AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1101070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1101070 MtrunA17_Chr7g0252721 93.133 233 10 2 1 227 1 233 5.78e-161 444
Msa1101070 MtrunA17_Chr1g0213561 41.379 203 103 3 18 217 14 203 1.59e-43 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1101070 AT3G53860.1 45.872 218 109 2 8 218 52 267 1.54e-62 197
Msa1101070 AT2G31600.3 44.444 234 105 5 8 218 56 287 2.46e-62 197
Msa1101070 AT2G31600.1 44.444 234 105 5 8 218 56 287 2.46e-62 197
Msa1101070 AT2G31600.4 44.444 234 105 5 8 218 56 287 2.46e-62 197
Msa1101070 AT2G31600.5 44.444 234 105 5 8 218 56 287 2.46e-62 197
Msa1101070 AT1G05860.1 45.148 237 109 4 1 218 32 266 1.18e-58 187
Msa1101070 AT1G05860.2 45.148 237 109 4 1 218 32 266 1.18e-58 187
Msa1101070 AT1G05860.3 46.341 164 67 4 1 145 32 193 3.36e-36 127
Msa1101070 AT2G31600.2 43.750 160 65 5 8 144 56 213 2.15e-35 125
Msa1101070 AT2G31600.6 43.750 160 65 5 8 144 56 213 2.15e-35 125

Find 90 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGCTTCGTGCTGTAAAA+AGG 0.204169 7_3:-31608972 Msa1101070:CDS
AAGATAATGATGCTGATTAT+TGG 0.208253 7_3:-31608811 Msa1101070:three_prime_UTR
ATACAGAGTCGCAAAATTTA+AGG 0.217918 7_3:+31608628 None:intergenic
TAACTCTGTTTCTCGGATAT+TGG 0.233652 7_3:+31608736 None:intergenic
TGAATTTGGCGGACAAATTC+AGG 0.235095 7_3:+31608892 None:intergenic
CATAATAACAATTTGTTGAT+TGG 0.244399 7_3:-31608537 Msa1101070:three_prime_UTR
GCTGTTACCGAGGTCATTAC+TGG 0.271359 7_3:-31610989 Msa1101070:CDS
AACGCTTCTGCTGCTGCTGC+TGG 0.277778 7_3:-31610873 Msa1101070:CDS
AACAATTTGTTGATTGGATC+TGG 0.288901 7_3:-31608531 Msa1101070:three_prime_UTR
ATAATCAGCATCATTATCTT+TGG 0.295406 7_3:+31608814 None:intergenic
AGGTCATTACTGGGCGTTAA+TGG 0.298106 7_3:-31610979 Msa1101070:CDS
GGTTAATCAGTCTCAGGTTT+AGG 0.308564 7_3:+31608835 None:intergenic
GGACTAGGCTTACCATTAAC+TGG 0.312740 7_3:+31608934 None:intergenic
AAAGACATTGCACAGTATAT+AGG 0.325419 7_3:-31608770 Msa1101070:three_prime_UTR
GCTTTATGCAGTTTGCCAAC+AGG 0.332413 7_3:-31609075 Msa1101070:intron
GGTCATAACGTCCCAGTTAA+TGG 0.342126 7_3:-31608946 Msa1101070:CDS
GGAAGATGAAGAAAGAAATT+CGG 0.342169 7_3:-31610898 Msa1101070:CDS
CTAAGTACAGAGAGTATTAC+TGG 0.348566 7_3:-31610944 Msa1101070:CDS
GTTGCACCGCCGTGGCAAAA+AGG 0.349754 7_3:-31610725 Msa1101070:intron
CGAGAAACAGAGTTATGATT+TGG 0.355665 7_3:-31608728 Msa1101070:three_prime_UTR
CTAGCATACTCACAATTTAC+AGG 0.364573 7_3:+31608657 None:intergenic
AGAGAGTATTACTGGACTTA+TGG 0.365244 7_3:-31610936 Msa1101070:CDS
GCTGCTGGTGCTGTTGATAA+TGG 0.373434 7_3:-31610858 Msa1101070:CDS
GGATTGGTTGGAGAGATTTA+CGG 0.383007 7_3:+31611170 None:intergenic
ATTGAGATCCATGGTTCCTT+TGG 0.385033 7_3:-31609028 Msa1101070:CDS
GAAGATGAAGAAAGAAATTC+GGG 0.400485 7_3:-31610897 Msa1101070:CDS
AAGACATTGCACAGTATATA+GGG 0.406322 7_3:-31608769 Msa1101070:three_prime_UTR
ATCTTTGGTTAATCAGTCTC+AGG 0.414837 7_3:+31608829 None:intergenic
CCACGATAAAGCTTCTGATT+AGG 0.415729 7_3:+31610747 None:intergenic
