AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1299490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299490 MtrunA17_Chr4g0060351 100.000 152 0 0 1 152 1 152 1.40e-113 318
Msa1299490 MtrunA17_Chr8g0362321 97.368 152 4 0 1 152 1 152 1.53e-111 313
Msa1299490 MtrunA17_Chr5g0432831 45.890 146 74 1 5 150 2 142 2.67e-45 145
Msa1299490 MtrunA17_Chr3g0108261 45.890 146 74 1 5 150 2 142 3.12e-45 145
Msa1299490 MtrunA17_Chr7g0275361 45.205 146 75 1 5 150 2 142 7.54e-45 144
Msa1299490 MtrunA17_Chr1g0154011 45.890 146 74 1 5 150 2 142 1.11e-44 143
Msa1299490 MtrunA17_Chr8g0369311 46.575 146 73 1 5 150 58 198 3.06e-44 144
Msa1299490 MtrunA17_Chr4g0037011 44.521 146 76 1 5 150 2 142 1.77e-43 140
Msa1299490 MtrunA17_Chr4g0038891 46.853 143 75 1 2 144 30 171 1.33e-40 134
Msa1299490 MtrunA17_Chr5g0396451 45.652 138 74 1 7 144 35 171 9.55e-39 129
Msa1299490 MtrunA17_Chr2g0316201 40.146 137 82 0 7 143 8 144 2.46e-38 127
Msa1299490 MtrunA17_Chr4g0053191 42.208 154 82 2 1 147 1 154 6.16e-38 127
Msa1299490 MtrunA17_Chr5g0401831 41.558 154 83 2 1 147 1 154 1.62e-37 125
Msa1299490 MtrunA17_Chr2g0316211 41.085 129 76 0 8 136 9 137 3.51e-37 124
Msa1299490 MtrunA17_Chr4g0031181 39.416 137 70 1 25 148 26 162 2.08e-34 118
Msa1299490 MtrunA17_Chr3g0139901 38.732 142 83 3 5 142 4 145 1.27e-33 115
Msa1299490 MtrunA17_Chr1g0199951 41.958 143 81 2 2 143 39 180 9.35e-33 114
Msa1299490 MtrunA17_Chr2g0328001 36.496 137 74 1 25 148 26 162 1.37e-30 108
Msa1299490 MtrunA17_Chr7g0254701 36.029 136 87 0 7 142 13 148 1.93e-29 108
Msa1299490 MtrunA17_Chr8g0378601 34.848 132 86 0 7 138 25 156 2.41e-28 102
Msa1299490 MtrunA17_Chr3g0131871 34.507 142 84 2 10 142 1 142 9.87e-27 99.4
Msa1299490 MtrunA17_Chr8g0348701 30.201 149 98 3 1 147 1 145 2.19e-25 95.5
Msa1299490 MtrunA17_Chr4g0059291 29.530 149 99 3 1 147 1 145 5.63e-25 94.7
Msa1299490 MtrunA17_Chr8g0387131 32.000 150 94 4 1 147 1 145 2.87e-23 90.1
Msa1299490 MtrunA17_Chr5g0403591 27.778 144 103 1 8 151 33 175 4.34e-21 84.3
Msa1299490 MtrunA17_Chr8g0376111 32.857 140 82 4 7 134 43 182 1.14e-17 77.8
Msa1299490 MtrunA17_Chr4g0045101 33.784 148 85 5 7 141 37 184 2.04e-17 77.0
Msa1299490 MtrunA17_Chr7g0233611 33.571 140 81 4 7 134 38 177 5.99e-17 75.9
Msa1299490 MtrunA17_Chr8g0372391 36.792 106 65 2 1 105 12 116 8.46e-17 72.8
Msa1299490 MtrunA17_Chr7g0233631 31.410 156 89 5 7 150 38 187 1.55e-16 74.7
Msa1299490 MtrunA17_Chr8g0345331 31.092 119 73 3 7 120 8 122 1.01e-15 71.6
Msa1299490 MtrunA17_Chr5g0404931 35.849 106 66 2 1 105 12 116 1.09e-15 69.7
