AlfalfaGEDB | Msa1159270

M. sativa cultivar zhongmu-4 / Msa1159270

KEGG: K09422
GO: GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000987 GO:0000988 GO:0000989 GO:0001012 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0001935 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0007275 GO:0008150 GO:0008283 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009741 GO:0009751 GO:0009753 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035670 GO:0040008 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046983 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048532 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048585 GO:0048586 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050673 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0051782 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0080050 GO:0080060 GO:0080090 GO:0080113 GO:0090567 GO:0097159 GO:0097305 GO:0098542 GO:0099402 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1904961 GO:1990837 GO:2000022 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000241 GO:2000242 GO:2001023 GO:2001038 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1159270	XP_003624741.1	96.369	358	11	1	1	358	1	356	0.0	702

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1159270	sp\|Q5NBM8\|CSA_ORYSJ	82.906	117	20	0	87	203	45	161	1.27e-72	232

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1159270	A2Q4D1	96.369	358	11	1	1	358	1	356	0.0	702

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TFs/TRs:

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Gene ID	Type	Classification
Msa1159270	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0241750	Msa1159270	0.807617	4.631691e-50	-8.615850e-47
Msa0795750	Msa1159270	0.818236	2.177724e-52	-8.615850e-47
Msa1032470	Msa1159270	0.859928	2.944464e-63	-8.615850e-47
Msa1072250	Msa1159270	0.822368	2.459190e-53	-8.615850e-47
Msa1159270	Msa1197720	0.808107	3.642642e-50	-8.615850e-47
Msa1159270	Msa1251410	0.814405	1.566720e-51	-8.615850e-47
Msa1159270	Msa1351990	0.851609	7.939851e-61	-8.615850e-47
Msa1159270	Msa1396200	-0.800774	1.231063e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1159270	MtrunA17_Chr7g0254261	96.369	358	11	1	1	358	1	356	0.0	702
Msa1159270	MtrunA17_Chr6g0460671	50.467	321	132	7	34	354	52	345	5.09e-81	251
Msa1159270	MtrunA17_Chr4g0042501	75.714	140	34	0	64	203	78	217	2.31e-75	239
Msa1159270	MtrunA17_Chr5g0417621	42.368	321	131	10	84	356	127	441	1.04e-71	229
Msa1159270	MtrunA17_Chr3g0125801	43.463	283	149	4	86	358	11	292	3.29e-63	203
Msa1159270	MtrunA17_Chr6g0480581	74.107	112	29	0	88	199	1	112	2.98e-61	196
Msa1159270	MtrunA17_Chr6g0460661	56.098	123	54	0	90	212	15	137	3.25e-46	155
Msa1159270	MtrunA17_Chr4g0008551	59.406	101	41	0	91	191	38	138	5.62e-40	144
Msa1159270	MtrunA17_Chr5g0401731	44.138	145	72	2	91	235	15	150	1.55e-37	137
Msa1159270	MtrunA17_Chr4g0053431	55.446	101	45	0	91	191	5	105	7.37e-37	134

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1159270	AT5G17800.1	44.043	277	106	5	86	355	88	322	1.02e-71	226
Msa1159270	AT1G69560.1	46.468	269	98	8	90	356	106	330	5.02e-70	222
Msa1159270	AT1G69560.2	46.468	269	98	8	90	356	122	346	5.66e-70	223
Msa1159270	AT1G26780.2	56.757	185	69	3	88	261	95	279	1.23e-66	214
Msa1159270	AT1G26780.1	72.441	127	35	0	88	214	95	221	1.86e-66	211
Msa1159270	AT1G17950.1	39.777	269	131	5	88	354	2	241	1.50e-61	198
Msa1159270	AT3G29020.1	70.940	117	34	0	86	202	60	176	3.83e-60	194
Msa1159270	AT3G29020.2	70.940	117	34	0	86	202	62	178	1.41e-59	194
Msa1159270	AT4G33450.1	77.358	106	24	0	89	194	17	122	7.39e-59	191
Msa1159270	AT1G73410.1	79.439	107	22	0	88	194	3	109	2.27e-57	187