GCCAATAAAATGACCGGGTA+AGG 0.421804 7_3:+31608605 None:intergenic
CACGGCGGAGGCGGCGGACG+AGG 0.423786 7_3:+31611027 None:intergenic
TTCCATTGGTGGTGGTGGAT+TGG 0.426391 7_3:+31611154 None:intergenic
ATTGGTGGTGGTGGATTGGT+TGG 0.433418 7_3:+31611158 None:intergenic
GTCTGACACGGCGGAGGCGG+CGG 0.433953 7_3:+31611021 None:intergenic
GAGTCTTCCATTGGTGGTGG+TGG 0.438608 7_3:+31611149 None:intergenic
AATAGATGTAACTGCGGACT+AGG 0.448722 7_3:+31608919 None:intergenic
GACCTCGCGGCGGGTTAAAA+CGG 0.448768 7_3:+31611049 None:intergenic
TCGCCTAATTGGTAATGAGC+AGG 0.449043 7_3:+31609000 None:intergenic
CTTAATAAGAGCCATGGCCT+TGG 0.454027 7_3:+31610797 None:intergenic
GGAACCATGGATCTCAATAC+TGG 0.454340 7_3:+31609033 None:intergenic
TGCCCTGCTCATTACCAATT+AGG 0.459872 7_3:-31609003 Msa1101070:CDS
CTTTCTTCATCTTCCTTGAA+CGG 0.468778 7_3:+31610906 None:intergenic
GTTGTCTGACACGGCGGAGG+CGG 0.468951 7_3:+31611018 None:intergenic
CAAATCATAACTCTGTTTCT+CGG 0.478717 7_3:+31608729 None:intergenic
TACAGAGTCGCAAAATTTAA+GGG 0.480691 7_3:+31608629 None:intergenic
TAGAATGCCAATAAAATGAC+CGG 0.482423 7_3:+31608599 None:intergenic
GGCGTTAATGGAAGAGCTTA+AGG 0.485899 7_3:-31610967 Msa1101070:CDS
TAAACGAAAAGCTGCGCGCA+AGG 0.489819 7_3:-31608866 Msa1101070:CDS
GCGGACGAGGACCTCGCGGC+GGG 0.498422 7_3:+31611040 None:intergenic
CGCCTAATTGGTAATGAGCA+GGG 0.500265 7_3:+31609001 None:intergenic
CTTTATGCAGTTTGCCAACA+GGG 0.500980 7_3:-31609074 Msa1101070:intron
TGTTGTTGAGAGTCTTCCAT+TGG 0.503422 7_3:+31611140 None:intergenic
TTATGGAAGGAGTCCGTTCA+AGG 0.519406 7_3:-31610919 Msa1101070:CDS
AACCAATCCACCACCACCAA+TGG 0.523947 7_3:-31611156 Msa1101070:exon
TGCGACTCTGTATCCTTACC+CGG 0.530731 7_3:-31608618 Msa1101070:three_prime_UTR
TGAGAGTCTTCCATTGGTGG+TGG 0.545791 7_3:+31611146 None:intergenic
GCACAGTATATAGGGAGGGC+AGG 0.552643 7_3:-31608761 Msa1101070:three_prime_UTR
TGTTGAGAGTCTTCCATTGG+TGG 0.554191 7_3:+31611143 None:intergenic
GTCATCGGCGCGATTTGTTG+CGG 0.555912 7_3:+31611113 None:intergenic
ACTGGCTTATTACAAAAGAC+TGG 0.557434 7_3:+31609051 None:intergenic
TCAGCACCGTCGATTGTCAT+CGG 0.558776 7_3:+31611098 None:intergenic
GACTAGGCTTACCATTAACT+GGG 0.570203 7_3:+31608935 None:intergenic
TTACAAAAGACTGGCCCTGT+TGG 0.574396 7_3:+31609060 None:intergenic
CTGTTACCGAGGTCATTACT+GGG 0.578965 7_3:-31610988 Msa1101070:CDS
AGAGTTGTCTGACACGGCGG+AGG 0.587249 7_3:+31611015 None:intergenic
CAACTCTCTCGCTGTTACCG+AGG 0.590857 7_3:-31610999 Msa1101070:CDS
ACAATACTTAATAAGAGCCA+TGG 0.602808 7_3:+31610791 None:intergenic
AGTATTACTGGACTTATGGA+AGG 0.608751 7_3:-31610932 Msa1101070:CDS
GGCGGACGAGGACCTCGCGG+CGG 0.609662 7_3:+31611039 None:intergenic
TTTGTTCGGTGTGCTTCTAG+TGG 0.612439 7_3:-31610828 Msa1101070:CDS
TAAGCCAGTATTGAGATCCA+TGG 0.612741 7_3:-31609037 Msa1101070:CDS
TTATCGTGGTTGCACCGCCG+TGG 0.625527 7_3:-31610733 Msa1101070:CDS
ATCGGCGCGATTTGTTGCGG+TGG 0.628254 7_3:+31611116 None:intergenic
GGCGGCGGACGAGGACCTCG+CGG 0.628435 7_3:+31611036 None:intergenic
TGGTTGCAAGACCAAGGCCA+TGG 0.628995 7_3:-31610808 Msa1101070:CDS
TTCTAGTGGTTGCAAGACCA+AGG 0.629369 7_3:-31610814 Msa1101070:CDS
