AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1100320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1100320 MtrunA17_Chr2g0322801 92.982 57 4 0 40 96 88 144 7.55e-31 110
Msa1100320 MtrunA17_Chr6g0461261 50.943 53 26 0 40 92 83 135 5.04e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1100320 AT5G08080.3 43.836 73 41 0 14 86 68 140 1.77e-14 67.8
Msa1100320 AT5G08080.5 59.574 47 19 0 40 86 83 129 7.45e-14 65.1
Msa1100320 AT5G08080.2 59.574 47 19 0 40 86 83 129 7.45e-14 65.1
Msa1100320 AT3G03800.2 53.571 56 26 0 40 95 84 139 8.14e-14 65.9
Msa1100320 AT3G03800.1 53.571 56 26 0 40 95 84 139 8.14e-14 65.9
Msa1100320 AT5G08080.1 59.574 47 19 0 40 86 83 129 9.36e-14 65.5
Msa1100320 AT5G08080.4 59.574 47 19 0 40 86 83 129 9.36e-14 65.5

Find 58 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTGAGTTGTTCTCATCT+TGG 0.296449 7_3:+29837213 None:intergenic
GTCATTGACCCATGATTTGA+TGG 0.313345 7_3:+29837130 None:intergenic
CCATTGATGGACTTGAGATA+TGG 0.314818 7_3:-29837157 Msa1100320:three_prime_UTR
GGGGATCTTGACAGAGTTCT+AGG 0.330805 7_3:-29839364 Msa1100320:intron
TTTGAGTAGAAGTTCGATAA+TGG 0.338883 7_3:-29838752 Msa1100320:three_prime_UTR
GTGGAGAGAAGAGTTATTAC+AGG 0.369427 7_3:-29839223 Msa1100320:intron
AATGAAAGTTGAGATCCTTT+TGG 0.372927 7_3:-29838982 Msa1100320:three_prime_UTR
CAATGACTTAAAAGATGTTT+AGG 0.377845 7_3:-29837114 Msa1100320:three_prime_UTR
CAAGATGAGAACAACTCAAC+TGG 0.392091 7_3:-29837212 Msa1100320:three_prime_UTR
GACTCTCCAAAGGCCATTGA+TGG 0.397359 7_3:-29837170 Msa1100320:three_prime_UTR
TCAGTTGACTATCATGAATC+AGG 0.415010 7_3:-29840084 None:intergenic
CATTGATGGACTTGAGATAT+GGG 0.415248 7_3:-29837156 Msa1100320:three_prime_UTR
TCAAGTCCATCAATGGCCTT+TGG 0.439526 7_3:+29837164 None:intergenic
ATTACATGTTCAAACCTTTG+TGG 0.443048 7_3:+29838894 None:intergenic
ATTTAATGTGTCTGATACCC+TGG 0.462090 7_3:+29837063 None:intergenic
CATTCTTGCTCCGTCAATAC+CGG 0.466523 7_3:+29839502 None:intergenic
ATCTACTTTGATATGACTCA+TGG 0.466990 7_3:-29838935 Msa1100320:three_prime_UTR
ATTGTAGCAGTCCCTCTGGC+AGG 0.468310 7_3:-29840041 Msa1100320:CDS
GCAGAATAAGTGTGACCAAA+AGG 0.486214 7_3:+29838967 None:intergenic
GAAAAGGATATTGATGAGGT+TGG 0.486934 7_3:-29839716 Msa1100320:intron
TTTCAGATTCGTACCTTCCT+CGG 0.487984 7_3:+29839960 None:intergenic
TATGGGTTTCCATCAAATCA+TGG 0.491136 7_3:-29837139 Msa1100320:three_prime_UTR
AGATGAATATCGTGAGGTTG+TGG 0.491519 7_3:-29839242 Msa1100320:exon
CCATATCTCAAGTCCATCAA+TGG 0.492238 7_3:+29837157 None:intergenic
CAAAAGCCTGGCTGTGAGAA+GGG 0.499127 7_3:-29839527 Msa1100320:CDS
CCGGTTCCCTTCTCACAGCC+AGG 0.501105 7_3:+29839521 None:intergenic
ATATTTCACATATTATACTC+TGG 0.501147 7_3:+29837091 None:intergenic
CCTGGCTGTGAGAAGGGAAC+CGG 0.506291 7_3:-29839521 Msa1100320:CDS
CTGCTACAATCCACGCCAGC+AGG 0.509313 7_3:+29840054 None:intergenic
GAGAAGGGAACCGGTATTGA+CGG 0.524006 7_3:-29839512 Msa1100320:CDS
CTAAACAATAGACAAAAGCC+TGG 0.535553 7_3:-29839539 Msa1100320:CDS
ACAAAAGCCTGGCTGTGAGA+AGG 0.542434 7_3:-29839528 Msa1100320:CDS
