AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1182910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1182910 MtrunA17_Chr4g0042641 85.714 28 4 0 32 59 17 44 1.16e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATATCTATATCAGCTTT+TGG 0.255888 8_1:-18065266 Msa1182910:three_prime_UTR
TGATTTCATGGATGGAGGTT+GGG 0.325455 8_1:+18067591 Msa1182900:intron
GCTGATGAATTTCAAGAAAT+AGG 0.329504 8_1:-18067057 Msa1182910:intron
GAGATAACAACACACACTTC+TGG 0.338725 8_1:-18067239 Msa1182910:five_prime_UTR
ACACAAGCAGTGCAAATAAC+AGG 0.339261 8_1:+18065351 Msa1182900:intron
ACTCTCCAAGACCTCATCTT+TGG 0.363030 8_1:+18065300 Msa1182900:intron
GTAGAAGAAAGATGATTTCA+TGG 0.387224 8_1:+18067579 Msa1182900:intron
GATTTCATGGATGGAGGTTG+GGG 0.404674 8_1:+18067592 Msa1182900:intron
GATTAATTAAAATAAAATAA+AGG 0.418164 8_1:+18065232 Msa1182900:intron
ATGATTTCATGGATGGAGGT+TGG 0.434492 8_1:+18067590 Msa1182900:intron
GTGTGGTTGGAAAGCGGCAC+AGG 0.438612 8_1:-18065332 Msa1182910:CDS
CTATTCCAAAGATGAGGTCT+TGG 0.445964 8_1:-18065305 Msa1182910:exon
AGATAACAACACACACTTCT+GGG 0.464005 8_1:-18067238 Msa1182910:five_prime_UTR
ATTTGCACTGCTTGTGTGGT+TGG 0.468289 8_1:-18065345 Msa1182910:CDS
AGGGACGATATGAGGATTAC+CGG 0.476410 8_1:-18065388 Msa1182910:CDS
TGTTATTTGCACTGCTTGTG+TGG 0.507323 8_1:-18065349 Msa1182910:CDS
AAGAAAGATGATTTCATGGA+TGG 0.509507 8_1:+18067583 Msa1182900:intron
AACACACACTTCTGGGGAGG+AGG 0.534134 8_1:-18067231 Msa1182910:intron
TGTTGGTCAGCTTCAGCAGA+AGG 0.540325 8_1:-18065444 Msa1182910:intron
AATTTCGACGTGGTGGTCAA+CGG 0.543936 8_1:+18067425 Msa1182900:intron
CACAAGCAGTGCAAATAACA+GGG 0.546740 8_1:+18065352 Msa1182900:intron
TATGTGTAAGGGACGATATG+AGG 0.546749 8_1:-18065396 Msa1182910:CDS
AAAGATGATTTCATGGATGG+AGG 0.549732 8_1:+18067586 Msa1182900:intron
TGATAGAAAATGATGAAAGA+AGG 0.549869 8_1:+18067557 Msa1182900:intron
ATGAGGATTACCGGTGCTCA+AGG 0.550612 8_1:-18065379 Msa1182910:CDS
AATCAAGCAACATGGTTGCA+AGG 0.564145 8_1:-18066825 Msa1182910:intron
GGTTTACGTGAGAGAGTGTG+AGG 0.596141 8_1:+18067613 Msa1182900:intron
ACAGGGGAATCCTTGAGCAC+CGG 0.609949 8_1:+18065369 Msa1182900:intron
TGAAGAGATGAAGATGATGA+TGG 0.610421 8_1:+18067506 Msa1182900:intron
ATTCTCTCTCCACCGCGCTG+AGG 0.620415 8_1:-18067405 Msa1182910:intron
GTTGGTCAGCTTCAGCAGAA+GGG 0.620779 8_1:-18065443 Msa1182910:intron
CTGCTTGTGTGGTTGGAAAG+CGG 0.632272 8_1:-18065338 Msa1182910:CDS