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Find 90 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCACCCATCTTCTACTT+TGG	0.377594	7_4:+48847914	None:intergenic
AAAGCTAAAGCTAATAGATG+TGG	0.612393	7_4:-48846590	Msa1159270:CDS
AAAGCTAATAGATGTGGTGG+TGG	0.583743	7_4:-48846584	Msa1159270:CDS
AACAGTGTACTGTGTATAGA+AGG	0.441649	7_4:-48847181	Msa1159270:CDS
AAGGAGCTTGTTGCTGAATA+TGG	0.317262	7_4:-48847759	Msa1159270:CDS
AATAGAAGATGCTAAACTTA+AGG	0.419512	7_4:-48847778	Msa1159270:CDS
AATTGAGATATTGGACCTAT+TGG	0.389765	7_4:+48846504	None:intergenic
ACAACTAGTTTAGCTGAAGA+AGG	0.544088	7_4:+48847026	None:intergenic
ACAAGATGAGTTGAAGGAAT+AGG	0.447818	7_4:+48848008	None:intergenic
ACCATCTCAAGCACCATTGA+TGG	0.379437	7_4:-48847083	Msa1159270:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAATGCAGTGAAGAATCAT+TGG	-	chr7_4:48846833-48846852	Msa1159270:intron	30.0%
TGTTGTTGAAAGTCTTTCAA+AGG	-	chr7_4:48846918-48846937	Msa1159270:intron	30.0%
AAAGCTAAAGCTAATAGATG+TGG	-	chr7_4:48847475-48847494	Msa1159270:intron	30.0%
TTGAATCTGAAAAATTGGAC+TGG	+	chr7_4:48847508-48847527	None:intergenic	30.0%
AATTGAGATATTGGACCTAT+TGG	+	chr7_4:48847564-48847583	None:intergenic	30.0%
TTCACCAGAAATTGAGATAT+TGG	+	chr7_4:48847573-48847592	None:intergenic	30.0%
TCTGGTGAAAATGAAAATGT+TGG	-	chr7_4:48847584-48847603	Msa1159270:intron	30.0%
ACTCCAAGAAAATCAATGAA+TGG	+	chr7_4:48847632-48847651	None:intergenic	30.0%
ATAGAACAAACTGTACACAA+GGG	+	chr7_4:48847772-48847791	None:intergenic	30.0%
TATAGAACAAACTGTACACA+AGG	+	chr7_4:48847773-48847792	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_4	gene	48846000	48848087	48846000	ID=Msa1159270;Name=Msa1159270
chr7_4	mRNA	48846000	48848087	48846000	ID=Msa1159270-mRNA-1;Parent=Msa1159270;Name=Msa1159270-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|1\|0.33\|1\|1\|1\|3\|417\|358
chr7_4	exon	48846000	48846697	48846000	ID=Msa1159270-mRNA-1:exon:13296;Parent=Msa1159270-mRNA-1
chr7_4	exon	48846988	48847407	48846988	ID=Msa1159270-mRNA-1:exon:13295;Parent=Msa1159270-mRNA-1
chr7_4	exon	48847712	48848087	48847712	ID=Msa1159270-mRNA-1:exon:13294;Parent=Msa1159270-mRNA-1
chr7_4	CDS	48847712	48848087	48847712	ID=Msa1159270-mRNA-1:cds;Parent=Msa1159270-mRNA-1
chr7_4	CDS	48846988	48847407	48846988	ID=Msa1159270-mRNA-1:cds;Parent=Msa1159270-mRNA-1
chr7_4	CDS	48846417	48846697	48846417	ID=Msa1159270-mRNA-1:cds;Parent=Msa1159270-mRNA-1
chr7_4	three_prime_UTR	48846000	48846416	48846000	ID=Msa1159270-mRNA-1:three_prime_utr;Parent=Msa1159270-mRNA-1

Gene Sequence

>Msa1159270

ATGACTTCACAGAAGTTCAACAATGGTGATATCAATATGAGTCATTTTCCTTTAGCTCCTATTCCTTCAACTCATCTTGTGGATTCCAAAGCTCTTCCTTTGACAAACCCTGAAAAGGGTCTTCAGATTTTGAGACCCTCAATGTTTAGGTCCAAAGTAGAAGATGGGTGCTTTGAGGTTCAAGGAAAAAACCTGTCTTTGAAACTTGGTAATGAAGTAGAAGATAAAAGCTCTGTTTCTGTGAAAGTTGGATACTCCAAGCTTTGTTCTAGAGGTCATTGGAGACCAATAGAAGATGCTAAACTTAAGGAGCTTGTTGCTGAATATGGTCCTCAAAATTGGAACTTGATTGCAGAACATCTTGAAGGAAGATCAGGAAAAAGTTGTAGATTGAGGTGGTTCAATCAGCTAGACCCAAGAATCAACAAAAAAACCTTTTCTGAGGAGGAAGAAGAGAGACTTTTATCTGCTCATAAAATCTATGGTAACAAATGGGCTATAATTGCTAGACTTTTTCCAGGGAGGACAGATAATGCAGTGAAGAATCATTGGCATGTGATCATGGCTAGAAGACATAGGGAACAGTGTACTGTGTATAGAAGGAGAAAGGCTGTTGTTGTTGAAAGTCTTTCAAAGGATTTAAAATTAAGTCTTTCAAACAATGCAGCTAGTGATTCAACCATCTCAAGCACCATTGATGGAAATGTCTCAACTTCCACTAATCTCTCACTCACTCCTTCTTCAGCTAAACTAGTTGTTCCAGTCGAGAGTCATCAAGGCTATGGATCACTAATGGACAAATCTAGAGAAAGTGTGGTGGCAACTAGGGATGTGAATTTTGACAAGTATTTTGGAGCTTGTAAGAAGAATGAACAGGTGGAAAAGCTAAAGCTAATAGATGTGGTGGTGGACCAGTCCAATTTTTCAGATTCAAACTCTGAAGTATCAGTATCTGAGTCAGTTACCACCAATAGGTCCAATATCTCAATTTCTGGTGAAAATGAAAATGTTGGTGTTAAGATTAACATGTTACTACCATTCATTGATTTTCTTGGAGTAGGAGCTATACAAAGCTAA

Protein sequence

>Msa1159270

MTSQKFNNGDINMSHFPLAPIPSTHLVDSKALPLTNPEKGLQILRPSMFRSKVEDGCFEVQGKNLSLKLGNEVEDKSSVSVKVGYSKLCSRGHWRPIEDAKLKELVAEYGPQNWNLIAEHLEGRSGKSCRLRWFNQLDPRINKKTFSEEEEERLLSAHKIYGNKWAIIARLFPGRTDNAVKNHWHVIMARRHREQCTVYRRRKAVVVESLSKDLKLSLSNNAASDSTISSTIDGNVSTSTNLSLTPSSAKLVVPVESHQGYGSLMDKSRESVVATRDVNFDKYFGACKKNEQVEKLKLIDVVVDQSNFSDSNSEVSVSESVTTNRSNISISGENENVGVKINMLLPFIDFLGVGAIQS*