AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1068310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1068310 MtrunA17_Chr7g0249831 99.115 113 1 0 1 113 1 113 7.09e-82 234
Msa1068310 MtrunA17_Chr5g0396151 88.496 113 13 0 1 113 1 113 1.71e-73 213
Msa1068310 MtrunA17_Chr7g0249821 70.370 108 31 1 1 108 1 107 1.24e-51 158
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1068310 AT4G10040.1 91.071 112 10 0 1 112 1 112 4.58e-75 218
Msa1068310 AT1G22840.1 89.286 112 12 0 1 112 1 112 2.16e-73 213
Msa1068310 AT1G22840.2 88.095 84 10 0 1 84 1 84 4.72e-52 159

Find 76 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAATTTATTTATCTTAA+AGG 0.085752 7_2:-63283863 None:intergenic
TTTCATTCATGCTAATATTT+TGG 0.129707 7_2:-63283806 None:intergenic
CTCGCCGGCGGCGGGGTTTC+CGG 0.164134 7_2:-63284204 None:intergenic
GGAAAGCTACACCGCCATTT+TGG 0.196265 7_2:-63284043 None:intergenic
GTAGCTTTCCTATAACTTTC+GGG 0.235809 7_2:+63284056 Msa1068310:five_prime_UTR
ATGTATGACCCGAAAGTTAT+AGG 0.236852 7_2:-63284064 None:intergenic
TCGCCGGCGGCGGGGTTTCC+GGG 0.240370 7_2:-63284203 None:intergenic
TGTAGCTTTCCTATAACTTT+CGG 0.256249 7_2:+63284055 Msa1068310:five_prime_UTR
ATCAATGTGGTGTACCTTCT+TGG 0.286109 7_2:-63286636 None:intergenic
TCAATGTGGTGTACCTTCTT+GGG 0.302950 7_2:-63286635 None:intergenic
CCCAATCTGAATGGTTTGTT+TGG 0.305862 7_2:+63286512 Msa1068310:CDS
GGTTTGTTTGGAAGGCAATC+TGG 0.326375 7_2:+63286524 Msa1068310:CDS
GTACGAAAATTTAAAATTCC+AGG 0.329136 7_2:-63287023 None:intergenic
AAGATCTTCTCGCCGGCGGC+GGG 0.329325 7_2:-63284212 None:intergenic
TTCCTCTTAATTTCACAAAC+TGG 0.335331 7_2:+63287332 Msa1068310:three_prime_UTR
CTTCTTGAGGGTGCGTATAT+TGG 0.347584 7_2:+63287139 Msa1068310:three_prime_UTR
CTCCAGTTTGTGAAATTAAG+AGG 0.347757 7_2:-63287334 None:intergenic
TGCATTGCATGGCTTCTTGA+GGG 0.365075 7_2:+63287127 Msa1068310:three_prime_UTR
GTGATTTCGGTGGCCCAAAA+TGG 0.367212 7_2:+63284029 Msa1068310:five_prime_UTR
GACGCCATTTGAGAAAGTTC+TGG 0.371487 7_2:-63284164 None:intergenic
CAAGAACAAGGCTGTGGATT+GGG 0.392913 7_2:+63286580 Msa1068310:CDS
GCTAACAAAATCGAATGAAA+TGG 0.416689 7_2:+63283935 Msa1068310:five_prime_UTR
GTTTCCAGAACTTTCTCAAA+TGG 0.417788 7_2:+63284160 Msa1068310:exon
GAAGATCTTCTCGCCGGCGG+CGG 0.418146 7_2:-63284213 None:intergenic
TCCAAACAAACCATTCAGAT+TGG 0.424075 7_2:-63286513 None:intergenic
ATCTGAATGGTTTGTTTGGA+AGG 0.441498 7_2:+63286516 Msa1068310:CDS
AGGCAATCTGGGACAACTCC+TGG 0.447271 7_2:+63286536 Msa1068310:CDS
AAACTGGAGTTCTTTGTATG+TGG 0.451950 7_2:+63287348 Msa1068310:three_prime_UTR
TTCTTTGTATGTGGATTGAG+AGG 0.456920 7_2:+63287357 Msa1068310:three_prime_UTR
ATCTTAATGCAGTACATCCC+AGG 0.460110 7_2:+63286876 Msa1068310:intron
TTGCATTGCATGGCTTCTTG+AGG 0.465523 7_2:+63287126 Msa1068310:three_prime_UTR
GTTTGTTTGGAAGGCAATCT+GGG 0.482470 7_2:+63286525 Msa1068310:CDS
CCAAACAAACCATTCAGATT+GGG 0.484079 7_2:-63286512 None:intergenic
AATTTATTTATCTTAAAGGA+TGG 0.485319 7_2:-63283859 None:intergenic
AGAATAGTAAACCTTGTTTG+TGG 0.491881 7_2:-63284280 None:intergenic
ACCTTAGGACCCAATCTGAA+TGG 0.493293 7_2:+63286503 Msa1068310:intron
GCTTTATTTGCCAAGAAAAG+TGG 0.493817 7_2:-63287068 None:intergenic
TCCTTCGATGAAGCTCCTCC+CGG 0.495242 7_2:+63284185 Msa1068310:CDS
ACAAGAACAAGGCTGTGGAT+TGG 0.503827 7_2:+63286579 Msa1068310:CDS
