AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1031520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031520 MtrunA17_Chr7g0253101 95.167 269 5 2 1 269 1 261 0.0 513
Msa1031520 MtrunA17_Chr6g0461591 61.372 277 77 10 1 264 1 260 1.50e-103 301
Msa1031520 MtrunA17_Chr3g0140331 39.200 250 95 9 15 260 121 317 7.75e-35 128
Msa1031520 MtrunA17_Chr1g0199671 76.471 68 16 0 193 260 239 306 5.90e-34 125
Msa1031520 MtrunA17_Chr8g0340401 37.555 229 77 7 45 260 115 290 1.81e-33 124
Msa1031520 MtrunA17_Chr4g0035891 80.000 65 13 0 196 260 211 275 3.28e-33 122
Msa1031520 MtrunA17_Chr2g0326771 61.702 94 35 1 172 264 191 284 3.49e-33 123
Msa1031520 MtrunA17_Chr1g0146231 36.364 264 113 9 11 260 76 298 6.83e-33 123
Msa1031520 MtrunA17_Chr8g0372291 81.538 65 12 0 196 260 218 282 1.84e-32 120
Msa1031520 MtrunA17_Chr1g0169431 74.648 71 18 0 190 260 166 236 6.81e-32 118
Msa1031520 MtrunA17_Chr1g0213161 75.758 66 16 0 196 261 152 217 6.82e-32 117
Msa1031520 MtrunA17_Chr5g0404981 41.048 229 95 8 45 260 163 364 1.08e-31 120
Msa1031520 MtrunA17_Chr3g0145121 76.923 65 15 0 196 260 215 279 3.20e-31 117
Msa1031520 MtrunA17_Chr4g0028961 71.831 71 20 0 193 263 229 299 6.60e-31 117
Msa1031520 MtrunA17_Chr5g0395391 73.846 65 17 0 196 260 270 334 3.07e-29 113
Msa1031520 MtrunA17_Chr5g0395391 71.875 64 18 0 196 259 168 231 1.36e-27 108
Msa1031520 MtrunA17_Chr7g0232671 58.065 62 26 0 196 257 187 248 5.49e-20 86.7
Msa1031520 MtrunA17_Chr7g0232701 56.923 65 28 0 193 257 70 134 7.28e-20 83.2
Msa1031520 MtrunA17_Chr7g0232681 40.000 105 63 0 153 257 89 193 3.12e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1031520 AT4G32890.1 42.793 222 81 6 45 260 80 261 2.11e-43 150
Msa1031520 AT2G45050.1 39.167 240 93 8 21 260 57 243 4.46e-39 137
Msa1031520 AT5G25830.1 38.655 238 88 8 45 260 82 283 2.83e-36 132
Msa1031520 AT3G60530.1 39.631 217 59 8 45 260 77 222 1.20e-35 128
Msa1031520 AT5G66320.2 40.891 247 109 9 28 260 90 313 2.34e-35 129
Msa1031520 AT5G66320.1 40.891 247 109 9 28 260 90 313 2.34e-35 129
Msa1031520 AT3G24050.1 75.000 68 17 0 193 260 191 258 4.99e-33 122
Msa1031520 AT4G36240.1 37.838 222 75 6 45 265 74 233 1.36e-32 120
Msa1031520 AT3G51080.1 81.538 65 12 0 196 260 221 285 5.23e-32 120
Msa1031520 AT1G08010.2 72.308 65 18 0 196 260 220 284 4.69e-31 117
Msa1031520 AT1G08010.1 72.308 65 18 0 196 260 220 284 4.69e-31 117
Msa1031520 AT1G08010.3 72.308 65 18 0 196 260 220 284 4.69e-31 117
Msa1031520 AT1G08000.2 73.846 65 17 0 196 260 218 282 9.11e-30 114
Msa1031520 AT1G08000.1 73.846 65 17 0 196 260 218 282 9.11e-30 114
Msa1031520 AT4G34680.1 72.308 65 18 0 196 260 180 244 1.03e-29 113
Msa1031520 AT4G34680.2 72.308 65 18 0 196 260 180 244 1.03e-29 113
Msa1031520 AT2G28340.1 67.188 64 21 0 198 261 193 256 2.78e-26 104
Msa1031520 AT3G45170.1 54.878 82 37 0 185 266 104 185 3.23e-26 102

Find 69 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTAAAACAAGCTTAT+TGG 0.124697 7_1:-66234713 Msa1031520:CDS
TTTAGGTGAAATTAACTATT+TGG 0.133712 7_1:-66234258 Msa1031520:three_prime_UTR
CACTAGAAACTTGTACATTT+TGG 0.144962 7_1:+66235436 None:intergenic
AACTTAGAGAGCACCAATTT+TGG 0.237029 7_1:-66235561 Msa1031520:five_prime_UTR
GGAAACATGGGATAGTATTT+AGG 0.238628 7_1:-66234237 Msa1031520:three_prime_UTR
CATGCATTGCATAGTGTCTT+TGG 0.243410 7_1:+66234480 None:intergenic
TGAAAAGGAAATTGTGAAAA+AGG 0.260278 7_1:-66234625 Msa1031520:CDS
CATAGTGTCTTTGGGCCTAA+TGG 0.278012 7_1:+66234489 None:intergenic
ACACTACTCACTAGGATTAA+TGG 0.294145 7_1:+66234329 None:intergenic
GTTGTTAGTGTTATGATTAT+TGG 0.299540 7_1:-66234211 Msa1031520:three_prime_UTR
ATGGGCCTGCCCTCCATTGT+GGG 0.318681 7_1:+66234508 None:intergenic
CAATGGAGGGCAGGCCCATT+AGG 0.322478 7_1:-66234504 Msa1031520:CDS
GTGTTGGATAAGATGCTTAA+AGG 0.325601 7_1:+66234774 None:intergenic
AAGATGCTTAAAGGGTCTTT+TGG 0.328925 7_1:+66234783 None:intergenic
GGTTTCTTCACTGTGTTTGA+AGG 0.329936 7_1:+66234888 None:intergenic
ATAGTGTCTTTGGGCCTAAT+GGG 0.339035 7_1:+66234490 None:intergenic