ATATTTGACTTCTCCGAGGA+AGG 0.549457 7_3:-29839973 Msa1100320:intron
AGGAGAAGTTCCTGCTGGCG+TGG 0.553063 7_3:-29840064 Msa1100320:CDS
CAAAATATATTTATGATGAG+TGG 0.560071 7_3:-29838727 Msa1100320:three_prime_UTR
GTGGATTGTAGCAGTCCCTC+TGG 0.561812 7_3:-29840045 Msa1100320:CDS
ATGGGTTTCCATCAAATCAT+GGG 0.572571 7_3:-29837138 Msa1100320:three_prime_UTR
ATCAGATTTCTCCCTGCCAG+AGG 0.577582 7_3:+29840029 None:intergenic
TCTACTTTGATATGACTCAT+GGG 0.577770 7_3:-29838934 Msa1100320:three_prime_UTR
ATTTATGATGAGTGGTGAAG+AGG 0.585867 7_3:-29838719 Msa1100320:intron
ATATGTGAAATATATAAACC+AGG 0.586785 7_3:-29837081 Msa1100320:three_prime_UTR
TCAGATTTCTCCCTGCCAGA+GGG 0.589490 7_3:+29840030 None:intergenic
GAATCAGGAGAAGTTCCTGC+TGG 0.602112 7_3:-29840069 Msa1100320:CDS
TTGTAGCAGTCCCTCTGGCA+GGG 0.602924 7_3:-29840040 Msa1100320:CDS
TGGGACTCAACATCTCATGT+TGG 0.604260 7_3:-29837192 Msa1100320:three_prime_UTR
TAGAACTCTGTCAAGATCCC+CGG 0.605228 7_3:+29839366 None:intergenic
GGTTGGAAAGATTGCACATG+GGG 0.615837 7_3:-29839699 Msa1100320:CDS
TATGTGAAATATATAAACCA+GGG 0.618460 7_3:-29837080 Msa1100320:three_prime_UTR
GGTGATAATGATGACATGCT+TGG 0.621093 7_3:-29840000 Msa1100320:CDS
AACTTGATATTGCAGATGCA+TGG 0.621769 7_3:-29837243 Msa1100320:intron
TCTCATGTTGGACTCTCCAA+AGG 0.622034 7_3:-29837180 Msa1100320:three_prime_UTR
AGGGAGAAATCTGATGAAGA+TGG 0.631588 7_3:-29840021 Msa1100320:CDS
AAGATGAGAACAACTCAACT+GGG 0.640277 7_3:-29837211 Msa1100320:three_prime_UTR
GATGGAAAAGGATATTGATG+AGG 0.655016 7_3:-29839720 Msa1100320:intron
GAGGTTGGAAAGATTGCACA+TGG 0.661524 7_3:-29839701 Msa1100320:CDS
AATACAAGATGAATATCGTG+AGG 0.666598 7_3:-29839248 Msa1100320:exon
TGATATATTTGACTTCTCCG+AGG 0.718503 7_3:-29839977 Msa1100320:CDS
AGGTTGGAAAGATTGCACAT+GGG 0.720039 7_3:-29839700 Msa1100320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATCTAAACAAAATTAAA+AGG + chr7_3:29837167-29837186 None:intergenic 15.0%
!!! TTTACATTGATTGATTTTAT+TGG - chr7_3:29838243-29838262 Msa1100320:intron 15.0%
!!! TTACATTGATTGATTTTATT+GGG - chr7_3:29838244-29838263 Msa1100320:intron 15.0%
!! AAATACCATTATGATTTATA+TGG - chr7_3:29839464-29839483 Msa1100320:intron 15.0%
!!! TAATGCTCATAATATTTTAC+TGG - chr7_3:29837320-29837339 Msa1100320:intron 20.0%
!!! TCATAATATTTTACTGGTAT+TGG - chr7_3:29837326-29837345 Msa1100320:intron 20.0%
!! TGTTATATAGATTCCAAATA+CGG - chr7_3:29837494-29837513 Msa1100320:intron 20.0%
!!! AACTATTTTTTATCCGTATT+TGG + chr7_3:29837510-29837529 None:intergenic 20.0%
!!! ACTTTGTATATCATGAATTT+TGG + chr7_3:29837984-29838003 None:intergenic 20.0%
!! AAGTTTACATTTCAAAATAC+AGG + chr7_3:29838081-29838100 None:intergenic 20.0%
!! CAAAATATATTTATGATGAG+TGG - chr7_3:29838367-29838386 Msa1100320:intron 20.0%
!! AGAATTTAATACATAGAGAA+GGG + chr7_3:29838430-29838449 None:intergenic 20.0%
!! TAGAATTTAATACATAGAGA+AGG + chr7_3:29838431-29838450 None:intergenic 20.0%
!! CTTCTGTTTAATAATTTCAA+GGG - chr7_3:29838609-29838628 Msa1100320:intron 20.0%
!!! TTCTTTTTCATTTTCTGTTA+TGG - chr7_3:29838965-29838984 Msa1100320:three_prime_UTR 20.0%
