AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1118120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118120 MtrunA17_Chr7g0254511 97.521 484 12 0 1 484 1 484 0.0 954
Msa1118120 MtrunA17_Chr7g0254521 72.688 465 126 1 24 487 25 489 0.0 712
Msa1118120 MtrunA17_Chr6g0460541 76.049 405 95 1 87 489 3 407 0.0 627
Msa1118120 MtrunA17_Chr7g0254531 73.367 398 105 1 89 485 1 398 0.0 591
Msa1118120 MtrunA17_Chr7g0254501 91.167 317 28 0 173 489 1 317 0.0 560
Msa1118120 MtrunA17_Chr5g0412541 50.522 479 234 3 4 480 11 488 1.75e-168 484
Msa1118120 MtrunA17_Chr3g0132531 49.576 472 235 3 12 481 29 499 6.16e-162 468
Msa1118120 MtrunA17_Chr8g0368001 46.288 458 245 1 30 486 50 507 7.28e-150 437
Msa1118120 MtrunA17_Chr1g0202761 46.121 464 247 2 25 486 38 500 3.80e-146 427
Msa1118120 MtrunA17_Chr5g0412771 47.265 457 236 2 31 482 57 513 3.10e-142 418
Msa1118120 MtrunA17_Chr4g0024501 45.992 474 244 3 12 473 36 509 5.32e-141 415
Msa1118120 MtrunA17_Chr2g0322671 45.570 474 246 3 12 473 24 497 3.50e-137 405
Msa1118120 MtrunA17_Chr5g0412571 48.622 399 199 4 87 481 38 434 6.36e-132 389
Msa1118120 MtrunA17_Chr6g0460531 69.847 262 71 2 5 265 6 260 6.30e-131 380
Msa1118120 MtrunA17_Chr2g0288281 48.320 387 198 1 89 473 1 387 1.72e-128 379
Msa1118120 MtrunA17_Chr1g0154441 48.320 387 199 1 89 474 1 387 4.58e-128 377
Msa1118120 MtrunA17_Chr7g0248051 45.011 451 248 0 22 472 62 512 3.30e-127 380
Msa1118120 MtrunA17_Chr6g0468531 38.958 480 281 4 10 479 5 482 4.69e-116 350
Msa1118120 MtrunA17_Chr6g0468521 38.631 453 277 1 22 474 23 474 7.53e-114 344
Msa1118120 MtrunA17_Chr5g0442331 41.702 470 259 7 20 479 31 495 9.98e-112 340
Msa1118120 MtrunA17_Chr3g0132541 39.789 475 213 7 2 464 8 421 3.85e-105 320
Msa1118120 MtrunA17_Chr6g0468511 36.947 452 284 1 22 473 23 473 6.34e-104 318
Msa1118120 MtrunA17_Chr6g0460521 75.113 221 55 0 264 484 1 221 3.77e-100 300
Msa1118120 MtrunA17_Chr6g0483361 35.575 461 292 3 14 470 10 469 8.78e-91 285
Msa1118120 MtrunA17_Chr6g0483381 35.054 465 297 3 10 470 8 471 8.70e-90 282
Msa1118120 MtrunA17_Chr7g0251331 35.818 483 307 2 6 486 3 484 7.05e-88 277
Msa1118120 MtrunA17_Chr1g0208791 33.917 457 294 4 12 462 23 477 2.26e-85 271
Msa1118120 MtrunA17_Chr6g0477961 35.892 443 277 4 33 470 31 471 3.03e-84 268
Msa1118120 MtrunA17_Chr6g0483331 34.348 460 293 6 25 478 7 463 2.36e-83 265
Msa1118120 MtrunA17_Chr6g0483341 32.906 468 303 5 14 472 21 486 2.34e-82 263
Msa1118120 MtrunA17_Chr2g0317131 37.391 460 274 3 13 461 20 476 3.99e-82 263
Msa1118120 MtrunA17_Chr6g0483351 37.270 381 235 2 89 465 1 381 8.66e-80 255
Msa1118120 MtrunA17_Chr2g0317171 36.247 469 281 5 6 461 7 470 3.56e-79 255
Msa1118120 MtrunA17_Chr8g0392601 32.889 450 289 4 30 472 13 456 1.09e-78 253
Msa1118120 MtrunA17_Chr2g0317161 36.404 456 276 4 17 461 12 464 2.11e-77 250
Msa1118120 MtrunA17_Chr2g0317151 38.215 437 262 2 30 461 33 466 2.70e-77 250
Msa1118120 MtrunA17_Chr1g0208821 32.981 473 307 4 6 470 13 483 3.83e-77 250
Msa1118120 MtrunA17_Chr6g0449061 32.479 468 307 4 18 479 18 482 4.57e-76 247
Msa1118120 MtrunA17_Chr2g0317221 40.157 381 220 3 86 461 7 384 4.55e-74 239
Msa1118120 MtrunA17_Chr1g0208881 32.548 467 313 1 6 470 1 467 4.06e-73 239
Msa1118120 MtrunA17_Chr7g0254491 91.379 116 10 0 1 116 1 116 3.92e-72 224
Msa1118120 MtrunA17_Chr1g0208891 33.546 471 302 3 6 470 1 466 2.46e-71 234
Msa1118120 MtrunA17_Chr6g0483161 33.158 380 233 2 87 464 1 361 1.54e-67 221
Msa1118120 MtrunA17_Chr3g0140931 28.387 465 315 5 14 468 35 491 2.68e-62 212
Msa1118120 MtrunA17_Chr7g0251321 32.152 395 247 2 89 481 1 376 1.03e-61 206
Msa1118120 MtrunA17_Chr5g0428251 25.470 479 346 4 7 479 32 505 3.93e-52 185
Msa1118120 MtrunA17_Chr5g0397991 27.191 445 314 4 30 468 65 505 1.85e-51 183
Msa1118120 MtrunA17_Chr1g0203791 27.079 469 316 5 14 468 48 504 5.79e-51 181
Msa1118120 MtrunA17_Chr5g0440661 30.848 389 188 10 89 472 1 313 3.12e-49 171
Msa1118120 MtrunA17_Chr6g0467321 26.200 500 340 6 8 483 12 506 1.13e-48 175
Msa1118120 MtrunA17_Chr8g0364761 33.663 303 187 4 7 296 21 322 2.28e-48 169
Msa1118120 MtrunA17_Chr6g0473701 26.623 462 325 5 27 480 125 580 1.38e-47 174
Msa1118120 MtrunA17_Chr4g0013031 25.108 462 338 3 26 479 62 523 2.58e-46 169
Msa1118120 MtrunA17_Chr8g0364751 48.276 174 90 0 301 474 6 179 3.26e-44 154
Msa1118120 MtrunA17_Chr8g0389801 27.009 448 293 8 30 460 23 453 7.82e-39 147
Msa1118120 MtrunA17_Chr6g0483264 34.804 204 133 0 267 470 1 204 5.18e-34 127
Msa1118120 MtrunA17_Chr2g0295631 23.218 435 308 4 60 472 50 480 2.43e-30 123
Msa1118120 MtrunA17_Chr6g0483221 35.714 154 99 0 310 463 2 155 9.90e-30 114
Msa1118120 MtrunA17_Chr6g0483241 38.798 183 106 4 87 265 1 181 4.12e-28 110
Msa1118120 MtrunA17_Chr6g0460551 73.913 69 17 1 19 86 25 93 6.27e-26 102
Msa1118120 MtrunA17_Chr6g0483261 34.783 161 105 0 310 470 2 162 1.31e-23 97.8
Msa1118120 MtrunA17_Chr5g0442311 33.149 181 115 3 89 265 1 179 3.61e-23 96.7
