AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0993780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0993780 MtrunA17_Chr1g0174011 92.708 96 7 0 1 96 1 96 5.33e-66 193
Msa0993780 MtrunA17_Chr5g0434431 92.708 96 7 0 1 96 79 174 5.57e-65 193
Msa0993780 MtrunA17_Chr6g0469071 91.837 98 8 0 1 98 521 618 5.45e-62 198
Msa0993780 MtrunA17_Chr1g0153911 92.222 90 7 0 9 98 1 90 2.31e-60 179
Msa0993780 MtrunA17_Chr4g0033251 79.592 49 9 1 51 98 1 49 1.10e-22 82.8
Msa0993780 MtrunA17_Chr2g0321271 68.750 48 15 0 51 98 1 48 1.24e-17 70.1
Msa0993780 MtrunA17_Chr2g0325841 36.170 94 59 1 6 98 1105 1198 1.38e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCATTAGGTTGCTTTCT+TGG 0.248489 6_4:-66926106 None:intergenic
GCAATAGAAAGAAGGTTAAA+TGG 0.271188 6_4:+66925276 Msa0993780:CDS
TTGCTTGATGTGAGAATATA+AGG 0.310732 6_4:-66923923 None:intergenic
GTTAACATATAGAGCTTTAG+AGG 0.328169 6_4:+66925235 Msa0993780:intron
TCATTGCTTCATTAAGATCT+TGG 0.330442 6_4:-66925114 None:intergenic
TATAAGTCATCATCAGTTAA+TGG 0.351177 6_4:-66926320 None:intergenic
TCTTGACTTGTATAAGGCTT+AGG 0.381448 6_4:+66926373 Msa0993780:CDS
AGAGGTTCATCAACTTGCTC+AGG 0.406730 6_4:+66925253 Msa0993780:CDS
GACAACTAAACTATTGTCCT+TGG 0.409668 6_4:-66925077 None:intergenic
TATCCACAATCATTGCCATT+AGG 0.411955 6_4:-66926118 None:intergenic
TATACTTACTTATATAGATA+TGG 0.415179 6_4:-66924956 None:intergenic
CTGCTAATATTACTAAGTCT+TGG 0.472781 6_4:+66926173 Msa0993780:CDS
ATATGATTTGCGAAACCAAT+GGG 0.486055 6_4:+66926340 Msa0993780:CDS
TAGCCAAGAAAGCAACCTAA+TGG 0.495668 6_4:+66926103 Msa0993780:intron
CAACCTAATGGCAATGATTG+TGG 0.497368 6_4:+66926115 Msa0993780:CDS
TCATCATCAGTTAATGGCGT+TGG 0.497730 6_4:-66926314 None:intergenic
CAAGAAAACAAGTTGCCCAT+TGG 0.504204 6_4:-66926355 None:intergenic
GTTAATAATTATTTGTCCCA+AGG 0.509187 6_4:+66925060 Msa0993780:five_prime_UTR
ACAACTAAACTATTGTCCTT+GGG 0.519949 6_4:-66925076 None:intergenic
CTGATACTCCTTTCTCGTAC+AGG 0.525727 6_4:-66923887 None:intergenic
AGCAAGTATCAACTCAGATA+AGG 0.542459 6_4:+66923941 Msa0993780:five_prime_UTR
TGCTCAGGGCAATAGAAAGA+AGG 0.550095 6_4:+66925268 Msa0993780:CDS
GAGGTTCATCAACTTGCTCA+GGG 0.558482 6_4:+66925254 Msa0993780:CDS
GAGCACTACCTGTACGAGAA+AGG 0.562293 6_4:+66923879 Msa0993780:five_prime_UTR
TATATGATTTGCGAAACCAA+TGG 0.577102 6_4:+66926339 Msa0993780:CDS
TAATATTACTAAGTCTTGGA+TGG 0.604101 6_4:+66926177 Msa0993780:CDS
GATCTTGGTGCATTGAACAA+AGG 0.604500 6_4:-66925099 None:intergenic
TCTACGTGTGCAGAAGACAC+TGG 0.621651 6_4:+66925035 Msa0993780:intron
TATTACTAAGTCTTGGATGG+AGG 0.643493 6_4:+66926180 Msa0993780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTTTTAATTACATA+GGG - chr6_4:66924711-66924730 None:intergenic 10.0%
!!! AAGTTTTTTTTTAATTACAT+AGG - chr6_4:66924712-66924731 None:intergenic 10.0%
!!! GAAAGAGAATTTTTTTTTAT+TGG + chr6_4:66924408-66924427 Msa0993780:intron 15.0%
!!! AAATTTGTTTCTTTACTTAT+TGG + chr6_4:66924501-66924520 Msa0993780:intron 15.0%
!! TATACTTACTTATATAGATA+TGG - chr6_4:66924959-66924978 None:intergenic 15.0%