GGGAAGCTATTCCAAAGATG+AGG 0.641214 8_1:-18065311 Msa1182910:exon
TGTGGTTGGAAAGCGGCACA+GGG 0.641999 8_1:-18065331 Msa1182910:CDS
AACAACACACACTTCTGGGG+AGG 0.650153 8_1:-18067234 Msa1182910:five_prime_UTR
GATAACAACACACACTTCTG+GGG 0.668589 8_1:-18067237 Msa1182910:five_prime_UTR
TTCGACGTGGTGGTCAACGG+TGG 0.670969 8_1:+18067428 Msa1182900:intron
GAGAGAGAATTTCGACGTGG+TGG 0.679739 8_1:+18067418 Msa1182900:intron
ATATGAGAAATCAAGCAACA+TGG 0.704791 8_1:-18066833 Msa1182910:CDS
CTGATGATACCTCAGCGCGG+TGG 0.713867 8_1:+18067396 Msa1182900:intron
ACAAGCAGTGCAAATAACAG+GGG 0.745311 8_1:+18065353 Msa1182900:intron
GTGGAGAGAGAATTTCGACG+TGG 0.789373 8_1:+18067415 Msa1182900:intron
GAACTGATGATACCTCAGCG+CGG 0.855378 8_1:+18067393 Msa1182900:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATTAAAATGAGTATTTT+TGG + chr8_1:18065484-18065503 Msa1182900:intron 15.0%
!!! CAAAAATACTCATTTTAATT+TGG - chr8_1:18065482-18065501 Msa1182910:intron 15.0%
!!! GTTTTTGTAGTTTTAAATTT+TGG - chr8_1:18065550-18065569 Msa1182910:intron 15.0%
!! ACTCAAAATTCAAAATATAA+TGG + chr8_1:18065666-18065685 Msa1182900:intron 15.0%
!!! TTTTGAGTAAACATTTTTTT+TGG - chr8_1:18065678-18065697 Msa1182910:intron 15.0%
!!! TTCTGTTTTGTAAATTATAA+TGG - chr8_1:18065865-18065884 Msa1182910:intron 15.0%
!!! TTGTAAATTATAATGGATAA+TGG - chr8_1:18065872-18065891 Msa1182910:intron 15.0%
!!! CAAATTAACATTATAACTTT+TGG - chr8_1:18066291-18066310 Msa1182910:intron 15.0%
!! TTGATGTTTATAATTGAAAT+TGG - chr8_1:18066694-18066713 Msa1182910:intron 15.0%
!!! AAAACATAGTTTTAAATCAT+TGG + chr8_1:18067137-18067156 Msa1182900:intron 15.0%
!! TAAAACATTATTACTATTGT+AGG + chr8_1:18067340-18067359 Msa1182900:intron 15.0%
!!! TCATCATTTTCTATCAATTT+CGG - chr8_1:18065284-18065303 Msa1182910:three_prime_UTR 20.0%
!!! AATTTTCCAGAAAGATAAAT+GGG + chr8_1:18065710-18065729 Msa1182900:intron 20.0%
!!! TAATTTTCCAGAAAGATAAA+TGG + chr8_1:18065711-18065730 Msa1182900:intron 20.0%
!!! TTTTTCATATGTGAAAAGAT+TGG - chr8_1:18066108-18066127 Msa1182910:intron 20.0%
!!! TTTGGATACATTATAACTTT+TGG - chr8_1:18066309-18066328 Msa1182910:intron 20.0%
!! AAAAAAAATCGCATCATAAA+TGG - chr8_1:18066580-18066599 Msa1182910:intron 20.0%
!! AAAAAAAACCTTATTAGCAA+AGG + chr8_1:18066881-18066900 Msa1182900:intron 20.0%
!!! AAGGTTTTTTTTATGTTTGT+AGG - chr8_1:18066889-18066908 Msa1182910:intron 20.0%
!!! ATTTGATGATATAAAACTGT+AGG - chr8_1:18066930-18066949 Msa1182910:intron 20.0%
