAACGAGGCATATTTGCA+GGG - chr6_4:35036599-35036618 None:intergenic 40.0%
ATCATCACAAAAATGGGGTC+AGG + chr6_4:35036774-35036793 Msa0986490:intron 40.0%
TCATCACAAAAATGGGGTCA+GGG + chr6_4:35036775-35036794 Msa0986490:intron 40.0%
ATGTCCTTTGCTAGGAAATC+TGG - chr6_4:35038795-35038814 None:intergenic 40.0%
TAACACGAATGTCCTTTGCT+AGG - chr6_4:35038803-35038822 None:intergenic 40.0%
!!! GTGTAAGGCTTTTGCTGTTT+TGG - chr6_4:35038913-35038932 None:intergenic 40.0%
! ACCCCTGCTTCATTGTATTT+TGG - chr6_4:35038939-35038958 None:intergenic 40.0%
ATAACAGCAATAGGGGCAAA+AGG + chr6_4:35039047-35039066 Msa0986490:CDS 40.0%
TAACAGCAATAGGGGCAAAA+GGG + chr6_4:35039048-35039067 Msa0986490:CDS 40.0%
AGAGCCTCATTCTCTTCATT+AGG - chr6_4:35039101-35039120 None:intergenic 40.0%
TTGGAATCCAAAACCAAGTG+AGG + chr6_4:35039166-35039185 Msa0986490:CDS 40.0%
! GGTTAAGGAATTTGCTCATC+TGG + chr6_4:35039226-35039245 Msa0986490:CDS 40.0%
! AAGGAATTTGCTCATCTGGT+TGG + chr6_4:35039230-35039249 Msa0986490:CDS 40.0%
! AATTTGCTCATCTGGTTGGT+GGG + chr6_4:35039234-35039253 Msa0986490:CDS 40.0%
!! TGGTTGGTGGGATTAAGTAT+GGG + chr6_4:35039246-35039265 Msa0986490:CDS 40.0%
! GAGTATTAGAGCATCTCCAA+TGG + chr6_4:35039374-35039393 Msa0986490:exon 40.0%
! GTATTAGAGCATCTCCAATG+GGG + chr6_4:35039376-35039395 Msa0986490:exon 40.0%
! TATTAGAGCATCTCCAATGG+GGG + chr6_4:35039377-35039396 Msa0986490:exon 40.0%
CCAATAGAGGATACTTAACG+GGG - chr6_4:35039426-35039445 None:intergenic 40.0%
CGTTAAGTATCCTCTATTGG+AGG + chr6_4:35039426-35039445 Msa0986490:three_prime_UTR 40.0%
AAGTATCCTCTATTGGAGGA+AGG + chr6_4:35039430-35039449 Msa0986490:three_prime_UTR 40.0%
GGAAAAATGAGTTCCCGTTA+AGG + chr6_4:35039451-35039470 Msa0986490:three_prime_UTR 40.0%
AAATGAGTTCCCGTTAAGGA+AGG + chr6_4:35039455-35039474 Msa0986490:three_prime_UTR 40.0%
ATAGTTGCTCCTTCCTTAAC+GGG - chr6_4:35039467-35039486 None:intergenic 40.0%
CTCTGTCTTAGGTAACTAAC+GGG + chr6_4:35039496-35039515 Msa0986490:three_prime_UTR 40.0%
AAGGATTGGGTGGCTATTAG+AGG - chr6_4:35035928-35035947 None:intergenic 45.0%
TAGCTATCTCTGCACAACCA+AGG - chr6_4:35036039-35036058 None:intergenic 45.0%
! TGAGTTTGGTGAGAGGGTTA+TGG - chr6_4:35036077-35036096 None:intergenic 45.0%
ACGGCTTCCAATTGCATAGA+GGG - chr6_4:35036101-35036120 None:intergenic 45.0%
AACGGCTTCCAATTGCATAG+AGG - chr6_4:35036102-35036121 None:intergenic 45.0%
GACGTCCGGATCATCTAAAA+CGG - chr6_4:35036200-35036219 None:intergenic 45.0%
! CAGTGAAGGTGTAAGCTAGT+CGG - chr6_4:35036244-35036263 None:intergenic 45.0%
!! GACTAGCTTACACCTTCACT+GGG + chr6_4:35036243-35036262 Msa0986490:CDS 45.0%
CCAAAACGAGGCATATTTGC+AGG - chr6_4:35036600-35036619 None:intergenic 45.0%