AAGAAGCCATGCAATGCAAA+AGG 0.507620 7_2:-63287122 None:intergenic
TAGTAAACCTTGTTTGTGGC+CGG 0.509984 7_2:-63284276 None:intergenic
GTGGCCGGCGCCTTTGTCGA+CGG 0.510944 7_2:-63284261 None:intergenic
GTGTTGCGAAAGAATTGTAT+TGG 0.519266 7_2:-63284130 None:intergenic
GGTGTGGCACTGAGCGCACT+TGG 0.520968 7_2:-63284240 None:intergenic
CCTTTAAGATAAATAAATTG+AGG 0.523855 7_2:+63283863 Msa1068310:five_prime_UTR
CAATGTGGTGTACCTTCTTG+GGG 0.542057 7_2:-63286634 None:intergenic
GCTGCAGAGTAGGAATATCC+AGG 0.542193 7_2:-63286554 None:intergenic
ATGTGGATTGAGAGGAGGAT+AGG 0.545231 7_2:+63287365 Msa1068310:three_prime_UTR
TCTTGAGGCTTCTTAAGACC+AGG 0.550991 7_2:-63286915 None:intergenic
CCTCCCGGAAACCCCGCCGC+CGG 0.551439 7_2:+63284200 Msa1068310:CDS
TGAGATTCAGTTTATTGACC+TGG 0.552995 7_2:+63287407 Msa1068310:three_prime_UTR
AGATCTTCTCGCCGGCGGCG+GGG 0.553653 7_2:-63284211 None:intergenic
GGATAGGTTGAATTTCAAGT+TGG 0.556911 7_2:+63287381 Msa1068310:three_prime_UTR
GTTCTTGTTAGCTGCAGAGT+AGG 0.557927 7_2:-63286564 None:intergenic
AGTAAACAAAATCACAAGAC+TGG 0.564575 7_2:-63287270 None:intergenic
AGACTAATATTTGAACACAT+TGG 0.581329 7_2:+63287164 Msa1068310:three_prime_UTR
CAGTGCCACACCGTCGACAA+AGG 0.583975 7_2:+63284251 Msa1068310:CDS
TTTGTATGTGGATTGAGAGG+AGG 0.585787 7_2:+63287360 Msa1068310:three_prime_UTR
ATAAGATCAGCACGTTCTTG+AGG 0.588809 7_2:-63286930 None:intergenic
GTACATCCCAGGGACGAAGA+TGG 0.591227 7_2:+63286887 Msa1068310:intron
TTTGAAGATCTTCTCGCCGG+CGG 0.594334 7_2:-63284216 None:intergenic
TCCGGGAGGAGCTTCATCGA+AGG 0.600967 7_2:-63284186 None:intergenic
AAAGGCGCCGGCCACAAACA+AGG 0.601379 7_2:+63284269 Msa1068310:CDS
ACTGTTTGAGCACAAGCACC+AGG 0.602572 7_2:-63287425 None:intergenic
CTCTGCAGCTAACAAGAACA+AGG 0.605687 7_2:+63286568 Msa1068310:CDS
AGCTAACAAGAACAAGGCTG+TGG 0.608122 7_2:+63286574 Msa1068310:CDS
TCTTAATGCAGTACATCCCA+GGG 0.608747 7_2:+63286877 Msa1068310:intron
CACACCGTCGACAAAGGCGC+CGG 0.616193 7_2:+63284257 Msa1068310:CDS
GTTCAGCAAGTAATCGTACA+AGG 0.624998 7_2:-63286612 None:intergenic
TTGAGAAAGTTCTGGAAACG+AGG 0.625386 7_2:-63284156 None:intergenic
GAACAAGGCTGTGGATTGGG+AGG 0.635538 7_2:+63286583 Msa1068310:CDS
TTACTTGCTGAACCCCAAGA+AGG 0.638569 7_2:+63286622 Msa1068310:CDS
CGGCGCCTTTGTCGACGGTG+TGG 0.644445 7_2:-63284256 None:intergenic
CCGGCGGCGGGGTTTCCGGG+AGG 0.653806 7_2:-63284200 None:intergenic
ATTTCGGTGGCCCAAAATGG+CGG 0.654704 7_2:+63284032 Msa1068310:five_prime_UTR
TCGTTGACACGAACATTACG+CGG 0.669349 7_2:-63283907 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATATAATATTTATTGCA+AGG + chr7_2:63285256-63285275 Msa1068310:intron 10.0%
!! AATTTATTTATCTTAAAGGA+TGG - chr7_2:63283862-63283881 None:intergenic 15.0%
!! AAAAAATGAAAAAGTGATTT+CGG + chr7_2:63284016-63284035 Msa1068310:five_prime_UTR 15.0%
!! TTGTTATATTTACATAGTTT+AGG + chr7_2:63284973-63284992 Msa1068310:intron 15.0%
!! TGTTATATTTACATAGTTTA+GGG + chr7_2:63284974-63284993 Msa1068310:intron 15.0%
!! ACAATTATTGAAATAACATT+GGG - chr7_2:63285338-63285357 None:intergenic 15.0%
!! TACAATTATTGAAATAACAT+TGG - chr7_2:63285339-63285358 None:intergenic 15.0%
!!! AAAAAAAAGTAGATTTTTAC+CGG + chr7_2:63285803-63285822 Msa1068310:intron 15.0%
!!! ACTTCAATTTATTTTTAGTT+TGG - chr7_2:63285871-63285890 None:intergenic 15.0%
!! TTCATATGATAATTAAATAG+AGG - chr7_2:63286036-63286055 None:intergenic 15.0%
