AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211630 MtrunA17_Chr4g0060351 100.000 152 0 0 1 152 1 152 1.40e-113 318
Msa1211630 MtrunA17_Chr8g0362321 97.368 152 4 0 1 152 1 152 1.53e-111 313
Msa1211630 MtrunA17_Chr5g0432831 45.890 146 74 1 5 150 2 142 2.67e-45 145
Msa1211630 MtrunA17_Chr3g0108261 45.890 146 74 1 5 150 2 142 3.12e-45 145
Msa1211630 MtrunA17_Chr7g0275361 45.205 146 75 1 5 150 2 142 7.54e-45 144
Msa1211630 MtrunA17_Chr1g0154011 45.890 146 74 1 5 150 2 142 1.11e-44 143
Msa1211630 MtrunA17_Chr8g0369311 46.575 146 73 1 5 150 58 198 3.06e-44 144
Msa1211630 MtrunA17_Chr4g0037011 44.521 146 76 1 5 150 2 142 1.77e-43 140
Msa1211630 MtrunA17_Chr4g0038891 46.853 143 75 1 2 144 30 171 1.33e-40 134
Msa1211630 MtrunA17_Chr5g0396451 45.652 138 74 1 7 144 35 171 9.55e-39 129
Msa1211630 MtrunA17_Chr2g0316201 40.146 137 82 0 7 143 8 144 2.46e-38 127
Msa1211630 MtrunA17_Chr4g0053191 42.208 154 82 2 1 147 1 154 6.16e-38 127
Msa1211630 MtrunA17_Chr5g0401831 41.558 154 83 2 1 147 1 154 1.62e-37 125
Msa1211630 MtrunA17_Chr2g0316211 41.085 129 76 0 8 136 9 137 3.51e-37 124
Msa1211630 MtrunA17_Chr4g0031181 39.416 137 70 1 25 148 26 162 2.08e-34 118
Msa1211630 MtrunA17_Chr3g0139901 38.732 142 83 3 5 142 4 145 1.27e-33 115
Msa1211630 MtrunA17_Chr1g0199951 41.958 143 81 2 2 143 39 180 9.35e-33 114
Msa1211630 MtrunA17_Chr2g0328001 36.496 137 74 1 25 148 26 162 1.37e-30 108
Msa1211630 MtrunA17_Chr7g0254701 36.029 136 87 0 7 142 13 148 1.93e-29 108
Msa1211630 MtrunA17_Chr8g0378601 34.848 132 86 0 7 138 25 156 2.41e-28 102
Msa1211630 MtrunA17_Chr3g0131871 34.507 142 84 2 10 142 1 142 9.87e-27 99.4
Msa1211630 MtrunA17_Chr8g0348701 30.201 149 98 3 1 147 1 145 2.19e-25 95.5
Msa1211630 MtrunA17_Chr4g0059291 29.530 149 99 3 1 147 1 145 5.63e-25 94.7
Msa1211630 MtrunA17_Chr8g0387131 32.000 150 94 4 1 147 1 145 2.87e-23 90.1
Msa1211630 MtrunA17_Chr5g0403591 27.778 144 103 1 8 151 33 175 4.34e-21 84.3
Msa1211630 MtrunA17_Chr8g0376111 32.857 140 82 4 7 134 43 182 1.14e-17 77.8
Msa1211630 MtrunA17_Chr4g0045101 33.784 148 85 5 7 141 37 184 2.04e-17 77.0
Msa1211630 MtrunA17_Chr7g0233611 33.571 140 81 4 7 134 38 177 5.99e-17 75.9
Msa1211630 MtrunA17_Chr8g0372391 36.792 106 65 2 1 105 12 116 8.46e-17 72.8
Msa1211630 MtrunA17_Chr7g0233631 31.410 156 89 5 7 150 38 187 1.55e-16 74.7
Msa1211630 MtrunA17_Chr8g0345331 31.092 119 73 3 7 120 8 122 1.01e-15 71.6
Msa1211630 MtrunA17_Chr5g0404931 35.849 106 66 2 1 105 12 116 1.09e-15 69.7
Msa1211630 MtrunA17_Chr1g0186291 26.667 105 63 2 46 149 30 121 1.57e-12 61.2
Msa1211630 MtrunA17_Chr1g0198021 31.973 147 86 6 7 139 138 284 3.14e-12 62.8
Msa1211630 MtrunA17_Chr4g0068301 29.457 129 81 4 1 124 7 130 4.05e-12 62.4
Msa1211630 MtrunA17_Chr2g0317631 31.148 122 74 4 7 118 307 428 9.34e-12 61.6
Msa1211630 MtrunA17_Chr8g0376201 30.137 146 88 5 7 138 193 338 2.68e-11 60.5
Msa1211630 MtrunA17_Chr1g0211131 30.081 123 74 4 7 118 823 944 4.33e-11 60.1
Msa1211630 MtrunA17_Chr2g0282211 29.268 123 75 3 7 118 682 803 5.12e-11 59.7
Msa1211630 MtrunA17_Chr7g0222941 28.689 122 77 3 7 118 91 212 9.40e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211630 AT2G02760.1 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1211630 AT2G02760.2 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1211630 AT2G02760.4 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1211630 AT2G02760.3 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1211630 AT1G14400.1 97.368 152 4 0 1 152 1 152 1.69e-110 310
Msa1211630 AT1G14400.2 97.368 152 4 0 1 152 1 152 1.69e-110 310
Msa1211630 AT5G62540.1 86.000 150 21 0 1 150 1 150 1.96e-98 280
Msa1211630 AT4G27960.1 45.205 146 75 1 5 150 2 142 4.64e-45 145
Msa1211630 AT1G64230.2 44.521 146 76 1 5 150 2 142 1.15e-44 144
Msa1211630 AT1G64230.1 44.521 146 76 1 5 150 2 142 1.15e-44 144
Msa1211630 AT5G53300.2 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1211630 AT5G53300.4 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1211630 AT5G53300.1 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1211630 AT4G27960.2 45.205 146 75 1 5 150 32 172 3.41e-44 144
Msa1211630 AT3G08690.2 44.521 146 76 1 5 150 2 142 5.24e-44 142
Msa1211630 AT3G08690.1 44.521 146 76 1 5 150 2 142 5.24e-44 142
Msa1211630 AT2G16740.1 43.151 146 78 1 5 150 2 142 7.27e-44 142
Msa1211630 AT5G41700.5 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1211630 AT5G41700.2 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1211630 AT5G41700.1 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1211630 AT3G08700.2 46.259 147 73 2 5 150 2 143 3.56e-43 140
Msa1211630 AT3G08700.1 46.259 147 73 2 5 150 2 143 3.56e-43 140
Msa1211630 AT5G56150.1 44.521 146 76 1 5 150 2 142 6.44e-43 139
Msa1211630 AT1G64230.3 43.226 155 74 3 5 150 2 151 1.13e-41 136
Msa1211630 AT5G41700.4 43.537 147 77 2 5 150 2 143 1.33e-41 136
Msa1211630 AT3G20060.1 46.429 140 74 1 7 146 39 177 2.80e-39 131