CACACCAAGAGAGGGGAAAA+TGG 0.339492 7_1:+66235516 None:intergenic
AGAGAAAAGAAAGCCAAAAT+TGG 0.351490 7_1:+66235548 None:intergenic
GTACACCTCCTTGTTGGAAT+TGG 0.356244 7_1:+66234552 None:intergenic
ATGCATTGCATAGTGTCTTT+GGG 0.368727 7_1:+66234481 None:intergenic
AATGGGCCTGCCCTCCATTG+TGG 0.406749 7_1:+66234507 None:intergenic
ATCCTAGTGAGTAGTGTTAG+TGG 0.416497 7_1:-66234323 Msa1031520:exon
CAATGTGTACACCTCCTTGT+TGG 0.437336 7_1:+66234546 None:intergenic
TGTTGGATAAGATGCTTAAA+GGG 0.444585 7_1:+66234775 None:intergenic
AAATTCACACAAGAAAGTTA+TGG 0.446713 7_1:-66234376 Msa1031520:CDS
GCTTTGCCTGGAACTGCAAA+AGG 0.456279 7_1:+66234843 None:intergenic
GTAAAACTACACAACTTTGT+GGG 0.468662 7_1:+66234952 None:intergenic
AGATTATGAAGTGGTTGAAA+AGG 0.475929 7_1:-66234640 Msa1031520:CDS
TAGTGAACTCATGGTACCTA+AGG 0.486078 7_1:-66234682 Msa1031520:CDS
AAATTAACTATTTGGAAACA+TGG 0.500713 7_1:-66234250 Msa1031520:three_prime_UTR
CAGGCAAAGCAAGAAGCAAA+AGG 0.500793 7_1:-66234830 Msa1031520:CDS
ACAAAAGTTGGACTCTTAGC+AGG 0.508744 7_1:+66234414 None:intergenic
GGTAAAACTACACAACTTTG+TGG 0.510522 7_1:+66234951 None:intergenic
AGGACCCCACAATGGAGGGC+AGG 0.512119 7_1:-66234513 Msa1031520:CDS
ATTATGTAGGAAGTGGATGT+TGG 0.517271 7_1:-66235017 Msa1031520:intron
TTGAAAGGTTAGAGCAAGAA+GGG 0.531774 7_1:+66235388 None:intergenic
CTTGAAAGGTTAGAGCAAGA+AGG 0.532182 7_1:+66235387 None:intergenic
TTATGAAAGTGAAATGCATA+TGG 0.537832 7_1:+66234090 None:intergenic
TAGTGAGTGCAAACAAGAGA+AGG 0.541293 7_1:-66235418 Msa1031520:CDS
AATTTGAACACACCAAGAGA+GGG 0.554374 7_1:+66235508 None:intergenic
GTTAGCTGATAGTGAACTCA+TGG 0.566356 7_1:-66234691 Msa1031520:CDS
TGGGCCTGCCCTCCATTGTG+GGG 0.567754 7_1:+66234509 None:intergenic
GGTCATCAAGGCTACTTGAA+AGG 0.569542 7_1:+66235373 None:intergenic
GAGCAGAAAAGAGGTCATCA+AGG 0.570986 7_1:+66235361 None:intergenic
CAAGAGAGGGGAAAATGGTA+AGG 0.576200 7_1:+66235521 None:intergenic
TGTGGTGTGAGGTACAAATC+TGG 0.576464 7_1:-66234459 Msa1031520:CDS
GTAGGAAGTGGATGTTGGAA+TGG 0.583317 7_1:-66235012 Msa1031520:intron
AATTAACTATTTGGAAACAT+GGG 0.586539 7_1:-66234249 Msa1031520:three_prime_UTR
TTTGAGGTTGATGTTAACAA+TGG 0.593552 7_1:-66234582 Msa1031520:CDS
ACAAAGGACCCCACAATGGA+GGG 0.595325 7_1:-66234517 Msa1031520:CDS
CTCTTAGCAGGTCTATACTC+AGG 0.605156 7_1:+66234426 None:intergenic
CACAAAGGACCCCACAATGG+AGG 0.605327 7_1:-66234518 Msa1031520:CDS
AAATTTGAACACACCAAGAG+AGG 0.609940 7_1:+66235507 None:intergenic
ACAAGAAAGTTATGGAGATG+AGG 0.611218 7_1:-66234368 Msa1031520:CDS
GGTAACAAAAGATTATGAAG+TGG 0.619135 7_1:-66234649 Msa1031520:CDS
AGTGAACTCATGGTACCTAA+GGG 0.625585 7_1:-66234681 Msa1031520:CDS
AACCACTAACACTACTCACT+AGG 0.631055 7_1:+66234321 None:intergenic
AAGTGGATGTTGGAATGGAA+TGG 0.634243 7_1:-66235007 Msa1031520:CDS
TGCATATGGTATGTAACATG+AGG 0.635500 7_1:+66234104 None:intergenic
GTCAAAATCCAATTCCAACA+AGG 0.638845 7_1:-66234560 Msa1031520:CDS
GTACACATTGTTTGTCACAA+AGG 0.655851 7_1:-66234533 Msa1031520:CDS
AAACACAGTGAAGAAACCAA+AGG 0.660234 7_1:-66234883 Msa1031520:CDS
ATATGGTATGTAACATGAGG+AGG 0.661521 7_1:+66234107 None:intergenic
GTGTGAGGTACAAATCTGGT+AGG 0.664094 7_1:-66234455 Msa1031520:CDS
ATTTGAACACACCAAGAGAG+GGG 0.671203 7_1:+66235509 None:intergenic
TGTCACAAAGGACCCCACAA+TGG 0.681006 7_1:-66234521 Msa1031520:CDS
AAAATCCAATTCCAACAAGG+AGG 0.704851 7_1:-66234557 Msa1031520:CDS
TATGCAATGCATGTGGTGTG+AGG 0.734829 7_1:-66234470 Msa1031520:CDS
AAGACACTATGCAATGCATG+TGG 0.751831 7_1:-66234477 Msa1031520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAGAAACATAATATTAA+AGG + chr7_1:66234529-66234548 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_1:66235373-66235392 Msa1031520:CDS 10.0%
!! AATTAGAAACATAATATTAA+AGG + chr7_1:66234529-66234548 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_1:66235373-66235392 Msa1031520:CDS 10.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_1:66234533-66234552 Msa1031520:CDS 15.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_1:66234533-66234552 Msa1031520:CDS 15.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_1:66234577-66234596 Msa1031520:CDS 20.0%