!! TTAATTATCATATGAATAAG+AGG + chr7_2:63286040-63286059 Msa1068310:intron 15.0%
!! TTAATTCATTATCAGTTAAT+GGG - chr7_2:63286088-63286107 None:intergenic 15.0%
!! TTTAATTCATTATCAGTTAA+TGG - chr7_2:63286089-63286108 None:intergenic 15.0%
!!! AACTGATAATGAATTAAATT+AGG + chr7_2:63286091-63286110 Msa1068310:intron 15.0%
!! CATATGTGAAAAATATTAAA+CGG - chr7_2:63286321-63286340 None:intergenic 15.0%
!! ATAAAAATGTCTATATCAAA+TGG + chr7_2:63286688-63286707 Msa1068310:intron 15.0%
!!! TAAATTCTATTATTTTGAAG+TGG - chr7_2:63286973-63286992 None:intergenic 15.0%
!!! TTTCATTCATGCTAATATTT+TGG - chr7_2:63283809-63283828 None:intergenic 20.0%
!! CCTCAATTTATTTATCTTAA+AGG - chr7_2:63283866-63283885 None:intergenic 20.0%
!!! CCTTTAAGATAAATAAATTG+AGG + chr7_2:63283863-63283882 Msa1068310:five_prime_UTR 20.0%
!! ATTTGATCTCTAAATATCTT+GGG + chr7_2:63284732-63284751 Msa1068310:intron 20.0%
!! TAAATATAACAAGTAATCTG+AGG - chr7_2:63284965-63284984 None:intergenic 20.0%
!!! AAATGAATAAACCATTGATT+TGG - chr7_2:63285001-63285020 None:intergenic 20.0%
!!! ATGAAAATGTTTTGTAAACT+TGG + chr7_2:63285058-63285077 Msa1068310:intron 20.0%
!! CAATTATTGAAATAACATTG+GGG - chr7_2:63285337-63285356 None:intergenic 20.0%
!! TTAAACGGCATAAATAATAA+GGG - chr7_2:63286306-63286325 None:intergenic 20.0%
!! ATTAAACGGCATAAATAATA+AGG - chr7_2:63286307-63286326 None:intergenic 20.0%
!! TTACAAAATGACATTATTCA+AGG - chr7_2:63286445-63286464 None:intergenic 20.0%
!!! GAATGAAATGGATTTTATTG+TGG + chr7_2:63283947-63283966 Msa1068310:five_prime_UTR 25.0%
! AAAAAACGAAAAAACATCAC+AGG - chr7_2:63284347-63284366 None:intergenic 25.0%
! TAAGAATCTATTGTATTGTG+TGG + chr7_2:63284463-63284482 Msa1068310:intron 25.0%
!! AATACTTATGTAAGTTGGAT+CGG - chr7_2:63284584-63284603 None:intergenic 25.0%
! TCCAACTTACATAAGTATTA+AGG + chr7_2:63284585-63284604 Msa1068310:intron 25.0%
! ACCTTAATACTTATGTAAGT+TGG - chr7_2:63284589-63284608 None:intergenic 25.0%
! AATCCGTAAACTATAGAAAT+CGG + chr7_2:63284688-63284707 Msa1068310:intron 25.0%
! CATTTGATCTCTAAATATCT+TGG + chr7_2:63284731-63284750 Msa1068310:intron 25.0%
! TTGTGAAAAAAACCAAAAGA+TGG - chr7_2:63285170-63285189 None:intergenic 25.0%
!!! ATCTTTTGGTTTTTTTCACA+AGG + chr7_2:63285169-63285188 Msa1068310:intron 25.0%
! TGAATGAATTGTGATTAGAA+AGG + chr7_2:63285390-63285409 Msa1068310:intron 25.0%
! AAACTCAAGGGATTATTATT+TGG + chr7_2:63285484-63285503 Msa1068310:intron 25.0%
! AACTCAAGGGATTATTATTT+GGG + chr7_2:63285485-63285504 Msa1068310:intron 25.0%
! GAAGAATAGATCAACAATTT+GGG - chr7_2:63285685-63285704 None:intergenic 25.0%
! TGAAGAATAGATCAACAATT+TGG - chr7_2:63285686-63285705 None:intergenic 25.0%
!!! TTTATTTTTAGTTTGGTCAC+CGG - chr7_2:63285864-63285883 None:intergenic 25.0%
!!! CTCAGATTTTTTTGTTTGTT+GGG - chr7_2:63285901-63285920 None:intergenic 25.0%
!!! TCTCAGATTTTTTTGTTTGT+TGG - chr7_2:63285902-63285921 None:intergenic 25.0%
! ATTATCATATGAATAAGAGG+TGG + chr7_2:63286043-63286062 Msa1068310:intron 25.0%
!!! TTGAGTTGAAACAATTGAAT+GGG + chr7_2:63286118-63286137 Msa1068310:intron 25.0%
!!! ACCCATGAACTTTTTTTAAA+GGG + chr7_2:63286265-63286284 Msa1068310:intron 25.0%
!! ACCCTTTAAAAAAAGTTCAT+GGG - chr7_2:63286269-63286288 None:intergenic 25.0%
!!! TTTTTTAAAGGGTGCATATT+AGG + chr7_2:63286276-63286295 Msa1068310:intron 25.0%
!!! TTTTTAAAGGGTGCATATTA+GGG + chr7_2:63286277-63286296 Msa1068310:intron 25.0%
