AlfalfaGEDB | Msa1297210

M. sativa cultivar zhongmu-4 / Msa1297210

KEGG: -
GO: GO:0000003 GO:0000271 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005975 GO:0005976 GO:0006080 GO:0007275 GO:0008150 GO:0008152 GO:0008378 GO:0009058 GO:0009059 GO:0009791 GO:0009987 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010392 GO:0012505 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0022414 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051069 GO:0051070 GO:0061458 GO:0071704 GO:0097708 GO:0098791 GO:1901576
PFAM: PF05637.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297210	sp\|Q564G7\|GMGT1_CYATE	75.000	412	99	3	3	412	2	411	0.0	678

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297210	A0A396IE49	57.754	374	148	3	71	436	56	427	1.75e-148	456
Msa1297210	A0A396IE49	95.631	412	18	0	1	412	448	859	0.0	844

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015400	Msa1297210	0.811114	8.230698e-51	-8.615850e-47
Msa0056530	Msa1297210	0.806142	9.496275e-50	-8.615850e-47
Msa0062860	Msa1297210	0.805789	1.126554e-49	-8.615850e-47
Msa0102070	Msa1297210	0.811978	5.342201e-51	-8.615850e-47
Msa0107580	Msa1297210	0.801299	9.614272e-49	-8.615850e-47
Msa0111510	Msa1297210	0.833485	5.191752e-56	-8.615850e-47
Msa0123810	Msa1297210	0.812238	4.687395e-51	-8.615850e-47
Msa0142220	Msa1297210	0.842912	1.934679e-58	-8.615850e-47
Msa0145750	Msa1297210	0.819896	9.127130e-53	-8.615850e-47
Msa0153910	Msa1297210	0.855240	7.206424e-62	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297210	MtrunA17_Chr4g0054201	95.631	412	18	0	1	412	448	859	0.0	844
Msa1297210	MtrunA17_Chr4g0054201	57.754	374	148	3	71	436	56	427	3.38e-152	456
Msa1297210	MtrunA17_Chr5g0401011	64.634	410	132	3	8	412	21	422	0.0	569
Msa1297210	MtrunA17_Chr4g0061061	47.024	336	142	5	80	412	101	403	1.66e-107	327
Msa1297210	MtrunA17_Chr3g0091621	46.087	345	146	6	75	412	97	408	3.97e-105	321
Msa1297210	MtrunA17_Chr4g0067341	43.976	332	152	4	84	412	107	407	4.09e-102	313
Msa1297210	MtrunA17_Chr7g0269221	43.425	327	151	4	89	412	124	419	2.05e-98	305

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1297210	AT2G22900.1	55.156	417	172	3	1	412	16	422	3.47e-170	488
Msa1297210	AT4G37690.1	54.930	426	174	5	1	421	1	413	4.06e-168	482
Msa1297210	AT5G07720.1	46.491	342	146	5	74	412	105	412	2.47e-108	330
Msa1297210	AT1G18690.3	42.588	371	170	5	54	412	132	471	7.26e-107	328
Msa1297210	AT1G18690.4	42.588	371	170	5	54	412	132	471	7.26e-107	328
Msa1297210	AT1G18690.2	42.588	371	170	5	54	412	132	471	7.26e-107	328
Msa1297210	AT1G18690.1	42.588	371	170	5	54	412	132	471	7.26e-107	328
Msa1297210	AT1G74380.1	45.217	345	149	4	77	412	100	413	1.23e-106	326
Msa1297210	AT4G02500.1	41.828	361	164	5	84	429	113	442	3.72e-99	306
Msa1297210	AT3G62720.2	43.731	327	150	4	89	412	117	412	1.51e-97	302

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Find 114 sgRNAs with CRISPR-Local

