AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1169330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1169330 MtrunA17_Chr7g0263871 100.000 73 0 0 1 73 1 73 7.53e-47 143
Msa1169330 MtrunA17_Chr7g0263831 85.897 78 6 1 1 73 7 84 2.20e-18 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1169330 AT2G37120.1 76.190 63 15 0 11 73 14 76 5.53e-15 63.2
Msa1169330 AT3G53370.1 80.000 60 12 0 14 73 17 76 1.77e-12 57.0

Find 29 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTTGGGACAGGTTATT+TGG 0.128418 7_4:-62304962 Msa1169330:three_prime_UTR
TTGATTGTTCTCCTGGTTAT+TGG 0.231388 7_4:-62305280 Msa1169330:CDS
TAGTACTTTAGCAACATATT+TGG 0.242741 7_4:-62304976 Msa1169330:three_prime_UTR
AGTACTTTAGCAACATATTT+GGG 0.342721 7_4:-62304975 Msa1169330:three_prime_UTR
CCCAGGCTTGATTGTTCTCC+TGG 0.358351 7_4:-62305287 Msa1169330:CDS
CGTTTCAAACCTTTCTCTCT+CGG 0.372437 7_4:-62306530 Msa1169330:five_prime_UTR
TGGGGAAATGTTTAGAATGC+TGG 0.422488 7_4:-62305325 Msa1169330:intron
CGCGGAGAAGCTTCAAGCTA+TGG 0.466693 7_4:+62306468 None:intergenic
GTTTAGAATGCTGGATCTCA+AGG 0.468079 7_4:-62305316 Msa1169330:intron
GACTTGAATCCGAGAGAGAA+AGG 0.468305 7_4:+62306521 None:intergenic
ACCAGGAGAACAATCAAGCC+TGG 0.470447 7_4:+62305286 None:intergenic
ATTGTTCTCCTGGTTATTGG+TGG 0.489076 7_4:-62305277 Msa1169330:CDS
TTGCTGTTGACATTTCTCAT+TGG 0.497549 7_4:-62305253 Msa1169330:CDS
AAGCAAGGAGTCTCTGCACC+TGG 0.518649 7_4:-62305139 Msa1169330:CDS
AAGCTTCTCCGCGTCAACCA+TGG 0.519128 7_4:-62306458 Msa1169330:exon
TTTAGCAACATATTTGGGAC+AGG 0.520962 7_4:-62304970 Msa1169330:three_prime_UTR
AGTTGAAAATCGTAAGAAAG+AGG 0.533668 7_4:+62304913 None:intergenic
GTACAGTGCCTCTACTCTCC+AGG 0.535553 7_4:+62305121 None:intergenic
TCTGTCAATAAAACATAGGA+TGG 0.540133 7_4:+62305017 None:intergenic
TTGTTCTCCTGGTTATTGGT+GGG 0.546594 7_4:-62305276 Msa1169330:CDS
ATTTGGGACAGGTTATTTGG+TGG 0.553725 7_4:-62304959 Msa1169330:three_prime_UTR
ACTTTCTGTCAATAAAACAT+AGG 0.563705 7_4:+62305013 None:intergenic
GCTTTACGTTTATGCACAGA+AGG 0.577488 7_4:-62305221 Msa1169330:CDS
ACAGCAACCCACCAATAACC+AGG 0.598951 7_4:+62305269 None:intergenic
CCAGGAGAACAATCAAGCCT+GGG 0.608678 7_4:+62305287 None:intergenic
GGATCTCAAGGGTTCAACCC+AGG 0.613072 7_4:-62305304 Msa1169330:CDS
TTTAGAATGCTGGATCTCAA+GGG 0.619147 7_4:-62305315 Msa1169330:intron
TTTATCGGCCATGGTTGACG+CGG 0.638815 7_4:+62306450 None:intergenic
TCTCTGCACCTGGAGAGTAG+AGG 0.653251 7_4:-62305129 Msa1169330:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTATTCACATTT+AGG - chr7_4:62305265-62305284 Msa1169330:CDS 10.0%
