AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0992050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0992050 MtrunA17_Chr1g0174011 92.708 96 7 0 1 96 1 96 5.33e-66 193
Msa0992050 MtrunA17_Chr5g0434431 92.708 96 7 0 1 96 79 174 5.57e-65 193
Msa0992050 MtrunA17_Chr6g0469071 91.837 98 8 0 1 98 521 618 5.45e-62 198
Msa0992050 MtrunA17_Chr1g0153911 92.222 90 7 0 9 98 1 90 2.31e-60 179
Msa0992050 MtrunA17_Chr4g0033251 79.592 49 9 1 51 98 1 49 1.10e-22 82.8
Msa0992050 MtrunA17_Chr2g0321271 68.750 48 15 0 51 98 1 48 1.24e-17 70.1
Msa0992050 MtrunA17_Chr2g0325841 36.170 94 59 1 6 98 1105 1198 1.38e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCATTAGGTTGCTTTCT+TGG 0.248489 6_4:-58818480 None:intergenic
GCAATAGAAAGAAGGTTAAA+TGG 0.271188 6_4:+58817650 Msa0992050:CDS
TTGCTTGATGTGAGAATATA+AGG 0.310732 6_4:-58816297 None:intergenic
GTTAACATATAGAGCTTTAG+AGG 0.328169 6_4:+58817609 Msa0992050:intron
TCATTGCTTCATTAAGATCT+TGG 0.330442 6_4:-58817488 None:intergenic
TATAAGTCATCATCAGTTAA+TGG 0.351177 6_4:-58818694 None:intergenic
TCTTGACTTGTATAAGGCTT+AGG 0.381448 6_4:+58818747 Msa0992050:CDS
AGAGGTTCATCAACTTGCTC+AGG 0.406730 6_4:+58817627 Msa0992050:CDS
GACAACTAAACTATTGTCCT+TGG 0.409668 6_4:-58817451 None:intergenic
TATCCACAATCATTGCCATT+AGG 0.411955 6_4:-58818492 None:intergenic
TATACTTACTTATATAGATA+TGG 0.415179 6_4:-58817330 None:intergenic
CTGCTAATATTACTAAGTCT+TGG 0.472781 6_4:+58818547 Msa0992050:CDS
ATATGATTTGCGAAACCAAT+GGG 0.486055 6_4:+58818714 Msa0992050:CDS
TAGCCAAGAAAGCAACCTAA+TGG 0.495668 6_4:+58818477 Msa0992050:intron
CAACCTAATGGCAATGATTG+TGG 0.497368 6_4:+58818489 Msa0992050:CDS
TCATCATCAGTTAATGGCGT+TGG 0.497730 6_4:-58818688 None:intergenic
CAAGAAAACAAGTTGCCCAT+TGG 0.504204 6_4:-58818729 None:intergenic
GTTAATAATTATTTGTCCCA+AGG 0.509187 6_4:+58817434 Msa0992050:five_prime_UTR
ACAACTAAACTATTGTCCTT+GGG 0.519949 6_4:-58817450 None:intergenic
CTGATACTCCTTTCTCGTAC+AGG 0.525727 6_4:-58816261 None:intergenic
AGCAAGTATCAACTCAGATA+AGG 0.542459 6_4:+58816315 Msa0992050:five_prime_UTR
TGCTCAGGGCAATAGAAAGA+AGG 0.550095 6_4:+58817642 Msa0992050:CDS
GAGGTTCATCAACTTGCTCA+GGG 0.558482 6_4:+58817628 Msa0992050:CDS
GAGCACTACCTGTACGAGAA+AGG 0.562293 6_4:+58816253 Msa0992050:five_prime_UTR
TATATGATTTGCGAAACCAA+TGG 0.577102 6_4:+58818713 Msa0992050:CDS
TAATATTACTAAGTCTTGGA+TGG 0.604101 6_4:+58818551 Msa0992050:CDS
GATCTTGGTGCATTGAACAA+AGG 0.604500 6_4:-58817473 None:intergenic
TCTACGTGTGCAGAAGACAC+TGG 0.621651 6_4:+58817409 Msa0992050:intron
TATTACTAAGTCTTGGATGG+AGG 0.643493 6_4:+58818554 Msa0992050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTTTTAATTACATA+GGG - chr6_4:58817085-58817104 None:intergenic 10.0%
!!! AAGTTTTTTTTTAATTACAT+AGG - chr6_4:58817086-58817105 None:intergenic 10.0%
!!! GAAAGAGAATTTTTTTTTAT+TGG + chr6_4:58816782-58816801 Msa0992050:intron 15.0%
!!! AAATTTGTTTCTTTACTTAT+TGG + chr6_4:58816875-58816894 Msa0992050:intron 15.0%
!! TATACTTACTTATATAGATA+TGG - chr6_4:58817333-58817352 None:intergenic 15.0%