!! ATCTTGCTTTAATAATGATT+TGG - chr8_1:18067241-18067260 Msa1182910:five_prime_UTR 20.0%
!! ATTCAAAAAGATATGTGTAA+GGG - chr8_1:18067427-18067446 Msa1182910:five_prime_UTR 20.0%
! TGATAGAAAATGATGAAAGA+AGG + chr8_1:18065280-18065299 Msa1182900:intron 25.0%
! TATAAATCCGAAGATGAAAA+TGG + chr8_1:18065640-18065659 Msa1182900:intron 25.0%
! GAAATAGGTATGACAATTAA+AGG - chr8_1:18065792-18065811 Msa1182910:intron 25.0%
!! TATGTTAGCTTCAAATATGA+TGG - chr8_1:18065908-18065927 Msa1182910:intron 25.0%
!! ATGTTAGCTTCAAATATGAT+GGG - chr8_1:18065909-18065928 Msa1182910:intron 25.0%
!!! CATGTTATTTTCACATAGTT+TGG - chr8_1:18066222-18066241 Msa1182910:intron 25.0%
!!! CTGTTTTTAAGAAATGCTTT+TGG - chr8_1:18066252-18066271 Msa1182910:intron 25.0%
! ATAGAAAAAGCAACAAGAAA+AGG - chr8_1:18066468-18066487 Msa1182910:intron 25.0%
!! TTTTATGTTTGTAGGATTGA+CGG - chr8_1:18066897-18066916 Msa1182910:intron 25.0%
!! TTTTATAGTACTCTTGAATG+TGG - chr8_1:18067071-18067090 Msa1182910:intron 25.0%
!!! ATTGAAGAGATTATTTTGCA+AGG + chr8_1:18067371-18067390 Msa1182900:intron 25.0%
! CAAAATAATCTCTTCAATGT+TGG - chr8_1:18067373-18067392 Msa1182910:intron 25.0%
! GATTCAAAAAGATATGTGTA+AGG - chr8_1:18067426-18067445 Msa1182910:five_prime_UTR 25.0%
!! TTGATATCTATATCAGCTTT+TGG - chr8_1:18067568-18067587 Msa1182910:five_prime_UTR 25.0%
!! TGATATCTATATCAGCTTTT+GGG - chr8_1:18067569-18067588 Msa1182910:five_prime_UTR 25.0%
AAGAAAGATGATTTCATGGA+TGG + chr8_1:18065254-18065273 Msa1182900:intron 30.0%
GTAGAAGAAAGATGATTTCA+TGG + chr8_1:18065258-18065277 Msa1182900:intron 30.0%
!!! GATGATGGCTTTATTTTAGT+GGG + chr8_1:18065316-18065335 Msa1182900:intron 30.0%
!!! TGATGATGGCTTTATTTTAG+TGG + chr8_1:18065317-18065336 Msa1182900:intron 30.0%
! GATTCATCCATTTTCATCTT+CGG - chr8_1:18065630-18065649 Msa1182910:intron 30.0%
TCAAATCCCATTTATCTTTC+TGG - chr8_1:18065701-18065720 Msa1182910:intron 30.0%
TGCACATGAAAATAGAAACA+TGG + chr8_1:18065744-18065763 Msa1182900:intron 30.0%
TTTCATGTGCAATCAGAAAT+TGG - chr8_1:18065753-18065772 Msa1182910:intron 30.0%
GCTGATGAATTTCAAGAAAT+AGG - chr8_1:18065777-18065796 Msa1182910:intron 30.0%
ATATGAGAAATCAAGCAACA+TGG - chr8_1:18066001-18066020 Msa1182910:intron 30.0%
TGTTGTCATTGTCAATATGT+TGG - chr8_1:18066348-18066367 Msa1182910:intron 30.0%
ATTGAAATTGCAGTAGCTAT+TGG - chr8_1:18066416-18066435 Msa1182910:intron 30.0%
TTGAAATTGCAGTAGCTATT+GGG - chr8_1:18066417-18066436 Msa1182910:intron 30.0%
!!! TCTTGTTGCTTTTTCTATGA+AGG + chr8_1:18066466-18066485 Msa1182900:intron 30.0%
TAACTCATAATCAAGTTCTG+AGG - chr8_1:18066629-18066648 Msa1182910:intron 30.0%
!!! GCCTTTTGGTTCTATTTTTA+TGG - chr8_1:18066751-18066770 Msa1182910:intron 30.0%
GCCATAAAAATAGAACCAAA+AGG + chr8_1:18066755-18066774 Msa1182900:intron 30.0%
! GACTAAAGTGCTCAATATAA+TGG - chr8_1:18066839-18066858 Msa1182910:exon 30.0%
ATTGTAGGATTAAGCATCAA+AGG + chr8_1:18067325-18067344 Msa1182900:intron 30.0%
AAAGATGATTTCATGGATGG+AGG + chr8_1:18065251-18065270 Msa1182900:intron 35.0%
TGAAGAGATGAAGATGATGA+TGG + chr8_1:18065331-18065350 Msa1182900:intron 35.0%
!!! TGAATGAGTTGCTTTTGTAG+AGG + chr8_1:18065355-18065374 Msa1182900:intron 35.0%
TTTGGATGAAAGAGAGAGAA+AGG + chr8_1:18065466-18065485 Msa1182900:intron 35.0%
AGATAACAACACACACTTCT+GGG - chr8_1:18065596-18065615 Msa1182910:intron 35.0%
! AAATGCTTTTGGATGTGAGT+TGG - chr8_1:18066263-18066282 Msa1182910:intron 35.0%
!! ATAATCAAGTTCTGAGGTCA+TGG - chr8_1:18066635-18066654 Msa1182910:intron 35.0%
!!! TTGGTTCTATTTTTATGGCG+AGG - chr8_1:18066756-18066775 Msa1182910:intron 35.0%
AACCTGATAGCAATTAGCAA+AGG + chr8_1:18066802-18066821 Msa1182900:intron 35.0%
! TCAGGTTCTGATCTTAAGTT+AGG - chr8_1:18066815-18066834 Msa1182910:intron 35.0%
! CAGGTTCTGATCTTAAGTTA+GGG - chr8_1:18066816-18066835 Msa1182910:intron 35.0%
! AGGTTCTGATCTTAAGTTAG+GGG - chr8_1:18066817-18066836 Msa1182910:intron 35.0%
GACATGAGAAAACCAAACAT+TGG + chr8_1:18066962-18066981 Msa1182900:intron 35.0%
AAATGACCAATGAAGTTGTG+TGG - chr8_1:18067043-18067062 Msa1182910:intron 35.0%
TGATTTCATGGATGGAGGTT+GGG + chr8_1:18065246-18065265 Msa1182900:intron 40.0%
ATGATTTCATGGATGGAGGT+TGG + chr8_1:18065247-18065266 Msa1182900:intron 40.0%
!!! GATGGCTTTATTTTAGTGGG+AGG + chr8_1:18065313-18065332 Msa1182900:intron 40.0%
GAGATAACAACACACACTTC+TGG - chr8_1:18065595-18065614 Msa1182910:intron 40.0%
GATAACAACACACACTTCTG+GGG - chr8_1:18065597-18065616 Msa1182910:intron 40.0%
AATCAAGCAACATGGTTGCA+AGG - chr8_1:18066009-18066028 Msa1182910:intron 40.0%
TAGCAATTAGCAAAGGCAAG+TGG + chr8_1:18066795-18066814 Msa1182900:intron 40.0%
TGCCTTTGCTAATTGCTATC+AGG - chr8_1:18066797-18066816 Msa1182910:intron 40.0%
TTCACTCGCCTTTGCTAATA+AGG - chr8_1:18066870-18066889 Msa1182910:intron 40.0%
TTGTAGGATTGACGGTTTAG+CGG - chr8_1:18066905-18066924 Msa1182910:intron 40.0%
TGTAGGTCAGTACCAATGTT+TGG - chr8_1:18066947-18066966 Msa1182910:intron 40.0%
ATTCGAAGAGACACACACAT+TGG + chr8_1:18067001-18067020 Msa1182900:intron 40.0%
ACTCAACCACACAACTTCAT+TGG + chr8_1:18067052-18067071 Msa1182900:intron 40.0%
TATGTGTAAGGGACGATATG+AGG - chr8_1:18067438-18067457 Msa1182910:five_prime_UTR 40.0%
ACAAGCAGTGCAAATAACAG+GGG + chr8_1:18067484-18067503 Msa1182900:intron 40.0%
CACAAGCAGTGCAAATAACA+GGG + chr8_1:18067485-18067504 Msa1182900:intron 40.0%
ACACAAGCAGTGCAAATAAC+AGG + chr8_1:18067486-18067505 Msa1182900:intron 40.0%
! TGTTATTTGCACTGCTTGTG+TGG - chr8_1:18067485-18067504 Msa1182910:five_prime_UTR 40.0%
CTATTCCAAAGATGAGGTCT+TGG - chr8_1:18067529-18067548 Msa1182910:five_prime_UTR 40.0%
GATTTCATGGATGGAGGTTG+GGG + chr8_1:18065245-18065264 Msa1182900:intron 45.0%
!!! GGCTTTATTTTAGTGGGAGG+AGG + chr8_1:18065310-18065329 Msa1182900:intron 45.0%
AATTTCGACGTGGTGGTCAA+CGG + chr8_1:18065412-18065431 Msa1182900:intron 45.0%
! AGCGTTGTGTTTGTGCCTTT+TGG - chr8_1:18066737-18066756 Msa1182910:intron 45.0%
AGGGACGATATGAGGATTAC+CGG - chr8_1:18067446-18067465 Msa1182910:five_prime_UTR 45.0%
ATTTGCACTGCTTGTGTGGT+TGG - chr8_1:18067489-18067508 Msa1182910:five_prime_UTR 45.0%
GGGAAGCTATTCCAAAGATG+AGG - chr8_1:18067523-18067542 Msa1182910:five_prime_UTR 45.0%
ACTCTCCAAGACCTCATCTT+TGG + chr8_1:18067537-18067556 Msa1182900:intron 45.0%
!! ATATAAAAGAATAAAAAAAT+AGG + chr8_1:18067271-18067290 Msa1182900:intron 5.0%
!! GATTAATTAAAATAAAATAA+AGG + chr8_1:18067605-18067624 Msa1182900:intron 5.0%
TGAGGAAGAGAGAGAGAGAG+TGG + chr8_1:18065206-18065225 Msa1182900:intron 50.0%
! GGTTTACGTGAGAGAGTGTG+AGG + chr8_1:18065224-18065243 Msa1182900:intron 50.0%
GAGAGAGAATTTCGACGTGG+TGG + chr8_1:18065419-18065438 Msa1182900:intron 50.0%
GTGGAGAGAGAATTTCGACG+TGG + chr8_1:18065422-18065441 Msa1182900:intron 50.0%
GAACTGATGATACCTCAGCG+CGG + chr8_1:18065444-18065463 Msa1182900:intron 50.0%
! AACAACACACACTTCTGGGG+AGG - chr8_1:18065600-18065619 Msa1182910:intron 50.0%
TGTTGGTCAGCTTCAGCAGA+AGG - chr8_1:18067390-18067409 Msa1182910:intron 50.0%
GTTGGTCAGCTTCAGCAGAA+GGG - chr8_1:18067391-18067410 Msa1182910:intron 50.0%
ATGAGGATTACCGGTGCTCA+AGG - chr8_1:18067455-18067474 Msa1182910:five_prime_UTR 50.0%
CTGCTTGTGTGGTTGGAAAG+CGG - chr8_1:18067496-18067515 Msa1182910:five_prime_UTR 50.0%
! AACACACACTTCTGGGGAGG+AGG - chr8_1:18065603-18065622 Msa1182910:intron 55.0%
ACAGGGGAATCCTTGAGCAC+CGG + chr8_1:18067468-18067487 Msa1182900:intron 55.0%
TGTGGTTGGAAAGCGGCACA+GGG - chr8_1:18067503-18067522 Msa1182910:five_prime_UTR 55.0%
TTCGACGTGGTGGTCAACGG+TGG + chr8_1:18065409-18065428 Msa1182900:intron 60.0%
ATTCTCTCTCCACCGCGCTG+AGG - chr8_1:18065429-18065448 Msa1182910:CDS 60.0%
CTGATGATACCTCAGCGCGG+TGG + chr8_1:18065441-18065460 Msa1182900:intron 60.0%
GTGTGGTTGGAAAGCGGCAC+AGG - chr8_1:18067502-18067521 Msa1182910:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 18065196 18067660 18065196 ID=Msa1182910;Name=Msa1182910
chr8_1 mRNA 18065196 18067660 18065196 ID=Msa1182910-mRNA-1;Parent=Msa1182910;Name=Msa1182910-mRNA-1;_AED=0.11;_eAED=0.11;_QI=366|0.6|0.83|1|0|0|6|117|59
chr8_1 exon 18065196 18065456 18065196 ID=Msa1182910-mRNA-1:exon:6896;Parent=Msa1182910-mRNA-1
chr8_1 exon 18065938 18065945 18065938 ID=Msa1182910-mRNA-1:exon:6895;Parent=Msa1182910-mRNA-1
chr8_1 exon 18066826 18066869 18066826 ID=Msa1182910-mRNA-1:exon:6894;Parent=Msa1182910-mRNA-1
chr8_1 exon 18067058 18067087 18067058 ID=Msa1182910-mRNA-1:exon:6893;Parent=Msa1182910-mRNA-1
chr8_1 exon 18067232 18067296 18067232 ID=Msa1182910-mRNA-1:exon:6892;Parent=Msa1182910-mRNA-1
chr8_1 exon 18067406 18067660 18067406 ID=Msa1182910-mRNA-1:exon:6891;Parent=Msa1182910-mRNA-1
chr8_1 five_prime_UTR 18067406 18067660 18067406 ID=Msa1182910-mRNA-1:five_prime_utr;Parent=Msa1182910-mRNA-1
chr8_1 five_prime_UTR 18067232 18067296 18067232 ID=Msa1182910-mRNA-1:five_prime_utr;Parent=Msa1182910-mRNA-1
chr8_1 five_prime_UTR 18067058 18067087 18067058 ID=Msa1182910-mRNA-1:five_prime_utr;Parent=Msa1182910-mRNA-1
chr8_1 five_prime_UTR 18066854 18066869 18066854 ID=Msa1182910-mRNA-1:five_prime_utr;Parent=Msa1182910-mRNA-1
chr8_1 CDS 18066826 18066853 18066826 ID=Msa1182910-mRNA-1:cds;Parent=Msa1182910-mRNA-1
chr8_1 CDS 18065938 18065945 18065938 ID=Msa1182910-mRNA-1:cds;Parent=Msa1182910-mRNA-1
chr8_1 CDS 18065313 18065456 18065313 ID=Msa1182910-mRNA-1:cds;Parent=Msa1182910-mRNA-1
chr8_1 three_prime_UTR 18065196 18065312 18065196 ID=Msa1182910-mRNA-1:three_prime_utr;Parent=Msa1182910-mRNA-1
Gene Sequence

>Msa1182910

ATGAGAAATCAAGCAACATGGTTGCAAGGATTGACGCTTCAGCAGAAGGGTCTGATTGTTGTGATTCAAAAAGATATGTGTAAGGGACGATATGAGGATTACCGGTGCTCAAGGATTCCCCTGTTATTTGCACTGCTTGTGTGGTTGGAAAGCGGCACAGGGAAGCTATTCCAAAGATGA

Protein sequence

>Msa1182910

MRNQATWLQGLTLQQKGLIVVIQKDMCKGRYEDYRCSRIPLLFALLVWLESGTGKLFQR*