AlfalfaGEDB | Msa1174580

M. sativa cultivar zhongmu-4 / Msa1174580

KEGG: -
GO: -
PFAM: PF07911.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174580	XP_003627329.2	99.359	156	1	0	1	156	1	156	8.30e-109	316

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174580	G7L7L9	99.359	156	1	0	1	156	1	156	3.97e-109	316

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0178510	Msa1174580	0.802234	6.179525e-49	-8.615850e-47
Msa0191890	Msa1174580	0.835189	1.939763e-56	-8.615850e-47
Msa0312530	Msa1174580	0.825926	3.590405e-54	-8.615850e-47
Msa1080960	Msa1174580	0.809879	1.521444e-50	-8.615850e-47
Msa1174580	Msa1219900	0.805011	1.640130e-49	-8.615850e-47
Msa1174580	Msa1266490	0.819940	8.917277e-53	-8.615850e-47
Msa1174580	Msa1296890	0.813976	1.948889e-51	-8.615850e-47
Msa1174580	Msa1307550	0.815375	9.549113e-52	-8.615850e-47
Msa1174580	Msa1315350	0.808484	3.027174e-50	-8.615850e-47
Msa1174580	Msa1319820	0.882541	9.553384e-71	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174580	MtrunA17_Chr8g0344381	99.359	156	1	0	1	156	1	156	7.64e-113	316
Msa1174580	MtrunA17_Chr4g0070351	53.390	118	54	1	6	122	5	122	1.85e-37	124
Msa1174580	MtrunA17_Chr3g0136441	51.613	124	49	4	30	152	11	124	2.88e-37	124
Msa1174580	MtrunA17_Chr4g0070371	52.991	117	51	3	10	122	1	117	7.44e-35	119
Msa1174580	MtrunA17_Chr8g0340421	46.970	132	56	5	29	154	61	184	3.41e-32	113
Msa1174580	MtrunA17_Chr5g0394601	50.000	104	44	2	30	133	2	97	1.88e-31	108
Msa1174580	MtrunA17_Chr1g0150711	45.600	125	55	5	34	153	20	136	1.65e-29	105
Msa1174580	MtrunA17_Chr2g0331111	42.969	128	69	3	29	154	61	186	4.45e-26	97.8
Msa1174580	MtrunA17_Chr1g0205251	45.556	90	43	2	30	118	2	86	5.74e-26	95.5
Msa1174580	MtrunA17_Chr3g0140381	43.519	108	59	2	30	136	36	142	2.30e-25	94.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1174580	AT1G72510.1	53.939	165	65	4	1	154	1	165	8.88e-57	175
Msa1174580	AT1G72510.2	53.939	165	65	4	1	154	1	165	8.88e-57	175
Msa1174580	AT2G09970.1	48.193	166	74	5	1	154	1	166	4.34e-47	150
Msa1174580	AT5G20670.1	44.079	152	64	5	16	155	11	153	4.09e-31	109
Msa1174580	AT3G22540.1	44.860	107	54	2	30	136	2	103	1.96e-28	101
Msa1174580	AT4G14819.1	50.495	101	40	3	30	130	2	92	2.34e-28	101
Msa1174580	AT1G54095.1	41.727	139	67	4	29	156	14	149	7.66e-28	101
Msa1174580	AT5G25840.1	41.135	141	70	5	19	154	31	163	2.98e-24	92.8
Msa1174580	AT1G79770.1	44.828	116	58	3	6	121	31	140	2.20e-23	90.5
Msa1174580	AT2G25780.1	36.207	116	63	4	36	149	41	147	1.19e-18	77.8