!!! TTTTTTTTTTATTCACATTT+AGG - chr7_4:62305265-62305284 Msa1169330:CDS 10.0%
!! AAATAAATAATGAAGAATGT+GGG + chr7_4:62305070-62305089 None:intergenic 15.0%
!! AAAATAAATAATGAAGAATG+TGG + chr7_4:62305071-62305090 None:intergenic 15.0%
!! GATCTATTCATAATTAAATT+AGG - chr7_4:62305241-62305260 Msa1169330:CDS 15.0%
!! TTATTTGAGAATAACAATAA+GGG + chr7_4:62305635-62305654 None:intergenic 15.0%
!! CAATAAAAAAATATTTCCAA+AGG + chr7_4:62306044-62306063 None:intergenic 15.0%
!! AAATAAATAATGAAGAATGT+GGG + chr7_4:62305070-62305089 None:intergenic 15.0%
!! AAAATAAATAATGAAGAATG+TGG + chr7_4:62305071-62305090 None:intergenic 15.0%
!! GATCTATTCATAATTAAATT+AGG - chr7_4:62305241-62305260 Msa1169330:CDS 15.0%
!! TTATTTGAGAATAACAATAA+GGG + chr7_4:62305635-62305654 None:intergenic 15.0%
!! CAATAAAAAAATATTTCCAA+AGG + chr7_4:62306044-62306063 None:intergenic 15.0%
!! TGACAAAGAGAAAAAATAAA+CGG + chr7_4:62304945-62304964 None:intergenic 20.0%
!! TTTCAGATCTTAAAAATTCA+TGG - chr7_4:62305090-62305109 Msa1169330:three_prime_UTR 20.0%
!!! TTTTATTCACATTTAGGTTA+AGG - chr7_4:62305271-62305290 Msa1169330:CDS 20.0%
!! CTTATTTGAGAATAACAATA+AGG + chr7_4:62305636-62305655 None:intergenic 20.0%
!! AGATTCTACTTTGAATATAA+TGG - chr7_4:62305858-62305877 Msa1169330:intron 20.0%
!! GATTCTACTTTGAATATAAT+GGG - chr7_4:62305859-62305878 Msa1169330:intron 20.0%
!!! CAAAAAAAAGCACTTAAATA+AGG + chr7_4:62306087-62306106 None:intergenic 20.0%
!! TGACAAAGAGAAAAAATAAA+CGG + chr7_4:62304945-62304964 None:intergenic 20.0%
!! TTTCAGATCTTAAAAATTCA+TGG - chr7_4:62305090-62305109 Msa1169330:three_prime_UTR 20.0%
!!! TTTTATTCACATTTAGGTTA+AGG - chr7_4:62305271-62305290 Msa1169330:CDS 20.0%
!! CTTATTTGAGAATAACAATA+AGG + chr7_4:62305636-62305655 None:intergenic 20.0%
!! AGATTCTACTTTGAATATAA+TGG - chr7_4:62305858-62305877 Msa1169330:intron 20.0%
!! GATTCTACTTTGAATATAAT+GGG - chr7_4:62305859-62305878 Msa1169330:intron 20.0%
!!! CAAAAAAAAGCACTTAAATA+AGG + chr7_4:62306087-62306106 None:intergenic 20.0%
!!! GGAATTGATTTGAAGAAAAT+CGG + chr7_4:62305049-62305068 None:intergenic 25.0%
!!! TGCTTTATTAGACACTTTTA+AGG - chr7_4:62305344-62305363 Msa1169330:intron 25.0%
! AAAATGCATATGATAAAACC+TGG + chr7_4:62305591-62305610 None:intergenic 25.0%
! GGTTACAAATAACATCAATA+TGG + chr7_4:62305614-62305633 None:intergenic 25.0%
!!! TTAATTTGAGAGTGTTTTTG+TGG + chr7_4:62305797-62305816 None:intergenic 25.0%