!! TTTAGGTGAAATTAACTATT+TGG - chr7_1:66235390-66235409 Msa1031520:CDS 20.0%
!! AAATTAACTATTTGGAAACA+TGG - chr7_1:66235398-66235417 Msa1031520:CDS 20.0%
!! AATTAACTATTTGGAAACAT+GGG - chr7_1:66235399-66235418 Msa1031520:CDS 20.0%
!!! TATCCTGTTTTTTTTTTTGA+AGG - chr7_1:66235487-66235506 Msa1031520:five_prime_UTR 20.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_1:66234577-66234596 Msa1031520:CDS 20.0%
!! TTTAGGTGAAATTAACTATT+TGG - chr7_1:66235390-66235409 Msa1031520:CDS 20.0%
!! AAATTAACTATTTGGAAACA+TGG - chr7_1:66235398-66235417 Msa1031520:CDS 20.0%
!! AATTAACTATTTGGAAACAT+GGG - chr7_1:66235399-66235418 Msa1031520:CDS 20.0%
!!! TATCCTGTTTTTTTTTTTGA+AGG - chr7_1:66235487-66235506 Msa1031520:five_prime_UTR 20.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_1:66234176-66234195 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_1:66234177-66234196 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_1:66234182-66234201 Msa1031520:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_1:66234442-66234461 None:intergenic 25.0%
!! TTTTGACCAAACAATTATGT+AGG - chr7_1:66234618-66234637 Msa1031520:CDS 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_1:66235023-66235042 Msa1031520:intron 25.0%
! AAATTCACACAAGAAAGTTA+TGG - chr7_1:66235272-66235291 Msa1031520:intron 25.0%
! GTTGTTAGTGTTATGATTAT+TGG - chr7_1:66235437-66235456 Msa1031520:CDS 25.0%
! CTTCCTTCAAAAAAAAAAAC+AGG + chr7_1:66235493-66235512 None:intergenic 25.0%
! TTATGAAAGTGAAATGCATA+TGG + chr7_1:66235561-66235580 None:intergenic 25.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_1:66234176-66234195 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_1:66234177-66234196 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_1:66234182-66234201 Msa1031520:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_1:66234442-66234461 None:intergenic 25.0%
!! TTTTGACCAAACAATTATGT+AGG - chr7_1:66234618-66234637 Msa1031520:CDS 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_1:66235023-66235042 Msa1031520:intron 25.0%
! AAATTCACACAAGAAAGTTA+TGG - chr7_1:66235272-66235291 Msa1031520:intron 25.0%
! GTTGTTAGTGTTATGATTAT+TGG - chr7_1:66235437-66235456 Msa1031520:CDS 25.0%
! CTTCCTTCAAAAAAAAAAAC+AGG + chr7_1:66235493-66235512 None:intergenic 25.0%
! TTATGAAAGTGAAATGCATA+TGG + chr7_1:66235561-66235580 None:intergenic 25.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_1:66234103-66234122 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_1:66234175-66234194 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_1:66234215-66234234 None:intergenic 30.0%
GTAAAACTACACAACTTTGT+GGG + chr7_1:66234699-66234718 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_1:66234721-66234740 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_1:66234722-66234741 None:intergenic 30.0%
TGTTGGATAAGATGCTTAAA+GGG + chr7_1:66234876-66234895 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_1:66234906-66234925 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_1:66234937-66234956 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_1:66234935-66234954 Msa1031520:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_1:66234999-66235018 Msa1031520:CDS 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_1:66235008-66235027 Msa1031520:CDS 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_1:66235028-66235047 Msa1031520:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_1:66235041-66235060 Msa1031520:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_1:66235042-66235061 Msa1031520:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_1:66235066-66235085 Msa1031520:intron 30.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_1:66234103-66234122 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_1:66234175-66234194 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_1:66234215-66234234 None:intergenic 30.0%