Msa1211630 AT1G50490.1 47.143 140 73 1 7 146 38 176 2.08e-38 129
Msa1211630 AT1G16890.2 40.146 137 82 0 7 143 8 144 4.09e-38 127
Msa1211630 AT3G20060.2 49.194 124 62 1 23 146 10 132 6.47e-38 126
Msa1211630 AT1G78870.2 40.146 137 82 0 7 143 8 144 8.97e-38 126
Msa1211630 AT1G64230.5 34.574 188 76 2 5 150 2 184 2.25e-36 124
Msa1211630 AT1G64230.4 34.574 188 76 2 5 150 2 184 2.25e-36 124
Msa1211630 AT1G16890.3 38.356 146 81 1 7 143 8 153 1.55e-35 121
Msa1211630 AT3G57870.1 40.000 145 80 2 5 142 6 150 9.72e-35 119
Msa1211630 AT5G59300.1 39.416 137 70 1 25 148 26 162 2.18e-33 115
Msa1211630 AT5G25760.1 39.130 138 80 3 5 138 4 141 5.54e-33 114
Msa1211630 AT5G25760.2 39.130 138 80 3 5 138 4 141 5.54e-33 114
Msa1211630 AT3G46460.1 39.416 137 70 1 25 148 26 162 1.08e-32 114
Msa1211630 AT5G41700.3 44.340 106 59 0 5 110 2 107 1.54e-32 113
Msa1211630 AT3G55380.1 38.686 137 71 1 25 148 27 163 2.44e-32 113
Msa1211630 AT5G53300.3 45.545 101 55 0 5 105 2 102 3.65e-32 110
Msa1211630 AT5G56150.2 43.519 108 61 0 5 112 2 109 1.49e-31 109
Msa1211630 AT3G13550.1 35.766 137 88 0 7 143 39 175 2.14e-31 111
Msa1211630 AT1G36340.2 46.087 115 62 0 31 145 34 148 1.36e-30 108
Msa1211630 AT1G36340.1 46.087 115 62 0 31 145 34 148 1.36e-30 108
Msa1211630 AT5G05080.2 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1211630 AT5G05080.3 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1211630 AT5G05080.1 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1211630 AT5G05080.4 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1211630 AT1G16890.1 42.593 108 62 0 36 143 4 111 4.70e-30 105
Msa1211630 AT1G78870.3 40.952 105 62 0 7 111 8 112 6.87e-30 105
Msa1211630 AT1G78870.4 42.593 108 62 0 36 143 4 111 1.11e-29 105
Msa1211630 AT1G78870.1 44.118 102 56 1 36 136 4 105 7.57e-28 100
Msa1211630 AT5G50870.1 35.211 142 83 2 10 142 1 142 2.03e-27 101
Msa1211630 AT3G13550.2 38.393 112 69 0 7 118 39 150 1.36e-26 98.6
Msa1211630 AT2G46030.5 30.872 149 97 3 1 147 1 145 1.72e-26 99.0
Msa1211630 AT2G46030.1 30.872 149 97 3 1 147 1 145 1.72e-26 99.0
Msa1211630 AT3G24515.1 34.641 153 87 2 8 147 10 162 4.11e-26 102
Msa1211630 AT3G46460.3 39.823 113 55 1 49 148 8 120 4.97e-26 95.9
Msa1211630 AT3G46460.2 39.823 113 55 1 49 148 8 120 4.97e-26 95.9
Msa1211630 AT5G50870.2 37.500 128 71 2 10 128 1 128 7.30e-26 97.8
Msa1211630 AT2G46030.3 30.872 149 97 3 1 147 73 217 1.22e-25 98.2
Msa1211630 AT1G63800.1 34.000 150 91 4 1 147 1 145 1.52e-25 96.3
Msa1211630 AT5G41340.1 34.000 150 91 4 1 147 1 145 3.84e-25 95.5
Msa1211630 AT3G55380.2 30.409 171 72 2 25 148 27 197 7.36e-25 95.1
Msa1211630 AT2G46030.4 33.333 120 78 2 30 147 29 148 1.32e-24 94.0
Msa1211630 AT2G46030.2 33.333 120 78 2 30 147 29 148 1.32e-24 94.0
Msa1211630 AT1G63800.2 37.037 108 66 2 42 147 23 130 3.05e-24 92.8
Msa1211630 AT3G24515.3 38.393 112 65 1 40 147 76 187 1.23e-22 92.8
Msa1211630 AT5G41340.2 36.275 102 63 2 48 147 9 110 5.72e-22 86.3
Msa1211630 AT4G36800.2 28.472 144 102 1 8 151 34 176 2.06e-21 85.9
Msa1211630 AT4G36800.1 28.472 144 102 1 8 151 34 176 2.06e-21 85.9
Msa1211630 AT2G18600.1 30.435 138 95 1 8 145 35 171 2.91e-21 85.5
Msa1211630 AT2G18600.2 30.435 138 95 1 8 145 35 171 2.91e-21 85.5
Msa1211630 AT2G32790.1 32.877 146 93 2 1 143 26 169 4.84e-19 79.3
Msa1211630 AT1G45050.1 36.449 107 66 2 1 106 12 117 3.84e-16 71.2
Msa1211630 AT1G75440.1 35.849 106 66 2 1 105 12 116 6.60e-16 70.9
Msa1211630 AT4G36410.1 33.962 106 68 2 1 105 12 116 1.49e-15 69.7
Msa1211630 AT5G50430.1 30.597 134 81 4 7 135 8 134 1.79e-15 71.2
Msa1211630 AT5G50430.2 30.597 134 81 4 7 135 8 134 1.79e-15 71.2
Msa1211630 AT5G42990.1 34.579 107 68 2 1 106 12 117 3.60e-15 68.9
Msa1211630 AT1G17280.9 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.7 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.6 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.2 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.5 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.3 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.1 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.8 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1211630 AT1G17280.4 27.273 121 81 1 7 120 8 128 2.99e-14 68.2
Msa1211630 AT1G53025.2 31.081 148 81 5 7 133 274 421 6.35e-14 68.2
Msa1211630 AT1G53025.1 31.081 148 81 5 7 133 274 421 6.63e-14 68.2
Msa1211630 AT3G17000.1 28.467 137 85 4 4 135 11 139 1.81e-12 63.5
Msa1211630 AT1G45050.2 35.870 92 57 2 1 91 12 102 6.20e-12 59.3
Msa1211630 AT2G16920.1 30.894 123 73 4 7 118 853 974 9.28e-12 62.4
Msa1211630 AT2G16920.2 30.894 123 73 4 7 118 853 974 9.28e-12 62.4
Msa1211630 AT1G53023.1 27.703 148 86 4 7 133 60 207 1.89e-11 60.8

Find 86 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGAGTTTGCAGGAGAATT+TGG 0.151098 8_1:-71345042 None:intergenic
CTAATAAAATCATTGAATTT+TGG 0.164075 8_1:+71345517 Msa1211630:three_prime_UTR
GGCGTGGTTGGTTTCGATTC+TGG 0.209053 8_1:-71341326 None:intergenic