! CATATTACTATTTGCATACA+TGG - chr7_4:62305976-62305995 Msa1169330:intron 25.0%
!! TTTACATGTCAATTTTCCTT+TGG - chr7_4:62306025-62306044 Msa1169330:intron 25.0%
! CTCAAAAAAGAAGATGAAAA+AGG - chr7_4:62306269-62306288 Msa1169330:intron 25.0%
!!! CCAGAATTTTAAAAAGTACA+GGG + chr7_4:62306370-62306389 None:intergenic 25.0%
!!! ACCAGAATTTTAAAAAGTAC+AGG + chr7_4:62306371-62306390 None:intergenic 25.0%
!!! TTTTTAAAATTCTGGTTCTG+CGG - chr7_4:62306375-62306394 Msa1169330:intron 25.0%
! ACTTTCTGTCAATAAAACAT+AGG + chr7_4:62306429-62306448 None:intergenic 25.0%
! TAGTACTTTAGCAACATATT+TGG - chr7_4:62306463-62306482 Msa1169330:five_prime_UTR 25.0%
! AGTACTTTAGCAACATATTT+GGG - chr7_4:62306464-62306483 Msa1169330:five_prime_UTR 25.0%
!!! GGAATTGATTTGAAGAAAAT+CGG + chr7_4:62305049-62305068 None:intergenic 25.0%
!!! TGCTTTATTAGACACTTTTA+AGG - chr7_4:62305344-62305363 Msa1169330:intron 25.0%
! AAAATGCATATGATAAAACC+TGG + chr7_4:62305591-62305610 None:intergenic 25.0%
! GGTTACAAATAACATCAATA+TGG + chr7_4:62305614-62305633 None:intergenic 25.0%
!!! TTAATTTGAGAGTGTTTTTG+TGG + chr7_4:62305797-62305816 None:intergenic 25.0%
! CATATTACTATTTGCATACA+TGG - chr7_4:62305976-62305995 Msa1169330:intron 25.0%
!! TTTACATGTCAATTTTCCTT+TGG - chr7_4:62306025-62306044 Msa1169330:intron 25.0%
! CTCAAAAAAGAAGATGAAAA+AGG - chr7_4:62306269-62306288 Msa1169330:intron 25.0%
!!! CCAGAATTTTAAAAAGTACA+GGG + chr7_4:62306370-62306389 None:intergenic 25.0%
!!! ACCAGAATTTTAAAAAGTAC+AGG + chr7_4:62306371-62306390 None:intergenic 25.0%
!!! TTTTTAAAATTCTGGTTCTG+CGG - chr7_4:62306375-62306394 Msa1169330:intron 25.0%
! ACTTTCTGTCAATAAAACAT+AGG + chr7_4:62306429-62306448 None:intergenic 25.0%
! TAGTACTTTAGCAACATATT+TGG - chr7_4:62306463-62306482 Msa1169330:five_prime_UTR 25.0%
! AGTACTTTAGCAACATATTT+GGG - chr7_4:62306464-62306483 Msa1169330:five_prime_UTR 25.0%
!!! TAATTTTTTACCTCCATCAC+TGG - chr7_4:62305189-62305208 Msa1169330:CDS 30.0%
!!! AATTTTTTACCTCCATCACT+GGG - chr7_4:62305190-62305209 Msa1169330:CDS 30.0%
TATGAATAGATCCACAAGTA+AGG + chr7_4:62305233-62305252 None:intergenic 30.0%
AACCATTCAACACAAATTGA+TGG + chr7_4:62305566-62305585 None:intergenic 30.0%
ATTCTCAAATAAGTCTGTGT+AGG - chr7_4:62305643-62305662 Msa1169330:intron 30.0%
! TTTTTGTGGAGACATACAAA+AGG + chr7_4:62305783-62305802 None:intergenic 30.0%
ATTGCAGAGCAAATAAAGAA+TGG + chr7_4:62305955-62305974 None:intergenic 30.0%
!!! TGCTTTTTTTTGTTGCTCTT+GGG - chr7_4:62306095-62306114 Msa1169330:intron 30.0%
