AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1171960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171960 MtrunA17_Chr4g0059321 99.487 195 1 0 1 195 396 590 8.19e-139 400
Msa1171960 MtrunA17_Chr6g0448981 43.523 193 108 1 1 193 346 537 1.59e-49 168
Msa1171960 MtrunA17_Chr1g0196111 39.378 193 113 1 1 193 242 430 1.53e-40 143
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171960 AT5G40200.1 80.513 195 38 0 1 195 394 588 3.27e-105 314
Msa1171960 AT5G36950.1 43.814 194 107 2 1 193 378 570 8.87e-45 156
Msa1171960 AT2G47940.2 40.933 193 110 1 1 193 382 570 7.46e-42 149
Msa1171960 AT2G47940.1 40.933 193 110 1 1 193 383 571 7.53e-42 149
Msa1171960 AT1G65630.1 38.342 193 108 1 2 194 361 542 4.07e-32 121
Msa1171960 AT1G65640.2 39.572 187 97 2 2 188 339 509 6.36e-32 120
Msa1171960 AT3G16540.1 36.269 193 97 1 1 193 364 530 8.54e-29 112
Msa1171960 AT3G16550.1 35.294 187 101 1 6 192 333 499 1.01e-25 103
Msa1171960 AT5G40560.1 35.789 190 98 3 6 195 309 474 3.03e-23 96.3
Msa1171960 AT1G65640.1 35.780 109 59 1 2 110 339 436 6.20e-13 66.6

Find 32 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGCTCCAGTCAAGATTT+TGG 0.209977 8_1:+1890836 Msa1171960:CDS
CCCTCATATTATATCATTGC+AGG 0.292872 8_1:+1890483 Msa1171960:CDS
AAATTCAATATTAAACTTGA+TGG 0.313467 8_1:+1890420 Msa1171960:CDS
GGCTTGCCCTTGCTGTGTGC+TGG 0.318695 8_1:-1890459 None:intergenic
CTCATATCACAGAAATATAC+TGG 0.394005 8_1:+1890354 Msa1171960:CDS
TTAAACTTGATGGTCATAGA+AGG 0.395091 8_1:+1890430 Msa1171960:CDS
ATTACAACAAGTTGTTCATC+TGG 0.408369 8_1:-1890888 None:intergenic
TCCTGCAATGATATAATATG+AGG 0.430469 8_1:-1890484 None:intergenic
ATGCGCTCTCCATGGCGAAA+TGG 0.455675 8_1:-1890318 None:intergenic
TCTGTATTTCTGAAGGTCGT+TGG 0.456981 8_1:+1891145 Msa1171960:intron
ACAACTTGTTGTAATTTCTC+AGG 0.458392 8_1:+1890896 Msa1171960:CDS
CAAACCTCAGAACGAAGATA+AGG 0.514741 8_1:-1890531 None:intergenic
TCTTAAGAGCTTGGCCGCAA+TGG 0.525473 8_1:+1891192 Msa1171960:CDS
ATTTGATTTAGATTATGAGC+AGG 0.539800 8_1:+1891243 Msa1171960:CDS
GGTTCCTTATCTTCGTTCTG+AGG 0.546360 8_1:+1890527 Msa1171960:CDS
TTTCGCCATGGAGAGCGCAT+TGG 0.546950 8_1:+1890321 Msa1171960:CDS
AGTTTATCCAAAATCTTGAC+TGG 0.552356 8_1:-1890843 None:intergenic
AGATCGTCAGACATTGCTGA+TGG 0.570063 8_1:-1891412 Msa1171970:three_prime_UTR
GCTTATTCCAGCACACAGCA+AGG 0.586438 8_1:+1890452 Msa1171960:CDS
TGAAACCAATGCGCTCTCCA+TGG 0.591141 8_1:-1890326 None:intergenic
CCTGCAATGATATAATATGA+GGG 0.598553 8_1:-1890483 None:intergenic
AGTTGTTCATCTGGTGATTG+TGG 0.604082 8_1:-1890879 None:intergenic
TTTCCAGTTCCATTTCGCCA+TGG 0.606699 8_1:+1890309 None:intergenic
CTTATTCCAGCACACAGCAA+GGG 0.624783 8_1:+1890453 Msa1171960:CDS
TTGACACATATAGGTGCTGG+TGG 0.631847 8_1:+1890989 Msa1171960:intron
CATGTATAAACAGTACGGGA+AGG 0.634604 8_1:+1890803 Msa1171960:intron
ATCACAGCTCTCTACCATTG+CGG 0.652168 8_1:-1891206 None:intergenic
ATGCAGCAATCAAAGTATTG+CGG 0.658146 8_1:+1890382 Msa1171960:CDS
TGAAGAGATCGTTAACACCC+AGG 0.659316 8_1:+1891031 Msa1171960:CDS
CTGGTGGCTGATATTAACAT+CGG 0.661404 8_1:+1891005 Msa1171960:CDS
TGTTGCAAGAATATCTAGCG+TGG 0.690556 8_1:-1891380 Msa1171970:three_prime_UTR
GCAATGATATAATATGAGGG+AGG 0.693321 8_1:-1890480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATATTTTACTTTACTTT+TGG + chr8_1:1890706-1890725 Msa1171960:intron 10.0%
!!! TTTATATTTTACTTTACTTT+TGG + chr8_1:1890706-1890725 Msa1171960:intron 10.0%
!! AAATTCAATATTAAACTTGA+TGG + chr8_1:1890420-1890439 Msa1171960:CDS 15.0%
!! AAATTCAATATTAAACTTGA+TGG + chr8_1:1890420-1890439 Msa1171960:CDS 15.0%
!! AAATTGAGTTAGAAAAGAAT+GGG - chr8_1:1890781-1890800 None:intergenic 20.0%
!! AAAATTGAGTTAGAAAAGAA+TGG - chr8_1:1890782-1890801 None:intergenic 20.0%
!!! TTTTTCATGTATAAACAGTA+CGG + chr8_1:1890798-1890817 Msa1171960:intron 20.0%
!! AAATTGAGTTAGAAAAGAAT+GGG - chr8_1:1890781-1890800 None:intergenic 20.0%
!! AAAATTGAGTTAGAAAAGAA+TGG - chr8_1:1890782-1890801 None:intergenic 20.0%
!!! TTTTTCATGTATAAACAGTA+CGG + chr8_1:1890798-1890817 Msa1171960:intron 20.0%
!!! ATTTGTTTTTTCAACGGTTT+CGG + chr8_1:1890506-1890525 Msa1171960:CDS 25.0%
!!! ATTAGTTTTTCGTAGAATTC+TGG + chr8_1:1890561-1890580 Msa1171960:intron 25.0%
! ATATAAAGCTGCATATTACA+AGG - chr8_1:1890667-1890686 None:intergenic 25.0%
! ATATAAAGCTGCATATAACA+AGG - chr8_1:1890693-1890712 None:intergenic 25.0%
!! TTTTCATGTATAAACAGTAC+GGG + chr8_1:1890799-1890818 Msa1171960:intron 25.0%
!!! CATATTTTTGTTTTCCATGT+TGG + chr8_1:1891072-1891091 Msa1171960:intron 25.0%
!!! AAGCTCTTAAGATTTTTTAC+AGG - chr8_1:1891184-1891203 None:intergenic 25.0%
! TGTAAAAAATCTTAAGAGCT+TGG + chr8_1:1891183-1891202 Msa1171960:CDS 25.0%
!! ATTTGATTTAGATTATGAGC+AGG + chr8_1:1891243-1891262 Msa1171960:CDS 25.0%
!!! ATTTGTTTTTTCAACGGTTT+CGG + chr8_1:1890506-1890525 Msa1171960:CDS 25.0%
!!! ATTAGTTTTTCGTAGAATTC+TGG + chr8_1:1890561-1890580 Msa1171960:intron 25.0%
! ATATAAAGCTGCATATTACA+AGG - chr8_1:1890667-1890686 None:intergenic 25.0%
! ATATAAAGCTGCATATAACA+AGG - chr8_1:1890693-1890712 None:intergenic 25.0%
!! TTTTCATGTATAAACAGTAC+GGG + chr8_1:1890799-1890818 Msa1171960:intron 25.0%
!!! CATATTTTTGTTTTCCATGT+TGG + chr8_1:1891072-1891091 Msa1171960:intron 25.0%
!!! AAGCTCTTAAGATTTTTTAC+AGG - chr8_1:1891184-1891203 None:intergenic 25.0%
! TGTAAAAAATCTTAAGAGCT+TGG + chr8_1:1891183-1891202 Msa1171960:CDS 25.0%
!! ATTTGATTTAGATTATGAGC+AGG + chr8_1:1891243-1891262 Msa1171960:CDS 25.0%
CTCATATCACAGAAATATAC+TGG + chr8_1:1890354-1890373 Msa1171960:CDS 30.0%
! TTAAACTTGATGGTCATAGA+AGG + chr8_1:1890430-1890449 Msa1171960:CDS 30.0%
CCTGCAATGATATAATATGA+GGG - chr8_1:1890486-1890505 None:intergenic 30.0%
TCCTGCAATGATATAATATG+AGG - chr8_1:1890487-1890506 None:intergenic 30.0%
!!! TGCAGGATTTGTTTTTTCAA+CGG + chr8_1:1890500-1890519 Msa1171960:CDS 30.0%
AGTTTATCCAAAATCTTGAC+TGG - chr8_1:1890846-1890865 None:intergenic 30.0%
ATTACAACAAGTTGTTCATC+TGG - chr8_1:1890891-1890910 None:intergenic 30.0%
!! ACAACTTGTTGTAATTTCTC+AGG + chr8_1:1890896-1890915 Msa1171960:CDS 30.0%
AGAGATACTCAGATACAATA+AGG - chr8_1:1890969-1890988 None:intergenic 30.0%
AGTATCTCTTTGACACATAT+AGG + chr8_1:1890980-1890999 Msa1171960:intron 30.0%
GACAGATTGAGAAATAAAGA+CGG - chr8_1:1891270-1891289 None:intergenic 30.0%
CTCATATCACAGAAATATAC+TGG + chr8_1:1890354-1890373 Msa1171960:CDS 30.0%
! TTAAACTTGATGGTCATAGA+AGG + chr8_1:1890430-1890449 Msa1171960:CDS 30.0%
CCTGCAATGATATAATATGA+GGG - chr8_1:1890486-1890505 None:intergenic 30.0%
TCCTGCAATGATATAATATG+AGG - chr8_1:1890487-1890506 None:intergenic 30.0%
!!! TGCAGGATTTGTTTTTTCAA+CGG + chr8_1:1890500-1890519 Msa1171960:CDS 30.0%
AGTTTATCCAAAATCTTGAC+TGG - chr8_1:1890846-1890865 None:intergenic 30.0%
ATTACAACAAGTTGTTCATC+TGG - chr8_1:1890891-1890910 None:intergenic 30.0%
!! ACAACTTGTTGTAATTTCTC+AGG + chr8_1:1890896-1890915 Msa1171960:CDS 30.0%
AGAGATACTCAGATACAATA+AGG - chr8_1:1890969-1890988 None:intergenic 30.0%
AGTATCTCTTTGACACATAT+AGG + chr8_1:1890980-1890999 Msa1171960:intron 30.0%
GACAGATTGAGAAATAAAGA+CGG - chr8_1:1891270-1891289 None:intergenic 30.0%
!! ATGCAGCAATCAAAGTATTG+CGG + chr8_1:1890382-1890401 Msa1171960:CDS 35.0%
GCAATGATATAATATGAGGG+AGG - chr8_1:1890483-1890502 None:intergenic 35.0%
CCCTCATATTATATCATTGC+AGG + chr8_1:1890483-1890502 Msa1171960:CDS 35.0%
! ACAATATGCTTGCTAGAAGT+TGG + chr8_1:1890603-1890622 Msa1171960:intron 35.0%
