AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0929070


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0929070 MtrunA17_Chr1g0174011 92.708 96 7 0 1 96 1 96 5.33e-66 193
Msa0929070 MtrunA17_Chr5g0434431 92.708 96 7 0 1 96 79 174 5.57e-65 193
Msa0929070 MtrunA17_Chr6g0469071 91.837 98 8 0 1 98 521 618 5.45e-62 198
Msa0929070 MtrunA17_Chr1g0153911 92.222 90 7 0 9 98 1 90 2.31e-60 179
Msa0929070 MtrunA17_Chr4g0033251 79.592 49 9 1 51 98 1 49 1.10e-22 82.8
Msa0929070 MtrunA17_Chr2g0321271 68.750 48 15 0 51 98 1 48 1.24e-17 70.1
Msa0929070 MtrunA17_Chr2g0325841 36.170 94 59 1 6 98 1105 1198 1.38e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCATTAGGTTGCTTTCT+TGG 0.248489 6_2:+76284476 None:intergenic
GCAATAGAAAGAAGGTTAAA+TGG 0.271188 6_2:-76285306 Msa0929070:CDS
TTGCTTGATGTGAGAATATA+AGG 0.310732 6_2:+76286659 None:intergenic
GTTAACATATAGAGCTTTAG+AGG 0.328169 6_2:-76285347 Msa0929070:intron
TCATTGCTTCATTAAGATCT+TGG 0.330442 6_2:+76285468 None:intergenic
TATAAGTCATCATCAGTTAA+TGG 0.351177 6_2:+76284262 None:intergenic
TCTTGACTTGTATAAGGCTT+AGG 0.381448 6_2:-76284209 None:intergenic
AGAGGTTCATCAACTTGCTC+AGG 0.406730 6_2:-76285329 Msa0929070:CDS
GACAACTAAACTATTGTCCT+TGG 0.409668 6_2:+76285505 None:intergenic
TATCCACAATCATTGCCATT+AGG 0.411955 6_2:+76284464 None:intergenic
TATACTTACTTATATAGATA+TGG 0.415179 6_2:+76285626 None:intergenic
CTGCTAATATTACTAAGTCT+TGG 0.472781 6_2:-76284409 Msa0929070:CDS
ATATGATTTGCGAAACCAAT+GGG 0.486055 6_2:-76284242 Msa0929070:CDS
TAGCCAAGAAAGCAACCTAA+TGG 0.495668 6_2:-76284479 Msa0929070:CDS
CAACCTAATGGCAATGATTG+TGG 0.497368 6_2:-76284467 Msa0929070:CDS
TCATCATCAGTTAATGGCGT+TGG 0.497730 6_2:+76284268 None:intergenic
CAAGAAAACAAGTTGCCCAT+TGG 0.504204 6_2:+76284227 None:intergenic
GTTAATAATTATTTGTCCCA+AGG 0.509187 6_2:-76285522 Msa0929070:five_prime_UTR
ACAACTAAACTATTGTCCTT+GGG 0.519949 6_2:+76285506 None:intergenic
CTGATACTCCTTTCTCGTAC+AGG 0.525727 6_2:+76286695 None:intergenic
AGCAAGTATCAACTCAGATA+AGG 0.542459 6_2:-76286641 Msa0929070:intron
TGCTCAGGGCAATAGAAAGA+AGG 0.550095 6_2:-76285314 Msa0929070:CDS
GAGGTTCATCAACTTGCTCA+GGG 0.558482 6_2:-76285328 Msa0929070:CDS
GAGCACTACCTGTACGAGAA+AGG 0.562293 6_2:-76286703 Msa0929070:five_prime_UTR
TATATGATTTGCGAAACCAA+TGG 0.577102 6_2:-76284243 Msa0929070:CDS
TAATATTACTAAGTCTTGGA+TGG 0.604101 6_2:-76284405 Msa0929070:CDS
GATCTTGGTGCATTGAACAA+AGG 0.604500 6_2:+76285483 None:intergenic