!!! CCATTTGCATTTTTTTTAAA+GGG + chr7_3:29839263-29839282 None:intergenic 20.0%
!!! CCTTTAAAAAAAATGCAAAT+GGG - chr7_3:29839261-29839280 Msa1100320:intron 20.0%
!!! CTTTAAAAAAAATGCAAATG+GGG - chr7_3:29839262-29839281 Msa1100320:intron 20.0%
!!! TAAATTTTCATACCATGATA+TGG - chr7_3:29839429-29839448 Msa1100320:intron 20.0%
!! AATGACCATATAAATCATAA+TGG + chr7_3:29839472-29839491 None:intergenic 20.0%
!! ATTAAAAGATTTGAGTGATA+TGG + chr7_3:29839517-29839536 None:intergenic 20.0%
!! AAAAATGTAATCAACCATTT+AGG - chr7_3:29839693-29839712 Msa1100320:CDS 20.0%
!! ATATTTCACATATTATACTC+TGG + chr7_3:29840006-29840025 None:intergenic 20.0%
!! ATATGTGAAATATATAAACC+AGG - chr7_3:29840013-29840032 Msa1100320:CDS 20.0%
!! TATGTGAAATATATAAACCA+GGG - chr7_3:29840014-29840033 Msa1100320:CDS 20.0%
! ACGGATAAAAAATAGTTAAC+CGG - chr7_3:29837513-29837532 Msa1100320:intron 25.0%
! AACACAGTTAACTTTATCAA+GGG + chr7_3:29837912-29837931 None:intergenic 25.0%
! TAAAATTCATGATATGCACA+AGG - chr7_3:29838033-29838052 Msa1100320:intron 25.0%
!! TGAAATGTAAACTTTGAGAA+AGG - chr7_3:29838087-29838106 Msa1100320:intron 25.0%
!! AAGGTTTGAACATGTAATTT+TGG - chr7_3:29838205-29838224 Msa1100320:intron 25.0%
! GCTTCTGTTTAATAATTTCA+AGG - chr7_3:29838608-29838627 Msa1100320:intron 25.0%
! TTTAATAATTTCAAGGGATG+TGG - chr7_3:29838615-29838634 Msa1100320:intron 25.0%
!! ATAATAGAGTGTTTAATAGC+CGG - chr7_3:29838657-29838676 Msa1100320:intron 25.0%
!! CCCTTTAAAAAAAATGCAAA+TGG - chr7_3:29839260-29839279 Msa1100320:intron 25.0%
!!! CCCATTTGCATTTTTTTTAA+AGG + chr7_3:29839264-29839283 None:intergenic 25.0%
!! TTTTTATCAAGACCATATCA+TGG + chr7_3:29839444-29839463 None:intergenic 25.0%
!! CAAATCTTTTAATCCAACAA+TGG - chr7_3:29839524-29839543 Msa1100320:CDS 25.0%
!! TCAGATTTTATAACCATTGT+TGG + chr7_3:29839540-29839559 None:intergenic 25.0%
! AATCTGAGTCAAAAAAACAA+TGG - chr7_3:29839553-29839572 Msa1100320:intron 25.0%
! TAAAATCGAGATTCAAATCA+TGG - chr7_3:29839579-29839598 Msa1100320:intron 25.0%
! TCACTATAAAATTCGATTCA+CGG + chr7_3:29839625-29839644 None:intergenic 25.0%
! ACAATGAATTACTACCTAAA+TGG + chr7_3:29839710-29839729 None:intergenic 25.0%
! TAGGTAGTAATTCATTGTAA+TGG - chr7_3:29839712-29839731 Msa1100320:CDS 25.0%
! ATCTCTAATGTGTATCTATT+CGG + chr7_3:29839763-29839782 None:intergenic 25.0%
! CAATGACTTAAAAGATGTTT+AGG - chr7_3:29839980-29839999 Msa1100320:CDS 25.0%
CTGAATCATAACAATTGTCT+TGG - chr7_3:29837352-29837371 Msa1100320:intron 30.0%
ATCATAACAATTGTCTTGGA+TGG - chr7_3:29837356-29837375 Msa1100320:intron 30.0%
CGGATAAAAAATAGTTAACC+GGG - chr7_3:29837514-29837533 Msa1100320:intron 30.0%
ATTCTGCATAAAACATATCC+CGG + chr7_3:29837535-29837554 None:intergenic 30.0%
!!! GAAAAACAGCTTCTAGTTTT+AGG + chr7_3:29837773-29837792 None:intergenic 30.0%
TGAGAAGATAGACATAAACT+TGG + chr7_3:29837795-29837814 None:intergenic 30.0%
AATACAAGATGAATATCGTG+AGG - chr7_3:29837846-29837865 Msa1100320:intron 30.0%
ACACAGTTAACTTTATCAAG+GGG + chr7_3:29837911-29837930 None:intergenic 30.0%
CAACACAGTTAACTTTATCA+AGG + chr7_3:29837913-29837932 None:intergenic 30.0%