GTAAAACTACACAACTTTGT+GGG + chr7_1:66234699-66234718 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_1:66234721-66234740 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_1:66234722-66234741 None:intergenic 30.0%
TGTTGGATAAGATGCTTAAA+GGG + chr7_1:66234876-66234895 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_1:66234906-66234925 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_1:66234937-66234956 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_1:66234935-66234954 Msa1031520:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_1:66234999-66235018 Msa1031520:CDS 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_1:66235008-66235027 Msa1031520:CDS 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_1:66235028-66235047 Msa1031520:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_1:66235041-66235060 Msa1031520:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_1:66235042-66235061 Msa1031520:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_1:66235066-66235085 Msa1031520:intron 30.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_1:66234087-66234106 Msa1031520:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_1:66234143-66234162 None:intergenic 35.0%
AAATTTGAACACACCAAGAG+AGG + chr7_1:66234144-66234163 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_1:66234174-66234193 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_1:66234263-66234282 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_1:66234418-66234437 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_1:66234431-66234450 None:intergenic 35.0%
CCACTTCCTACATAATTGTT+TGG + chr7_1:66234627-66234646 None:intergenic 35.0%
CCAAACAATTATGTAGGAAG+TGG - chr7_1:66234624-66234643 Msa1031520:CDS 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_1:66234631-66234650 Msa1031520:CDS 35.0%
GGTAAAACTACACAACTTTG+TGG + chr7_1:66234700-66234719 None:intergenic 35.0%
AAACACAGTGAAGAAACCAA+AGG - chr7_1:66234765-66234784 Msa1031520:CDS 35.0%
! GACTCATTTTGTTCTTCCTT+TGG + chr7_1:66234784-66234803 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_1:66234868-66234887 None:intergenic 35.0%
GTGTTGGATAAGATGCTTAA+AGG + chr7_1:66234877-66234896 None:intergenic 35.0%
!! ATTTTGAGGGTTTAAGGTGT+TGG + chr7_1:66234893-66234912 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_1:66234899-66234918 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_1:66234907-66234926 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_1:66234933-66234952 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_1:66234934-66234953 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_1:66235043-66235062 Msa1031520:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_1:66235088-66235107 Msa1031520:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_1:66235091-66235110 Msa1031520:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_1:66235115-66235134 Msa1031520:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_1:66235170-66235189 None:intergenic 35.0%
TGCAAAAAGCTCACAAAAGT+TGG + chr7_1:66235249-66235268 None:intergenic 35.0%
ACAAGAAAGTTATGGAGATG+AGG - chr7_1:66235280-66235299 Msa1031520:intron 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_1:66235322-66235341 None:intergenic 35.0%
GGAAACATGGGATAGTATTT+AGG - chr7_1:66235411-66235430 Msa1031520:CDS 35.0%
ATATGGTATGTAACATGAGG+AGG + chr7_1:66235544-66235563 None:intergenic 35.0%
TGCATATGGTATGTAACATG+AGG + chr7_1:66235547-66235566 None:intergenic 35.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_1:66234087-66234106 Msa1031520:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_1:66234143-66234162 None:intergenic 35.0%
AAATTTGAACACACCAAGAG+AGG + chr7_1:66234144-66234163 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_1:66234174-66234193 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_1:66234263-66234282 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_1:66234418-66234437 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_1:66234431-66234450 None:intergenic 35.0%