CTGTGGAATGCTGTTATATT+TGG 0.236507 8_1:+71341970 Msa1211630:CDS
CGGCCTGTGAAGGCAGGATT+TGG 0.269187 8_1:+71345173 Msa1211630:three_prime_UTR
CATTCCTTGTTATGAATGTT+AGG 0.279486 8_1:+71345229 Msa1211630:three_prime_UTR
CAGAGTTTGCAGGAGAATTT+GGG 0.319027 8_1:-71345041 None:intergenic
TTCATCTTATCTTTCATTCA+TGG 0.344421 8_1:+71345252 Msa1211630:three_prime_UTR
CTGAATTGGTTATGATCATA+AGG 0.358906 8_1:+71345374 Msa1211630:three_prime_UTR
GACTGACCCGTTAGTGTGTT+TGG 0.359010 8_1:-71341440 None:intergenic
GTTAAAATGAGGAATAAATC+TGG 0.363070 8_1:+71345487 Msa1211630:three_prime_UTR
CGTACAGTGGGTGGCTTGTT+CGG 0.389561 8_1:-71343367 None:intergenic
TAAGTTGTACTTGTCTGAAT+TGG 0.392807 8_1:+71345360 Msa1211630:three_prime_UTR
TACTGTTTGGATGAAACATT+CGG 0.396289 8_1:-71343399 None:intergenic
AGGAATTAGTGGTGCTCCTC+AGG 0.412828 8_1:+71341933 Msa1211630:CDS
TGCAGATGGAAGTATTTGTC+TGG 0.414767 8_1:+71343502 Msa1211630:CDS
CAGTGCAAACAGAATTCAAC+CGG 0.417063 8_1:-71345408 None:intergenic
GGTCATCTTGATATGCTTGT+TGG 0.429544 8_1:+71345150 Msa1211630:three_prime_UTR
ATGGTGAATTTAGAATTTAA+AGG 0.441741 8_1:-71341356 None:intergenic
ACTGACCCGTTAGTGTGTTT+GGG 0.443888 8_1:-71341439 None:intergenic
AAAGCAAGGGCGGGCGGCTA+TGG 0.451384 8_1:-71341375 None:intergenic
ACTGTTTGGATGAAACATTC+GGG 0.455692 8_1:-71343398 None:intergenic
GACCCACCTGCAGGAATTAG+TGG 0.459602 8_1:+71341922 Msa1211630:CDS
TGTGGAATGCTGTTATATTT+GGG 0.459892 8_1:+71341971 Msa1211630:CDS
TTTGCAGGAGAATTTGGGTT+GGG 0.465291 8_1:-71345036 None:intergenic
TCTCTCCCAAACACACTAAC+GGG 0.478747 8_1:+71341434 Msa1211630:five_prime_UTR
TGCGATTCTCACTTCTATCC+AGG 0.480462 8_1:+71343565 Msa1211630:CDS
TGAGTTTGCATGACTCTCAT+AGG 0.483915 8_1:-71345208 None:intergenic
GATAATCCTCGGAAAATTGA+AGG 0.484718 8_1:-71343345 None:intergenic
AGAAATGATATAAGAGTGGA+AGG 0.486337 8_1:+71341289 Msa1211630:five_prime_UTR
AAAGGCAGGCGTGGCGTGGT+TGG 0.487277 8_1:-71341338 None:intergenic
CGAGCCGCTTCAGAGTTTGC+AGG 0.492725 8_1:-71345051 None:intergenic
CTCTGAACAGTTTATGCAGA+TGG 0.494635 8_1:+71343488 Msa1211630:intron
TCGTAGAGTGCGGGAAGTTG+TGG 0.496064 8_1:+71345104 Msa1211630:CDS
ATCGCAGCAACATCATATAT+AGG 0.498248 8_1:-71343548 None:intergenic
AATCCAAATCCTGCCTTCAC+AGG 0.500896 8_1:-71345176 None:intergenic
ATCAATCAAACATTGAATGT+TGG 0.501793 8_1:-71345439 None:intergenic
TGTTGGCGGCCTGTGAAGGC+AGG 0.507951 8_1:+71345167 Msa1211630:three_prime_UTR
TGCTTGTTGGCGGCCTGTGA+AGG 0.512645 8_1:+71345163 Msa1211630:three_prime_UTR
AGAAAGAAATGATATAAGAG+TGG 0.517362 8_1:+71341285 Msa1211630:five_prime_UTR
CCATCCCATGGAGTATCATC+AGG 0.520795 8_1:-71342148 None:intergenic
ATCGAATCGAAAGCAAGGGC+GGG 0.525881 8_1:-71341384 None:intergenic
TGAATTTAGAATTTAAAGGC+AGG 0.525933 8_1:-71341352 None:intergenic
CTCATCAGTCTCTTCCTAGC+CGG 0.528410 8_1:-71341877 None:intergenic
TATATTACATGAACTGACAT+TGG 0.536734 8_1:-71345290 None:intergenic
AATGGATATTAGTTAAAATG+AGG 0.540225 8_1:+71345476 Msa1211630:three_prime_UTR
CAGAGTTGGACAGCTGACTA+AGG 0.541375 8_1:+71345129 Msa1211630:CDS
AAATGATATAAGAGTGGAAG+GGG 0.543391 8_1:+71341291 Msa1211630:five_prime_UTR
CATCTTGATATGCTTGTTGG+CGG 0.544398 8_1:+71345153 Msa1211630:three_prime_UTR
TATCGATGTCGACTCCGGCT+AGG 0.564364 8_1:+71341863 Msa1211630:exon
CTCTCTCCCAAACACACTAA+CGG 0.565652 8_1:+71341433 Msa1211630:five_prime_UTR
TGAGTACAATCGTAGAGTGC+GGG 0.565704 8_1:+71345095 Msa1211630:CDS
GCAGTCCTGATGATACTCCA+TGG 0.566145 8_1:+71342143 Msa1211630:intron
TTCTCCTGCAAACTCTGAAG+CGG 0.568993 8_1:+71345047 Msa1211630:CDS
TTGCAACATGACCCACCTGC+AGG 0.570976 8_1:+71341913 Msa1211630:CDS
GGAGCACCACTAATTCCTGC+AGG 0.573494 8_1:-71341928 None:intergenic
GGGAAGTTGTGGAGCAGAGT+TGG 0.573831 8_1:+71345115 Msa1211630:CDS
GAGGGATCGAATCGAAAGCA+AGG 0.576553 8_1:-71341389 None:intergenic
TGGCTTGTTCGGATAATCCT+CGG 0.581252 8_1:-71343356 None:intergenic
GTTTGCAGGAGAATTTGGGT+TGG 0.582593 8_1:-71345037 None:intergenic
GCACCACTAATTCCTGCAGG+TGG 0.583819 8_1:-71341925 None:intergenic
GAACAGTGTTGACAACAATC+CGG 0.587579 8_1:-71345331 None:intergenic
TCATAAGGATTATTTAAACC+CGG 0.588951 8_1:+71345389 Msa1211630:three_prime_UTR
CCTGATGATACTCCATGGGA+TGG 0.589928 8_1:+71342148 Msa1211630:CDS
GATCGAATCGAAAGCAAGGG+CGG 0.598847 8_1:-71341385 None:intergenic
TGGATATATTGCAAAATCAG+TGG 0.599561 8_1:+71343522 Msa1211630:CDS
GGTGGTATCGATGTCGACTC+CGG 0.602685 8_1:+71341858 Msa1211630:intron
CGGGAGACAAATCGTACAGT+GGG 0.603878 8_1:-71343379 None:intergenic
ATTTAAAGGCAGGCGTGGCG+TGG 0.605026 8_1:-71341342 None:intergenic
GAAATGATATAAGAGTGGAA+GGG 0.605544 8_1:+71341290 Msa1211630:five_prime_UTR
GATATAAGAGTGGAAGGGGA+CGG 0.607386 8_1:+71341295 Msa1211630:five_prime_UTR
AAAATTGTATCAATGTGCAC+CGG 0.608962 8_1:+71345312 Msa1211630:three_prime_UTR
ACTGACTCACCTCCATCCCA+TGG 0.613625 8_1:-71342160 None:intergenic
TTAGAATTTAAAGGCAGGCG+TGG 0.614403 8_1:-71341347 None:intergenic
GAATCGAAAGCAAGGGCGGG+CGG 0.632815 8_1:-71341381 None:intergenic