GTAGTACTATAGAAATTTGC+TGG - chr7_3:29837940-29837959 Msa1100320:intron 30.0%
! AATGAAAGTTGAGATCCTTT+TGG - chr7_3:29838112-29838131 Msa1100320:intron 30.0%
ATCTACTTTGATATGACTCA+TGG - chr7_3:29838159-29838178 Msa1100320:intron 30.0%
TCTACTTTGATATGACTCAT+GGG - chr7_3:29838160-29838179 Msa1100320:intron 30.0%
ATTACATGTTCAAACCTTTG+TGG + chr7_3:29838203-29838222 None:intergenic 30.0%
TGCACTTAGTAACAACAAAA+GGG + chr7_3:29838281-29838300 None:intergenic 30.0%
TTTGAGTAGAAGTTCGATAA+TGG - chr7_3:29838342-29838361 Msa1100320:intron 30.0%
TTCTAAACACCCAAACAAAT+AGG - chr7_3:29838402-29838421 Msa1100320:intron 30.0%
!! TTTGTGATTAGTTTGAGCTA+CGG - chr7_3:29839050-29839069 Msa1100320:intron 30.0%
!!! TTTAGCTTTTGCTTCTGTTA+TGG - chr7_3:29839148-29839167 Msa1100320:intron 30.0%
!! TTAGCTTTTGCTTCTGTTAT+GGG - chr7_3:29839149-29839168 Msa1100320:intron 30.0%
ACTCTTTAATAGAGATCAGT+AGG + chr7_3:29839321-29839340 None:intergenic 30.0%
TACTGATCTCTATTAAAGAG+TGG - chr7_3:29839320-29839339 Msa1100320:intron 30.0%
! ATTAGAGATTCATGTCGATT+GGG - chr7_3:29839774-29839793 Msa1100320:intron 30.0%
! TGATATATTTGACTTCTCCG+AGG - chr7_3:29837117-29837136 Msa1100320:three_prime_UTR 35.0%
!! ATAAAAGCATCTGCCATGAA+AGG - chr7_3:29837245-29837264 Msa1100320:intron 35.0%
CCTTGTAACAATACCTTTCA+TGG + chr7_3:29837261-29837280 None:intergenic 35.0%
!! CCATGAAAGGTATTGTTACA+AGG - chr7_3:29837258-29837277 Msa1100320:intron 35.0%
! GGCAATGTCTGTAATTCTTT+TGG - chr7_3:29837279-29837298 Msa1100320:intron 35.0%
ACAATTGTCTTGGATGGAAA+AGG - chr7_3:29837362-29837381 Msa1100320:intron 35.0%
GATGGAAAAGGATATTGATG+AGG - chr7_3:29837374-29837393 Msa1100320:intron 35.0%
GAAAAGGATATTGATGAGGT+TGG - chr7_3:29837378-29837397 Msa1100320:intron 35.0%
CTAAACAATAGACAAAAGCC+TGG - chr7_3:29837555-29837574 Msa1100320:intron 35.0%
AGTTCCTTGACTAAAAAGTC+CGG - chr7_3:29837709-29837728 Msa1100320:intron 35.0%
AAGTTAACTGTGTTGCACTA+CGG - chr7_3:29837918-29837937 Msa1100320:intron 35.0%
CTGCACTTAGTAACAACAAA+AGG + chr7_3:29838282-29838301 None:intergenic 35.0%
!!! CTTTTGTTGTTACTAAGTGC+AGG - chr7_3:29838280-29838299 Msa1100320:intron 35.0%
ATTTATGATGAGTGGTGAAG+AGG - chr7_3:29838375-29838394 Msa1100320:intron 35.0%
AGAAGGGATCCTATTTGTTT+GGG + chr7_3:29838414-29838433 None:intergenic 35.0%
! ATGATATCAACGTTTTGTCG+TGG - chr7_3:29838705-29838724 Msa1100320:intron 35.0%
CATTCATAAAACAGACACAG+AGG + chr7_3:29838758-29838777 None:intergenic 35.0%
! CTCTGTGTCTGTTTTATGAA+TGG - chr7_3:29838756-29838775 Msa1100320:three_prime_UTR 35.0%
GAAAAATAGCTGCTGCTAAT+TGG + chr7_3:29838869-29838888 None:intergenic 35.0%
! TCATTTTCTGTTATGGACTC+TGG - chr7_3:29838972-29838991 Msa1100320:three_prime_UTR 35.0%
! CATTTTCTGTTATGGACTCT+GGG - chr7_3:29838973-29838992 Msa1100320:three_prime_UTR 35.0%
! TTTTGCTTCTGTTATGGGTT+GGG - chr7_3:29839154-29839173 Msa1100320:intron 35.0%
! GGACGAAAGAAAAGTACAAT+AGG + chr7_3:29839200-29839219 None:intergenic 35.0%
GAGTGGAAATTTACTGAACA+AGG - chr7_3:29839337-29839356 Msa1100320:intron 35.0%