TCTACGTGTGCAGAAGACAC+TGG 0.621651 6_2:-76285547 Msa0929070:intron
TATTACTAAGTCTTGGATGG+AGG 0.643493 6_2:-76284402 Msa0929070:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTTTTTAATTACATA+GGG + chr6_2:76285113-76285132 None:intergenic 10.0%
!!! AAGTTTTTTTTTAATTACAT+AGG + chr6_2:76285114-76285133 None:intergenic 10.0%
!!! GAAAGAGAATTTTTTTTTAT+TGG - chr6_2:76284810-76284829 Msa0929070:intron 15.0%
!!! AAATTTGTTTCTTTACTTAT+TGG - chr6_2:76284903-76284922 Msa0929070:intron 15.0%
!! TATACTTACTTATATAGATA+TGG + chr6_2:76285361-76285380 None:intergenic 15.0%
!!! CTTTGATGATTTTTTTAATT+TGG - chr6_2:76285615-76285634 Msa0929070:intron 15.0%
!!! AGAAGATTGTAAATTTTTTA+TGG + chr6_2:76285954-76285973 None:intergenic 15.0%
!! TAAACTCAAATTATTTAGAA+AGG - chr6_2:76286180-76286199 Msa0929070:intron 15.0%
!! AAACTCAAATTATTTAGAAA+GGG - chr6_2:76286181-76286200 Msa0929070:intron 15.0%
!! GTATTATACAAAAGAATTAT+TGG + chr6_2:76286379-76286398 None:intergenic 15.0%
!! AACTAATCACTTTATAAAAA+TGG - chr6_2:76286471-76286490 Msa0929070:intron 15.0%
!!! GAATTTTTTGTAAATTGAGA+AGG + chr6_2:76284252-76284271 None:intergenic 20.0%
!!! TATTTTGCATATTTTCAGTT+TGG - chr6_2:76284732-76284751 Msa0929070:intron 20.0%
!! AAGATGTATCAATTTAGATT+AGG - chr6_2:76284864-76284883 Msa0929070:intron 20.0%
!!! GTTTTAGTATTATGATCTTT+TGG + chr6_2:76285277-76285296 None:intergenic 20.0%
!! TAATACTAAAACTAAAGTTC+AGG - chr6_2:76285285-76285304 Msa0929070:intron 20.0%
!! AATACTAAAACTAAAGTTCA+GGG - chr6_2:76285286-76285305 Msa0929070:intron 20.0%
!! TAATACAAGAAACAATCATA+CGG + chr6_2:76285761-76285780 None:intergenic 20.0%
!!! ATTGTTTCTTGTATTAAAAG+TGG - chr6_2:76285765-76285784 Msa0929070:intron 20.0%
!!! AGATTGTAAATTTTTTATGG+AGG + chr6_2:76285951-76285970 None:intergenic 20.0%
!!! TAAACCTATATGTTTTCAAT+TGG + chr6_2:76286014-76286033 None:intergenic 20.0%
!! AATTGAAAACATATAGGTTT+AGG - chr6_2:76286013-76286032 Msa0929070:intron 20.0%
!! ATTGAAAACATATAGGTTTA+GGG - chr6_2:76286014-76286033 Msa0929070:intron 20.0%
!! TAAAGTAAAAAAATTCACTG+AGG - chr6_2:76286212-76286231 Msa0929070:intron 20.0%
!! TTTTTGTAAATTGAGAAGGA+CGG + chr6_2:76284248-76284267 None:intergenic 25.0%
! ATGCAAAATAAACTTTGACA+TGG + chr6_2:76284722-76284741 None:intergenic 25.0%
! ACAATCAAAAACAAAGAGTA+AGG + chr6_2:76284945-76284964 None:intergenic 25.0%
!!! TTTAGTTTGTCTTCTTTTTC+AGG - chr6_2:76285081-76285100 Msa0929070:intron 25.0%
! ACGTAGAAGAACAATTATTA+AGG + chr6_2:76285424-76285443 None:intergenic 25.0%
! GTTAATAATTATTTGTCCCA+AGG - chr6_2:76285462-76285481 Msa0929070:CDS 25.0%
!!! GTTATACATTTTAGTGTTGT+AGG + chr6_2:76285596-76285615 None:intergenic 25.0%
! AAATATACAGGGAACAATTA+AGG + chr6_2:76285737-76285756 None:intergenic 25.0%
! AATCATACGGAAAATATACA+GGG + chr6_2:76285748-76285767 None:intergenic 25.0%
! GTAGTGAAACAATATAATCT+TGG - chr6_2:76285867-76285886 Msa0929070:intron 25.0%
!! TAGCTTAATTGATTTAGTAG+TGG + chr6_2:76286086-76286105 None:intergenic 25.0%
! TAAAAAAATTCACTGAGGTA+CGG - chr6_2:76286217-76286236 Msa0929070:intron 25.0%
! TTGGTTAAGTAGTGTAAAAA+AGG + chr6_2:76286360-76286379 None:intergenic 25.0%
! TAATATTACTAAGTCTTGGA+TGG - chr6_2:76286579-76286598 Msa0929070:intron 25.0%
! TATATTAACCTACACATTGT+AGG - chr6_2:76286682-76286701 Msa0929070:five_prime_UTR 25.0%
! TATAAGTCATCATCAGTTAA+TGG + chr6_2:76286725-76286744 None:intergenic 25.0%
!!! TTGTTTTCTTGACTTGTATA+AGG - chr6_2:76286769-76286788 Msa0929070:five_prime_UTR 25.0%
TTGCTTGATGTGAGAATATA+AGG + chr6_2:76284328-76284347 None:intergenic 30.0%
!! TGCTTGCTATGTTAAAATGT+GGG - chr6_2:76284659-76284678 Msa0929070:intron 30.0%
GAAACAAATTTCTACCACAA+TGG + chr6_2:76284894-76284913 None:intergenic 30.0%
ATAAACAATATCAGCAGTCA+AGG + chr6_2:76284990-76285009 None:intergenic 30.0%
AAAGAAGACAAACTAAACGT+CGG + chr6_2:76285078-76285097 None:intergenic 30.0%
!! ACAAATAGATCGAAAGTGTT+TGG - chr6_2:76285220-76285239 Msa0929070:intron 30.0%
!! CAAATAGATCGAAAGTGTTT+GGG - chr6_2:76285221-76285240 Msa0929070:intron 30.0%
ACAACTAAACTATTGTCCTT+GGG + chr6_2:76285481-76285500 None:intergenic 30.0%
TCATTGCTTCATTAAGATCT+TGG + chr6_2:76285519-76285538 None:intergenic 30.0%
! GTTAACATATAGAGCTTTAG+AGG - chr6_2:76285637-76285656 Msa0929070:five_prime_UTR 30.0%
GCAATAGAAAGAAGGTTAAA+TGG - chr6_2:76285678-76285697 Msa0929070:intron 30.0%
ACAAGAACCATACTTACTTT+GGG + chr6_2:76285710-76285729 None:intergenic 30.0%
AACAAGAACCATACTTACTT+TGG + chr6_2:76285711-76285730 None:intergenic 30.0%
CAATCATACGGAAAATATAC+AGG + chr6_2:76285749-76285768 None:intergenic 30.0%
GGAGGATAAACAAGAATAAA+AGG + chr6_2:76285933-76285952 None:intergenic 30.0%
GTAGCCAATTGAAAACATAT+AGG - chr6_2:76286007-76286026 Msa0929070:intron 30.0%
ATTATTTAGAAAGGGTAGTC+TGG - chr6_2:76286189-76286208 Msa0929070:intron 30.0%
! TAGACTTAGCTTGATTACAT+TGG - chr6_2:76286244-76286263 Msa0929070:intron 30.0%
ACAACAGTATCTATTCATGA+AGG + chr6_2:76286401-76286420 None:intergenic 30.0%
CTGCTAATATTACTAAGTCT+TGG - chr6_2:76286575-76286594 Msa0929070:intron 30.0%
! TATATGATTTGCGAAACCAA+TGG - chr6_2:76286741-76286760 Msa0929070:five_prime_UTR 30.0%
! ATATGATTTGCGAAACCAAT+GGG - chr6_2:76286742-76286761 Msa0929070:five_prime_UTR 30.0%
AGCAAGTATCAACTCAGATA+AGG - chr6_2:76284343-76284362 Msa0929070:intron 35.0%
! TAGTCGATTTCCTTGAACAT+GGG + chr6_2:76284417-76284436 None:intergenic 35.0%
AGCAATACTTGAAACACACA+AGG + chr6_2:76284440-76284459 None:intergenic 35.0%
ATAACTGTTCTGTGCTTTCA+CGG - chr6_2:76284597-76284616 Msa0929070:intron 35.0%
!! GTGCTTGCTATGTTAAAATG+TGG - chr6_2:76284658-76284677 Msa0929070:intron 35.0%
GATTGTCACATTGTGAATCT+CGG - chr6_2:76284959-76284978 Msa0929070:intron 35.0%
!! TTTTGGTTGATGCTGATGTT+AGG - chr6_2:76285041-76285060 Msa0929070:intron 35.0%
! TTTGGTTGATGCTGATGTTA+GGG - chr6_2:76285042-76285061 Msa0929070:intron 35.0%
AAGAAGACAAACTAAACGTC+GGG + chr6_2:76285077-76285096 None:intergenic 35.0%
TATTTGTCGAGATACAGAGA+CGG + chr6_2:76285207-76285226 None:intergenic 35.0%
!!! ATCGAAAGTGTTTGGGATTT+TGG - chr6_2:76285228-76285247 Msa0929070:intron 35.0%
GACTAAAATCCAAGCATACT+GGG + chr6_2:76285255-76285274 None:intergenic 35.0%
GACAACTAAACTATTGTCCT+TGG + chr6_2:76285482-76285501 None:intergenic 35.0%
CCATGTGAATGTACAGTAAA+AGG + chr6_2:76285848-76285867 None:intergenic 35.0%
! CCTTTTACTGTACATTCACA+TGG - chr6_2:76285845-76285864 Msa0929070:intron 35.0%
AAAGCAAATTGTTGTCACCA+AGG - chr6_2:76286038-76286057 Msa0929070:intron 35.0%
AAGCAAATTGTTGTCACCAA+GGG - chr6_2:76286039-76286058 Msa0929070:intron 35.0%
GATCTAACTCTGTTCTGTAT+GGG + chr6_2:76286279-76286298 None:intergenic 35.0%
AGATCTAACTCTGTTCTGTA+TGG + chr6_2:76286280-76286299 None:intergenic 35.0%
TACAGAACAGAGTTAGATCT+AGG - chr6_2:76286280-76286299 Msa0929070:intron 35.0%
! AGATCTAGGATTCCATTACA+TGG - chr6_2:76286294-76286313 Msa0929070:intron 35.0%
TGCTTCTGCTTATAAAAAGC+TGG - chr6_2:76286324-76286343 Msa0929070:intron 35.0%
CTTTCTTGGCTACAAATGAA+TGG + chr6_2:76286497-76286516 None:intergenic 35.0%
TATCCACAATCATTGCCATT+AGG + chr6_2:76286523-76286542 None:intergenic 35.0%
TATTACTAAGTCTTGGATGG+AGG - chr6_2:76286582-76286601 Msa0929070:intron 35.0%
ATTGAAGACCTACAATGTGT+AGG + chr6_2:76286693-76286712 None:intergenic 35.0%
TCTCACTTCACCATGTATGT+TGG - chr6_2:76284383-76284402 Msa0929070:intron 40.0%
! GTAGTCGATTTCCTTGAACA+TGG + chr6_2:76284418-76284437 None:intergenic 40.0%
TTTGTAAGCACAACAGTGGT+GGG + chr6_2:76284537-76284556 None:intergenic 40.0%
ACACTTTGTAAGCACAACAG+TGG + chr6_2:76284541-76284560 None:intergenic 40.0%
TAACTGTTCTGTGCTTTCAC+GGG - chr6_2:76284598-76284617 Msa0929070:intron 40.0%
TTAGATTAGGAGCTCCATTG+TGG - chr6_2:76284877-76284896 Msa0929070:intron 40.0%
! TCCAGCTCAATTCTCTCTTT+TGG - chr6_2:76285024-76285043 Msa0929070:intron 40.0%
ACCAAAAGAGAGAATTGAGC+TGG + chr6_2:76285028-76285047 None:intergenic 40.0%
! TTGGTTGATGCTGATGTTAG+GGG - chr6_2:76285043-76285062 Msa0929070:intron 40.0%
AAAACTGTCACTGAGTATGG+TGG + chr6_2:76285139-76285158 None:intergenic 40.0%
GCAAAAACTGTCACTGAGTA+TGG + chr6_2:76285142-76285161 None:intergenic 40.0%
GGACTAAAATCCAAGCATAC+TGG + chr6_2:76285256-76285275 None:intergenic 40.0%
TTCAGGGACACATACAAACT+AGG - chr6_2:76285302-76285321 Msa0929070:CDS 40.0%
! GATCTTGGTGCATTGAACAA+AGG + chr6_2:76285504-76285523 None:intergenic 40.0%
GTTTCGTCCCAAAGTAAGTA+TGG - chr6_2:76285700-76285719 Msa0929070:intron 40.0%
AAGCATGTGAGTCCATGTAA+TGG + chr6_2:76286309-76286328 None:intergenic 40.0%
TAGCCAAGAAAGCAACCTAA+TGG - chr6_2:76286505-76286524 Msa0929070:intron 40.0%
! TTGCCATTAGGTTGCTTTCT+TGG + chr6_2:76286511-76286530 None:intergenic 40.0%
CAACCTAATGGCAATGATTG+TGG - chr6_2:76286517-76286536 Msa0929070:intron 40.0%
TCATCATCAGTTAATGGCGT+TGG + chr6_2:76286719-76286738 None:intergenic 40.0%
CAAGAAAACAAGTTGCCCAT+TGG + chr6_2:76286760-76286779 None:intergenic 40.0%
CTGATACTCCTTTCTCGTAC+AGG + chr6_2:76284292-76284311 None:intergenic 45.0%
GGTAACTGCTCCAACATACA+TGG + chr6_2:76284396-76284415 None:intergenic 45.0%
! AGTGGTGGGACTCAAAGAAA+TGG + chr6_2:76284523-76284542 None:intergenic 45.0%
CTTTGTAAGCACAACAGTGG+TGG + chr6_2:76284538-76284557 None:intergenic 45.0%
AGATACGTATCCACCAGTCA+AGG - chr6_2:76284773-76284792 Msa0929070:intron 45.0%
! GATTTTGGACCCAGTATGCT+TGG - chr6_2:76285243-76285262 Msa0929070:intron 45.0%
! AGAGGTTCATCAACTTGCTC+AGG - chr6_2:76285655-76285674 Msa0929070:intron 45.0%
! GAGGTTCATCAACTTGCTCA+GGG - chr6_2:76285656-76285675 Msa0929070:intron 45.0%
TGCTCAGGGCAATAGAAAGA+AGG - chr6_2:76285670-76285689 Msa0929070:intron 45.0%
CTAAGAAACGAACACACCCT+TGG + chr6_2:76286058-76286077 None:intergenic 45.0%
!! ATAAGTAATTAATATTAATA+AGG + chr6_2:76286665-76286684 None:intergenic 5.0%
GAGCACTACCTGTACGAGAA+AGG - chr6_2:76284281-76284300 Msa0929070:CDS 50.0%
GGAGCAGTTACCCATGTTCA+AGG - chr6_2:76284404-76284423 Msa0929070:CDS 50.0%
AAGTCGTGAACCTTGACTGG+TGG + chr6_2:76284786-76284805 None:intergenic 50.0%