Msa1299490 MtrunA17_Chr1g0186291 26.667 105 63 2 46 149 30 121 1.57e-12 61.2
Msa1299490 MtrunA17_Chr1g0198021 31.973 147 86 6 7 139 138 284 3.14e-12 62.8
Msa1299490 MtrunA17_Chr4g0068301 29.457 129 81 4 1 124 7 130 4.05e-12 62.4
Msa1299490 MtrunA17_Chr2g0317631 31.148 122 74 4 7 118 307 428 9.34e-12 61.6
Msa1299490 MtrunA17_Chr8g0376201 30.137 146 88 5 7 138 193 338 2.68e-11 60.5
Msa1299490 MtrunA17_Chr1g0211131 30.081 123 74 4 7 118 823 944 4.33e-11 60.1
Msa1299490 MtrunA17_Chr2g0282211 29.268 123 75 3 7 118 682 803 5.12e-11 59.7
Msa1299490 MtrunA17_Chr7g0222941 28.689 122 77 3 7 118 91 212 9.40e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1299490 AT2G02760.1 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1299490 AT2G02760.2 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1299490 AT2G02760.4 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1299490 AT2G02760.3 98.684 152 2 0 1 152 1 152 1.18e-111 313
Msa1299490 AT1G14400.1 97.368 152 4 0 1 152 1 152 1.69e-110 310
Msa1299490 AT1G14400.2 97.368 152 4 0 1 152 1 152 1.69e-110 310
Msa1299490 AT5G62540.1 86.000 150 21 0 1 150 1 150 1.96e-98 280
Msa1299490 AT4G27960.1 45.205 146 75 1 5 150 2 142 4.64e-45 145
Msa1299490 AT1G64230.2 44.521 146 76 1 5 150 2 142 1.15e-44 144
Msa1299490 AT1G64230.1 44.521 146 76 1 5 150 2 142 1.15e-44 144
Msa1299490 AT5G53300.2 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1299490 AT5G53300.4 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1299490 AT5G53300.1 45.205 146 75 1 5 150 2 142 1.30e-44 144
Msa1299490 AT4G27960.2 45.205 146 75 1 5 150 32 172 3.41e-44 144
Msa1299490 AT3G08690.2 44.521 146 76 1 5 150 2 142 5.24e-44 142
Msa1299490 AT3G08690.1 44.521 146 76 1 5 150 2 142 5.24e-44 142
Msa1299490 AT2G16740.1 43.151 146 78 1 5 150 2 142 7.27e-44 142
Msa1299490 AT5G41700.5 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1299490 AT5G41700.2 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1299490 AT5G41700.1 43.836 146 77 1 5 150 2 142 1.12e-43 141
Msa1299490 AT3G08700.2 46.259 147 73 2 5 150 2 143 3.56e-43 140
Msa1299490 AT3G08700.1 46.259 147 73 2 5 150 2 143 3.56e-43 140
Msa1299490 AT5G56150.1 44.521 146 76 1 5 150 2 142 6.44e-43 139
Msa1299490 AT1G64230.3 43.226 155 74 3 5 150 2 151 1.13e-41 136
Msa1299490 AT5G41700.4 43.537 147 77 2 5 150 2 143 1.33e-41 136
Msa1299490 AT3G20060.1 46.429 140 74 1 7 146 39 177 2.80e-39 131
Msa1299490 AT1G50490.1 47.143 140 73 1 7 146 38 176 2.08e-38 129
Msa1299490 AT1G16890.2 40.146 137 82 0 7 143 8 144 4.09e-38 127
Msa1299490 AT3G20060.2 49.194 124 62 1 23 146 10 132 6.47e-38 126
