AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1311210


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1311210 MtrunA17_Chr4g0042641 85.714 28 4 0 32 59 17 44 1.16e-12 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATATCTATATCAGCTTT+TGG 0.255888 8_4:+7911735 Msa1311210:three_prime_UTR
TGATTTCATGGATGGAGGTT+GGG 0.325455 8_4:-7909410 Msa1311200:intron
GCTGATGAATTTCAAGAAAT+AGG 0.329504 8_4:+7909944 Msa1311210:five_prime_UTR
GAGATAACAACACACACTTC+TGG 0.338725 8_4:+7909762 Msa1311210:five_prime_UTR
ACACAAGCAGTGCAAATAAC+AGG 0.339261 8_4:-7911650 Msa1311200:intron
ACTCTCCAAGACCTCATCTT+TGG 0.363030 8_4:-7911701 Msa1311200:intron
GTAGAAGAAAGATGATTTCA+TGG 0.387224 8_4:-7909422 Msa1311200:intron
GATTTCATGGATGGAGGTTG+GGG 0.404674 8_4:-7909409 Msa1311200:intron
GATTAATTAAAATAAAATAA+AGG 0.418164 8_4:-7911769 Msa1311200:intron
ATGATTTCATGGATGGAGGT+TGG 0.434492 8_4:-7909411 Msa1311200:intron
GTGTGGTTGGAAAGCGGCAC+AGG 0.438612 8_4:+7911669 Msa1311210:CDS
CTATTCCAAAGATGAGGTCT+TGG 0.445964 8_4:+7911696 Msa1311210:exon
AGATAACAACACACACTTCT+GGG 0.464005 8_4:+7909763 Msa1311210:five_prime_UTR
ATTTGCACTGCTTGTGTGGT+TGG 0.468289 8_4:+7911656 Msa1311210:CDS
AGGGACGATATGAGGATTAC+CGG 0.476410 8_4:+7911613 Msa1311210:CDS
TGTTATTTGCACTGCTTGTG+TGG 0.507323 8_4:+7911652 Msa1311210:CDS
AAGAAAGATGATTTCATGGA+TGG 0.509507 8_4:-7909418 Msa1311200:intron
AACACACACTTCTGGGGAGG+AGG 0.534134 8_4:+7909770 Msa1311210:five_prime_UTR
TGTTGGTCAGCTTCAGCAGA+AGG 0.540325 8_4:+7911557 Msa1311210:intron
AATTTCGACGTGGTGGTCAA+CGG 0.543936 8_4:-7909576 Msa1311200:intron
CACAAGCAGTGCAAATAACA+GGG 0.546740 8_4:-7911649 Msa1311200:intron
TATGTGTAAGGGACGATATG+AGG 0.546749 8_4:+7911605 Msa1311210:CDS
AAAGATGATTTCATGGATGG+AGG 0.549732 8_4:-7909415 Msa1311200:intron
TGATAGAAAATGATGAAAGA+AGG 0.549869 8_4:-7909444 Msa1311200:intron
ATGAGGATTACCGGTGCTCA+AGG 0.550612 8_4:+7911622 Msa1311210:CDS
AATCAAGCAACATGGTTGCA+AGG 0.564145 8_4:+7910176 Msa1311210:CDS
GGTTTACGTGAGAGAGTGTG+AGG 0.596141 8_4:-7909388 Msa1311200:intron
ACAGGGGAATCCTTGAGCAC+CGG 0.609949 8_4:-7911632 Msa1311200:intron
TGAAGAGATGAAGATGATGA+TGG 0.610421 8_4:-7909495 Msa1311200:intron
ATTCTCTCTCCACCGCGCTG+AGG 0.620415 8_4:+7909596 Msa1311210:five_prime_UTR
GTTGGTCAGCTTCAGCAGAA+GGG 0.620779 8_4:+7911558 Msa1311210:intron
