AlfalfaGEDB | Msa1298620

M. sativa cultivar zhongmu-4 / Msa1298620

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564
PFAM: PF13365.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1298620	sp\|Q9FL12\|DEGP9_ARATH	83.721	387	63	0	10	396	202	588	0.0	668

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1298620	G7JNU4	99.483	387	2	0	10	396	204	590	0.0	791

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0046830	Msa1298620	0.803087	4.121537e-49	-8.615850e-47
Msa0188690	Msa1298620	0.842442	2.580198e-58	-8.615850e-47
Msa0270210	Msa1298620	0.860575	1.877808e-63	-8.615850e-47
Msa0307850	Msa1298620	0.825556	4.395255e-54	-8.615850e-47
Msa0706090	Msa1298620	0.802929	4.443600e-49	-8.615850e-47
Msa0790400	Msa1298620	0.838197	3.318718e-57	-8.615850e-47
Msa1120790	Msa1298620	0.842006	3.364105e-58	-8.615850e-47
Msa1123350	Msa1298620	0.810621	1.052497e-50	-8.615850e-47
Msa1171960	Msa1298620	0.843211	1.610523e-58	-8.615850e-47
Msa1298620	Msa1356610	0.802750	4.838056e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1298620	MtrunA17_Chr4g0059321	99.483	387	2	0	10	396	204	590	0.0	791
Msa1298620	MtrunA17_Chr1g0196111	50.909	385	184	2	10	394	51	430	1.86e-137	400
Msa1298620	MtrunA17_Chr6g0448981	51.169	385	184	3	10	394	157	537	3.65e-132	390

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1298620	AT5G40200.1	83.721	387	63	0	10	396	202	588	0.0	668
Msa1298620	AT2G47940.2	50.909	385	184	2	10	394	191	570	1.89e-135	400
Msa1298620	AT2G47940.1	50.909	385	184	2	10	394	192	571	1.93e-135	400
Msa1298620	AT5G36950.1	50.000	386	188	4	10	394	189	570	3.16e-124	371
Msa1298620	AT1G65630.1	44.041	386	202	3	10	395	171	542	9.18e-103	315
Msa1298620	AT1G65640.2	43.523	386	199	4	10	395	149	515	2.59e-99	305
Msa1298620	AT5G40560.1	42.078	385	195	6	10	394	116	472	2.56e-87	273
Msa1298620	AT3G16540.1	37.922	385	199	4	10	394	186	530	2.09e-81	260
Msa1298620	AT1G65640.1	43.709	302	156	3	10	311	149	436	7.48e-79	249
Msa1298620	AT3G16550.1	37.240	384	211	4	10	393	146	499	1.25e-75	243

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Find 135 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCATGATAACTGGCGT+TGG	0.591084	8_3:+59452731	None:intergenic
AAATTCAATATTAAACTTGA+TGG	0.313467	8_3:-59451578	Msa1298620:CDS
AACACTGTAACCTGTGTAGC+AGG	0.499323	8_3:+59453571	None:intergenic
AACAGTTACTGCATCTTGAA+GGG	0.570653	8_3:+59453074	None:intergenic
AAGCAAGCCTTTGTTCATTT+TGG	0.219210	8_3:-59453460	Msa1298620:five_prime_UTR
AAGCTAAGGATAACATCTGA+CGG	0.633148	8_3:+59452412	None:intergenic
AATACGTGATACAACGCCAC+TGG	0.620175	8_3:+59453014	None:intergenic
AATGGCCAGCAATCAGTGGT+GGG	0.634773	8_3:+59453418	None:intergenic
AATGGTAAAGGAACCTGTGT+GGG	0.518960	8_3:-59452809	Msa1298620:CDS
ACAACTTGTTGTAATTTCTC+AGG	0.458392	8_3:-59451102	Msa1298620:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATTGATGAAGGAAAACC+AGG	+	chr8_3:59451304-59451323	None:intergenic	30.0%
GCAAGAAACAAAATTGATGA+AGG	+	chr8_3:59451313-59451332	None:intergenic	30.0%
ATAGATGCTGCTATAAATTC+TGG	-	chr8_3:59451335-59451354	Msa1298620:intron	30.0%
GTTATCATTGTGTCTAAAAG+AGG	+	chr8_3:59451983-59452002	None:intergenic	30.0%
ATGTTAAAGATGGACAGATA+TGG	+	chr8_3:59452188-59452207	None:intergenic	30.0%
TTAGTACCATGTTATGGTTT+AGG	-	chr8_3:59452220-59452239	Msa1298620:intron	30.0%
AACAAACCTAAACCATAACA+TGG	+	chr8_3:59452229-59452248	None:intergenic	30.0%
TAAAAAGCAGAGAACAACAT+AGG	+	chr8_3:59452377-59452396	None:intergenic	30.0%
CTCATATCACAGAAATATAC+TGG	-	chr8_3:59452545-59452564	Msa1298620:CDS	30.0%
CCTGCAATGATATAATATGA+GGG	+	chr8_3:59452677-59452696	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	59450579	59453632	59450579	ID=Msa1298620;Name=Msa1298620
chr8_3	mRNA	59450579	59453632	59450579	ID=Msa1298620-mRNA-1;Parent=Msa1298620;Name=Msa1298620-mRNA-1;_AED=0.10;_eAED=0.10;_QI=439\|0.71\|0.62\|1\|1\|0.87\|8\|0\|396
chr8_3	exon	59450579	59450674	59450579	ID=Msa1298620-mRNA-1:exon:17165;Parent=Msa1298620-mRNA-1
chr8_3	exon	59450756	59450860	59450756	ID=Msa1298620-mRNA-1:exon:17164;Parent=Msa1298620-mRNA-1
chr8_3	exon	59450968	59451018	59450968	ID=Msa1298620-mRNA-1:exon:17163;Parent=Msa1298620-mRNA-1
chr8_3	exon	59451103	59451204	59451103	ID=Msa1298620-mRNA-1:exon:17162;Parent=Msa1298620-mRNA-1
chr8_3	exon	59451472	59451704	59451472	ID=Msa1298620-mRNA-1:exon:17161;Parent=Msa1298620-mRNA-1
chr8_3	exon	59452380	59452571	59452380	ID=Msa1298620-mRNA-1:exon:17160;Parent=Msa1298620-mRNA-1
chr8_3	exon	59452696	59452876	59452696	ID=Msa1298620-mRNA-1:exon:17159;Parent=Msa1298620-mRNA-1
chr8_3	exon	59452966	59453632	59452966	ID=Msa1298620-mRNA-1:exon:17158;Parent=Msa1298620-mRNA-1
chr8_3	five_prime_UTR	59453194	59453632	59453194	ID=Msa1298620-mRNA-1:five_prime_utr;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59452966	59453193	59452966	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59452696	59452876	59452696	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59452380	59452571	59452380	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59451472	59451704	59451472	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59451103	59451204	59451103	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59450968	59451018	59450968	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59450756	59450860	59450756	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1
chr8_3	CDS	59450579	59450674	59450579	ID=Msa1298620-mRNA-1:cds;Parent=Msa1298620-mRNA-1