TCGAAGTCGTGAACCTTGAC+TGG + chr6_2:76284789-76284808 None:intergenic 50.0%
TTGTCGAGATACAGAGACGG+AGG + chr6_2:76285204-76285223 None:intergenic 50.0%
TCTACGTGTGCAGAAGACAC+TGG - chr6_2:76285437-76285456 Msa0929070:intron 50.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 76284210 76286796 76284210 ID=Msa0929070;Name=Msa0929070
chr6_2 mRNA 76284210 76286796 76284210 ID=Msa0929070-mRNA-1;Parent=Msa0929070;Name=Msa0929070-mRNA-1;_AED=0.18;_eAED=0.18;_QI=257|0.66|0.57|0.71|0.5|0.42|7|0|98
chr6_2 exon 76284210 76284302 76284210 ID=Msa0929070-mRNA-1:exon:12856;Parent=Msa0929070-mRNA-1
chr6_2 exon 76284403 76284498 76284403 ID=Msa0929070-mRNA-1:exon:12855;Parent=Msa0929070-mRNA-1
chr6_2 exon 76285294 76285357 76285294 ID=Msa0929070-mRNA-1:exon:12854;Parent=Msa0929070-mRNA-1
chr6_2 exon 76285452 76285556 76285452 ID=Msa0929070-mRNA-1:exon:12853;Parent=Msa0929070-mRNA-1
chr6_2 exon 76285635 76285662 76285635 ID=Msa0929070-mRNA-1:exon:12852;Parent=Msa0929070-mRNA-1
chr6_2 exon 76285891 76285903 76285891 ID=Msa0929070-mRNA-1:exon:12851;Parent=Msa0929070-mRNA-1
chr6_2 exon 76286642 76286796 76286642 ID=Msa0929070-mRNA-1:exon:12850;Parent=Msa0929070-mRNA-1
chr6_2 five_prime_UTR 76286642 76286796 76286642 ID=Msa0929070-mRNA-1:five_prime_utr;Parent=Msa0929070-mRNA-1
chr6_2 five_prime_UTR 76285891 76285903 76285891 ID=Msa0929070-mRNA-1:five_prime_utr;Parent=Msa0929070-mRNA-1
chr6_2 five_prime_UTR 76285635 76285662 76285635 ID=Msa0929070-mRNA-1:five_prime_utr;Parent=Msa0929070-mRNA-1
chr6_2 five_prime_UTR 76285496 76285556 76285496 ID=Msa0929070-mRNA-1:five_prime_utr;Parent=Msa0929070-mRNA-1
chr6_2 CDS 76285452 76285495 76285452 ID=Msa0929070-mRNA-1:cds;Parent=Msa0929070-mRNA-1
chr6_2 CDS 76285294 76285357 76285294 ID=Msa0929070-mRNA-1:cds;Parent=Msa0929070-mRNA-1
chr6_2 CDS 76284403 76284498 76284403 ID=Msa0929070-mRNA-1:cds;Parent=Msa0929070-mRNA-1
chr6_2 CDS 76284210 76284302 76284210 ID=Msa0929070-mRNA-1:cds;Parent=Msa0929070-mRNA-1
Gene Sequence

>Msa0929070

ATGCACCAAGATCTTAATGAAGCAATGATTAAGATTGTTTCAAAAGCTTTAGAGGTTCATCAACTTGCTCAGGGCAATAGAAAGAAGGTTAAATGGTTTCGTCCCAAACCAAGAAAGCAACCTAATGGCAATGATTGTGGATACTATGTGATGAAGAATATGCTAGATATCGTCTCTGCTAATATTACTAAGTCTTGGATGGAGGTCTTCAATGATCCAACGCCATTAACTGATGATGACTTATATGATTTGCGAAACCAATGGGCAACTTGTTTTCTTGACTTGTATAAGGCTTAG

Protein sequence

>Msa0929070

MHQDLNEAMIKIVSKALEVHQLAQGNRKKVKWFRPKPRKQPNGNDCGYYVMKNMLDIVSANITKSWMEVFNDPTPLTDDDLYDLRNQWATCFLDLYKA*