CTGCTTGTGTGGTTGGAAAG+CGG 0.632272 8_4:+7911663 Msa1311210:CDS
GGGAAGCTATTCCAAAGATG+AGG 0.641214 8_4:+7911690 Msa1311210:CDS
TGTGGTTGGAAAGCGGCACA+GGG 0.641999 8_4:+7911670 Msa1311210:CDS
AACAACACACACTTCTGGGG+AGG 0.650153 8_4:+7909767 Msa1311210:five_prime_UTR
GATAACAACACACACTTCTG+GGG 0.668589 8_4:+7909764 Msa1311210:five_prime_UTR
TTCGACGTGGTGGTCAACGG+TGG 0.670969 8_4:-7909573 Msa1311200:intron
GAGAGAGAATTTCGACGTGG+TGG 0.679739 8_4:-7909583 Msa1311200:intron
ATATGAGAAATCAAGCAACA+TGG 0.704791 8_4:+7910168 Msa1311210:exon
CTGATGATACCTCAGCGCGG+TGG 0.713867 8_4:-7909605 Msa1311200:intron
ACAAGCAGTGCAAATAACAG+GGG 0.745311 8_4:-7911648 Msa1311200:intron
GTGGAGAGAGAATTTCGACG+TGG 0.789373 8_4:-7909586 Msa1311200:intron
GAACTGATGATACCTCAGCG+CGG 0.855378 8_4:-7909608 Msa1311200:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATTAAAATGAGTATTTT+TGG - chr8_4:7909651-7909670 Msa1311200:intron 15.0%
!!! CAAAAATACTCATTTTAATT+TGG + chr8_4:7909649-7909668 Msa1311210:intron 15.0%
!!! GTTTTTGTAGTTTTAAATTT+TGG + chr8_4:7909717-7909736 Msa1311210:intron 15.0%
!! ACTCAAAATTCAAAATATAA+TGG - chr8_4:7909833-7909852 Msa1311200:intron 15.0%
!!! TTTTGAGTAAACATTTTTTT+TGG + chr8_4:7909845-7909864 Msa1311210:intron 15.0%
!!! TTCTGTTTTGTAAATTATAA+TGG + chr8_4:7910032-7910051 Msa1311210:intron 15.0%
!!! TTGTAAATTATAATGGATAA+TGG + chr8_4:7910039-7910058 Msa1311210:intron 15.0%
!!! CAAATTAACATTATAACTTT+TGG + chr8_4:7910458-7910477 Msa1311210:intron 15.0%
!! TTGATGTTTATAATTGAAAT+TGG + chr8_4:7910861-7910880 Msa1311210:intron 15.0%
!!! AAAACATAGTTTTAAATCAT+TGG - chr8_4:7911304-7911323 Msa1311200:intron 15.0%
!! TAAAACATTATTACTATTGT+AGG - chr8_4:7911507-7911526 Msa1311200:intron 15.0%
!!! TCATCATTTTCTATCAATTT+CGG + chr8_4:7909451-7909470 Msa1311210:five_prime_UTR 20.0%
!!! AATTTTCCAGAAAGATAAAT+GGG - chr8_4:7909877-7909896 Msa1311200:intron 20.0%
!!! TAATTTTCCAGAAAGATAAA+TGG - chr8_4:7909878-7909897 Msa1311200:intron 20.0%
!!! TTTTTCATATGTGAAAAGAT+TGG + chr8_4:7910275-7910294 Msa1311210:intron 20.0%
!!! TTTGGATACATTATAACTTT+TGG + chr8_4:7910476-7910495 Msa1311210:intron 20.0%
!! AAAAAAAATCGCATCATAAA+TGG + chr8_4:7910747-7910766 Msa1311210:intron 20.0%
!! AAAAAAAACCTTATTAGCAA+AGG - chr8_4:7911048-7911067 Msa1311200:intron 20.0%
!!! AAGGTTTTTTTTATGTTTGT+AGG + chr8_4:7911056-7911075 Msa1311210:intron 20.0%