TTCCTGCTGATCTTATCAAA+AGG - chr7_3:29839362-29839381 Msa1100320:intron 35.0%
! TACCTTTTGATAAGATCAGC+AGG + chr7_3:29839367-29839386 None:intergenic 35.0%
TTGAGTGATATGGTAGAAGT+GGG + chr7_3:29839507-29839526 None:intergenic 35.0%
TTTGAGTGATATGGTAGAAG+TGG + chr7_3:29839508-29839527 None:intergenic 35.0%
TCGAGATTCAAATCATGGAT+TGG - chr7_3:29839584-29839603 Msa1100320:intron 35.0%
!! AGATTCAAATCATGGATTGG+AGG - chr7_3:29839587-29839606 Msa1100320:intron 35.0%
TGTAACACTGCCAATAAAAG+TGG - chr7_3:29839647-29839666 Msa1100320:intron 35.0%
!!! GAATAACCAGCCACTTTTAT+TGG + chr7_3:29839660-29839679 None:intergenic 35.0%
CATTAGAGATTCATGTCGAT+TGG - chr7_3:29839773-29839792 Msa1100320:intron 35.0%
AACTTGATATTGCAGATGCA+TGG - chr7_3:29839851-29839870 Msa1100320:intron 35.0%
AAGATGAGAACAACTCAACT+GGG - chr7_3:29839883-29839902 Msa1100320:intron 35.0%
!! CATTGATGGACTTGAGATAT+GGG - chr7_3:29839938-29839957 Msa1100320:intron 35.0%
TATGGGTTTCCATCAAATCA+TGG - chr7_3:29839955-29839974 Msa1100320:intron 35.0%
ATGGGTTTCCATCAAATCAT+GGG - chr7_3:29839956-29839975 Msa1100320:intron 35.0%
ATTTAATGTGTCTGATACCC+TGG + chr7_3:29840034-29840053 None:intergenic 35.0%
AGGGAGAAATCTGATGAAGA+TGG - chr7_3:29837073-29837092 Msa1100320:three_prime_UTR 40.0%
! GGTGATAATGATGACATGCT+TGG - chr7_3:29837094-29837113 Msa1100320:three_prime_UTR 40.0%
! ATATTTGACTTCTCCGAGGA+AGG - chr7_3:29837121-29837140 Msa1100320:three_prime_UTR 40.0%
TTTCAGATTCGTACCTTCCT+CGG + chr7_3:29837137-29837156 None:intergenic 40.0%
! ATAGCAATAGCACTTTTCGC+AGG - chr7_3:29837196-29837215 Msa1100320:three_prime_UTR 40.0%
AGGTTGGAAAGATTGCACAT+GGG - chr7_3:29837394-29837413 Msa1100320:intron 40.0%
GTCAAGGAACTGCAATTTCA+AGG + chr7_3:29837700-29837719 None:intergenic 40.0%
GTTCCTTGACTAAAAAGTCC+GGG - chr7_3:29837710-29837729 Msa1100320:intron 40.0%
TTCCTTGACTAAAAAGTCCG+GGG - chr7_3:29837711-29837730 Msa1100320:intron 40.0%
!! TGACAGAGTTCTAGGTTTGT+TGG - chr7_3:29837738-29837757 Msa1100320:intron 40.0%
AGATGAATATCGTGAGGTTG+TGG - chr7_3:29837852-29837871 Msa1100320:intron 40.0%
GTGGAGAGAAGAGTTATTAC+AGG - chr7_3:29837871-29837890 Msa1100320:intron 40.0%
GCAGAATAAGTGTGACCAAA+AGG + chr7_3:29838130-29838149 None:intergenic 40.0%
! CATGGGATGAGATAGCTTTT+TGG - chr7_3:29838177-29838196 Msa1100320:intron 40.0%
!! AGATAGCTTTTTGGCCACAA+AGG - chr7_3:29838186-29838205 Msa1100320:intron 40.0%
GAGAAGGGATCCTATTTGTT+TGG + chr7_3:29838415-29838434 None:intergenic 40.0%
! TCTTTTCCAAGCTGAGATCA+AGG - chr7_3:29838574-29838593 Msa1100320:intron 40.0%
! CTTTTCCAAGCTGAGATCAA+GGG - chr7_3:29838575-29838594 Msa1100320:intron 40.0%
TTATTCCCTTGATCTCAGCT+TGG + chr7_3:29838583-29838602 None:intergenic 40.0%
TGTGGATGTTGTGAATTGCT+AGG - chr7_3:29838633-29838652 Msa1100320:intron 40.0%
TCCACAATTTCATCGAGATC+CGG + chr7_3:29838686-29838705 None:intergenic 40.0%
TTAACTGTTTGGAGGTTGTC+TGG - chr7_3:29838814-29838833 Msa1100320:intron 40.0%
TTAGGGTCTTTCGGTTGTTT+GGG - chr7_3:29839023-29839042 Msa1100320:intron 40.0%
! ACTTGCTTCTGTCGCATTTT+AGG - chr7_3:29839115-29839134 Msa1100320:intron 40.0%
