Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1038100 | XP_013450565.1 | 98.977 | 684 | 7 | 0 | 1 | 684 | 1 | 684 | 0.0 | 1395 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1038100 | sp|Q86YJ7|AN13B_HUMAN | 34.058 | 276 | 163 | 8 | 68 | 336 | 25 | 288 | 3.47e-34 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1038100 | A0A072U620 | 98.977 | 684 | 7 | 0 | 1 | 684 | 1 | 684 | 0.0 | 1395 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1035060 | Msa1038100 | 0.807542 | 4.803293e-50 | -8.615850e-47 |
| Msa1038100 | Msa1078130 | 0.800856 | 1.184372e-48 | -8.615850e-47 |
| Msa1038100 | Msa1129670 | 0.912589 | 1.638760e-83 | -8.615850e-47 |
| Msa1038100 | Msa1337880 | 0.812575 | 3.956692e-51 | -8.615850e-47 |
| Msa1038100 | Msa1400990 | 0.979899 | 5.423379e-149 | -8.615850e-47 |
| Msa1038100 | Msa1446800 | 0.887490 | 1.362961e-72 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1038100 | MtrunA17_Chr7g0274521 | 98.977 | 684 | 7 | 0 | 1 | 684 | 1 | 684 | 0.0 | 1395 |
| Msa1038100 | MtrunA17_Chr1g0190281 | 71.922 | 666 | 142 | 6 | 27 | 684 | 1 | 629 | 0.0 | 941 |
| Msa1038100 | MtrunA17_Chr1g0169341 | 55.694 | 641 | 245 | 13 | 29 | 646 | 5 | 629 | 0.0 | 669 |
| Msa1038100 | MtrunA17_Chr2g0288551 | 44.745 | 628 | 256 | 9 | 32 | 648 | 26 | 573 | 2.09e-175 | 513 |
| Msa1038100 | MtrunA17_Chr1g0169571 | 48.315 | 356 | 174 | 3 | 29 | 383 | 6 | 352 | 7.39e-109 | 341 |
| Msa1038100 | MtrunA17_Chr1g0169571 | 52.976 | 168 | 74 | 3 | 496 | 660 | 404 | 569 | 1.30e-44 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1038100 | AT3G04470.1 | 74.482 | 627 | 130 | 11 | 27 | 648 | 1 | 602 | 0.0 | 898 |
| Msa1038100 | AT1G04780.1 | 54.038 | 681 | 243 | 16 | 29 | 663 | 7 | 663 | 0.0 | 680 |
| Msa1038100 | AT3G24210.1 | 54.487 | 624 | 235 | 14 | 29 | 644 | 5 | 587 | 0.0 | 624 |
| Msa1038100 | AT1G62050.1 | 51.807 | 332 | 150 | 3 | 33 | 361 | 18 | 342 | 1.50e-116 | 363 |
| Msa1038100 | AT1G62050.1 | 50.323 | 155 | 66 | 1 | 500 | 643 | 444 | 598 | 4.64e-39 | 153 |
| Msa1038100 | AT1G11740.1 | 51.961 | 306 | 140 | 2 | 33 | 338 | 17 | 315 | 1.45e-104 | 332 |
| Msa1038100 | AT1G11740.1 | 50.617 | 162 | 61 | 3 | 500 | 643 | 365 | 525 | 4.67e-40 | 156 |
Find 222 sgRNAs with CRISPR-Local
Find 320 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGTTTGGTCTGATCGT+TGG | 0.521844 | 7_1:-74911532 | None:intergenic |
| AAACATCAAAGAAAACTTCT+AGG | 0.244238 | 7_1:+74912191 | Msa1038100:CDS |
| AAAGAGAAGAAGAGCTGGTT+TGG | 0.397445 | 7_1:+74912115 | Msa1038100:CDS |
| AAATCGATGATCATCGACAT+AGG | 0.590060 | 7_1:-74914151 | None:intergenic |
| AAATGTGCAGGGCTGGAAAG+TGG | 0.638368 | 7_1:-74913541 | None:intergenic |
| AACCTTTGGCTTGGGCTAAA+TGG | 0.249992 | 7_1:+74911315 | Msa1038100:CDS |
| AACTCAAGATTGAATAACCA+TGG | 0.604913 | 7_1:+74910845 | Msa1038100:exon |
| AAGAAGAAGTACCTTAGCTT+AGG | 0.474027 | 7_1:-74910442 | None:intergenic |
| AAGAGAAGACAGAGATGGTT+GGG | 0.468938 | 7_1:+74911792 | Msa1038100:CDS |
| AAGAGGGAAAAGAGAGAGGT+AGG | 0.438577 | 7_1:+74910909 | Msa1038100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGAAATCTGCAAACAGAAA+AGG | - | chr7_1:74910617-74910636 | None:intergenic | 30.0% | |
| GGAAGATCTAGAAAATTGAA+AGG | + | chr7_1:74910776-74910795 | Msa1038100:five_prime_UTR | 30.0% | |
| AACTCAAGATTGAATAACCA+TGG | + | chr7_1:74910845-74910864 | Msa1038100:exon | 30.0% | |
| ATATTTAGTCAAATCCTCCA+TGG | - | chr7_1:74910943-74910962 | None:intergenic | 30.0% | |
| TATTCGATAAGTATCAGAAG+GGG | - | chr7_1:74911450-74911469 | None:intergenic | 30.0% | |
| CATATTCGATAAGTATCAGA+AGG | - | chr7_1:74911452-74911471 | None:intergenic | 30.0% | |
| CTTCTGATACTTATCGAATA+TGG | + | chr7_1:74911450-74911469 | Msa1038100:CDS | 30.0% | |
| AGCTTATTCCTCATTTATCT+TGG | + | chr7_1:74911762-74911781 | Msa1038100:CDS | 30.0% | |
| TTATTCCTCATTTATCTTGG+AGG | + | chr7_1:74911765-74911784 | Msa1038100:CDS | 30.0% | |
| TTATGACATGCTTAATGTGA+TGG | + | chr7_1:74911832-74911851 | Msa1038100:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 74910201 | 74914355 | 74910201 | ID=Msa1038100;Name=Msa1038100 |
| chr7_1 | mRNA | 74910201 | 74914355 | 74910201 | ID=Msa1038100-mRNA-1;Parent=Msa1038100;Name=Msa1038100-mRNA-1;_AED=0.01;_eAED=0.01;_QI=486|1|1|1|0.5|0.33|3|518|684 |
| chr7_1 | exon | 74910201 | 74910452 | 74910201 | ID=Msa1038100-mRNA-1:exon:18951;Parent=Msa1038100-mRNA-1 |
| chr7_1 | exon | 74910630 | 74912534 | 74910630 | ID=Msa1038100-mRNA-1:exon:18952;Parent=Msa1038100-mRNA-1 |
| chr7_1 | exon | 74913454 | 74914355 | 74913454 | ID=Msa1038100-mRNA-1:exon:18953;Parent=Msa1038100-mRNA-1 |
| chr7_1 | five_prime_UTR | 74910201 | 74910452 | 74910201 | ID=Msa1038100-mRNA-1:five_prime_utr;Parent=Msa1038100-mRNA-1 |
| chr7_1 | five_prime_UTR | 74910630 | 74910863 | 74910630 | ID=Msa1038100-mRNA-1:five_prime_utr;Parent=Msa1038100-mRNA-1 |
| chr7_1 | CDS | 74910864 | 74912534 | 74910864 | ID=Msa1038100-mRNA-1:cds;Parent=Msa1038100-mRNA-1 |
| chr7_1 | CDS | 74913454 | 74913837 | 74913454 | ID=Msa1038100-mRNA-1:cds;Parent=Msa1038100-mRNA-1 |
| chr7_1 | three_prime_UTR | 74913838 | 74914355 | 74913838 | ID=Msa1038100-mRNA-1:three_prime_utr;Parent=Msa1038100-mRNA-1 |
| Gene Sequence |