Gene Sequence

>Msa1298620

ATGTGTGTGTTTTATTTTACATTTGCAGCCATGCTTACTGTTGACGATGATGAGTTCTGGCAAGGGATGTCGCCTGTGGATTTTGGGGAGTTGCCTACCCTTCAAGATGCAGTAACTGTTGTGGGCTACCCAATTGGTGGAGACACTATCTCTGTGACCAGTGGCGTTGTATCACGTATTGAGATTTTATCTTATGTCCATGGTTCCACAGAGCTTCTAGGTTTACAGATAGATGCTGCTATAAATTCTGGAAATTCTGGTGGACCTGCATTTAATGGTAAAGGAACCTGTGTGGGGATAGCATTTCAGTCCCTCAAACATGAAGATGTGGAGAATATAGGTTATGTCATTCCAACGCCAGTTATCATGCATTTCATCCAGGATTATGAGAAGAATGGAGGATATACTGGATTCCCTATTCTCGGGGTTGAGTGGCAGAAAATGGAAAATCCTGATCTACGAATGGCAATGGGCATGAAATCTGATCAGAAAGGTGTGCGCATTAGAAGAATCGATCCTACTTCTCCTGAATCTCAGGTTTTGAAACCGTCAGATGTTATCCTTAGCTTTGATGGGGTTGATATTGCCAATGATGGAACAGTTCCATTTCGCCATGGAGAGCGCATTGGTTTCAGCTATCTCATATCACAGAAATATACTGGCGATGATGCAGCAATCAAAGTATTGCGGAATTCAAATGTTCTCAAATTCAATATTAAACTTGATGGTCATAGAAGGCTTATTCCAGCACACAGCAAGGGCAAGCCTCCCTCATATTATATCATTGCAGGATTTGTTTTTTCAACGGTTTCGGTTCCTTATCTTCGTTCTGAGTACGGGAAGGACTATGAATATGAAGCTCCAGTCAAGATTTTGGATAAACTGCTGTATGCAATGCCACAATCACCAGATGAACAACTTGTTGTAATTTCTCAGGTGCTGGTGGCTGATATTAACATCGGATATGAAGAGATCGTTAACACCCAGGTCGTTGGTTTTAATGGTAAACCTGTAAAAAATCTTAAGAGCTTGGCCGCAATGGTAGAGAGCTGTGATGATGAGTATCTGAAATTTGATTTAGATTATGAGCAGATAGTGGTCCTTCGCACGAAGACTGCAAAAGCTGCCACGCTAGATATTCTTGCAACACACTGCATTCCATCAGCAATGTCTGACGATCTCAAGTCA

Protein sequence

>Msa1298620

MCVFYFTFAAMLTVDDDEFWQGMSPVDFGELPTLQDAVTVVGYPIGGDTISVTSGVVSRIEILSYVHGSTELLGLQIDAAINSGNSGGPAFNGKGTCVGIAFQSLKHEDVENIGYVIPTPVIMHFIQDYEKNGGYTGFPILGVEWQKMENPDLRMAMGMKSDQKGVRIRRIDPTSPESQVLKPSDVILSFDGVDIANDGTVPFRHGERIGFSYLISQKYTGDDAAIKVLRNSNVLKFNIKLDGHRRLIPAHSKGKPPSYYIIAGFVFSTVSVPYLRSEYGKDYEYEAPVKILDKLLYAMPQSPDEQLVVISQVLVADINIGYEEIVNTQVVGFNGKPVKNLKSLAAMVESCDDEYLKFDLDYEQIVVLRTKTAKAATLDILATHCIPSAMSDDLKS