AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1156440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156440 MtrunA17_Chr7g0249831 99.115 113 1 0 1 113 1 113 7.09e-82 234
Msa1156440 MtrunA17_Chr5g0396151 88.496 113 13 0 1 113 1 113 1.71e-73 213
Msa1156440 MtrunA17_Chr7g0249821 70.370 108 31 1 1 108 1 107 1.24e-51 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156440 AT4G10040.1 91.071 112 10 0 1 112 1 112 4.58e-75 218
Msa1156440 AT1G22840.1 89.286 112 12 0 1 112 1 112 2.16e-73 213
Msa1156440 AT1G22840.2 88.095 84 10 0 1 84 1 84 4.72e-52 159

Find 77 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAATTTATTTATCTTAA+AGG 0.085752 7_4:-44717160 None:intergenic
TTTCATTCATGCTAATATTT+TGG 0.129707 7_4:-44717102 None:intergenic
CTCGCCGGCGGCGGGGTTTC+CGG 0.164134 7_4:-44717509 None:intergenic
GGAAAGCTACACCGCCATTT+TGG 0.196265 7_4:-44717348 None:intergenic
GTAGCTTTCCTATAACTTTC+GGG 0.235809 7_4:+44717361 Msa1156440:five_prime_UTR
ATGTATGACCCGAAAGTTAT+AGG 0.236852 7_4:-44717369 None:intergenic
TCGCCGGCGGCGGGGTTTCC+GGG 0.240370 7_4:-44717508 None:intergenic
TGTAGCTTTCCTATAACTTT+CGG 0.256249 7_4:+44717360 Msa1156440:five_prime_UTR
CCCAATCTGAATGGTTTGTT+TGG 0.305862 7_4:+44719005 Msa1156440:CDS
ATCAATGAGGTGTACCTTCT+TGG 0.316265 7_4:-44719129 None:intergenic
CTTCTTGAGGGTGCGTATAT+TGG 0.326131 7_4:+44719632 Msa1156440:three_prime_UTR
GGTTTGTTTGGAAGGCAATC+TGG 0.326375 7_4:+44719017 Msa1156440:CDS
GTACGAAAATTTAAAATTCC+AGG 0.329136 7_4:-44719516 None:intergenic
AAGATCTTCTCGCCGGCGGC+GGG 0.329325 7_4:-44717517 None:intergenic
TGCATTGCATTGCTTCTTGA+GGG 0.332056 7_4:+44719620 Msa1156440:three_prime_UTR
TCAATGAGGTGTACCTTCTT+GGG 0.342267 7_4:-44719128 None:intergenic
CTCCAGTTTGTGAAATTAAG+AGG 0.347757 7_4:-44719827 None:intergenic
TTCCTCTTAATTTCACAAAC+TGG 0.357425 7_4:+44719825 Msa1156440:three_prime_UTR
GCTAACAAAATCGAATGAAA+TGG 0.359165 7_4:+44717232 Msa1156440:five_prime_UTR
GTGATTTCGGTGGCCCAAAA+TGG 0.367212 7_4:+44717334 Msa1156440:five_prime_UTR
GACGCCATTTGAGAAAGTTC+TGG 0.371487 7_4:-44717469 None:intergenic
CAAGAACAAGGCTGTGGATT+GGG 0.392913 7_4:+44719073 Msa1156440:CDS
GTTTCCAGAACTTTCTCAAA+TGG 0.417788 7_4:+44717465 Msa1156440:exon
GAAGATCTTCTCGCCGGCGG+CGG 0.418146 7_4:-44717518 None:intergenic
TCCAAACAAACCATTCAGAT+TGG 0.424075 7_4:-44719006 None:intergenic
ATCTGAATGGTTTGTTTGGA+AGG 0.441498 7_4:+44719009 Msa1156440:CDS
AGGCAATCTGGGACAACTCC+TGG 0.447271 7_4:+44719029 Msa1156440:CDS
AAACTGGAGTTCTTTGTATG+TGG 0.451950 7_4:+44719841 Msa1156440:three_prime_UTR
