AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1031510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031510 MtrunA17_Chr7g0253091 98.750 480 6 0 1 480 1 480 0.0 971
Msa1031510 MtrunA17_Chr6g0461601 77.178 482 107 3 1 479 1 482 0.0 734
Msa1031510 MtrunA17_Chr4g0062771 56.118 474 199 3 6 474 15 484 0.0 543
Msa1031510 MtrunA17_Chr2g0306311 51.093 503 219 3 4 479 8 510 3.18e-178 509
Msa1031510 MtrunA17_Chr8g0383311 54.503 433 194 2 23 454 40 470 1.35e-174 499
Msa1031510 MtrunA17_Chr1g0148391 53.862 479 212 5 1 474 1 475 1.20e-173 496
Msa1031510 MtrunA17_Chr3g0138741 54.430 474 201 6 6 471 15 481 1.80e-169 486
Msa1031510 MtrunA17_Chr3g0129341 50.316 475 228 7 9 479 15 485 5.58e-158 457
Msa1031510 MtrunA17_Chr4g0069801 51.562 448 208 5 25 468 27 469 1.30e-154 448
Msa1031510 MtrunA17_Chr2g0290821 50.345 435 208 5 24 455 26 455 7.54e-154 446
Msa1031510 MtrunA17_Chr4g0076581 48.319 476 232 6 10 477 17 486 2.01e-153 446
Msa1031510 MtrunA17_Chr3g0105981 45.036 413 219 7 24 435 25 430 4.43e-115 345
Msa1031510 MtrunA17_Chr3g0144531 33.941 439 264 18 26 450 28 454 1.13e-61 208
Msa1031510 MtrunA17_Chr1g0161011 32.648 438 266 16 26 450 24 445 1.47e-60 206
Msa1031510 MtrunA17_Chr4g0034901 32.479 468 275 19 8 450 8 459 1.63e-59 202
Msa1031510 MtrunA17_Chr7g0223961 34.529 446 257 20 23 450 20 448 8.82e-58 197
Msa1031510 MtrunA17_Chr5g0439251 32.096 458 250 18 26 444 25 460 2.62e-56 194
Msa1031510 MtrunA17_Chr4g0038941 34.663 326 192 9 24 346 50 357 2.14e-55 188
Msa1031510 MtrunA17_Chr4g0038981 34.421 337 202 9 9 342 4 324 5.02e-55 187
Msa1031510 MtrunA17_Chr8g0389741 29.524 420 274 13 26 434 28 436 2.41e-54 189
Msa1031510 MtrunA17_Chr6g0487261 30.719 459 278 18 6 442 11 451 7.71e-54 188
Msa1031510 MtrunA17_Chr4g0039011 35.077 325 193 9 26 348 42 350 8.37e-54 184
Msa1031510 MtrunA17_Chr4g0038961 33.234 337 206 9 9 342 4 324 1.91e-53 182
Msa1031510 MtrunA17_Chr1g0189111 34.731 334 197 11 23 347 28 349 2.45e-53 185
Msa1031510 MtrunA17_Chr4g0038971 32.164 342 213 9 9 347 4 329 8.13e-53 181
Msa1031510 MtrunA17_Chr1g0177171 30.258 466 271 19 26 450 38 490 1.19e-52 185
Msa1031510 MtrunA17_Chr4g0046311 30.543 442 271 19 26 444 23 451 1.23e-52 184
Msa1031510 MtrunA17_Chr3g0110371 30.023 443 271 16 26 450 24 445 1.34e-52 184
Msa1031510 MtrunA17_Chr2g0294601 31.844 358 227 7 7 357 35 382 4.29e-52 181
Msa1031510 MtrunA17_Chr1g0146831 35.549 346 194 15 10 340 16 347 1.37e-51 179
Msa1031510 MtrunA17_Chr4g0003281 34.940 332 195 12 27 348 41 361 7.28e-51 177
Msa1031510 MtrunA17_Chr4g0043511 29.211 469 284 16 26 464 62 512 5.18e-50 179
Msa1031510 MtrunA17_Chr2g0294591 33.131 329 204 7 26 350 30 346 1.50e-49 173
Msa1031510 MtrunA17_Chr5g0434591 29.452 438 280 16 26 450 23 444 2.25e-49 176
Msa1031510 MtrunA17_Chr8g0378621 29.299 471 284 19 3 450 4 448 4.41e-49 175
Msa1031510 MtrunA17_Chr4g0064021 32.530 332 203 11 27 348 27 347 7.90e-49 172
Msa1031510 MtrunA17_Chr7g0250301 32.407 324 199 11 26 344 8 316 2.09e-48 169
Msa1031510 MtrunA17_Chr3g0119541 34.969 326 187 12 24 337 24 336 2.24e-48 170
Msa1031510 MtrunA17_Chr3g0121271 28.817 465 288 19 3 448 4 444 2.84e-48 173
Msa1031510 MtrunA17_Chr8g0339191 29.478 441 282 17 27 450 30 458 4.39e-48 172
Msa1031510 MtrunA17_Chr4g0075031 29.318 440 278 15 26 450 45 466 7.49e-48 172
Msa1031510 MtrunA17_Chr4g0039071 32.121 330 207 9 24 350 23 338 1.92e-47 168
Msa1031510 MtrunA17_Chr4g0043851 32.303 356 214 12 9 348 8 352 8.61e-47 167
Msa1031510 MtrunA17_Chr4g0026321 31.373 357 221 15 25 370 28 371 2.73e-46 165
Msa1031510 MtrunA17_Chr4g0039021 33.444 302 182 10 46 342 1 288 9.42e-45 158
Msa1031510 MtrunA17_Chr2g0321511 31.832 333 205 13 27 350 30 349 2.72e-44 160
Msa1031510 MtrunA17_Chr1g0174261 33.219 292 179 9 55 342 1 280 6.69e-44 157
Msa1031510 MtrunA17_Chr1g0155001 32.036 334 207 11 26 349 24 347 5.39e-43 157
Msa1031510 MtrunA17_Chr2g0285901 32.812 320 194 11 27 337 25 332 3.11e-42 154
Msa1031510 MtrunA17_Chr7g0234821 27.088 443 293 18 27 450 27 458 5.29e-41 152
Msa1031510 MtrunA17_Chr7g0250321 31.834 289 180 9 3 288 7 281 1.75e-37 139
Msa1031510 MtrunA17_Chr5g0436361 27.778 324 212 13 43 357 54 364 1.70e-25 107
Msa1031510 MtrunA17_Chr2g0294581 36.567 134 82 2 26 158 76 207 3.03e-18 85.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031510 AT1G64760.1 74.667 450 110 2 6 455 8 453 0.0 690
Msa1031510 AT1G64760.2 74.667 450 110 2 6 455 8 453 0.0 690
Msa1031510 AT2G19440.2 74.000 450 114 2 6 455 3 449 0.0 688
Msa1031510 AT2G19440.1 74.000 450 114 2 6 455 3 449 0.0 688
Msa1031510 AT3G04010.1 68.151 449 141 2 23 470 30 477 0.0 631
Msa1031510 AT5G18220.1 66.245 474 148 5 9 477 13 479 0.0 627
Msa1031510 AT3G24330.1 52.101 476 225 3 6 479 19 493 1.89e-175 502
Msa1031510 AT5G64790.1 55.727 454 191 3 6 458 15 459 2.08e-174 499