GAGACAAATCGTACAGTGGG+TGG 0.633963 8_1:-71343376 None:intergenic
CACCACTAATTCCTGCAGGT+GGG 0.640442 8_1:-71341924 None:intergenic
AGGATAACAATATCATGCTG+TGG 0.645864 8_1:+71341953 Msa1211630:CDS
CAGTCCTGATGATACTCCAT+GGG 0.646431 8_1:+71342144 Msa1211630:intron
AGGGATCGAATCGAAAGCAA+GGG 0.652260 8_1:-71341388 None:intergenic
GATGATACTCCATGGGATGG+AGG 0.662516 8_1:+71342151 Msa1211630:CDS
GTGAGTACAATCGTAGAGTG+CGG 0.663013 8_1:+71345094 Msa1211630:CDS
TGAACCTAACATTCATAACA+AGG 0.667261 8_1:-71345233 None:intergenic
AGCATGATATTGTTATCCTG+AGG 0.673403 8_1:-71341949 None:intergenic
TCGGGAGACAAATCGTACAG+TGG 0.690605 8_1:-71343380 None:intergenic
AGTGCAAACAGAATTCAACC+GGG 0.747050 8_1:-71345407 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATATTGAAAAACAA+AGG - chr8_1:71343037-71343056 None:intergenic 10.0%
!! TCCAAATATAATAATATAAT+GGG - chr8_1:71344878-71344897 None:intergenic 10.0%
!! TTCCAAATATAATAATATAA+TGG - chr8_1:71344879-71344898 None:intergenic 10.0%
!! TGATGAATACAAATAATATA+GGG - chr8_1:71341556-71341575 None:intergenic 15.0%
!! TTGATGAATACAAATAATAT+AGG - chr8_1:71341557-71341576 None:intergenic 15.0%
!!! GAATATTTTTTTTTTTGTCA+AGG + chr8_1:71342477-71342496 Msa1211630:intron 15.0%
!!! AGTTTTTTAAAATCTAAAAC+TGG + chr8_1:71342970-71342989 Msa1211630:intron 15.0%
!! TTAGATTAATGAAAACTAAA+TGG + chr8_1:71343865-71343884 Msa1211630:intron 15.0%
!!! ATATAATAATATAATGGGAT+GGG - chr8_1:71344873-71344892 None:intergenic 15.0%
!!! AATATAATAATATAATGGGA+TGG - chr8_1:71344874-71344893 None:intergenic 15.0%
!! TCCCATTATATTATTATATT+TGG + chr8_1:71344874-71344893 Msa1211630:intron 15.0%
!!! CTAATAAAATCATTGAATTT+TGG + chr8_1:71345517-71345536 Msa1211630:three_prime_UTR 15.0%
!!! ATGGTGAATTTAGAATTTAA+AGG - chr8_1:71341359-71341378 None:intergenic 20.0%
!! GCAATAAAATTGATTGAAAA+CGG - chr8_1:71341479-71341498 None:intergenic 20.0%
!!! TCCACTTTTTCTTAATTTTT+GGG + chr8_1:71341594-71341613 Msa1211630:intron 20.0%
!!! ACTTGTCATTAAATTGATTT+TGG + chr8_1:71341687-71341706 Msa1211630:intron 20.0%
!!! ACTTTGTTTAAGTATATGAA+TGG + chr8_1:71342083-71342102 Msa1211630:intron 20.0%
!!! TCTTTGCTCTTAATTTTTTT+AGG + chr8_1:71342818-71342837 Msa1211630:intron 20.0%
!!! GTATATATTTTGAGTTATTC+AGG + chr8_1:71343307-71343326 Msa1211630:intron 20.0%
!! TTGTTCTGACTTATAAATTA+TGG - chr8_1:71343778-71343797 None:intergenic 20.0%
!! AAATAAGTATGCTAAACAAA+GGG - chr8_1:71344158-71344177 None:intergenic 20.0%
!!! TTAATTCTCATTTTTCAAAG+AGG - chr8_1:71344525-71344544 None:intergenic 20.0%
!! TATCATTTGATATTTCACAT+AGG - chr8_1:71344632-71344651 None:intergenic 20.0%
!! TTATTATATTTGGAAGTTTC+CGG + chr8_1:71344884-71344903 Msa1211630:intron 20.0%
!! AAGTTTGATTCTATTTAGTA+TGG - chr8_1:71344923-71344942 None:intergenic 20.0%
!!! TGATTTTTGTTATGTATGAA+TGG + chr8_1:71345458-71345477 Msa1211630:three_prime_UTR 20.0%
!! AATGGATATTAGTTAAAATG+AGG + chr8_1:71345476-71345495 Msa1211630:three_prime_UTR 20.0%
! AGAAAGAAATGATATAAGAG+TGG + chr8_1:71341285-71341304 Msa1211630:five_prime_UTR 25.0%
!! TGAATTTAGAATTTAAAGGC+AGG - chr8_1:71341355-71341374 None:intergenic 25.0%
!!! CTCCACTTTTTCTTAATTTT+TGG + chr8_1:71341593-71341612 Msa1211630:intron 25.0%
!!! CCACTTTTTCTTAATTTTTG+GGG + chr8_1:71341595-71341614 Msa1211630:intron 25.0%
!!! TGTCATTAAATTGATTTTGG+AGG + chr8_1:71341690-71341709 Msa1211630:intron 25.0%
!!! TGGAAAAAATTGATTTTTGC+AGG + chr8_1:71341837-71341856 Msa1211630:intron 25.0%
!!! AAAAAATTGATTTTTGCAGG+TGG + chr8_1:71341840-71341859 Msa1211630:intron 25.0%
!! TTTTTATACTAGTGTCAGTT+AGG + chr8_1:71342221-71342240 Msa1211630:intron 25.0%
! AATACTTGTAACACACAAAA+TGG - chr8_1:71342255-71342274 None:intergenic 25.0%
! TTGAATAGTAAAGTCCAATT+TGG + chr8_1:71342300-71342319 Msa1211630:intron 25.0%
! AATAAGTTGTTTATCCAAAC+AGG - chr8_1:71342511-71342530 None:intergenic 25.0%
!! AAAATCTTTTATAGGATGGA+AGG - chr8_1:71342911-71342930 None:intergenic 25.0%
!! TTCCATCCTATAAAAGATTT+TGG + chr8_1:71342910-71342929 Msa1211630:intron 25.0%
!! TCCATCCTATAAAAGATTTT+GGG + chr8_1:71342911-71342930 Msa1211630:intron 25.0%
!!! TACAACATGTTTTGATTTGA+TGG - chr8_1:71343176-71343195 None:intergenic 25.0%
! ATCAAAACATGTTGTACATT+TGG + chr8_1:71343180-71343199 Msa1211630:intron 25.0%
! TCAAAACATGTTGTACATTT+GGG + chr8_1:71343181-71343200 Msa1211630:intron 25.0%
! TATAATCAATGAGATACACA+TGG + chr8_1:71343808-71343827 Msa1211630:intron 25.0%
! GAAATAAGTATGCTAAACAA+AGG - chr8_1:71344159-71344178 None:intergenic 25.0%
! AAAACTACAAAAAGCAATAC+AGG - chr8_1:71344294-71344313 None:intergenic 25.0%
! GAAAGAGAAATTTACAAAAC+AGG - chr8_1:71344334-71344353 None:intergenic 25.0%
!!! AATTTTAAGACTGTTTTGGA+GGG + chr8_1:71344465-71344484 Msa1211630:intron 25.0%
! AATTGCAGGTTATTGAAAAA+TGG - chr8_1:71344766-71344785 None:intergenic 25.0%
!! AGATGCAATAATTTTCTTTG+TGG + chr8_1:71344807-71344826 Msa1211630:intron 25.0%
! TATTATATTTGGAAGTTTCC+GGG + chr8_1:71344885-71344904 Msa1211630:intron 25.0%