CCACTTCCTACATAATTGTT+TGG + chr7_1:66234627-66234646 None:intergenic 35.0%
CCAAACAATTATGTAGGAAG+TGG - chr7_1:66234624-66234643 Msa1031520:CDS 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_1:66234631-66234650 Msa1031520:CDS 35.0%
GGTAAAACTACACAACTTTG+TGG + chr7_1:66234700-66234719 None:intergenic 35.0%
AAACACAGTGAAGAAACCAA+AGG - chr7_1:66234765-66234784 Msa1031520:CDS 35.0%
! GACTCATTTTGTTCTTCCTT+TGG + chr7_1:66234784-66234803 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_1:66234868-66234887 None:intergenic 35.0%
GTGTTGGATAAGATGCTTAA+AGG + chr7_1:66234877-66234896 None:intergenic 35.0%
!! ATTTTGAGGGTTTAAGGTGT+TGG + chr7_1:66234893-66234912 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_1:66234899-66234918 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_1:66234907-66234926 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_1:66234933-66234952 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_1:66234934-66234953 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_1:66235043-66235062 Msa1031520:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_1:66235088-66235107 Msa1031520:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_1:66235091-66235110 Msa1031520:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_1:66235115-66235134 Msa1031520:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_1:66235170-66235189 None:intergenic 35.0%
TGCAAAAAGCTCACAAAAGT+TGG + chr7_1:66235249-66235268 None:intergenic 35.0%
ACAAGAAAGTTATGGAGATG+AGG - chr7_1:66235280-66235299 Msa1031520:intron 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_1:66235322-66235341 None:intergenic 35.0%
GGAAACATGGGATAGTATTT+AGG - chr7_1:66235411-66235430 Msa1031520:CDS 35.0%
ATATGGTATGTAACATGAGG+AGG + chr7_1:66235544-66235563 None:intergenic 35.0%
TGCATATGGTATGTAACATG+AGG + chr7_1:66235547-66235566 None:intergenic 35.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_1:66234142-66234161 None:intergenic 40.0%
GTGTAGAAAAAGTTAAGGGG+GGG + chr7_1:66234172-66234191 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_1:66234173-66234192 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_1:66234194-66234213 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_1:66234230-66234249 Msa1031520:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_1:66234264-66234283 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_1:66234296-66234315 Msa1031520:three_prime_UTR 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_1:66234427-66234446 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_1:66234641-66234660 Msa1031520:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_1:66234763-66234782 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_1:66234859-66234878 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_1:66234957-66234976 Msa1031520:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_1:66234966-66234985 Msa1031520:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_1:66234967-66234986 Msa1031520:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_1:66234985-66235004 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_1:66235050-66235069 Msa1031520:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_1:66235161-66235180 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_1:66235171-66235190 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_1:66235171-66235190 Msa1031520:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_1:66235237-66235256 None:intergenic 40.0%
ATCCTAGTGAGTAGTGTTAG+TGG - chr7_1:66235325-66235344 Msa1031520:intron 40.0%
AACCACTAACACTACTCACT+AGG + chr7_1:66235330-66235349 None:intergenic 40.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_1:66234142-66234161 None:intergenic 40.0%