Msa1031510 AT4G31140.1 53.416 483 211 7 6 479 6 483 1.82e-173 496
Msa1031510 AT5G58090.1 53.275 458 207 5 20 473 16 470 7.00e-168 482
Msa1031510 AT5G20870.1 52.489 442 195 6 23 454 27 463 3.77e-150 438
Msa1031510 AT4G17180.1 48.238 454 222 5 10 461 9 451 7.53e-147 428
Msa1031510 AT5G58480.1 44.295 447 237 7 23 466 23 460 3.99e-124 370
Msa1031510 AT5G55180.2 33.871 434 261 16 19 436 19 442 1.33e-66 221
Msa1031510 AT5G55180.1 33.259 448 273 16 19 450 19 456 1.41e-66 221
Msa1031510 AT2G16230.1 33.790 438 264 16 26 450 25 449 3.22e-65 219
Msa1031510 AT2G05790.1 33.333 462 253 21 26 450 25 468 6.85e-64 214
Msa1031510 AT3G55430.1 34.240 441 260 17 23 450 26 449 3.91e-63 212
Msa1031510 AT2G39640.1 32.522 452 269 17 9 448 13 440 3.29e-60 207
Msa1031510 AT4G26830.1 31.579 456 284 16 10 450 9 451 1.54e-59 202
Msa1031510 AT4G26830.2 32.273 440 270 17 26 450 29 455 7.80e-59 201
Msa1031510 AT2G01630.1 31.111 450 274 17 26 461 23 450 6.81e-58 199
Msa1031510 AT2G01630.3 30.716 433 269 16 26 444 23 438 1.31e-56 194
Msa1031510 AT4G34480.1 33.030 439 263 17 26 450 26 447 1.72e-56 196
Msa1031510 AT3G13560.4 33.108 444 267 15 20 450 20 446 2.98e-56 195
Msa1031510 AT3G13560.1 33.108 444 267 15 20 450 20 446 2.98e-56 195
Msa1031510 AT3G13560.2 33.108 444 267 15 20 450 20 446 2.98e-56 195
Msa1031510 AT3G13560.5 33.108 444 267 15 20 450 20 446 2.98e-56 195
Msa1031510 AT3G13560.3 33.108 444 267 15 20 450 20 446 2.98e-56 195
Msa1031510 AT3G07320.1 32.200 441 271 18 27 450 26 455 3.58e-56 194
Msa1031510 AT1G66250.1 30.543 442 269 15 26 450 32 452 1.05e-55 194
Msa1031510 AT5G24318.1 29.122 467 287 19 7 450 10 455 1.95e-54 189
Msa1031510 AT5G24318.2 29.322 457 279 19 7 440 10 445 3.96e-54 188
Msa1031510 AT1G11820.2 30.023 443 271 16 26 450 44 465 1.36e-53 188
Msa1031510 AT4G14080.1 31.236 445 273 20 26 450 42 473 1.89e-53 187
Msa1031510 AT4G16260.1 32.722 327 206 7 24 348 22 336 2.39e-53 183
Msa1031510 AT1G11820.3 29.977 437 267 16 26 444 44 459 2.10e-52 184
Msa1031510 AT3G23770.1 31.696 448 270 21 26 450 37 471 3.83e-52 183
Msa1031510 AT4G16260.2 33.228 316 196 8 36 348 4 307 4.90e-52 179
Msa1031510 AT3G57270.1 33.951 324 200 9 26 346 27 339 5.61e-51 177
Msa1031510 AT5G56590.1 29.075 454 281 17 20 451 20 454 9.62e-51 180
Msa1031510 AT3G57260.1 32.558 344 212 9 6 346 12 338 2.18e-50 175
Msa1031510 AT3G57260.2 32.558 344 212 9 6 346 28 354 2.73e-50 175
Msa1031510 AT5G42720.1 34.513 339 201 12 11 337 8 337 1.24e-49 176
Msa1031510 AT2G26600.1 31.549 355 219 12 22 363 30 373 1.28e-49 174
Msa1031510 AT1G30080.2 32.464 345 211 11 4 337 12 345 1.63e-49 174
Msa1031510 AT1G30080.1 32.464 345 211 11 4 337 12 345 2.41e-49 174
Msa1031510 AT3G15800.1 32.840 338 205 12 22 348 39 365 4.44e-48 171
Msa1031510 AT5G42100.2 35.119 336 195 13 23 346 24 348 1.34e-47 170
Msa1031510 AT5G42100.1 35.119 336 195 13 23 346 24 348 1.55e-47 170
Msa1031510 AT4G34480.2 36.533 323 182 14 26 337 26 336 2.81e-47 167
Msa1031510 AT1G32860.1 33.538 325 192 12 26 338 29 341 1.57e-46 167
Msa1031510 AT2G01630.2 31.138 334 209 11 26 348 23 346 9.16e-46 164
Msa1031510 AT5G24318.4 30.460 348 215 13 7 342 10 342 3.76e-45 162
Msa1031510 AT4G29360.1 26.294 483 306 16 5 452 10 477 1.95e-44 164
Msa1031510 AT4G18340.2 31.471 340 211 12 9 338 17 344 4.31e-44 159
Msa1031510 AT3G57240.1 31.790 324 198 11 23 342 32 336 5.89e-44 158
Msa1031510 AT4G18340.1 31.176 340 212 12 9 338 17 344 8.68e-44 159
Msa1031510 AT4G29360.2 26.407 462 294 15 26 452 37 487 2.23e-43 161
Msa1031510 AT1G11820.1 29.940 334 215 10 26 349 44 368 5.64e-42 154
Msa1031510 AT3G61810.1 28.869 336 207 12 26 347 55 372 6.75e-40 148
Msa1031510 AT2G27500.2 29.377 337 217 11 24 350 27 352 1.26e-39 147
Msa1031510 AT2G27500.1 28.933 356 230 13 24 368 27 370 2.57e-39 147
Msa1031510 AT4G34480.3 32.665 349 207 15 115 450 3 336 4.92e-39 146
Msa1031510 AT3G46570.1 30.117 342 215 15 11 342 13 340 3.96e-37 140
Msa1031510 AT1G77780.1 29.412 323 208 9 27 344 25 332 1.82e-34 133
Msa1031510 AT2G26600.2 30.714 280 171 11 96 363 11 279 2.29e-34 130
Msa1031510 AT5G24318.3 29.641 334 196 15 138 450 1 316 1.30e-33 129
Msa1031510 AT5G20390.1 28.834 326 210 11 26 344 33 343 1.32e-32 127
Msa1031510 AT1G77790.1 28.530 347 222 13 7 342 8 339 6.25e-31 122
Msa1031510 AT5G20330.1 26.686 341 218 11 11 334 8 333 3.88e-30 120
Msa1031510 AT5G20340.1 27.329 322 213 10 19 334 36 342 4.51e-30 120
Msa1031510 AT3G55780.1 26.000 350 229 12 26 357 31 368 9.08e-30 120
Msa1031510 AT1G33220.1 27.597 308 205 9 19 320 27 322 4.45e-29 117
Msa1031510 AT2G27500.3 29.123 285 181 11 76 350 1 274 5.27e-29 116
Msa1031510 AT5G20560.1 26.686 341 224 11 6 334 8 334 4.27e-28 114

Find 132 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAGACATCTGCGTTTA+AGG 0.135341 7_1:-66222234 None:intergenic