!!! ATTTGATGATATAAAACTGT+AGG + chr8_4:7911097-7911116 Msa1311210:intron 20.0%
!! ATCTTGCTTTAATAATGATT+TGG + chr8_4:7911408-7911427 Msa1311210:intron 20.0%
!! ATTCAAAAAGATATGTGTAA+GGG + chr8_4:7911594-7911613 Msa1311210:CDS 20.0%
! TGATAGAAAATGATGAAAGA+AGG - chr8_4:7909447-7909466 Msa1311200:intron 25.0%
! TATAAATCCGAAGATGAAAA+TGG - chr8_4:7909807-7909826 Msa1311200:intron 25.0%
! GAAATAGGTATGACAATTAA+AGG + chr8_4:7909959-7909978 Msa1311210:intron 25.0%
!! TATGTTAGCTTCAAATATGA+TGG + chr8_4:7910075-7910094 Msa1311210:intron 25.0%
!! ATGTTAGCTTCAAATATGAT+GGG + chr8_4:7910076-7910095 Msa1311210:intron 25.0%
!!! CATGTTATTTTCACATAGTT+TGG + chr8_4:7910389-7910408 Msa1311210:intron 25.0%
!!! CTGTTTTTAAGAAATGCTTT+TGG + chr8_4:7910419-7910438 Msa1311210:intron 25.0%
! ATAGAAAAAGCAACAAGAAA+AGG + chr8_4:7910635-7910654 Msa1311210:intron 25.0%
!! TTTTATGTTTGTAGGATTGA+CGG + chr8_4:7911064-7911083 Msa1311210:intron 25.0%
!! TTTTATAGTACTCTTGAATG+TGG + chr8_4:7911238-7911257 Msa1311210:intron 25.0%
!!! ATTGAAGAGATTATTTTGCA+AGG - chr8_4:7911538-7911557 Msa1311200:intron 25.0%
! CAAAATAATCTCTTCAATGT+TGG + chr8_4:7911540-7911559 Msa1311210:intron 25.0%
! GATTCAAAAAGATATGTGTA+AGG + chr8_4:7911593-7911612 Msa1311210:CDS 25.0%
!! TTGATATCTATATCAGCTTT+TGG + chr8_4:7911735-7911754 Msa1311210:three_prime_UTR 25.0%
!! TGATATCTATATCAGCTTTT+GGG + chr8_4:7911736-7911755 Msa1311210:three_prime_UTR 25.0%
AAGAAAGATGATTTCATGGA+TGG - chr8_4:7909421-7909440 Msa1311200:intron 30.0%
GTAGAAGAAAGATGATTTCA+TGG - chr8_4:7909425-7909444 Msa1311200:intron 30.0%
!!! GATGATGGCTTTATTTTAGT+GGG - chr8_4:7909483-7909502 Msa1311200:intron 30.0%
!!! TGATGATGGCTTTATTTTAG+TGG - chr8_4:7909484-7909503 Msa1311200:intron 30.0%
! GATTCATCCATTTTCATCTT+CGG + chr8_4:7909797-7909816 Msa1311210:intron 30.0%
TCAAATCCCATTTATCTTTC+TGG + chr8_4:7909868-7909887 Msa1311210:intron 30.0%
TGCACATGAAAATAGAAACA+TGG - chr8_4:7909911-7909930 Msa1311200:intron 30.0%
TTTCATGTGCAATCAGAAAT+TGG + chr8_4:7909920-7909939 Msa1311210:intron 30.0%
GCTGATGAATTTCAAGAAAT+AGG + chr8_4:7909944-7909963 Msa1311210:five_prime_UTR 30.0%
ATATGAGAAATCAAGCAACA+TGG + chr8_4:7910168-7910187 Msa1311210:exon 30.0%
TGTTGTCATTGTCAATATGT+TGG + chr8_4:7910515-7910534 Msa1311210:intron 30.0%
ATTGAAATTGCAGTAGCTAT+TGG + chr8_4:7910583-7910602 Msa1311210:intron 30.0%