TTCTTTGTATGTGGATTGAG+AGG 0.456920 7_4:+44719850 Msa1156440:three_prime_UTR
ATCTTAATGCAGTACATCCC+AGG 0.460110 7_4:+44719369 Msa1156440:intron
TTGCATTGCATTGCTTCTTG+AGG 0.464608 7_4:+44719619 Msa1156440:three_prime_UTR
CTAACAAAATCGAATGAAAT+GGG 0.464744 7_4:+44717233 Msa1156440:five_prime_UTR
GTTTGTTTGGAAGGCAATCT+GGG 0.482470 7_4:+44719018 Msa1156440:CDS
CCAAACAAACCATTCAGATT+GGG 0.484079 7_4:-44719005 None:intergenic
AATTTATTTATCTTAAAGGA+TGG 0.485319 7_4:-44717156 None:intergenic
AGAATAGTAAACCTTGTTTG+TGG 0.491881 7_4:-44717585 None:intergenic
ACCTTAGGACCCAATCTGAA+TGG 0.493293 7_4:+44718996 Msa1156440:intron
GCTTTATTTGCCAAGAAAAG+TGG 0.493817 7_4:-44719561 None:intergenic
TCCTTCGATGAAGCTCCTCC+CGG 0.495242 7_4:+44717490 Msa1156440:CDS
ACAAGAACAAGGCTGTGGAT+TGG 0.503827 7_4:+44719072 Msa1156440:CDS
AAGAAGCAATGCAATGCAAA+AGG 0.507620 7_4:-44719615 None:intergenic
TAGTAAACCTTGTTTGTGGC+CGG 0.509984 7_4:-44717581 None:intergenic
GTGGCCGGCGCCTTTGTCGA+CGG 0.510944 7_4:-44717566 None:intergenic
GTGTTGCGAAAGAATTGTAT+TGG 0.519266 7_4:-44717435 None:intergenic
GGTGTGGCACTGAGCGCACT+TGG 0.520968 7_4:-44717545 None:intergenic
CCTTTAAGATAAATAAATTG+AGG 0.523855 7_4:+44717160 Msa1156440:five_prime_UTR
GCTGCAGAGTAGGAATATCC+AGG 0.542193 7_4:-44719047 None:intergenic
ATGTGGATTGAGAGGAGGAT+AGG 0.545231 7_4:+44719858 Msa1156440:three_prime_UTR
TCTTGAGGCTTCTTAAGACC+AGG 0.550991 7_4:-44719408 None:intergenic
CCTCCCGGAAACCCCGCCGC+CGG 0.551439 7_4:+44717505 Msa1156440:CDS
TGAGATTCAGTTTATTGACC+TGG 0.552995 7_4:+44719900 Msa1156440:three_prime_UTR
AGATCTTCTCGCCGGCGGCG+GGG 0.553653 7_4:-44717516 None:intergenic
GGATAGGTTGAATTTCAAGT+TGG 0.556911 7_4:+44719874 Msa1156440:three_prime_UTR
GTTCTTGTTAGCTGCAGAGT+AGG 0.557927 7_4:-44719057 None:intergenic
AGTAAACAAAATCACAAGAC+TGG 0.564575 7_4:-44719763 None:intergenic
CAATGAGGTGTACCTTCTTG+GGG 0.580286 7_4:-44719127 None:intergenic
AGACTAATATTTGAACACAT+TGG 0.581329 7_4:+44719657 Msa1156440:three_prime_UTR
CAGTGCCACACCGTCGACAA+AGG 0.583975 7_4:+44717556 Msa1156440:CDS
TTTGTATGTGGATTGAGAGG+AGG 0.585787 7_4:+44719853 Msa1156440:three_prime_UTR
ATAAGATCAGCACGTTCTTG+AGG 0.588809 7_4:-44719423 None:intergenic
GTACATCCCAGGGACGAAGA+TGG 0.591227 7_4:+44719380 Msa1156440:intron
TTTGAAGATCTTCTCGCCGG+CGG 0.594334 7_4:-44717521 None:intergenic
TCCGGGAGGAGCTTCATCGA+AGG 0.600967 7_4:-44717491 None:intergenic
AAAGGCGCCGGCCACAAACA+AGG 0.601379 7_4:+44717574 Msa1156440:CDS
ACTGTTTGAGCACAAGCACC+AGG 0.602572 7_4:-44719918 None:intergenic