CATCAAAGACGTTGGTATAT+TGG 0.201622 7_1:-66222466 None:intergenic
ATTATAAACGTGCTCAATAT+TGG 0.203608 7_1:+66220596 Msa1031510:CDS
AACATCTTCACAACAGTTTC+TGG 0.211606 7_1:-66220448 None:intergenic
TGGATTCCATTGTCAGTTAT+TGG 0.212683 7_1:-66222446 None:intergenic
GAGAGAGAAGGGAACGGTTT+TGG 0.216296 7_1:-66220232 None:intergenic
ATTTCAGCAAGTTGATTATT+AGG 0.225555 7_1:-66220568 None:intergenic
GTTGTTGATGTTAGGATTAT+TGG 0.250047 7_1:+66220357 Msa1031510:CDS
TGGAAGGGCTTGGTGTGAAC+TGG 0.270727 7_1:+66220398 Msa1031510:CDS
CCTACTTCTCCTACCAAAAT+TGG 0.271582 7_1:-66222542 None:intergenic
TGCAACAACCTTGATGCTAA+TGG 0.283164 7_1:+66222965 Msa1031510:CDS
TTTCGGCGACACGCCAATTT+TGG 0.288643 7_1:+66222529 Msa1031510:CDS
AAGTTTCCGATGGATCTTTC+GGG 0.294935 7_1:+66222779 Msa1031510:CDS
GCTCTCAAATCGATCGGTTT+CGG 0.295941 7_1:+66222512 Msa1031510:CDS
TAAGGGTACTGTTGCCTTTA+CGG 0.296654 7_1:-66222216 None:intergenic
ATTAACTTTGCTTGCACCTT+TGG 0.307246 7_1:+66222911 Msa1031510:CDS
TTCATCGAAGTTTATCTGTT+TGG 0.307651 7_1:+66222674 Msa1031510:CDS
TCTTATTGCATTGCCGGAAT+TGG 0.311120 7_1:-66222591 None:intergenic
GTAGAATCTTATTGCATTGC+CGG 0.319258 7_1:-66222597 None:intergenic
GGGTAAATGTTGACAGTAAA+TGG 0.333092 7_1:-66222356 None:intergenic
TTCCATCTGCGGGAATATTT+CGG 0.334659 7_1:+66222276 Msa1031510:CDS
TTTACAATGTTGTTGATGTT+AGG 0.335818 7_1:+66220349 Msa1031510:CDS
AGACGGTGATAGTGATGAAA+TGG 0.342594 7_1:-66220140 None:intergenic
CAGATAAACTTCGATGAATC+CGG 0.350042 7_1:-66222669 None:intergenic
TTATAAACGTGCTCAATATT+GGG 0.352564 7_1:+66220597 Msa1031510:CDS
GTGAAGATGTTGAAGGATAA+TGG 0.352574 7_1:+66220460 Msa1031510:CDS
GTTACTCGTTATAACTTTGA+TGG 0.354819 7_1:+66220631 Msa1031510:CDS
TTGTTGATGTTAGGATTATT+GGG 0.376590 7_1:+66220358 Msa1031510:CDS
GGTGACAAGAATGCCAATTC+CGG 0.398140 7_1:+66222578 Msa1031510:CDS
GAAGTTTCCGATGGATCTTT+CGG 0.401121 7_1:+66222778 Msa1031510:CDS
TTGCTGAGATCTTTAGCATC+AGG 0.401772 7_1:-66222875 None:intergenic
TTGTGAAGTTAGAGAGAGAA+GGG 0.406470 7_1:-66220243 None:intergenic
TGTTATTATGTGGAAGGGCT+TGG 0.408056 7_1:+66220388 Msa1031510:CDS
TCGAAGAAGGCGAAGTTGAA+TGG 0.408365 7_1:-66222410 None:intergenic
AGTCATGAGACCATTGATAT+CGG 0.412963 7_1:-66222300 None:intergenic
TCAACTTCGCCTTCTTCGAC+GGG 0.422981 7_1:+66222414 Msa1031510:CDS
TGAAGATGTTGAAGGATAAT+GGG 0.423140 7_1:+66220461 Msa1031510:CDS
CGATGAATCCGGGGCGGAGA+GGG 0.424811 7_1:-66222658 None:intergenic
TTCAACTTCGCCTTCTTCGA+CGG 0.430662 7_1:+66222413 Msa1031510:CDS
GACGATCCTGATTCTGCGTC+TGG 0.432976 7_1:-66223018 None:intergenic
GTACTACCAGACGCAGAATC+AGG 0.436349 7_1:+66223012 Msa1031510:CDS
ACCAAGACCAGCTTCATTGA+TGG 0.438757 7_1:-66222189 None:intergenic
TTTATCTACCCCGTCGAAGA+AGG 0.451188 7_1:-66222423 None:intergenic
TCCGCAGATATGTAGCGGTT+GGG 0.453302 7_1:+66222090 Msa1031510:intron
TACCCCTTCTTAAGTCTCTA+CGG 0.454310 7_1:+66222374 Msa1031510:CDS
ATGCTGAAGAAGGAACTATC+TGG 0.458076 7_1:-66223114 None:intergenic
AACGGAGAGGCTAAGGAGTA+AGG 0.458554 7_1:-66220007 None:intergenic
AGAGAGAAACGGAGAGGCTA+AGG 0.461912 7_1:-66220014 None:intergenic
CTTCAAAACAAACTTCTAGT+AGG 0.464131 7_1:+66222806 Msa1031510:CDS
GCATTCTTGTCACCTTCTGT+TGG 0.465889 7_1:-66222569 None:intergenic
GGAAGGGCTTGGTGTGAACT+GGG 0.467914 7_1:+66220399 Msa1031510:CDS
GGTTTCAACATTTATTTGAC+CGG 0.468679 7_1:+66223243 Msa1031510:three_prime_UTR
AAGCATTTCCATTAGCATCA+AGG 0.472386 7_1:-66222973 None:intergenic
CGAAGAAGGCGAAGTTGAAT+GGG 0.474909 7_1:-66222409 None:intergenic
CAATCAAACAAACCAATTAG+AGG 0.475078 7_1:+66220045 Msa1031510:five_prime_UTR
TGATACCACAATGACTGCTT+TGG 0.475443 7_1:+66220513 Msa1031510:CDS
GACGGTGATAGTGATGAAAT+GGG 0.478927 7_1:-66220139 None:intergenic
TTCCGCAGATATGTAGCGGT+TGG 0.484860 7_1:+66222089 Msa1031510:intron
ACCCCTTCTTAAGTCTCTAC+GGG 0.484918 7_1:+66222375 Msa1031510:CDS
CAGTGTCTCTCAAAGTTACC+TGG 0.486453 7_1:-66222728 None:intergenic
GATTGTACTGCACTTGGGTA+TGG 0.490793 7_1:+66222935 Msa1031510:CDS
TGATGCAAATTTCGATACAT+TGG 0.491804 7_1:+66222484 Msa1031510:CDS
TGGGGAATATTTCGATATGA+TGG 0.495745 7_1:+66222749 Msa1031510:CDS
TGTTCGCAGCGAGTCTAGAA+AGG 0.496041 7_1:-66222628 None:intergenic
GCCATCAATGAAGCTGGTCT+TGG 0.496624 7_1:+66222188 Msa1031510:CDS
AACGTGCTCAATATTGGGTT+AGG 0.498245 7_1:+66220602 Msa1031510:CDS
GTTGAAATGTTGTCTGTAGT+AGG 0.500081 7_1:-66223070 None:intergenic
ATGACTGCTTTGGCTGGTAG+TGG 0.501400 7_1:+66220523 Msa1031510:CDS
TGGTAGTGGAATTGAAGTTA+TGG 0.502034 7_1:+66220537 Msa1031510:CDS
TAAATAAACTAGATATCATC+CGG 0.514578 7_1:-66223262 None:intergenic
TTTGGAGATTGTACTGCACT+TGG 0.515034 7_1:+66222929 Msa1031510:CDS
TTGTTTCTGTCAGAGAGAAA+CGG 0.516816 7_1:-66220025 None:intergenic
GTCATGAGACCATTGATATC+GGG 0.521177 7_1:-66222299 None:intergenic
GTTTCTGCTCTCAAATCGAT+CGG 0.522943 7_1:+66222506 Msa1031510:CDS