Find 357 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGTTAGCAAGGATGTGT+TGG	0.480699	8_3:+57154233	Msa1297210:CDS
AACATCGCAGCCCTAACCAT+AGG	0.646911	8_3:-57149202	None:intergenic
AAGAAAGAACATAGCTGGAC+TGG	0.523232	8_3:+57149355	Msa1297210:CDS
AATGTTAGCAAGGATGTGTT+GGG	0.487842	8_3:+57154234	Msa1297210:CDS
ACAAAGTGTGATAACCCGAT+TGG	0.602085	8_3:+57149058	Msa1297210:CDS
ACAACCTTGTGATTCATGGT+TGG	0.510440	8_3:+57149320	Msa1297210:CDS
ACAGGGTGCCAACCTTGTAA+TGG	0.477110	8_3:+57149802	Msa1297210:CDS
ACAGTCCAGAATACGAGAAA+TGG	0.392275	8_3:+57149458	Msa1297210:CDS
ACCACAAACACCGACTTTGC+TGG	0.348992	8_3:+57148857	Msa1297210:CDS
ACCAGCAAAGTCGGTGTTTG+TGG	0.459691	8_3:-57148858	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAAAAACAAATCATCTTCA+TGG	+	chr8_3:57148693-57148712	Msa1297210:CDS	30.0%
CATTGTTGTAGATTATGTCA+TGG	-	chr8_3:57149141-57149160	None:intergenic	30.0%
ATTGGACTTTATGGATGTTT+GGG	+	chr8_3:57149420-57149439	Msa1297210:CDS	30.0%
AGATAGAAAAAAGGGTTGAA+GGG	+	chr8_3:57149674-57149693	Msa1297210:CDS	30.0%
ACAGACTTATCCAATAGATT+TGG	-	chr8_3:57149931-57149950	None:intergenic	30.0%
TAGATGATTTCATTGGATCA+TGG	+	chr8_3:57150086-57150105	Msa1297210:intron	30.0%
TTATTCTACACCATATCTCT+TGG	+	chr8_3:57150111-57150130	Msa1297210:intron	30.0%
GCAAGAAATAGGCTTATATA+TGG	+	chr8_3:57150210-57150229	Msa1297210:intron	30.0%
TTTGAACCAACACAATTTCA+AGG	+	chr8_3:57150276-57150295	Msa1297210:intron	30.0%
TTCATACCTTGAAATTGTGT+TGG	-	chr8_3:57150285-57150304	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	57148674	57154287	57148674	ID=Msa1297210;Name=Msa1297210
chr8_3	mRNA	57148674	57154287	57148674	ID=Msa1297210-mRNA-1;Parent=Msa1297210;Name=Msa1297210-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0\|0\|0.2\|1\|1\|5\|0\|501
chr8_3	exon	57148674	57149900	57148674	ID=Msa1297210-mRNA-1:exon:16398;Parent=Msa1297210-mRNA-1
chr8_3	exon	57153239	57153321	57153239	ID=Msa1297210-mRNA-1:exon:16399;Parent=Msa1297210-mRNA-1
chr8_3	exon	57153872	57153904	57153872	ID=Msa1297210-mRNA-1:exon:16400;Parent=Msa1297210-mRNA-1
chr8_3	exon	57154010	57154076	57154010	ID=Msa1297210-mRNA-1:exon:16401;Parent=Msa1297210-mRNA-1
chr8_3	exon	57154192	57154287	57154192	ID=Msa1297210-mRNA-1:exon:16402;Parent=Msa1297210-mRNA-1
chr8_3	CDS	57148674	57149900	57148674	ID=Msa1297210-mRNA-1:cds;Parent=Msa1297210-mRNA-1
chr8_3	CDS	57153239	57153321	57153239	ID=Msa1297210-mRNA-1:cds;Parent=Msa1297210-mRNA-1
chr8_3	CDS	57153872	57153904	57153872	ID=Msa1297210-mRNA-1:cds;Parent=Msa1297210-mRNA-1
chr8_3	CDS	57154010	57154076	57154010	ID=Msa1297210-mRNA-1:cds;Parent=Msa1297210-mRNA-1
chr8_3	CDS	57154192	57154287	57154192	ID=Msa1297210-mRNA-1:cds;Parent=Msa1297210-mRNA-1