CTCTGCAGCTAACAAGAACA+AGG 0.605687 7_4:+44719061 Msa1156440:CDS
AGCTAACAAGAACAAGGCTG+TGG 0.608122 7_4:+44719067 Msa1156440:CDS
TCTTAATGCAGTACATCCCA+GGG 0.608747 7_4:+44719370 Msa1156440:intron
CACACCGTCGACAAAGGCGC+CGG 0.616193 7_4:+44717562 Msa1156440:CDS
GTTCAGCAAGTAATCGTACA+AGG 0.624998 7_4:-44719105 None:intergenic
TTGAGAAAGTTCTGGAAACG+AGG 0.625386 7_4:-44717461 None:intergenic
GAACAAGGCTGTGGATTGGG+AGG 0.635538 7_4:+44719076 Msa1156440:CDS
TTACTTGCTGAACCCCAAGA+AGG 0.638569 7_4:+44719115 Msa1156440:CDS
CGGCGCCTTTGTCGACGGTG+TGG 0.644445 7_4:-44717561 None:intergenic
CCGGCGGCGGGGTTTCCGGG+AGG 0.653806 7_4:-44717505 None:intergenic
ATTTCGGTGGCCCAAAATGG+CGG 0.654704 7_4:+44717337 Msa1156440:five_prime_UTR
TCGTTGACACGAACATTACG+CGG 0.669349 7_4:-44717204 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATTTACATAGTTT+AGG + chr7_4:44718279-44718298 Msa1156440:intron 10.0%
!! TATTATATTTACATAGTTTA+GGG + chr7_4:44718280-44718299 Msa1156440:intron 10.0%
!!! TTTTTTATATATTTATTGCA+AGG + chr7_4:44718559-44718578 Msa1156440:intron 10.0%
!! AATTTATTTATCTTAAAGGA+TGG - chr7_4:44717159-44717178 None:intergenic 15.0%
!! AAAAAATGAAAAAGTGATTT+CGG + chr7_4:44717321-44717340 Msa1156440:five_prime_UTR 15.0%
!! TAAATATAATAAGTAATCTG+AGG - chr7_4:44718271-44718290 None:intergenic 15.0%
!! ACAATTATTGAAATAACATT+GGG - chr7_4:44718641-44718660 None:intergenic 15.0%
!! TACAATTATTGAAATAACAT+TGG - chr7_4:44718642-44718661 None:intergenic 15.0%
!! CATATGTGAAAAATATTAAA+CGG - chr7_4:44718814-44718833 None:intergenic 15.0%
!! ATAAAAATGTCTATATCAAA+TGG + chr7_4:44719181-44719200 Msa1156440:intron 15.0%
!!! TAAATTCTATTATTTTGAAG+TGG - chr7_4:44719466-44719485 None:intergenic 15.0%
!!! TTTCATTCATGCTAATATTT+TGG - chr7_4:44717105-44717124 None:intergenic 20.0%
!! CCTCAATTTATTTATCTTAA+AGG - chr7_4:44717163-44717182 None:intergenic 20.0%
!!! CCTTTAAGATAAATAAATTG+AGG + chr7_4:44717160-44717179 Msa1156440:five_prime_UTR 20.0%
!! ATTTGATCTCTAAATATCTT+GGG + chr7_4:44718038-44718057 Msa1156440:intron 20.0%
!!! AAATGAATAAACCATTGATT+TGG - chr7_4:44718307-44718326 None:intergenic 20.0%
!!! ATGAAAATGTTTTGTAAACT+TGG + chr7_4:44718364-44718383 Msa1156440:intron 20.0%
!! CAATTATTGAAATAACATTG+GGG - chr7_4:44718640-44718659 None:intergenic 20.0%
!! TTACAAAATGACATTATTCA+AGG - chr7_4:44718938-44718957 None:intergenic 20.0%
!! GTTATGCTGTTAATTTATTA+TGG + chr7_4:44719277-44719296 Msa1156440:intron 20.0%
! CTAACAAAATCGAATGAAAT+GGG + chr7_4:44717233-44717252 Msa1156440:five_prime_UTR 25.0%