! CTTGCTTCTGTCGCATTTTA+GGG - chr7_3:29839116-29839135 Msa1100320:intron 40.0%
! TTGCTTCTGTCGCATTTTAG+GGG - chr7_3:29839117-29839136 Msa1100320:intron 40.0%
! CTTTTGCTTCTGTTATGGGT+TGG - chr7_3:29839153-29839172 Msa1100320:intron 40.0%
TTGGGTTTACCGTCTATATG+AGG - chr7_3:29839172-29839191 Msa1100320:intron 40.0%
CAATAGGTGCCTCATATAGA+CGG + chr7_3:29839184-29839203 None:intergenic 40.0%
! CAGTTGAGTTGTTCTCATCT+TGG + chr7_3:29839884-29839903 None:intergenic 40.0%
CAAGATGAGAACAACTCAAC+TGG - chr7_3:29839882-29839901 Msa1100320:intron 40.0%
CCATATCTCAAGTCCATCAA+TGG + chr7_3:29839940-29839959 None:intergenic 40.0%
!! CCATTGATGGACTTGAGATA+TGG - chr7_3:29839937-29839956 Msa1100320:intron 40.0%
GTCATTGACCCATGATTTGA+TGG + chr7_3:29839967-29839986 None:intergenic 40.0%
TGCGCAAAAACAAGCAAACA+TGG + chr7_3:29840074-29840093 None:intergenic 40.0%
! CTTTTCGCAGGAAGCTAATG+AGG - chr7_3:29837208-29837227 Msa1100320:three_prime_UTR 45.0%
GAGGTTGGAAAGATTGCACA+TGG - chr7_3:29837393-29837412 Msa1100320:intron 45.0%
GGTTGGAAAGATTGCACATG+GGG - chr7_3:29837395-29837414 Msa1100320:intron 45.0%
CATTCTTGCTCCGTCAATAC+CGG + chr7_3:29837595-29837614 None:intergenic 45.0%
!! ATCCCCGGACTTTTTAGTCA+AGG + chr7_3:29837716-29837735 None:intergenic 45.0%
TAGAACTCTGTCAAGATCCC+CGG + chr7_3:29837731-29837750 None:intergenic 45.0%
AGTGTTTAATAGCCGGAACC+CGG - chr7_3:29838664-29838683 Msa1100320:intron 45.0%
CCACAATTTCATCGAGATCC+GGG + chr7_3:29838685-29838704 None:intergenic 45.0%
! ATGAATGGTGTTAGTGCCCT+AGG - chr7_3:29838771-29838790 Msa1100320:three_prime_UTR 45.0%
! TGAATGGTGTTAGTGCCCTA+GGG - chr7_3:29838772-29838791 Msa1100320:three_prime_UTR 45.0%
!! TTGCGGCTGGTTTAACTGTT+TGG - chr7_3:29838803-29838822 Msa1100320:intron 45.0%
ACAACAACACAACAGGTCCA+CGG + chr7_3:29838905-29838924 None:intergenic 45.0%
TACGGACACAACAACACAAC+AGG + chr7_3:29838912-29838931 None:intergenic 45.0%
ATGGACTCTGGGTGTATTTG+CGG - chr7_3:29838984-29839003 Msa1100320:three_prime_UTR 45.0%
GTGTATTTGCGGGTGGTTTA+TGG - chr7_3:29838995-29839014 Msa1100320:intron 45.0%
TATTTGCGGGTGGTTTATGG+AGG - chr7_3:29838998-29839017 Msa1100320:intron 45.0%
GGTGGTTTATGGAGGTCTTA+GGG - chr7_3:29839006-29839025 Msa1100320:intron 45.0%
ATGGAGGTCTTAGGGTCTTT+CGG - chr7_3:29839014-29839033 Msa1100320:intron 45.0%
CTTAGGGTCTTTCGGTTGTT+TGG - chr7_3:29839022-29839041 Msa1100320:intron 45.0%
TGAGCTACGGTTGTGTATTG+TGG - chr7_3:29839063-29839082 Msa1100320:intron 45.0%
!! TCTGTCGCATTTTAGGGGTT+TGG - chr7_3:29839122-29839141 Msa1100320:intron 45.0%
GGGTATGTGCTTATGCATCA+AGG - chr7_3:29839282-29839301 Msa1100320:intron 45.0%
GATATGGTAGAAGTGGGAGA+GGG + chr7_3:29839501-29839520 None:intergenic 45.0%
TGATATGGTAGAAGTGGGAG+AGG + chr7_3:29839502-29839521 None:intergenic 45.0%
ACACTGCCAATAAAAGTGGC+TGG - chr7_3:29839651-29839670 Msa1100320:intron 45.0%
TGGGACTCAACATCTCATGT+TGG - chr7_3:29839902-29839921 Msa1100320:intron 45.0%
TCTCATGTTGGACTCTCCAA+AGG - chr7_3:29839914-29839933 Msa1100320:intron 45.0%
TCAAGTCCATCAATGGCCTT+TGG + chr7_3:29839933-29839952 None:intergenic 45.0%