Msa1118120 MtrunA17_Chr6g0487091 26.935 323 217 4 170 486 93 402 2.95e-22 99.0
Msa1118120 MtrunA17_Chr5g0398571 29.439 214 151 0 254 467 73 286 2.04e-19 89.0
Msa1118120 MtrunA17_Chr5g0412561 49.315 73 37 0 14 86 20 92 2.35e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1118120 AT1G33110.1 65.984 488 162 3 2 485 4 491 0.0 674
Msa1118120 AT1G33100.1 63.768 483 174 1 4 485 6 488 0.0 659
Msa1118120 AT1G33090.1 62.757 486 177 3 4 485 6 491 0.0 633
Msa1118120 AT1G33080.1 62.268 485 179 2 4 484 6 490 0.0 628
Msa1118120 AT1G33080.2 63.656 465 165 2 3 463 5 469 0.0 615
Msa1118120 AT1G33110.2 67.000 400 128 3 2 397 4 403 0.0 553
Msa1118120 AT5G65380.1 50.636 472 231 2 5 474 11 482 1.02e-164 474
Msa1118120 AT5G44050.1 50.000 472 230 3 10 477 19 488 6.26e-164 473
Msa1118120 AT1G47530.1 46.921 471 244 2 10 474 9 479 7.66e-151 439
Msa1118120 AT4G00350.1 47.297 444 234 0 31 474 88 531 6.69e-150 439
Msa1118120 AT5G10420.1 47.609 481 245 5 5 482 13 489 3.49e-148 432
Msa1118120 AT1G12950.1 47.682 453 236 1 31 482 67 519 8.88e-144 422
Msa1118120 AT3G26590.1 47.523 444 232 1 31 473 45 488 2.37e-143 421
Msa1118120 AT3G03620.2 44.255 470 261 1 15 483 21 490 2.01e-141 416
Msa1118120 AT3G03620.1 44.255 470 261 1 15 483 21 490 2.01e-141 416
Msa1118120 AT4G25640.1 46.764 479 249 2 10 482 7 485 1.04e-140 414
Msa1118120 AT4G25640.2 46.764 479 249 2 10 482 7 485 4.32e-140 413
Msa1118120 AT5G17700.2 43.067 476 269 2 2 475 4 479 7.89e-140 412
Msa1118120 AT5G17700.1 43.067 476 269 2 2 475 4 479 7.89e-140 412
Msa1118120 AT1G11670.1 46.966 445 234 1 31 473 50 494 1.67e-139 411
Msa1118120 AT1G61890.1 45.553 461 245 2 19 473 31 491 2.36e-139 410
Msa1118120 AT5G38030.1 45.595 454 246 1 31 483 45 498 2.28e-138 408
Msa1118120 AT1G23300.1 45.969 459 243 2 31 488 44 498 1.91e-137 406
Msa1118120 AT3G21690.1 46.799 453 239 1 24 474 45 497 2.87e-136 403
Msa1118120 AT1G61890.2 44.444 468 252 3 5 464 15 482 4.39e-135 399
Msa1118120 AT1G47530.2 48.964 386 196 1 89 473 1 386 1.62e-130 384
Msa1118120 AT4G25640.3 47.552 429 219 2 10 432 7 435 1.21e-127 379
Msa1118120 AT3G26590.2 48.187 386 199 1 89 473 1 386 7.37e-126 372
Msa1118120 AT4G21910.2 42.082 461 260 3 19 473 41 500 2.88e-125 375
Msa1118120 AT4G21910.4 42.082 461 260 3 19 473 41 500 2.88e-125 375
Msa1118120 AT4G21910.3 40.956 481 261 4 15 473 19 498 4.36e-125 374
Msa1118120 AT4G21910.1 40.956 481 261 4 15 473 19 498 4.36e-125 374
Msa1118120 AT4G21903.1 44.131 426 233 4 51 473 73 496 4.90e-122 366
Msa1118120 AT4G21903.2 42.825 446 243 6 51 489 73 513 4.57e-119 359
Msa1118120 AT1G61890.3 44.186 430 234 2 19 442 31 460 1.30e-118 357
Msa1118120 AT3G59030.1 40.825 485 270 4 14 483 22 504 4.80e-117 353
Msa1118120 AT4G21903.3 44.845 388 210 3 89 473 1 387 9.42e-116 347
Msa1118120 AT5G17700.3 42.714 398 226 2 2 397 4 401 1.99e-111 336
Msa1118120 AT5G52450.1 34.426 488 303 4 2 485 8 482 4.75e-89 281
Msa1118120 AT2G34360.1 35.095 473 289 6 20 485 16 477 3.54e-86 273
Msa1118120 AT3G59030.2 37.890 417 235 4 1 394 1 416 3.15e-84 266
Msa1118120 AT1G73700.1 36.129 465 289 5 20 479 13 474 5.35e-83 265
Msa1118120 AT1G71140.1 33.913 460 294 3 17 470 12 467 3.43e-80 258
Msa1118120 AT1G66760.2 32.285 477 310 4 10 480 10 479 2.94e-75 245
Msa1118120 AT2G34360.2 31.933 476 266 4 16 485 12 435 3.26e-75 243
Msa1118120 AT1G15150.1 34.354 457 285 6 31 480 31 479 2.78e-74 243
Msa1118120 AT1G66760.1 32.609 460 297 4 10 463 10 462 6.43e-73 238
Msa1118120 AT1G15160.1 31.884 483 305 7 14 480 5 479 4.43e-72 237
Msa1118120 AT1G66780.1 34.507 426 275 2 48 470 52 476 1.01e-71 236
Msa1118120 AT2G04080.1 33.404 473 293 9 14 470 2 468 1.24e-71 235
Msa1118120 AT1G15170.1 33.610 482 301 8 1 470 1 475 3.63e-71 234
Msa1118120 AT2G04080.2 33.049 469 292 9 14 466 2 464 3.78e-69 228
Msa1118120 AT2G04040.1 33.482 448 284 7 31 470 27 468 5.11e-69 228
Msa1118120 AT2G04070.1 32.759 464 301 5 16 470 7 468 5.62e-69 228
Msa1118120 AT3G23560.1 33.260 454 292 2 19 461 18 471 2.95e-68 226
Msa1118120 AT2G04050.1 33.404 470 297 7 10 470 6 468 2.05e-67 224
Msa1118120 AT1G64820.1 30.942 446 300 4 30 470 28 470 5.87e-67 224
Msa1118120 AT3G23550.1 33.790 438 280 6 30 461 30 463 6.15e-66 220
Msa1118120 AT2G04100.1 31.637 452 299 4 25 470 24 471 3.64e-63 213
Msa1118120 AT1G15180.1 33.557 447 285 6 31 470 35 476 4.12e-63 213
Msa1118120 AT2G04090.1 31.556 450 290 6 31 470 30 471 4.31e-62 210
Msa1118120 AT2G04100.2 31.767 447 295 4 25 465 24 466 8.41e-62 209
Msa1118120 AT1G15150.2 35.054 368 227 5 120 480 1 363 2.39e-60 202
Msa1118120 AT1G15170.2 34.358 358 226 4 120 470 1 356 2.20e-58 197
Msa1118120 AT2G38510.1 25.108 462 339 3 27 483 2 461 4.08e-50 178
Msa1118120 AT1G66760.3 32.036 334 225 1 132 463 1 334 1.57e-49 173
Msa1118120 AT1G66760.4 30.435 391 259 4 10 394 10 393 6.13e-49 173
Msa1118120 AT1G58340.1 24.944 449 327 3 30 472 57 501 5.90e-48 173
Msa1118120 AT3G23560.2 32.041 387 252 2 19 394 18 404 1.15e-47 170
Msa1118120 AT4G29140.1 25.153 489 344 6 1 479 29 505 2.46e-41 155