!! CATTTAGTGTTTTCTACAAT+TGG + chr7_2:63286755-63286774 Msa1068310:intron 25.0%
! GTTATGCTGTTAATTTATTG+TGG + chr7_2:63286784-63286803 Msa1068310:intron 25.0%
!!! ATTGTGGTTATGTTGATAAT+AGG + chr7_2:63286800-63286819 Msa1068310:intron 25.0%
!! GTACGAAAATTTAAAATTCC+AGG - chr7_2:63287026-63287045 None:intergenic 25.0%
!! TTTTCGTACATTTGATTATG+TGG + chr7_2:63287037-63287056 Msa1068310:three_prime_UTR 25.0%
! AGACTAATATTTGAACACAT+TGG + chr7_2:63287164-63287183 Msa1068310:three_prime_UTR 25.0%
GCTAACAAAATCGAATGAAA+TGG + chr7_2:63283935-63283954 Msa1068310:five_prime_UTR 30.0%
AAATGAAAAAGTGATTTCGG+TGG + chr7_2:63284019-63284038 Msa1068310:five_prime_UTR 30.0%
TGTAGCTTTCCTATAACTTT+CGG + chr7_2:63284055-63284074 Msa1068310:five_prime_UTR 30.0%
! AGAGAATATGCTATGCTTTT+TGG - chr7_2:63284095-63284114 None:intergenic 30.0%
! AGAATAGTAAACCTTGTTTG+TGG - chr7_2:63284283-63284302 None:intergenic 30.0%
GAAAAAACTTAACAGCGATT+AGG - chr7_2:63284401-63284420 None:intergenic 30.0%
TGATTGTTCAAGATCTTGTT+AGG + chr7_2:63284437-63284456 Msa1068310:intron 30.0%
GATTGTTCAAGATCTTGTTA+GGG + chr7_2:63284438-63284457 Msa1068310:intron 30.0%
TGTTGAAGCTTGAAATGTTT+AGG + chr7_2:63284495-63284514 Msa1068310:intron 30.0%
ACTCCGATTTCTATAGTTTA+CGG - chr7_2:63284694-63284713 None:intergenic 30.0%
ACTCAGCTAAAAAAACAAGA+AGG - chr7_2:63285450-63285469 None:intergenic 30.0%
!!! TCTTGTTTTTTTAGCTGAGT+CGG + chr7_2:63285450-63285469 Msa1068310:intron 30.0%
GTTGAATGAACAAAACTCAA+GGG + chr7_2:63285472-63285491 Msa1068310:intron 30.0%
! CTTTAAAAGGGACCATATTA+GGG - chr7_2:63285514-63285533 None:intergenic 30.0%
! TCTTTAAAAGGGACCATATT+AGG - chr7_2:63285515-63285534 None:intergenic 30.0%
!! TAATATGGTCCCTTTTAAAG+AGG + chr7_2:63285514-63285533 Msa1068310:intron 30.0%
AAGATACAAGCAGAAAATAG+GGG - chr7_2:63285622-63285641 None:intergenic 30.0%
GAAGATACAAGCAGAAAATA+GGG - chr7_2:63285623-63285642 None:intergenic 30.0%
AGAAGATACAAGCAGAAAAT+AGG - chr7_2:63285624-63285643 None:intergenic 30.0%
!! TTGGGAATTTTGATGAACAA+CGG - chr7_2:63285667-63285686 None:intergenic 30.0%
! AAAAAGTAGATTTTTACCGG+TGG + chr7_2:63285806-63285825 Msa1068310:intron 30.0%
!!! TTTTTTGTTTGTTGGGATGT+TGG - chr7_2:63285894-63285913 None:intergenic 30.0%
CAAAAAAATCTGAGAACACT+CGG + chr7_2:63285907-63285926 Msa1068310:intron 30.0%
!! GTTGAGTTGAAACAATTGAA+TGG + chr7_2:63286117-63286136 Msa1068310:intron 30.0%
GGTTAAAAAAGGATAGAACA+AGG - chr7_2:63286161-63286180 None:intergenic 30.0%
TGTTCAGTCTGTACTTTAAA+GGG + chr7_2:63286183-63286202 Msa1068310:intron 30.0%
CAAAGAATAATTCAGTATCC+TGG + chr7_2:63286208-63286227 Msa1068310:intron 30.0%
! TCATATCCGGTATTTTTCTA+AGG + chr7_2:63286239-63286258 Msa1068310:intron 30.0%
!!! CACCCATGAACTTTTTTTAA+AGG + chr7_2:63286264-63286283 Msa1068310:intron 30.0%
! CACCCTTTAAAAAAAGTTCA+TGG - chr7_2:63286270-63286289 None:intergenic 30.0%
!! TTTTAAAGGGTGCATATTAG+GGG + chr7_2:63286278-63286297 Msa1068310:intron 30.0%
AATGACATTATTCAAGGAGA+GGG - chr7_2:63286439-63286458 None:intergenic 30.0%
AAATGACATTATTCAAGGAG+AGG - chr7_2:63286440-63286459 None:intergenic 30.0%
! TAGTGTTTTCTACAATTGGT+AGG + chr7_2:63286759-63286778 Msa1068310:intron 30.0%
ACGCATATCAGTAAAATACT+TGG - chr7_2:63286848-63286867 None:intergenic 30.0%
AGTAAACAAAATCACAAGAC+TGG - chr7_2:63287273-63287292 None:intergenic 30.0%
