Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174580 | XP_003627329.2 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 8.30e-109 | 316 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174580 | G7L7L9 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 3.97e-109 | 316 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0178510 | Msa1174580 | 0.802234 | 6.179525e-49 | -8.615850e-47 |
| Msa0191890 | Msa1174580 | 0.835189 | 1.939763e-56 | -8.615850e-47 |
| Msa1080960 | Msa1174580 | 0.809879 | 1.521444e-50 | -8.615850e-47 |
| Msa0312530 | Msa1174580 | 0.825926 | 3.590405e-54 | -8.615850e-47 |
| Msa1174580 | Msa1219900 | 0.805011 | 1.640130e-49 | -8.615850e-47 |
| Msa1174580 | Msa1266490 | 0.819940 | 8.917277e-53 | -8.615850e-47 |
| Msa1174580 | Msa1296890 | 0.813976 | 1.948889e-51 | -8.615850e-47 |
| Msa1174580 | Msa1307550 | 0.815375 | 9.549113e-52 | -8.615850e-47 |
| Msa1174580 | Msa1315350 | 0.808484 | 3.027174e-50 | -8.615850e-47 |
| Msa1174580 | Msa1319820 | 0.882541 | 9.553384e-71 | -8.615850e-47 |
| Msa1174580 | Msa1349450 | 0.801338 | 9.439726e-49 | -8.615850e-47 |
| Msa1174580 | Msa1401270 | 0.834813 | 2.411802e-56 | -8.615850e-47 |
| Msa1174580 | Msa1409490 | 0.806067 | 9.846713e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174580 | MtrunA17_Chr8g0344381 | 99.359 | 156 | 1 | 0 | 1 | 156 | 1 | 156 | 7.64e-113 | 316 |
| Msa1174580 | MtrunA17_Chr4g0070351 | 53.390 | 118 | 54 | 1 | 6 | 122 | 5 | 122 | 1.85e-37 | 124 |
| Msa1174580 | MtrunA17_Chr3g0136441 | 51.613 | 124 | 49 | 4 | 30 | 152 | 11 | 124 | 2.88e-37 | 124 |
| Msa1174580 | MtrunA17_Chr4g0070371 | 52.991 | 117 | 51 | 3 | 10 | 122 | 1 | 117 | 7.44e-35 | 119 |
| Msa1174580 | MtrunA17_Chr8g0340421 | 46.970 | 132 | 56 | 5 | 29 | 154 | 61 | 184 | 3.41e-32 | 113 |
| Msa1174580 | MtrunA17_Chr5g0394601 | 50.000 | 104 | 44 | 2 | 30 | 133 | 2 | 97 | 1.88e-31 | 108 |
| Msa1174580 | MtrunA17_Chr1g0150711 | 45.600 | 125 | 55 | 5 | 34 | 153 | 20 | 136 | 1.65e-29 | 105 |
| Msa1174580 | MtrunA17_Chr2g0331111 | 42.969 | 128 | 69 | 3 | 29 | 154 | 61 | 186 | 4.45e-26 | 97.8 |
| Msa1174580 | MtrunA17_Chr1g0205251 | 45.556 | 90 | 43 | 2 | 30 | 118 | 2 | 86 | 5.74e-26 | 95.5 |
| Msa1174580 | MtrunA17_Chr3g0140381 | 43.519 | 108 | 59 | 2 | 30 | 136 | 36 | 142 | 2.30e-25 | 94.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174580 | AT1G72510.1 | 53.939 | 165 | 65 | 4 | 1 | 154 | 1 | 165 | 8.88e-57 | 175 |
| Msa1174580 | AT1G72510.2 | 53.939 | 165 | 65 | 4 | 1 | 154 | 1 | 165 | 8.88e-57 | 175 |
| Msa1174580 | AT2G09970.1 | 48.193 | 166 | 74 | 5 | 1 | 154 | 1 | 166 | 4.34e-47 | 150 |
| Msa1174580 | AT5G20670.1 | 44.079 | 152 | 64 | 5 | 16 | 155 | 11 | 153 | 4.09e-31 | 109 |