Msa1118120 AT1G71870.1 24.206 504 342 9 7 483 7 497 6.52e-41 154
Msa1118120 AT5G19700.1 24.842 475 336 5 7 468 9 475 1.99e-39 149
Msa1118120 AT5G52050.1 24.886 438 287 6 55 467 59 479 2.52e-34 135
Msa1118120 AT5G49130.1 25.667 487 334 8 11 478 12 489 2.50e-32 129
Msa1118120 AT4G23030.1 24.536 485 354 5 6 483 8 487 2.70e-32 129
Msa1118120 AT1G15180.2 31.915 282 183 5 120 394 1 280 3.71e-29 119
Msa1118120 AT4G22790.1 25.275 455 306 11 50 488 49 485 6.58e-28 116
Msa1118120 AT2G04066.1 38.400 125 76 1 347 470 39 163 3.87e-24 99.4

Find 122 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTATTGGATGCCAAATT+TGG 0.094262 7_3:-66663608 Msa1118120:CDS
CCATTTATTAACACGATTTC+TGG 0.107535 7_3:+66661491 None:intergenic
CCAGAAATCGTGTTAATAAA+TGG 0.153553 7_3:-66661491 Msa1118120:CDS
CATTTCCACTTCTTCTACTA+TGG 0.160377 7_3:+66664680 None:intergenic
TGGCCAAGTGCCACTAAAAT+TGG 0.210821 7_3:+66663859 None:intergenic
AAGTTCAAGTTTGGCCTTAA+TGG 0.236077 7_3:-66663652 Msa1118120:CDS
AAAACAAGCTGCCCCAAATT+TGG 0.237889 7_3:+66663595 None:intergenic
ATCAAGCTTTCTTTATCTTC+TGG 0.246424 7_3:-66663502 Msa1118120:CDS
TTGGACACTGTTCATTAATA+TGG 0.249353 7_3:+66662727 None:intergenic
TGTGATTATCATAAGCATTA+TGG 0.251121 7_3:+66661646 None:intergenic
TACCTAATTGGTATTCCTAT+TGG 0.253015 7_3:-66662341 Msa1118120:CDS
CATTAGCCACATCTGGATTT+GGG 0.265681 7_3:+66662783 None:intergenic
CCATTGATGCTCTTGCTATA+TGG 0.272797 7_3:-66663290 Msa1118120:intron
GCATTAGCCACATCTGGATT+TGG 0.277971 7_3:+66662782 None:intergenic
CATATATATGTTGCACTAAT+AGG 0.287588 7_3:+66664741 None:intergenic
TGTTCTTGCTTTGTGCTTGT+AGG 0.291347 7_3:+66663743 None:intergenic
CAATCCTTCATTGGTCATAT+TGG 0.293119 7_3:-66664492 Msa1118120:CDS
CAGGTTTGCAAATGGTATTC+TGG 0.301200 7_3:-66664424 Msa1118120:intron
TGTTGAAAACCTATTGAATA+TGG 0.301753 7_3:+66664536 None:intergenic
ATAGGCTGCTATTACCTAAT+TGG 0.305303 7_3:-66662353 Msa1118120:CDS
AACAGGCCAAAGATCTTTAA+AGG 0.330427 7_3:+66663525 None:intergenic
GCTTATTGGATGCCAAATTT+GGG 0.332361 7_3:-66663607 Msa1118120:CDS
GAAAATATTGCACAAGTTGC+TGG 0.335153 7_3:-66663832 Msa1118120:CDS
TGCAATGACATCCATCCTTT+TGG 0.335752 7_3:-66663629 Msa1118120:CDS
TGTAAGCAAGTCTTCCTCTT+AGG 0.344861 7_3:+66662813 None:intergenic
GAGAAACCCTTCCATGTATC+AGG 0.346664 7_3:+66663556 None:intergenic
GTTTGGATTGGAATGTTGTT+TGG 0.348970 7_3:-66661683 Msa1118120:CDS
TATCTTCTGGAGCTATGTTA+TGG 0.352221 7_3:-66663489 Msa1118120:intron
TTAACTGTTAAGTTCAAGTT+TGG 0.352285 7_3:-66663661 Msa1118120:CDS
TATTCTCCATTTGCAAGTTA+AGG 0.357518 7_3:-66662303 Msa1118120:intron
TCTTGATATTCTTCTTTAGT+TGG 0.358461 7_3:+66661425 None:intergenic
TGGATGTCATTGCACCATTA+AGG 0.369349 7_3:+66663638 None:intergenic
TCAGTGAAGAAAGTCTAATA+AGG 0.387668 7_3:-66661391 Msa1118120:three_prime_UTR
CATAGTAGAAGAAGTGGAAA+TGG 0.391136 7_3:-66664679 Msa1118120:exon
CTCCAATAGGAATACCAATT+AGG 0.396168 7_3:+66662339 None:intergenic
GATAAAGAAAGCTTGATAAC+AGG 0.397818 7_3:+66663508 None:intergenic
TTGTTATGACTGTCCTAGTC+AGG 0.399000 7_3:-66664443 Msa1118120:CDS
CAGGCCCTGCCATATTCAAT+AGG 0.402161 7_3:-66664545 Msa1118120:CDS
GGCTCTTCTTCCTCCTCTGA+AGG 0.408718 7_3:+66664615 None:intergenic
CCATATAGCAAGAGCATCAA+TGG 0.415111 7_3:+66663290 None:intergenic
GAAGAAGTGGAAATGGGATC+AGG 0.419669 7_3:-66664672 Msa1118120:exon
TTTGTGCTTGTAGGAACATT+TGG 0.420861 7_3:+66663752 None:intergenic
TTGAAAATTGTAGTTGGGTA+TGG 0.421860 7_3:-66664019 Msa1118120:intron
CCAACTGCATTAGCCACATC+TGG 0.423816 7_3:+66662776 None:intergenic
TTACCAAGCACAACTCCAAT+AGG 0.429954 7_3:+66662326 None:intergenic
AAGGGCATAAGCAGCTAATT+CGG 0.440329 7_3:+66664464 None:intergenic
GTAGTTGCTCAATCCTTCAT+TGG 0.440406 7_3:-66664501 Msa1118120:CDS
GAATTACCTTAACTTGCAAA+TGG 0.446854 7_3:+66662297 None:intergenic
CAGAAATCGTGTTAATAAAT+GGG 0.446923 7_3:-66661490 Msa1118120:CDS
AAGAAGAGCCACTAAGGAAA+AGG 0.447419 7_3:-66664602 Msa1118120:CDS
TTGCTGGAAGCATTTCAATA+TGG 0.449250 7_3:-66663816 Msa1118120:CDS
AGCATTTCAATATGGTCAAT+AGG 0.456375 7_3:-66663808 Msa1118120:CDS
ACACTATGTGGACAAGCATA+TGG 0.467239 7_3:-66663982 Msa1118120:CDS
ATAGGTGTTTCTGTTGGAGC+TGG 0.468391 7_3:-66662404 Msa1118120:intron
TTTGCAGCCTCAACATCAAT+GGG 0.469922 7_3:-66663165 Msa1118120:intron
GAAAACCTATTGAATATGGC+AGG 0.470460 7_3:+66664540 None:intergenic
GCTCTTCTTCCTCCTCTGAA+GGG 0.476165 7_3:+66664616 None:intergenic
TTGATCTTTCTGTTCCTAAG+AGG 0.477472 7_3:-66662827 Msa1118120:CDS
AAGTGCCACTAAAATTGGTT+TGG 0.478153 7_3:+66663864 None:intergenic
GAGTGTATCTTCAAAGATCA+TGG 0.479506 7_3:-66663936 Msa1118120:CDS
GTGTTTCTGTTGGAGCTGGC+TGG 0.479528 7_3:-66662400 Msa1118120:CDS
AGCCACTAAGGAAAAGGGTA+TGG 0.482191 7_3:-66664596 Msa1118120:CDS
AAAATCATATCTAGCCTCTA+GGG 0.484679 7_3:+66661317 None:intergenic
AGTGTCCAACCTGTGCTCTC+TGG 0.488293 7_3:-66662713 Msa1118120:intron
GTGCTCACATCATTTGCCAT+TGG 0.492593 7_3:-66662863 Msa1118120:CDS
TAATCACATTTAAGACTGAC+TGG 0.493785 7_3:-66661631 Msa1118120:CDS
GCAAAACAATATGATATGCT+TGG 0.495199 7_3:-66663958 Msa1118120:CDS