! TTCATCTTATCTTTCATTCA+TGG + chr8_1:71345252-71345271 Msa1211630:three_prime_UTR 25.0%
! TATATTACATGAACTGACAT+TGG - chr8_1:71345293-71345312 None:intergenic 25.0%
! TCATAAGGATTATTTAAACC+CGG + chr8_1:71345389-71345408 Msa1211630:three_prime_UTR 25.0%
! ATCAATCAAACATTGAATGT+TGG - chr8_1:71345442-71345461 None:intergenic 25.0%
! GTTAAAATGAGGAATAAATC+TGG + chr8_1:71345487-71345506 Msa1211630:three_prime_UTR 25.0%
AGAAATGATATAAGAGTGGA+AGG + chr8_1:71341289-71341308 Msa1211630:five_prime_UTR 30.0%
GAAATGATATAAGAGTGGAA+GGG + chr8_1:71341290-71341309 Msa1211630:five_prime_UTR 30.0%
AAATGATATAAGAGTGGAAG+GGG + chr8_1:71341291-71341310 Msa1211630:five_prime_UTR 30.0%
AAATTGATTGAAAACGGAGA+GGG - chr8_1:71341473-71341492 None:intergenic 30.0%
AAAATTGATTGAAAACGGAG+AGG - chr8_1:71341474-71341493 None:intergenic 30.0%
CCCCAAAAATTAAGAAAAAG+TGG - chr8_1:71341598-71341617 None:intergenic 30.0%
AATCTCACAGTGAATTATGT+CGG - chr8_1:71341636-71341655 None:intergenic 30.0%
! TGTCTTGTGGAACTTTTAAT+TGG + chr8_1:71341817-71341836 Msa1211630:intron 30.0%
TGTGGAATGCTGTTATATTT+GGG + chr8_1:71341971-71341990 Msa1211630:CDS 30.0%
! TAGTTTGCAAATAAGAGTTG+TGG - chr8_1:71342195-71342214 None:intergenic 30.0%
!! ATTTTGTCTGAGACTGAATT+GGG - chr8_1:71342347-71342366 None:intergenic 30.0%
!! TATTTTGTCTGAGACTGAAT+TGG - chr8_1:71342348-71342367 None:intergenic 30.0%
! AGTTCCTCGCATTTTATATA+GGG + chr8_1:71342381-71342400 Msa1211630:intron 30.0%
! TAACCCTCAGATTTTCATAT+GGG - chr8_1:71342453-71342472 None:intergenic 30.0%
! TTAACCCTCAGATTTTCATA+TGG - chr8_1:71342454-71342473 None:intergenic 30.0%
!!! TTTTTTTTGTCAAGGACTGA+AGG + chr8_1:71342485-71342504 Msa1211630:intron 30.0%
!! TAAGTTTTGTGAGACTGTTT+GGG - chr8_1:71342730-71342749 None:intergenic 30.0%
!! ATAAGTTTTGTGAGACTGTT+TGG - chr8_1:71342731-71342750 None:intergenic 30.0%
TTAAGAGCAAAGACTCAATT+TGG - chr8_1:71342811-71342830 None:intergenic 30.0%
ATGTTAATATTAGCCGAACT+TGG + chr8_1:71342843-71342862 Msa1211630:intron 30.0%
TATGTTGCAAAGAACATCAT+AGG + chr8_1:71342885-71342904 Msa1211630:intron 30.0%
! ACCCAAAATCTTTTATAGGA+TGG - chr8_1:71342915-71342934 None:intergenic 30.0%
! AGCAACCCAAAATCTTTTAT+AGG - chr8_1:71342919-71342938 None:intergenic 30.0%
CAAAACATGTTGTACATTTG+GGG + chr8_1:71343182-71343201 Msa1211630:intron 30.0%
AAAACATGTTGTACATTTGG+GGG + chr8_1:71343183-71343202 Msa1211630:intron 30.0%
TACTGTTTGGATGAAACATT+CGG - chr8_1:71343402-71343421 None:intergenic 30.0%
TCATCCAAACAGTAAGTATT+AGG + chr8_1:71343408-71343427 Msa1211630:intron 30.0%
TGGATATATTGCAAAATCAG+TGG + chr8_1:71343522-71343541 Msa1211630:CDS 30.0%
ACACTATACTAAATGATACC+TGG - chr8_1:71343586-71343605 None:intergenic 30.0%
! ATCTCATTGATTATATGCTC+TGG - chr8_1:71343803-71343822 None:intergenic 30.0%
ACTAAATGGAAATGCCAAAT+AGG + chr8_1:71343879-71343898 Msa1211630:intron 30.0%
CTAAATGGAAATGCCAAATA+GGG + chr8_1:71343880-71343899 Msa1211630:intron 30.0%
AAGGGAATCATTTCAAAACA+GGG - chr8_1:71343941-71343960 None:intergenic 30.0%
AAAGGGAATCATTTCAAAAC+AGG - chr8_1:71343942-71343961 None:intergenic 30.0%
TTCTCATCAAAGAAATTGGA+AGG - chr8_1:71344009-71344028 None:intergenic 30.0%
CCAATTCTCATCAAAGAAAT+TGG - chr8_1:71344013-71344032 None:intergenic 30.0%
CCAATTTCTTTGATGAGAAT+TGG + chr8_1:71344010-71344029 Msa1211630:intron 30.0%
TTGAACTGTGCAATTCATAT+TGG + chr8_1:71344066-71344085 Msa1211630:intron 30.0%
TCTTAAGTCTGATATGCAAA+TGG + chr8_1:71344222-71344241 Msa1211630:intron 30.0%
AGAGAAATTTACAAAACAGG+AGG - chr8_1:71344331-71344350 None:intergenic 30.0%
TGTGGAAAACAAACATAACA+AGG - chr8_1:71344400-71344419 None:intergenic 30.0%
GAAAAAGACAGTAAACTTTG+TGG - chr8_1:71344418-71344437 None:intergenic 30.0%
! CAAAGTTTACTGTCTTTTTC+TGG + chr8_1:71344418-71344437 Msa1211630:intron 30.0%
!!! AGGGAATTTTAAGACTGTTT+TGG + chr8_1:71344461-71344480 Msa1211630:intron 30.0%
!!! GAATTTTAAGACTGTTTTGG+AGG + chr8_1:71344464-71344483 Msa1211630:intron 30.0%
!!! ATTTTAAGACTGTTTTGGAG+GGG + chr8_1:71344466-71344485 Msa1211630:intron 30.0%
TGAGAATTAAACCTGTTATG+AGG + chr8_1:71344535-71344554 Msa1211630:intron 30.0%
TGATATGCAAGTATTCAATG+TGG + chr8_1:71344712-71344731 Msa1211630:intron 30.0%
ATTATATTTGGAAGTTTCCG+GGG + chr8_1:71344886-71344905 Msa1211630:intron 30.0%
AGAAAATAATCACACAATGC+TGG - chr8_1:71344975-71344994 None:intergenic 30.0%
! CATTCCTTGTTATGAATGTT+AGG + chr8_1:71345229-71345248 Msa1211630:three_prime_UTR 30.0%
TGAACCTAACATTCATAACA+AGG - chr8_1:71345236-71345255 None:intergenic 30.0%
AAAATTGTATCAATGTGCAC+CGG + chr8_1:71345312-71345331 Msa1211630:three_prime_UTR 30.0%
TAAGTTGTACTTGTCTGAAT+TGG + chr8_1:71345360-71345379 Msa1211630:three_prime_UTR 30.0%
CTGAATTGGTTATGATCATA+AGG + chr8_1:71345374-71345393 Msa1211630:three_prime_UTR 30.0%
TCCAGAGTATAAATCGATCA+CGG - chr8_1:71341521-71341540 None:intergenic 35.0%
TTCAACGTAATTACATGTGC+CGG - chr8_1:71341731-71341750 None:intergenic 35.0%
AGCATGATATTGTTATCCTG+AGG - chr8_1:71341952-71341971 None:intergenic 35.0%