TTCCTCTTAATTTCACAAAC+TGG + chr7_2:63287332-63287351 Msa1068310:three_prime_UTR 30.0%
GTAGCTTTCCTATAACTTTC+GGG + chr7_2:63284056-63284075 Msa1068310:five_prime_UTR 35.0%
ATGTATGACCCGAAAGTTAT+AGG - chr7_2:63284067-63284086 None:intergenic 35.0%
GTGTTGCGAAAGAATTGTAT+TGG - chr7_2:63284133-63284152 None:intergenic 35.0%
GTTTCCAGAACTTTCTCAAA+TGG + chr7_2:63284160-63284179 Msa1068310:exon 35.0%
!! CGTAGATCTGAGAGTATTAA+GGG - chr7_2:63284321-63284340 None:intergenic 35.0%
TTGGTTCCCTAATCTCTTAA+TGG + chr7_2:63284768-63284787 Msa1068310:intron 35.0%
TCTCAGCCATTAAGAGATTA+GGG - chr7_2:63284777-63284796 None:intergenic 35.0%
ATCTCAGCCATTAAGAGATT+AGG - chr7_2:63284778-63284797 None:intergenic 35.0%
TTAAACAGCGTTGTCAATCT+TGG + chr7_2:63284797-63284816 Msa1068310:intron 35.0%
TTGTCAAAAAGCCTCTATTG+CGG - chr7_2:63284868-63284887 None:intergenic 35.0%
CAACACTTTACACTGAATAG+TGG + chr7_2:63284885-63284904 Msa1068310:intron 35.0%
TAGTTTAGGGACCAAATCAA+TGG + chr7_2:63284987-63285006 Msa1068310:intron 35.0%
CTTGGTATGTTCAGAATGTT+TGG + chr7_2:63285076-63285095 Msa1068310:intron 35.0%
!!! GTTTTGTTTGAGAGATGAGA+GGG + chr7_2:63285137-63285156 Msa1068310:intron 35.0%
GAAATAACATTGGGGTGATA+TGG - chr7_2:63285329-63285348 None:intergenic 35.0%
GGTTGAATGAACAAAACTCA+AGG + chr7_2:63285471-63285490 Msa1068310:intron 35.0%
TTATTTGGGTGTCCCTAATA+TGG + chr7_2:63285499-63285518 Msa1068310:intron 35.0%
TATAGCAGGTTCACTTGAAT+GGG - chr7_2:63285552-63285571 None:intergenic 35.0%
ATGTTATAACCTGCTATAGC+AGG - chr7_2:63285566-63285585 None:intergenic 35.0%
AGCAGAAAATAGGGGATAAT+TGG - chr7_2:63285614-63285633 None:intergenic 35.0%
!! TGGGAATTTTGATGAACAAC+GGG - chr7_2:63285666-63285685 None:intergenic 35.0%
CAAATTCATGAAGTTTCCAC+CGG - chr7_2:63285825-63285844 None:intergenic 35.0%
TATGTCAGAGTTGCAACTTA+CGG - chr7_2:63285994-63286013 None:intergenic 35.0%
GACTGAACAGTGGTTAAAAA+AGG - chr7_2:63286172-63286191 None:intergenic 35.0%
TTAAAGTACAGACTGAACAG+TGG - chr7_2:63286182-63286201 None:intergenic 35.0%
CTGTTCAGTCTGTACTTTAA+AGG + chr7_2:63286182-63286201 Msa1068310:intron 35.0%
AGAAAAATACCGGATATGAC+CGG - chr7_2:63286238-63286257 None:intergenic 35.0%
GGTGAACCTTAGAAAAATAC+CGG - chr7_2:63286248-63286267 None:intergenic 35.0%
CCAAACAAACCATTCAGATT+GGG - chr7_2:63286515-63286534 None:intergenic 35.0%
TCCAAACAAACCATTCAGAT+TGG - chr7_2:63286516-63286535 None:intergenic 35.0%
!! ATCTGAATGGTTTGTTTGGA+AGG + chr7_2:63286516-63286535 Msa1068310:CDS 35.0%
! TGTTTTCTACAATTGGTAGG+AGG + chr7_2:63286762-63286781 Msa1068310:intron 35.0%
TTGGTTGAAACAGACTTTCA+AGG - chr7_2:63286829-63286848 None:intergenic 35.0%
!! GCTTTATTTGCCAAGAAAAG+TGG - chr7_2:63287071-63287090 None:intergenic 35.0%
! TTTGTTCCTTTTGCATTGCA+TGG + chr7_2:63287116-63287135 Msa1068310:three_prime_UTR 35.0%
CTCCAGTTTGTGAAATTAAG+AGG - chr7_2:63287337-63287356 None:intergenic 35.0%
AAACTGGAGTTCTTTGTATG+TGG + chr7_2:63287348-63287367 Msa1068310:three_prime_UTR 35.0%
! TTCTTTGTATGTGGATTGAG+AGG + chr7_2:63287357-63287376 Msa1068310:three_prime_UTR 35.0%
! GGATAGGTTGAATTTCAAGT+TGG + chr7_2:63287381-63287400 Msa1068310:three_prime_UTR 35.0%
TGAGATTCAGTTTATTGACC+TGG + chr7_2:63287407-63287426 Msa1068310:three_prime_UTR 35.0%
!! TTGAGAAAGTTCTGGAAACG+AGG - chr7_2:63284159-63284178 None:intergenic 40.0%