Msa1299490 AT1G78870.2 40.146 137 82 0 7 143 8 144 8.97e-38 126
Msa1299490 AT1G64230.5 34.574 188 76 2 5 150 2 184 2.25e-36 124
Msa1299490 AT1G64230.4 34.574 188 76 2 5 150 2 184 2.25e-36 124
Msa1299490 AT1G16890.3 38.356 146 81 1 7 143 8 153 1.55e-35 121
Msa1299490 AT3G57870.1 40.000 145 80 2 5 142 6 150 9.72e-35 119
Msa1299490 AT5G59300.1 39.416 137 70 1 25 148 26 162 2.18e-33 115
Msa1299490 AT5G25760.1 39.130 138 80 3 5 138 4 141 5.54e-33 114
Msa1299490 AT5G25760.2 39.130 138 80 3 5 138 4 141 5.54e-33 114
Msa1299490 AT3G46460.1 39.416 137 70 1 25 148 26 162 1.08e-32 114
Msa1299490 AT5G41700.3 44.340 106 59 0 5 110 2 107 1.54e-32 113
Msa1299490 AT3G55380.1 38.686 137 71 1 25 148 27 163 2.44e-32 113
Msa1299490 AT5G53300.3 45.545 101 55 0 5 105 2 102 3.65e-32 110
Msa1299490 AT5G56150.2 43.519 108 61 0 5 112 2 109 1.49e-31 109
Msa1299490 AT3G13550.1 35.766 137 88 0 7 143 39 175 2.14e-31 111
Msa1299490 AT1G36340.2 46.087 115 62 0 31 145 34 148 1.36e-30 108
Msa1299490 AT1G36340.1 46.087 115 62 0 31 145 34 148 1.36e-30 108
Msa1299490 AT5G05080.2 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1299490 AT5G05080.3 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1299490 AT5G05080.1 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1299490 AT5G05080.4 36.029 136 87 0 7 142 13 148 2.69e-30 110
Msa1299490 AT1G16890.1 42.593 108 62 0 36 143 4 111 4.70e-30 105
Msa1299490 AT1G78870.3 40.952 105 62 0 7 111 8 112 6.87e-30 105
Msa1299490 AT1G78870.4 42.593 108 62 0 36 143 4 111 1.11e-29 105
Msa1299490 AT1G78870.1 44.118 102 56 1 36 136 4 105 7.57e-28 100
Msa1299490 AT5G50870.1 35.211 142 83 2 10 142 1 142 2.03e-27 101
Msa1299490 AT3G13550.2 38.393 112 69 0 7 118 39 150 1.36e-26 98.6
Msa1299490 AT2G46030.5 30.872 149 97 3 1 147 1 145 1.72e-26 99.0
Msa1299490 AT2G46030.1 30.872 149 97 3 1 147 1 145 1.72e-26 99.0
Msa1299490 AT3G24515.1 34.641 153 87 2 8 147 10 162 4.11e-26 102
Msa1299490 AT3G46460.3 39.823 113 55 1 49 148 8 120 4.97e-26 95.9
Msa1299490 AT3G46460.2 39.823 113 55 1 49 148 8 120 4.97e-26 95.9
Msa1299490 AT5G50870.2 37.500 128 71 2 10 128 1 128 7.30e-26 97.8
Msa1299490 AT2G46030.3 30.872 149 97 3 1 147 73 217 1.22e-25 98.2
Msa1299490 AT1G63800.1 34.000 150 91 4 1 147 1 145 1.52e-25 96.3
Msa1299490 AT5G41340.1 34.000 150 91 4 1 147 1 145 3.84e-25 95.5
Msa1299490 AT3G55380.2 30.409 171 72 2 25 148 27 197 7.36e-25 95.1
Msa1299490 AT2G46030.4 33.333 120 78 2 30 147 29 148 1.32e-24 94.0
Msa1299490 AT2G46030.2 33.333 120 78 2 30 147 29 148 1.32e-24 94.0
Msa1299490 AT1G63800.2 37.037 108 66 2 42 147 23 130 3.05e-24 92.8