!!! CTTTGATGATTTTTTTAATT+TGG + chr6_4:66925213-66925232 Msa0993780:intron 15.0%
!!! AGAAGATTGTAAATTTTTTA+TGG - chr6_4:66925552-66925571 None:intergenic 15.0%
!! TAAACTCAAATTATTTAGAA+AGG + chr6_4:66925778-66925797 Msa0993780:intron 15.0%
!! AAACTCAAATTATTTAGAAA+GGG + chr6_4:66925779-66925798 Msa0993780:intron 15.0%
!! GTATTATACAAAAGAATTAT+TGG - chr6_4:66925977-66925996 None:intergenic 15.0%
!! AACTAATCACTTTATAAAAA+TGG + chr6_4:66926069-66926088 Msa0993780:intron 15.0%
!!! GAATTTTTTGTAAATTGAGA+AGG - chr6_4:66923850-66923869 None:intergenic 20.0%
!!! TATTTTGCATATTTTCAGTT+TGG + chr6_4:66924330-66924349 Msa0993780:intron 20.0%
!! AAGATGTATCAATTTAGATT+AGG + chr6_4:66924462-66924481 Msa0993780:intron 20.0%
!!! GTTTTAGTATTATGATCTTT+TGG - chr6_4:66924875-66924894 None:intergenic 20.0%
!! TAATACTAAAACTAAAGTTC+AGG + chr6_4:66924883-66924902 Msa0993780:intron 20.0%
!! AATACTAAAACTAAAGTTCA+GGG + chr6_4:66924884-66924903 Msa0993780:intron 20.0%
!! TAATACAAGAAACAATCATA+CGG - chr6_4:66925359-66925378 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG + chr6_4:66925363-66925382 Msa0993780:intron 20.0%
!!! AGATTGTAAATTTTTTATGG+AGG - chr6_4:66925549-66925568 None:intergenic 20.0%
!!! TAAACCTATATGTTTTCAAT+TGG - chr6_4:66925612-66925631 None:intergenic 20.0%
!! AATTGAAAACATATAGGTTT+AGG + chr6_4:66925611-66925630 Msa0993780:intron 20.0%
!! ATTGAAAACATATAGGTTTA+GGG + chr6_4:66925612-66925631 Msa0993780:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG + chr6_4:66925810-66925829 Msa0993780:intron 20.0%
!! TTTTTGTAAATTGAGAAGGA+CGG - chr6_4:66923846-66923865 None:intergenic 25.0%
! ATGCAAAATAAACTTTGACA+TGG - chr6_4:66924320-66924339 None:intergenic 25.0%
! ACAATCAAAAACAAAGAGTA+AGG - chr6_4:66924543-66924562 None:intergenic 25.0%
!!! TTTAGTTTGTCTTCTTTTTC+AGG + chr6_4:66924679-66924698 Msa0993780:intron 25.0%
! ACGTAGAAGAACAATTATTA+AGG - chr6_4:66925022-66925041 None:intergenic 25.0%
! GTTAATAATTATTTGTCCCA+AGG + chr6_4:66925060-66925079 Msa0993780:five_prime_UTR 25.0%
!!! GTTATACATTTTAGTGTTGT+AGG - chr6_4:66925194-66925213 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG - chr6_4:66925335-66925354 None:intergenic 25.0%
! AATCATACGGAAAATATACA+GGG - chr6_4:66925346-66925365 None:intergenic 25.0%
! GTAGTGAAACAATATAATCT+TGG + chr6_4:66925465-66925484 Msa0993780:intron 25.0%
!! TAGCTTAATTGATTTAGTAG+TGG - chr6_4:66925684-66925703 None:intergenic 25.0%
! TAAAAAAATTCACTGAGGTA+CGG + chr6_4:66925815-66925834 Msa0993780:intron 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG - chr6_4:66925958-66925977 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG + chr6_4:66926177-66926196 Msa0993780:CDS 25.0%
! TATATTAACCTACACATTGT+AGG + chr6_4:66926280-66926299 Msa0993780:intron 25.0%
! TATAAGTCATCATCAGTTAA+TGG - chr6_4:66926323-66926342 None:intergenic 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG + chr6_4:66926367-66926386 Msa0993780:CDS 25.0%