! CAATATGCTTGCTAGAAGTT+GGG + chr8_1:1890604-1890623 Msa1171960:intron 35.0%
AAATTCGCTAAGCTTTCTTG+TGG + chr8_1:1890754-1890773 Msa1171960:intron 35.0%
TACTCAGCAAAAACAGCAAA+AGG - chr8_1:1890946-1890965 None:intergenic 35.0%
AACATTGCTCATATCAACCT+GGG - chr8_1:1891051-1891070 None:intergenic 35.0%
AAACATTGCTCATATCAACC+TGG - chr8_1:1891052-1891071 None:intergenic 35.0%
! TCTACTCTCTGTATTTCTGA+AGG + chr8_1:1891138-1891157 Msa1171960:intron 35.0%
CTAATCATCTATGCAGATAG+TGG + chr8_1:1891330-1891349 Msa1171960:intron 35.0%
!! ATGCAGCAATCAAAGTATTG+CGG + chr8_1:1890382-1890401 Msa1171960:CDS 35.0%
GCAATGATATAATATGAGGG+AGG - chr8_1:1890483-1890502 None:intergenic 35.0%
CCCTCATATTATATCATTGC+AGG + chr8_1:1890483-1890502 Msa1171960:CDS 35.0%
! ACAATATGCTTGCTAGAAGT+TGG + chr8_1:1890603-1890622 Msa1171960:intron 35.0%
! CAATATGCTTGCTAGAAGTT+GGG + chr8_1:1890604-1890623 Msa1171960:intron 35.0%
AAATTCGCTAAGCTTTCTTG+TGG + chr8_1:1890754-1890773 Msa1171960:intron 35.0%
TACTCAGCAAAAACAGCAAA+AGG - chr8_1:1890946-1890965 None:intergenic 35.0%
AACATTGCTCATATCAACCT+GGG - chr8_1:1891051-1891070 None:intergenic 35.0%
AAACATTGCTCATATCAACC+TGG - chr8_1:1891052-1891071 None:intergenic 35.0%
! TCTACTCTCTGTATTTCTGA+AGG + chr8_1:1891138-1891157 Msa1171960:intron 35.0%
CTAATCATCTATGCAGATAG+TGG + chr8_1:1891330-1891349 Msa1171960:intron 35.0%
CAAACCTCAGAACGAAGATA+AGG - chr8_1:1890534-1890553 None:intergenic 40.0%
CTTCTAGCAAGCATATTGTG+AGG - chr8_1:1890602-1890621 None:intergenic 40.0%
CATGTATAAACAGTACGGGA+AGG + chr8_1:1890803-1890822 Msa1171960:intron 40.0%
! TGAAGCTCCAGTCAAGATTT+TGG + chr8_1:1890836-1890855 Msa1171960:CDS 40.0%
AGTTGTTCATCTGGTGATTG+TGG - chr8_1:1890882-1890901 None:intergenic 40.0%
TCTTTGACACATATAGGTGC+TGG + chr8_1:1890986-1891005 Msa1171960:intron 40.0%
CTGGTGGCTGATATTAACAT+CGG + chr8_1:1891005-1891024 Msa1171960:CDS 40.0%
! TCTGTATTTCTGAAGGTCGT+TGG + chr8_1:1891145-1891164 Msa1171960:intron 40.0%
!!! CTGAAGGTCGTTGGTTTTAA+TGG + chr8_1:1891154-1891173 Msa1171960:intron 40.0%
GTCTTGCGTGCTTAGAAAAA+TGG + chr8_1:1891287-1891306 Msa1171960:intron 40.0%
TGTTGCAAGAATATCTAGCG+TGG - chr8_1:1891383-1891402 Msa1171970:three_prime_UTR 40.0%
CAAACCTCAGAACGAAGATA+AGG - chr8_1:1890534-1890553 None:intergenic 40.0%
CTTCTAGCAAGCATATTGTG+AGG - chr8_1:1890602-1890621 None:intergenic 40.0%
CATGTATAAACAGTACGGGA+AGG + chr8_1:1890803-1890822 Msa1171960:intron 40.0%
! TGAAGCTCCAGTCAAGATTT+TGG + chr8_1:1890836-1890855 Msa1171960:CDS 40.0%
AGTTGTTCATCTGGTGATTG+TGG - chr8_1:1890882-1890901 None:intergenic 40.0%
TCTTTGACACATATAGGTGC+TGG + chr8_1:1890986-1891005 Msa1171960:intron 40.0%
CTGGTGGCTGATATTAACAT+CGG + chr8_1:1891005-1891024 Msa1171960:CDS 40.0%
! TCTGTATTTCTGAAGGTCGT+TGG + chr8_1:1891145-1891164 Msa1171960:intron 40.0%
!!! CTGAAGGTCGTTGGTTTTAA+TGG + chr8_1:1891154-1891173 Msa1171960:intron 40.0%
GTCTTGCGTGCTTAGAAAAA+TGG + chr8_1:1891287-1891306 Msa1171960:intron 40.0%
TGTTGCAAGAATATCTAGCG+TGG - chr8_1:1891383-1891402 Msa1171970:three_prime_UTR 40.0%
CTTATTCCAGCACACAGCAA+GGG + chr8_1:1890453-1890472 Msa1171960:CDS 45.0%
GGTTCCTTATCTTCGTTCTG+AGG + chr8_1:1890527-1890546 Msa1171960:CDS 45.0%
!! TTGACACATATAGGTGCTGG+TGG + chr8_1:1890989-1891008 Msa1171960:intron 45.0%
TGAAGAGATCGTTAACACCC+AGG + chr8_1:1891031-1891050 Msa1171960:CDS 45.0%
AGAGTTATCAGCGTCCAACA+TGG - chr8_1:1891089-1891108 None:intergenic 45.0%
TGTTGGACGCTGATAACTCT+TGG + chr8_1:1891089-1891108 Msa1171960:intron 45.0%
ATCACAGCTCTCTACCATTG+CGG - chr8_1:1891209-1891228 None:intergenic 45.0%
AGATCGTCAGACATTGCTGA+TGG - chr8_1:1891415-1891434 Msa1171970:three_prime_UTR 45.0%
CTTATTCCAGCACACAGCAA+GGG + chr8_1:1890453-1890472 Msa1171960:CDS 45.0%
GGTTCCTTATCTTCGTTCTG+AGG + chr8_1:1890527-1890546 Msa1171960:CDS 45.0%
!! TTGACACATATAGGTGCTGG+TGG + chr8_1:1890989-1891008 Msa1171960:intron 45.0%
TGAAGAGATCGTTAACACCC+AGG + chr8_1:1891031-1891050 Msa1171960:CDS 45.0%
AGAGTTATCAGCGTCCAACA+TGG - chr8_1:1891089-1891108 None:intergenic 45.0%
TGTTGGACGCTGATAACTCT+TGG + chr8_1:1891089-1891108 Msa1171960:intron 45.0%
ATCACAGCTCTCTACCATTG+CGG - chr8_1:1891209-1891228 None:intergenic 45.0%
AGATCGTCAGACATTGCTGA+TGG - chr8_1:1891415-1891434 Msa1171970:three_prime_UTR 45.0%
TGAAACCAATGCGCTCTCCA+TGG - chr8_1:1890329-1890348 None:intergenic 50.0%
GCTTATTCCAGCACACAGCA+AGG + chr8_1:1890452-1890471 Msa1171960:CDS 50.0%
TCTTAAGAGCTTGGCCGCAA+TGG + chr8_1:1891192-1891211 Msa1171960:CDS 50.0%
! CTTTTGCAGTCTTCGTGCGA+AGG - chr8_1:1891357-1891376 Msa1171970:three_prime_UTR 50.0%
TGAAACCAATGCGCTCTCCA+TGG - chr8_1:1890329-1890348 None:intergenic 50.0%
GCTTATTCCAGCACACAGCA+AGG + chr8_1:1890452-1890471 Msa1171960:CDS 50.0%
TCTTAAGAGCTTGGCCGCAA+TGG + chr8_1:1891192-1891211 Msa1171960:CDS 50.0%
! CTTTTGCAGTCTTCGTGCGA+AGG - chr8_1:1891357-1891376 Msa1171970:three_prime_UTR 50.0%