! AAAAAACGAAAAAACATCAC+AGG - chr7_4:44717652-44717671 None:intergenic 25.0%
! TAAGAATCTATTGTATTGTG+TGG + chr7_4:44717768-44717787 Msa1156440:intron 25.0%
!! AATACTTATGTAAGTTGGAT+CGG - chr7_4:44717889-44717908 None:intergenic 25.0%
! TCCAACTTACATAAGTATTA+AGG + chr7_4:44717890-44717909 Msa1156440:intron 25.0%
! ACCTTAATACTTATGTAAGT+TGG - chr7_4:44717894-44717913 None:intergenic 25.0%
! AATCCGTAAACTATAGAAAT+CGG + chr7_4:44717994-44718013 Msa1156440:intron 25.0%
! CATTTGATCTCTAAATATCT+TGG + chr7_4:44718037-44718056 Msa1156440:intron 25.0%
! TTGTGAAAAAAACCAAAAGA+TGG - chr7_4:44718476-44718495 None:intergenic 25.0%
!!! ATCTTTTGGTTTTTTTCACA+AGG + chr7_4:44718475-44718494 Msa1156440:intron 25.0%
! TGAATGAATTGTGATTAGAA+AGG + chr7_4:44718693-44718712 Msa1156440:intron 25.0%
!! CATTTAGTGTTTTCTACAAT+TGG + chr7_4:44719248-44719267 Msa1156440:intron 25.0%
!!! ATTATGGTTATGTTGATGAT+AGG + chr7_4:44719293-44719312 Msa1156440:intron 25.0%
!! GTACGAAAATTTAAAATTCC+AGG - chr7_4:44719519-44719538 None:intergenic 25.0%
!! TTTTCGTACATTTGATTATG+TGG + chr7_4:44719530-44719549 Msa1156440:three_prime_UTR 25.0%
! AGACTAATATTTGAACACAT+TGG + chr7_4:44719657-44719676 Msa1156440:three_prime_UTR 25.0%
GCTAACAAAATCGAATGAAA+TGG + chr7_4:44717232-44717251 Msa1156440:five_prime_UTR 30.0%
AAATGAAAAAGTGATTTCGG+TGG + chr7_4:44717324-44717343 Msa1156440:five_prime_UTR 30.0%
TGTAGCTTTCCTATAACTTT+CGG + chr7_4:44717360-44717379 Msa1156440:five_prime_UTR 30.0%
! AGAGAATATGCTATGCTTTT+TGG - chr7_4:44717400-44717419 None:intergenic 30.0%
! AGAATAGTAAACCTTGTTTG+TGG - chr7_4:44717588-44717607 None:intergenic 30.0%
GAAAAAACTTAACAGCGATT+AGG - chr7_4:44717706-44717725 None:intergenic 30.0%
TGATTGTTCAAGATCTTGTT+AGG + chr7_4:44717742-44717761 Msa1156440:intron 30.0%
GATTGTTCAAGATCTTGTTA+GGG + chr7_4:44717743-44717762 Msa1156440:intron 30.0%
TGTTGAAGCTTGAAATGTTT+AGG + chr7_4:44717800-44717819 Msa1156440:intron 30.0%
ACTCCGATTTCTATAGTTTA+CGG - chr7_4:44718000-44718019 None:intergenic 30.0%
ACTCAGCTAAAAAAACAAGA+AGG - chr7_4:44718753-44718772 None:intergenic 30.0%
!!! TCTTGTTTTTTTAGCTGAGT+CGG + chr7_4:44718753-44718772 Msa1156440:intron 30.0%
GTTGAATGAACAAAACTCAA+GGG + chr7_4:44718775-44718794 Msa1156440:intron 30.0%
AATGACATTATTCAAGGAGA+GGG - chr7_4:44718932-44718951 None:intergenic 30.0%
AAATGACATTATTCAAGGAG+AGG - chr7_4:44718933-44718952 None:intergenic 30.0%
! TAGTGTTTTCTACAATTGGT+AGG + chr7_4:44719252-44719271 Msa1156440:intron 30.0%
ACGCATATCAGTAAAATACT+TGG - chr7_4:44719341-44719360 None:intergenic 30.0%