TTGAAATTGCAGTAGCTATT+GGG + chr8_4:7910584-7910603 Msa1311210:intron 30.0%
!!! TCTTGTTGCTTTTTCTATGA+AGG - chr8_4:7910633-7910652 Msa1311200:intron 30.0%
TAACTCATAATCAAGTTCTG+AGG + chr8_4:7910796-7910815 Msa1311210:intron 30.0%
!!! GCCTTTTGGTTCTATTTTTA+TGG + chr8_4:7910918-7910937 Msa1311210:intron 30.0%
GCCATAAAAATAGAACCAAA+AGG - chr8_4:7910922-7910941 Msa1311200:intron 30.0%
! GACTAAAGTGCTCAATATAA+TGG + chr8_4:7911006-7911025 Msa1311210:intron 30.0%
ATTGTAGGATTAAGCATCAA+AGG - chr8_4:7911492-7911511 Msa1311200:intron 30.0%
AAAGATGATTTCATGGATGG+AGG - chr8_4:7909418-7909437 Msa1311200:intron 35.0%
TGAAGAGATGAAGATGATGA+TGG - chr8_4:7909498-7909517 Msa1311200:intron 35.0%
!!! TGAATGAGTTGCTTTTGTAG+AGG - chr8_4:7909522-7909541 Msa1311200:intron 35.0%
TTTGGATGAAAGAGAGAGAA+AGG - chr8_4:7909633-7909652 Msa1311200:intron 35.0%
AGATAACAACACACACTTCT+GGG + chr8_4:7909763-7909782 Msa1311210:five_prime_UTR 35.0%
! AAATGCTTTTGGATGTGAGT+TGG + chr8_4:7910430-7910449 Msa1311210:intron 35.0%
!! ATAATCAAGTTCTGAGGTCA+TGG + chr8_4:7910802-7910821 Msa1311210:intron 35.0%
!!! TTGGTTCTATTTTTATGGCG+AGG + chr8_4:7910923-7910942 Msa1311210:intron 35.0%
AACCTGATAGCAATTAGCAA+AGG - chr8_4:7910969-7910988 Msa1311200:intron 35.0%
! TCAGGTTCTGATCTTAAGTT+AGG + chr8_4:7910982-7911001 Msa1311210:intron 35.0%
! CAGGTTCTGATCTTAAGTTA+GGG + chr8_4:7910983-7911002 Msa1311210:intron 35.0%
! AGGTTCTGATCTTAAGTTAG+GGG + chr8_4:7910984-7911003 Msa1311210:intron 35.0%
GACATGAGAAAACCAAACAT+TGG - chr8_4:7911129-7911148 Msa1311200:intron 35.0%
AAATGACCAATGAAGTTGTG+TGG + chr8_4:7911210-7911229 Msa1311210:intron 35.0%
TGATTTCATGGATGGAGGTT+GGG - chr8_4:7909413-7909432 Msa1311200:intron 40.0%
ATGATTTCATGGATGGAGGT+TGG - chr8_4:7909414-7909433 Msa1311200:intron 40.0%
!!! GATGGCTTTATTTTAGTGGG+AGG - chr8_4:7909480-7909499 Msa1311200:intron 40.0%
GAGATAACAACACACACTTC+TGG + chr8_4:7909762-7909781 Msa1311210:five_prime_UTR 40.0%
GATAACAACACACACTTCTG+GGG + chr8_4:7909764-7909783 Msa1311210:five_prime_UTR 40.0%
AATCAAGCAACATGGTTGCA+AGG + chr8_4:7910176-7910195 Msa1311210:CDS 40.0%
TAGCAATTAGCAAAGGCAAG+TGG - chr8_4:7910962-7910981 Msa1311200:intron 40.0%
TGCCTTTGCTAATTGCTATC+AGG + chr8_4:7910964-7910983 Msa1311210:intron 40.0%
TTCACTCGCCTTTGCTAATA+AGG + chr8_4:7911037-7911056 Msa1311210:intron 40.0%
TTGTAGGATTGACGGTTTAG+CGG + chr8_4:7911072-7911091 Msa1311210:intron 40.0%