! CCTGCAAATATGCCTCGTTT+TGG + chr6_4:35036597-35036616 Msa0986490:intron 45.0%
CAGGGACTAAAACCAAAACG+AGG - chr6_4:35036612-35036631 None:intergenic 45.0%
TTTGTGTCATGCTACGTTCC+TGG - chr6_4:35038494-35038513 None:intergenic 45.0%
! TTGTTGGTCTAGCTACCTCA+AGG + chr6_4:35038557-35038576 Msa0986490:intron 45.0%
! TGTTGGTCTAGCTACCTCAA+GGG + chr6_4:35038558-35038577 Msa0986490:intron 45.0%
GAGTCCAGATTTCCTAGCAA+AGG + chr6_4:35038788-35038807 Msa0986490:CDS 45.0%
AAGCCAGATCTTGATGCACT+AGG + chr6_4:35038822-35038841 Msa0986490:CDS 45.0%
GTTCCTAGTGCATCAAGATC+TGG - chr6_4:35038828-35038847 None:intergenic 45.0%
CTTGATGCACTAGGAACACT+AGG + chr6_4:35038831-35038850 Msa0986490:CDS 45.0%
! GAATTTGCTCATCTGGTTGG+TGG + chr6_4:35039233-35039252 Msa0986490:CDS 45.0%
!! CTGGTTGGTGGGATTAAGTA+TGG + chr6_4:35039245-35039264 Msa0986490:CDS 45.0%
! ATTAGAGCATCTCCAATGGG+GGG + chr6_4:35039378-35039397 Msa0986490:exon 45.0%
GGAGTGGTCCTTGTTAAGTA+AGG - chr6_4:35039405-35039424 None:intergenic 45.0%
CCCCGTTAAGTATCCTCTAT+TGG + chr6_4:35039423-35039442 Msa0986490:three_prime_UTR 45.0%
!!! ATTGTTAAAATTATTTATTT+TGG + chr6_4:35036554-35036573 Msa0986490:intron 5.0%
!!! AATTTTTTGTTTTTTAAAAT+AGG + chr6_4:35036662-35036681 Msa0986490:intron 5.0%
!!! AAATTTATTTTATTAAATGA+AGG + chr6_4:35037405-35037424 Msa0986490:intron 5.0%
!!! AATTTATTTTATTAAATGAA+GGG + chr6_4:35037406-35037425 Msa0986490:intron 5.0%
! GAGGGTTGAGTTTGGTGAGA+GGG - chr6_4:35036083-35036102 None:intergenic 50.0%
! AGAGGGTTGAGTTTGGTGAG+AGG - chr6_4:35036084-35036103 None:intergenic 50.0%
!! CGACGCCGTTTTAGATGATC+CGG + chr6_4:35036192-35036211 Msa0986490:CDS 50.0%
!! ATGTAGACAGCATCGACGTC+CGG - chr6_4:35036214-35036233 None:intergenic 50.0%
AGGTGTAAGCTAGTCGGAAG+TGG - chr6_4:35036238-35036257 None:intergenic 50.0%
!! CGACTAGCTTACACCTTCAC+TGG + chr6_4:35036242-35036261 Msa0986490:CDS 50.0%
TAGCTACCTCAAGGGATCGA+GGG + chr6_4:35038566-35038585 Msa0986490:intron 50.0%
CTAGGAAATCTGGACTCACC+GGG - chr6_4:35038785-35038804 None:intergenic 50.0%
GCTAGGAAATCTGGACTCAC+CGG - chr6_4:35038786-35038805 None:intergenic 50.0%
CTAGGAACACTAGGTGACAC+TGG + chr6_4:35038840-35038859 Msa0986490:CDS 50.0%
GAACACTAGGTGACACTGGT+TGG + chr6_4:35038844-35038863 Msa0986490:CDS 50.0%
GTTAAGTAAGGACCCCCCAT+TGG - chr6_4:35039393-35039412 None:intergenic 50.0%
TGGGGGGTCCTTACTTAACA+AGG + chr6_4:35039394-35039413 Msa0986490:three_prime_UTR 50.0%
AGAGGATACTTAACGGGGAG+TGG - chr6_4:35039421-35039440 None:intergenic 50.0%
CAGCAAGAACTGCCCAGTGA+AGG - chr6_4:35036258-35036277 None:intergenic 55.0%
CTAGCTACCTCAAGGGATCG+AGG + chr6_4:35038565-35038584 Msa0986490:intron 55.0%
AGCTACCTCAAGGGATCGAG+GGG + chr6_4:35038567-35038586 Msa0986490:intron 55.0%
CAAATCCCCTCGATCCCTTG+AGG - chr6_4:35038575-35038594 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 35035859 35039728 35035859 ID=Msa0986490;Name=Msa0986490
chr6_4 mRNA 35035859 35039728 35035859 ID=Msa0986490-mRNA-1;Parent=Msa0986490;Name=Msa0986490-mRNA-1;_AED=0.00;_eAED=0.00;_QI=139|1|1|1|1|1|2|346|363
chr6_4 exon 35035859 35036468 35035859 ID=Msa0986490-mRNA-1:exon:8977;Parent=Msa0986490-mRNA-1
chr6_4 exon 35038762 35039728 35038762 ID=Msa0986490-mRNA-1:exon:8978;Parent=Msa0986490-mRNA-1
chr6_4 five_prime_UTR 35035859 35035997 35035859 ID=Msa0986490-mRNA-1:five_prime_utr;Parent=Msa0986490-mRNA-1
chr6_4 CDS 35035998 35036468 35035998 ID=Msa0986490-mRNA-1:cds;Parent=Msa0986490-mRNA-1
chr6_4 CDS 35038762 35039382 35038762 ID=Msa0986490-mRNA-1:cds;Parent=Msa0986490-mRNA-1
chr6_4 three_prime_UTR 35039383 35039728 35039383 ID=Msa0986490-mRNA-1:three_prime_utr;Parent=Msa0986490-mRNA-1
Gene Sequence

>Msa0986490

ATGGCTCATGAAGAACCAAAACCAATAAGATTTGGAATCCTTGGTTGTGCAGAGATAGCTAGAAAAGTTTCAAGAGCCATAACCCTCTCACCAAACTCAACCCTCTATGCAATTGGAAGCCGTTCATTAGAAAAAGCAACAAAATTTGCAGCTTCCAATAATTTCCCTTCCCATGCAAAAATATACGGTTCATACGACGCCGTTTTAGATGATCCGGACGTCGATGCTGTCTACATGCCACTTCCGACTAGCTTACACCTTCACTGGGCAGTTCTTGCTGCTCAAAAGAAGAAACACTTGTTGCTTGAGAAACCAGTTGCTTTAAATGTTGGAGAACTTGACAAGATTTTGGAAGCTTGTGAATCTAATGGATTGCAGTATATGGATTCTACTATGTGGATGCATCATCCTAGAACTGAAAAAATGTTTCAGTTTATATCTGATCCTAATCTCTTTGGTTCCCTTCAATCGGTACATGCTACATTTTCTTACCCGGTGAGTCCAGATTTCCTAGCAAAGGACATTCGTGTTAAGCCAGATCTTGATGCACTAGGAACACTAGGTGACACTGGTTGGTATTGCATTAGAGCAATTTTATGGGCTGCAAATTACGAATTACCCAAAACAGCAAAAGCCTTACACAAACCAAAATACAATGAAGCAGGGGTAATTTTGTCTTGTGAAGCTTCTTTGATATGGGAAGATAACAAAGTAGCAACTTTCTTTTGTTCCTTTTTATCAGATATGAATATGGATATAACAGCAATAGGGGCAAAAGGGTCATTGCGTGTTCATGATTATGTTATACCTAATGAAGAGAATGAGGCTCTGTTTAGAACAAGTTCAAATTCTTATTTTGTTGAACTTTCTACTGGTTGGAATCCAAAACCAAGTGAGGTTATAGTTAAAAATGATATTCCACAAGAAGCACTTATGGTTAAGGAATTTGCTCATCTGGTTGGTGGGATTAAGTATGGGAATTCTAAAGCTGACAATAAATGGGCAATTATTAGTAGGAAAACTCAAGTGGTTATTGATGCTGTGAAAGCTTCAATTGATAATGGTCTTCTGCCTGTTCCTATTGAGTATTAG
Protein sequence

>Msa0986490

MAHEEPKPIRFGILGCAEIARKVSRAITLSPNSTLYAIGSRSLEKATKFAASNNFPSHAKIYGSYDAVLDDPDVDAVYMPLPTSLHLHWAVLAAQKKKHLLLEKPVALNVGELDKILEACESNGLQYMDSTMWMHHPRTEKMFQFISDPNLFGSLQSVHATFSYPVSPDFLAKDIRVKPDLDALGTLGDTGWYCIRAILWAANYELPKTAKALHKPKYNEAGVILSCEASLIWEDNKVATFFCSFLSDMNMDITAIGAKGSLRVHDYVIPNEENEALFRTSSNSYFVELSTGWNPKPSEVIVKNDIPQEALMVKEFAHLVGGIKYGNSKADNKWAIISRKTQVVIDAVKASIDNGLLPVPIEY*