CAAAATGCCATCAATGAAGC+TGG 0.527897 7_1:+66222182 Msa1031510:CDS
ACCACAATGACTGCTTTGGC+TGG 0.529623 7_1:+66220517 Msa1031510:CDS
ATCAAAGAAGATGCTGAAGA+AGG 0.530104 7_1:-66223124 None:intergenic
AGATAAACTTCGATGAATCC+GGG 0.532252 7_1:-66222668 None:intergenic
TTTGTGAAGTTAGAGAGAGA+AGG 0.537861 7_1:-66220244 None:intergenic
TGCTGAGATCTTTAGCATCA+GGG 0.538134 7_1:-66222874 None:intergenic
CAAACTTCTAGTAGGTGCAC+AGG 0.538210 7_1:+66222814 Msa1031510:CDS
ATATAGACATCTGCGTTTAA+GGG 0.538392 7_1:-66222233 None:intergenic
CAGGTAACTTTGAGAGACAC+TGG 0.539845 7_1:+66222729 Msa1031510:CDS
GACAATCAAGTTCCATCTGC+GGG 0.543282 7_1:+66222266 Msa1031510:CDS
GATGAATCCGGGGCGGAGAG+GGG 0.546532 7_1:-66222657 None:intergenic
GACGCGAAGAGCATTGCTCC+AGG 0.552654 7_1:+66222710 Msa1031510:CDS
ATATTTCGGCCCGATATCAA+TGG 0.563489 7_1:+66222290 Msa1031510:CDS
TCGATGAATCCGGGGCGGAG+AGG 0.565244 7_1:-66222659 None:intergenic
AAGTTAGAGAGAGAAGGGAA+CGG 0.565537 7_1:-66220238 None:intergenic
AAACAACAAAGCAGCAAACG+AGG 0.567675 7_1:-66223149 None:intergenic
TTGGAGATTGTACTGCACTT+GGG 0.571116 7_1:+66222930 Msa1031510:CDS
AACTGTTGTGAAGATGTTGA+AGG 0.573217 7_1:+66220453 Msa1031510:CDS
GATAAACCAATAACTGACAA+TGG 0.575139 7_1:+66222440 Msa1031510:CDS
TCTAGACTCGCTGCGAACAA+AGG 0.575607 7_1:+66222632 Msa1031510:CDS
AAAGGAACCCCTCTCCGCCC+CGG 0.576993 7_1:+66222650 Msa1031510:CDS
GAAAGATCCATCGGAAACTT+CGG 0.579080 7_1:-66222776 None:intergenic
ATCACCGTCTTATTCATCGT+GGG 0.579244 7_1:+66220153 Msa1031510:five_prime_UTR
GAAGTAGGATGGCCAACAGA+AGG 0.581640 7_1:+66222557 Msa1031510:CDS
TGGTCTTGGTGACACCGTAA+AGG 0.581918 7_1:+66222202 Msa1031510:CDS
AATGTTCTGCAGTGCAGGAA+AGG 0.584113 7_1:-66222159 None:intergenic
CAATGGTCTCATGACTCAGA+TGG 0.589085 7_1:+66222307 Msa1031510:CDS
GAAATTTGCATCAAAGACGT+TGG 0.589177 7_1:-66222474 None:intergenic
AGGTAACTTTGAGAGACACT+GGG 0.591795 7_1:+66222730 Msa1031510:CDS
GTTCCCGTAGAGACTTAAGA+AGG 0.603438 7_1:-66222378 None:intergenic
AGATGTCTATATGTCTCCGC+AGG 0.611737 7_1:+66222244 Msa1031510:CDS
GAAGGGCTTGGTGTGAACTG+GGG 0.611743 7_1:+66220400 Msa1031510:CDS
ACTCGTTATAACTTTGATGG+CGG 0.617236 7_1:+66220634 Msa1031510:CDS
GGGTTAAACATGCACCACAT+TGG 0.618943 7_1:-66222854 None:intergenic
TATCACCGTCTTATTCATCG+TGG 0.622354 7_1:+66220152 Msa1031510:five_prime_UTR
TGGATGATAAAATTGCAAGT+AGG 0.623501 7_1:-66223094 None:intergenic
TTCCCGTAGAGACTTAAGAA+GGG 0.632332 7_1:-66222377 None:intergenic
GTACAATTTCTCAACAAGAA+CGG 0.637978 7_1:+66222329 Msa1031510:CDS
ATCTTCACAACAGTTTCTGG+TGG 0.639053 7_1:-66220445 None:intergenic
ACCAGCCAAAGCAGTCATTG+TGG 0.643674 7_1:-66220518 None:intergenic
AGTTGACCCGAAAGATCCAT+CGG 0.649595 7_1:-66222785 None:intergenic
TCCCGTAGAGACTTAAGAAG+GGG 0.652260 7_1:-66222376 None:intergenic
GGACAATCAAGTTCCATCTG+CGG 0.652978 7_1:+66222265 Msa1031510:CDS
AAGTGCAGTACAATCTCCAA+AGG 0.655496 7_1:-66222927 None:intergenic
TGGACAACCGAAGTTTCCGA+TGG 0.662375 7_1:+66222769 Msa1031510:CDS
AACAACAAAGCAGCAAACGA+GGG 0.662636 7_1:-66223148 None:intergenic
AAACTTCTAGTAGGTGCACA+GGG 0.662815 7_1:+66222815 Msa1031510:CDS
TCTGTTTGGACTCATCGATG+AGG 0.680701 7_1:+66222688 Msa1031510:CDS
TCGCAGCGAGTCTAGAAAGG+AGG 0.683390 7_1:-66222625 None:intergenic
GATAAACTTCGATGAATCCG+GGG 0.689389 7_1:-66222667 None:intergenic
AATGCAATAAGATTCTACAA+TGG 0.690889 7_1:+66222602 Msa1031510:CDS
TGAAATATCTTGATCCAATG+TGG 0.694528 7_1:+66222840 Msa1031510:CDS
CAACTTCGCCTTCTTCGACG+GGG 0.696178 7_1:+66222415 Msa1031510:CDS
GATGGAACTTGATTGTCCTG+CGG 0.701807 7_1:-66222260 None:intergenic
GAAACCCACGATGAATAAGA+CGG 0.707131 7_1:-66220157 None:intergenic
TCTGTCAGAGAGAAACGGAG+AGG 0.734759 7_1:-66220020 None:intergenic
AAACTTCGATGAATCCGGGG+CGG 0.742113 7_1:-66222664 None:intergenic
GGTAACTTTGAGAGACACTG+GGG 0.786507 7_1:+66222731 Msa1031510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTTATTTTTGTC+AGG + chr7_1:66220976-66220995 Msa1031510:intron 10.0%
!!! TTTTATTTTTATTTTTGTCA+GGG + chr7_1:66220977-66220996 Msa1031510:intron 10.0%
!!! ATTTTTATTCGTTGAGTTTA+GGG + chr7_1:66219972-66219991 Msa1031510:five_prime_UTR 20.0%
!!! TTTGTTAGATTTTTGATTCA+TGG + chr7_1:66220942-66220961 Msa1031510:intron 20.0%
!! ATCATTATTATGAACACAAA+GGG - chr7_1:66221033-66221052 None:intergenic 20.0%
!! AATCATTATTATGAACACAA+AGG - chr7_1:66221034-66221053 None:intergenic 20.0%
!! ATAAAAGAATCTAATTCTGA+GGG - chr7_1:66221185-66221204 None:intergenic 20.0%
!! AATAAAAGAATCTAATTCTG+AGG - chr7_1:66221186-66221205 None:intergenic 20.0%
!!! TCAGAATTAGATTCTTTTAT+TGG + chr7_1:66221185-66221204 Msa1031510:intron 20.0%
!!! GATTTTATTGAACATTTTGA+AGG + chr7_1:66221716-66221735 Msa1031510:intron 20.0%
!! TTATTGTAAGCTTTCAAAAA+TGG - chr7_1:66222122-66222141 None:intergenic 20.0%
!! TAAATAAACTAGATATCATC+CGG - chr7_1:66223265-66223284 None:intergenic 20.0%