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Find 26 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAACTGCTGGATTTGG+AGG	0.460663	8_1:+5675734	None:intergenic
AAATGGGTTTGTGGATTATG+TGG	0.572200	8_1:-5675863	Msa1174580:CDS
AAGACTACTAGAGAAATTCA+TGG	0.460424	8_1:-5675644	Msa1174580:CDS
ACAGATTCTTAGGAGGAGTT+TGG	0.368015	8_1:-5675696	Msa1174580:CDS
ACATAGAAAGAATCAGAGAA+AGG	0.563326	8_1:-5675895	Msa1174580:CDS
ACTAGAGAAATTCATGGTTC+TGG	0.385021	8_1:-5675638	Msa1174580:CDS
AGAAAGGTATCAAGGAAAAT+GGG	0.478410	8_1:-5675879	Msa1174580:CDS
AGAATCAGAGAAAGGTATCA+AGG	0.494211	8_1:-5675887	Msa1174580:CDS
AGCTGCTTCTCTACTCTTTC+TGG	0.087534	8_1:-5675599	Msa1174580:CDS
ATCAAATGAACTGCTGGATT+TGG	0.317985	8_1:+5675731	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATAGAAAGAATCAGAGAA+AGG	-	chr8_1:5675737-5675756	Msa1174580:CDS	30.0%
AAGACTACTAGAGAAATTCA+TGG	-	chr8_1:5675988-5676007	Msa1174580:CDS	30.0%
TTTGCAATATGTGAATGCTG+TGG	-	chr8_1:5675688-5675707	Msa1174580:CDS	35.0%
CTGATTCTTTCTATGTATGC+TGG	+	chr8_1:5675733-5675752	None:intergenic	35.0%
ATCAAATGAACTGCTGGATT+TGG	+	chr8_1:5675904-5675923	None:intergenic	35.0%
ACTAGAGAAATTCATGGTTC+TGG	-	chr8_1:5675994-5676013	Msa1174580:CDS	35.0%
AAATGAACTGCTGGATTTGG+AGG	+	chr8_1:5675901-5675920	None:intergenic	40.0%
GCTGAAATCAAATGAACTGC+TGG	+	chr8_1:5675910-5675929	None:intergenic	40.0%
CAGCTATGAGACAGATTCTT+AGG	-	chr8_1:5675926-5675945	Msa1174580:CDS	40.0%
CTATGAGACAGATTCTTAGG+AGG	-	chr8_1:5675929-5675948	Msa1174580:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	5675592	5676062	5675592	ID=Msa1174580;Name=Msa1174580
chr8_1	mRNA	5675592	5676062	5675592	ID=Msa1174580-mRNA-1;Parent=Msa1174580;Name=Msa1174580-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|156
chr8_1	exon	5675592	5676062	5675592	ID=Msa1174580-mRNA-1:exon:1885;Parent=Msa1174580-mRNA-1
chr8_1	CDS	5675592	5676062	5675592	ID=Msa1174580-mRNA-1:cds;Parent=Msa1174580-mRNA-1

Gene Sequence

>Msa1174580

ATGTCTGCAACAATGATAAGTGATCCAATGATTATATCAACACAAGAAACTCAATCAACAACTAGTAGCATACTAGTTGCACAGAGTGAAGTTGAATTTGCAATATGTGAATGCTGTGGACTAACAGAAGAGTGCACACCAGCATACATAGAAAGAATCAGAGAAAGGTATCAAGGAAAATGGGTTTGTGGATTATGTGGTGAAGCTGTTAAAGATGAAATTGTAAGGTCAGAAAGACTTGTAAGCACAGAAGAAGCAATGACAAAACATATGAACTTTTGCAAAAAATTCAATACTTCAGGTCCTCCTCCAAATCCAGCAGTTCATTTGATTTCAGCTATGAGACAGATTCTTAGGAGGAGTTTGGTTAGGTCCACACCAAGTAGTCCAATTAATAAGACTACTAGAGAAATTCATGGTTCTGGACTTGCTAGGTCTGAAAGCTGCTTCTCTACTCTTTCTGGTTCTTGA

Protein sequence

>Msa1174580

MSATMISDPMIISTQETQSTTSSILVAQSEVEFAICECCGLTEECTPAYIERIRERYQGKWVCGLCGEAVKDEIVRSERLVSTEEAMTKHMNFCKKFNTSGPPPNPAVHLISAMRQILRRSLVRSTPSSPINKTTREIHGSGLARSESCFSTLSGS*