TTGCTTGATGTGAGAATATA+AGG - chr6_4:66923926-66923945 None:intergenic 30.0%
!! TGCTTGCTATGTTAAAATGT+GGG + chr6_4:66924257-66924276 Msa0993780:intron 30.0%
GAAACAAATTTCTACCACAA+TGG - chr6_4:66924492-66924511 None:intergenic 30.0%
ATAAACAATATCAGCAGTCA+AGG - chr6_4:66924588-66924607 None:intergenic 30.0%
AAAGAAGACAAACTAAACGT+CGG - chr6_4:66924676-66924695 None:intergenic 30.0%
!! ACAAATAGATCGAAAGTGTT+TGG + chr6_4:66924818-66924837 Msa0993780:intron 30.0%
!! CAAATAGATCGAAAGTGTTT+GGG + chr6_4:66924819-66924838 Msa0993780:intron 30.0%
ACAACTAAACTATTGTCCTT+GGG - chr6_4:66925079-66925098 None:intergenic 30.0%
TCATTGCTTCATTAAGATCT+TGG - chr6_4:66925117-66925136 None:intergenic 30.0%
! GTTAACATATAGAGCTTTAG+AGG + chr6_4:66925235-66925254 Msa0993780:intron 30.0%
GCAATAGAAAGAAGGTTAAA+TGG + chr6_4:66925276-66925295 Msa0993780:CDS 30.0%
ACAAGAACCATACTTACTTT+GGG - chr6_4:66925308-66925327 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG - chr6_4:66925309-66925328 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG - chr6_4:66925347-66925366 None:intergenic 30.0%
GGAGGATAAACAAGAATAAA+AGG - chr6_4:66925531-66925550 None:intergenic 30.0%
GTAGCCAATTGAAAACATAT+AGG + chr6_4:66925605-66925624 Msa0993780:intron 30.0%
ATTATTTAGAAAGGGTAGTC+TGG + chr6_4:66925787-66925806 Msa0993780:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG + chr6_4:66925842-66925861 Msa0993780:intron 30.0%
ACAACAGTATCTATTCATGA+AGG - chr6_4:66925999-66926018 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG + chr6_4:66926173-66926192 Msa0993780:CDS 30.0%
! TATATGATTTGCGAAACCAA+TGG + chr6_4:66926339-66926358 Msa0993780:CDS 30.0%
! ATATGATTTGCGAAACCAAT+GGG + chr6_4:66926340-66926359 Msa0993780:CDS 30.0%
AGCAAGTATCAACTCAGATA+AGG + chr6_4:66923941-66923960 Msa0993780:five_prime_UTR 35.0%
! TAGTCGATTTCCTTGAACAT+GGG - chr6_4:66924015-66924034 None:intergenic 35.0%
AGCAATACTTGAAACACACA+AGG - chr6_4:66924038-66924057 None:intergenic 35.0%
ATAACTGTTCTGTGCTTTCA+CGG + chr6_4:66924195-66924214 Msa0993780:intron 35.0%
!! GTGCTTGCTATGTTAAAATG+TGG + chr6_4:66924256-66924275 Msa0993780:intron 35.0%
GATTGTCACATTGTGAATCT+CGG + chr6_4:66924557-66924576 Msa0993780:intron 35.0%
!! TTTTGGTTGATGCTGATGTT+AGG + chr6_4:66924639-66924658 Msa0993780:intron 35.0%
! TTTGGTTGATGCTGATGTTA+GGG + chr6_4:66924640-66924659 Msa0993780:intron 35.0%
AAGAAGACAAACTAAACGTC+GGG - chr6_4:66924675-66924694 None:intergenic 35.0%
TATTTGTCGAGATACAGAGA+CGG - chr6_4:66924805-66924824 None:intergenic 35.0%
!!! ATCGAAAGTGTTTGGGATTT+TGG + chr6_4:66924826-66924845 Msa0993780:intron 35.0%
GACTAAAATCCAAGCATACT+GGG - chr6_4:66924853-66924872 None:intergenic 35.0%
GACAACTAAACTATTGTCCT+TGG - chr6_4:66925080-66925099 None:intergenic 35.0%
CCATGTGAATGTACAGTAAA+AGG - chr6_4:66925446-66925465 None:intergenic 35.0%
! CCTTTTACTGTACATTCACA+TGG + chr6_4:66925443-66925462 Msa0993780:intron 35.0%
AAAGCAAATTGTTGTCACCA+AGG + chr6_4:66925636-66925655 Msa0993780:intron 35.0%