AGGATAACAATATCATGCTG+TGG + chr8_1:71341953-71341972 Msa1211630:CDS 35.0%
CTGTGGAATGCTGTTATATT+TGG + chr8_1:71341970-71341989 Msa1211630:CDS 35.0%
TTTGCAAATAAGAGTTGTGG+TGG - chr8_1:71342192-71342211 None:intergenic 35.0%
! CAGTTCCTCGCATTTTATAT+AGG + chr8_1:71342380-71342399 Msa1211630:intron 35.0%
TGTACCCTATATAAAATGCG+AGG - chr8_1:71342388-71342407 None:intergenic 35.0%
TCTCCCATATGAAAATCTGA+GGG + chr8_1:71342447-71342466 Msa1211630:intron 35.0%
TATAGCACAAGCGATTATCA+TGG + chr8_1:71342538-71342557 Msa1211630:intron 35.0%
TTAAATCAGCCAATCCAAAC+AGG + chr8_1:71342765-71342784 Msa1211630:intron 35.0%
! ATGCCATCTAAAACCAAGTT+CGG - chr8_1:71342859-71342878 None:intergenic 35.0%
GATAATCCTCGGAAAATTGA+AGG - chr8_1:71343348-71343367 None:intergenic 35.0%
ACTGTTTGGATGAAACATTC+GGG - chr8_1:71343401-71343420 None:intergenic 35.0%
TCCAAACAGTAAGTATTAGG+TGG + chr8_1:71343411-71343430 Msa1211630:intron 35.0%
TCCACCTAATACTTACTGTT+TGG - chr8_1:71343415-71343434 None:intergenic 35.0%
GACACAACTAACAGATAAGA+AGG - chr8_1:71343447-71343466 None:intergenic 35.0%
AGAGCAGCAATTACATTAGT+TGG - chr8_1:71343472-71343491 None:intergenic 35.0%
ATCGCAGCAACATCATATAT+AGG - chr8_1:71343551-71343570 None:intergenic 35.0%
TATGAATGACTCTGTGATGA+AGG + chr8_1:71343835-71343854 Msa1211630:intron 35.0%
AAATGCCAAATAGGGTGAAT+GGG + chr8_1:71343888-71343907 Msa1211630:intron 35.0%
AGGGAATCATTTCAAAACAG+GGG - chr8_1:71343940-71343959 None:intergenic 35.0%
TTAAACTGTTCAGTGCAAAG+GGG - chr8_1:71343976-71343995 None:intergenic 35.0%
CTTAAACTGTTCAGTGCAAA+GGG - chr8_1:71343977-71343996 None:intergenic 35.0%
ACTTAAACTGTTCAGTGCAA+AGG - chr8_1:71343978-71343997 None:intergenic 35.0%
GGCAAATCAACAAATAGCTA+TGG - chr8_1:71344137-71344156 None:intergenic 35.0%
TTCTGTAGTTTCTTTGTTCG+TGG + chr8_1:71344176-71344195 Msa1211630:intron 35.0%
AAGCAATACAGGAGAAAGAA+GGG - chr8_1:71344283-71344302 None:intergenic 35.0%
AAAGCAATACAGGAGAAAGA+AGG - chr8_1:71344284-71344303 None:intergenic 35.0%
GCTTGCAACCTAAAAATCTA+AGG + chr8_1:71344369-71344388 Msa1211630:intron 35.0%
! AGGCTCTTCCTTAGATTTTT+AGG - chr8_1:71344380-71344399 None:intergenic 35.0%
ATTCCCTAGTAAACAGAAGT+GGG - chr8_1:71344448-71344467 None:intergenic 35.0%
AATTCCCTAGTAAACAGAAG+TGG - chr8_1:71344449-71344468 None:intergenic 35.0%
ACTGTAATATCTGCCAATGA+TGG - chr8_1:71344598-71344617 None:intergenic 35.0%
! GCTTTAATGTTTTCCTGTGT+GGG + chr8_1:71344655-71344674 Msa1211630:intron 35.0%
ATATCACACTCTTGCATCAA+GGG - chr8_1:71344698-71344717 None:intergenic 35.0%
AATGCTGGTCAATACATAAC+AGG - chr8_1:71344960-71344979 None:intergenic 35.0%
CAAAGCAATGACTGTCAAAA+AGG - chr8_1:71345012-71345031 None:intergenic 35.0%
TTAGAATTTAAAGGCAGGCG+TGG - chr8_1:71341350-71341369 None:intergenic 40.0%
AATCGATCACGGAAACAACA+AGG - chr8_1:71341510-71341529 None:intergenic 40.0%
! TCCGTGATCGATTTATACTC+TGG + chr8_1:71341517-71341536 Msa1211630:intron 40.0%
TCTGTGCTTCATAGCATACT+AGG + chr8_1:71341791-71341810 Msa1211630:intron 40.0%
!! GCATACTAGGTTTTGTCTTG+TGG + chr8_1:71341804-71341823 Msa1211630:intron 40.0%
GCTGTTATATTTGGGTGAGT+TGG + chr8_1:71341979-71341998 Msa1211630:intron 40.0%
ATTGGGCCGTAAGAAAAATG+CGG - chr8_1:71342330-71342349 None:intergenic 40.0%
TCCCTCTATCATTATAGCAG+AGG + chr8_1:71342414-71342433 Msa1211630:intron 40.0%
CCCTCTGCTATAATGATAGA+GGG - chr8_1:71342418-71342437 None:intergenic 40.0%
CCCTCTATCATTATAGCAGA+GGG + chr8_1:71342415-71342434 Msa1211630:intron 40.0%
TCCCTCTGCTATAATGATAG+AGG - chr8_1:71342419-71342438 None:intergenic 40.0%
GTCTCCCATATGAAAATCTG+AGG + chr8_1:71342446-71342465 Msa1211630:intron 40.0%
! TTGGTCCCTGACAATTTTCT+CGG - chr8_1:71342792-71342811 None:intergenic 40.0%
!!! TAGCCGAACTTGGTTTTAGA+TGG + chr8_1:71342853-71342872 Msa1211630:intron 40.0%
!!! GATTTTGGGTTGCTCTAGAT+AGG + chr8_1:71342925-71342944 Msa1211630:intron 40.0%
! TAAAACTGGCTTCTGCTTTG+TGG + chr8_1:71342984-71343003 Msa1211630:intron 40.0%
CTCTGAACAGTTTATGCAGA+TGG + chr8_1:71343488-71343507 Msa1211630:intron 40.0%
!! TGCAGATGGAAGTATTTGTC+TGG + chr8_1:71343502-71343521 Msa1211630:CDS 40.0%
GAAATGCCAAATAGGGTGAA+TGG + chr8_1:71343887-71343906 Msa1211630:intron 40.0%
AATGCCAAATAGGGTGAATG+GGG + chr8_1:71343889-71343908 Msa1211630:intron 40.0%
AAGGGGGGTTGAAGAAAAAA+GGG - chr8_1:71343959-71343978 None:intergenic 40.0%
AAAGGGGGGTTGAAGAAAAA+AGG - chr8_1:71343960-71343979 None:intergenic 40.0%
TAAACTGTTCAGTGCAAAGG+GGG - chr8_1:71343975-71343994 None:intergenic 40.0%
AGCAATACAGGAGAAAGAAG+GGG - chr8_1:71344282-71344301 None:intergenic 40.0%
TTCTCCCACTTCTGTTTACT+AGG + chr8_1:71344441-71344460 Msa1211630:intron 40.0%
TCTCCCACTTCTGTTTACTA+GGG + chr8_1:71344442-71344461 Msa1211630:intron 40.0%
! CGCTTTAATGTTTTCCTGTG+TGG + chr8_1:71344654-71344673 Msa1211630:intron 40.0%
CATATCACACTCTTGCATCA+AGG - chr8_1:71344699-71344718 None:intergenic 40.0%
TGTAGAAGCTGAGTAATTGC+AGG - chr8_1:71344780-71344799 None:intergenic 40.0%
CAATTACTCAGCTTCTACAC+TGG + chr8_1:71344781-71344800 Msa1211630:intron 40.0%
