AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0855760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0855760 MtrunA17_Chr5g0434431 91.597 119 10 0 22 140 56 174 5.01e-80 233
Msa0855760 MtrunA17_Chr6g0469071 90.909 121 11 0 22 142 498 618 3.97e-77 239
Msa0855760 MtrunA17_Chr1g0174011 93.750 96 6 0 45 140 1 96 3.06e-65 193
Msa0855760 MtrunA17_Chr1g0153911 93.333 90 6 0 53 142 1 90 1.10e-59 179
Msa0855760 MtrunA17_Chr2g0325841 40.336 119 70 1 25 142 1080 1198 1.50e-27 106
Msa0855760 MtrunA17_Chr4g0033251 81.633 49 8 1 95 142 1 49 5.27e-22 82.8
Msa0855760 MtrunA17_Chr2g0321271 66.667 48 16 0 95 142 1 48 8.46e-17 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCATTAGGTTGCTTTCT+TGG 0.248489 5_4:-49678827 None:intergenic
GCAATAGAAAGAAGGTTAAA+TGG 0.271188 5_4:+49678376 Msa0855760:CDS
GTTAACATATAGAGCTTTAG+AGG 0.328169 5_4:+49678335 Msa0855760:intron
TCATTGCTTCATTAAGATCT+TGG 0.330442 5_4:-49678214 None:intergenic
TTAAGTGAAGAGTTTGATAA+TGG 0.345649 5_4:+49678007 Msa0855760:CDS
TATAAGTCATCATCAGTTAA+TGG 0.351177 5_4:-49679041 None:intergenic
TCTTGACTTGTATAAGGCTT+AGG 0.381448 5_4:+49679094 Msa0855760:CDS
AGAGGTTCATCAACTTGCTC+AGG 0.406730 5_4:+49678353 Msa0855760:CDS
GACAACTAAACTATTGTCCT+TGG 0.409668 5_4:-49678177 None:intergenic
TATCCACAATCATTGCCATT+AGG 0.411955 5_4:-49678839 None:intergenic
CTGCTAATATTACTAAGTCT+TGG 0.472781 5_4:+49678894 Msa0855760:CDS
ATATGATTTGCGAAACCAAT+GGG 0.486055 5_4:+49679061 Msa0855760:CDS
TAGCCAAGAAAGCAACCTAA+TGG 0.495668 5_4:+49678824 Msa0855760:intron
CAACCTAATGGCAATGATTG+TGG 0.497368 5_4:+49678836 Msa0855760:CDS
TCATCATCAGTTAATGGCGT+TGG 0.497730 5_4:-49679035 None:intergenic
CAAGAAAACAAGTTGCCCAT+TGG 0.504204 5_4:-49679076 None:intergenic
GTTAATAATTATTTGTCCCA+AGG 0.509187 5_4:+49678160 Msa0855760:CDS
ACAACTAAACTATTGTCCTT+GGG 0.519949 5_4:-49678176 None:intergenic
GCGTCATTATGGATCGTAGT+TGG 0.549424 5_4:+49677969 None:intergenic
TGCTCAGGGCAATAGAAAGA+AGG 0.550095 5_4:+49678368 Msa0855760:CDS
GAGGTTCATCAACTTGCTCA+GGG 0.558482 5_4:+49678354 Msa0855760:CDS
TATATGATTTGCGAAACCAA+TGG 0.577102 5_4:+49679060 Msa0855760:CDS
TAATATTACTAAGTCTTGGA+TGG 0.604101 5_4:+49678898 Msa0855760:CDS
GATCTTGGTGCATTGAACAA+AGG 0.604500 5_4:-49678199 None:intergenic
TCTACGTGTGCAGAAGACAC+TGG 0.621651 5_4:+49678135 Msa0855760:intron
TATTACTAAGTCTTGGATGG+AGG 0.643493 5_4:+49678901 Msa0855760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGATGATTTTTTTAATT+TGG + chr5_4:49678313-49678332 Msa0855760:intron 15.0%
!! TAAACTCAAATTATTTAGAA+AGG + chr5_4:49678506-49678525 Msa0855760:intron 15.0%
!! AAACTCAAATTATTTAGAAA+GGG + chr5_4:49678507-49678526 Msa0855760:intron 15.0%
!! GTATTATACAAAAGAATTAT+TGG - chr5_4:49678700-49678719 None:intergenic 15.0%
!! AACTAATCACTTTATAAAAA+TGG + chr5_4:49678790-49678809 Msa0855760:intron 15.0%
!!! CTTTGATGATTTTTTTAATT+TGG + chr5_4:49678313-49678332 Msa0855760:intron 15.0%
!! TAAACTCAAATTATTTAGAA+AGG + chr5_4:49678506-49678525 Msa0855760:intron 15.0%
!! AAACTCAAATTATTTAGAAA+GGG + chr5_4:49678507-49678526 Msa0855760:intron 15.0%
!! GTATTATACAAAAGAATTAT+TGG - chr5_4:49678700-49678719 None:intergenic 15.0%
!! AACTAATCACTTTATAAAAA+TGG + chr5_4:49678790-49678809 Msa0855760:intron 15.0%