Gene Sequence

>Msa1297210

ATGGCAACCAAATTTGGTTCCAAAAACAAATCATCTTCATGGCTCTCAAGTGGTTGCATCTTCCTCTTAGGTGCAATGTCAGCTCTTCTTTTTATCTGGGGCCTCTCCTCCTTCATCACTCCTATCCCAAACACCAACACCAAATTCAACTCCATCACCACTAAACTCAAATCCTTTAACTTCACCACAAACACCGACTTTGCTGGTCCTGATTTGTTACACGACCCTTCAGACAAAACCTTCTATGATGATCCACAAACATGTTACACCATGGACAAACCGATGAAAAATTGGGACGAGAAGCGTCAAGAATGGCTACTTCATCATCCTTCGTTCGTGGCTGGAGCAAGCGAAAAGATACTTGTTATAACGGGTTCACAACCTACAAAGTGTGATAACCCGATTGGCGACCACCTTTTACTGCGGTTCTTTAAAAACAAGGTTGATTATTGTCGTATACACAACCATGACATAATCTACAACAATGCATTGTTACACCCAAAAATGGACTCTTATTGGGCCAAGTATCCTATGGTTAGGGCTGCGATGTTGGCTCATCCGGAAGTTGAATGGATATGGTGGGTCGACTCTGATGCCATCTTCACCGACATGGAGTTCAAGTTACCGTTATGGCGTTACAATGATCACAACCTTGTGATTCATGGTTGGGAAGAGTTGGTAAAGAAAGAACATAGCTGGACTGGCCTAAACGCGGGTGTTTTCTTGATGAGGAATTGTCAGTGGTCATTGGACTTTATGGATGTTTGGGCCAGTATGGGCCCAAACAGTCCAGAATACGAGAAATGGGGGGAGAGGCTAAGAGCAACTTTCAAGACAAAAGTGGTACCTGATTCAGATGATCAGACATCGCTTGCTTACTTGATCGCGATGGGAGAGGACAAGTGGACAAAAAAGATCTACATGGAGAATGAGTATTATTTTGAAGGGTATTGGTTGGAGCTTTCAAAGATGTATGATAAAATGGGTGAGAAATATGATGAGATAGAAAAAAGGGTTGAAGGGTTAAGGAGAAGGCATGCAGAGAAAGTGAGTGAACGTTATGGTGAGATGAGGGAGGAATATGTTAAGAATTTAGGTGACTTGAGAAGACCTTTTATTACACATTTTACAGGGTGCCAACCTTGTAATGGTCACCATAATCCAATGTACGCTGCAGATGATTGCTGGAATGGCATGGAGAGAGCTCTCAATTTTGCTGATAATCAGACTTTGCGTGATGTGAAACGCTATCAAGATTCTGACTCTGAGTCTGATGAATCGCAAGAGTATTACGCTGGTGGTCAGAAGAGGCCTTCAATTGGATACGATGGCACAATGATCAAATTCAATAATACTTTGCATTGTTTTTCACAAATATCAATGTTAGAAGTGTATGGTTGTAGGTTGCTGGAAAATGTAAAAATTAAAGAAGAAATGGGTTTCCCCTTAGAAAATGTTAGCAAGGATGTGTTGGGTTCTACTATTGAGGTTTGTGTTATACTGTGA

Protein sequence

>Msa1297210

MATKFGSKNKSSSWLSSGCIFLLGAMSALLFIWGLSSFITPIPNTNTKFNSITTKLKSFNFTTNTDFAGPDLLHDPSDKTFYDDPQTCYTMDKPMKNWDEKRQEWLLHHPSFVAGASEKILVITGSQPTKCDNPIGDHLLLRFFKNKVDYCRIHNHDIIYNNALLHPKMDSYWAKYPMVRAAMLAHPEVEWIWWVDSDAIFTDMEFKLPLWRYNDHNLVIHGWEELVKKEHSWTGLNAGVFLMRNCQWSLDFMDVWASMGPNSPEYEKWGERLRATFKTKVVPDSDDQTSLAYLIAMGEDKWTKKIYMENEYYFEGYWLELSKMYDKMGEKYDEIEKRVEGLRRRHAEKVSERYGEMREEYVKNLGDLRRPFITHFTGCQPCNGHHNPMYAADDCWNGMERALNFADNQTLRDVKRYQDSDSESDESQEYYAGGQKRPSIGYDGTMIKFNNTLHCFSQISMLEVYGCRLLENVKIKEEMGFPLENVSKDVLGSTIEVCVIL*