GGGAAGAAAGCAAGAAATTG+TGG 0.497231 7_3:-66664575 Msa1118120:CDS
AGGAGGAAGAAGAGCCACTA+AGG 0.500928 7_3:-66664608 Msa1118120:CDS
ATTCCTATTGGAGTTGTGCT+TGG 0.501302 7_3:-66662329 Msa1118120:CDS
AAGAAATTGTGGATAGTAGC+AGG 0.502559 7_3:-66664564 Msa1118120:CDS
AGAAGAGCCACTAAGGAAAA+GGG 0.507893 7_3:-66664601 Msa1118120:CDS
TGAACCAATATGACCAATGA+AGG 0.509207 7_3:+66664488 None:intergenic
ATAGTAGAAGAAGTGGAAAT+GGG 0.513164 7_3:-66664678 Msa1118120:exon
TTGGCATCCAATAAGCCAAA+AGG 0.517836 7_3:+66663614 None:intergenic
TGACAAAATGTCCTGATACA+TGG 0.517890 7_3:-66663567 Msa1118120:CDS
TTGGTTTCTTTGCTGCAGCT+AGG 0.518538 7_3:-66663123 Msa1118120:intron
GTTAGGGTGTCGAATGAACT+AGG 0.518686 7_3:-66662929 Msa1118120:CDS
TTTCCCACCCATTGATGTTG+AGG 0.520512 7_3:+66663158 None:intergenic
CAGCTCCATAGTAGAAGAAG+TGG 0.525322 7_3:-66664685 Msa1118120:five_prime_UTR
TTGTGAATAGGTGTTTCTGT+TGG 0.527931 7_3:-66662410 Msa1118120:intron
GTCCTAGTCAGGTTTGCAAA+TGG 0.528064 7_3:-66664432 Msa1118120:CDS
CCAGATGTGGCTAATGCAGT+TGG 0.534123 7_3:-66662776 Msa1118120:CDS
TGTTAATAAATGGGCTGTAG+TGG 0.537932 7_3:-66661481 Msa1118120:CDS
GATTCTTCTAACAGGGAACA+TGG 0.541639 7_3:-66663328 Msa1118120:CDS
ATGAATCAAACAACACATCA+AGG 0.547424 7_3:-66661452 Msa1118120:CDS
AATTGGAAGAAGAAGAATTG+AGG 0.547786 7_3:+66663897 None:intergenic
GTGTCGAATGAACTAGGAAG+AGG 0.554484 7_3:-66662923 Msa1118120:CDS
TAAGACTGACTGGGACAAGC+AGG 0.560914 7_3:-66661621 Msa1118120:intron
TATTAATCAAGGTTTAAATG+AGG 0.568253 7_3:-66664767 Msa1118120:five_prime_UTR
GTTGTAGCATATGTCAACAT+AGG 0.569004 7_3:-66662371 Msa1118120:CDS
CAAGAATAGCACAAATCCAA+TGG 0.586224 7_3:+66662847 None:intergenic
AAAATGTCCTGATACATGGA+AGG 0.587310 7_3:-66663563 Msa1118120:CDS
TACCATTTGCAAACCTGACT+AGG 0.588271 7_3:+66664430 None:intergenic
ATTAGCCACATCTGGATTTG+GGG 0.588466 7_3:+66662784 None:intergenic
GCAGCCTCAACATCAATGGG+TGG 0.590413 7_3:-66663162 Msa1118120:intron
GCAGCATCAAAACCCTTCAG+AGG 0.592540 7_3:-66664628 Msa1118120:CDS
GCCACTAAGGAAAAGGGTAT+GGG 0.595578 7_3:-66664595 Msa1118120:CDS
AAATATTTACCAGAGAGCAC+AGG 0.596757 7_3:+66662704 None:intergenic
CAGCCTCAACATCAATGGGT+GGG 0.609170 7_3:-66663161 Msa1118120:CDS
TAGCTGCAGCAAAGAAACCA+AGG 0.611276 7_3:+66663125 None:intergenic
CTTATTGGATGCCAAATTTG+GGG 0.612153 7_3:-66663606 Msa1118120:CDS
AACTAAAGAAGAATATCAAG+AGG 0.616189 7_3:-66661423 Msa1118120:exon
TGGGAAATGATGATAGCCCT+TGG 0.617562 7_3:-66663142 Msa1118120:CDS
TGACTAGGACAGTCATAACA+AGG 0.618469 7_3:+66664445 None:intergenic
GCATCAAAACCCTTCAGAGG+AGG 0.620658 7_3:-66664625 Msa1118120:CDS
AAATCATATCTAGCCTCTAG+GGG 0.624956 7_3:+66661318 None:intergenic
ACTAGGAAGAGGGAGTTCCA+AGG 0.626189 7_3:-66662912 Msa1118120:CDS
TGTCGAATGAACTAGGAAGA+GGG 0.634314 7_3:-66662922 Msa1118120:CDS
CATCCAATAAGCCAAAAGGA+TGG 0.636104 7_3:+66663618 None:intergenic
TTTCACCCCAAATCCAGATG+TGG 0.637534 7_3:-66662789 Msa1118120:CDS
TCTAGTCAAAATACCCCTAG+AGG 0.644635 7_3:-66661331 Msa1118120:three_prime_UTR
AGCTGCAGCAAAGAAACCAA+GGG 0.647868 7_3:+66663126 None:intergenic
AAATGTCCTGATACATGGAA+GGG 0.649772 7_3:-66663562 Msa1118120:CDS
AATCACATTTAAGACTGACT+GGG 0.658771 7_3:-66661630 Msa1118120:CDS
AAAACCTATTGAATATGGCA+GGG 0.658833 7_3:+66664541 None:intergenic
TTGAAATTGAAACTGCTGCA+AGG 0.660479 7_3:+66663707 None:intergenic
ATTTACCAGAGAGCACAGGT+TGG 0.672429 7_3:+66662708 None:intergenic
TGTGAGCACTGTTATCACAA+TGG 0.707567 7_3:+66662877 None:intergenic
GACTAGGACAGTCATAACAA+GGG 0.709723 7_3:+66664446 None:intergenic
AGTGCATTAGAAACACTATG+TGG 0.716160 7_3:-66663994 Msa1118120:CDS
AATATGGAGAATGATAACAA+AGG 0.716405 7_3:+66662743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTTACTAAATAACAT+AGG - chr7_3:66664147-66664166 Msa1118120:intron 10.0%
!! TTTATATATAAACAAAGTAA+CGG + chr7_3:66664175-66664194 None:intergenic 10.0%
!!! TCAATTTTTAAGATTATGTA+TGG - chr7_3:66662580-66662599 Msa1118120:intron 15.0%
!! CTCTTTATATTATATATATG+TGG + chr7_3:66664244-66664263 None:intergenic 15.0%
!!! TAATAACATATGTATTTTAC+AGG - chr7_3:66664499-66664518 Msa1118120:CDS 15.0%
!!! ATTTTATTTTTTGAAGGAAT+TGG + chr7_3:66664768-66664787 None:intergenic 15.0%
!! TATTAATCAAGGTTTAAATG+AGG - chr7_3:66661255-66661274 Msa1118120:three_prime_UTR 20.0%
!!! AATGTAATAATTTAGAGCAA+AGG - chr7_3:66662977-66662996 Msa1118120:intron 20.0%
!! AAAATTGAATCAATAAGCAA+TGG + chr7_3:66663367-66663386 None:intergenic 20.0%
!!! ATTTTAGTCCTTAAGATTAT+AGG - chr7_3:66663411-66663430 Msa1118120:intron 20.0%
!!! ACAACATTTTTAGATGAATT+TGG + chr7_3:66663593-66663612 None:intergenic 20.0%
!!! TAATTCATTTGTCATTTACA+TGG - chr7_3:66663742-66663761 Msa1118120:CDS 20.0%
!! GATGAAAAAATATGTTTGAT+AGG + chr7_3:66664053-66664072 None:intergenic 20.0%
!! ATAACATATCAACATTACAT+TGG + chr7_3:66664126-66664145 None:intergenic 20.0%
!! AATGATTAAAGAGATATTGT+TGG - chr7_3:66664288-66664307 Msa1118120:intron 20.0%
!! CTGCTTAAAATTATAAGAAA+CGG + chr7_3:66664475-66664494 None:intergenic 20.0%
! CATATATATGTTGCACTAAT+AGG + chr7_3:66661284-66661303 None:intergenic 25.0%
! TGTTGAAAACCTATTGAATA+TGG + chr7_3:66661489-66661508 None:intergenic 25.0%
!! TTCAATAGGTTTTCAACATT+TGG - chr7_3:66661491-66661510 Msa1118120:CDS 25.0%
! CTATAATACAATTGTGATTG+AGG + chr7_3:66661707-66661726 None:intergenic 25.0%
! CTATAATACTATTGCAATTG+AGG + chr7_3:66661837-66661856 None:intergenic 25.0%
! TGCAAATTGAAAATTGTAGT+TGG - chr7_3:66661997-66662016 Msa1118120:intron 25.0%
! GCAAATTGAAAATTGTAGTT+GGG - chr7_3:66661998-66662017 Msa1118120:intron 25.0%
!! GGTTTGGTAAAAATGTAAAT+TGG + chr7_3:66662145-66662164 None:intergenic 25.0%
!! TTTTACCAAACCAATTTTAG+TGG - chr7_3:66662153-66662172 Msa1118120:intron 25.0%
!!! CAATACATGTTTTTATGTCA+TGG - chr7_3:66662335-66662354 Msa1118120:CDS 25.0%
!! TTAACTGTTAAGTTCAAGTT+TGG - chr7_3:66662361-66662380 Msa1118120:CDS 25.0%
!! GTTTGTAATCAAAGTTTGTT+TGG - chr7_3:66662602-66662621 Msa1118120:intron 25.0%
!! ACTTTTACAGTCTTGAAATA+TGG - chr7_3:66662657-66662676 Msa1118120:intron 25.0%
!! TCTTGTCAGTACTAAATTTT+AGG - chr7_3:66663047-66663066 Msa1118120:intron 25.0%
!!! TACATTTTTTTTGCAGTGTT+AGG - chr7_3:66663076-66663095 Msa1118120:intron 25.0%
!!! ACATTTTTTTTGCAGTGTTA+GGG - chr7_3:66663077-66663096 Msa1118120:intron 25.0%
! AATATGGAGAATGATAACAA+AGG + chr7_3:66663282-66663301 None:intergenic 25.0%
! GCTATTTGATCTCTAATTAA+TGG + chr7_3:66663468-66663487 None:intergenic 25.0%
! CTAAAAATGTTGTTGTGAAT+AGG - chr7_3:66663600-66663619 Msa1118120:CDS 25.0%
!!! CTTTTTGGTAATTATGTCAT+TGG - chr7_3:66663950-66663969 Msa1118120:CDS 25.0%
! CTTTAATCATTATGCGATAT+AGG + chr7_3:66664279-66664298 None:intergenic 25.0%
! TGTGATTATCATAAGCATTA+TGG + chr7_3:66664379-66664398 None:intergenic 25.0%
! CAGAAATCGTGTTAATAAAT+GGG - chr7_3:66664532-66664551 Msa1118120:CDS 25.0%
! TCTTGATATTCTTCTTTAGT+TGG + chr7_3:66664600-66664619 None:intergenic 25.0%
! AACTAAAGAAGAATATCAAG+AGG - chr7_3:66664599-66664618 Msa1118120:CDS 25.0%
ATAGTAGAAGAAGTGGAAAT+GGG - chr7_3:66661344-66661363 Msa1118120:three_prime_UTR 30.0%
AAAACCTATTGAATATGGCA+GGG + chr7_3:66661484-66661503 None:intergenic 30.0%
! GTTTTCAACATTTGGTATCA+TGG - chr7_3:66661499-66661518 Msa1118120:CDS 30.0%
GATTGTGATTTAAGAGATTG+AGG + chr7_3:66661637-66661656 None:intergenic 30.0%
CAATTGTATTTGTGACATCA+AGG + chr7_3:66661740-66661759 None:intergenic 30.0%
TTGTATTTGTGACAGACATA+TGG + chr7_3:66661870-66661889 None:intergenic 30.0%
TTGAAAATTGTAGTTGGGTA+TGG - chr7_3:66662003-66662022 Msa1118120:intron 30.0%
GCAAAACAATATGATATGCT+TGG - chr7_3:66662064-66662083 Msa1118120:intron 30.0%
AATTGGAAGAAGAAGAATTG+AGG + chr7_3:66662128-66662147 None:intergenic 30.0%
! CTTGTGCAATATTTTCATCT+TGG + chr7_3:66662186-66662205 None:intergenic 30.0%
AGCATTTCAATATGGTCAAT+AGG - chr7_3:66662214-66662233 Msa1118120:intron 30.0%
!! TTTTGGCCTTTAAAGATCTT+TGG - chr7_3:66662491-66662510 Msa1118120:intron 30.0%
! GATAAAGAAAGCTTGATAAC+AGG + chr7_3:66662517-66662536 None:intergenic 30.0%
ATCAAGCTTTCTTTATCTTC+TGG - chr7_3:66662520-66662539 Msa1118120:intron 30.0%
!! ATAGCTTCATGTTAGTCTTA+TGG + chr7_3:66662633-66662652 None:intergenic 30.0%
ATAAGACTAACATGAAGCTA+TGG - chr7_3:66662632-66662651 Msa1118120:intron 30.0%
!!! ACAGTTTTGATTCTTCTAAC+AGG - chr7_3:66662686-66662705 Msa1118120:intron 30.0%
!!! CAGTTTTGATTCTTCTAACA+GGG - chr7_3:66662687-66662706 Msa1118120:intron 30.0%
!! TAGTACTGACAAGAAACTAA+GGG + chr7_3:66663041-66663060 None:intergenic 30.0%
!! TTAGTACTGACAAGAAACTA+AGG + chr7_3:66663042-66663061 None:intergenic 30.0%
TTGGACACTGTTCATTAATA+TGG + chr7_3:66663298-66663317 None:intergenic 30.0%
TTAGTCCTTAAGATTATAGG+TGG - chr7_3:66663414-66663433 Msa1118120:intron 30.0%
ATGGCAGAGAATATTAGATA+AGG + chr7_3:66663449-66663468 None:intergenic 30.0%
ACTGTCAGATCTTAAATCAA+CGG + chr7_3:66663536-66663555 None:intergenic 30.0%
TACCTAATTGGTATTCCTAT+TGG - chr7_3:66663681-66663700 Msa1118120:CDS 30.0%
TATTCTCCATTTGCAAGTTA+AGG - chr7_3:66663719-66663738 Msa1118120:CDS 30.0%
!! GAATTACCTTAACTTGCAAA+TGG + chr7_3:66663728-66663747 None:intergenic 30.0%
! ACCAAAAAGTGTAGTTGAAA+GGG + chr7_3:66663939-66663958 None:intergenic 30.0%
! TACCAAAAAGTGTAGTTGAA+AGG + chr7_3:66663940-66663959 None:intergenic 30.0%
ACAGACAACTAATATGTGTT+GGG - chr7_3:66663995-66664014 Msa1118120:CDS 30.0%
TATCAACATTACATTGGTCA+GGG + chr7_3:66664120-66664139 None:intergenic 30.0%
ATATCAACATTACATTGGTC+AGG + chr7_3:66664121-66664140 None:intergenic 30.0%
!! ATATTGTTGGTGCTGAAATT+GGG - chr7_3:66664301-66664320 Msa1118120:intron 30.0%
! TAATCACATTTAAGACTGAC+TGG - chr7_3:66664391-66664410 Msa1118120:intron 30.0%
! AATCACATTTAAGACTGACT+GGG - chr7_3:66664392-66664411 Msa1118120:intron 30.0%
CCATTTATTAACACGATTTC+TGG + chr7_3:66664534-66664553 None:intergenic 30.0%
CCAGAAATCGTGTTAATAAA+TGG - chr7_3:66664531-66664550 Msa1118120:CDS 30.0%
ATGAATCAAACAACACATCA+AGG - chr7_3:66664570-66664589 Msa1118120:CDS 30.0%
TCAGTGAAGAAAGTCTAATA+AGG - chr7_3:66664631-66664650 Msa1118120:CDS 30.0%
AAAATCATATCTAGCCTCTA+GGG + chr7_3:66664708-66664727 None:intergenic 30.0%
AAAAATCATATCTAGCCTCT+AGG + chr7_3:66664709-66664728 None:intergenic 30.0%
CATTTCCACTTCTTCTACTA+TGG + chr7_3:66661345-66661364 None:intergenic 35.0%
CATAGTAGAAGAAGTGGAAA+TGG - chr7_3:66661343-66661362 Msa1118120:three_prime_UTR 35.0%
AAGAAATTGTGGATAGTAGC+AGG - chr7_3:66661458-66661477 Msa1118120:CDS 35.0%
GAAAACCTATTGAATATGGC+AGG + chr7_3:66661485-66661504 None:intergenic 35.0%
CAATCCTTCATTGGTCATAT+TGG - chr7_3:66661530-66661549 Msa1118120:intron 35.0%
TGAACCAATATGACCAATGA+AGG + chr7_3:66661537-66661556 None:intergenic 35.0%
TATTGCAATTGAGGTAGCAT+CGG + chr7_3:66661828-66661847 None:intergenic 35.0%
GTCTGTCACAAATACAATTG+CGG - chr7_3:66661873-66661892 Msa1118120:intron 35.0%
AGTGCATTAGAAACACTATG+TGG - chr7_3:66662028-66662047 Msa1118120:intron 35.0%
GAGTGTATCTTCAAAGATCA+TGG - chr7_3:66662086-66662105 Msa1118120:intron 35.0%
! AAGTGCCACTAAAATTGGTT+TGG + chr7_3:66662161-66662180 None:intergenic 35.0%
!! AAACCAATTTTAGTGGCACT+TGG - chr7_3:66662160-66662179 Msa1118120:intron 35.0%
GAAAATATTGCACAAGTTGC+TGG - chr7_3:66662190-66662209 Msa1118120:intron 35.0%
!! TTGCTGGAAGCATTTCAATA+TGG - chr7_3:66662206-66662225 Msa1118120:intron 35.0%
TTTGTGCTTGTAGGAACATT+TGG + chr7_3:66662273-66662292 None:intergenic 35.0%
TTGAAATTGAAACTGCTGCA+AGG + chr7_3:66662318-66662337 None:intergenic 35.0%
!! AAGTTCAAGTTTGGCCTTAA+TGG - chr7_3:66662370-66662389 Msa1118120:CDS 35.0%
GCTTATTGGATGCCAAATTT+GGG - chr7_3:66662415-66662434 Msa1118120:intron 35.0%
CTTATTGGATGCCAAATTTG+GGG - chr7_3:66662416-66662435 Msa1118120:intron 35.0%
TGACAAAATGTCCTGATACA+TGG - chr7_3:66662455-66662474 Msa1118120:intron 35.0%
AAAATGTCCTGATACATGGA+AGG - chr7_3:66662459-66662478 Msa1118120:intron 35.0%
AAATGTCCTGATACATGGAA+GGG - chr7_3:66662460-66662479 Msa1118120:intron 35.0%
! ATGGAAGGGTTTCTCATTTT+TGG - chr7_3:66662474-66662493 Msa1118120:intron 35.0%
AACAGGCCAAAGATCTTTAA+AGG + chr7_3:66662500-66662519 None:intergenic 35.0%
!! TATCTTCTGGAGCTATGTTA+TGG - chr7_3:66662533-66662552 Msa1118120:intron 35.0%
!! AGTACTGACAAGAAACTAAG+GGG + chr7_3:66663040-66663059 None:intergenic 35.0%
! AATGGAAAATTTTGCTGCCT+TGG + chr7_3:66663130-66663149 None:intergenic 35.0%
CAAGAATAGCACAAATCCAA+TGG + chr7_3:66663178-66663197 None:intergenic 35.0%
TTGATCTTTCTGTTCCTAAG+AGG - chr7_3:66663195-66663214 Msa1118120:intron 35.0%
! AAATATTTACCAGAGAGCAC+AGG + chr7_3:66663321-66663340 None:intergenic 35.0%
CTTTGCCACCTATAATCTTA+AGG + chr7_3:66663422-66663441 None:intergenic 35.0%
! TTGTGAATAGGTGTTTCTGT+TGG - chr7_3:66663612-66663631 Msa1118120:CDS 35.0%
GTTGTAGCATATGTCAACAT+AGG - chr7_3:66663651-66663670 Msa1118120:CDS 35.0%
ATAGGCTGCTATTACCTAAT+TGG - chr7_3:66663669-66663688 Msa1118120:CDS 35.0%
CTCCAATAGGAATACCAATT+AGG + chr7_3:66663686-66663705 None:intergenic 35.0%
! TGTAAGAGATTAGTCATTGG+TGG + chr7_3:66663883-66663902 None:intergenic 35.0%
ACCAATGACTAATCTCTTAC+AGG - chr7_3:66663882-66663901 Msa1118120:CDS 35.0%
CCAATGACTAATCTCTTACA+GGG - chr7_3:66663883-66663902 Msa1118120:CDS 35.0%
CAATGACTAATCTCTTACAG+GGG - chr7_3:66663884-66663903 Msa1118120:CDS 35.0%
! CCAAAAAGTGTAGTTGAAAG+GGG + chr7_3:66663938-66663957 None:intergenic 35.0%
CACAGACAACTAATATGTGT+TGG - chr7_3:66663994-66664013 Msa1118120:CDS 35.0%
CAGACAACTAATATGTGTTG+GGG - chr7_3:66663996-66664015 Msa1118120:CDS 35.0%
ATCTAACTTACCACCTTTGT+TGG - chr7_3:66664020-66664039 Msa1118120:intron 35.0%
TCTAACTTACCACCTTTGTT+GGG - chr7_3:66664021-66664040 Msa1118120:intron 35.0%
TTACATTGGTCAGGGTAAAT+TGG + chr7_3:66664112-66664131 None:intergenic 35.0%
!! GATATTGTTGGTGCTGAAAT+TGG - chr7_3:66664300-66664319 Msa1118120:intron 35.0%
!!! TGAAATTGGGTCACTTTTGT+AGG - chr7_3:66664314-66664333 Msa1118120:intron 35.0%
!!! GAAATTGGGTCACTTTTGTA+GGG - chr7_3:66664315-66664334 Msa1118120:intron 35.0%
! GTTTGGATTGGAATGTTGTT+TGG - chr7_3:66664339-66664358 Msa1118120:intron 35.0%
!! TGTTAATAAATGGGCTGTAG+TGG - chr7_3:66664541-66664560 Msa1118120:CDS 35.0%
AAATCATATCTAGCCTCTAG+GGG + chr7_3:66664707-66664726 None:intergenic 35.0%
!! AAGGGTTTTGATGCTGCAAA+AGG + chr7_3:66661391-66661410 None:intergenic 40.0%
AAGAAGAGCCACTAAGGAAA+AGG - chr7_3:66661420-66661439 Msa1118120:three_prime_UTR 40.0%
AGAAGAGCCACTAAGGAAAA+GGG - chr7_3:66661421-66661440 Msa1118120:exon 40.0%
GGGAAGAAAGCAAGAAATTG+TGG - chr7_3:66661447-66661466 Msa1118120:CDS 40.0%
GTAGTTGCTCAATCCTTCAT+TGG - chr7_3:66661521-66661540 Msa1118120:intron 40.0%
! AAGGGCATAAGCAGCTAATT+CGG + chr7_3:66661561-66661580 None:intergenic 40.0%
GACTAGGACAGTCATAACAA+GGG + chr7_3:66661579-66661598 None:intergenic 40.0%
TGACTAGGACAGTCATAACA+AGG + chr7_3:66661580-66661599 None:intergenic 40.0%
TTGTTATGACTGTCCTAGTC+AGG - chr7_3:66661579-66661598 Msa1118120:intron 40.0%
TACCATTTGCAAACCTGACT+AGG + chr7_3:66661595-66661614 None:intergenic 40.0%
CAGGTTTGCAAATGGTATTC+TGG - chr7_3:66661598-66661617 Msa1118120:intron 40.0%
ACAAATACAATTGCGGTCGA+TGG - chr7_3:66661880-66661899 Msa1118120:intron 40.0%