GTATGGAGATAAAAGTTCCC+CGG - chr8_1:71344906-71344925 None:intergenic 40.0%
! TTTGCAGGAGAATTTGGGTT+GGG - chr8_1:71345039-71345058 None:intergenic 40.0%
CAGAGTTTGCAGGAGAATTT+GGG - chr8_1:71345044-71345063 None:intergenic 40.0%
TCAGAGTTTGCAGGAGAATT+TGG - chr8_1:71345045-71345064 None:intergenic 40.0%
GGTCATCTTGATATGCTTGT+TGG + chr8_1:71345150-71345169 Msa1211630:three_prime_UTR 40.0%
! CATCTTGATATGCTTGTTGG+CGG + chr8_1:71345153-71345172 Msa1211630:three_prime_UTR 40.0%
TGAGTTTGCATGACTCTCAT+AGG - chr8_1:71345211-71345230 None:intergenic 40.0%
!! GAACAGTGTTGACAACAATC+CGG - chr8_1:71345334-71345353 None:intergenic 40.0%
AGTGCAAACAGAATTCAACC+GGG - chr8_1:71345410-71345429 None:intergenic 40.0%
CAGTGCAAACAGAATTCAAC+CGG - chr8_1:71345411-71345430 None:intergenic 40.0%
GATATAAGAGTGGAAGGGGA+CGG + chr8_1:71341295-71341314 Msa1211630:five_prime_UTR 45.0%
AGGGATCGAATCGAAAGCAA+GGG - chr8_1:71341391-71341410 None:intergenic 45.0%
CTCTCTCCCAAACACACTAA+CGG + chr8_1:71341433-71341452 Msa1211630:five_prime_UTR 45.0%
TCTCTCCCAAACACACTAAC+GGG + chr8_1:71341434-71341453 Msa1211630:five_prime_UTR 45.0%
ACTGACCCGTTAGTGTGTTT+GGG - chr8_1:71341442-71341461 None:intergenic 45.0%
! GAGGTTTTGCCTTTTGTTGC+CGG + chr8_1:71341709-71341728 Msa1211630:intron 45.0%
! TTACATGTGCCGGCAACAAA+AGG - chr8_1:71341721-71341740 None:intergenic 45.0%
CTCTGCTTTCTTGTCTGCTT+TGG + chr8_1:71342117-71342136 Msa1211630:intron 45.0%
CAGTCCTGATGATACTCCAT+GGG + chr8_1:71342144-71342163 Msa1211630:intron 45.0%
AAAAATGCGGGGGACCAAAT+TGG - chr8_1:71342317-71342336 None:intergenic 45.0%
TTGGGCCGTAAGAAAAATGC+GGG - chr8_1:71342329-71342348 None:intergenic 45.0%
! TTTGGGATGAAAACAGCGCA+TGG - chr8_1:71342713-71342732 None:intergenic 45.0%
! ACAATTTTCTCGGGCCTGTT+TGG - chr8_1:71342782-71342801 None:intergenic 45.0%
! TGGTCCCTGACAATTTTCTC+GGG - chr8_1:71342791-71342810 None:intergenic 45.0%
GTGCAAATGTAGCCTGTAAC+AGG - chr8_1:71343102-71343121 None:intergenic 45.0%
! GTTGAGCCTTCAATTTTCCG+AGG + chr8_1:71343339-71343358 Msa1211630:CDS 45.0%
!! TGGCTTGTTCGGATAATCCT+CGG - chr8_1:71343359-71343378 None:intergenic 45.0%
TGCGATTCTCACTTCTATCC+AGG + chr8_1:71343565-71343584 Msa1211630:CDS 45.0%
AAACTGTTCAGTGCAAAGGG+GGG - chr8_1:71343974-71343993 None:intergenic 45.0%
TTCTTTGTTCGTGGCTGCTA+AGG + chr8_1:71344185-71344204 Msa1211630:intron 45.0%
GCAATACAGGAGAAAGAAGG+GGG - chr8_1:71344281-71344300 None:intergenic 45.0%
!!! TGTTTTGGAGGGGTTGTGAA+GGG + chr8_1:71344476-71344495 Msa1211630:intron 45.0%
TGAAGGGTCAACTAGCTGAA+AGG + chr8_1:71344492-71344511 Msa1211630:intron 45.0%
!! TTTGGGAAGCACCTCATAAC+AGG - chr8_1:71344549-71344568 None:intergenic 45.0%
! TGTTATGAGGTGCTTCCCAA+AGG + chr8_1:71344548-71344567 Msa1211630:intron 45.0%
CTATCAGCACACAGTCCTTT+GGG - chr8_1:71344566-71344585 None:intergenic 45.0%
ACTATCAGCACACAGTCCTT+TGG - chr8_1:71344567-71344586 None:intergenic 45.0%
ATAGTCTGCAAGGCCATCAT+TGG + chr8_1:71344582-71344601 Msa1211630:intron 45.0%
! TGTTTTCCTGTGTGGGATCA+CGG + chr8_1:71344662-71344681 Msa1211630:intron 45.0%
GAGTCAGGAAGACGAAGATT+AGG - chr8_1:71344837-71344856 None:intergenic 45.0%
GGATGTTAAAGAGTGGAGTC+AGG - chr8_1:71344852-71344871 None:intergenic 45.0%
TGGGATGGGATGTTAAAGAG+TGG - chr8_1:71344859-71344878 None:intergenic 45.0%
GTTTGCAGGAGAATTTGGGT+TGG - chr8_1:71345040-71345059 None:intergenic 45.0%
! TTCTCCTGCAAACTCTGAAG+CGG + chr8_1:71345047-71345066 Msa1211630:CDS 45.0%
GTGAGTACAATCGTAGAGTG+CGG + chr8_1:71345094-71345113 Msa1211630:CDS 45.0%
TGAGTACAATCGTAGAGTGC+GGG + chr8_1:71345095-71345114 Msa1211630:CDS 45.0%
AATCCAAATCCTGCCTTCAC+AGG - chr8_1:71345179-71345198 None:intergenic 45.0%
ATCGAATCGAAAGCAAGGGC+GGG - chr8_1:71341387-71341406 None:intergenic 50.0%
GATCGAATCGAAAGCAAGGG+CGG - chr8_1:71341388-71341407 None:intergenic 50.0%
GAGGGATCGAATCGAAAGCA+AGG - chr8_1:71341392-71341411 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG - chr8_1:71341418-71341437 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr8_1:71341417-71341436 None:intergenic 50.0%
GACTGACCCGTTAGTGTGTT+TGG - chr8_1:71341443-71341462 None:intergenic 50.0%
CTCATCAGTCTCTTCCTAGC+CGG - chr8_1:71341880-71341899 None:intergenic 50.0%
CACCACTAATTCCTGCAGGT+GGG - chr8_1:71341927-71341946 None:intergenic 50.0%
AGGAATTAGTGGTGCTCCTC+AGG + chr8_1:71341933-71341952 Msa1211630:CDS 50.0%
GCAGTCCTGATGATACTCCA+TGG + chr8_1:71342143-71342162 Msa1211630:intron 50.0%
! CCATCCCATGGAGTATCATC+AGG - chr8_1:71342151-71342170 None:intergenic 50.0%
CCTGATGATACTCCATGGGA+TGG + chr8_1:71342148-71342167 Msa1211630:CDS 50.0%
GATGATACTCCATGGGATGG+AGG + chr8_1:71342151-71342170 Msa1211630:CDS 50.0%
! GGTCCCCCGCATTTTTCTTA+CGG + chr8_1:71342321-71342340 Msa1211630:intron 50.0%
TGGGCCGTAAGAAAAATGCG+GGG - chr8_1:71342328-71342347 None:intergenic 50.0%
TCAAGGACTGAAGGCCTGTT+TGG + chr8_1:71342494-71342513 Msa1211630:intron 50.0%
TTTCTCGGGCCTGTTTGGAT+TGG - chr8_1:71342777-71342796 None:intergenic 50.0%
