AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0432300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0432300 MtrunA17_Chr8g0356491 42.683 164 87 6 50 210 5 164 2.11e-25 103
Msa0432300 MtrunA17_Chr5g0397151 39.873 158 83 5 58 210 17 167 2.66e-24 100
Msa0432300 MtrunA17_Chr1g0173981 37.736 159 63 8 71 210 19 160 2.66e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGACCCTAATTCATATT+TGG 0.186549 3_2:-84483341 None:intergenic
GTCAAGAATGCATGTAAATT+TGG 0.231122 3_2:+84483170 Msa0432300:CDS
AAATTTGGTTATCCTATAAA+AGG 0.245445 3_2:+84482775 Msa0432300:CDS
AGTCAAACGCCCCACAAATT+TGG 0.272458 3_2:+84482760 Msa0432300:CDS
TGATTTGGTAGTCAGATTAA+AGG 0.286733 3_2:+84482732 Msa0432300:CDS
TTCTGGCGGAGTTTCAATAT+TGG 0.300547 3_2:-84483059 None:intergenic
TGATAACCCGATTCCACTTT+GGG 0.338959 3_2:+84482798 Msa0432300:CDS
CACCAAGGTTGTGGAGCGTT+TGG 0.362879 3_2:+84482947 Msa0432300:CDS
CAACACTTCGGGTGCTTTGA+AGG 0.372651 3_2:-84482831 None:intergenic
TTAACTATTACTCGTGAGAT+AGG 0.379484 3_2:+84483227 Msa0432300:CDS
GGTAGTTGTTCAATATCTTC+TGG 0.404192 3_2:-84483076 None:intergenic
AAATTTACATGCATTCTTGA+CGG 0.436049 3_2:-84483168 None:intergenic
TATAGGATAACCAAATTTGT+GGG 0.436442 3_2:-84482770 None:intergenic
GTGTAAGCCCAAAGTGGAAT+CGG 0.438507 3_2:-84482805 None:intergenic
GTGATAACCCGATTCCACTT+TGG 0.443095 3_2:+84482797 Msa0432300:CDS
GCATAGAGAAGGTGCGTTGA+AGG 0.466723 3_2:+84482878 Msa0432300:intron
TGTAAATCCGACTGGGATGC+AGG 0.468237 3_2:+84482905 Msa0432300:CDS
TTATAGGATAACCAAATTTG+TGG 0.487958 3_2:-84482771 None:intergenic
TGTTACTTATCACGTTTCTG+AGG 0.497944 3_2:-84483265 None:intergenic
TTATAGAATCACCAAGGTTG+TGG 0.498080 3_2:+84482938 Msa0432300:CDS
TGTAAGCCCAAAGTGGAATC+GGG 0.505125 3_2:-84482804 None:intergenic
AAGGGAAATGTAAATCCGAC+TGG 0.506040 3_2:+84482897 Msa0432300:CDS
AACACTTCGGGTGCTTTGAA+GGG 0.535051 3_2:-84482830 None:intergenic
TAGTACTTGTTATTCTGCAA+TGG 0.537206 3_2:+84483292 Msa0432300:CDS
CATAGAGAAGGTGCGTTGAA+GGG 0.537585 3_2:+84482879 Msa0432300:intron
AGAATGCATGTAAATTTGGA+TGG 0.539085 3_2:+84483174 Msa0432300:CDS
CTATGGCTCTCCGTCTCCTG+AGG 0.555426 3_2:+84483019 Msa0432300:CDS
AGCCAAACGCTCCACAACCT+TGG 0.564815 3_2:-84482949 None:intergenic
GTTACTTATCACGTTTCTGA+GGG 0.572268 3_2:-84483264 None:intergenic
AGGTTGTGGAGCGTTTGGCT+AGG 0.588926 3_2:+84482952 Msa0432300:CDS
TATGGCTCTCCGTCTCCTGA+GGG 0.597298 3_2:+84483020 Msa0432300:CDS
ATAATCAATCCCTCAGGAGA+CGG 0.598701 3_2:-84483029 None:intergenic
AAGTGCATAATCAATCCCTC+AGG 0.601496 3_2:-84483035 None:intergenic
TTTGGTAGTCAGATTAAAGG+TGG 0.606380 3_2:+84482666 None:intergenic
ATTGCAGAATAACAAGTACT+AGG 0.614605 3_2:-84483290 None:intergenic
ATAGGATAACCAAATTTGTG+GGG 0.631303 3_2:-84482769 None:intergenic
TGCATATTCTTCGACAACAT+AGG 0.650468 3_2:-84483097 None:intergenic
TGATGAATTAGCAAGTGTGA+TGG 0.651180 3_2:+84483133 Msa0432300:CDS
AGGGAAGTGTAAGCCCAAAG+TGG 0.655941 3_2:-84482811 None:intergenic
TTGATGACCTGCATCCCAGT+CGG 0.656366 3_2:-84482912 None:intergenic
AGTTGTTCAATATCTTCTGG+CGG 0.661594 3_2:-84483073 None:intergenic
AGGGAAATGTAAATCCGACT+GGG 0.662637 3_2:+84482898 Msa0432300:CDS
CAACTATTATAGAATCACCA+AGG 0.712891 3_2:+84482932 Msa0432300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCAAATTTTTTAAGCTA+TGG + chr3_2:84483002-84483021 Msa0432300:CDS 15.0%
!! AAATTTGGTTATCCTATAAA+AGG + chr3_2:84482775-84482794 Msa0432300:CDS 20.0%
!!! GAAAATTCGATTTTATACTT+GGG - chr3_2:84482987-84483006 None:intergenic 20.0%
!!! TGAAAATTCGATTTTATACT+TGG - chr3_2:84482988-84483007 None:intergenic 20.0%
! TATAGGATAACCAAATTTGT+GGG - chr3_2:84482773-84482792 None:intergenic 25.0%
! TTATAGGATAACCAAATTTG+TGG - chr3_2:84482774-84482793 None:intergenic 25.0%
! AAATTTACATGCATTCTTGA+CGG - chr3_2:84483171-84483190 None:intergenic 25.0%
!!! CTTTTGACTCTAATTTGTTA+AGG - chr3_2:84483202-84483221 None:intergenic 25.0%
! AAAAATCGACAGAGTTGATT+TGG + chr3_2:84482717-84482736 Msa0432300:CDS 30.0%
!! TGATTTGGTAGTCAGATTAA+AGG + chr3_2:84482732-84482751 Msa0432300:CDS 30.0%
ATAGGATAACCAAATTTGTG+GGG - chr3_2:84482772-84482791 None:intergenic 30.0%