!! TAATACAAGAAACAATCATA+CGG - chr5_4:49678459-49678478 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG + chr5_4:49678463-49678482 Msa0855760:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG + chr5_4:49678538-49678557 Msa0855760:intron 20.0%
!! TAATACAAGAAACAATCATA+CGG - chr5_4:49678459-49678478 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG + chr5_4:49678463-49678482 Msa0855760:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG + chr5_4:49678538-49678557 Msa0855760:intron 20.0%
! TTAAGTGAAGAGTTTGATAA+TGG + chr5_4:49678007-49678026 Msa0855760:CDS 25.0%
! ACGTAGAAGAACAATTATTA+AGG - chr5_4:49678122-49678141 None:intergenic 25.0%
! GTTAATAATTATTTGTCCCA+AGG + chr5_4:49678160-49678179 Msa0855760:CDS 25.0%
!!! GTTATACATTTTAGTGTTGT+AGG - chr5_4:49678294-49678313 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG - chr5_4:49678435-49678454 None:intergenic 25.0%
! AATCATACGGAAAATATACA+GGG - chr5_4:49678446-49678465 None:intergenic 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG - chr5_4:49678681-49678700 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG + chr5_4:49678898-49678917 Msa0855760:CDS 25.0%
! TATATTAACCTACACATTGT+AGG + chr5_4:49679001-49679020 Msa0855760:intron 25.0%
! TATAAGTCATCATCAGTTAA+TGG - chr5_4:49679044-49679063 None:intergenic 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG + chr5_4:49679088-49679107 Msa0855760:CDS 25.0%
! TTAAGTGAAGAGTTTGATAA+TGG + chr5_4:49678007-49678026 Msa0855760:CDS 25.0%
! ACGTAGAAGAACAATTATTA+AGG - chr5_4:49678122-49678141 None:intergenic 25.0%
! GTTAATAATTATTTGTCCCA+AGG + chr5_4:49678160-49678179 Msa0855760:CDS 25.0%
!!! GTTATACATTTTAGTGTTGT+AGG - chr5_4:49678294-49678313 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG - chr5_4:49678435-49678454 None:intergenic 25.0%
! AATCATACGGAAAATATACA+GGG - chr5_4:49678446-49678465 None:intergenic 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG - chr5_4:49678681-49678700 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG + chr5_4:49678898-49678917 Msa0855760:CDS 25.0%
! TATATTAACCTACACATTGT+AGG + chr5_4:49679001-49679020 Msa0855760:intron 25.0%
! TATAAGTCATCATCAGTTAA+TGG - chr5_4:49679044-49679063 None:intergenic 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG + chr5_4:49679088-49679107 Msa0855760:CDS 25.0%
ACAACTAAACTATTGTCCTT+GGG - chr5_4:49678179-49678198 None:intergenic 30.0%
TCATTGCTTCATTAAGATCT+TGG - chr5_4:49678217-49678236 None:intergenic 30.0%
! GTTAACATATAGAGCTTTAG+AGG + chr5_4:49678335-49678354 Msa0855760:intron 30.0%
GCAATAGAAAGAAGGTTAAA+TGG + chr5_4:49678376-49678395 Msa0855760:CDS 30.0%
ACAAGAACCATACTTACTTT+GGG - chr5_4:49678408-49678427 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG - chr5_4:49678409-49678428 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG - chr5_4:49678447-49678466 None:intergenic 30.0%
ATTATTTAGAAAGGGTAGTC+TGG + chr5_4:49678515-49678534 Msa0855760:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG + chr5_4:49678565-49678584 Msa0855760:intron 30.0%
ACAACAGTATCTATTCATGA+AGG - chr5_4:49678722-49678741 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG + chr5_4:49678894-49678913 Msa0855760:CDS 30.0%
! TATATGATTTGCGAAACCAA+TGG + chr5_4:49679060-49679079 Msa0855760:CDS 30.0%
! ATATGATTTGCGAAACCAAT+GGG + chr5_4:49679061-49679080 Msa0855760:CDS 30.0%