GTGTAGAAAAAGTTAAGGGG+GGG + chr7_1:66234172-66234191 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_1:66234173-66234192 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_1:66234194-66234213 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_1:66234230-66234249 Msa1031520:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_1:66234264-66234283 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_1:66234296-66234315 Msa1031520:three_prime_UTR 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_1:66234427-66234446 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_1:66234641-66234660 Msa1031520:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_1:66234763-66234782 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_1:66234859-66234878 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_1:66234957-66234976 Msa1031520:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_1:66234966-66234985 Msa1031520:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_1:66234967-66234986 Msa1031520:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_1:66234985-66235004 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_1:66235050-66235069 Msa1031520:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_1:66235161-66235180 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_1:66235171-66235190 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_1:66235171-66235190 Msa1031520:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_1:66235237-66235256 None:intergenic 40.0%
ATCCTAGTGAGTAGTGTTAG+TGG - chr7_1:66235325-66235344 Msa1031520:intron 40.0%
AACCACTAACACTACTCACT+AGG + chr7_1:66235330-66235349 None:intergenic 40.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_1:66234130-66234149 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_1:66234128-66234147 Msa1031520:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_1:66234278-66234297 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_1:66234290-66234309 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_1:66234299-66234318 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_1:66234636-66234655 Msa1031520:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_1:66234820-66234839 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_1:66234818-66234837 Msa1031520:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_1:66234978-66234997 Msa1031520:CDS 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_1:66235099-66235118 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_1:66235105-66235124 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_1:66235162-66235181 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_1:66235178-66235197 Msa1031520:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_1:66235189-66235208 Msa1031520:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_1:66235193-66235212 Msa1031520:intron 45.0%
CTCTTAGCAGGTCTATACTC+AGG + chr7_1:66235225-66235244 None:intergenic 45.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_1:66234130-66234149 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_1:66234128-66234147 Msa1031520:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_1:66234278-66234297 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_1:66234290-66234309 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_1:66234299-66234318 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_1:66234636-66234655 Msa1031520:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_1:66234820-66234839 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_1:66234818-66234837 Msa1031520:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_1:66234978-66234997 Msa1031520:CDS 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_1:66235099-66235118 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_1:66235105-66235124 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_1:66235162-66235181 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_1:66235178-66235197 Msa1031520:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_1:66235189-66235208 Msa1031520:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_1:66235193-66235212 Msa1031520:intron 45.0%
CTCTTAGCAGGTCTATACTC+AGG + chr7_1:66235225-66235244 None:intergenic 45.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_1:66234135-66234154 None:intergenic 50.0%
! GAGTCACCTTTTGCAGTTCC+AGG - chr7_1:66234799-66234818 Msa1031520:CDS 50.0%
GCTTTGCCTGGAACTGCAAA+AGG + chr7_1:66234808-66234827 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_1:66235127-66235146 Msa1031520:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_1:66235131-66235150 Msa1031520:intron 50.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_1:66234135-66234154 None:intergenic 50.0%
! GAGTCACCTTTTGCAGTTCC+AGG - chr7_1:66234799-66234818 Msa1031520:CDS 50.0%
GCTTTGCCTGGAACTGCAAA+AGG + chr7_1:66234808-66234827 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_1:66235127-66235146 Msa1031520:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_1:66235131-66235150 Msa1031520:intron 50.0%
CACAAAGGACCCCACAATGG+AGG - chr7_1:66235130-66235149 Msa1031520:intron 55.0%
CACAAAGGACCCCACAATGG+AGG - chr7_1:66235130-66235149 Msa1031520:intron 55.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_1:66235143-66235162 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_1:66235144-66235163 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_1:66235144-66235163 Msa1031520:intron 60.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_1:66235143-66235162 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_1:66235144-66235163 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_1:66235144-66235163 Msa1031520:intron 60.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_1:66235135-66235154 Msa1031520:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_1:66235142-66235161 None:intergenic 65.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_1:66235135-66235154 Msa1031520:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_1:66235142-66235161 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 66234084 66235586 66234084 ID=Msa1031520;Name=Msa1031520
chr7_1 mRNA 66234084 66235586 66234084 ID=Msa1031520-mRNA-1;Parent=Msa1031520;Name=Msa1031520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=117|1|1|1|1|1|2|248|271
chr7_1 exon 66234084 66235030 66234084 ID=Msa1031520-mRNA-1:exon:14830;Parent=Msa1031520-mRNA-1
chr7_1 exon 66235353 66235586 66235353 ID=Msa1031520-mRNA-1:exon:14829;Parent=Msa1031520-mRNA-1
chr7_1 five_prime_UTR 66235470 66235586 66235470 ID=Msa1031520-mRNA-1:five_prime_utr;Parent=Msa1031520-mRNA-1
chr7_1 CDS 66235353 66235469 66235353 ID=Msa1031520-mRNA-1:cds;Parent=Msa1031520-mRNA-1
chr7_1 CDS 66234332 66235030 66234332 ID=Msa1031520-mRNA-1:cds;Parent=Msa1031520-mRNA-1
chr7_1 three_prime_UTR 66234084 66234331 66234084 ID=Msa1031520-mRNA-1:three_prime_utr;Parent=Msa1031520-mRNA-1
Gene Sequence

>Msa1031520

ATGGATGTGTGCCAAAATGTACAAGTTTCTAGTGAGTGCAAACAAGAGAAGGTTCTTAACCCTTCTTGCTCTAACCTTTCAAGTAGCCTTGATGACCTCTTTTCTGCTCAAAACACGGAAGTGGATGTTGGAATGGAATGGCTTTCAGTGTTTGTTGAAGACTGTTTCTCAAGCCCACAAAGTTGTGTAGTTTTACCCTCTAATGTTCAAAACACAACAAGTACTGTCTCATCAAAACCTTCAAACACAGTGAAGAAACCAAAGGAAGAACAAAATGAGTCACCTTTTGCAGTTCCAGGCAAAGCAAGAAGCAAAAGGAAGAGACTTTCAGCTCCAAGAAGACCAAAAGACCCTTTAAGCATCTTATCCAACACCTTAAACCCTCAAAATGAGTCACTATGTTCTGACCCACCTCTCTTAAAACAAGCTTATTGGTTAGCTGATAGTGAACTCATGGTACCTAAGGGTGAAAAAGAGGTAACAAAAGATTATGAAGTGGTTGAAAAGGAAATTGTGAAAAAGGAAAGGTTTGATTTTGAGGGGTTTGAGGTTGATGTTAACAATGGTCAAAATCCAATTCCAACAAGGAGGTGTACACATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGCCCATTAGGCCCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAATCTGGTAGGTTGTTGCCTGAGTATAGACCTGCTAAGAGTCCAACTTTTGTGAGCTTTTTGCATTCAAATTCACACAAGAAAGTTATGGAGATGAGGATGAATGTTGTATCTTCCATTAATCCTAGTGAGTAG
Protein sequence

>Msa1031520

MDVCQNVQVSSECKQEKVLNPSCSNLSSSLDDLFSAQNTEVDVGMEWLSVFVEDCFSSPQSCVVLPSNVQNTTSTVSSKPSNTVKKPKEEQNESPFAVPGKARSKRKRLSAPRRPKDPLSILSNTLNPQNESLCSDPPLLKQAYWLADSELMVPKGEKEVTKDYEVVEKEIVKKERFDFEGFEVDVNNGQNPIPTRRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSFLHSNSHKKVMEMRMNVVSSINPSE*