TGTAGGTCAGTACCAATGTT+TGG + chr8_4:7911114-7911133 Msa1311210:intron 40.0%
ATTCGAAGAGACACACACAT+TGG - chr8_4:7911168-7911187 Msa1311200:intron 40.0%
ACTCAACCACACAACTTCAT+TGG - chr8_4:7911219-7911238 Msa1311200:intron 40.0%
TATGTGTAAGGGACGATATG+AGG + chr8_4:7911605-7911624 Msa1311210:CDS 40.0%
ACAAGCAGTGCAAATAACAG+GGG - chr8_4:7911651-7911670 Msa1311200:intron 40.0%
CACAAGCAGTGCAAATAACA+GGG - chr8_4:7911652-7911671 Msa1311200:intron 40.0%
ACACAAGCAGTGCAAATAAC+AGG - chr8_4:7911653-7911672 Msa1311200:intron 40.0%
! TGTTATTTGCACTGCTTGTG+TGG + chr8_4:7911652-7911671 Msa1311210:CDS 40.0%
CTATTCCAAAGATGAGGTCT+TGG + chr8_4:7911696-7911715 Msa1311210:exon 40.0%
GATTTCATGGATGGAGGTTG+GGG - chr8_4:7909412-7909431 Msa1311200:intron 45.0%
!!! GGCTTTATTTTAGTGGGAGG+AGG - chr8_4:7909477-7909496 Msa1311200:intron 45.0%
AATTTCGACGTGGTGGTCAA+CGG - chr8_4:7909579-7909598 Msa1311200:intron 45.0%
! AGCGTTGTGTTTGTGCCTTT+TGG + chr8_4:7910904-7910923 Msa1311210:intron 45.0%
AGGGACGATATGAGGATTAC+CGG + chr8_4:7911613-7911632 Msa1311210:CDS 45.0%
ATTTGCACTGCTTGTGTGGT+TGG + chr8_4:7911656-7911675 Msa1311210:CDS 45.0%
GGGAAGCTATTCCAAAGATG+AGG + chr8_4:7911690-7911709 Msa1311210:CDS 45.0%
ACTCTCCAAGACCTCATCTT+TGG - chr8_4:7911704-7911723 Msa1311200:intron 45.0%
!! ATATAAAAGAATAAAAAAAT+AGG - chr8_4:7911438-7911457 Msa1311200:intron 5.0%
!! GATTAATTAAAATAAAATAA+AGG - chr8_4:7911772-7911791 Msa1311200:intron 5.0%
TGAGGAAGAGAGAGAGAGAG+TGG - chr8_4:7909373-7909392 Msa1311200:intron 50.0%
! GGTTTACGTGAGAGAGTGTG+AGG - chr8_4:7909391-7909410 Msa1311200:intron 50.0%
GAGAGAGAATTTCGACGTGG+TGG - chr8_4:7909586-7909605 Msa1311200:intron 50.0%
GTGGAGAGAGAATTTCGACG+TGG - chr8_4:7909589-7909608 Msa1311200:intron 50.0%
GAACTGATGATACCTCAGCG+CGG - chr8_4:7909611-7909630 Msa1311200:intron 50.0%
! AACAACACACACTTCTGGGG+AGG + chr8_4:7909767-7909786 Msa1311210:five_prime_UTR 50.0%
TGTTGGTCAGCTTCAGCAGA+AGG + chr8_4:7911557-7911576 Msa1311210:intron 50.0%
GTTGGTCAGCTTCAGCAGAA+GGG + chr8_4:7911558-7911577 Msa1311210:intron 50.0%
ATGAGGATTACCGGTGCTCA+AGG + chr8_4:7911622-7911641 Msa1311210:CDS 50.0%
CTGCTTGTGTGGTTGGAAAG+CGG + chr8_4:7911663-7911682 Msa1311210:CDS 50.0%
! AACACACACTTCTGGGGAGG+AGG + chr8_4:7909770-7909789 Msa1311210:five_prime_UTR 55.0%
ACAGGGGAATCCTTGAGCAC+CGG - chr8_4:7911635-7911654 Msa1311200:intron 55.0%