!!! TTCATCTTCTTTTTTGAGAC+TGG + chr7_4:62306267-62306286 None:intergenic 30.0%
AAAAGAAGATGAAAAAGGAG+AGG - chr7_4:62306274-62306293 Msa1169330:intron 30.0%
!! CTCTAATGTTTTGTTGAATG+TGG - chr7_4:62306340-62306359 Msa1169330:intron 30.0%
!!! CCCTGTACTTTTTAAAATTC+TGG - chr7_4:62306367-62306386 Msa1169330:intron 30.0%
TCTGTCAATAAAACATAGGA+TGG + chr7_4:62306425-62306444 None:intergenic 30.0%
AGTTGAAAATCGTAAGAAAG+AGG + chr7_4:62306529-62306548 None:intergenic 30.0%
!!! TAATTTTTTACCTCCATCAC+TGG - chr7_4:62305189-62305208 Msa1169330:CDS 30.0%
!!! AATTTTTTACCTCCATCACT+GGG - chr7_4:62305190-62305209 Msa1169330:CDS 30.0%
TATGAATAGATCCACAAGTA+AGG + chr7_4:62305233-62305252 None:intergenic 30.0%
AACCATTCAACACAAATTGA+TGG + chr7_4:62305566-62305585 None:intergenic 30.0%
ATTCTCAAATAAGTCTGTGT+AGG - chr7_4:62305643-62305662 Msa1169330:intron 30.0%
! TTTTTGTGGAGACATACAAA+AGG + chr7_4:62305783-62305802 None:intergenic 30.0%
ATTGCAGAGCAAATAAAGAA+TGG + chr7_4:62305955-62305974 None:intergenic 30.0%
!!! TGCTTTTTTTTGTTGCTCTT+GGG - chr7_4:62306095-62306114 Msa1169330:intron 30.0%
!!! TTCATCTTCTTTTTTGAGAC+TGG + chr7_4:62306267-62306286 None:intergenic 30.0%
AAAAGAAGATGAAAAAGGAG+AGG - chr7_4:62306274-62306293 Msa1169330:intron 30.0%
!! CTCTAATGTTTTGTTGAATG+TGG - chr7_4:62306340-62306359 Msa1169330:intron 30.0%
!!! CCCTGTACTTTTTAAAATTC+TGG - chr7_4:62306367-62306386 Msa1169330:intron 30.0%
TCTGTCAATAAAACATAGGA+TGG + chr7_4:62306425-62306444 None:intergenic 30.0%
AGTTGAAAATCGTAAGAAAG+AGG + chr7_4:62306529-62306548 None:intergenic 30.0%
AGAAAATCGGATCTAAGAAG+AGG + chr7_4:62305036-62305055 None:intergenic 35.0%
AGGGCATAGAATATGAGAAA+GGG + chr7_4:62305132-62305151 None:intergenic 35.0%
! AAGGGCATAGAATATGAGAA+AGG + chr7_4:62305133-62305152 None:intergenic 35.0%
! TGAAAGTATGAACGAGCAAA+GGG + chr7_4:62305151-62305170 None:intergenic 35.0%
! TTGAAAGTATGAACGAGCAA+AGG + chr7_4:62305152-62305171 None:intergenic 35.0%
ATAACTGAAAAACCCAGTGA+TGG + chr7_4:62305205-62305224 None:intergenic 35.0%
AGTTATATACGCCTTACTTG+TGG - chr7_4:62305219-62305238 Msa1169330:CDS 35.0%
! GATCATGATGATGATGATGA+TGG - chr7_4:62305457-62305476 Msa1169330:intron 35.0%
! TCAACACAAATTGATGGCAT+TGG + chr7_4:62305560-62305579 None:intergenic 35.0%
TGCCATCAATTTGTGTTGAA+TGG - chr7_4:62305561-62305580 Msa1169330:intron 35.0%
! TTTGTGTTGAATGGTTTTCC+AGG - chr7_4:62305570-62305589 Msa1169330:intron 35.0%
ATTGTATTGTACACGCGTTA+AGG - chr7_4:62305683-62305702 Msa1169330:intron 35.0%
GGAACTGTGAATCATAAATC+AGG + chr7_4:62305762-62305781 None:intergenic 35.0%