!!! CTTTGATGATTTTTTTAATT+TGG + chr6_4:58817587-58817606 Msa0992050:intron 15.0%
!!! AGAAGATTGTAAATTTTTTA+TGG - chr6_4:58817926-58817945 None:intergenic 15.0%
!! TAAACTCAAATTATTTAGAA+AGG + chr6_4:58818152-58818171 Msa0992050:intron 15.0%
!! AAACTCAAATTATTTAGAAA+GGG + chr6_4:58818153-58818172 Msa0992050:intron 15.0%
!! GTATTATACAAAAGAATTAT+TGG - chr6_4:58818351-58818370 None:intergenic 15.0%
!! AACTAATCACTTTATAAAAA+TGG + chr6_4:58818443-58818462 Msa0992050:intron 15.0%
!!! GAATTTTTTGTAAATTGAGA+AGG - chr6_4:58816224-58816243 None:intergenic 20.0%
!!! TATTTTGCATATTTTCAGTT+TGG + chr6_4:58816704-58816723 Msa0992050:intron 20.0%
!! AAGATGTATCAATTTAGATT+AGG + chr6_4:58816836-58816855 Msa0992050:intron 20.0%
!!! GTTTTAGTATTATGATCTTT+TGG - chr6_4:58817249-58817268 None:intergenic 20.0%
!! TAATACTAAAACTAAAGTTC+AGG + chr6_4:58817257-58817276 Msa0992050:intron 20.0%
!! AATACTAAAACTAAAGTTCA+GGG + chr6_4:58817258-58817277 Msa0992050:intron 20.0%
!! TAATACAAGAAACAATCATA+CGG - chr6_4:58817733-58817752 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG + chr6_4:58817737-58817756 Msa0992050:intron 20.0%
!!! AGATTGTAAATTTTTTATGG+AGG - chr6_4:58817923-58817942 None:intergenic 20.0%
!!! TAAACCTATATGTTTTCAAT+TGG - chr6_4:58817986-58818005 None:intergenic 20.0%
!! AATTGAAAACATATAGGTTT+AGG + chr6_4:58817985-58818004 Msa0992050:intron 20.0%
!! ATTGAAAACATATAGGTTTA+GGG + chr6_4:58817986-58818005 Msa0992050:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG + chr6_4:58818184-58818203 Msa0992050:intron 20.0%
!! TTTTTGTAAATTGAGAAGGA+CGG - chr6_4:58816220-58816239 None:intergenic 25.0%
! ATGCAAAATAAACTTTGACA+TGG - chr6_4:58816694-58816713 None:intergenic 25.0%
! ACAATCAAAAACAAAGAGTA+AGG - chr6_4:58816917-58816936 None:intergenic 25.0%
!!! TTTAGTTTGTCTTCTTTTTC+AGG + chr6_4:58817053-58817072 Msa0992050:intron 25.0%
! ACGTAGAAGAACAATTATTA+AGG - chr6_4:58817396-58817415 None:intergenic 25.0%
! GTTAATAATTATTTGTCCCA+AGG + chr6_4:58817434-58817453 Msa0992050:five_prime_UTR 25.0%
!!! GTTATACATTTTAGTGTTGT+AGG - chr6_4:58817568-58817587 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG - chr6_4:58817709-58817728 None:intergenic 25.0%
! AATCATACGGAAAATATACA+GGG - chr6_4:58817720-58817739 None:intergenic 25.0%
! GTAGTGAAACAATATAATCT+TGG + chr6_4:58817839-58817858 Msa0992050:intron 25.0%
!! TAGCTTAATTGATTTAGTAG+TGG - chr6_4:58818058-58818077 None:intergenic 25.0%
! TAAAAAAATTCACTGAGGTA+CGG + chr6_4:58818189-58818208 Msa0992050:intron 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG - chr6_4:58818332-58818351 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG + chr6_4:58818551-58818570 Msa0992050:CDS 25.0%
! TATATTAACCTACACATTGT+AGG + chr6_4:58818654-58818673 Msa0992050:intron 25.0%
! TATAAGTCATCATCAGTTAA+TGG - chr6_4:58818697-58818716 None:intergenic 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG + chr6_4:58818741-58818760 Msa0992050:CDS 25.0%