TGTGGTTGGAAAGCGGCACA+GGG + chr8_4:7911670-7911689 Msa1311210:CDS 55.0%
TTCGACGTGGTGGTCAACGG+TGG - chr8_4:7909576-7909595 Msa1311200:intron 60.0%
ATTCTCTCTCCACCGCGCTG+AGG + chr8_4:7909596-7909615 Msa1311210:five_prime_UTR 60.0%
CTGATGATACCTCAGCGCGG+TGG - chr8_4:7909608-7909627 Msa1311200:intron 60.0%
GTGTGGTTGGAAAGCGGCAC+AGG + chr8_4:7911669-7911688 Msa1311210:CDS 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 7909363 7911827 7909363 ID=Msa1311210;Name=Msa1311210
chr8_4 mRNA 7909363 7911827 7909363 ID=Msa1311210-mRNA-1;Parent=Msa1311210;Name=Msa1311210-mRNA-1;_AED=0.11;_eAED=0.11;_QI=366|0.6|0.83|1|0|0|6|117|59
chr8_4 exon 7909363 7909617 7909363 ID=Msa1311210-mRNA-1:exon:2911;Parent=Msa1311210-mRNA-1
chr8_4 exon 7909727 7909791 7909727 ID=Msa1311210-mRNA-1:exon:2912;Parent=Msa1311210-mRNA-1
chr8_4 exon 7909936 7909965 7909936 ID=Msa1311210-mRNA-1:exon:2913;Parent=Msa1311210-mRNA-1
chr8_4 exon 7910154 7910197 7910154 ID=Msa1311210-mRNA-1:exon:2914;Parent=Msa1311210-mRNA-1
chr8_4 exon 7911078 7911085 7911078 ID=Msa1311210-mRNA-1:exon:2915;Parent=Msa1311210-mRNA-1
chr8_4 exon 7911567 7911827 7911567 ID=Msa1311210-mRNA-1:exon:2916;Parent=Msa1311210-mRNA-1
chr8_4 five_prime_UTR 7909363 7909617 7909363 ID=Msa1311210-mRNA-1:five_prime_utr;Parent=Msa1311210-mRNA-1
chr8_4 five_prime_UTR 7909727 7909791 7909727 ID=Msa1311210-mRNA-1:five_prime_utr;Parent=Msa1311210-mRNA-1
chr8_4 five_prime_UTR 7909936 7909965 7909936 ID=Msa1311210-mRNA-1:five_prime_utr;Parent=Msa1311210-mRNA-1
chr8_4 five_prime_UTR 7910154 7910169 7910154 ID=Msa1311210-mRNA-1:five_prime_utr;Parent=Msa1311210-mRNA-1
chr8_4 CDS 7910170 7910197 7910170 ID=Msa1311210-mRNA-1:cds;Parent=Msa1311210-mRNA-1
chr8_4 CDS 7911078 7911085 7911078 ID=Msa1311210-mRNA-1:cds;Parent=Msa1311210-mRNA-1
chr8_4 CDS 7911567 7911710 7911567 ID=Msa1311210-mRNA-1:cds;Parent=Msa1311210-mRNA-1
chr8_4 three_prime_UTR 7911711 7911827 7911711 ID=Msa1311210-mRNA-1:three_prime_utr;Parent=Msa1311210-mRNA-1
Gene Sequence

>Msa1311210

ATGAGAAATCAAGCAACATGGTTGCAAGGATTGACGCTTCAGCAGAAGGGTCTGATTGTTGTGATTCAAAAAGATATGTGTAAGGGACGATATGAGGATTACCGGTGCTCAAGGATTCCCCTGTTATTTGCACTGCTTGTGTGGTTGGAAAGCGGCACAGGGAAGCTATTCCAAAGATGA

Protein sequence

>Msa1311210

MRNQATWLQGLTLQQKGLIVVIQKDMCKGRYEDYRCSRIPLLFALLVWLESGTGKLFQR*