! AAGTAGAATCTCTGATTTGC+AGG + chr7_4:62305849-62305868 None:intergenic 35.0%
! TTTGAAACAAAGGAGAGAAG+TGG + chr7_4:62305890-62305909 None:intergenic 35.0%
!! CCAGAGATTCTTTGAAACAA+AGG + chr7_4:62305900-62305919 None:intergenic 35.0%
! CCTTTGTTTCAAAGAATCTC+TGG - chr7_4:62305897-62305916 Msa1169330:intron 35.0%
!!! GTGCTTTTTTTTGTTGCTCT+TGG - chr7_4:62306094-62306113 Msa1169330:intron 35.0%
!!! GCTTTTTTTTGTTGCTCTTG+GGG - chr7_4:62306096-62306115 Msa1169330:intron 35.0%
! TTTAGAATGCTGGATCTCAA+GGG - chr7_4:62306124-62306143 Msa1169330:intron 35.0%
TTGATTGTTCTCCTGGTTAT+TGG - chr7_4:62306159-62306178 Msa1169330:intron 35.0%
! TTGCTGTTGACATTTCTCAT+TGG - chr7_4:62306186-62306205 Msa1169330:intron 35.0%
! TTTAGCAACATATTTGGGAC+AGG - chr7_4:62306469-62306488 Msa1169330:five_prime_UTR 35.0%
! CATATTTGGGACAGGTTATT+TGG - chr7_4:62306477-62306496 Msa1169330:five_prime_UTR 35.0%
AGAAAATCGGATCTAAGAAG+AGG + chr7_4:62305036-62305055 None:intergenic 35.0%
AGGGCATAGAATATGAGAAA+GGG + chr7_4:62305132-62305151 None:intergenic 35.0%
! AAGGGCATAGAATATGAGAA+AGG + chr7_4:62305133-62305152 None:intergenic 35.0%
! TGAAAGTATGAACGAGCAAA+GGG + chr7_4:62305151-62305170 None:intergenic 35.0%
! TTGAAAGTATGAACGAGCAA+AGG + chr7_4:62305152-62305171 None:intergenic 35.0%
ATAACTGAAAAACCCAGTGA+TGG + chr7_4:62305205-62305224 None:intergenic 35.0%
AGTTATATACGCCTTACTTG+TGG - chr7_4:62305219-62305238 Msa1169330:CDS 35.0%
! GATCATGATGATGATGATGA+TGG - chr7_4:62305457-62305476 Msa1169330:intron 35.0%
! TCAACACAAATTGATGGCAT+TGG + chr7_4:62305560-62305579 None:intergenic 35.0%
TGCCATCAATTTGTGTTGAA+TGG - chr7_4:62305561-62305580 Msa1169330:intron 35.0%
! TTTGTGTTGAATGGTTTTCC+AGG - chr7_4:62305570-62305589 Msa1169330:intron 35.0%
ATTGTATTGTACACGCGTTA+AGG - chr7_4:62305683-62305702 Msa1169330:intron 35.0%
GGAACTGTGAATCATAAATC+AGG + chr7_4:62305762-62305781 None:intergenic 35.0%
! AAGTAGAATCTCTGATTTGC+AGG + chr7_4:62305849-62305868 None:intergenic 35.0%
! TTTGAAACAAAGGAGAGAAG+TGG + chr7_4:62305890-62305909 None:intergenic 35.0%
!! CCAGAGATTCTTTGAAACAA+AGG + chr7_4:62305900-62305919 None:intergenic 35.0%
! CCTTTGTTTCAAAGAATCTC+TGG - chr7_4:62305897-62305916 Msa1169330:intron 35.0%
!!! GTGCTTTTTTTTGTTGCTCT+TGG - chr7_4:62306094-62306113 Msa1169330:intron 35.0%
!!! GCTTTTTTTTGTTGCTCTTG+GGG - chr7_4:62306096-62306115 Msa1169330:intron 35.0%
! TTTAGAATGCTGGATCTCAA+GGG - chr7_4:62306124-62306143 Msa1169330:intron 35.0%