AGTAAACAAAATCACAAGAC+TGG - chr7_4:44719766-44719785 None:intergenic 30.0%
TTCCTCTTAATTTCACAAAC+TGG + chr7_4:44719825-44719844 Msa1156440:three_prime_UTR 30.0%
GTAGCTTTCCTATAACTTTC+GGG + chr7_4:44717361-44717380 Msa1156440:five_prime_UTR 35.0%
ATGTATGACCCGAAAGTTAT+AGG - chr7_4:44717372-44717391 None:intergenic 35.0%
GTGTTGCGAAAGAATTGTAT+TGG - chr7_4:44717438-44717457 None:intergenic 35.0%
GTTTCCAGAACTTTCTCAAA+TGG + chr7_4:44717465-44717484 Msa1156440:exon 35.0%
!! CGTAGATCTGAGAGTATTAA+GGG - chr7_4:44717626-44717645 None:intergenic 35.0%
TTGGTTCCCTAATCTCTTAA+TGG + chr7_4:44718074-44718093 Msa1156440:intron 35.0%
TCTCAGCCATTAAGAGATTA+GGG - chr7_4:44718083-44718102 None:intergenic 35.0%
ATCTCAGCCATTAAGAGATT+AGG - chr7_4:44718084-44718103 None:intergenic 35.0%
TTAAACAGCGTTGTCAATCT+TGG + chr7_4:44718103-44718122 Msa1156440:intron 35.0%
TTGTCAAAAAGCCTCTATTG+CGG - chr7_4:44718174-44718193 None:intergenic 35.0%
CAACACTTTACACTGAATAG+TGG + chr7_4:44718191-44718210 Msa1156440:intron 35.0%
TAGTTTAGGGACCAAATCAA+TGG + chr7_4:44718293-44718312 Msa1156440:intron 35.0%
CTTGGTATGTTCAGAATGTT+TGG + chr7_4:44718382-44718401 Msa1156440:intron 35.0%
!!! GTTTTGTTTGAGAGATGAGA+GGG + chr7_4:44718443-44718462 Msa1156440:intron 35.0%
GAAATAACATTGGGGTGATA+TGG - chr7_4:44718632-44718651 None:intergenic 35.0%
GGTTGAATGAACAAAACTCA+AGG + chr7_4:44718774-44718793 Msa1156440:intron 35.0%
CCAAACAAACCATTCAGATT+GGG - chr7_4:44719008-44719027 None:intergenic 35.0%
TCCAAACAAACCATTCAGAT+TGG - chr7_4:44719009-44719028 None:intergenic 35.0%
!! ATCTGAATGGTTTGTTTGGA+AGG + chr7_4:44719009-44719028 Msa1156440:CDS 35.0%
! TGTTTTCTACAATTGGTAGG+AGG + chr7_4:44719255-44719274 Msa1156440:intron 35.0%
TTGGTTGAAACAGACTTTCA+AGG - chr7_4:44719322-44719341 None:intergenic 35.0%
!! GCTTTATTTGCCAAGAAAAG+TGG - chr7_4:44719564-44719583 None:intergenic 35.0%
AAGAAGCAATGCAATGCAAA+AGG - chr7_4:44719618-44719637 None:intergenic 35.0%
CTCCAGTTTGTGAAATTAAG+AGG - chr7_4:44719830-44719849 None:intergenic 35.0%
AAACTGGAGTTCTTTGTATG+TGG + chr7_4:44719841-44719860 Msa1156440:three_prime_UTR 35.0%
! TTCTTTGTATGTGGATTGAG+AGG + chr7_4:44719850-44719869 Msa1156440:three_prime_UTR 35.0%
! GGATAGGTTGAATTTCAAGT+TGG + chr7_4:44719874-44719893 Msa1156440:three_prime_UTR 35.0%
TGAGATTCAGTTTATTGACC+TGG + chr7_4:44719900-44719919 Msa1156440:three_prime_UTR 35.0%
!! TTGAGAAAGTTCTGGAAACG+AGG - chr7_4:44717464-44717483 None:intergenic 40.0%
! TAGTAAACCTTGTTTGTGGC+CGG - chr7_4:44717584-44717603 None:intergenic 40.0%