TAATGAGCAGGGCATACCAA+AGG 0.651701 7_3:+31609012 None:intergenic
AGAATGCCAATAAAATGACC+GGG 0.656066 7_3:+31608600 None:intergenic
CATTGCACAGTATATAGGGA+GGG 0.657863 7_3:-31608765 Msa1101070:three_prime_UTR
CTCGCGGCGGGTTAAAACGG+AGG 0.662601 7_3:+31611052 None:intergenic
ATCGCGCCGATGACAATCGA+CGG 0.665199 7_3:-31611104 Msa1101070:CDS
CCTAATCAGAAGCTTTATCG+TGG 0.666724 7_3:-31610747 Msa1101070:CDS
TTAAAACGGAGGAGTTAGCG+AGG 0.671046 7_3:+31611063 None:intergenic
AGGGCATACCAAAGGAACCA+TGG 0.672546 7_3:+31609020 None:intergenic
AGCGAGAGAGTTGTCTGACA+CGG 0.684863 7_3:+31611009 None:intergenic
ACATTGCACAGTATATAGGG+AGG 0.686301 7_3:-31608766 Msa1101070:three_prime_UTR
TAAAACGGAGGAGTTAGCGA+GGG 0.688548 7_3:+31611064 None:intergenic
GAGAGAGTTGTCTGACACGG+CGG 0.692611 7_3:+31611012 None:intergenic
GGAATTAATAGATGTAACTG+CGG 0.701946 7_3:+31608913 None:intergenic
TTAACGCCCAGTAATGACCT+CGG 0.702952 7_3:+31610982 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGATTAATAACAAATA+TGG - chr7_3:31609141-31609160 Msa1101070:intron 10.0%
!!! AAATATTATTATTTTTTGAC+TGG - chr7_3:31609264-31609283 Msa1101070:intron 10.0%
!!! AATATTATTATTTTTTGACT+GGG - chr7_3:31609265-31609284 Msa1101070:intron 10.0%
!! TTAAATTTAATTAGTAGGAT+AGG + chr7_3:31610491-31610510 None:intergenic 15.0%
!! AAGCATTAAATTTAATTAGT+AGG + chr7_3:31610496-31610515 None:intergenic 15.0%
!!! ATTTGGTAATTTGTGTTTTT+AGG - chr7_3:31609182-31609201 Msa1101070:intron 20.0%
!!! TTTGGTAATTTGTGTTTTTA+GGG - chr7_3:31609183-31609202 Msa1101070:intron 20.0%
!!! GAAGAAGATAATGTTTTTTT+TGG - chr7_3:31609220-31609239 Msa1101070:intron 20.0%
!!! TAACTTTGTTTATGTGTTTT+TGG - chr7_3:31609338-31609357 Msa1101070:intron 20.0%
!!! AAATGTGATCATATATGTTT+TGG - chr7_3:31609455-31609474 Msa1101070:intron 20.0%
!!! TTCAAAATTGTTTTTCCTTT+CGG - chr7_3:31609755-31609774 Msa1101070:intron 20.0%
!!! CTATTTTTTTCTCTTGTTTT+TGG - chr7_3:31609789-31609808 Msa1101070:intron 20.0%
!!! CTTTTCGTTTATTTTGAATT+TGG + chr7_3:31610868-31610887 None:intergenic 20.0%
!!! CATAATAACAATTTGTTGAT+TGG - chr7_3:31611206-31611225 Msa1101070:five_prime_UTR 20.0%
!!! ATAATGGTGATGATTTTGTT+CGG - chr7_3:31608901-31608920 Msa1101070:CDS 25.0%
!!! TACTGTATTGTTTTTGATGA+TGG - chr7_3:31609097-31609116 Msa1101070:intron 25.0%
! TAATTGTGTTTCTTGCAATT+TGG - chr7_3:31609165-31609184 Msa1101070:intron 25.0%
!!! TTTTTTTGGATTTTCTTGTG+TGG - chr7_3:31609234-31609253 Msa1101070:intron 25.0%
!!! ATTATTTTTTGACTGGGTTT+TGG - chr7_3:31609271-31609290 Msa1101070:intron 25.0%
! ACAAACACATATAGATTGAA+TGG - chr7_3:31609843-31609862 Msa1101070:intron 25.0%
! AAAAGGTCAAGGTTATTATT+CGG + chr7_3:31610053-31610072 None:intergenic 25.0%
!! AATAATAACCTTGACCTTTT+TGG - chr7_3:31610053-31610072 Msa1101070:intron 25.0%
! AAAAACTGTATAATGCAAGA+TGG + chr7_3:31610418-31610437 None:intergenic 25.0%
!! ATCTTGCATTATACAGTTTT+TGG - chr7_3:31610417-31610436 Msa1101070:intron 25.0%
!!! ACAGTTTTTGGTTTTTATAC+AGG - chr7_3:31610429-31610448 Msa1101070:intron 25.0%