TTGATTGTTCTCCTGGTTAT+TGG - chr7_4:62306159-62306178 Msa1169330:intron 35.0%
! TTGCTGTTGACATTTCTCAT+TGG - chr7_4:62306186-62306205 Msa1169330:intron 35.0%
! TTTAGCAACATATTTGGGAC+AGG - chr7_4:62306469-62306488 Msa1169330:five_prime_UTR 35.0%
! CATATTTGGGACAGGTTATT+TGG - chr7_4:62306477-62306496 Msa1169330:five_prime_UTR 35.0%
CGTTTCAAACCTTTCTCTCT+CGG - chr7_4:62304909-62304928 Msa1169330:three_prime_UTR 40.0%
AGAAGAGGAGAGAAGAATAC+CGG + chr7_4:62305021-62305040 None:intergenic 40.0%
GGGCATAGAATATGAGAAAG+GGG + chr7_4:62305131-62305150 None:intergenic 40.0%
! ATGATGGTGATACTGCAGAA+TGG - chr7_4:62305473-62305492 Msa1169330:intron 40.0%
CATACATGGATGATTGTGAG+TGG - chr7_4:62305990-62306009 Msa1169330:intron 40.0%
! TGGGGAAATGTTTAGAATGC+TGG - chr7_4:62306114-62306133 Msa1169330:intron 40.0%
! GTTTAGAATGCTGGATCTCA+AGG - chr7_4:62306123-62306142 Msa1169330:intron 40.0%
ATTGTTCTCCTGGTTATTGG+TGG - chr7_4:62306162-62306181 Msa1169330:intron 40.0%
TTGTTCTCCTGGTTATTGGT+GGG - chr7_4:62306163-62306182 Msa1169330:intron 40.0%
GCTTTACGTTTATGCACAGA+AGG - chr7_4:62306218-62306237 Msa1169330:intron 40.0%
!!! CTGGCTTCTTTTTTCTAGGA+GGG + chr7_4:62306248-62306267 None:intergenic 40.0%
!!! ACTGGCTTCTTTTTTCTAGG+AGG + chr7_4:62306249-62306268 None:intergenic 40.0%
!!! GAGACTGGCTTCTTTTTTCT+AGG + chr7_4:62306252-62306271 None:intergenic 40.0%
! ATTTGGGACAGGTTATTTGG+TGG - chr7_4:62306480-62306499 Msa1169330:five_prime_UTR 40.0%
CGTTTCAAACCTTTCTCTCT+CGG - chr7_4:62304909-62304928 Msa1169330:three_prime_UTR 40.0%
AGAAGAGGAGAGAAGAATAC+CGG + chr7_4:62305021-62305040 None:intergenic 40.0%
GGGCATAGAATATGAGAAAG+GGG + chr7_4:62305131-62305150 None:intergenic 40.0%
! ATGATGGTGATACTGCAGAA+TGG - chr7_4:62305473-62305492 Msa1169330:intron 40.0%
CATACATGGATGATTGTGAG+TGG - chr7_4:62305990-62306009 Msa1169330:intron 40.0%
! TGGGGAAATGTTTAGAATGC+TGG - chr7_4:62306114-62306133 Msa1169330:intron 40.0%
! GTTTAGAATGCTGGATCTCA+AGG - chr7_4:62306123-62306142 Msa1169330:intron 40.0%
ATTGTTCTCCTGGTTATTGG+TGG - chr7_4:62306162-62306181 Msa1169330:intron 40.0%
TTGTTCTCCTGGTTATTGGT+GGG - chr7_4:62306163-62306182 Msa1169330:intron 40.0%
GCTTTACGTTTATGCACAGA+AGG - chr7_4:62306218-62306237 Msa1169330:intron 40.0%
!!! CTGGCTTCTTTTTTCTAGGA+GGG + chr7_4:62306248-62306267 None:intergenic 40.0%
!!! ACTGGCTTCTTTTTTCTAGG+AGG + chr7_4:62306249-62306268 None:intergenic 40.0%
!!! GAGACTGGCTTCTTTTTTCT+AGG + chr7_4:62306252-62306271 None:intergenic 40.0%