ACACTATGTGGACAAGCATA+TGG - chr7_3:66662040-66662059 Msa1118120:intron 40.0%
TGTTCTTGCTTTGTGCTTGT+AGG + chr7_3:66662282-66662301 None:intergenic 40.0%
TGGATGTCATTGCACCATTA+AGG + chr7_3:66662387-66662406 None:intergenic 40.0%
! TGCAATGACATCCATCCTTT+TGG - chr7_3:66662393-66662412 Msa1118120:CDS 40.0%
!!! CATCCATCCTTTTGGCTTAT+TGG - chr7_3:66662401-66662420 Msa1118120:CDS 40.0%
CATCCAATAAGCCAAAAGGA+TGG + chr7_3:66662407-66662426 None:intergenic 40.0%
TTGGCATCCAATAAGCCAAA+AGG + chr7_3:66662411-66662430 None:intergenic 40.0%
GGCTTATTGGATGCCAAATT+TGG - chr7_3:66662414-66662433 Msa1118120:intron 40.0%
AAAACAAGCTGCCCCAAATT+TGG + chr7_3:66662430-66662449 None:intergenic 40.0%
GATTCTTCTAACAGGGAACA+TGG - chr7_3:66662694-66662713 Msa1118120:intron 40.0%
!! CCATATAGCAAGAGCATCAA+TGG + chr7_3:66662735-66662754 None:intergenic 40.0%
! CCATTGATGCTCTTGCTATA+TGG - chr7_3:66662732-66662751 Msa1118120:CDS 40.0%
! TTTTGCAGCCTCAACATCAA+TGG - chr7_3:66662856-66662875 Msa1118120:CDS 40.0%
TTTGCAGCCTCAACATCAAT+GGG - chr7_3:66662857-66662876 Msa1118120:CDS 40.0%
! GTACTGACAAGAAACTAAGG+GGG + chr7_3:66663039-66663058 None:intergenic 40.0%
TGTCGAATGAACTAGGAAGA+GGG - chr7_3:66663100-66663119 Msa1118120:intron 40.0%
! TGTGAGCACTGTTATCACAA+TGG + chr7_3:66663148-66663167 None:intergenic 40.0%
TGTAAGCAAGTCTTCCTCTT+AGG + chr7_3:66663212-66663231 None:intergenic 40.0%
ATTAGCCACATCTGGATTTG+GGG + chr7_3:66663241-66663260 None:intergenic 40.0%
CATTAGCCACATCTGGATTT+GGG + chr7_3:66663242-66663261 None:intergenic 40.0%
ATTTGGATTCTCTGCCGTAA+CGG + chr7_3:66663576-66663595 None:intergenic 40.0%
ATTCCTATTGGAGTTGTGCT+TGG - chr7_3:66663693-66663712 Msa1118120:CDS 40.0%
TTACCAAGCACAACTCCAAT+AGG + chr7_3:66663699-66663718 None:intergenic 40.0%
TCGCACTAATACGATGGTTA+CGG + chr7_3:66663855-66663874 None:intergenic 40.0%
GATGTTTCGCACTAATACGA+TGG + chr7_3:66663861-66663880 None:intergenic 40.0%
! CCCTGTAAGAGATTAGTCAT+TGG + chr7_3:66663886-66663905 None:intergenic 40.0%
ATCTCACCGACCATATTTAG+TGG - chr7_3:66663907-66663926 Msa1118120:CDS 40.0%
TCTCACCGACCATATTTAGT+GGG - chr7_3:66663908-66663927 Msa1118120:CDS 40.0%
GAGATCCCACTAAATATGGT+CGG + chr7_3:66663916-66663935 None:intergenic 40.0%
! CCCCTTTCAACTACACTTTT+TGG - chr7_3:66663935-66663954 Msa1118120:CDS 40.0%
CTAACTTACCACCTTTGTTG+GGG - chr7_3:66664022-66664041 Msa1118120:intron 40.0%
TGGTCAGGGTAAATTGGATT+TGG + chr7_3:66664106-66664125 None:intergenic 40.0%
!! CTTTTGTAGGGTGTTTGGAT+TGG - chr7_3:66664327-66664346 Msa1118120:intron 40.0%
TCTAGTCAAAATACCCCTAG+AGG - chr7_3:66664691-66664710 Msa1118120:five_prime_UTR 40.0%
CAGCTCCATAGTAGAAGAAG+TGG - chr7_3:66661337-66661356 Msa1118120:three_prime_UTR 45.0%
GAAGAAGTGGAAATGGGATC+AGG - chr7_3:66661350-66661369 Msa1118120:three_prime_UTR 45.0%
AGCCACTAAGGAAAAGGGTA+TGG - chr7_3:66661426-66661445 Msa1118120:exon 45.0%
! GCCACTAAGGAAAAGGGTAT+GGG - chr7_3:66661427-66661446 Msa1118120:exon 45.0%
! TCCCATACCCTTTTCCTTAG+TGG + chr7_3:66661431-66661450 None:intergenic 45.0%
GTCCTAGTCAGGTTTGCAAA+TGG - chr7_3:66661590-66661609 Msa1118120:intron 45.0%
! TGGCCAAGTGCCACTAAAAT+TGG + chr7_3:66662166-66662185 None:intergenic 45.0%
GAGAAACCCTTCCATGTATC+AGG + chr7_3:66662469-66662488 None:intergenic 45.0%
TTTCCCACCCATTGATGTTG+AGG + chr7_3:66662867-66662886 None:intergenic 45.0%
TGGGAAATGATGATAGCCCT+TGG - chr7_3:66662880-66662899 Msa1118120:CDS 45.0%
AGCTGCAGCAAAGAAACCAA+GGG + chr7_3:66662899-66662918 None:intergenic 45.0%
TAGCTGCAGCAAAGAAACCA+AGG + chr7_3:66662900-66662919 None:intergenic 45.0%
TTGGTTTCTTTGCTGCAGCT+AGG - chr7_3:66662899-66662918 Msa1118120:CDS 45.0%
!! GTTAGGGTGTCGAATGAACT+AGG - chr7_3:66663093-66663112 Msa1118120:intron 45.0%
GTGTCGAATGAACTAGGAAG+AGG - chr7_3:66663099-66663118 Msa1118120:intron 45.0%
GTGCTCACATCATTTGCCAT+TGG - chr7_3:66663159-66663178 Msa1118120:CDS 45.0%
TTTCACCCCAAATCCAGATG+TGG - chr7_3:66663233-66663252 Msa1118120:intron 45.0%
GCATTAGCCACATCTGGATT+TGG + chr7_3:66663243-66663262 None:intergenic 45.0%
! ATTTACCAGAGAGCACAGGT+TGG + chr7_3:66663317-66663336 None:intergenic 45.0%
! ATAGGTGTTTCTGTTGGAGC+TGG - chr7_3:66663618-66663637 Msa1118120:CDS 45.0%
AGGGGAGATCCCACTAAATA+TGG + chr7_3:66663920-66663939 None:intergenic 45.0%
!!! GGTCACTTTTGTAGGGTGTT+TGG - chr7_3:66664322-66664341 Msa1118120:intron 45.0%
GCAGCATCAAAACCCTTCAG+AGG - chr7_3:66661394-66661413 Msa1118120:three_prime_UTR 50.0%
GCATCAAAACCCTTCAGAGG+AGG - chr7_3:66661397-66661416 Msa1118120:three_prime_UTR 50.0%
GCTCTTCTTCCTCCTCTGAA+GGG + chr7_3:66661409-66661428 None:intergenic 50.0%
AGGAGGAAGAAGAGCCACTA+AGG - chr7_3:66661414-66661433 Msa1118120:three_prime_UTR 50.0%
CAGGCCCTGCCATATTCAAT+AGG - chr7_3:66661477-66661496 Msa1118120:CDS 50.0%
TTGCGATCGAGTGCAGTGTA+TGG - chr7_3:66661757-66661776 Msa1118120:intron 50.0%
CAGCCTCAACATCAATGGGT+GGG - chr7_3:66662861-66662880 Msa1118120:CDS 50.0%
CTGACAAGAAACTAAGGGGG+TGG + chr7_3:66663036-66663055 None:intergenic 50.0%