Msa1299490 AT3G24515.3 38.393 112 65 1 40 147 76 187 1.23e-22 92.8
Msa1299490 AT5G41340.2 36.275 102 63 2 48 147 9 110 5.72e-22 86.3
Msa1299490 AT4G36800.2 28.472 144 102 1 8 151 34 176 2.06e-21 85.9
Msa1299490 AT4G36800.1 28.472 144 102 1 8 151 34 176 2.06e-21 85.9
Msa1299490 AT2G18600.1 30.435 138 95 1 8 145 35 171 2.91e-21 85.5
Msa1299490 AT2G18600.2 30.435 138 95 1 8 145 35 171 2.91e-21 85.5
Msa1299490 AT2G32790.1 32.877 146 93 2 1 143 26 169 4.84e-19 79.3
Msa1299490 AT1G45050.1 36.449 107 66 2 1 106 12 117 3.84e-16 71.2
Msa1299490 AT1G75440.1 35.849 106 66 2 1 105 12 116 6.60e-16 70.9
Msa1299490 AT4G36410.1 33.962 106 68 2 1 105 12 116 1.49e-15 69.7
Msa1299490 AT5G50430.1 30.597 134 81 4 7 135 8 134 1.79e-15 71.2
Msa1299490 AT5G50430.2 30.597 134 81 4 7 135 8 134 1.79e-15 71.2
Msa1299490 AT5G42990.1 34.579 107 68 2 1 106 12 117 3.60e-15 68.9
Msa1299490 AT1G17280.9 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.7 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.6 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.2 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.5 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.3 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.1 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.8 31.092 119 73 3 7 120 8 122 4.31e-15 70.1
Msa1299490 AT1G17280.4 27.273 121 81 1 7 120 8 128 2.99e-14 68.2
Msa1299490 AT1G53025.2 31.081 148 81 5 7 133 274 421 6.35e-14 68.2
Msa1299490 AT1G53025.1 31.081 148 81 5 7 133 274 421 6.63e-14 68.2
Msa1299490 AT3G17000.1 28.467 137 85 4 4 135 11 139 1.81e-12 63.5
Msa1299490 AT1G45050.2 35.870 92 57 2 1 91 12 102 6.20e-12 59.3
Msa1299490 AT2G16920.1 30.894 123 73 4 7 118 853 974 9.28e-12 62.4
Msa1299490 AT2G16920.2 30.894 123 73 4 7 118 853 974 9.28e-12 62.4
Msa1299490 AT1G53023.1 27.703 148 86 4 7 133 60 207 1.89e-11 60.8

Find 88 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAATAAAATCTTTGAATTT+TGG 0.108944 8_3:+60760932 Msa1299490:three_prime_UTR
TCAGAGTTTGCAGGAGAATT+TGG 0.151098 8_3:-60760457 None:intergenic
GGCGTGGTTGGTTTCGATTC+TGG 0.209053 8_3:-60756718 None:intergenic
CTGTGGAATGCTGTTATATT+TGG 0.236507 8_3:+60757356 Msa1299490:CDS
CGGCCTGTGAAGGCAGGATT+TGG 0.269187 8_3:+60760588 Msa1299490:three_prime_UTR
CATTCCTTGTTATGAATGTT+AGG 0.279486 8_3:+60760644 Msa1299490:three_prime_UTR
CAGAGTTTGCAGGAGAATTT+GGG 0.319027 8_3:-60760456 None:intergenic
TTCATCTTATCTTTCATTCA+TGG 0.344421 8_3:+60760667 Msa1299490:three_prime_UTR
CTGAATTGGTTATGATCATA+AGG 0.358906 8_3:+60760789 Msa1299490:three_prime_UTR