! ATTTGGGACAGGTTATTTGG+TGG - chr7_4:62306480-62306499 Msa1169330:five_prime_UTR 40.0%
GACTTGAATCCGAGAGAGAA+AGG + chr7_4:62304921-62304940 None:intergenic 45.0%
AAGAGGAGAGAAGAATACCG+GGG + chr7_4:62305019-62305038 None:intergenic 45.0%
GAAGAGGAGAGAAGAATACC+GGG + chr7_4:62305020-62305039 None:intergenic 45.0%
! TATGAGAAAGGGGCATGCAA+GGG + chr7_4:62305121-62305140 None:intergenic 45.0%
! ATATGAGAAAGGGGCATGCA+AGG + chr7_4:62305122-62305141 None:intergenic 45.0%
ACTGAAAAACCCAGTGATGG+AGG + chr7_4:62305202-62305221 None:intergenic 45.0%
ACATGGATGATTGTGAGTGG+TGG - chr7_4:62305993-62306012 Msa1169330:intron 45.0%
AAAAAGGAGAGGCTGAAGCA+AGG - chr7_4:62306285-62306304 Msa1169330:intron 45.0%
GACTTGAATCCGAGAGAGAA+AGG + chr7_4:62304921-62304940 None:intergenic 45.0%
AAGAGGAGAGAAGAATACCG+GGG + chr7_4:62305019-62305038 None:intergenic 45.0%
GAAGAGGAGAGAAGAATACC+GGG + chr7_4:62305020-62305039 None:intergenic 45.0%
! TATGAGAAAGGGGCATGCAA+GGG + chr7_4:62305121-62305140 None:intergenic 45.0%
! ATATGAGAAAGGGGCATGCA+AGG + chr7_4:62305122-62305141 None:intergenic 45.0%
ACTGAAAAACCCAGTGATGG+AGG + chr7_4:62305202-62305221 None:intergenic 45.0%
ACATGGATGATTGTGAGTGG+TGG - chr7_4:62305993-62306012 Msa1169330:intron 45.0%
AAAAAGGAGAGGCTGAAGCA+AGG - chr7_4:62306285-62306304 Msa1169330:intron 45.0%
TTTATCGGCCATGGTTGACG+CGG + chr7_4:62304992-62305011 None:intergenic 50.0%
GAATACCGGGGGGACTTTAT+CGG + chr7_4:62305007-62305026 None:intergenic 50.0%
AGAGGAGAGAAGAATACCGG+GGG + chr7_4:62305018-62305037 None:intergenic 50.0%
CCAGGAGAACAATCAAGCCT+GGG + chr7_4:62306155-62306174 None:intergenic 50.0%
ACCAGGAGAACAATCAAGCC+TGG + chr7_4:62306156-62306175 None:intergenic 50.0%
ACAGCAACCCACCAATAACC+AGG + chr7_4:62306173-62306192 None:intergenic 50.0%
TTTATCGGCCATGGTTGACG+CGG + chr7_4:62304992-62305011 None:intergenic 50.0%
GAATACCGGGGGGACTTTAT+CGG + chr7_4:62305007-62305026 None:intergenic 50.0%
AGAGGAGAGAAGAATACCGG+GGG + chr7_4:62305018-62305037 None:intergenic 50.0%
CCAGGAGAACAATCAAGCCT+GGG + chr7_4:62306155-62306174 None:intergenic 50.0%
ACCAGGAGAACAATCAAGCC+TGG + chr7_4:62306156-62306175 None:intergenic 50.0%
ACAGCAACCCACCAATAACC+AGG + chr7_4:62306173-62306192 None:intergenic 50.0%
CGCGGAGAAGCTTCAAGCTA+TGG + chr7_4:62304974-62304993 None:intergenic 55.0%
AAGCTTCTCCGCGTCAACCA+TGG - chr7_4:62304981-62305000 Msa1169330:three_prime_UTR 55.0%
GAGGAGAGAAGAATACCGGG+GGG + chr7_4:62305017-62305036 None:intergenic 55.0%
GGATCTCAAGGGTTCAACCC+AGG - chr7_4:62306135-62306154 Msa1169330:intron 55.0%