! GTAATACATGAAAACAGAAA+AGG + chr7_3:31610582-31610601 None:intergenic 25.0%
!!! TTCGTTTATTTTGAATTTGG+CGG + chr7_3:31610865-31610884 None:intergenic 25.0%
! ATAATCAGCATCATTATCTT+TGG + chr7_3:31610932-31610951 None:intergenic 25.0%
!! AAGATAATGATGCTGATTAT+TGG - chr7_3:31610932-31610951 Msa1101070:CDS 25.0%
GGAAGATGAAGAAAGAAATT+CGG - chr7_3:31608845-31608864 Msa1101070:CDS 30.0%
GAAGATGAAGAAAGAAATTC+GGG - chr7_3:31608846-31608865 Msa1101070:CDS 30.0%
ACAATACTTAATAAGAGCCA+TGG + chr7_3:31608955-31608974 None:intergenic 30.0%
!! TTGTGGTTGATGAGAAAAAT+GGG - chr7_3:31609315-31609334 Msa1101070:intron 30.0%
! TGTTGTTGTTCTTGACATAT+TGG - chr7_3:31609494-31609513 Msa1101070:intron 30.0%
ATTGACATAATTCAACGTGA+TGG - chr7_3:31609584-31609603 Msa1101070:intron 30.0%
!!! ATAGTCATGTTTTAACCGAA+AGG + chr7_3:31609773-31609792 None:intergenic 30.0%
ACATAAACACTTGTAGTCTA+TGG - chr7_3:31609817-31609836 Msa1101070:intron 30.0%
! ACATTGTCAATCTGAAATGT+TGG - chr7_3:31609918-31609937 Msa1101070:intron 30.0%
AATTGTAATTCAACAGAGTG+TGG - chr7_3:31609962-31609981 Msa1101070:intron 30.0%
ATTGTAATTCAACAGAGTGT+GGG - chr7_3:31609963-31609982 Msa1101070:intron 30.0%
CATGAACTGAATAAAGATTG+AGG + chr7_3:31610027-31610046 None:intergenic 30.0%
CTCAATCTTTATTCAGTTCA+TGG - chr7_3:31610025-31610044 Msa1101070:intron 30.0%
AAAGGTCAAGGTTATTATTC+GGG + chr7_3:31610052-31610071 None:intergenic 30.0%
CTTGCTTATGATAGAAACTT+GGG - chr7_3:31610136-31610155 Msa1101070:intron 30.0%
GATAGAAACTTGGGTATTAT+AGG - chr7_3:31610145-31610164 Msa1101070:intron 30.0%
CCTTAATACTTACATCTCAA+GGG + chr7_3:31610236-31610255 None:intergenic 30.0%
CCTTGAGATGTAAGTATTAA+GGG - chr7_3:31610234-31610253 Msa1101070:intron 30.0%
! AACAGATATGTATTGATGCT+TGG + chr7_3:31610330-31610349 None:intergenic 30.0%
ATTACCTACAGAAGACAATT+CGG - chr7_3:31610631-31610650 Msa1101070:intron 30.0%
GGAATTAATAGATGTAACTG+CGG + chr7_3:31610833-31610852 None:intergenic 30.0%
AAAGACATTGCACAGTATAT+AGG - chr7_3:31610973-31610992 Msa1101070:CDS 30.0%
AAGACATTGCACAGTATATA+GGG - chr7_3:31610974-31610993 Msa1101070:CDS 30.0%
! CAAATCATAACTCTGTTTCT+CGG + chr7_3:31611017-31611036 None:intergenic 30.0%
TACAGAGTCGCAAAATTTAA+GGG + chr7_3:31611117-31611136 None:intergenic 30.0%
ATACAGAGTCGCAAAATTTA+AGG + chr7_3:31611118-31611137 None:intergenic 30.0%
TAGAATGCCAATAAAATGAC+CGG + chr7_3:31611147-31611166 None:intergenic 30.0%
!! AACAATTTGTTGATTGGATC+TGG - chr7_3:31611212-31611231 Msa1101070:five_prime_UTR 30.0%
! GATTTACGGCGATTCTTTTT+GGG + chr7_3:31608562-31608581 None:intergenic 35.0%
! AGATTTACGGCGATTCTTTT+TGG + chr7_3:31608563-31608582 None:intergenic 35.0%
!! CTAAGTACAGAGAGTATTAC+TGG - chr7_3:31608799-31608818 Msa1101070:three_prime_UTR 35.0%
!! AGAGAGTATTACTGGACTTA+TGG - chr7_3:31608807-31608826 Msa1101070:three_prime_UTR 35.0%
AGTATTACTGGACTTATGGA+AGG - chr7_3:31608811-31608830 Msa1101070:three_prime_UTR 35.0%
CTTTCTTCATCTTCCTTGAA+CGG + chr7_3:31608840-31608859 None:intergenic 35.0%
! AAAAACTCACCTTTTTGCCA+CGG + chr7_3:31609030-31609049 None:intergenic 35.0%