| Msa1174580 | AT3G22540.1 | 44.860 | 107 | 54 | 2 | 30 | 136 | 2 | 103 | 1.96e-28 | 101 |
| Msa1174580 | AT4G14819.1 | 50.495 | 101 | 40 | 3 | 30 | 130 | 2 | 92 | 2.34e-28 | 101 |
| Msa1174580 | AT1G54095.1 | 41.727 | 139 | 67 | 4 | 29 | 156 | 14 | 149 | 7.66e-28 | 101 |
| Msa1174580 | AT5G25840.1 | 41.135 | 141 | 70 | 5 | 19 | 154 | 31 | 163 | 2.98e-24 | 92.8 |
| Msa1174580 | AT1G79770.1 | 44.828 | 116 | 58 | 3 | 6 | 121 | 31 | 140 | 2.20e-23 | 90.5 |
| Msa1174580 | AT2G25780.1 | 36.207 | 116 | 63 | 4 | 36 | 149 | 41 | 147 | 1.19e-18 | 77.8 |
Find 26 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTGCTTCTCTACTCTTTC+TGG | 0.087534 | 8_1:-5675599 | Msa1174580:CDS |
| GAGAAAGGTATCAAGGAAAA+TGG | 0.211571 | 8_1:-5675880 | Msa1174580:CDS |
| TCTCTAGTAGTCTTATTAAT+TGG | 0.222630 | 8_1:+5675653 | None:intergenic |
| CTACTCTTTCTGGTTCTTGA+TGG | 0.251674 | 8_1:-5675589 | None:intergenic |
| CAGCTATGAGACAGATTCTT+AGG | 0.252337 | 8_1:-5675706 | Msa1174580:CDS |
| ATCAAATGAACTGCTGGATT+TGG | 0.317985 | 8_1:+5675731 | None:intergenic |
| TCTTGTGTTGATATAATCAT+TGG | 0.343229 | 8_1:+5676016 | None:intergenic |
| ACAGATTCTTAGGAGGAGTT+TGG | 0.368015 | 8_1:-5675696 | Msa1174580:CDS |
| CTGATTCTTTCTATGTATGC+TGG | 0.380458 | 8_1:+5675902 | None:intergenic |
| ACTAGAGAAATTCATGGTTC+TGG | 0.385021 | 8_1:-5675638 | Msa1174580:CDS |
| GTCTTATTAATTGGACTACT+TGG | 0.397180 | 8_1:+5675662 | None:intergenic |
| GCTGAAATCAAATGAACTGC+TGG | 0.441735 | 8_1:+5675725 | None:intergenic |
| AAGACTACTAGAGAAATTCA+TGG | 0.460424 | 8_1:-5675644 | Msa1174580:CDS |
| AAATGAACTGCTGGATTTGG+AGG | 0.460663 | 8_1:+5675734 | None:intergenic |
| TTCTTAGGAGGAGTTTGGTT+AGG | 0.460993 | 8_1:-5675691 | Msa1174580:CDS |
| TATCAAGGAAAATGGGTTTG+TGG | 0.478219 | 8_1:-5675872 | Msa1174580:CDS |
| AGAAAGGTATCAAGGAAAAT+GGG | 0.478410 | 8_1:-5675879 | Msa1174580:CDS |
| AGAATCAGAGAAAGGTATCA+AGG | 0.494211 | 8_1:-5675887 | Msa1174580:CDS |
| CTGTTAAAGATGAAATTGTA+AGG | 0.538879 | 8_1:-5675835 | Msa1174580:CDS |
| ATTAATTGGACTACTTGGTG+TGG | 0.561690 | 8_1:+5675667 | None:intergenic |
| ACATAGAAAGAATCAGAGAA+AGG | 0.563326 | 8_1:-5675895 | Msa1174580:CDS |
| AAATGGGTTTGTGGATTATG+TGG | 0.572200 | 8_1:-5675863 | Msa1174580:CDS |
| TGAACTGCTGGATTTGGAGG+AGG | 0.575747 | 8_1:+5675737 | None:intergenic |
| CTATGAGACAGATTCTTAGG+AGG | 0.602915 | 8_1:-5675703 | Msa1174580:CDS |
| TTCATGGTTCTGGACTTGCT+AGG | 0.647756 | 8_1:-5675628 | Msa1174580:CDS |
| TTTGCAATATGTGAATGCTG+TGG | 0.702632 | 8_1:-5675944 | Msa1174580:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTTGTGTTGATATAATCAT+TGG | + | chr8_1:5675619-5675638 | None:intergenic | 25.0% |
| ! | CTGTTAAAGATGAAATTGTA+AGG | - | chr8_1:5675797-5675816 | Msa1174580:CDS | 25.0% |
| ! | TGCAAAAAATTCAATACTTC+AGG | - | chr8_1:5675871-5675890 | Msa1174580:CDS | 25.0% |
| !!! | TCTCTAGTAGTCTTATTAAT+TGG | + | chr8_1:5675982-5676001 | None:intergenic | 25.0% |
| ACATAGAAAGAATCAGAGAA+AGG | - | chr8_1:5675737-5675756 | Msa1174580:CDS | 30.0% | |
| !! | AGAAAGGTATCAAGGAAAAT+GGG | - | chr8_1:5675753-5675772 | Msa1174580:CDS | 30.0% |
| ! | GTCTTATTAATTGGACTACT+TGG | + | chr8_1:5675973-5675992 | None:intergenic | 30.0% |
| AAGACTACTAGAGAAATTCA+TGG | - | chr8_1:5675988-5676007 | Msa1174580:CDS | 30.0% | |
| TTTGCAATATGTGAATGCTG+TGG | - | chr8_1:5675688-5675707 | Msa1174580:CDS | 35.0% | |
| CTGATTCTTTCTATGTATGC+TGG | + | chr8_1:5675733-5675752 | None:intergenic | 35.0% | |
| !! | AGAATCAGAGAAAGGTATCA+AGG | - | chr8_1:5675745-5675764 | Msa1174580:CDS | 35.0% |
| !! | GAGAAAGGTATCAAGGAAAA+TGG | - | chr8_1:5675752-5675771 | Msa1174580:CDS | 35.0% |
| ! | TATCAAGGAAAATGGGTTTG+TGG | - | chr8_1:5675760-5675779 | Msa1174580:CDS | 35.0% |
| ! | AAATGGGTTTGTGGATTATG+TGG | - | chr8_1:5675769-5675788 | Msa1174580:CDS | 35.0% |
| ATCAAATGAACTGCTGGATT+TGG | + | chr8_1:5675904-5675923 | None:intergenic | 35.0% | |
| ! | ATTAATTGGACTACTTGGTG+TGG | + | chr8_1:5675968-5675987 | None:intergenic | 35.0% |
| ACTAGAGAAATTCATGGTTC+TGG | - | chr8_1:5675994-5676013 | Msa1174580:CDS | 35.0% | |
| AAATGAACTGCTGGATTTGG+AGG | + | chr8_1:5675901-5675920 | None:intergenic | 40.0% | |
| GCTGAAATCAAATGAACTGC+TGG | + | chr8_1:5675910-5675929 | None:intergenic | 40.0% | |
| CAGCTATGAGACAGATTCTT+AGG | - | chr8_1:5675926-5675945 | Msa1174580:CDS | 40.0% | |
| CTATGAGACAGATTCTTAGG+AGG | - | chr8_1:5675929-5675948 | Msa1174580:CDS | 40.0% | |
| ACAGATTCTTAGGAGGAGTT+TGG | - | chr8_1:5675936-5675955 | Msa1174580:CDS | 40.0% | |
| TTCTTAGGAGGAGTTTGGTT+AGG | - | chr8_1:5675941-5675960 | Msa1174580:CDS | 40.0% | |
| !! | TTCATGGTTCTGGACTTGCT+AGG | - | chr8_1:5676004-5676023 | Msa1174580:CDS | 45.0% |
| AGCTGCTTCTCTACTCTTTC+TGG | - | chr8_1:5676033-5676052 | Msa1174580:CDS | 45.0% | |
| TGAACTGCTGGATTTGGAGG+AGG | + | chr8_1:5675898-5675917 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 5675592 | 5676062 | 5675592 | ID=Msa1174580;Name=Msa1174580 |
| chr8_1 | mRNA | 5675592 | 5676062 | 5675592 | ID=Msa1174580-mRNA-1;Parent=Msa1174580;Name=Msa1174580-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|156 |
| chr8_1 | exon | 5675592 | 5676062 | 5675592 | ID=Msa1174580-mRNA-1:exon:1885;Parent=Msa1174580-mRNA-1 |
| chr8_1 | CDS | 5675592 | 5676062 | 5675592 | ID=Msa1174580-mRNA-1:cds;Parent=Msa1174580-mRNA-1 |
| Gene Sequence |
| Protein sequence |