CCCAGGCTTGATTGTTCTCC+TGG - chr7_4:62306152-62306171 Msa1169330:intron 55.0%
AAGCAAGGAGTCTCTGCACC+TGG - chr7_4:62306300-62306319 Msa1169330:intron 55.0%
TCTCTGCACCTGGAGAGTAG+AGG - chr7_4:62306310-62306329 Msa1169330:intron 55.0%
GTACAGTGCCTCTACTCTCC+AGG + chr7_4:62306321-62306340 None:intergenic 55.0%
CGCGGAGAAGCTTCAAGCTA+TGG + chr7_4:62304974-62304993 None:intergenic 55.0%
AAGCTTCTCCGCGTCAACCA+TGG - chr7_4:62304981-62305000 Msa1169330:three_prime_UTR 55.0%
GAGGAGAGAAGAATACCGGG+GGG + chr7_4:62305017-62305036 None:intergenic 55.0%
GGATCTCAAGGGTTCAACCC+AGG - chr7_4:62306135-62306154 Msa1169330:intron 55.0%
CCCAGGCTTGATTGTTCTCC+TGG - chr7_4:62306152-62306171 Msa1169330:intron 55.0%
AAGCAAGGAGTCTCTGCACC+TGG - chr7_4:62306300-62306319 Msa1169330:intron 55.0%
TCTCTGCACCTGGAGAGTAG+AGG - chr7_4:62306310-62306329 Msa1169330:intron 55.0%
GTACAGTGCCTCTACTCTCC+AGG + chr7_4:62306321-62306340 None:intergenic 55.0%
CATGGCCGATAAAGTCCCCC+CGG - chr7_4:62304999-62305018 Msa1169330:three_prime_UTR 60.0%
CATGGCCGATAAAGTCCCCC+CGG - chr7_4:62304999-62305018 Msa1169330:three_prime_UTR 60.0%
CGGGGGGACTTTATCGGCCA+TGG + chr7_4:62305001-62305020 None:intergenic 65.0%
CGGGGGGACTTTATCGGCCA+TGG + chr7_4:62305001-62305020 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 62304887 62306574 62304887 ID=Msa1169330;Name=Msa1169330
chr7_4 mRNA 62304887 62306574 62304887 ID=Msa1169330-mRNA-1;Parent=Msa1169330;Name=Msa1169330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=113|1|1|1|1|1|2|245|73
chr7_4 exon 62304887 62305332 62304887 ID=Msa1169330-mRNA-1:exon:19736;Parent=Msa1169330-mRNA-1
chr7_4 exon 62306441 62306574 62306441 ID=Msa1169330-mRNA-1:exon:19735;Parent=Msa1169330-mRNA-1
chr7_4 five_prime_UTR 62306462 62306574 62306462 ID=Msa1169330-mRNA-1:five_prime_utr;Parent=Msa1169330-mRNA-1
chr7_4 CDS 62306441 62306461 62306441 ID=Msa1169330-mRNA-1:cds;Parent=Msa1169330-mRNA-1
chr7_4 CDS 62305132 62305332 62305132 ID=Msa1169330-mRNA-1:cds;Parent=Msa1169330-mRNA-1
chr7_4 three_prime_UTR 62304887 62305131 62304887 ID=Msa1169330-mRNA-1:three_prime_utr;Parent=Msa1169330-mRNA-1
Gene Sequence

>Msa1169330

ATGGCCGATAAAGTCCCCCCGAATGCTGGATCTCAAGGGTTCAACCCAGGCTTGATTGTTCTCCTGGTTATTGGTGGGTTGCTGTTGACATTTCTCATTGGAAATTATGCGCTTTACGTTTATGCACAGAAGGCTCTCCCTCCTAGAAAAAAGAAGCCAGTCTCAAAAAAGAAGATGAAAAAGGAGAGGCTGAAGCAAGGAGTCTCTGCACCTGGAGAGTAG
Protein sequence

>Msa1169330

MADKVPPNAGSQGFNPGLIVLLVIGGLLLTFLIGNYALYVYAQKALPPRKKKPVSKKKMKKERLKQGVSAPGE*