GACTGACCCGTTAGTGTGTT+TGG 0.359010 8_3:-60756826 None:intergenic
GTTAAAATGAGGAATAAATC+TGG 0.363070 8_3:+60760902 Msa1299490:three_prime_UTR
CGTACAGTGGGTGGCTTGTT+CGG 0.389561 8_3:-60758784 None:intergenic
TAAGTTGTACTTGTCTGAAT+TGG 0.392807 8_3:+60760775 Msa1299490:three_prime_UTR
TACTGTTTGGATGAAACATT+CGG 0.396289 8_3:-60758816 None:intergenic
AGGAATTAGTGGTGCTCCTC+AGG 0.412828 8_3:+60757319 Msa1299490:CDS
TGCAGATGGAAGTATTTGTC+TGG 0.414767 8_3:+60758919 Msa1299490:CDS
CAGTGCAAACAGAATTCAAC+CGG 0.417063 8_3:-60760823 None:intergenic
GGTCATCTTGATATGCTTGT+TGG 0.429544 8_3:+60760565 Msa1299490:three_prime_UTR
ATGGTGAATTTAGAATTTAA+AGG 0.441741 8_3:-60756748 None:intergenic
ACTGACCCGTTAGTGTGTTT+GGG 0.443888 8_3:-60756825 None:intergenic
AAAGCAAGGGCGGGCGGCTA+TGG 0.451384 8_3:-60756767 None:intergenic
ACTGTTTGGATGAAACATTC+GGG 0.455692 8_3:-60758815 None:intergenic
GACCCACCTGCAGGAATTAG+TGG 0.459602 8_3:+60757308 Msa1299490:CDS
TGTGGAATGCTGTTATATTT+GGG 0.459892 8_3:+60757357 Msa1299490:CDS
TTTGCAGGAGAATTTGGGTT+GGG 0.465291 8_3:-60760451 None:intergenic
TCTCTCCCAAACACACTAAC+GGG 0.478747 8_3:+60756820 Msa1299490:five_prime_UTR
TGCGATTCTCACTTCTATCC+AGG 0.480462 8_3:+60758982 Msa1299490:CDS
TGAGTTTGCATGACTCTCAT+AGG 0.483915 8_3:-60760623 None:intergenic
GATAATCCTCGGAAAATTGA+AGG 0.484718 8_3:-60758762 None:intergenic
AGAAATGATATGAGAGTGGA+AGG 0.487174 8_3:+60756681 Msa1299490:five_prime_UTR
AAAGGCAGGCGTGGCGTGGT+TGG 0.487277 8_3:-60756730 None:intergenic
CGAGCCGCTTCAGAGTTTGC+AGG 0.492725 8_3:-60760466 None:intergenic
CTCTGAACAGTTTATGCAGA+TGG 0.494635 8_3:+60758905 Msa1299490:intron
TCGTAGAGTGCGGGAAGTTG+TGG 0.496064 8_3:+60760519 Msa1299490:CDS
ATCGCAGCAACATCATATAT+AGG 0.498248 8_3:-60758965 None:intergenic
AATCCAAATCCTGCCTTCAC+AGG 0.500896 8_3:-60760591 None:intergenic
ATCAATCAAACATTGAATGT+TGG 0.501793 8_3:-60760854 None:intergenic
TGTTGGCGGCCTGTGAAGGC+AGG 0.507951 8_3:+60760582 Msa1299490:three_prime_UTR
TGCTTGTTGGCGGCCTGTGA+AGG 0.512645 8_3:+60760578 Msa1299490:three_prime_UTR
AGAAAGAAATGATATGAGAG+TGG 0.514256 8_3:+60756677 Msa1299490:five_prime_UTR
CCATCCCATGGAGTATCATC+AGG 0.520795 8_3:-60757534 None:intergenic
ATCGAATCGAAAGCAAGGGC+GGG 0.525881 8_3:-60756776 None:intergenic
TGAATTTAGAATTTAAAGGC+AGG 0.525933 8_3:-60756744 None:intergenic
AAATGATATGAGAGTGGAAG+GGG 0.527415 8_3:+60756683 Msa1299490:five_prime_UTR
CTCATCAGTCTCTTCCTAGC+CGG 0.528410 8_3:-60757263 None:intergenic
TATATTACATGAACTGACAT+TGG 0.536734 8_3:-60760705 None:intergenic
AATGGATATTAGTTAAAATG+AGG 0.540225 8_3:+60760891 Msa1299490:three_prime_UTR