!! GCGTAGATCTGAGAGTATTA+AGG - chr7_4:44717627-44717646 None:intergenic 40.0%
GGATCGGACATAACTGAAAT+TGG - chr7_4:44717873-44717892 None:intergenic 40.0%
! CTTGGGCTTAAATGCACTTT+TGG + chr7_4:44718055-44718074 Msa1156440:intron 40.0%
! CAGAATGTTTGGTTCAAACC+AGG + chr7_4:44718393-44718412 Msa1156440:intron 40.0%
!!! CGTTTTGTTTGAGAGATGAG+AGG + chr7_4:44718442-44718461 Msa1156440:intron 40.0%
!!! GGTTTTTTTCACAAGGAGAG+AGG + chr7_4:44718482-44718501 Msa1156440:intron 40.0%
! CTTTATTTGTTGCCGACCTT+AGG + chr7_4:44718981-44719000 Msa1156440:intron 40.0%
!! CCCAATCTGAATGGTTTGTT+TGG + chr7_4:44719005-44719024 Msa1156440:CDS 40.0%
GTTTGTTTGGAAGGCAATCT+GGG + chr7_4:44719018-44719037 Msa1156440:CDS 40.0%
GTTCAGCAAGTAATCGTACA+AGG - chr7_4:44719108-44719127 None:intergenic 40.0%
TCAATGAGGTGTACCTTCTT+GGG - chr7_4:44719131-44719150 None:intergenic 40.0%
ATCAATGAGGTGTACCTTCT+TGG - chr7_4:44719132-44719151 None:intergenic 40.0%
! AGAAGGTACACCTCATTGAT+TGG + chr7_4:44719132-44719151 Msa1156440:intron 40.0%
CACAGAAATGCCAATCAATG+AGG - chr7_4:44719145-44719164 None:intergenic 40.0%
ATCTTAATGCAGTACATCCC+AGG + chr7_4:44719369-44719388 Msa1156440:intron 40.0%
TCTTAATGCAGTACATCCCA+GGG + chr7_4:44719370-44719389 Msa1156440:intron 40.0%
ATAAGATCAGCACGTTCTTG+AGG - chr7_4:44719426-44719445 None:intergenic 40.0%
! TGCAGTATGCTTTTCAAACC+TGG + chr7_4:44719498-44719517 Msa1156440:three_prime_UTR 40.0%
TTGCATTGCATTGCTTCTTG+AGG + chr7_4:44719619-44719638 Msa1156440:three_prime_UTR 40.0%
TGCATTGCATTGCTTCTTGA+GGG + chr7_4:44719620-44719639 Msa1156440:three_prime_UTR 40.0%
! TTTGTATGTGGATTGAGAGG+AGG + chr7_4:44719853-44719872 Msa1156440:three_prime_UTR 40.0%
TCGTTGACACGAACATTACG+CGG - chr7_4:44717207-44717226 None:intergenic 45.0%
!! GAAAGCTACACCGCCATTTT+GGG - chr7_4:44717350-44717369 None:intergenic 45.0%
GACGCCATTTGAGAAAGTTC+TGG - chr7_4:44717472-44717491 None:intergenic 45.0%
!!! GGTTTTGAAGATCTTCTCGC+CGG - chr7_4:44717527-44717546 None:intergenic 45.0%
! TGTTTGGTTCAAACCAGGCA+AGG + chr7_4:44718398-44718417 Msa1156440:intron 45.0%
! GAGGGCAAACAACCATCTTT+TGG + chr7_4:44718461-44718480 Msa1156440:intron 45.0%
! ATTATTCAAGGAGAGGGCAC+AGG - chr7_4:44718926-44718945 None:intergenic 45.0%
TTCAGATTGGGTCCTAAGGT+CGG - chr7_4:44718996-44719015 None:intergenic 45.0%
ACCTTAGGACCCAATCTGAA+TGG + chr7_4:44718996-44719015 Msa1156440:intron 45.0%
ACCATTCAGATTGGGTCCTA+AGG - chr7_4:44719000-44719019 None:intergenic 45.0%
! GGTTTGTTTGGAAGGCAATC+TGG + chr7_4:44719017-44719036 Msa1156440:CDS 45.0%