GAATCAGGAGAAGTTCCTGC+TGG - chr7_3:29837025-29837044 Msa1100320:three_prime_UTR 50.0%
TCAGATTTCTCCCTGCCAGA+GGG + chr7_3:29837067-29837086 None:intergenic 50.0%
ATCAGATTTCTCCCTGCCAG+AGG + chr7_3:29837068-29837087 None:intergenic 50.0%
ACAAAAGCCTGGCTGTGAGA+AGG - chr7_3:29837566-29837585 Msa1100320:intron 50.0%
CAAAAGCCTGGCTGTGAGAA+GGG - chr7_3:29837567-29837586 Msa1100320:intron 50.0%
! GAGAAGGGAACCGGTATTGA+CGG - chr7_3:29837582-29837601 Msa1100320:intron 50.0%
GGGGATCTTGACAGAGTTCT+AGG - chr7_3:29837730-29837749 Msa1100320:intron 50.0%
TTTCATCGAGATCCGGGTTC+CGG + chr7_3:29838679-29838698 None:intergenic 50.0%
CCCGGATCTCGATGAAATTG+TGG - chr7_3:29838682-29838701 Msa1100320:intron 50.0%
! TCAACGTTTTGTCGTGGCGA+TGG - chr7_3:29838711-29838730 Msa1100320:intron 50.0%
TTTGTCGTGGCGATGGTCTT+TGG - chr7_3:29838718-29838737 Msa1100320:intron 50.0%
! GCCCTAGGGAATGTCTTTTG+CGG - chr7_3:29838786-29838805 Msa1100320:three_prime_UTR 50.0%
GCCGCAAAAGACATTCCCTA+GGG + chr7_3:29838790-29838809 None:intergenic 50.0%
AGCCGCAAAAGACATTCCCT+AGG + chr7_3:29838791-29838810 None:intergenic 50.0%
! TAGGGAATGTCTTTTGCGGC+TGG - chr7_3:29838790-29838809 Msa1100320:three_prime_UTR 50.0%
!! CGGCTGGTTTAACTGTTTGG+AGG - chr7_3:29838806-29838825 Msa1100320:intron 50.0%
TTTGGAGGTTGTCTGGCGTA+AGG - chr7_3:29838821-29838840 Msa1100320:intron 50.0%
TTGGAGGTTGTCTGGCGTAA+GGG - chr7_3:29838822-29838841 Msa1100320:intron 50.0%
TTGTGTCCGTATAGCTGCTG+AGG - chr7_3:29838921-29838940 Msa1100320:three_prime_UTR 50.0%
TGTGTCCGTATAGCTGCTGA+GGG - chr7_3:29838922-29838941 Msa1100320:three_prime_UTR 50.0%
TGGACTCTGGGTGTATTTGC+GGG - chr7_3:29838985-29839004 Msa1100320:three_prime_UTR 50.0%
GGGTGGTTTATGGAGGTCTT+AGG - chr7_3:29839005-29839024 Msa1100320:intron 50.0%
!! GTCGCATTTTAGGGGTTTGG+AGG - chr7_3:29839125-29839144 Msa1100320:intron 50.0%
GACTCTCCAAAGGCCATTGA+TGG - chr7_3:29839924-29839943 Msa1100320:intron 50.0%
! GTGGATTGTAGCAGTCCCTC+TGG - chr7_3:29837049-29837068 Msa1100320:three_prime_UTR 55.0%
ATTGTAGCAGTCCCTCTGGC+AGG - chr7_3:29837053-29837072 Msa1100320:three_prime_UTR 55.0%
TTGTAGCAGTCCCTCTGGCA+GGG - chr7_3:29837054-29837073 Msa1100320:three_prime_UTR 55.0%
GTTGTCTGGCGTAAGGGAGA+GGG - chr7_3:29838828-29838847 Msa1100320:intron 55.0%
TTGTCTGGCGTAAGGGAGAG+GGG - chr7_3:29838829-29838848 Msa1100320:intron 55.0%
TTTCTCTGCTGCTGTGACCG+TGG - chr7_3:29838885-29838904 Msa1100320:intron 55.0%
GTGTCCGTATAGCTGCTGAG+GGG - chr7_3:29838923-29838942 Msa1100320:three_prime_UTR 55.0%
ACTCTGGGTGTATTTGCGGG+TGG - chr7_3:29838988-29839007 Msa1100320:three_prime_UTR 55.0%
TCCATTCCTTGCGTCTTGCG+AGG - chr7_3:29839217-29839236 Msa1100320:intron 55.0%
CCATTCCTTGCGTCTTGCGA+GGG - chr7_3:29839218-29839237 Msa1100320:intron 55.0%
AGGAGAAGTTCCTGCTGGCG+TGG - chr7_3:29837030-29837049 Msa1100320:three_prime_UTR 60.0%
CTGCTACAATCCACGCCAGC+AGG + chr7_3:29837043-29837062 None:intergenic 60.0%
CCTGGCTGTGAGAAGGGAAC+CGG - chr7_3:29837573-29837592 Msa1100320:intron 60.0%