TTGCTTGATGTGAGAATATA+AGG - chr6_4:58816300-58816319 None:intergenic 30.0%
!! TGCTTGCTATGTTAAAATGT+GGG + chr6_4:58816631-58816650 Msa0992050:intron 30.0%
GAAACAAATTTCTACCACAA+TGG - chr6_4:58816866-58816885 None:intergenic 30.0%
ATAAACAATATCAGCAGTCA+AGG - chr6_4:58816962-58816981 None:intergenic 30.0%
AAAGAAGACAAACTAAACGT+CGG - chr6_4:58817050-58817069 None:intergenic 30.0%
!! ACAAATAGATCGAAAGTGTT+TGG + chr6_4:58817192-58817211 Msa0992050:intron 30.0%
!! CAAATAGATCGAAAGTGTTT+GGG + chr6_4:58817193-58817212 Msa0992050:intron 30.0%
ACAACTAAACTATTGTCCTT+GGG - chr6_4:58817453-58817472 None:intergenic 30.0%
TCATTGCTTCATTAAGATCT+TGG - chr6_4:58817491-58817510 None:intergenic 30.0%
! GTTAACATATAGAGCTTTAG+AGG + chr6_4:58817609-58817628 Msa0992050:intron 30.0%
GCAATAGAAAGAAGGTTAAA+TGG + chr6_4:58817650-58817669 Msa0992050:CDS 30.0%
ACAAGAACCATACTTACTTT+GGG - chr6_4:58817682-58817701 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG - chr6_4:58817683-58817702 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG - chr6_4:58817721-58817740 None:intergenic 30.0%
GGAGGATAAACAAGAATAAA+AGG - chr6_4:58817905-58817924 None:intergenic 30.0%
GTAGCCAATTGAAAACATAT+AGG + chr6_4:58817979-58817998 Msa0992050:intron 30.0%
ATTATTTAGAAAGGGTAGTC+TGG + chr6_4:58818161-58818180 Msa0992050:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG + chr6_4:58818216-58818235 Msa0992050:intron 30.0%
ACAACAGTATCTATTCATGA+AGG - chr6_4:58818373-58818392 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG + chr6_4:58818547-58818566 Msa0992050:CDS 30.0%
! TATATGATTTGCGAAACCAA+TGG + chr6_4:58818713-58818732 Msa0992050:CDS 30.0%
! ATATGATTTGCGAAACCAAT+GGG + chr6_4:58818714-58818733 Msa0992050:CDS 30.0%
AGCAAGTATCAACTCAGATA+AGG + chr6_4:58816315-58816334 Msa0992050:five_prime_UTR 35.0%
! TAGTCGATTTCCTTGAACAT+GGG - chr6_4:58816389-58816408 None:intergenic 35.0%
AGCAATACTTGAAACACACA+AGG - chr6_4:58816412-58816431 None:intergenic 35.0%
ATAACTGTTCTGTGCTTTCA+CGG + chr6_4:58816569-58816588 Msa0992050:intron 35.0%
!! GTGCTTGCTATGTTAAAATG+TGG + chr6_4:58816630-58816649 Msa0992050:intron 35.0%
GATTGTCACATTGTGAATCT+CGG + chr6_4:58816931-58816950 Msa0992050:intron 35.0%
!! TTTTGGTTGATGCTGATGTT+AGG + chr6_4:58817013-58817032 Msa0992050:intron 35.0%
! TTTGGTTGATGCTGATGTTA+GGG + chr6_4:58817014-58817033 Msa0992050:intron 35.0%
AAGAAGACAAACTAAACGTC+GGG - chr6_4:58817049-58817068 None:intergenic 35.0%
TATTTGTCGAGATACAGAGA+CGG - chr6_4:58817179-58817198 None:intergenic 35.0%
!!! ATCGAAAGTGTTTGGGATTT+TGG + chr6_4:58817200-58817219 Msa0992050:intron 35.0%
GACTAAAATCCAAGCATACT+GGG - chr6_4:58817227-58817246 None:intergenic 35.0%
GACAACTAAACTATTGTCCT+TGG - chr6_4:58817454-58817473 None:intergenic 35.0%
CCATGTGAATGTACAGTAAA+AGG - chr6_4:58817820-58817839 None:intergenic 35.0%
! CCTTTTACTGTACATTCACA+TGG + chr6_4:58817817-58817836 Msa0992050:intron 35.0%
AAAGCAAATTGTTGTCACCA+AGG + chr6_4:58818010-58818029 Msa0992050:intron 35.0%
