AlfalfaGEDB | Msa1009130

M. sativa cultivar zhongmu-4 / Msa1009130

KEGG: -
GO: -
PFAM: PF03031.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1009130	XP_039683359.1	95.370	324	15	0	1	324	1	324	0.0	618

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1009130	A0A072TW70	95.370	324	15	0	1	324	1	324	0.0	618

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0056030	Msa1009130	0.809254	2.072445e-50	-8.615850e-47
Msa0057140	Msa1009130	0.830517	2.808625e-55	-8.615850e-47
Msa0467780	Msa1009130	0.811315	7.443126e-51	-8.615850e-47
Msa0600600	Msa1009130	0.807677	4.497561e-50	-8.615850e-47
Msa0641140	Msa1009130	0.826791	2.234248e-54	-8.615850e-47
Msa0694840	Msa1009130	0.802711	4.928364e-49	-8.615850e-47
Msa0827280	Msa1009130	0.810698	1.012620e-50	-8.615850e-47
Msa1009130	Msa1040800	0.814600	1.418472e-51	-8.615850e-47
Msa1009130	Msa1050420	0.811663	6.254709e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1009130	MtrunA17_Chr7g0219551	86.111	324	29	2	1	324	1	308	0.0	537
Msa1009130	MtrunA17_Chr7g0219541	58.400	250	83	4	79	324	39	271	3.41e-100	296
Msa1009130	MtrunA17_Chr7g0219531	54.122	279	104	6	51	323	4	264	2.75e-99	293
Msa1009130	MtrunA17_Chr7g0219521	50.661	227	84	5	54	272	11	217	2.82e-68	213
Msa1009130	MtrunA17_Chr2g0308461	43.154	241	109	3	88	321	281	500	2.99e-63	209
Msa1009130	MtrunA17_Chr7g0265691	34.034	238	131	6	88	320	42	258	5.99e-36	130
Msa1009130	MtrunA17_Chr7g0265701	32.946	258	144	6	64	313	8	244	6.11e-35	127
Msa1009130	MtrunA17_Chr7g0265581	30.102	196	118	3	123	318	33	209	1.66e-27	107
Msa1009130	MtrunA17_Chr7g0219511	61.818	55	18	1	273	324	16	70	8.92e-17	73.9
Msa1009130	MtrunA17_Chr7g0255231	29.605	152	84	5	106	256	97	226	3.68e-11	62.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1009130	AT3G29760.2	48.052	231	108	6	101	321	318	546	5.35e-68	222
Msa1009130	AT3G29760.5	47.964	221	97	5	101	321	318	520	6.48e-68	221
Msa1009130	AT3G29760.3	47.964	221	97	5	101	321	318	520	6.48e-68	221
Msa1009130	AT4G26190.2	41.048	229	117	3	89	317	843	1053	1.22e-52	186
Msa1009130	AT4G26190.5	41.048	229	117	3	89	317	843	1053	1.22e-52	186
Msa1009130	AT4G26190.3	41.048	229	117	3	89	317	843	1053	1.22e-52	186
Msa1009130	AT4G26190.1	41.048	229	117	3	89	317	843	1053	1.22e-52	186
Msa1009130	AT4G26190.4	41.048	229	117	3	89	317	843	1053	1.22e-52	186
Msa1009130	AT3G29760.1	47.742	155	63	5	101	255	318	454	4.60e-39	144
Msa1009130	AT2G36540.1	34.434	212	104	4	109	320	72	248	2.09e-31	118

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Find 66 sgRNAs with CRISPR-Local