!! GATTTTTATTCGTTGAGTTT+AGG + chr7_1:66219971-66219990 Msa1031510:five_prime_UTR 25.0%
!! AAAATGAAGAAGTTTTTGCT+GGG - chr7_1:66220302-66220321 None:intergenic 25.0%
! ATCTCACAATGGAAAAAAAT+TGG + chr7_1:66220323-66220342 Msa1031510:exon 25.0%
! TTTACAATGTTGTTGATGTT+AGG + chr7_1:66220349-66220368 Msa1031510:CDS 25.0%
! TTGTTGATGTTAGGATTATT+GGG + chr7_1:66220358-66220377 Msa1031510:CDS 25.0%
!!! TGTTTTTGTTATTATGTGGA+AGG + chr7_1:66220382-66220401 Msa1031510:CDS 25.0%
!!! GTTTTTGTTATTATGTGGAA+GGG + chr7_1:66220383-66220402 Msa1031510:CDS 25.0%
!!! ATTTCAGCAAGTTGATTATT+AGG - chr7_1:66220571-66220590 None:intergenic 25.0%
! ATTATAAACGTGCTCAATAT+TGG + chr7_1:66220596-66220615 Msa1031510:CDS 25.0%
! TTATAAACGTGCTCAATATT+GGG + chr7_1:66220597-66220616 Msa1031510:CDS 25.0%
!!! AATTTTTCGAGATTTTGTGT+TGG + chr7_1:66220777-66220796 Msa1031510:intron 25.0%
!!! TAGATTTTTGATTCATGGTT+AGG + chr7_1:66220947-66220966 Msa1031510:intron 25.0%
!!! TGATTATGCATTTTGATTGA+TGG + chr7_1:66221055-66221074 Msa1031510:intron 25.0%
!!! AGTTGTTCTTGTTTTAATCA+AGG + chr7_1:66221137-66221156 Msa1031510:intron 25.0%
!! TTAGATTCTTTTATTGGTGT+TGG + chr7_1:66221191-66221210 Msa1031510:intron 25.0%
!!! TTCCTTGTAGTAACTTTTTT+CGG + chr7_1:66221225-66221244 Msa1031510:intron 25.0%
! AGAACAAATACATTATACCT+CGG - chr7_1:66221253-66221272 None:intergenic 25.0%
! ATGTGCATATATCATATTCT+TGG + chr7_1:66221351-66221370 Msa1031510:intron 25.0%
!! TTTTCCTTCTGTATTATAGT+TGG + chr7_1:66221517-66221536 Msa1031510:intron 25.0%
!! GTCTGTTCAAACAATTTTAA+AGG + chr7_1:66221550-66221569 Msa1031510:intron 25.0%
!! TATTTTTCACAATCAGCAAT+TGG - chr7_1:66221638-66221657 None:intergenic 25.0%
! TTTGACAACACTAGATTTAA+TGG + chr7_1:66221693-66221712 Msa1031510:intron 25.0%
! TTGACAACACTAGATTTAAT+GGG + chr7_1:66221694-66221713 Msa1031510:intron 25.0%
! TGGTGTTTAATAATAAGAAG+CGG - chr7_1:66221747-66221766 None:intergenic 25.0%
! ATTCATCAAAGCTTAAAATG+TGG - chr7_1:66221767-66221786 None:intergenic 25.0%
! GCACAAATTCACATTAAAAT+CGG - chr7_1:66222054-66222073 None:intergenic 25.0%
! AATGCAATAAGATTCTACAA+TGG + chr7_1:66222602-66222621 Msa1031510:CDS 25.0%
CAATCAAACAAACCAATTAG+AGG + chr7_1:66220045-66220064 Msa1031510:five_prime_UTR 30.0%
! GTTTCTTTTTGACCTCTAAT+TGG - chr7_1:66220060-66220079 None:intergenic 30.0%
! GAAAATGAAGAAGTTTTTGC+TGG - chr7_1:66220303-66220322 None:intergenic 30.0%
! TCTTCATTTTCATCTCACAA+TGG + chr7_1:66220312-66220331 Msa1031510:exon 30.0%
GTTGTTGATGTTAGGATTAT+TGG + chr7_1:66220357-66220376 Msa1031510:CDS 30.0%
!!! GGGTTGTTTTTGTTATTATG+TGG + chr7_1:66220378-66220397 Msa1031510:CDS 30.0%
TGAAGATGTTGAAGGATAAT+GGG + chr7_1:66220461-66220480 Msa1031510:CDS 30.0%
GTTACTCGTTATAACTTTGA+TGG + chr7_1:66220631-66220650 Msa1031510:CDS 30.0%
! GAGAATCATTTTACCATTCT+AGG + chr7_1:66221273-66221292 Msa1031510:intron 30.0%
GCATATATCATATTCTTGGA+AGG + chr7_1:66221355-66221374 Msa1031510:intron 30.0%
TGATTGAGAGTTCATATAAC+AGG + chr7_1:66221433-66221452 Msa1031510:intron 30.0%
! TAACAGGAAATTTTGCATCA+AGG + chr7_1:66221449-66221468 Msa1031510:intron 30.0%
TCAACCAACTATAATACAGA+AGG - chr7_1:66221524-66221543 None:intergenic 30.0%
! GCTTGTAGAGATTTTATTGA+TGG + chr7_1:66221585-66221604 Msa1031510:intron 30.0%
AAATCTAGTGTTGTCAAAAG+CGG - chr7_1:66221691-66221710 None:intergenic 30.0%
! TAGCGAATAATTTGTAATGC+TGG + chr7_1:66221872-66221891 Msa1031510:intron 30.0%
!! AAGATGGCATTGATGAATTT+GGG - chr7_1:66221971-66221990 None:intergenic 30.0%
!! AAAGATGGCATTGATGAATT+TGG - chr7_1:66221972-66221991 None:intergenic 30.0%
CATTAAAATCGGTCGAAATT+CGG - chr7_1:66222043-66222062 None:intergenic 30.0%
ATATAGACATCTGCGTTTAA+GGG - chr7_1:66222236-66222255 None:intergenic 30.0%
GTACAATTTCTCAACAAGAA+CGG + chr7_1:66222329-66222348 Msa1031510:CDS 30.0%
GATAAACCAATAACTGACAA+TGG + chr7_1:66222440-66222459 Msa1031510:CDS 30.0%
TGATGCAAATTTCGATACAT+TGG + chr7_1:66222484-66222503 Msa1031510:CDS 30.0%
TTCATCGAAGTTTATCTGTT+TGG + chr7_1:66222674-66222693 Msa1031510:CDS 30.0%
CTTCAAAACAAACTTCTAGT+AGG + chr7_1:66222806-66222825 Msa1031510:CDS 30.0%
TGAAATATCTTGATCCAATG+TGG + chr7_1:66222840-66222859 Msa1031510:CDS 30.0%
TGGATGATAAAATTGCAAGT+AGG - chr7_1:66223097-66223116 None:intergenic 30.0%
!!! TGCTTTTTCTGTGATTCTAT+TGG + chr7_1:66223174-66223193 Msa1031510:CDS 30.0%
GGTTTCAACATTTATTTGAC+CGG + chr7_1:66223243-66223262 Msa1031510:three_prime_UTR 30.0%
!! TTGTTTCTGTCAGAGAGAAA+CGG - chr7_1:66220028-66220047 None:intergenic 35.0%
TTGTGAAGTTAGAGAGAGAA+GGG - chr7_1:66220246-66220265 None:intergenic 35.0%
TTTGTGAAGTTAGAGAGAGA+AGG - chr7_1:66220247-66220266 None:intergenic 35.0%
AACATCTTCACAACAGTTTC+TGG - chr7_1:66220451-66220470 None:intergenic 35.0%
AACTGTTGTGAAGATGTTGA+AGG + chr7_1:66220453-66220472 Msa1031510:CDS 35.0%
GTGAAGATGTTGAAGGATAA+TGG + chr7_1:66220460-66220479 Msa1031510:CDS 35.0%