AAGCAAATTGTTGTCACCAA+GGG + chr6_4:58818011-58818030 Msa0992050:intron 35.0%
GATCTAACTCTGTTCTGTAT+GGG - chr6_4:58818251-58818270 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG - chr6_4:58818252-58818271 None:intergenic 35.0%
TACAGAACAGAGTTAGATCT+AGG + chr6_4:58818252-58818271 Msa0992050:intron 35.0%
! AGATCTAGGATTCCATTACA+TGG + chr6_4:58818266-58818285 Msa0992050:intron 35.0%
TGCTTCTGCTTATAAAAAGC+TGG + chr6_4:58818296-58818315 Msa0992050:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG - chr6_4:58818469-58818488 None:intergenic 35.0%
TATCCACAATCATTGCCATT+AGG - chr6_4:58818495-58818514 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG + chr6_4:58818554-58818573 Msa0992050:CDS 35.0%
ATTGAAGACCTACAATGTGT+AGG - chr6_4:58818665-58818684 None:intergenic 35.0%
TCTCACTTCACCATGTATGT+TGG + chr6_4:58816355-58816374 Msa0992050:intron 40.0%
! GTAGTCGATTTCCTTGAACA+TGG - chr6_4:58816390-58816409 None:intergenic 40.0%
TTTGTAAGCACAACAGTGGT+GGG - chr6_4:58816509-58816528 None:intergenic 40.0%
ACACTTTGTAAGCACAACAG+TGG - chr6_4:58816513-58816532 None:intergenic 40.0%
TAACTGTTCTGTGCTTTCAC+GGG + chr6_4:58816570-58816589 Msa0992050:intron 40.0%
TTAGATTAGGAGCTCCATTG+TGG + chr6_4:58816849-58816868 Msa0992050:intron 40.0%
! TCCAGCTCAATTCTCTCTTT+TGG + chr6_4:58816996-58817015 Msa0992050:intron 40.0%
ACCAAAAGAGAGAATTGAGC+TGG - chr6_4:58817000-58817019 None:intergenic 40.0%
! TTGGTTGATGCTGATGTTAG+GGG + chr6_4:58817015-58817034 Msa0992050:intron 40.0%
AAAACTGTCACTGAGTATGG+TGG - chr6_4:58817111-58817130 None:intergenic 40.0%
GCAAAAACTGTCACTGAGTA+TGG - chr6_4:58817114-58817133 None:intergenic 40.0%
GGACTAAAATCCAAGCATAC+TGG - chr6_4:58817228-58817247 None:intergenic 40.0%
TTCAGGGACACATACAAACT+AGG + chr6_4:58817274-58817293 Msa0992050:intron 40.0%
! GATCTTGGTGCATTGAACAA+AGG - chr6_4:58817476-58817495 None:intergenic 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG + chr6_4:58817672-58817691 Msa0992050:intron 40.0%
AAGCATGTGAGTCCATGTAA+TGG - chr6_4:58818281-58818300 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG + chr6_4:58818477-58818496 Msa0992050:intron 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG - chr6_4:58818483-58818502 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG + chr6_4:58818489-58818508 Msa0992050:CDS 40.0%
TCATCATCAGTTAATGGCGT+TGG - chr6_4:58818691-58818710 None:intergenic 40.0%
CAAGAAAACAAGTTGCCCAT+TGG - chr6_4:58818732-58818751 None:intergenic 40.0%
CTGATACTCCTTTCTCGTAC+AGG - chr6_4:58816264-58816283 None:intergenic 45.0%
GGTAACTGCTCCAACATACA+TGG - chr6_4:58816368-58816387 None:intergenic 45.0%
! AGTGGTGGGACTCAAAGAAA+TGG - chr6_4:58816495-58816514 None:intergenic 45.0%
CTTTGTAAGCACAACAGTGG+TGG - chr6_4:58816510-58816529 None:intergenic 45.0%
AGATACGTATCCACCAGTCA+AGG + chr6_4:58816745-58816764 Msa0992050:intron 45.0%
! GATTTTGGACCCAGTATGCT+TGG + chr6_4:58817215-58817234 Msa0992050:intron 45.0%
! AGAGGTTCATCAACTTGCTC+AGG + chr6_4:58817627-58817646 Msa0992050:CDS 45.0%