ACAACTAAACTATTGTCCTT+GGG - chr5_4:49678179-49678198 None:intergenic 30.0%
TCATTGCTTCATTAAGATCT+TGG - chr5_4:49678217-49678236 None:intergenic 30.0%
! GTTAACATATAGAGCTTTAG+AGG + chr5_4:49678335-49678354 Msa0855760:intron 30.0%
GCAATAGAAAGAAGGTTAAA+TGG + chr5_4:49678376-49678395 Msa0855760:CDS 30.0%
ACAAGAACCATACTTACTTT+GGG - chr5_4:49678408-49678427 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG - chr5_4:49678409-49678428 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG - chr5_4:49678447-49678466 None:intergenic 30.0%
ATTATTTAGAAAGGGTAGTC+TGG + chr5_4:49678515-49678534 Msa0855760:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG + chr5_4:49678565-49678584 Msa0855760:intron 30.0%
ACAACAGTATCTATTCATGA+AGG - chr5_4:49678722-49678741 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG + chr5_4:49678894-49678913 Msa0855760:CDS 30.0%
! TATATGATTTGCGAAACCAA+TGG + chr5_4:49679060-49679079 Msa0855760:CDS 30.0%
! ATATGATTTGCGAAACCAAT+GGG + chr5_4:49679061-49679080 Msa0855760:CDS 30.0%
GACAACTAAACTATTGTCCT+TGG - chr5_4:49678180-49678199 None:intergenic 35.0%
GATCTAACTCTGTTCTGTAT+GGG - chr5_4:49678600-49678619 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG - chr5_4:49678601-49678620 None:intergenic 35.0%
TACAGAACAGAGTTAGATCT+AGG + chr5_4:49678601-49678620 Msa0855760:intron 35.0%
! AGATCTAGGATTCCATTACA+TGG + chr5_4:49678615-49678634 Msa0855760:intron 35.0%
TGCTTCTGCTTATAAAAAGC+TGG + chr5_4:49678645-49678664 Msa0855760:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG - chr5_4:49678816-49678835 None:intergenic 35.0%
TATCCACAATCATTGCCATT+AGG - chr5_4:49678842-49678861 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG + chr5_4:49678901-49678920 Msa0855760:CDS 35.0%
ATTGAAGACCTACAATGTGT+AGG - chr5_4:49679012-49679031 None:intergenic 35.0%
GACAACTAAACTATTGTCCT+TGG - chr5_4:49678180-49678199 None:intergenic 35.0%
GATCTAACTCTGTTCTGTAT+GGG - chr5_4:49678600-49678619 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG - chr5_4:49678601-49678620 None:intergenic 35.0%
TACAGAACAGAGTTAGATCT+AGG + chr5_4:49678601-49678620 Msa0855760:intron 35.0%
! AGATCTAGGATTCCATTACA+TGG + chr5_4:49678615-49678634 Msa0855760:intron 35.0%
TGCTTCTGCTTATAAAAAGC+TGG + chr5_4:49678645-49678664 Msa0855760:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG - chr5_4:49678816-49678835 None:intergenic 35.0%
TATCCACAATCATTGCCATT+AGG - chr5_4:49678842-49678861 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG + chr5_4:49678901-49678920 Msa0855760:CDS 35.0%
ATTGAAGACCTACAATGTGT+AGG - chr5_4:49679012-49679031 None:intergenic 35.0%
! GATCTTGGTGCATTGAACAA+AGG - chr5_4:49678202-49678221 None:intergenic 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG + chr5_4:49678398-49678417 Msa0855760:intron 40.0%
AAGCATGTGAGTCCATGTAA+TGG - chr5_4:49678630-49678649 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG + chr5_4:49678824-49678843 Msa0855760:intron 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG - chr5_4:49678830-49678849 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG + chr5_4:49678836-49678855 Msa0855760:CDS 40.0%
TCATCATCAGTTAATGGCGT+TGG - chr5_4:49679038-49679057 None:intergenic 40.0%
CAAGAAAACAAGTTGCCCAT+TGG - chr5_4:49679079-49679098 None:intergenic 40.0%