GTTCTTGTTAGCTGCAGAGT+AGG - chr7_4:44719060-44719079 None:intergenic 45.0%
CTCTGCAGCTAACAAGAACA+AGG + chr7_4:44719061-44719080 Msa1156440:CDS 45.0%
AGCTAACAAGAACAAGGCTG+TGG + chr7_4:44719067-44719086 Msa1156440:CDS 45.0%
ACAAGAACAAGGCTGTGGAT+TGG + chr7_4:44719072-44719091 Msa1156440:CDS 45.0%
CAAGAACAAGGCTGTGGATT+GGG + chr7_4:44719073-44719092 Msa1156440:CDS 45.0%
! TTACTTGCTGAACCCCAAGA+AGG + chr7_4:44719115-44719134 Msa1156440:CDS 45.0%
CAATGAGGTGTACCTTCTTG+GGG - chr7_4:44719130-44719149 None:intergenic 45.0%
GAAAAACCATCTTCGTCCCT+GGG - chr7_4:44719389-44719408 None:intergenic 45.0%
TCTTGAGGCTTCTTAAGACC+AGG - chr7_4:44719411-44719430 None:intergenic 45.0%
!!! GGTGCAATAGCCACTTTTCT+TGG + chr7_4:44719551-44719570 Msa1156440:three_prime_UTR 45.0%
! CTTCTTGAGGGTGCGTATAT+TGG + chr7_4:44719632-44719651 Msa1156440:three_prime_UTR 45.0%
! ATGTGGATTGAGAGGAGGAT+AGG + chr7_4:44719858-44719877 Msa1156440:three_prime_UTR 45.0%
!!! TTTTTAAAAAATATATTGTA+AGG + chr7_4:44718324-44718343 Msa1156440:intron 5.0%
GTGATTTCGGTGGCCCAAAA+TGG + chr7_4:44717334-44717353 Msa1156440:five_prime_UTR 50.0%
ATTTCGGTGGCCCAAAATGG+CGG + chr7_4:44717337-44717356 Msa1156440:five_prime_UTR 50.0%
! GGAAAGCTACACCGCCATTT+TGG - chr7_4:44717351-44717370 None:intergenic 50.0%
TTTGAAGATCTTCTCGCCGG+CGG - chr7_4:44717524-44717543 None:intergenic 50.0%
GCGTTGTCAATCTTGGATCG+TGG + chr7_4:44718110-44718129 Msa1156440:intron 50.0%
CTGTTACAGTGCCGCAATAG+AGG + chr7_4:44718160-44718179 Msa1156440:intron 50.0%
GTTCAAACCAGGCAAGGAGA+GGG + chr7_4:44718404-44718423 Msa1156440:intron 50.0%
GCTGCAGAGTAGGAATATCC+AGG - chr7_4:44719050-44719069 None:intergenic 50.0%
GGAAAAACCATCTTCGTCCC+TGG - chr7_4:44719390-44719409 None:intergenic 50.0%
! GGGACGAAGATGGTTTTTCC+TGG + chr7_4:44719390-44719409 Msa1156440:CDS 50.0%
TCCTTCGATGAAGCTCCTCC+CGG + chr7_4:44717490-44717509 Msa1156440:CDS 55.0%
GGTTCAAACCAGGCAAGGAG+AGG + chr7_4:44718403-44718422 Msa1156440:intron 55.0%
! AGGCAATCTGGGACAACTCC+TGG + chr7_4:44719029-44719048 Msa1156440:CDS 55.0%
!! GAACAAGGCTGTGGATTGGG+AGG + chr7_4:44719076-44719095 Msa1156440:CDS 55.0%
GTACATCCCAGGGACGAAGA+TGG + chr7_4:44719380-44719399 Msa1156440:intron 55.0%
TCCGGGAGGAGCTTCATCGA+AGG - chr7_4:44717494-44717513 None:intergenic 60.0%
CAGTGCCACACCGTCGACAA+AGG + chr7_4:44717556-44717575 Msa1156440:CDS 60.0%
!! AAAGGCGCCGGCCACAAACA+AGG + chr7_4:44717574-44717593 Msa1156440:CDS 60.0%
ACCAGGCAAGGAGAGGGTTC+TGG + chr7_4:44718410-44718429 Msa1156440:intron 60.0%