CAACTATTATAGAATCACCA+AGG + chr3_2:84482932-84482951 Msa0432300:CDS 30.0%
GTCAAGAATGCATGTAAATT+TGG + chr3_2:84483170-84483189 Msa0432300:CDS 30.0%
AGAATGCATGTAAATTTGGA+TGG + chr3_2:84483174-84483193 Msa0432300:CDS 30.0%
TTAACTATTACTCGTGAGAT+AGG + chr3_2:84483227-84483246 Msa0432300:CDS 30.0%
ATTGCAGAATAACAAGTACT+AGG - chr3_2:84483293-84483312 None:intergenic 30.0%
!! TAGTACTTGTTATTCTGCAA+TGG + chr3_2:84483292-84483311 Msa0432300:CDS 30.0%
! ATCGGGTTATCACCTTTTAT+AGG - chr3_2:84482790-84482809 None:intergenic 35.0%
TTGGATTCTATAGCGGTTTA+TGG + chr3_2:84482851-84482870 Msa0432300:intron 35.0%
TTATAGAATCACCAAGGTTG+TGG + chr3_2:84482938-84482957 Msa0432300:CDS 35.0%
!!! CGATTTTATACTTGGGTTGA+AGG - chr3_2:84482980-84482999 None:intergenic 35.0%
AGTTGTTCAATATCTTCTGG+CGG - chr3_2:84483076-84483095 None:intergenic 35.0%
GGTAGTTGTTCAATATCTTC+TGG - chr3_2:84483079-84483098 None:intergenic 35.0%
TGCATATTCTTCGACAACAT+AGG - chr3_2:84483100-84483119 None:intergenic 35.0%
TGATGAATTAGCAAGTGTGA+TGG + chr3_2:84483133-84483152 Msa0432300:CDS 35.0%
GTTACTTATCACGTTTCTGA+GGG - chr3_2:84483267-84483286 None:intergenic 35.0%
! TGTTACTTATCACGTTTCTG+AGG - chr3_2:84483268-84483287 None:intergenic 35.0%
TGATAACCCGATTCCACTTT+GGG + chr3_2:84482798-84482817 Msa0432300:CDS 40.0%
GCTATAGAATCCAACACTTC+GGG - chr3_2:84482845-84482864 None:intergenic 40.0%
CGCTATAGAATCCAACACTT+CGG - chr3_2:84482846-84482865 None:intergenic 40.0%
!! CGAAGTGTTGGATTCTATAG+CGG + chr3_2:84482844-84482863 Msa0432300:intron 40.0%
TTTATGGCACTGCATAGAGA+AGG + chr3_2:84482867-84482886 Msa0432300:intron 40.0%
AAGGGAAATGTAAATCCGAC+TGG + chr3_2:84482897-84482916 Msa0432300:CDS 40.0%
AGGGAAATGTAAATCCGACT+GGG + chr3_2:84482898-84482917 Msa0432300:CDS 40.0%
ATAATCAATCCCTCAGGAGA+CGG - chr3_2:84483032-84483051 None:intergenic 40.0%
AAGTGCATAATCAATCCCTC+AGG - chr3_2:84483038-84483057 None:intergenic 40.0%
TTCTGGCGGAGTTTCAATAT+TGG - chr3_2:84483062-84483081 None:intergenic 40.0%
! CGATTTTTATGCTCGTCTCG+AGG - chr3_2:84482705-84482724 None:intergenic 45.0%
AGTCAAACGCCCCACAAATT+TGG + chr3_2:84482760-84482779 Msa0432300:CDS 45.0%
GTGATAACCCGATTCCACTT+TGG + chr3_2:84482797-84482816 Msa0432300:CDS 45.0%
TGTAAGCCCAAAGTGGAATC+GGG - chr3_2:84482807-84482826 None:intergenic 45.0%
GTGTAAGCCCAAAGTGGAAT+CGG - chr3_2:84482808-84482827 None:intergenic 45.0%
! AACACTTCGGGTGCTTTGAA+GGG - chr3_2:84482833-84482852 None:intergenic 45.0%
! CATAGAGAAGGTGCGTTGAA+GGG + chr3_2:84482879-84482898 Msa0432300:intron 45.0%
! AGGGAAGTGTAAGCCCAAAG+TGG - chr3_2:84482814-84482833 None:intergenic 50.0%
CAACACTTCGGGTGCTTTGA+AGG - chr3_2:84482834-84482853 None:intergenic 50.0%
! CTTCAAAGCACCCGAAGTGT+TGG + chr3_2:84482832-84482851 Msa0432300:intron 50.0%
! GCATAGAGAAGGTGCGTTGA+AGG + chr3_2:84482878-84482897 Msa0432300:intron 50.0%
TGTAAATCCGACTGGGATGC+AGG + chr3_2:84482905-84482924 Msa0432300:CDS 50.0%
! TTGATGACCTGCATCCCAGT+CGG - chr3_2:84482915-84482934 None:intergenic 50.0%
! CACCAAGGTTGTGGAGCGTT+TGG + chr3_2:84482947-84482966 Msa0432300:CDS 55.0%
AGCCAAACGCTCCACAACCT+TGG - chr3_2:84482952-84482971 None:intergenic 55.0%
! AGGTTGTGGAGCGTTTGGCT+AGG + chr3_2:84482952-84482971 Msa0432300:CDS 55.0%
TATGGCTCTCCGTCTCCTGA+GGG + chr3_2:84483020-84483039 Msa0432300:CDS 55.0%
CTATGGCTCTCCGTCTCCTG+AGG + chr3_2:84483019-84483038 Msa0432300:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 84482679 84483352 84482679 ID=Msa0432300;Name=Msa0432300
chr3_2 mRNA 84482679 84483352 84482679 ID=Msa0432300-mRNA-1;Parent=Msa0432300;Name=Msa0432300-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|0|0|2|0|211
chr3_2 exon 84482679 84482847 84482679 ID=Msa0432300-mRNA-1:exon:23893;Parent=Msa0432300-mRNA-1
chr3_2 exon 84482889 84483352 84482889 ID=Msa0432300-mRNA-1:exon:23894;Parent=Msa0432300-mRNA-1
chr3_2 CDS 84482679 84482847 84482679 ID=Msa0432300-mRNA-1:cds;Parent=Msa0432300-mRNA-1
chr3_2 CDS 84482889 84483352 84482889 ID=Msa0432300-mRNA-1:cds;Parent=Msa0432300-mRNA-1
Gene Sequence

>Msa0432300

TTAAAGGTGGCAAGTCAAATCATCCTCGAGACGAGCATAAAAATCGACAGAGTTGATTTGGTAGTCAGATTAAAGGTGTCAAGTCAAACGCCCCACAAATTTGGTTATCCTATAAAAGGTGATAACCCGATTCCACTTTGGGCTTACACTTCCCTTCAAAGCACCCGAAGTGCGTTGAAGGGAAATGTAAATCCGACTGGGATGCAGGTCATCAACTATTATAGAATCACCAAGGTTGTGGAGCGTTTGGCTAGGCACCTTCAACCCAAGTATAAAATCGAATTTTCAAATTTTTTAAGCTATGGCTCTCCGTCTCCTGAGGGATTGATTATGCACTTGCCAATATTGAAACTCCGCCAGAAGATATTGAACAACTACCTATGTTGTCGAAGAATATGCATTATCAAAGAAAGTGATGAATTAGCAAGTGTGATGGTTTTAATGATATCCGTCAAGAATGCATGTAAATTTGGATGGTTCCTTAACAAATTAGAGTCAAAAGAACTTTTAACTATTACTCGTGAGATAGGAAAGATCTATTGTACCCTCAGAAACGTGATAAGTAACAGACCTAGTACTTGTTATTCTGCAATGGTATCAACTATAACAGAAAGTTTCTATCCAAATATGAAT

Protein sequence

>Msa0432300

LKVASQIILETSIKIDRVDLVVRLKVSSQTPHKFGYPIKGDNPIPLWAYTSLQSTRSALKGNVNPTGMQVINYYRITKVVERLARHLQPKYKIEFSNFLSYGSPSPEGLIMHLPILKLRQKILNNYLCCRRICIIKESDELASVMVLMISVKNACKFGWFLNKLESKELLTITREIGKIYCTLRNVISNRPSTCYSAMVSTITESFYPNMN