! TAGTAAACCTTGTTTGTGGC+CGG - chr7_2:63284279-63284298 None:intergenic 40.0%
!! GCGTAGATCTGAGAGTATTA+AGG - chr7_2:63284322-63284341 None:intergenic 40.0%
GGATCGGACATAACTGAAAT+TGG - chr7_2:63284568-63284587 None:intergenic 40.0%
! CTTGGGCTTAAATGCACTTT+TGG + chr7_2:63284749-63284768 Msa1068310:intron 40.0%
! CAGAATGTTTGGTTCAAACC+AGG + chr7_2:63285087-63285106 Msa1068310:intron 40.0%
!!! CGTTTTGTTTGAGAGATGAG+AGG + chr7_2:63285136-63285155 Msa1068310:intron 40.0%
!!! GGTTTTTTTCACAAGGAGAG+AGG + chr7_2:63285176-63285195 Msa1068310:intron 40.0%
!! TCCCTTTTAAAGAGGTTCAC+CGG + chr7_2:63285522-63285541 Msa1068310:intron 40.0%
! ACCGGTGAACCTCTTTAAAA+GGG - chr7_2:63285526-63285545 None:intergenic 40.0%
CTATAGCAGGTTCACTTGAA+TGG - chr7_2:63285553-63285572 None:intergenic 40.0%
!! GGGAATTTTGATGAACAACG+GGG - chr7_2:63285665-63285684 None:intergenic 40.0%
TCAACAACACACAATTGCGT+TGG - chr7_2:63285766-63285785 None:intergenic 40.0%
! ACGCAATTGTGTGTTGTTGA+AGG + chr7_2:63285766-63285785 Msa1068310:intron 40.0%
!!! TTTTTAGTTTGGTCACCGGA+GGG - chr7_2:63285860-63285879 None:intergenic 40.0%
!!! ATTTTTAGTTTGGTCACCGG+AGG - chr7_2:63285861-63285880 None:intergenic 40.0%
AGTTGCAACTTACGGAAACT+TGG - chr7_2:63285986-63286005 None:intergenic 40.0%
! TTATCAGTTAATGGGCATCG+AGG - chr7_2:63286080-63286099 None:intergenic 40.0%
AAAAAGGATAGAACAAGGCG+TGG - chr7_2:63286156-63286175 None:intergenic 40.0%
AATTCAGTATCCTGGCTTAC+CGG + chr7_2:63286216-63286235 Msa1068310:intron 40.0%
! CTTTATTTGTTGCCGACCTT+AGG + chr7_2:63286488-63286507 Msa1068310:intron 40.0%
!! CCCAATCTGAATGGTTTGTT+TGG + chr7_2:63286512-63286531 Msa1068310:CDS 40.0%
GTTTGTTTGGAAGGCAATCT+GGG + chr7_2:63286525-63286544 Msa1068310:CDS 40.0%
GTTCAGCAAGTAATCGTACA+AGG - chr7_2:63286615-63286634 None:intergenic 40.0%
TCAATGTGGTGTACCTTCTT+GGG - chr7_2:63286638-63286657 None:intergenic 40.0%
ATCAATGTGGTGTACCTTCT+TGG - chr7_2:63286639-63286658 None:intergenic 40.0%
! AGAAGGTACACCACATTGAT+TGG + chr7_2:63286639-63286658 Msa1068310:intron 40.0%
CACAGAAATGCCAATCAATG+TGG - chr7_2:63286652-63286671 None:intergenic 40.0%
ATCTTAATGCAGTACATCCC+AGG + chr7_2:63286876-63286895 Msa1068310:intron 40.0%
TCTTAATGCAGTACATCCCA+GGG + chr7_2:63286877-63286896 Msa1068310:intron 40.0%
ATAAGATCAGCACGTTCTTG+AGG - chr7_2:63286933-63286952 None:intergenic 40.0%
! TGCAGTATGCTTTTCAAACC+TGG + chr7_2:63287005-63287024 Msa1068310:three_prime_UTR 40.0%
AAGAAGCCATGCAATGCAAA+AGG - chr7_2:63287125-63287144 None:intergenic 40.0%
! TTTGTATGTGGATTGAGAGG+AGG + chr7_2:63287360-63287379 Msa1068310:three_prime_UTR 40.0%
TCGTTGACACGAACATTACG+CGG - chr7_2:63283910-63283929 None:intergenic 45.0%
!! GAAAGCTACACCGCCATTTT+GGG - chr7_2:63284045-63284064 None:intergenic 45.0%
GACGCCATTTGAGAAAGTTC+TGG - chr7_2:63284167-63284186 None:intergenic 45.0%
!!! GGTTTTGAAGATCTTCTCGC+CGG - chr7_2:63284222-63284241 None:intergenic 45.0%
! TGTTTGGTTCAAACCAGGCA+AGG + chr7_2:63285092-63285111 Msa1068310:intron 45.0%
! GAGGGCAAACAACCATCTTT+TGG + chr7_2:63285155-63285174 Msa1068310:intron 45.0%
CACCGGTGAACCTCTTTAAA+AGG - chr7_2:63285527-63285546 None:intergenic 45.0%
! GTTCACTTGAATGGGTTCAC+CGG - chr7_2:63285544-63285563 None:intergenic 45.0%
TCAAGTGAACCTGCTATAGC+AGG + chr7_2:63285554-63285573 Msa1068310:intron 45.0%
TTGAAGACACACTTACCCTC+CGG + chr7_2:63285842-63285861 Msa1068310:intron 45.0%