ACAGGCCCGAGAAAATTGTC+AGG + chr8_1:71342783-71342802 Msa1211630:intron 50.0%
CAGGCCCGAGAAAATTGTCA+GGG + chr8_1:71342784-71342803 Msa1211630:intron 50.0%
GAGACAAATCGTACAGTGGG+TGG - chr8_1:71343379-71343398 None:intergenic 50.0%
CGGGAGACAAATCGTACAGT+GGG - chr8_1:71343382-71343401 None:intergenic 50.0%
TCGGGAGACAAATCGTACAG+TGG - chr8_1:71343383-71343402 None:intergenic 50.0%
CTCACCCCATTCACCCTATT+TGG - chr8_1:71343896-71343915 None:intergenic 50.0%
!!! CTGTTTTGGAGGGGTTGTGA+AGG + chr8_1:71344475-71344494 Msa1211630:intron 50.0%
! CTGTGTGCTGATAGTCTGCA+AGG + chr8_1:71344572-71344591 Msa1211630:intron 50.0%
! CTCTTGCATCAAGGGCACAT+TGG - chr8_1:71344690-71344709 None:intergenic 50.0%
CAGAGTTGGACAGCTGACTA+AGG + chr8_1:71345129-71345148 Msa1211630:CDS 50.0%
!! GGCGTGGTTGGTTTCGATTC+TGG - chr8_1:71341329-71341348 None:intergenic 55.0%
ATTTAAAGGCAGGCGTGGCG+TGG - chr8_1:71341345-71341364 None:intergenic 55.0%
GAGAGAGAGAGAGGGAGAGA+GGG - chr8_1:71341410-71341429 None:intergenic 55.0%
AGAGAGAGAGAGAGGGAGAG+AGG - chr8_1:71341411-71341430 None:intergenic 55.0%
!! GGTGGTATCGATGTCGACTC+CGG + chr8_1:71341858-71341877 Msa1211630:intron 55.0%
!! TATCGATGTCGACTCCGGCT+AGG + chr8_1:71341863-71341882 Msa1211630:exon 55.0%
TTGCAACATGACCCACCTGC+AGG + chr8_1:71341913-71341932 Msa1211630:CDS 55.0%
GACCCACCTGCAGGAATTAG+TGG + chr8_1:71341922-71341941 Msa1211630:CDS 55.0%
GCACCACTAATTCCTGCAGG+TGG - chr8_1:71341928-71341947 None:intergenic 55.0%
! GGAGCACCACTAATTCCTGC+AGG - chr8_1:71341931-71341950 None:intergenic 55.0%
! ACTGACTCACCTCCATCCCA+TGG - chr8_1:71342163-71342182 None:intergenic 55.0%
GGGCCGTAAGAAAAATGCGG+GGG - chr8_1:71342327-71342346 None:intergenic 55.0%
CTACCTGATGCGCCTGTTAC+AGG + chr8_1:71343087-71343106 Msa1211630:intron 55.0%
TAGCCTGTAACAGGCGCATC+AGG - chr8_1:71343093-71343112 None:intergenic 55.0%
!! CGTACAGTGGGTGGCTTGTT+CGG - chr8_1:71343370-71343389 None:intergenic 55.0%
TTGGAACCGTGATCCCACAC+AGG - chr8_1:71344671-71344690 None:intergenic 55.0%
TCGTAGAGTGCGGGAAGTTG+TGG + chr8_1:71345104-71345123 Msa1211630:CDS 55.0%
GGGAAGTTGTGGAGCAGAGT+TGG + chr8_1:71345115-71345134 Msa1211630:CDS 55.0%
GAATCGAAAGCAAGGGCGGG+CGG - chr8_1:71341384-71341403 None:intergenic 60.0%
CGAGCCGCTTCAGAGTTTGC+AGG - chr8_1:71345054-71345073 None:intergenic 60.0%
! TGCTTGTTGGCGGCCTGTGA+AGG + chr8_1:71345163-71345182 Msa1211630:three_prime_UTR 60.0%
CGGCCTGTGAAGGCAGGATT+TGG + chr8_1:71345173-71345192 Msa1211630:three_prime_UTR 60.0%
AAAGGCAGGCGTGGCGTGGT+TGG - chr8_1:71341341-71341360 None:intergenic 65.0%
AAAGCAAGGGCGGGCGGCTA+TGG - chr8_1:71341378-71341397 None:intergenic 65.0%
TGTTGGCGGCCTGTGAAGGC+AGG + chr8_1:71345167-71345186 Msa1211630:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71341268 71345544 71341268 ID=Msa1211630;Name=Msa1211630
chr8_1 mRNA 71341268 71345544 71341268 ID=Msa1211630-mRNA-1;Parent=Msa1211630;Name=Msa1211630-mRNA-1;_AED=0.09;_eAED=0.09;_QI=197|1|1|1|0.8|0.66|6|395|152
chr8_1 exon 71341268 71341455 71341268 ID=Msa1211630-mRNA-1:exon:23920;Parent=Msa1211630-mRNA-1
chr8_1 exon 71341859 71341992 71341859 ID=Msa1211630-mRNA-1:exon:23921;Parent=Msa1211630-mRNA-1
chr8_1 exon 71342147 71342172 71342147 ID=Msa1211630-mRNA-1:exon:23922;Parent=Msa1211630-mRNA-1
chr8_1 exon 71343329 71343418 71343329 ID=Msa1211630-mRNA-1:exon:23923;Parent=Msa1211630-mRNA-1
chr8_1 exon 71343498 71343586 71343498 ID=Msa1211630-mRNA-1:exon:23924;Parent=Msa1211630-mRNA-1
chr8_1 exon 71345021 71345544 71345021 ID=Msa1211630-mRNA-1:exon:23925;Parent=Msa1211630-mRNA-1
chr8_1 five_prime_UTR 71341268 71341455 71341268 ID=Msa1211630-mRNA-1:five_prime_utr;Parent=Msa1211630-mRNA-1
chr8_1 five_prime_UTR 71341859 71341867 71341859 ID=Msa1211630-mRNA-1:five_prime_utr;Parent=Msa1211630-mRNA-1
chr8_1 CDS 71341868 71341992 71341868 ID=Msa1211630-mRNA-1:cds;Parent=Msa1211630-mRNA-1
chr8_1 CDS 71342147 71342172 71342147 ID=Msa1211630-mRNA-1:cds;Parent=Msa1211630-mRNA-1
chr8_1 CDS 71343329 71343418 71343329 ID=Msa1211630-mRNA-1:cds;Parent=Msa1211630-mRNA-1
chr8_1 CDS 71343498 71343586 71343498 ID=Msa1211630-mRNA-1:cds;Parent=Msa1211630-mRNA-1
chr8_1 CDS 71345021 71345149 71345021 ID=Msa1211630-mRNA-1:cds;Parent=Msa1211630-mRNA-1
chr8_1 three_prime_UTR 71345150 71345544 71345150 ID=Msa1211630-mRNA-1:three_prime_utr;Parent=Msa1211630-mRNA-1
Gene Sequence

>Msa1211630

ATGTCGACTCCGGCTAGGAAGAGACTGATGAGAGATTTCAAGAGATTGCAACATGACCCACCTGCAGGAATTAGTGGTGCTCCTCAGGATAACAATATCATGCTGTGGAATGCTGTTATATTTGGTCCTGATGATACTCCATGGGATGGAGGCACGTTTAAGTTGAGCCTTCAATTTTCCGAGGATTATCCGAACAAGCCACCCACTGTACGATTTGTCTCCCGAATGTTTCATCCAAACATTTATGCAGATGGAAGTATTTGTCTGGATATATTGCAAAATCAGTGGAGTCCTATATATGATGTTGCTGCGATTCTCACTTCTATCCAGTCATTGCTTTGTGATCCCAACCCAAATTCTCCTGCAAACTCTGAAGCGGCTCGAATGTTCAGTGAGAACAAGCGTGAGTACAATCGTAGAGTGCGGGAAGTTGTGGAGCAGAGTTGGACAGCTGACTAA
Protein sequence

>Msa1211630

MSTPARKRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYNRRVREVVEQSWTAD*