Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867060 | KEH32495.1 | 99.516 | 413 | 2 | 0 | 1 | 413 | 1 | 413 | 0.0 | 850 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867060 | sp|Q40468|IF415_TOBAC | 92.494 | 413 | 31 | 0 | 1 | 413 | 1 | 413 | 0.0 | 792 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867060 | A0A072V2Y5 | 99.516 | 413 | 2 | 0 | 1 | 413 | 1 | 413 | 0.0 | 850 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002750 | Msa0867060 | 0.807859 | 4.115116e-50 | -8.615850e-47 |
| Msa0002790 | Msa0867060 | 0.812230 | 4.706692e-51 | -8.615850e-47 |
| Msa0003780 | Msa0867060 | 0.813963 | 1.961392e-51 | -8.615850e-47 |
| Msa0022970 | Msa0867060 | 0.809293 | 2.032638e-50 | -8.615850e-47 |
| Msa0032780 | Msa0867060 | 0.825934 | 3.574875e-54 | -8.615850e-47 |
| Msa0037410 | Msa0867060 | 0.800134 | 1.661685e-48 | -8.615850e-47 |
| Msa0045730 | Msa0867060 | 0.875169 | 3.808238e-68 | -8.615850e-47 |
| Msa0046830 | Msa0867060 | 0.817476 | 3.233700e-52 | -8.615850e-47 |
| Msa0048180 | Msa0867060 | 0.821080 | 4.882119e-53 | -8.615850e-47 |
| Msa0074030 | Msa0867060 | 0.809350 | 1.976535e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867060 | MtrunA17_Chr4g0070981 | 99.516 | 413 | 2 | 0 | 1 | 413 | 1 | 413 | 0.0 | 850 |
| Msa0867060 | MtrunA17_Chr2g0299681 | 93.705 | 413 | 26 | 0 | 1 | 413 | 1 | 413 | 0.0 | 803 |
| Msa0867060 | MtrunA17_Chr8g0342861 | 91.525 | 413 | 35 | 0 | 1 | 413 | 1 | 413 | 0.0 | 785 |
| Msa0867060 | MtrunA17_Chr2g0329931 | 91.525 | 413 | 35 | 0 | 1 | 413 | 1 | 413 | 0.0 | 782 |
| Msa0867060 | MtrunA17_Chr2g0297261 | 64.879 | 373 | 131 | 0 | 41 | 413 | 34 | 406 | 0.0 | 512 |
| Msa0867060 | MtrunA17_Chr1g0169331 | 60.152 | 394 | 120 | 5 | 56 | 413 | 6 | 398 | 5.30e-161 | 458 |
| Msa0867060 | MtrunA17_Chr8g0342851 | 61.272 | 346 | 102 | 4 | 71 | 413 | 3 | 319 | 8.21e-140 | 402 |
| Msa0867060 | MtrunA17_Chr7g0247191 | 39.948 | 388 | 220 | 3 | 19 | 406 | 139 | 513 | 9.25e-91 | 283 |
| Msa0867060 | MtrunA17_Chr7g0247181 | 39.433 | 388 | 222 | 3 | 19 | 406 | 129 | 503 | 1.34e-89 | 280 |
| Msa0867060 | MtrunA17_Chr7g0247221 | 39.433 | 388 | 222 | 3 | 19 | 406 | 132 | 506 | 2.22e-89 | 280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0867060 | AT3G13920.1 | 91.283 | 413 | 35 | 1 | 1 | 413 | 1 | 412 | 0.0 | 776 |
| Msa0867060 | AT1G54270.1 | 91.304 | 414 | 33 | 2 | 1 | 413 | 1 | 412 | 0.0 | 771 |
| Msa0867060 | AT3G13920.4 | 90.073 | 413 | 35 | 2 | 1 | 413 | 1 | 407 | 0.0 | 759 |
| Msa0867060 | AT1G72730.1 | 88.164 | 414 | 48 | 1 | 1 | 413 | 1 | 414 | 0.0 | 756 |
| Msa0867060 | AT1G54270.2 | 90.097 | 414 | 33 | 3 | 1 | 413 | 1 | 407 | 0.0 | 754 |
| Msa0867060 | AT3G13920.3 | 93.766 | 385 | 24 | 0 | 29 | 413 | 18 | 402 | 0.0 | 745 |
| Msa0867060 | AT3G13920.2 | 91.560 | 391 | 32 | 1 | 1 | 391 | 1 | 390 | 0.0 | 736 |
| Msa0867060 | AT3G13920.5 | 91.560 | 391 | 32 | 1 | 1 | 391 | 5 | 394 | 0.0 | 736 |
| Msa0867060 | AT3G19760.1 | 64.191 | 377 | 135 | 0 | 37 | 413 | 32 | 408 | 4.66e-180 | 508 |
| Msa0867060 | AT1G51380.1 | 58.808 | 369 | 152 | 0 | 41 | 409 | 23 | 391 | 7.95e-162 | 461 |
Find 148 sgRNAs with CRISPR-Local
Find 334 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGCAACTTTCTGCTC+TGG | 0.488201 | 5_4:-69719095 | Msa0867060:CDS |
| AAACACGAGAAGACCCAGAA+CGG | 0.652225 | 5_4:+69717626 | None:intergenic |
| AAACTGCTTAATACCCTCTA+AGG | 0.342172 | 5_4:+69717855 | None:intergenic |
| AAAGAGGACACTTGTTAAAA+TGG | 0.225467 | 5_4:-69721500 | Msa0867060:five_prime_UTR |
| AAAGCGACGGAGTTAGTTAA+CGG | 0.531964 | 5_4:+69721229 | None:intergenic |
| AACAGGCTCAGTCTGGAACC+GGG | 0.573707 | 5_4:-69719118 | Msa0867060:CDS |
| AACATCAAGTCCCTTGCAGA+AGG | 0.540831 | 5_4:+69719145 | None:intergenic |
| AACTCCCCGGATCTACGCCG+CGG | 0.720835 | 5_4:+69721269 | None:intergenic |
| AACTCCGCGGCGTAGATCCG+GGG | 0.585487 | 5_4:-69721273 | Msa0867060:five_prime_UTR |
| AAGCAACAGGGTAAGGTAAA+GGG | 0.468637 | 5_4:+69721356 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAGTTGGAAAAGCAAAG+AGG | + | chr5_4:69717448-69717467 | None:intergenic | 30.0% | |
| AAACAACTTATTTGCAGGAA+TGG | + | chr5_4:69717876-69717895 | None:intergenic | 30.0% | |
| AGTGAATGATTGTTACCAAA+CGG | + | chr5_4:69718558-69718577 | None:intergenic | 30.0% | |
| TTGGTTGAATTTGTAGTGTT+TGG | + | chr5_4:69718760-69718779 | None:intergenic | 30.0% | |
| TGAAAGATGCCTTATATTTG+GGG | - | chr5_4:69719083-69719102 | Msa0867060:CDS | 30.0% | |
| TCCTTATAATTAGTCATGGA+AGG | - | chr5_4:69719125-69719144 | Msa0867060:CDS | 30.0% | |
| TCCTTCCATGACTAATTATA+AGG | + | chr5_4:69719129-69719148 | None:intergenic | 30.0% | |
| CAAACATCTTGATGTAATCT+GGG | + | chr5_4:69719652-69719671 | None:intergenic | 30.0% | |
| ACAAACATCTTGATGTAATC+TGG | + | chr5_4:69719653-69719672 | None:intergenic | 30.0% | |
| TTACATCAAGATGTTTGTGT+TGG | - | chr5_4:69719655-69719674 | Msa0867060:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 69716966 | 69721531 | 69716966 | ID=Msa0867060;Name=Msa0867060 |
| chr5_4 | mRNA | 69716966 | 69721531 | 69716966 | ID=Msa0867060-mRNA-1;Parent=Msa0867060;Name=Msa0867060-mRNA-1;_AED=0.12;_eAED=0.12;_QI=326|1|1|1|0.75|0.6|5|415|413 |
| chr5_4 | exon | 69716966 | 69718016 | 69716966 | ID=Msa0867060-mRNA-1:exon:21887;Parent=Msa0867060-mRNA-1 |
| chr5_4 | exon | 69718779 | 69719209 | 69718779 | ID=Msa0867060-mRNA-1:exon:21886;Parent=Msa0867060-mRNA-1 |
| chr5_4 | exon | 69720242 | 69720348 | 69720242 | ID=Msa0867060-mRNA-1:exon:21885;Parent=Msa0867060-mRNA-1 |