>Msa1038100 ATGGAAGCAAATGGAGTTGTACATGATAACTGTGTAGGAGCTGCGAAGAGGGAAAAGAGAGAGGTAGGAGATAAATCCATGGAGGATTTGACTAAATATACACACAGTCCTGCTCACTTGGCAGTTGCAAGACGTGACCATGCTGCTCTTAGGCGCATTGTTACTGCCCTGCCTCAGCTTGCTAAGGCTGGTGAGGTTAAAAATGAAGCTGAATCTCTTGCTGCTGAACTTCGAGCTGATGAGGTATCGTCTGTTATTGATCGTCGTGATGTACCTGCTAGAGAGACGCCACTTCACCTTGCCGTGCGTCTTAGGGATCATGTCTCTGCTGAGATATTGATGGCTGCTGGTGCGGATTGGAGTTTACAGAACGAGCATGGTTGGAGTGCTCTCCAAGAGGCGGTTTGCACTAGAGAGGAAGCTATTGCTATGATTATTGCACGCCACTATCAACCTTTGGCTTGGGCTAAATGGTGTCGCAGACTTCCCCGTATTGTTGCTTCAGCAACTCGTATTCGTGATTTTTATATGGAGATAACTTTTCATTTTGAAAGCTCTGTAATTCCGTTTATTGGACGCATAGCCCCTTCTGATACTTATCGAATATGGAAGCGTGGTTCTAATCTCCGTGCTGATATGACCCTAGCTGGTTTTGATGGTCTGCGCATCCAACGATCAGACCAAACATTTTTGTTCCTTGGAGAGGGTTATACTTCAGAGGACGGTAATTTAACACTGCCACATGGTTCTTTGCTTGCTCTTTCTCATAAGGAGAAGGAAGTTACAAATGCCTTGGAAGGGGCTGGTACACAACCAACAGAAGCTGAAGTTGCCCATGAAGTCTCCTTGATGTCTCAGACAAATATGTACAGACCAGGTATTGATGTTACTCAGGCTGAGCTTATTCCTCATTTATCTTGGAGGCGTCAAGAGAAGACAGAGATGGTTGGGAATTGGAAAGCAAAAGTTTATGACATGCTTAATGTGATGGTGAGTGTGAAATCAAGACGGGTTCCAGGTGCTATGACAGATGAAGAGCTTTTTGCTGTGGAGGATGGAGAAAGTATGATAAATGGGGAAAACAATGATGAGTACGATGATGTACTGACTGCCGAGGAAAGAATGCAATTAGATTCTGCACTAAGAATGGGGAACTCTGACGCTGCTTGTGAAGATGAAGAGCATGGGGTTGATGGTCAAGAAAATGGTTCATCAGCTTCCAATGAGAATTTTGAAGCCAATGGTGTGGTTAAAGAGAAGAAGAGCTGGTTTGGTTGGAACAAGAAAAGTTTGAAAAGCAACAGTGATGATCCTCCAGAGGATTTAAAAACATCAAAGAAAACTTCTAGGTTTGGCCCAGAAGGTAGCAACCAGAGATTGGCTGATCAAAAGATAGCATCCGAGTTTCTGAAGGATGATAGTGGAGACACCAAGAAGGGAAAGGATAAAAACATTAAAAAGAAGAAGAAGAAAGGAGCAGTTAGCGAGACTAAGACCGAGAGTGAGTATAAAAAGGGTTTAAGACCTGTATTGTGGTTGACGCCTGACTTCCCATTGAAAACCGACGAACTTCTGCCTCTACTTGACATTTTAGCAAATAAGGTTAAAGCTATCAGAAGACTACGAGAGCTTTTGACAACTAAGCTTCCTCAAGGAACTTTTCCTGTCAAGGTTGCTATCCCCATAGTTCCTACTATCAGGGTGCTTGTCACTTTTACAAAATTTGAGGAGCTTCAGCCAATAGAGGAGTTTTCAACACCACTTTCCAGCCCTGCACATTTTCAGGATGCCAAATCCAAGGAGTCAGAGGGGTCCGCGTCATGGATTTCATGGATGAAAGGAGGCCGCGGGGGGCAGTCCAGCGACTCTGACAGTCACAGATATAAGGATGAAGCTGATCCTTTCAGCATACCTGCAGACTACAAATGGGTGGACGCCAATGAAAAGAAACGACGAATGAAGGCGAAGAAAGCCAAAAGTAAGAAACATAAGAAGCCGCCAGTGGCTAAATCTGGAGATGGAGTGCATCGCAGCACCGAGGACATAGAAGAATAG |
| Protein sequence |
>Msa1038100 MEANGVVHDNCVGAAKREKREVGDKSMEDLTKYTHSPAHLAVARRDHAALRRIVTALPQLAKAGEVKNEAESLAAELRADEVSSVIDRRDVPARETPLHLAVRLRDHVSAEILMAAGADWSLQNEHGWSALQEAVCTREEAIAMIIARHYQPLAWAKWCRRLPRIVASATRIRDFYMEITFHFESSVIPFIGRIAPSDTYRIWKRGSNLRADMTLAGFDGLRIQRSDQTFLFLGEGYTSEDGNLTLPHGSLLALSHKEKEVTNALEGAGTQPTEAEVAHEVSLMSQTNMYRPGIDVTQAELIPHLSWRRQEKTEMVGNWKAKVYDMLNVMVSVKSRRVPGAMTDEELFAVEDGESMINGENNDEYDDVLTAEERMQLDSALRMGNSDAACEDEEHGVDGQENGSSASNENFEANGVVKEKKSWFGWNKKSLKSNSDDPPEDLKTSKKTSRFGPEGSNQRLADQKIASEFLKDDSGDTKKGKDKNIKKKKKKGAVSETKTESEYKKGLRPVLWLTPDFPLKTDELLPLLDILANKVKAIRRLRELLTTKLPQGTFPVKVAIPIVPTIRVLVTFTKFEELQPIEEFSTPLSSPAHFQDAKSKESEGSASWISWMKGGRGGQSSDSDSHRYKDEADPFSIPADYKWVDANEKKRRMKAKKAKSKKHKKPPVAKSGDGVHRSTEDIEE* |