! GAGGTTCATCAACTTGCTCA+GGG + chr6_4:58817628-58817647 Msa0992050:CDS 45.0%
TGCTCAGGGCAATAGAAAGA+AGG + chr6_4:58817642-58817661 Msa0992050:CDS 45.0%
CTAAGAAACGAACACACCCT+TGG - chr6_4:58818030-58818049 None:intergenic 45.0%
!! ATAAGTAATTAATATTAATA+AGG - chr6_4:58818637-58818656 None:intergenic 5.0%
GAGCACTACCTGTACGAGAA+AGG + chr6_4:58816253-58816272 Msa0992050:five_prime_UTR 50.0%
GGAGCAGTTACCCATGTTCA+AGG + chr6_4:58816376-58816395 Msa0992050:intron 50.0%
AAGTCGTGAACCTTGACTGG+TGG - chr6_4:58816758-58816777 None:intergenic 50.0%
TCGAAGTCGTGAACCTTGAC+TGG - chr6_4:58816761-58816780 None:intergenic 50.0%
TTGTCGAGATACAGAGACGG+AGG - chr6_4:58817176-58817195 None:intergenic 50.0%
TCTACGTGTGCAGAAGACAC+TGG + chr6_4:58817409-58817428 Msa0992050:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 58816182 58818768 58816182 ID=Msa0992050;Name=Msa0992050
chr6_4 mRNA 58816182 58818768 58816182 ID=Msa0992050-mRNA-1;Parent=Msa0992050;Name=Msa0992050-mRNA-1;_AED=0.18;_eAED=0.18;_QI=257|0.66|0.57|0.71|0.5|0.42|7|0|98
chr6_4 exon 58816182 58816336 58816182 ID=Msa0992050-mRNA-1:exon:11623;Parent=Msa0992050-mRNA-1
chr6_4 exon 58817075 58817087 58817075 ID=Msa0992050-mRNA-1:exon:11624;Parent=Msa0992050-mRNA-1
chr6_4 exon 58817316 58817343 58817316 ID=Msa0992050-mRNA-1:exon:11625;Parent=Msa0992050-mRNA-1
chr6_4 exon 58817422 58817526 58817422 ID=Msa0992050-mRNA-1:exon:11626;Parent=Msa0992050-mRNA-1
chr6_4 exon 58817621 58817684 58817621 ID=Msa0992050-mRNA-1:exon:11627;Parent=Msa0992050-mRNA-1
chr6_4 exon 58818480 58818575 58818480 ID=Msa0992050-mRNA-1:exon:11628;Parent=Msa0992050-mRNA-1
chr6_4 exon 58818676 58818768 58818676 ID=Msa0992050-mRNA-1:exon:11629;Parent=Msa0992050-mRNA-1
chr6_4 five_prime_UTR 58816182 58816336 58816182 ID=Msa0992050-mRNA-1:five_prime_utr;Parent=Msa0992050-mRNA-1
chr6_4 five_prime_UTR 58817075 58817087 58817075 ID=Msa0992050-mRNA-1:five_prime_utr;Parent=Msa0992050-mRNA-1
chr6_4 five_prime_UTR 58817316 58817343 58817316 ID=Msa0992050-mRNA-1:five_prime_utr;Parent=Msa0992050-mRNA-1
chr6_4 five_prime_UTR 58817422 58817482 58817422 ID=Msa0992050-mRNA-1:five_prime_utr;Parent=Msa0992050-mRNA-1
chr6_4 CDS 58817483 58817526 58817483 ID=Msa0992050-mRNA-1:cds;Parent=Msa0992050-mRNA-1
chr6_4 CDS 58817621 58817684 58817621 ID=Msa0992050-mRNA-1:cds;Parent=Msa0992050-mRNA-1
chr6_4 CDS 58818480 58818575 58818480 ID=Msa0992050-mRNA-1:cds;Parent=Msa0992050-mRNA-1
chr6_4 CDS 58818676 58818768 58818676 ID=Msa0992050-mRNA-1:cds;Parent=Msa0992050-mRNA-1
Gene Sequence

>Msa0992050

ATGCACCAAGATCTTAATGAAGCAATGATTAAGATTGTTTCAAAAGCTTTAGAGGTTCATCAACTTGCTCAGGGCAATAGAAAGAAGGTTAAATGGTTTCGTCCCAAACCAAGAAAGCAACCTAATGGCAATGATTGTGGATACTATGTGATGAAGAATATGCTAGATATCGTCTCTGCTAATATTACTAAGTCTTGGATGGAGGTCTTCAATGATCCAACGCCATTAACTGATGATGACTTATATGATTTGCGAAACCAATGGGCAACTTGTTTTCTTGACTTGTATAAGGCTTAG

Protein sequence

>Msa0992050

MHQDLNEAMIKIVSKALEVHQLAQGNRKKVKWFRPKPRKQPNGNDCGYYVMKNMLDIVSANITKSWMEVFNDPTPLTDDDLYDLRNQWATCFLDLYKA*