! GATCTTGGTGCATTGAACAA+AGG - chr5_4:49678202-49678221 None:intergenic 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG + chr5_4:49678398-49678417 Msa0855760:intron 40.0%
AAGCATGTGAGTCCATGTAA+TGG - chr5_4:49678630-49678649 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG + chr5_4:49678824-49678843 Msa0855760:intron 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG - chr5_4:49678830-49678849 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG + chr5_4:49678836-49678855 Msa0855760:CDS 40.0%
TCATCATCAGTTAATGGCGT+TGG - chr5_4:49679038-49679057 None:intergenic 40.0%
CAAGAAAACAAGTTGCCCAT+TGG - chr5_4:49679079-49679098 None:intergenic 40.0%
! AGAGGTTCATCAACTTGCTC+AGG + chr5_4:49678353-49678372 Msa0855760:CDS 45.0%
! GAGGTTCATCAACTTGCTCA+GGG + chr5_4:49678354-49678373 Msa0855760:CDS 45.0%
TGCTCAGGGCAATAGAAAGA+AGG + chr5_4:49678368-49678387 Msa0855760:CDS 45.0%
! AGAGGTTCATCAACTTGCTC+AGG + chr5_4:49678353-49678372 Msa0855760:CDS 45.0%
! GAGGTTCATCAACTTGCTCA+GGG + chr5_4:49678354-49678373 Msa0855760:CDS 45.0%
TGCTCAGGGCAATAGAAAGA+AGG + chr5_4:49678368-49678387 Msa0855760:CDS 45.0%
!! ATAAGTAATTAATATTAATA+AGG - chr5_4:49678984-49679003 None:intergenic 5.0%
!! ATAAGTAATTAATATTAATA+AGG - chr5_4:49678984-49679003 None:intergenic 5.0%
TCTACGTGTGCAGAAGACAC+TGG + chr5_4:49678135-49678154 Msa0855760:intron 50.0%
TCTACGTGTGCAGAAGACAC+TGG + chr5_4:49678135-49678154 Msa0855760:intron 50.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 49677977 49679115 49677977 ID=Msa0855760;Name=Msa0855760
chr5_4 mRNA 49677977 49679115 49677977 ID=Msa0855760-mRNA-1;Parent=Msa0855760;Name=Msa0855760-mRNA-1;_AED=0.60;_eAED=0.60;_QI=0|0.25|0.2|0.4|1|1|5|0|142
chr5_4 exon 49677977 49678047 49677977 ID=Msa0855760-mRNA-1:exon:15562;Parent=Msa0855760-mRNA-1
chr5_4 exon 49678148 49678252 49678148 ID=Msa0855760-mRNA-1:exon:15563;Parent=Msa0855760-mRNA-1
chr5_4 exon 49678347 49678410 49678347 ID=Msa0855760-mRNA-1:exon:15564;Parent=Msa0855760-mRNA-1
chr5_4 exon 49678827 49678922 49678827 ID=Msa0855760-mRNA-1:exon:15565;Parent=Msa0855760-mRNA-1
chr5_4 exon 49679023 49679115 49679023 ID=Msa0855760-mRNA-1:exon:15566;Parent=Msa0855760-mRNA-1
chr5_4 CDS 49677977 49678047 49677977 ID=Msa0855760-mRNA-1:cds;Parent=Msa0855760-mRNA-1
chr5_4 CDS 49678148 49678252 49678148 ID=Msa0855760-mRNA-1:cds;Parent=Msa0855760-mRNA-1
chr5_4 CDS 49678347 49678410 49678347 ID=Msa0855760-mRNA-1:cds;Parent=Msa0855760-mRNA-1
chr5_4 CDS 49678827 49678922 49678827 ID=Msa0855760-mRNA-1:cds;Parent=Msa0855760-mRNA-1
chr5_4 CDS 49679023 49679115 49679023 ID=Msa0855760-mRNA-1:cds;Parent=Msa0855760-mRNA-1
Gene Sequence

>Msa0855760

ATGGATCGTAGTTGGATGAGAGCTAATCGATTAAGTGAAGAGTTTGATAATGGAGTTGAAGAATTTCTAGAAAGACACTGGCAGTTAATAATTATTTGTCCCAAGGACAATAGTTTAGTTGTCCTTTGTTCAATGCACCAAGATCTTAATGAAGCAATGATTAAGATTGTTTCAAAAGCTTTAGAGGTTCATCAACTTGCTCAGGGCAATAGAAAGAAGGTTAAATGGTTTCGTCCCAAACCAAGAAAGCAACCTAATGGCAATGATTGTGGATACTATGTGATGAAGAATATGCTAGATATCATCTCTGCTAATATTACTAAGTCTTGGATGGAGGTCTTCAATGATCCAACGCCATTAACTGATGATGACTTATATGATTTGCGAAACCAATGGGCAACTTGTTTTCTTGACTTGTATAAGGCTTAG

Protein sequence

>Msa0855760

MDRSWMRANRLSEEFDNGVEEFLERHWQLIIICPKDNSLVVLCSMHQDLNEAMIKIVSKALEVHQLAQGNRKKVKWFRPKPRKQPNGNDCGYYVMKNMLDIISANITKSWMEVFNDPTPLTDDDLYDLRNQWATCFLDLYKA*