ACTAGGAAGAGGGAGTTCCA+AGG - chr7_3:66663110-66663129 Msa1118120:intron 50.0%
CCAACTGCATTAGCCACATC+TGG + chr7_3:66663249-66663268 None:intergenic 50.0%
CCAGATGTGGCTAATGCAGT+TGG - chr7_3:66663246-66663265 Msa1118120:intron 50.0%
TAACGGCGAGACGGTCTATT+TGG + chr7_3:66663559-66663578 None:intergenic 50.0%
AATAGACCGTCTCGCCGTTA+CGG - chr7_3:66663559-66663578 Msa1118120:CDS 50.0%
GATAGGAGAGACCCCAACAA+AGG + chr7_3:66664036-66664055 None:intergenic 50.0%
TAAGACTGACTGGGACAAGC+AGG - chr7_3:66664401-66664420 Msa1118120:intron 50.0%
GGCTCTTCTTCCTCCTCTGA+AGG + chr7_3:66661410-66661429 None:intergenic 55.0%
GCAGCCTCAACATCAATGGG+TGG - chr7_3:66662860-66662879 Msa1118120:CDS 55.0%
AGTGTCCAACCTGTGCTCTC+TGG - chr7_3:66663309-66663328 Msa1118120:CDS 55.0%
! GTGTTTCTGTTGGAGCTGGC+TGG - chr7_3:66663622-66663641 Msa1118120:CDS 55.0%
AGGAGAGACCCCAACAAAGG+TGG + chr7_3:66664033-66664052 None:intergenic 55.0%
TCTCTGCCGTAACGGCGAGA+CGG + chr7_3:66663568-66663587 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 66661252 66664792 66661252 ID=Msa1118120;Name=Msa1118120
chr7_3 mRNA 66661252 66664792 66661252 ID=Msa1118120-mRNA-1;Parent=Msa1118120;Name=Msa1118120-mRNA-1;_AED=0.39;_eAED=0.39;_QI=110|1|1|1|1|1|8|188|489
chr7_3 exon 66661252 66661523 66661252 ID=Msa1118120-mRNA-1:exon:16221;Parent=Msa1118120-mRNA-1
chr7_3 exon 66661622 66661708 66661622 ID=Msa1118120-mRNA-1:exon:16220;Parent=Msa1118120-mRNA-1
chr7_3 exon 66662304 66662422 66662304 ID=Msa1118120-mRNA-1:exon:16219;Parent=Msa1118120-mRNA-1
chr7_3 exon 66662714 66662952 66662714 ID=Msa1118120-mRNA-1:exon:16218;Parent=Msa1118120-mRNA-1
chr7_3 exon 66663124 66663180 66663124 ID=Msa1118120-mRNA-1:exon:16217;Parent=Msa1118120-mRNA-1
chr7_3 exon 66663291 66663377 66663291 ID=Msa1118120-mRNA-1:exon:16216;Parent=Msa1118120-mRNA-1
chr7_3 exon 66663490 66664028 66663490 ID=Msa1118120-mRNA-1:exon:16215;Parent=Msa1118120-mRNA-1
chr7_3 exon 66664425 66664792 66664425 ID=Msa1118120-mRNA-1:exon:16214;Parent=Msa1118120-mRNA-1
chr7_3 five_prime_UTR 66664683 66664792 66664683 ID=Msa1118120-mRNA-1:five_prime_utr;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66664425 66664682 66664425 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66663490 66664028 66663490 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66663291 66663377 66663291 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66663124 66663180 66663124 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66662714 66662952 66662714 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66662304 66662422 66662304 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66661622 66661708 66661622 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 CDS 66661440 66661523 66661440 ID=Msa1118120-mRNA-1:cds;Parent=Msa1118120-mRNA-1
chr7_3 three_prime_UTR 66661252 66661439 66661252 ID=Msa1118120-mRNA-1:three_prime_utr;Parent=Msa1118120-mRNA-1
Gene Sequence

>Msa1118120

ATGGGATCAGGAGAGATAAACAAGAACCTTTTGCAGCATCAAAACCCTTCAGAGGAGGAAGAAGAGCCACTAAGGAAAAGGGTATGGGAAGAAAGCAAGAAATTGTGGATAGTAGCAGGCCCTGCCATATTCAATAGGTTTTCAACATTTGGTATCATGGTAGTTGCTCAATCCTTCATTGGTCATATTGGTTCAACCGAATTAGCTGCTTATGCCCTTGTTATGACTGTCCTAGTCAGGTTTGCAAATGGTATTCTGTTGGGTATGGCAAGTGCATTAGAAACACTATGTGGACAAGCATATGGAGCAAAACAATATGATATGCTTGGAGTGTATCTTCAAAGATCATGGATAGTTATATTCTTAACCTCAATTCTTCTTCTTCCAATTTACATTTTTACCAAACCAATTTTAGTGGCACTTGGCCAAGATGAAAATATTGCACAAGTTGCTGGAAGCATTTCAATATGGTCAATAGGTATAGTATTTGCTTTCTTTGCATCATTCACTTGCCAAATGTTCCTACAAGCACAAAGCAAGAACAAGATTATTGCTTACCTTGCAGCAGTTTCAATTTCAATACATGTTTTTATGTCATGGCTTTTAACTGTTAAGTTCAAGTTTGGCCTTAATGGTGCAATGACATCCATCCTTTTGGCTTATTGGATGCCAAATTTGGGGCAGCTTGTTTTTATCATGACAAAATGTCCTGATACATGGAAGGGTTTCTCATTTTTGGCCTTTAAAGATCTTTGGCCTGTTATCAAGCTTTCTTTATCTTCTGGAGCTATGTTATGTCTTGAAATATGGTACAACACAGTTTTGATTCTTCTAACAGGGAACATGGAAAATGCTGAGATTTCCATTGATGCTCTTGCTATATGCCTCAACATCAATGGGTGGGAAATGATGATAGCCCTTGGTTTCTTTGCTGCAGCTAGTGTTAGGGTGTCGAATGAACTAGGAAGAGGGAGTTCCAAGGCAGCAAAATTTTCCATTGTGATAACAGTGCTCACATCATTTGCCATTGGATTTGTGCTATTCTTGATCTTTCTGTTCCTAAGAGGAAGACTTGCTTACATTTTCACCCCAAATCCAGATGTGGCTAATGCAGTTGGAGATCTATCACCTTTGTTATCATTCTCCATATTAATGAACAGTGTCCAACCTGTGCTCTCTGGTGTTTCTGTTGGAGCTGGCTGGCAAAGTGTTGTAGCATATGTCAACATAGGCTGCTATTACCTAATTGGTATTCCTATTGGAGTTGTGCTTGGTAATATTCTCCATTTGCAAGTTAAGGGTGTTTGGATTGGAATGTTGTTTGGAACTTTTGTTCAAACCATAATGCTTATGATAATCACATTTAAGACTGACTGGGACAAGCAGGTTGAGATTGCCAGAAATCGTGTTAATAAATGGGCTGTAGTGGAGAATGATGAATCAAACAACACATCAAGGATATCCAACTAA
Protein sequence

>Msa1118120

MGSGEINKNLLQHQNPSEEEEEPLRKRVWEESKKLWIVAGPAIFNRFSTFGIMVVAQSFIGHIGSTELAAYALVMTVLVRFANGILLGMASALETLCGQAYGAKQYDMLGVYLQRSWIVIFLTSILLLPIYIFTKPILVALGQDENIAQVAGSISIWSIGIVFAFFASFTCQMFLQAQSKNKIIAYLAAVSISIHVFMSWLLTVKFKFGLNGAMTSILLAYWMPNLGQLVFIMTKCPDTWKGFSFLAFKDLWPVIKLSLSSGAMLCLEIWYNTVLILLTGNMENAEISIDALAICLNINGWEMMIALGFFAAASVRVSNELGRGSSKAAKFSIVITVLTSFAIGFVLFLIFLFLRGRLAYIFTPNPDVANAVGDLSPLLSFSILMNSVQPVLSGVSVGAGWQSVVAYVNIGCYYLIGIPIGVVLGNILHLQVKGVWIGMLFGTFVQTIMLMIITFKTDWDKQVEIARNRVNKWAVVENDESNNTSRISN*