!! GGAATAATGAGTATTGACTG+TGG - chr7_3:31609292-31609311 Msa1101070:intron 35.0%
!! GTTGTGGTTGATGAGAAAAA+TGG - chr7_3:31609314-31609333 Msa1101070:intron 35.0%
CAACTTTCTCAGACTATTAC+AGG + chr7_3:31609428-31609447 None:intergenic 35.0%
ACAACAACACTTCTGATTGA+AGG + chr7_3:31609483-31609502 None:intergenic 35.0%
CATATAGATTGAATGGAACC+AGG - chr7_3:31609850-31609869 Msa1101070:intron 35.0%
CAAGAACTTCGAATCAAACT+TGG + chr7_3:31610079-31610098 None:intergenic 35.0%
AGGATGACAAGATTGAAAGA+AGG + chr7_3:31610118-31610137 None:intergenic 35.0%
CCAAGTTTCTATCATAAGCA+AGG + chr7_3:31610138-31610157 None:intergenic 35.0%
CCTTGCTTATGATAGAAACT+TGG - chr7_3:31610135-31610154 Msa1101070:intron 35.0%
CCCTTGAGATGTAAGTATTA+AGG - chr7_3:31610233-31610252 Msa1101070:intron 35.0%
CCCTTAATACTTACATCTCA+AGG + chr7_3:31610237-31610256 None:intergenic 35.0%
! ATGTATTGATGCTTGGAAGT+GGG + chr7_3:31610323-31610342 None:intergenic 35.0%
! TATGTATTGATGCTTGGAAG+TGG + chr7_3:31610324-31610343 None:intergenic 35.0%
TAGTTGTGTGGCATGTTAAA+AGG - chr7_3:31610360-31610379 Msa1101070:intron 35.0%
AGTTGTGTGGCATGTTAAAA+GGG - chr7_3:31610361-31610380 Msa1101070:intron 35.0%
! CAATTCGGCTGATAAACTTT+TGG - chr7_3:31610646-31610665 Msa1101070:intron 35.0%
! ACTGGCTTATTACAAAAGAC+TGG + chr7_3:31610695-31610714 None:intergenic 35.0%
ATCTTTGGTTAATCAGTCTC+AGG + chr7_3:31610917-31610936 None:intergenic 35.0%
! TAACTCTGTTTCTCGGATAT+TGG + chr7_3:31611010-31611029 None:intergenic 35.0%
CGAGAAACAGAGTTATGATT+TGG - chr7_3:31611015-31611034 Msa1101070:CDS 35.0%
CTAGCATACTCACAATTTAC+AGG + chr7_3:31611089-31611108 None:intergenic 35.0%
AGAATGCCAATAAAATGACC+GGG + chr7_3:31611146-31611165 None:intergenic 35.0%
!! GGATTGGTTGGAGAGATTTA+CGG + chr7_3:31608576-31608595 None:intergenic 40.0%
TGTTGTTGAGAGTCTTCCAT+TGG + chr7_3:31608606-31608625 None:intergenic 40.0%
CCACGATAAAGCTTCTGATT+AGG + chr7_3:31608999-31609018 None:intergenic 40.0%
! CCTAATCAGAAGCTTTATCG+TGG - chr7_3:31608996-31609015 Msa1101070:CDS 40.0%
! TGGATTTTCTTGTGTGGCTT+TGG - chr7_3:31609240-31609259 Msa1101070:intron 40.0%
!! ATGAGTATTGACTGTGGTTG+TGG - chr7_3:31609298-31609317 Msa1101070:intron 40.0%
AATTCAACGTGATGGCATGT+CGG - chr7_3:31609592-31609611 Msa1101070:intron 40.0%
AACACTGGGACATGTCTAAT+CGG - chr7_3:31609637-31609656 Msa1101070:intron 40.0%
CTAACATGTATCAGTGTACC+TGG + chr7_3:31609871-31609890 None:intergenic 40.0%
CACTGATACATGTTAGACAC+CGG - chr7_3:31609875-31609894 Msa1101070:intron 40.0%
TGTGTCTAGTGTCTGAATCA+TGG + chr7_3:31609901-31609920 None:intergenic 40.0%
AAACTTGGCCAAAAAGGTCA+AGG + chr7_3:31610064-31610083 None:intergenic 40.0%
CGAATCAAACTTGGCCAAAA+AGG + chr7_3:31610070-31610089 None:intergenic 40.0%
! GGAGAATTTTCCAAGTGCTT+GGG - chr7_3:31610255-31610274 Msa1101070:intron 40.0%
! GAGAATTTTCCAAGTGCTTG+GGG - chr7_3:31610256-31610275 Msa1101070:intron 40.0%
TTGCCTTCAGTTTAGTTGTG+TGG - chr7_3:31610348-31610367 Msa1101070:intron 40.0%
ATGCCACACAACTAAACTGA+AGG + chr7_3:31610354-31610373 None:intergenic 40.0%