ATGCGCTCTCCATGGCGAAA+TGG - chr8_1:1890321-1890340 None:intergenic 55.0%
! TTTCGCCATGGAGAGCGCAT+TGG + chr8_1:1890321-1890340 Msa1171960:CDS 55.0%
ATGCGCTCTCCATGGCGAAA+TGG - chr8_1:1890321-1890340 None:intergenic 55.0%
! TTTCGCCATGGAGAGCGCAT+TGG + chr8_1:1890321-1890340 Msa1171960:CDS 55.0%
! GGCTTGCCCTTGCTGTGTGC+TGG - chr8_1:1890462-1890481 None:intergenic 65.0%
! GGCTTGCCCTTGCTGTGTGC+TGG - chr8_1:1890462-1890481 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 1890318 1891444 1890318 ID=Msa1171960;Name=Msa1171960
chr8_1 mRNA 1890318 1891444 1890318 ID=Msa1171960-mRNA-1;Parent=Msa1171960;Name=Msa1171960-mRNA-1;_AED=0.52;_eAED=0.52;_QI=0|0.25|0|0.4|1|0.8|5|0|195
chr8_1 exon 1890318 1890548 1890318 ID=Msa1171960-mRNA-1:exon:726;Parent=Msa1171960-mRNA-1
chr8_1 exon 1890816 1890917 1890816 ID=Msa1171960-mRNA-1:exon:727;Parent=Msa1171960-mRNA-1
chr8_1 exon 1891002 1891052 1891002 ID=Msa1171960-mRNA-1:exon:728;Parent=Msa1171960-mRNA-1
chr8_1 exon 1891160 1891264 1891160 ID=Msa1171960-mRNA-1:exon:729;Parent=Msa1171960-mRNA-1
chr8_1 exon 1891346 1891444 1891346 ID=Msa1171960-mRNA-1:exon:730;Parent=Msa1171960-mRNA-1
chr8_1 CDS 1890318 1890548 1890318 ID=Msa1171960-mRNA-1:cds;Parent=Msa1171960-mRNA-1
chr8_1 CDS 1890816 1890917 1890816 ID=Msa1171960-mRNA-1:cds;Parent=Msa1171960-mRNA-1
chr8_1 CDS 1891002 1891052 1891002 ID=Msa1171960-mRNA-1:cds;Parent=Msa1171960-mRNA-1
chr8_1 CDS 1891160 1891264 1891160 ID=Msa1171960-mRNA-1:cds;Parent=Msa1171960-mRNA-1
chr8_1 CDS 1891346 1891444 1891346 ID=Msa1171960-mRNA-1:cds;Parent=Msa1171960-mRNA-1
Gene Sequence

>Msa1171960

CCATTTCGCCATGGAGAGCGCATTGGTTTCAGCTATCTCATATCACAGAAATATACTGGCGATGATGCAGCAATCAAAGTATTGCGGAATTCAAATGTTCTCAAATTCAATATTAAACTTGATGGTCATAGAAGGCTTATTCCAGCACACAGCAAGGGCAAGCCTCCCTCATATTATATCATTGCAGGATTTGTTTTTTCAACGGTTTCGGTTCCTTATCTTCGTTCTGAGTACGGGAAGGACTATGAATATGAAGCTCCAGTCAAGATTTTGGATAAACTGCTGTATGCAATGCCACAATCACCAGATGAACAACTTGTTGTAATTTCTCAGGTGCTGGTGGCTGATATTAACATCGGATATGAAGAGATCGTTAACACCCAGGTCGTTGGTTTTAATGGTAAACCTGTAAAAAATCTTAAGAGCTTGGCCGCAATGGTAGAGAGCTGTGATGATGAGTATCTGAAATTTGATTTAGATTATGAGCAGATAGTGGTCCTTCGCACGAAGACTGCAAAAGCTGCCACGCTAGATATTCTTGCAACACACTGCATTCCATCAGCAATGTCTGACGATCTCAAGTCATAG

Protein sequence

>Msa1171960

PFRHGERIGFSYLISQKYTGDDAAIKVLRNSNVLKFNIKLDGHRRLIPAHSKGKPPSYYIIAGFVFSTVSVPYLRSEYGKDYEYEAPVKILDKLLYAMPQSPDEQLVVISQVLVADINIGYEEIVNTQVVGFNGKPVKNLKSLAAMVESCDDEYLKFDLDYEQIVVLRTKTAKAATLDILATHCIPSAMSDDLKS*