Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069400 | XP_003624413.1 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.72e-90 | 267 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069400 | G7KVA0 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 1.78e-90 | 267 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0671640 | Msa1069400 | 0.810024 | 1.415629e-50 | -8.615850e-47 |
| Msa0708680 | Msa1069400 | 0.805238 | 1.469750e-49 | -8.615850e-47 |
| Msa0814160 | Msa1069400 | 0.823606 | 1.265261e-53 | -8.615850e-47 |
| Msa0134570 | Msa1069400 | 0.800815 | 1.207286e-48 | -8.615850e-47 |
| Msa0366510 | Msa1069400 | 0.804840 | 1.780364e-49 | -8.615850e-47 |
| Msa0374040 | Msa1069400 | 0.808656 | 2.782288e-50 | -8.615850e-47 |
| Msa0405200 | Msa1069400 | 0.801569 | 8.464862e-49 | -8.615850e-47 |
| Msa0464310 | Msa1069400 | 0.804944 | 1.693892e-49 | -8.615850e-47 |
| Msa1022490 | Msa1069400 | 0.806117 | 9.611773e-50 | -8.615850e-47 |
| Msa1022510 | Msa1069400 | 0.816204 | 6.237808e-52 | -8.615850e-47 |
| Msa1064940 | Msa1069400 | 0.808122 | 3.616808e-50 | -8.615850e-47 |
| Msa1069400 | Msa1106680 | 0.803552 | 3.301100e-49 | -8.615850e-47 |
| Msa1069400 | Msa1120330 | 0.879177 | 1.540927e-69 | -8.615850e-47 |
| Msa1069400 | Msa1238250 | 0.800504 | 1.397206e-48 | -8.615850e-47 |
| Msa1069400 | Msa1319030 | 0.806139 | 9.510109e-50 | -8.615850e-47 |
| Msa1069400 | Msa1319190 | 0.807800 | 4.234848e-50 | -8.615850e-47 |
| Msa0248320 | Msa1069400 | 0.816557 | 5.201625e-52 | -8.615850e-47 |
| Msa0593870 | Msa1069400 | 0.800661 | 1.297868e-48 | -8.615850e-47 |
| Msa0854840 | Msa1069400 | 0.827631 | 1.406495e-54 | -8.615850e-47 |
| Msa0964510 | Msa1069400 | 0.808121 | 3.618472e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1069400 | MtrunA17_Chr7g0251401 | 96.923 | 130 | 4 | 0 | 1 | 130 | 1 | 130 | 3.42e-94 | 267 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCAATTCTTTGTCTCTTT+TGG | 0.156795 | 7_2:+65008978 | None:intergenic |
| CAAATTCGCATCTACTATAT+CGG | 0.330910 | 7_2:+65009116 | None:intergenic |
| TATGCTCCTCTTGGTTTCAT+AGG | 0.336107 | 7_2:+65009361 | None:intergenic |
| AGAAGCTTCATTCAGTTGAT+TGG | 0.356827 | 7_2:-65008958 | Msa1069400:CDS |
| TGAAACAAGGCAAAGGAAAA+AGG | 0.362535 | 7_2:+65008894 | None:intergenic |
| CTCTTGAGATATGCTCCTCT+TGG | 0.383087 | 7_2:+65009352 | None:intergenic |
| TGCGAATTTGAATGTTCTAA+AGG | 0.459511 | 7_2:-65009103 | Msa1069400:CDS |
| TTATGATCATTTATAGAAAG+TGG | 0.481384 | 7_2:+65009006 | None:intergenic |
| GATAGAGATGGTGAAGGTGA+AGG | 0.495568 | 7_2:-65009076 | Msa1069400:CDS |
| GGAGAGGATAGAGATGGTGA+AGG | 0.500705 | 7_2:-65009082 | Msa1069400:CDS |
| TATGATCATTTATAGAAAGT+GGG | 0.519015 | 7_2:+65009007 | None:intergenic |
| GTACAAAACTATCAAGATGA+AGG | 0.539004 | 7_2:-65009295 | Msa1069400:intron |
| AAGAGATTCAACACACGTCA+AGG | 0.556892 | 7_2:-65009334 | Msa1069400:CDS |
| GTTGCAAATGTCAACATGGT+TGG | 0.570101 | 7_2:-65009038 | Msa1069400:CDS |
| ATTTGAATGTTCTAAAGGAG+AGG | 0.592303 | 7_2:-65009098 | Msa1069400:CDS |
| TCTAAAGGAGAGGATAGAGA+TGG | 0.611605 | 7_2:-65009088 | Msa1069400:CDS |
| AGAGATTCAACACACGTCAA+GGG | 0.627969 | 7_2:-65009333 | Msa1069400:CDS |
| TTCTTACCTATGAAACCAAG+AGG | 0.628103 | 7_2:-65009367 | Msa1069400:CDS |
| CAAGCATTGAAACAAGGCAA+AGG | 0.629878 | 7_2:+65008887 | None:intergenic |
| AATGAGCAAGCATTGAAACA+AGG | 0.640206 | 7_2:+65008881 | None:intergenic |
| AGGTGAAGGAAAGATTTGAA+AGG | 0.644485 | 7_2:-65009062 | Msa1069400:CDS |