CTTTATGCAGTTTGCCAACA+GGG - chr7_3:31610669-31610688 Msa1101070:intron 40.0%
! TAAGCCAGTATTGAGATCCA+TGG - chr7_3:31610706-31610725 Msa1101070:intron 40.0%
ATTGAGATCCATGGTTCCTT+TGG - chr7_3:31610715-31610734 Msa1101070:intron 40.0%
CTTGCTTCGTGCTGTAAAAA+GGG - chr7_3:31610772-31610791 Msa1101070:CDS 40.0%
GACTAGGCTTACCATTAACT+GGG + chr7_3:31610811-31610830 None:intergenic 40.0%
AATAGATGTAACTGCGGACT+AGG + chr7_3:31610827-31610846 None:intergenic 40.0%
! TGAATTTGGCGGACAAATTC+AGG + chr7_3:31610854-31610873 None:intergenic 40.0%
GGTTAATCAGTCTCAGGTTT+AGG + chr7_3:31610911-31610930 None:intergenic 40.0%
ACATTGCACAGTATATAGGG+AGG - chr7_3:31610977-31610996 Msa1101070:CDS 40.0%
CATTGCACAGTATATAGGGA+GGG - chr7_3:31610978-31610997 Msa1101070:CDS 40.0%
!! TCCTTACCCGGTCATTTTAT+TGG - chr7_3:31611137-31611156 Msa1101070:CDS 40.0%
TGTTGAGAGTCTTCCATTGG+TGG + chr7_3:31608603-31608622 None:intergenic 45.0%
TAAAACGGAGGAGTTAGCGA+GGG + chr7_3:31608682-31608701 None:intergenic 45.0%
TTAAAACGGAGGAGTTAGCG+AGG + chr7_3:31608683-31608702 None:intergenic 45.0%
CTGTTACCGAGGTCATTACT+GGG - chr7_3:31608755-31608774 Msa1101070:three_prime_UTR 45.0%
TTAACGCCCAGTAATGACCT+CGG + chr7_3:31608764-31608783 None:intergenic 45.0%
AGGTCATTACTGGGCGTTAA+TGG - chr7_3:31608764-31608783 Msa1101070:three_prime_UTR 45.0%
GGCGTTAATGGAAGAGCTTA+AGG - chr7_3:31608776-31608795 Msa1101070:three_prime_UTR 45.0%
TTATGGAAGGAGTCCGTTCA+AGG - chr7_3:31608824-31608843 Msa1101070:exon 45.0%
!! TTTGTTCGGTGTGCTTCTAG+TGG - chr7_3:31608915-31608934 Msa1101070:CDS 45.0%
TTCTAGTGGTTGCAAGACCA+AGG - chr7_3:31608929-31608948 Msa1101070:CDS 45.0%
CTTAATAAGAGCCATGGCCT+TGG + chr7_3:31608949-31608968 None:intergenic 45.0%
! TCAGCGTGTCATTGTCATTC+CGG + chr7_3:31609403-31609422 None:intergenic 45.0%
TCTAGTGTCTGAATCATGGC+CGG + chr7_3:31609897-31609916 None:intergenic 45.0%
CATCTCATCCTACTTGACGT+GGG + chr7_3:31610181-31610200 None:intergenic 45.0%
AAGTAGGATGAGATGCTCGT+TGG - chr7_3:31610186-31610205 Msa1101070:intron 45.0%
! GGGAGAATTTTCCAAGTGCT+TGG - chr7_3:31610254-31610273 Msa1101070:intron 45.0%
ATGGCTCATTCACATTGGTG+AGG + chr7_3:31610399-31610418 None:intergenic 45.0%
GCAAGATGGCTCATTCACAT+TGG + chr7_3:31610404-31610423 None:intergenic 45.0%
AGTAGGATAGGTACAGACAG+AGG + chr7_3:31610479-31610498 None:intergenic 45.0%
TCAGCCGAATTGTCTTCTGT+AGG + chr7_3:31610638-31610657 None:intergenic 45.0%
GCTTTATGCAGTTTGCCAAC+AGG - chr7_3:31610668-31610687 Msa1101070:intron 45.0%
! TTACAAAAGACTGGCCCTGT+TGG + chr7_3:31610686-31610705 None:intergenic 45.0%
GGAACCATGGATCTCAATAC+TGG + chr7_3:31610713-31610732 None:intergenic 45.0%
TAATGAGCAGGGCATACCAA+AGG + chr7_3:31610734-31610753 None:intergenic 45.0%
TGCCCTGCTCATTACCAATT+AGG - chr7_3:31610740-31610759 Msa1101070:CDS 45.0%
!! CGCCTAATTGGTAATGAGCA+GGG + chr7_3:31610745-31610764 None:intergenic 45.0%
!! TCGCCTAATTGGTAATGAGC+AGG + chr7_3:31610746-31610765 None:intergenic 45.0%
! GCAAGCACTTTTCGCCTAAT+TGG + chr7_3:31610757-31610776 None:intergenic 45.0%
GCTTGCTTCGTGCTGTAAAA+AGG - chr7_3:31610771-31610790 Msa1101070:CDS 45.0%