ACCAGAACCCTCTCCTTGCC+TGG - chr7_4:44718414-44718433 None:intergenic 60.0%
AAGATCTTCTCGCCGGCGGC+GGG - chr7_4:44717520-44717539 None:intergenic 65.0%
GAAGATCTTCTCGCCGGCGG+CGG - chr7_4:44717521-44717540 None:intergenic 65.0%
! GGTGTGGCACTGAGCGCACT+TGG - chr7_4:44717548-44717567 None:intergenic 65.0%
! CACACCGTCGACAAAGGCGC+CGG + chr7_4:44717562-44717581 Msa1156440:CDS 65.0%
AGATCTTCTCGCCGGCGGCG+GGG - chr7_4:44717519-44717538 None:intergenic 70.0%
CGGCGCCTTTGTCGACGGTG+TGG - chr7_4:44717564-44717583 None:intergenic 70.0%
GTGGCCGGCGCCTTTGTCGA+CGG - chr7_4:44717569-44717588 None:intergenic 70.0%
! CCTCCCGGAAACCCCGCCGC+CGG + chr7_4:44717505-44717524 Msa1156440:CDS 80.0%
! TCGCCGGCGGCGGGGTTTCC+GGG - chr7_4:44717511-44717530 None:intergenic 80.0%
! CTCGCCGGCGGCGGGGTTTC+CGG - chr7_4:44717512-44717531 None:intergenic 80.0%
! CCGGCGGCGGGGTTTCCGGG+AGG - chr7_4:44717508-44717527 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 44717087 44719925 44717087 ID=Msa1156440;Name=Msa1156440
chr7_4 mRNA 44717087 44719925 44717087 ID=Msa1156440-mRNA-1;Parent=Msa1156440;Name=Msa1156440-mRNA-1;_AED=0.25;_eAED=0.25;_QI=397|1|1|1|1|1|3|449|113
chr7_4 exon 44717087 44717595 44717087 ID=Msa1156440-mRNA-1:exon:11563;Parent=Msa1156440-mRNA-1
chr7_4 exon 44719003 44719136 44719003 ID=Msa1156440-mRNA-1:exon:11564;Parent=Msa1156440-mRNA-1
chr7_4 exon 44719381 44719925 44719381 ID=Msa1156440-mRNA-1:exon:11565;Parent=Msa1156440-mRNA-1
chr7_4 five_prime_UTR 44717087 44717483 44717087 ID=Msa1156440-mRNA-1:five_prime_utr;Parent=Msa1156440-mRNA-1
chr7_4 CDS 44717484 44717595 44717484 ID=Msa1156440-mRNA-1:cds;Parent=Msa1156440-mRNA-1
chr7_4 CDS 44719003 44719136 44719003 ID=Msa1156440-mRNA-1:cds;Parent=Msa1156440-mRNA-1
chr7_4 CDS 44719381 44719476 44719381 ID=Msa1156440-mRNA-1:cds;Parent=Msa1156440-mRNA-1
chr7_4 three_prime_UTR 44719477 44719925 44719477 ID=Msa1156440-mRNA-1:three_prime_utr;Parent=Msa1156440-mRNA-1
Gene Sequence

>Msa1156440

ATGGCGTCCTTCGATGAAGCTCCTCCCGGAAACCCCGCCGCCGGCGAGAAGATCTTCAAAACCAAGTGCGCTCAGTGCCACACCGTCGACAAAGGCGCCGGCCACAAACAAGGACCCAATCTGAATGGTTTGTTTGGAAGGCAATCTGGGACAACTCCTGGATATTCCTACTCTGCAGCTAACAAGAACAAGGCTGTGGATTGGGAGGAAAAGACCTTGTACGATTACTTGCTGAACCCCAAGAAGTACATCCCAGGGACGAAGATGGTTTTTCCTGGTCTTAAGAAGCCTCAAGAACGTGCTGATCTTATTGCATATCTGAAAGATGCCACTTCAAAATAA
Protein sequence

>Msa1156440

MASFDEAPPGNPAAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSAANKNKAVDWEEKTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLIAYLKDATSK*