AlfalfaGEDB | Msa1018520

M. sativa cultivar zhongmu-4 / Msa1018520

KEGG: -
GO: -
PFAM: PF14111.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1018520	RHN49141.1	59.353	278	107	2	1	272	1	278	8.08e-114	340

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1018520	A0A396H9C1	59.353	278	107	2	1	272	1	278	3.86e-114	340

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0450000	Msa1018520	0.809052	2.289358e-50	-8.615850e-47
Msa0768290	Msa1018520	0.828713	7.714471e-55	-8.615850e-47
Msa1018520	Msa1025370	0.801849	7.415042e-49	-8.615850e-47
Msa1018520	Msa1121480	0.821450	4.011872e-53	-8.615850e-47
Msa1018520	Msa1121490	0.820794	5.680980e-53	-8.615850e-47
Msa1018520	Msa1248310	0.829669	4.523368e-55	-8.615850e-47
Msa1018520	Msa1289430	0.837249	5.810705e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1018520	MtrunA17_Chr7g0271301	59.353	278	107	2	1	272	1	278	7.43e-118	340
Msa1018520	MtrunA17_Chr1g0178241	50.558	269	115	2	5	255	2	270	1.99e-98	303
Msa1018520	MtrunA17_Chr7g0229881	45.349	258	113	2	5	256	2	237	1.20e-83	255
Msa1018520	MtrunA17_Chr5g0441151	48.131	214	105	3	62	269	13	226	8.13e-68	214
Msa1018520	MtrunA17_Chr7g0235231	48.876	178	88	1	98	272	40	217	1.76e-55	182
Msa1018520	MtrunA17_Chr5g0439091	64.602	113	40	0	121	233	1	113	7.23e-51	162
Msa1018520	MtrunA17_Chr7g0229821	46.622	148	73	1	5	146	2	149	1.41e-46	153
Msa1018520	MtrunA17_Chr1g0171991	48.175	137	71	0	118	254	27	163	1.41e-39	142
Msa1018520	MtrunA17_Chr3g0081461	51.261	119	58	0	45	163	1	119	9.71e-38	129
Msa1018520	MtrunA17_Chr6g0448801	46.429	84	45	0	90	173	6	89	6.94e-19	79.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1018520	AT2G01050.1	27.381	168	118	1	87	254	95	258	4.43e-16	78.2

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Find 82 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAGAAGTTGGAAGGGTTT+TGG	0.286737	7_1:+38536313	Msa1018520:CDS
AAGAGCCTTCGATGAAAGAG+GGG	0.655010	7_1:-38536289	None:intergenic
ACAGCTTGACCAAACGACTT+TGG	0.451744	7_1:-38536111	None:intergenic
ACCCGACGGCGCCGTCTCCG+GGG	0.584980	7_1:-38536088	None:intergenic
ACTCATACTCCACAGCTACC+GGG	0.607310	7_1:-38536728	None:intergenic
ACTGCGCGTGTGACTGAATC+TGG	0.377744	7_1:-38536500	None:intergenic
AGAAAAGGCCAAGTACAAAT+CGG	0.531008	7_1:-38536060	None:intergenic
AGAGCCTTCGATGAAAGAGG+GGG	0.644784	7_1:-38536288	None:intergenic
ATACTCCACAGCTACCGGGA+AGG	0.644839	7_1:-38536724	None:intergenic
ATCTCCCTCAAGAATATTGG+AGG	0.531032	7_1:+38536541	Msa1018520:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAGAAGAATCTCAATATTCT+TGG	-	chr7_1:38536453-38536472	None:intergenic	30.0%
CAGAAGAATCTCAATATTCT+TGG	-	chr7_1:38536453-38536472	None:intergenic	30.0%
AGAAAAGGCCAAGTACAAAT+CGG	-	chr7_1:38536063-38536082	None:intergenic	35.0%
CTCTTAAACAGAAGTTGGAA+GGG	+	chr7_1:38536307-38536326	Msa1018520:CDS	35.0%
TACCTTCTTCATCTCTTCAA+TGG	-	chr7_1:38536403-38536422	None:intergenic	35.0%
TTGAAGAGATGAAGAAGGTA+TGG	+	chr7_1:38536403-38536422	Msa1018520:CDS	35.0%
GAATATTGAGATTCTTCTGC+TGG	+	chr7_1:38536454-38536473	Msa1018520:CDS	35.0%
TGAATCTCCCTCAAGAATAT+TGG	+	chr7_1:38536538-38536557	Msa1018520:CDS	35.0%
TCTTCCTCCAATATTCTTGA+GGG	-	chr7_1:38536548-38536567	None:intergenic	35.0%
AGAAAAGGCCAAGTACAAAT+CGG	-	chr7_1:38536063-38536082	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_1	gene	38536051	38536869	38536051	ID=Msa1018520;Name=Msa1018520
chr7_1	mRNA	38536051	38536869	38536051	ID=Msa1018520-mRNA-1;Parent=Msa1018520;Name=Msa1018520-mRNA-1;_AED=0.26;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|272
chr7_1	exon	38536051	38536869	38536051	ID=Msa1018520-mRNA-1:exon:7470;Parent=Msa1018520-mRNA-1
chr7_1	CDS	38536051	38536869	38536051	ID=Msa1018520-mRNA-1:cds;Parent=Msa1018520-mRNA-1

Gene Sequence

>Msa1018520

ATGGCTACACCGATTTGTACTTGGCCTTTTCTCTCTTCCCCGGAGACGGCGCCGTCGGGTCCAAAGTCGTTTGGTCAAGCTGTGTCTGCTTCTGGGGAAGATTTCTTGCCTTCGCCTCCGCCTAAGGTCATCATCGGTGACACCGTTCGCATTAGAATCAATCAGCGTGAATATGAAGCAGAGCTCTCGGAATGTCTTCGCAACCTCCATGGTCGTGTCACAATGCCGAAGGGCGATCCCCCTCTTTCATCGAAGGCTCTTAAACAGAAGTTGGAAGGGTTTTGGCCACATCTGAAGCCTTGGTCCATCACTCCGCTTGGCAAGGGATACTTCAAATTCAACTTCCACTCCATTGAAGAGATGAAGAAGGTATGGGCATTGGGTGCTGTTCAGTTAAAGCCAAGAATATTGAGATTCTTCTGCTGGTCTAAGGACTTTGATCCTCAAAACCAGATTCAGTCACACGCGCAGTTGTGGGTAAGGCTCATGAATCTCCCTCAAGAATATTGGAGGAAGACCATGCTATATGAAATCGCGTCAGGTATTGGTACCCCCTTCGCTATAGATGAAGCCACACAATCCCGCCTTTTTGGGCACTATGCACGTATTCTCGTGGATGTGGATATGTCCGGTAAGCTGTTTGATTCGGTGGTGGTTGAGAGGGAAGGTTTTGCCTTCCCGGTAGCTGTGGAGTATGAGTGTAAGCCATCATTTTGTTATCACTGTAAGTTGTTAGATCACTCTATTCAGCATTGCAACCGCATTAACAATATACAACATCATGCTGCTCCAAAAAATCAACAGAAGAAACCTATTTAG

Protein sequence

>Msa1018520

MATPICTWPFLSSPETAPSGPKSFGQAVSASGEDFLPSPPPKVIIGDTVRIRINQREYEAELSECLRNLHGRVTMPKGDPPLSSKALKQKLEGFWPHLKPWSITPLGKGYFKFNFHSIEEMKKVWALGAVQLKPRILRFFCWSKDFDPQNQIQSHAQLWVRLMNLPQEYWRKTMLYEIASGIGTPFAIDEATQSRLFGHYARILVDVDMSGKLFDSVVVEREGFAFPVAVEYECKPSFCYHCKLLDHSIQHCNRINNIQHHAAPKNQQKKPI*