GGTTGTCTGGCGTAAGGGAG+AGG - chr7_3:29838827-29838846 Msa1100320:intron 60.0%
TGTCTGGCGTAAGGGAGAGG+GGG - chr7_3:29838830-29838849 Msa1100320:intron 60.0%
GCTGCCCCTCAGCAGCTATA+CGG + chr7_3:29838930-29838949 None:intergenic 60.0%
CCCTCGCAAGACGCAAGGAA+TGG + chr7_3:29839221-29839240 None:intergenic 60.0%
TTCCTTGCGTCTTGCGAGGG+TGG - chr7_3:29839221-29839240 Msa1100320:intron 60.0%
CCGGTTCCCTTCTCACAGCC+AGG + chr7_3:29837576-29837595 None:intergenic 65.0%
AAGGGAGAGGGGGCTGCTTC+TGG - chr7_3:29838840-29838859 Msa1100320:intron 65.0%
CGCCACCCTCGCAAGACGCA+AGG + chr7_3:29839226-29839245 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 29837023 29840093 29837023 ID=Msa1100320;Name=Msa1100320
chr7_3 mRNA 29837023 29840093 29837023 ID=Msa1100320-mRNA-1;Parent=Msa1100320;Name=Msa1100320-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|0.85|0.87|0.87|0.28|0.5|8|457|106
chr7_3 exon 29837023 29837250 29837023 ID=Msa1100320-mRNA-1:exon:6395;Parent=Msa1100320-mRNA-1
chr7_3 exon 29838720 29838814 29838720 ID=Msa1100320-mRNA-1:exon:6394;Parent=Msa1100320-mRNA-1
chr7_3 exon 29838909 29839007 29838909 ID=Msa1100320-mRNA-1:exon:6393;Parent=Msa1100320-mRNA-1
chr7_3 exon 29839224 29839278 29839224 ID=Msa1100320-mRNA-1:exon:6392;Parent=Msa1100320-mRNA-1
chr7_3 exon 29839365 29839405 29839365 ID=Msa1100320-mRNA-1:exon:6391;Parent=Msa1100320-mRNA-1
chr7_3 exon 29839491 29839564 29839491 ID=Msa1100320-mRNA-1:exon:6390;Parent=Msa1100320-mRNA-1
chr7_3 exon 29839667 29839732 29839667 ID=Msa1100320-mRNA-1:exon:6389;Parent=Msa1100320-mRNA-1
chr7_3 exon 29839974 29840093 29839974 ID=Msa1100320-mRNA-1:exon:6388;Parent=Msa1100320-mRNA-1
chr7_3 CDS 29839974 29840093 29839974 ID=Msa1100320-mRNA-1:cds;Parent=Msa1100320-mRNA-1
chr7_3 CDS 29839667 29839732 29839667 ID=Msa1100320-mRNA-1:cds;Parent=Msa1100320-mRNA-1
chr7_3 CDS 29839491 29839564 29839491 ID=Msa1100320-mRNA-1:cds;Parent=Msa1100320-mRNA-1
chr7_3 CDS 29839365 29839405 29839365 ID=Msa1100320-mRNA-1:cds;Parent=Msa1100320-mRNA-1
chr7_3 CDS 29839259 29839278 29839259 ID=Msa1100320-mRNA-1:cds;Parent=Msa1100320-mRNA-1
chr7_3 three_prime_UTR 29839224 29839258 29839224 ID=Msa1100320-mRNA-1:three_prime_utr;Parent=Msa1100320-mRNA-1
chr7_3 three_prime_UTR 29838909 29839007 29838909 ID=Msa1100320-mRNA-1:three_prime_utr;Parent=Msa1100320-mRNA-1
chr7_3 three_prime_UTR 29838720 29838814 29838720 ID=Msa1100320-mRNA-1:three_prime_utr;Parent=Msa1100320-mRNA-1
chr7_3 three_prime_UTR 29837023 29837250 29837023 ID=Msa1100320-mRNA-1:three_prime_utr;Parent=Msa1100320-mRNA-1
Gene Sequence

>Msa1100320

ATGAATCAGGAGAAGTTCCTGCTGGCGTGGATTGTAGCAGTCCCTCTGGCAGGGAGAAATCTGATGAAGATGGTGATAATGATGACATGCTTGGTGATATATTTGACTTCTCCGAGGAAGGATATTGATGAGGTTGGAAAGATTGCACATGGGGTCAAAGCAAAAATTGAATCTATCAACAGAGAAAATCTAAACAATAGACAAAAGCCTGGCTGTGAGAAGGGAACCGGTATTGACGGAGCAAGAATGAATATGACAAATTCCTTGACTAAAAAGTCCGGGGATCTTGACAGAGTTCTAGAGACAAAGAATACAAGATGA
Protein sequence

>Msa1100320

MNQEKFLLAWIVAVPLAGRNLMKMVIMMTCLVIYLTSPRKDIDEVGKIAHGVKAKIESINRENLNNRQKPGCEKGTGIDGARMNMTNSLTKKSGDLDRVLETKNTR*