Find 441 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGGTGCCTTTCAAC+TGG	0.405289	7_1:-10023076	Msa1009130:three_prime_UTR
AAATTGATATTCCAGTTGAA+AGG	0.583747	7_1:+10023065	None:intergenic
ACGACAATATCTAGAAAAGT+TGG	0.503354	7_1:-10023223	Msa1009130:CDS
ACGCAAGACAGTGGTGTTTA+AGG	0.242780	7_1:-10024857	Msa1009130:CDS
AGAAATTTGATGTGGCTGTG+TGG	0.669495	7_1:-10025587	Msa1009130:CDS
AGAATGTCGTGAATTTCTCG+CGG	0.717507	7_1:+10028703	None:intergenic
AGAGAAAACTGAGTAAGCAG+AGG	0.609024	7_1:-10028562	Msa1009130:CDS
AGGATTAAGTAACCCCTTGT+AGG	0.482288	7_1:+10024741	None:intergenic
AGTCCGATCCGAATCTTCCT+TGG	0.452305	7_1:-10024813	Msa1009130:CDS
ATGTCGTGAATTTCTCGCGG+CGG	0.725491	7_1:+10028706	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCCCACAATTTAAAGAGTT+AGG	+	chr7_1:10023298-10023317	None:intergenic	30.0%
TCATACCAACATCACTAAAA+TGG	+	chr7_1:10023507-10023526	None:intergenic	30.0%
TTAGTGATGTTGGTATGAAA+CGG	-	chr7_1:10023509-10023528	Msa1009130:intron	30.0%
AAGTGTTCCAAAGTTATTCA+CGG	-	chr7_1:10023588-10023607	Msa1009130:intron	30.0%
CAAGGATCATCTCATAAATT+TGG	+	chr7_1:10023663-10023682	None:intergenic	30.0%
GCAAATTAATGCAAATCACA+AGG	+	chr7_1:10023681-10023700	None:intergenic	30.0%
ATTTCAACGATACTTGAGAA+TGG	-	chr7_1:10025155-10025174	Msa1009130:intron	30.0%
GAAAAAGTGATGTTTCCAAT+CGG	+	chr7_1:10025319-10025338	None:intergenic	30.0%
GTAATGTTGTCTTGAGATAT+TGG	-	chr7_1:10025387-10025406	Msa1009130:intron	30.0%
TCTTGAGATATTGGAAATAG+AGG	-	chr7_1:10025396-10025415	Msa1009130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	10022853	10028815	10022853	ID=Msa1009130;Name=Msa1009130
chr7_1	mRNA	10022853	10028815	10022853	ID=Msa1009130-mRNA-1;Parent=Msa1009130;Name=Msa1009130-mRNA-1;_AED=0.42;_eAED=0.42;_QI=137\|0.8\|0.83\|1\|1\|1\|6\|245\|324
chr7_1	exon	10022853	10023261	10022853	ID=Msa1009130-mRNA-1:exon:2829;Parent=Msa1009130-mRNA-1
chr7_1	exon	10024583	10024643	10024583	ID=Msa1009130-mRNA-1:exon:2828;Parent=Msa1009130-mRNA-1
chr7_1	exon	10024741	10024926	10024741	ID=Msa1009130-mRNA-1:exon:2827;Parent=Msa1009130-mRNA-1
chr7_1	exon	10025458	10025654	10025458	ID=Msa1009130-mRNA-1:exon:2826;Parent=Msa1009130-mRNA-1
chr7_1	exon	10025751	10025912	10025751	ID=Msa1009130-mRNA-1:exon:2825;Parent=Msa1009130-mRNA-1
chr7_1	exon	10028474	10028815	10028474	ID=Msa1009130-mRNA-1:exon:2824;Parent=Msa1009130-mRNA-1
chr7_1	five_prime_UTR	10028679	10028815	10028679	ID=Msa1009130-mRNA-1:five_prime_utr;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10028474	10028678	10028474	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10025751	10025912	10025751	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10025458	10025654	10025458	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10024741	10024926	10024741	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10024583	10024643	10024583	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	CDS	10023098	10023261	10023098	ID=Msa1009130-mRNA-1:cds;Parent=Msa1009130-mRNA-1
chr7_1	three_prime_UTR	10022853	10023097	10022853	ID=Msa1009130-mRNA-1:three_prime_utr;Parent=Msa1009130-mRNA-1

Gene Sequence

>Msa1009130

ATGAACAACGAGATTTCAGTTATGAAAACAGAGAAAGAAGAAGAACAAGTTCTGAATAATAACAATCTTTCTCCAGCGAGTAACAAAAAACGAAAGAGAAAACTGAGTAAGCAGAGGAAGAAGATGAAGAAGAAATTAGCAACACAACAAAATGATACTAGTTTGAACAGTAACATGATTTCTGAGTTAACTAACTCTGCGCAAGAAGATAGGCCAAAGGATTTGCAAATACCTTCCATTGGAAAATCTGTTGGATGTTTGAAGAAGAAGCTTCTTGTTCTTGATGTTAACGGGCTTCTTGCTGACATAGTTTCACATCCTTACCCAAAGAATGTAAAAAGAGATGCAATGATAGCCAAGAAAGCAGTTTACAAAAGGCCATTTTGTAGCGAGTTTCTGAACTTTTGCTTCGAGAAATTTGATGTGGCTGTGTGGTCCTCAAGAATAGAGTACTCTTCAACTTCAACTTTGTTCTATATAATGTCACTCAACAACTATCTTGTTCTCAATTTGTCTGCATTTATCTTTAAGTTGCTGCCCCTTTTTTCAGGGTCCTATTCTGTGAGCATTTCACAATGTACTGTGACAAATATCAGTACTCTTGAAGACAAACGCAAGACAGTGGTGTTTAAGGATCTGAGAAAAATTTGGGATAAGTCCGATCCGAATCTTCCTTGGGAGAAGGGGTACTACAACGAAACAAATACATTGCTTTTGGATGACTCTCCCTACAAGGGGTTACTTAATCCTCCATACAACTCAATCTTTCCACACTCATTCACTTATCGGAAAAAGAAGGACAAGTCACTAGGTGTTGGAGGTGAACTACGACAATATCTAGAAAAGTTGGCAAAAGTTGAAGATATGGTAAAGTATGTAGAGAAGCACCCACATGGTCAAAATCGTATCAGTGAAACAAGTGAATCTTGGGACTTTTACCTCAAGATTCTTTCTTTGGTTCAACCTAAAGAGTGA

Protein sequence

>Msa1009130

MNNEISVMKTEKEEEQVLNNNNLSPASNKKRKRKLSKQRKKMKKKLATQQNDTSLNSNMISELTNSAQEDRPKDLQIPSIGKSVGCLKKKLLVLDVNGLLADIVSHPYPKNVKRDAMIAKKAVYKRPFCSEFLNFCFEKFDVAVWSSRIEYSSTSTLFYIMSLNNYLVLNLSAFIFKLLPLFSGSYSVSISQCTVTNISTLEDKRKTVVFKDLRKIWDKSDPNLPWEKGYYNETNTLLLDDSPYKGLLNPPYNSIFPHSFTYRKKKDKSLGVGGELRQYLEKLAKVEDMVKYVEKHPHGQNRISETSESWDFYLKILSLVQPKE*