| AGGTGTTGCAAATGTCAACA+TGG | 0.719860 | 7_2:-65009042 | Msa1069400:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATCATTTATAGAAAGT+GGG | + | chr7_2:65009254-65009273 | None:intergenic | 20.0% |
| !! | TTATGATCATTTATAGAAAG+TGG | + | chr7_2:65009255-65009274 | None:intergenic | 20.0% |
| ! | AATCAACAAAAAGTAACACT+TGG | + | chr7_2:65009018-65009037 | None:intergenic | 25.0% |
| ! | AGCTATCTAATCATAATCAT+AGG | - | chr7_2:65009111-65009130 | Msa1069400:CDS | 25.0% |
| ! | GCTATCTAATCATAATCATA+GGG | - | chr7_2:65009112-65009131 | Msa1069400:CDS | 25.0% |
| GTACAAAACTATCAAGATGA+AGG | - | chr7_2:65008963-65008982 | Msa1069400:CDS | 30.0% | |
| CAAATTCGCATCTACTATAT+CGG | + | chr7_2:65009145-65009164 | None:intergenic | 30.0% | |
| TGCGAATTTGAATGTTCTAA+AGG | - | chr7_2:65009155-65009174 | Msa1069400:intron | 30.0% | |
| !! | ATTTGAATGTTCTAAAGGAG+AGG | - | chr7_2:65009160-65009179 | Msa1069400:intron | 30.0% |
| ! | TCTCAATTCTTTGTCTCTTT+TGG | + | chr7_2:65009283-65009302 | None:intergenic | 30.0% |
| !!! | CAGTTGATTGGTTTAGTTTT+TGG | - | chr7_2:65009312-65009331 | Msa1069400:CDS | 30.0% |
| !!! | GGTTTAGTTTTTGGAACTAT+TGG | - | chr7_2:65009321-65009340 | Msa1069400:CDS | 30.0% |
| TTCTTACCTATGAAACCAAG+AGG | - | chr7_2:65008891-65008910 | Msa1069400:CDS | 35.0% | |
| AGGTGAAGGAAAGATTTGAA+AGG | - | chr7_2:65009196-65009215 | Msa1069400:intron | 35.0% | |
| AGAAGCTTCATTCAGTTGAT+TGG | - | chr7_2:65009300-65009319 | Msa1069400:CDS | 35.0% | |
| TGAAACAAGGCAAAGGAAAA+AGG | + | chr7_2:65009367-65009386 | None:intergenic | 35.0% | |
| !! | AATGAGCAAGCATTGAAACA+AGG | + | chr7_2:65009380-65009399 | None:intergenic | 35.0% |
| TATGCTCCTCTTGGTTTCAT+AGG | + | chr7_2:65008900-65008919 | None:intergenic | 40.0% | |
| AAGAGATTCAACACACGTCA+AGG | - | chr7_2:65008924-65008943 | Msa1069400:CDS | 40.0% | |
| AGAGATTCAACACACGTCAA+GGG | - | chr7_2:65008925-65008944 | Msa1069400:CDS | 40.0% | |
| TCTAAAGGAGAGGATAGAGA+TGG | - | chr7_2:65009170-65009189 | Msa1069400:intron | 40.0% | |
| ! | AGGTGTTGCAAATGTCAACA+TGG | - | chr7_2:65009216-65009235 | Msa1069400:intron | 40.0% |
| GTTGCAAATGTCAACATGGT+TGG | - | chr7_2:65009220-65009239 | Msa1069400:intron | 40.0% | |
| !!! | ATTGGTCTTACCTCTTTTGC+TGG | - | chr7_2:65009339-65009358 | Msa1069400:CDS | 40.0% |
| GAAAAAGGTTCCAGCAAAAG+AGG | + | chr7_2:65009352-65009371 | None:intergenic | 40.0% | |
| !! | CAAGCATTGAAACAAGGCAA+AGG | + | chr7_2:65009374-65009393 | None:intergenic | 40.0% |
| CTCTTGAGATATGCTCCTCT+TGG | + | chr7_2:65008909-65008928 | None:intergenic | 45.0% | |
| GATAGAGATGGTGAAGGTGA+AGG | - | chr7_2:65009182-65009201 | Msa1069400:intron | 45.0% | |
| !! | ATATATTATATAGTATTATT+TGG | - | chr7_2:65009044-65009063 | Msa1069400:CDS | 5.0% |
| GGAGAGGATAGAGATGGTGA+AGG | - | chr7_2:65009176-65009195 | Msa1069400:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 65008870 | 65009410 | 65008870 | ID=Msa1069400;Name=Msa1069400 |
| chr7_2 | mRNA | 65008870 | 65009410 | 65008870 | ID=Msa1069400-mRNA-1;Parent=Msa1069400;Name=Msa1069400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|130 |
| chr7_2 | exon | 65009296 | 65009410 | 65009296 | ID=Msa1069400-mRNA-1:exon:13640;Parent=Msa1069400-mRNA-1 |
| chr7_2 | exon | 65008870 | 65009147 | 65008870 | ID=Msa1069400-mRNA-1:exon:13639;Parent=Msa1069400-mRNA-1 |
| chr7_2 | CDS | 65009296 | 65009410 | 65009296 | ID=Msa1069400-mRNA-1:cds;Parent=Msa1069400-mRNA-1 |
| chr7_2 | CDS | 65008870 | 65009147 | 65008870 | ID=Msa1069400-mRNA-1:cds;Parent=Msa1069400-mRNA-1 |
| Gene Sequence |
| Protein sequence |