| chr5_4 | exon | 69720463 | 69720532 | 69720463 | ID=Msa0867060-mRNA-1:exon:21884;Parent=Msa0867060-mRNA-1 |
| chr5_4 | exon | 69721208 | 69721531 | 69721208 | ID=Msa0867060-mRNA-1:exon:21883;Parent=Msa0867060-mRNA-1 |
| chr5_4 | five_prime_UTR | 69721208 | 69721531 | 69721208 | ID=Msa0867060-mRNA-1:five_prime_utr;Parent=Msa0867060-mRNA-1 |
| chr5_4 | five_prime_UTR | 69720531 | 69720532 | 69720531 | ID=Msa0867060-mRNA-1:five_prime_utr;Parent=Msa0867060-mRNA-1 |
| chr5_4 | CDS | 69720463 | 69720530 | 69720463 | ID=Msa0867060-mRNA-1:cds;Parent=Msa0867060-mRNA-1 |
| chr5_4 | CDS | 69720242 | 69720348 | 69720242 | ID=Msa0867060-mRNA-1:cds;Parent=Msa0867060-mRNA-1 |
| chr5_4 | CDS | 69718779 | 69719209 | 69718779 | ID=Msa0867060-mRNA-1:cds;Parent=Msa0867060-mRNA-1 |
| chr5_4 | CDS | 69717381 | 69718016 | 69717381 | ID=Msa0867060-mRNA-1:cds;Parent=Msa0867060-mRNA-1 |
| chr5_4 | three_prime_UTR | 69716966 | 69717380 | 69716966 | ID=Msa0867060-mRNA-1:three_prime_utr;Parent=Msa0867060-mRNA-1 |
| Gene Sequence |
>Msa0867060 ATGGCTGGACTTGCACCCGAGGGGTCACAATTCGATGCTGGGAAATTTGATCAAAAAATGAATGACTTGATTGCAGCTGATGGAGATGAATTCTGGACTTCTTATGATGAGGTTTATGATAGTTTTGATGCTATGGGTTTGTTAGAGAATCTCCTCAGGGGCATTTATGCATATGGTTTTGAAAAGCCATCTGCTATTCAGCAAAGGGGGATAGTTCCCTTCTGCAAGGGACTTGATGTTATTCAACAGGCTCAGTCTGGAACCGGGAAAACAGCAACTTTCTGCTCTGGTGTTCTCCAGCAACTTGACTACAATGTGACTCAGTGCCAGGCCTTGGTTTTAGCTCCAACTCGTGAGCTTGCTCAGCAGATTGAGAAGGTTATGCGTGCGCTTGGAGACTATCTAGGTGTTAAGGTGCATGCTTGTGTCGGAGGTACAAGTGTTCGTGAAGACCAACGTATTCTATCAAGTGGTGTTCATGTTGTGGTTGGTACTCCTGGTCGTGTGTTTGATATGCTGCGCAGACAGTCTCTTCACCCAGATTACATCAAGATGTTTGTGTTGGATGAGGCTGATGAAATGCTTTCTCGAGGTTTCAAAGATCAGATCTATGATATCTTTCAGCTGCTGCCAGGTAAGATTCAAGTAGGAGTTTTCTCTGCTACAATGCCTCCTGAGGCACTTGAGATCACAAGGAAGTTTATGAACAAACCTGTGAGGATCCTTGTGAAGCGTGATGAGCTTACCTTAGAGGGTATTAAGCAGTTTTATGTCAATGTTGAGAAAGAGGAGTGGAAGCTGGACACACTCTGTGATCTTTATGAGACATTAGCCATCACTCAGAGTGTCATTTTTGTGAACACCAGGAGGAAAGTTGATTGGTTGACTGACAAAATGCGAAGCCGAGACCATACAGTCTCAGCTACTCACGGAGACATGGATCAAAATACCAGGGACATTATTATGCGTGAATTCCGTTCTGGGTCTTCTCGTGTTTTAATAACTACCGATCTGTTGGCTCGTGGTATTGATGTCCAACAAGTGTCTCTGGTTATAAATTTTGACCTCCCTACCCAGCCTGAGAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGTAGAAAAGGTGTTGCCATTAACTTTGTCACGAAGGAAGATGAAGGAATGCTACGAGATATCCAGAAGTTTTACAATGTGTTAGTTGAGGAGCTGCCATCAAATGTTGCTGAGCTTCTCTGA |
| Protein sequence |
>Msa0867060 MAGLAPEGSQFDAGKFDQKMNDLIAADGDEFWTSYDEVYDSFDAMGLLENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGVLQQLDYNVTQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILSSGVHVVVGTPGRVFDMLRRQSLHPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPGKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVEKEEWKLDTLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINFDLPTQPENYLHRIGRSGRFGRKGVAINFVTKEDEGMLRDIQKFYNVLVEELPSNVAELL* |