CAACTTACGGAAACTTGGAC+AGG - chr7_2:63285981-63286000 None:intergenic 45.0%
! ATTATTCAAGGAGAGGGCAC+AGG - chr7_2:63286433-63286452 None:intergenic 45.0%
TTCAGATTGGGTCCTAAGGT+CGG - chr7_2:63286503-63286522 None:intergenic 45.0%
ACCTTAGGACCCAATCTGAA+TGG + chr7_2:63286503-63286522 Msa1068310:intron 45.0%
ACCATTCAGATTGGGTCCTA+AGG - chr7_2:63286507-63286526 None:intergenic 45.0%
! GGTTTGTTTGGAAGGCAATC+TGG + chr7_2:63286524-63286543 Msa1068310:CDS 45.0%
GTTCTTGTTAGCTGCAGAGT+AGG - chr7_2:63286567-63286586 None:intergenic 45.0%
CTCTGCAGCTAACAAGAACA+AGG + chr7_2:63286568-63286587 Msa1068310:CDS 45.0%
AGCTAACAAGAACAAGGCTG+TGG + chr7_2:63286574-63286593 Msa1068310:CDS 45.0%
ACAAGAACAAGGCTGTGGAT+TGG + chr7_2:63286579-63286598 Msa1068310:CDS 45.0%
CAAGAACAAGGCTGTGGATT+GGG + chr7_2:63286580-63286599 Msa1068310:CDS 45.0%
! TTACTTGCTGAACCCCAAGA+AGG + chr7_2:63286622-63286641 Msa1068310:CDS 45.0%
CAATGTGGTGTACCTTCTTG+GGG - chr7_2:63286637-63286656 None:intergenic 45.0%
GAAAAACCATCTTCGTCCCT+GGG - chr7_2:63286896-63286915 None:intergenic 45.0%
TCTTGAGGCTTCTTAAGACC+AGG - chr7_2:63286918-63286937 None:intergenic 45.0%
!!! GGTGCAATAGCCACTTTTCT+TGG + chr7_2:63287058-63287077 Msa1068310:three_prime_UTR 45.0%
TTGCATTGCATGGCTTCTTG+AGG + chr7_2:63287126-63287145 Msa1068310:three_prime_UTR 45.0%
TGCATTGCATGGCTTCTTGA+GGG + chr7_2:63287127-63287146 Msa1068310:three_prime_UTR 45.0%
! CTTCTTGAGGGTGCGTATAT+TGG + chr7_2:63287139-63287158 Msa1068310:three_prime_UTR 45.0%
! ATGTGGATTGAGAGGAGGAT+AGG + chr7_2:63287365-63287384 Msa1068310:three_prime_UTR 45.0%
!!! TTTTTAAAAAATATATTGTA+AGG + chr7_2:63285018-63285037 Msa1068310:intron 5.0%
GTGATTTCGGTGGCCCAAAA+TGG + chr7_2:63284029-63284048 Msa1068310:five_prime_UTR 50.0%
ATTTCGGTGGCCCAAAATGG+CGG + chr7_2:63284032-63284051 Msa1068310:five_prime_UTR 50.0%
! GGAAAGCTACACCGCCATTT+TGG - chr7_2:63284046-63284065 None:intergenic 50.0%
TTTGAAGATCTTCTCGCCGG+CGG - chr7_2:63284219-63284238 None:intergenic 50.0%
GCGTTGTCAATCTTGGATCG+TGG + chr7_2:63284804-63284823 Msa1068310:intron 50.0%
CTGTTACAGTGCCGCAATAG+AGG + chr7_2:63284854-63284873 Msa1068310:intron 50.0%
GTTCAAACCAGGCAAGGAGA+GGG + chr7_2:63285098-63285117 Msa1068310:intron 50.0%
!! AAACTTGGACAGGTGCCGAT+GGG - chr7_2:63285971-63285990 None:intergenic 50.0%
GCTGCAGAGTAGGAATATCC+AGG - chr7_2:63286557-63286576 None:intergenic 50.0%
GGAAAAACCATCTTCGTCCC+TGG - chr7_2:63286897-63286916 None:intergenic 50.0%
! GGGACGAAGATGGTTTTTCC+TGG + chr7_2:63286897-63286916 Msa1068310:CDS 50.0%
TCCTTCGATGAAGCTCCTCC+CGG + chr7_2:63284185-63284204 Msa1068310:CDS 55.0%
GGTTCAAACCAGGCAAGGAG+AGG + chr7_2:63285097-63285116 Msa1068310:intron 55.0%
!! GAAACTTGGACAGGTGCCGA+TGG - chr7_2:63285972-63285991 None:intergenic 55.0%
CCTGGCTTACCGGTCATATC+CGG + chr7_2:63286226-63286245 Msa1068310:intron 55.0%
! AGGCAATCTGGGACAACTCC+TGG + chr7_2:63286536-63286555 Msa1068310:CDS 55.0%
!! GAACAAGGCTGTGGATTGGG+AGG + chr7_2:63286583-63286602 Msa1068310:CDS 55.0%
GTACATCCCAGGGACGAAGA+TGG + chr7_2:63286887-63286906 Msa1068310:intron 55.0%
TCCGGGAGGAGCTTCATCGA+AGG - chr7_2:63284189-63284208 None:intergenic 60.0%
CAGTGCCACACCGTCGACAA+AGG + chr7_2:63284251-63284270 Msa1068310:CDS 60.0%
!! AAAGGCGCCGGCCACAAACA+AGG + chr7_2:63284269-63284288 Msa1068310:CDS 60.0%