AAGCAAATTGTTGTCACCAA+GGG + chr6_4:66925637-66925656 Msa0993780:intron 35.0%
GATCTAACTCTGTTCTGTAT+GGG - chr6_4:66925877-66925896 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG - chr6_4:66925878-66925897 None:intergenic 35.0%
TACAGAACAGAGTTAGATCT+AGG + chr6_4:66925878-66925897 Msa0993780:intron 35.0%
! AGATCTAGGATTCCATTACA+TGG + chr6_4:66925892-66925911 Msa0993780:intron 35.0%
TGCTTCTGCTTATAAAAAGC+TGG + chr6_4:66925922-66925941 Msa0993780:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG - chr6_4:66926095-66926114 None:intergenic 35.0%
TATCCACAATCATTGCCATT+AGG - chr6_4:66926121-66926140 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG + chr6_4:66926180-66926199 Msa0993780:CDS 35.0%
ATTGAAGACCTACAATGTGT+AGG - chr6_4:66926291-66926310 None:intergenic 35.0%
TCTCACTTCACCATGTATGT+TGG + chr6_4:66923981-66924000 Msa0993780:intron 40.0%
! GTAGTCGATTTCCTTGAACA+TGG - chr6_4:66924016-66924035 None:intergenic 40.0%
TTTGTAAGCACAACAGTGGT+GGG - chr6_4:66924135-66924154 None:intergenic 40.0%
ACACTTTGTAAGCACAACAG+TGG - chr6_4:66924139-66924158 None:intergenic 40.0%
TAACTGTTCTGTGCTTTCAC+GGG + chr6_4:66924196-66924215 Msa0993780:intron 40.0%
TTAGATTAGGAGCTCCATTG+TGG + chr6_4:66924475-66924494 Msa0993780:intron 40.0%
! TCCAGCTCAATTCTCTCTTT+TGG + chr6_4:66924622-66924641 Msa0993780:intron 40.0%
ACCAAAAGAGAGAATTGAGC+TGG - chr6_4:66924626-66924645 None:intergenic 40.0%
! TTGGTTGATGCTGATGTTAG+GGG + chr6_4:66924641-66924660 Msa0993780:intron 40.0%
AAAACTGTCACTGAGTATGG+TGG - chr6_4:66924737-66924756 None:intergenic 40.0%
GCAAAAACTGTCACTGAGTA+TGG - chr6_4:66924740-66924759 None:intergenic 40.0%
GGACTAAAATCCAAGCATAC+TGG - chr6_4:66924854-66924873 None:intergenic 40.0%
TTCAGGGACACATACAAACT+AGG + chr6_4:66924900-66924919 Msa0993780:intron 40.0%
! GATCTTGGTGCATTGAACAA+AGG - chr6_4:66925102-66925121 None:intergenic 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG + chr6_4:66925298-66925317 Msa0993780:intron 40.0%
AAGCATGTGAGTCCATGTAA+TGG - chr6_4:66925907-66925926 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG + chr6_4:66926103-66926122 Msa0993780:intron 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG - chr6_4:66926109-66926128 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG + chr6_4:66926115-66926134 Msa0993780:CDS 40.0%
TCATCATCAGTTAATGGCGT+TGG - chr6_4:66926317-66926336 None:intergenic 40.0%
CAAGAAAACAAGTTGCCCAT+TGG - chr6_4:66926358-66926377 None:intergenic 40.0%
CTGATACTCCTTTCTCGTAC+AGG - chr6_4:66923890-66923909 None:intergenic 45.0%
GGTAACTGCTCCAACATACA+TGG - chr6_4:66923994-66924013 None:intergenic 45.0%
! AGTGGTGGGACTCAAAGAAA+TGG - chr6_4:66924121-66924140 None:intergenic 45.0%
CTTTGTAAGCACAACAGTGG+TGG - chr6_4:66924136-66924155 None:intergenic 45.0%
AGATACGTATCCACCAGTCA+AGG + chr6_4:66924371-66924390 Msa0993780:intron 45.0%
! GATTTTGGACCCAGTATGCT+TGG + chr6_4:66924841-66924860 Msa0993780:intron 45.0%
! AGAGGTTCATCAACTTGCTC+AGG + chr6_4:66925253-66925272 Msa0993780:CDS 45.0%
! GAGGTTCATCAACTTGCTCA+GGG + chr6_4:66925254-66925273 Msa0993780:CDS 45.0%
TGCTCAGGGCAATAGAAAGA+AGG + chr6_4:66925268-66925287 Msa0993780:CDS 45.0%
CTAAGAAACGAACACACCCT+TGG - chr6_4:66925656-66925675 None:intergenic 45.0%
!! ATAAGTAATTAATATTAATA+AGG - chr6_4:66926263-66926282 None:intergenic 5.0%
GAGCACTACCTGTACGAGAA+AGG + chr6_4:66923879-66923898 Msa0993780:five_prime_UTR 50.0%
GGAGCAGTTACCCATGTTCA+AGG + chr6_4:66924002-66924021 Msa0993780:intron 50.0%
AAGTCGTGAACCTTGACTGG+TGG - chr6_4:66924384-66924403 None:intergenic 50.0%
TCGAAGTCGTGAACCTTGAC+TGG - chr6_4:66924387-66924406 None:intergenic 50.0%
TTGTCGAGATACAGAGACGG+AGG - chr6_4:66924802-66924821 None:intergenic 50.0%
TCTACGTGTGCAGAAGACAC+TGG + chr6_4:66925035-66925054 Msa0993780:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 66923808 66926394 66923808 ID=Msa0993780;Name=Msa0993780
chr6_4 mRNA 66923808 66926394 66923808 ID=Msa0993780-mRNA-1;Parent=Msa0993780;Name=Msa0993780-mRNA-1;_AED=0.18;_eAED=0.18;_QI=257|0.66|0.57|0.71|0.5|0.42|7|0|98
chr6_4 exon 66923808 66923962 66923808 ID=Msa0993780-mRNA-1:exon:12468;Parent=Msa0993780-mRNA-1
chr6_4 exon 66924701 66924713 66924701 ID=Msa0993780-mRNA-1:exon:12469;Parent=Msa0993780-mRNA-1
chr6_4 exon 66924942 66924969 66924942 ID=Msa0993780-mRNA-1:exon:12470;Parent=Msa0993780-mRNA-1
chr6_4 exon 66925048 66925152 66925048 ID=Msa0993780-mRNA-1:exon:12471;Parent=Msa0993780-mRNA-1
chr6_4 exon 66925247 66925310 66925247 ID=Msa0993780-mRNA-1:exon:12472;Parent=Msa0993780-mRNA-1
chr6_4 exon 66926106 66926201 66926106 ID=Msa0993780-mRNA-1:exon:12473;Parent=Msa0993780-mRNA-1
chr6_4 exon 66926302 66926394 66926302 ID=Msa0993780-mRNA-1:exon:12474;Parent=Msa0993780-mRNA-1
chr6_4 five_prime_UTR 66923808 66923962 66923808 ID=Msa0993780-mRNA-1:five_prime_utr;Parent=Msa0993780-mRNA-1
chr6_4 five_prime_UTR 66924701 66924713 66924701 ID=Msa0993780-mRNA-1:five_prime_utr;Parent=Msa0993780-mRNA-1
chr6_4 five_prime_UTR 66924942 66924969 66924942 ID=Msa0993780-mRNA-1:five_prime_utr;Parent=Msa0993780-mRNA-1
chr6_4 five_prime_UTR 66925048 66925108 66925048 ID=Msa0993780-mRNA-1:five_prime_utr;Parent=Msa0993780-mRNA-1
chr6_4 CDS 66925109 66925152 66925109 ID=Msa0993780-mRNA-1:cds;Parent=Msa0993780-mRNA-1
chr6_4 CDS 66925247 66925310 66925247 ID=Msa0993780-mRNA-1:cds;Parent=Msa0993780-mRNA-1
chr6_4 CDS 66926106 66926201 66926106 ID=Msa0993780-mRNA-1:cds;Parent=Msa0993780-mRNA-1
chr6_4 CDS 66926302 66926394 66926302 ID=Msa0993780-mRNA-1:cds;Parent=Msa0993780-mRNA-1
Gene Sequence

>Msa0993780

ATGCACCAAGATCTTAATGAAGCAATGATTAAGATTGTTTCAAAAGCTTTAGAGGTTCATCAACTTGCTCAGGGCAATAGAAAGAAGGTTAAATGGTTTCGTCCCAAACCAAGAAAGCAACCTAATGGCAATGATTGTGGATACTATGTGATGAAGAATATGCTAGATATCGTCTCTGCTAATATTACTAAGTCTTGGATGGAGGTCTTCAATGATCCAACGCCATTAACTGATGATGACTTATATGATTTGCGAAACCAATGGGCAACTTGTTTTCTTGACTTGTATAAGGCTTAG

Protein sequence

>Msa0993780

MHQDLNEAMIKIVSKALEVHQLAQGNRKKVKWFRPKPRKQPNGNDCGYYVMKNMLDIVSANITKSWMEVFNDPTPLTDDDLYDLRNQWATCFLDLYKA*