GGTCATAACGTCCCAGTTAA+TGG - chr7_3:31610797-31610816 Msa1101070:CDS 45.0%
GGACTAGGCTTACCATTAAC+TGG + chr7_3:31610812-31610831 None:intergenic 45.0%
GCCAATAAAATGACCGGGTA+AGG + chr7_3:31611141-31611160 None:intergenic 45.0%
!!! AATTTAATATATTGATTTTT+TGG - chr7_3:31609071-31609090 Msa1101070:intron 5.0%
!! ATTGGTGGTGGTGGATTGGT+TGG + chr7_3:31608588-31608607 None:intergenic 50.0%
AACCAATCCACCACCACCAA+TGG - chr7_3:31608587-31608606 Msa1101070:three_prime_UTR 50.0%
!! TTCCATTGGTGGTGGTGGAT+TGG + chr7_3:31608592-31608611 None:intergenic 50.0%
TGAGAGTCTTCCATTGGTGG+TGG + chr7_3:31608600-31608619 None:intergenic 50.0%
! TCAGCACCGTCGATTGTCAT+CGG + chr7_3:31608648-31608667 None:intergenic 50.0%
AGCGAGAGAGTTGTCTGACA+CGG + chr7_3:31608737-31608756 None:intergenic 50.0%
GCTGTTACCGAGGTCATTAC+TGG - chr7_3:31608754-31608773 Msa1101070:three_prime_UTR 50.0%
!! GCTGCTGGTGCTGTTGATAA+TGG - chr7_3:31608885-31608904 Msa1101070:CDS 50.0%
! AACTCACCTTTTTGCCACGG+CGG + chr7_3:31609027-31609046 None:intergenic 50.0%
!! GAGAAGCACCGACAAAGACA+CGG - chr7_3:31609374-31609393 Msa1101070:intron 50.0%
ACCGGGACATGTCAAACACT+GGG - chr7_3:31609623-31609642 Msa1101070:intron 50.0%
TCCCAGTGTTTGACATGTCC+CGG + chr7_3:31609627-31609646 None:intergenic 50.0%
ACTGGGACATGTCTAATCGG+TGG - chr7_3:31609640-31609659 Msa1101070:intron 50.0%
TCCTACTTGACGTGGGACTT+AGG + chr7_3:31610174-31610193 None:intergenic 50.0%
GCATCTCATCCTACTTGACG+TGG + chr7_3:31610182-31610201 None:intergenic 50.0%
TGATAAGCACCCCAAGCACT+TGG + chr7_3:31610268-31610287 None:intergenic 50.0%
TGCTTGGAAGTGGGAAGTCA+TGG + chr7_3:31610314-31610333 None:intergenic 50.0%
AGGGCATACCAAAGGAACCA+TGG + chr7_3:31610726-31610745 None:intergenic 50.0%
CTTCGTGCTGTAAAAAGGGC+AGG - chr7_3:31610776-31610795 Msa1101070:CDS 50.0%
TAAACGAAAAGCTGCGCGCA+AGG - chr7_3:31610877-31610896 Msa1101070:CDS 50.0%
TGCGACTCTGTATCCTTACC+CGG - chr7_3:31611125-31611144 Msa1101070:CDS 50.0%
!! GAGTCTTCCATTGGTGGTGG+TGG + chr7_3:31608597-31608616 None:intergenic 55.0%
!! GTCATCGGCGCGATTTGTTG+CGG + chr7_3:31608633-31608652 None:intergenic 55.0%
! ATCGCGCCGATGACAATCGA+CGG - chr7_3:31608639-31608658 Msa1101070:three_prime_UTR 55.0%
GAGAGAGTTGTCTGACACGG+CGG + chr7_3:31608734-31608753 None:intergenic 55.0%
CAACTCTCTCGCTGTTACCG+AGG - chr7_3:31608744-31608763 Msa1101070:three_prime_UTR 55.0%
TGGTTGCAAGACCAAGGCCA+TGG - chr7_3:31608935-31608954 Msa1101070:CDS 55.0%
ACCGACAAAGACACGGACAC+CGG - chr7_3:31609381-31609400 Msa1101070:intron 55.0%
TCCGGTGTCCGTGTCTTTGT+CGG + chr7_3:31609385-31609404 None:intergenic 55.0%
ACGTGATGGCATGTCGGTGT+CGG - chr7_3:31609598-31609617 Msa1101070:intron 55.0%
CACCGGGACATGTCAAACAC+TGG - chr7_3:31609622-31609641 Msa1101070:intron 55.0%
! GTGGAGCGTCTGTGCTTCAT+AGG - chr7_3:31609659-31609678 Msa1101070:intron 55.0%
GCCTAAGTCCCACGTCAAGT+AGG - chr7_3:31610170-31610189 Msa1101070:intron 55.0%
GCACAGTATATAGGGAGGGC+AGG - chr7_3:31610982-31611001 Msa1101070:CDS 55.0%
!! ATCGGCGCGATTTGTTGCGG+TGG + chr7_3:31608630-31608649 None:intergenic 60.0%