!! TGGTAGTGGAATTGAAGTTA+TGG + chr7_1:66220537-66220556 Msa1031510:CDS 35.0%
ACTCGTTATAACTTTGATGG+CGG + chr7_1:66220634-66220653 Msa1031510:CDS 35.0%
TGAAGCAAGCACATTATACA+AGG - chr7_1:66220821-66220840 None:intergenic 35.0%
GTATAATGTGCTTGCTTCAT+TGG + chr7_1:66220822-66220841 Msa1031510:intron 35.0%
!!! TTTTGATTGATGGCGAGTTT+TGG + chr7_1:66221065-66221084 Msa1031510:intron 35.0%
!!! TTTGATTGATGGCGAGTTTT+GGG + chr7_1:66221066-66221085 Msa1031510:intron 35.0%
! ATCAAGGAAGCTTTATGTAC+CGG + chr7_1:66221153-66221172 Msa1031510:intron 35.0%
CACCGAAAAAAGTTACTACA+AGG - chr7_1:66221230-66221249 None:intergenic 35.0%
!! TAGGAGTTGATTGCAGTTTT+AGG + chr7_1:66221292-66221311 Msa1031510:intron 35.0%
!! AGGAGTTGATTGCAGTTTTA+GGG + chr7_1:66221293-66221312 Msa1031510:intron 35.0%
CCAATCCAATGACAAAAACA+AGG - chr7_1:66221916-66221935 None:intergenic 35.0%
!!! CCTTGTTTTTGTCATTGGAT+TGG + chr7_1:66221913-66221932 Msa1031510:intron 35.0%
!! GGATTGGTCAAACACTAAAA+TGG + chr7_1:66221929-66221948 Msa1031510:intron 35.0%
TTGGTCAAACACTAAAATGG+TGG + chr7_1:66221932-66221951 Msa1031510:intron 35.0%
!! AGATGGCATTGATGAATTTG+GGG - chr7_1:66221970-66221989 None:intergenic 35.0%
TCATCAATGCCATCTTTAGT+TGG + chr7_1:66221975-66221994 Msa1031510:intron 35.0%
TACATATCTGCGGAAAAAGA+TGG - chr7_1:66222084-66222103 None:intergenic 35.0%
CATATAGACATCTGCGTTTA+AGG - chr7_1:66222237-66222256 None:intergenic 35.0%
AGTCATGAGACCATTGATAT+CGG - chr7_1:66222303-66222322 None:intergenic 35.0%
GGGTAAATGTTGACAGTAAA+TGG - chr7_1:66222359-66222378 None:intergenic 35.0%
TGGATTCCATTGTCAGTTAT+TGG - chr7_1:66222449-66222468 None:intergenic 35.0%
CATCAAAGACGTTGGTATAT+TGG - chr7_1:66222469-66222488 None:intergenic 35.0%
GAAATTTGCATCAAAGACGT+TGG - chr7_1:66222477-66222496 None:intergenic 35.0%
GTAGAATCTTATTGCATTGC+CGG - chr7_1:66222600-66222619 None:intergenic 35.0%
AGATAAACTTCGATGAATCC+GGG - chr7_1:66222671-66222690 None:intergenic 35.0%
CAGATAAACTTCGATGAATC+CGG - chr7_1:66222672-66222691 None:intergenic 35.0%
TGGGGAATATTTCGATATGA+TGG + chr7_1:66222749-66222768 Msa1031510:CDS 35.0%
ATTAACTTTGCTTGCACCTT+TGG + chr7_1:66222911-66222930 Msa1031510:CDS 35.0%
AAGCATTTCCATTAGCATCA+AGG - chr7_1:66222976-66222995 None:intergenic 35.0%
GTTGAAATGTTGTCTGTAGT+AGG - chr7_1:66223073-66223092 None:intergenic 35.0%
ATCAAAGAAGATGCTGAAGA+AGG - chr7_1:66223127-66223146 None:intergenic 35.0%
! GAATTTTGCAGATTGAGAGT+TGG + chr7_1:66223292-66223311 Msa1031510:three_prime_UTR 35.0%
! GACGGTGATAGTGATGAAAT+GGG - chr7_1:66220142-66220161 None:intergenic 40.0%
! AGACGGTGATAGTGATGAAA+TGG - chr7_1:66220143-66220162 None:intergenic 40.0%
! TATCACCGTCTTATTCATCG+TGG + chr7_1:66220152-66220171 Msa1031510:five_prime_UTR 40.0%
! ATCACCGTCTTATTCATCGT+GGG + chr7_1:66220153-66220172 Msa1031510:five_prime_UTR 40.0%
GAAACCCACGATGAATAAGA+CGG - chr7_1:66220160-66220179 None:intergenic 40.0%
!!! AGTGGGTTTTGGAGATTAAG+CGG - chr7_1:66220210-66220229 None:intergenic 40.0%
AAGTTAGAGAGAGAAGGGAA+CGG - chr7_1:66220241-66220260 None:intergenic 40.0%
TGTTATTATGTGGAAGGGCT+TGG + chr7_1:66220388-66220407 Msa1031510:CDS 40.0%
! ATCTTCACAACAGTTTCTGG+TGG - chr7_1:66220448-66220467 None:intergenic 40.0%
TGATACCACAATGACTGCTT+TGG + chr7_1:66220513-66220532 Msa1031510:CDS 40.0%
AACGTGCTCAATATTGGGTT+AGG + chr7_1:66220602-66220621 Msa1031510:CDS 40.0%
! TCAAGGAAGCTTTATGTACC+GGG + chr7_1:66221154-66221173 Msa1031510:intron 40.0%
ACTGCAATCAACTCCTAGAA+TGG - chr7_1:66221289-66221308 None:intergenic 40.0%
!! GGAGTTGATTGCAGTTTTAG+GGG + chr7_1:66221294-66221313 Msa1031510:intron 40.0%
!!! CAGTTTTAGGGGAAGAGAAA+TGG + chr7_1:66221305-66221324 Msa1031510:intron 40.0%
! TTTTTGCATGTGCGCGTATA+TGG + chr7_1:66221394-66221413 Msa1031510:intron 40.0%
! TTTTGCATGTGCGCGTATAT+GGG + chr7_1:66221395-66221414 Msa1031510:intron 40.0%
ATTCAATTGTAGCCCTGCTA+TGG + chr7_1:66221665-66221684 Msa1031510:intron 40.0%
!!! TGTCGCCTTGTTTTTGTCAT+TGG + chr7_1:66221908-66221927 Msa1031510:intron 40.0%
CTACTGCTTCCAACTAAAGA+TGG - chr7_1:66221987-66222006 None:intergenic 40.0%
! CTTTTTCCGCAGATATGTAG+CGG + chr7_1:66222085-66222104 Msa1031510:intron 40.0%
! TTTTGAATGTTCTGCAGTGC+AGG - chr7_1:66222167-66222186 None:intergenic 40.0%
CAAAATGCCATCAATGAAGC+TGG + chr7_1:66222182-66222201 Msa1031510:CDS 40.0%
!! TAAGGGTACTGTTGCCTTTA+CGG - chr7_1:66222219-66222238 None:intergenic 40.0%
TTCCATCTGCGGGAATATTT+CGG + chr7_1:66222276-66222295 Msa1031510:CDS 40.0%
ATATTTCGGCCCGATATCAA+TGG + chr7_1:66222290-66222309 Msa1031510:CDS 40.0%
GTCATGAGACCATTGATATC+GGG - chr7_1:66222302-66222321 None:intergenic 40.0%
TACCCCTTCTTAAGTCTCTA+CGG + chr7_1:66222374-66222393 Msa1031510:CDS 40.0%
TTCCCGTAGAGACTTAAGAA+GGG - chr7_1:66222380-66222399 None:intergenic 40.0%
GTTTCTGCTCTCAAATCGAT+CGG + chr7_1:66222506-66222525 Msa1031510:CDS 40.0%
CCTACTTCTCCTACCAAAAT+TGG - chr7_1:66222545-66222564 None:intergenic 40.0%
!! CCAATTTTGGTAGGAGAAGT+AGG + chr7_1:66222542-66222561 Msa1031510:CDS 40.0%
! TTTTGGTAGGAGAAGTAGGA+TGG + chr7_1:66222546-66222565 Msa1031510:CDS 40.0%
TCTTATTGCATTGCCGGAAT+TGG - chr7_1:66222594-66222613 None:intergenic 40.0%
GATAAACTTCGATGAATCCG+GGG - chr7_1:66222670-66222689 None:intergenic 40.0%
! AGGTAACTTTGAGAGACACT+GGG + chr7_1:66222730-66222749 Msa1031510:CDS 40.0%
GAAAGATCCATCGGAAACTT+CGG - chr7_1:66222779-66222798 None:intergenic 40.0%
GAAGTTTCCGATGGATCTTT+CGG + chr7_1:66222778-66222797 Msa1031510:CDS 40.0%
AAGTTTCCGATGGATCTTTC+GGG + chr7_1:66222779-66222798 Msa1031510:CDS 40.0%
AAACTTCTAGTAGGTGCACA+GGG + chr7_1:66222815-66222834 Msa1031510:CDS 40.0%
TGCTGAGATCTTTAGCATCA+GGG - chr7_1:66222877-66222896 None:intergenic 40.0%
TTGCTGAGATCTTTAGCATC+AGG - chr7_1:66222878-66222897 None:intergenic 40.0%
AAGTGCAGTACAATCTCCAA+AGG - chr7_1:66222930-66222949 None:intergenic 40.0%
TTTGGAGATTGTACTGCACT+TGG + chr7_1:66222929-66222948 Msa1031510:CDS 40.0%
TTGGAGATTGTACTGCACTT+GGG + chr7_1:66222930-66222949 Msa1031510:CDS 40.0%
TGCAACAACCTTGATGCTAA+TGG + chr7_1:66222965-66222984 Msa1031510:CDS 40.0%
! CGTCAAGCATGCAATTTTGA+AGG + chr7_1:66223037-66223056 Msa1031510:CDS 40.0%
ATGCTGAAGAAGGAACTATC+TGG - chr7_1:66223117-66223136 None:intergenic 40.0%
AACAACAAAGCAGCAAACGA+GGG - chr7_1:66223151-66223170 None:intergenic 40.0%
AAACAACAAAGCAGCAAACG+AGG - chr7_1:66223152-66223171 None:intergenic 40.0%
!!! CGGTTTTGGAAAGTGGGTTT+TGG - chr7_1:66220221-66220240 None:intergenic 45.0%
!!! AGGGAACGGTTTTGGAAAGT+GGG - chr7_1:66220227-66220246 None:intergenic 45.0%
!!! AAGGGAACGGTTTTGGAAAG+TGG - chr7_1:66220228-66220247 None:intergenic 45.0%
!!! GCGAGTTTTGGGATCAAAGA+AGG + chr7_1:66221077-66221096 Msa1031510:intron 45.0%
! CTAATTCTGAGGGAATCACC+CGG - chr7_1:66221175-66221194 None:intergenic 45.0%
!!! AGTAACTTTTTTCGGTGCCG+AGG + chr7_1:66221233-66221252 Msa1031510:intron 45.0%
AAATCGGTCGAAATTCGGCA+AGG - chr7_1:66222038-66222057 None:intergenic 45.0%
AATGTTCTGCAGTGCAGGAA+AGG - chr7_1:66222162-66222181 None:intergenic 45.0%
ACCAAGACCAGCTTCATTGA+TGG - chr7_1:66222192-66222211 None:intergenic 45.0%
AGATGTCTATATGTCTCCGC+AGG + chr7_1:66222244-66222263 Msa1031510:CDS 45.0%
GATGGAACTTGATTGTCCTG+CGG - chr7_1:66222263-66222282 None:intergenic 45.0%
GGACAATCAAGTTCCATCTG+CGG + chr7_1:66222265-66222284 Msa1031510:CDS 45.0%
GACAATCAAGTTCCATCTGC+GGG + chr7_1:66222266-66222285 Msa1031510:CDS 45.0%
CAATGGTCTCATGACTCAGA+TGG + chr7_1:66222307-66222326 Msa1031510:CDS 45.0%
ACCCCTTCTTAAGTCTCTAC+GGG + chr7_1:66222375-66222394 Msa1031510:CDS 45.0%
TCCCGTAGAGACTTAAGAAG+GGG - chr7_1:66222379-66222398 None:intergenic 45.0%
GTTCCCGTAGAGACTTAAGA+AGG - chr7_1:66222381-66222400 None:intergenic 45.0%
CGAAGAAGGCGAAGTTGAAT+GGG - chr7_1:66222412-66222431 None:intergenic 45.0%
TCGAAGAAGGCGAAGTTGAA+TGG - chr7_1:66222413-66222432 None:intergenic 45.0%
TTCAACTTCGCCTTCTTCGA+CGG + chr7_1:66222413-66222432 Msa1031510:CDS 45.0%
TTTATCTACCCCGTCGAAGA+AGG - chr7_1:66222426-66222445 None:intergenic 45.0%
! GCTCTCAAATCGATCGGTTT+CGG + chr7_1:66222512-66222531 Msa1031510:CDS 45.0%
GCATTCTTGTCACCTTCTGT+TGG - chr7_1:66222572-66222591 None:intergenic 45.0%
GGTGACAAGAATGCCAATTC+CGG + chr7_1:66222578-66222597 Msa1031510:CDS 45.0%
TCTGTTTGGACTCATCGATG+AGG + chr7_1:66222688-66222707 Msa1031510:CDS 45.0%
CAGTGTCTCTCAAAGTTACC+TGG - chr7_1:66222731-66222750 None:intergenic 45.0%
! CAGGTAACTTTGAGAGACAC+TGG + chr7_1:66222729-66222748 Msa1031510:CDS 45.0%
! GGTAACTTTGAGAGACACTG+GGG + chr7_1:66222731-66222750 Msa1031510:CDS 45.0%
AGTTGACCCGAAAGATCCAT+CGG - chr7_1:66222788-66222807 None:intergenic 45.0%
CAAACTTCTAGTAGGTGCAC+AGG + chr7_1:66222814-66222833 Msa1031510:CDS 45.0%
GGGTTAAACATGCACCACAT+TGG - chr7_1:66222857-66222876 None:intergenic 45.0%
GATTGTACTGCACTTGGGTA+TGG + chr7_1:66222935-66222954 Msa1031510:CDS 45.0%
!!! TGATATAATTTTTTTATTTA+AGG + chr7_1:66223222-66223241 Msa1031510:three_prime_UTR 5.0%
AACGGAGAGGCTAAGGAGTA+AGG - chr7_1:66220010-66220029 None:intergenic 50.0%
AGAGAGAAACGGAGAGGCTA+AGG - chr7_1:66220017-66220036 None:intergenic 50.0%
TCTGTCAGAGAGAAACGGAG+AGG - chr7_1:66220023-66220042 None:intergenic 50.0%
! GAGAGAGAAGGGAACGGTTT+TGG - chr7_1:66220235-66220254 None:intergenic 50.0%
ACCACAATGACTGCTTTGGC+TGG + chr7_1:66220517-66220536 Msa1031510:CDS 50.0%
ACCAGCCAAAGCAGTCATTG+TGG - chr7_1:66220521-66220540 None:intergenic 50.0%
ATGACTGCTTTGGCTGGTAG+TGG + chr7_1:66220523-66220542 Msa1031510:CDS 50.0%
TGTCAAAAGCGGCCATAGCA+GGG - chr7_1:66221680-66221699 None:intergenic 50.0%
TTGTCAAAAGCGGCCATAGC+AGG - chr7_1:66221681-66221700 None:intergenic 50.0%
TTCCGCAGATATGTAGCGGT+TGG + chr7_1:66222089-66222108 Msa1031510:intron 50.0%
TCCGCAGATATGTAGCGGTT+GGG + chr7_1:66222090-66222109 Msa1031510:intron 50.0%
TCCCAACCGCTACATATCTG+CGG - chr7_1:66222094-66222113 None:intergenic 50.0%
! GCCATCAATGAAGCTGGTCT+TGG + chr7_1:66222188-66222207 Msa1031510:CDS 50.0%
! TGGTCTTGGTGACACCGTAA+AGG + chr7_1:66222202-66222221 Msa1031510:CDS 50.0%
TCAACTTCGCCTTCTTCGAC+GGG + chr7_1:66222414-66222433 Msa1031510:CDS 50.0%
! TTTCGGCGACACGCCAATTT+TGG + chr7_1:66222529-66222548 Msa1031510:CDS 50.0%
GAAGTAGGATGGCCAACAGA+AGG + chr7_1:66222557-66222576 Msa1031510:CDS 50.0%
TGTTCGCAGCGAGTCTAGAA+AGG - chr7_1:66222631-66222650 None:intergenic 50.0%
TCTAGACTCGCTGCGAACAA+AGG + chr7_1:66222632-66222651 Msa1031510:CDS 50.0%
AAACTTCGATGAATCCGGGG+CGG - chr7_1:66222667-66222686 None:intergenic 50.0%
TGGACAACCGAAGTTTCCGA+TGG + chr7_1:66222769-66222788 Msa1031510:CDS 50.0%
GTACTACCAGACGCAGAATC+AGG + chr7_1:66223012-66223031 Msa1031510:CDS 50.0%
!! TGGAAGGGCTTGGTGTGAAC+TGG + chr7_1:66220398-66220417 Msa1031510:CDS 55.0%
!! GGAAGGGCTTGGTGTGAACT+GGG + chr7_1:66220399-66220418 Msa1031510:CDS 55.0%
!! GAAGGGCTTGGTGTGAACTG+GGG + chr7_1:66220400-66220419 Msa1031510:CDS 55.0%
GGCCGAAATATTCCCGCAGA+TGG - chr7_1:66222281-66222300 None:intergenic 55.0%
CAACTTCGCCTTCTTCGACG+GGG + chr7_1:66222415-66222434 Msa1031510:CDS 55.0%
!! GGCGACACGCCAATTTTGGT+AGG + chr7_1:66222533-66222552 Msa1031510:CDS 55.0%
TCGCAGCGAGTCTAGAAAGG+AGG - chr7_1:66222628-66222647 None:intergenic 55.0%
GACGATCCTGATTCTGCGTC+TGG - chr7_1:66223021-66223040 None:intergenic 55.0%
!! GACGCGAAGAGCATTGCTCC+AGG + chr7_1:66222710-66222729 Msa1031510:CDS 60.0%
AAAGGAACCCCTCTCCGCCC+CGG + chr7_1:66222650-66222669 Msa1031510:CDS 65.0%
GATGAATCCGGGGCGGAGAG+GGG - chr7_1:66222660-66222679 None:intergenic 65.0%
CGATGAATCCGGGGCGGAGA+GGG - chr7_1:66222661-66222680 None:intergenic 65.0%
TCGATGAATCCGGGGCGGAG+AGG - chr7_1:66222662-66222681 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 66219922 66223324 66219922 ID=Msa1031510;Name=Msa1031510
chr7_1 mRNA 66219922 66223324 66219922 ID=Msa1031510-mRNA-1;Parent=Msa1031510;Name=Msa1031510-mRNA-1;_AED=0.05;_eAED=0.05;_QI=409|1|1|1|1|1|2|123|480
chr7_1 exon 66219922 66220668 66219922 ID=Msa1031510-mRNA-1:exon:14827;Parent=Msa1031510-mRNA-1
chr7_1 exon 66222097 66223324 66222097 ID=Msa1031510-mRNA-1:exon:14828;Parent=Msa1031510-mRNA-1
chr7_1 five_prime_UTR 66219922 66220330 66219922 ID=Msa1031510-mRNA-1:five_prime_utr;Parent=Msa1031510-mRNA-1
chr7_1 CDS 66220331 66220668 66220331 ID=Msa1031510-mRNA-1:cds;Parent=Msa1031510-mRNA-1
chr7_1 CDS 66222097 66223201 66222097 ID=Msa1031510-mRNA-1:cds;Parent=Msa1031510-mRNA-1
chr7_1 three_prime_UTR 66223202 66223324 66223202 ID=Msa1031510-mRNA-1:three_prime_utr;Parent=Msa1031510-mRNA-1
Gene Sequence

>Msa1031510

ATGGAAAAAAATTGGATTTTTACAATGTTGTTGATGTTAGGATTATTGGGTTGTTTTTGTTATTATGTGGAAGGGCTTGGTGTGAACTGGGGTACACAAGCAACACACAAGTTACCACCAGAAACTGTTGTGAAGATGTTGAAGGATAATGGGATTCAAAAAGTTAAGCTTTTTGATGCAGATGATACCACAATGACTGCTTTGGCTGGTAGTGGAATTGAAGTTATGGTTGCTATTCCTAATAATCAACTTGCTGAAATGAATGATTATAAACGTGCTCAATATTGGGTTAGGAAGAATGTTACTCGTTATAACTTTGATGGCGGTGTTAAGATCAAATATGTAGCGGTTGGGAATGAGCCATTTTTGAAAGCTTACAATAATTCATTCTTGAACATCACCTTTCCTGCACTGCAGAACATTCAAAATGCCATCAATGAAGCTGGTCTTGGTGACACCGTAAAGGCAACAGTACCCTTAAACGCAGATGTCTATATGTCTCCGCAGGACAATCAAGTTCCATCTGCGGGAATATTTCGGCCCGATATCAATGGTCTCATGACTCAGATGGTACAATTTCTCAACAAGAACGGAGCACCATTTACTGTCAACATTTACCCCTTCTTAAGTCTCTACGGGAACGACGATTTCCCATTCAACTTCGCCTTCTTCGACGGGGTAGATAAACCAATAACTGACAATGGAATCCAATATACCAACGTCTTTGATGCAAATTTCGATACATTGGTTTCTGCTCTCAAATCGATCGGTTTCGGCGACACGCCAATTTTGGTAGGAGAAGTAGGATGGCCAACAGAAGGTGACAAGAATGCCAATTCCGGCAATGCAATAAGATTCTACAATGGCCTCCTTTCTAGACTCGCTGCGAACAAAGGAACCCCTCTCCGCCCCGGATTCATCGAAGTTTATCTGTTTGGACTCATCGATGAGGACGCGAAGAGCATTGCTCCAGGTAACTTTGAGAGACACTGGGGAATATTTCGATATGATGGACAACCGAAGTTTCCGATGGATCTTTCGGGTCAACTTCAAAACAAACTTCTAGTAGGTGCACAGGGTGTGAAATATCTTGATCCAATGTGGTGCATGTTTAACCCTGATGCTAAAGATCTCAGCAAACTTGCTGATAACATTAACTTTGCTTGCACCTTTGGAGATTGTACTGCACTTGGGTATGGATCTTCTTGCAACAACCTTGATGCTAATGGAAATGCTTCTTATGCATTCAACATGTACTACCAGACGCAGAATCAGGATCGTCAAGCATGCAATTTTGAAGGTTTAGCTAAGCCTACTACAGACAACATTTCAACACCTACTTGCAATTTTATCATCCAGATAGTTCCTTCTTCAGCATCTTCTTTGATGCCCTCGTTTGCTGCTTTGTTGTTTATTGCTTTTTCTGTGATTCTATTGGCTTAG
Protein sequence

>Msa1031510

MEKNWIFTMLLMLGLLGCFCYYVEGLGVNWGTQATHKLPPETVVKMLKDNGIQKVKLFDADDTTMTALAGSGIEVMVAIPNNQLAEMNDYKRAQYWVRKNVTRYNFDGGVKIKYVAVGNEPFLKAYNNSFLNITFPALQNIQNAINEAGLGDTVKATVPLNADVYMSPQDNQVPSAGIFRPDINGLMTQMVQFLNKNGAPFTVNIYPFLSLYGNDDFPFNFAFFDGVDKPITDNGIQYTNVFDANFDTLVSALKSIGFGDTPILVGEVGWPTEGDKNANSGNAIRFYNGLLSRLAANKGTPLRPGFIEVYLFGLIDEDAKSIAPGNFERHWGIFRYDGQPKFPMDLSGQLQNKLLVGAQGVKYLDPMWCMFNPDAKDLSKLADNINFACTFGDCTALGYGSSCNNLDANGNASYAFNMYYQTQNQDRQACNFEGLAKPTTDNISTPTCNFIIQIVPSSASSLMPSFAALLFIAFSVILLA*