ACCAGGCAAGGAGAGGGTTC+TGG + chr7_2:63285104-63285123 Msa1068310:intron 60.0%
ACCAGAACCCTCTCCTTGCC+TGG - chr7_2:63285108-63285127 None:intergenic 60.0%
!! CAACGGTGGTGGAACGGGAA+GGG - chr7_2:63285945-63285964 None:intergenic 60.0%
!! GGATCCAACGGTGGTGGAAC+GGG - chr7_2:63285950-63285969 None:intergenic 60.0%
!! GGGATCCAACGGTGGTGGAA+CGG - chr7_2:63285951-63285970 None:intergenic 60.0%
CCACCACCGTTGGATCCCAT+CGG + chr7_2:63285953-63285972 Msa1068310:intron 60.0%
!! CAGGTGCCGATGGGATCCAA+CGG - chr7_2:63285962-63285981 None:intergenic 60.0%
CCGGATATGACCGGTAAGCC+AGG - chr7_2:63286229-63286248 None:intergenic 60.0%
AAGATCTTCTCGCCGGCGGC+GGG - chr7_2:63284215-63284234 None:intergenic 65.0%
GAAGATCTTCTCGCCGGCGG+CGG - chr7_2:63284216-63284235 None:intergenic 65.0%
! GGTGTGGCACTGAGCGCACT+TGG - chr7_2:63284243-63284262 None:intergenic 65.0%
! CACACCGTCGACAAAGGCGC+CGG + chr7_2:63284257-63284276 Msa1068310:CDS 65.0%
!! CCAACGGTGGTGGAACGGGA+AGG - chr7_2:63285946-63285965 None:intergenic 65.0%
CCTTCCCGTTCCACCACCGT+TGG + chr7_2:63285943-63285962 Msa1068310:intron 65.0%
CCGATGGGATCCAACGGTGG+TGG - chr7_2:63285956-63285975 None:intergenic 65.0%
! GTGCCGATGGGATCCAACGG+TGG - chr7_2:63285959-63285978 None:intergenic 65.0%
AGATCTTCTCGCCGGCGGCG+GGG - chr7_2:63284214-63284233 None:intergenic 70.0%
CGGCGCCTTTGTCGACGGTG+TGG - chr7_2:63284259-63284278 None:intergenic 70.0%
GTGGCCGGCGCCTTTGTCGA+CGG - chr7_2:63284264-63284283 None:intergenic 70.0%
! CCTCCCGGAAACCCCGCCGC+CGG + chr7_2:63284200-63284219 Msa1068310:CDS 80.0%
! TCGCCGGCGGCGGGGTTTCC+GGG - chr7_2:63284206-63284225 None:intergenic 80.0%
! CTCGCCGGCGGCGGGGTTTC+CGG - chr7_2:63284207-63284226 None:intergenic 80.0%
! CCGGCGGCGGGGTTTCCGGG+AGG - chr7_2:63284203-63284222 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 63283790 63287432 63283790 ID=Msa1068310;Name=Msa1068310
chr7_2 mRNA 63283790 63287432 63283790 ID=Msa1068310-mRNA-1;Parent=Msa1068310;Name=Msa1068310-mRNA-1;_AED=0.01;_eAED=0.01;_QI=389|1|1|1|1|1|3|449|113
chr7_2 exon 63283790 63284290 63283790 ID=Msa1068310-mRNA-1:exon:13015;Parent=Msa1068310-mRNA-1
chr7_2 exon 63286510 63286643 63286510 ID=Msa1068310-mRNA-1:exon:13016;Parent=Msa1068310-mRNA-1
chr7_2 exon 63286888 63287432 63286888 ID=Msa1068310-mRNA-1:exon:13017;Parent=Msa1068310-mRNA-1
chr7_2 five_prime_UTR 63283790 63284178 63283790 ID=Msa1068310-mRNA-1:five_prime_utr;Parent=Msa1068310-mRNA-1
chr7_2 CDS 63284179 63284290 63284179 ID=Msa1068310-mRNA-1:cds;Parent=Msa1068310-mRNA-1
chr7_2 CDS 63286510 63286643 63286510 ID=Msa1068310-mRNA-1:cds;Parent=Msa1068310-mRNA-1
chr7_2 CDS 63286888 63286983 63286888 ID=Msa1068310-mRNA-1:cds;Parent=Msa1068310-mRNA-1
chr7_2 three_prime_UTR 63286984 63287432 63286984 ID=Msa1068310-mRNA-1:three_prime_utr;Parent=Msa1068310-mRNA-1
Gene Sequence

>Msa1068310

ATGGCGTCCTTCGATGAAGCTCCTCCCGGAAACCCCGCCGCCGGCGAGAAGATCTTCAAAACCAAGTGCGCTCAGTGCCACACCGTCGACAAAGGCGCCGGCCACAAACAAGGACCCAATCTGAATGGTTTGTTTGGAAGGCAATCTGGGACAACTCCTGGATATTCCTACTCTGCAGCTAACAAGAACAAGGCTGTGGATTGGGAGGAAAAGACCTTGTACGATTACTTGCTGAACCCCAAGAAGTACATCCCAGGGACGAAGATGGTTTTTCCTGGTCTTAAGAAGCCTCAAGAACGTGCTGATCTTATTGCATATCTGAAAGATGCCACTTCAAAATAA
Protein sequence

>Msa1068310

MASFDEAPPGNPAAGEKIFKTKCAQCHTVDKGAGHKQGPNLNGLFGRQSGTTPGYSYSAANKNKAVDWEEKTLYDYLLNPKKYIPGTKMVFPGLKKPQERADLIAYLKDATSK*