!! GACCTCGCGGCGGGTTAAAA+CGG + chr7_3:31608697-31608716 None:intergenic 60.0%
AGAGTTGTCTGACACGGCGG+AGG + chr7_3:31608731-31608750 None:intergenic 60.0%
AACGCTTCTGCTGCTGCTGC+TGG - chr7_3:31608870-31608889 Msa1101070:CDS 60.0%
TTATCGTGGTTGCACCGCCG+TGG - chr7_3:31609010-31609029 Msa1101070:CDS 60.0%
GTTGCACCGCCGTGGCAAAA+AGG - chr7_3:31609018-31609037 Msa1101070:CDS 60.0%
!! CTCGCGGCGGGTTAAAACGG+AGG + chr7_3:31608694-31608713 None:intergenic 65.0%
!! CTCCGTTTTAACCCGCCGCG+AGG - chr7_3:31608692-31608711 Msa1101070:three_prime_UTR 65.0%
GTTGTCTGACACGGCGGAGG+CGG + chr7_3:31608728-31608747 None:intergenic 65.0%
!! GGCATGTCGGTGTCGGACAC+CGG - chr7_3:31609605-31609624 Msa1101070:intron 65.0%
!! GCATGTCGGTGTCGGACACC+GGG - chr7_3:31609606-31609625 Msa1101070:intron 65.0%
GTCTGACACGGCGGAGGCGG+CGG + chr7_3:31608725-31608744 None:intergenic 75.0%
! GCGGACGAGGACCTCGCGGC+GGG + chr7_3:31608706-31608725 None:intergenic 80.0%
! GGCGGACGAGGACCTCGCGG+CGG + chr7_3:31608707-31608726 None:intergenic 80.0%
! GGCGGCGGACGAGGACCTCG+CGG + chr7_3:31608710-31608729 None:intergenic 80.0%
! CACGGCGGAGGCGGCGGACG+AGG + chr7_3:31608719-31608738 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 31608510 31611255 31608510 ID=Msa1101070;Name=Msa1101070
chr7_3 mRNA 31608510 31611255 31608510 ID=Msa1101070-mRNA-1;Parent=Msa1101070;Name=Msa1101070-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|327|227
chr7_3 exon 31608510 31609086 31608510 ID=Msa1101070-mRNA-1:exon:6772;Parent=Msa1101070-mRNA-1
chr7_3 exon 31610726 31611255 31610726 ID=Msa1101070-mRNA-1:exon:6771;Parent=Msa1101070-mRNA-1
chr7_3 five_prime_UTR 31611160 31611255 31611160 ID=Msa1101070-mRNA-1:five_prime_utr;Parent=Msa1101070-mRNA-1
chr7_3 CDS 31610726 31611159 31610726 ID=Msa1101070-mRNA-1:cds;Parent=Msa1101070-mRNA-1
chr7_3 CDS 31608837 31609086 31608837 ID=Msa1101070-mRNA-1:cds;Parent=Msa1101070-mRNA-1
chr7_3 three_prime_UTR 31608510 31608836 31608510 ID=Msa1101070-mRNA-1:three_prime_utr;Parent=Msa1101070-mRNA-1
Gene Sequence

>Msa1101070

ATGGAAGACTCTCAACAACAACCACCGCAACAAATCGCGCCGATGACAATCGACGGTGCTGACGAAGACTTAGCCCTCGCTAACTCCTCCGTTTTAACCCGCCGCGAGGTCCTCGTCCGCCGCCTCCGCCGTGTCAGACAACTCTCTCGCTGTTACCGAGGTCATTACTGGGCGTTAATGGAAGAGCTTAAGGCTAAGTACAGAGAGTATTACTGGACTTATGGAAGGAGTCCGTTCAAGGAAGATGAAGAAAGAAATTCGGGTAACGCTTCTGCTGCTGCTGCTGGTGCTGTTGATAATGGTGATGATTTTGTTCGGTGTGCTTCTAGTGGTTGCAAGACCAAGGCCATGGCTCTTATTAAGTATTGTCATGCGCATATTTTGTCTGATCCTAATCAGAAGCTTTATCGTGGTTGCACCGCCGTGGCAAAAAGTTTGCCAACAGGGCCAGTCTTTTGTAATAAGCCAGTATTGAGATCCATGGTTCCTTTGGTATGCCCTGCTCATTACCAATTAGGCGAAAAGTGCTTGCTTCGTGCTGTAAAAAGGGCAGGTCATAACGTCCCAGTTAATGGTAAGCCTAGTCCGCAGTTACATCTATTAATTCCTGAATTTGTCCGCCAAATTCAAAATAAACGAAAAGCTGCGCGCAAGGCAGCTTTACCTAAACCTGAGACTGATTAA
Protein sequence

>Msa1101070

MEDSQQQPPQQIAPMTIDGADEDLALANSSVLTRREVLVRRLRRVRQLSRCYRGHYWALMEELKAKYREYYWTYGRSPFKEDEERNSGNASAAAAGAVDNGDDFVRCASSGCKTKAMALIKYCHAHILSDPNQKLYRGCTAVAKSLPTGPVFCNKPVLRSMVPLVCPAHYQLGEKCLLRAVKRAGHNVPVNGKPSPQLHLLIPEFVRQIQNKRKAARKAALPKPETD*