CTTCCTAGCCGGAGTCGACA+TGG 0.540330 8_3:-60757252 None:intergenic
CAGAGTTGGACAGCTGACTA+AGG 0.541375 8_3:+60760544 Msa1299490:CDS
CATCTTGATATGCTTGTTGG+CGG 0.544398 8_3:+60760568 Msa1299490:three_prime_UTR
CTCTCTCCCAAACACACTAA+CGG 0.565652 8_3:+60756819 Msa1299490:five_prime_UTR
TGAGTACAATCGTAGAGTGC+GGG 0.565704 8_3:+60760510 Msa1299490:CDS
GCAGTCCTGATGATACTCCA+TGG 0.566145 8_3:+60757529 Msa1299490:intron
TTCTCCTGCAAACTCTGAAG+CGG 0.568993 8_3:+60760462 Msa1299490:CDS
TTGCAACATGACCCACCTGC+AGG 0.570976 8_3:+60757299 Msa1299490:CDS
GGAGCACCACTAATTCCTGC+AGG 0.573494 8_3:-60757314 None:intergenic
GGGAAGTTGTGGAGCAGAGT+TGG 0.573831 8_3:+60760530 Msa1299490:CDS
TTACATGAACTGACATTGGA+AGG 0.574793 8_3:-60760701 None:intergenic
GAGGGATCGAATCGAAAGCA+AGG 0.576553 8_3:-60756781 None:intergenic
GGTGGTATCCATGTCGACTC+CGG 0.579421 8_3:+60757244 Msa1299490:intron
TGGCTTGTTCGGATAATCCT+CGG 0.581252 8_3:-60758773 None:intergenic
GTTTGCAGGAGAATTTGGGT+TGG 0.582593 8_3:-60760452 None:intergenic
GCACCACTAATTCCTGCAGG+TGG 0.583819 8_3:-60757311 None:intergenic
GAACAGTGTTGACAACAATC+CGG 0.587579 8_3:-60760746 None:intergenic
TCATAAGGATTATTTAAACC+CGG 0.588951 8_3:+60760804 Msa1299490:three_prime_UTR
CCTGATGATACTCCATGGGA+TGG 0.589928 8_3:+60757534 Msa1299490:CDS
GATCGAATCGAAAGCAAGGG+CGG 0.598847 8_3:-60756777 None:intergenic
TGGATATATTGCAAAATCAG+TGG 0.599561 8_3:+60758939 Msa1299490:CDS
CGGGAGACAAATCGTACAGT+GGG 0.603878 8_3:-60758796 None:intergenic
ATTTAAAGGCAGGCGTGGCG+TGG 0.605026 8_3:-60756734 None:intergenic
TATCCATGTCGACTCCGGCT+AGG 0.608772 8_3:+60757249 Msa1299490:exon
AAAATTGTATCAATGTGCAC+CGG 0.608962 8_3:+60760727 Msa1299490:three_prime_UTR
ACTGACTCACCTCCATCCCA+TGG 0.613625 8_3:-60757546 None:intergenic
TTAGAATTTAAAGGCAGGCG+TGG 0.614403 8_3:-60756739 None:intergenic
GAAATGATATGAGAGTGGAA+GGG 0.624537 8_3:+60756682 Msa1299490:five_prime_UTR
GAATCGAAAGCAAGGGCGGG+CGG 0.632815 8_3:-60756773 None:intergenic
GAGACAAATCGTACAGTGGG+TGG 0.633963 8_3:-60758793 None:intergenic
CACCACTAATTCCTGCAGGT+GGG 0.640442 8_3:-60757310 None:intergenic
AGGATAACAATATCATGCTG+TGG 0.645864 8_3:+60757339 Msa1299490:CDS
CAGTCCTGATGATACTCCAT+GGG 0.646431 8_3:+60757530 Msa1299490:intron
AGGGATCGAATCGAAAGCAA+GGG 0.652260 8_3:-60756780 None:intergenic
GATATGAGAGTGGAAGGGGA+CGG 0.662403 8_3:+60756687 Msa1299490:five_prime_UTR
GATGATACTCCATGGGATGG+AGG 0.662516 8_3:+60757537 Msa1299490:CDS
GTGAGTACAATCGTAGAGTG+CGG 0.663013 8_3:+60760509 Msa1299490:CDS
TGAACCTAACATTCATAACA+AGG 0.667261 8_3:-60760648 None:intergenic
AGCATGATATTGTTATCCTG+AGG 0.673403 8_3:-60757335 None:intergenic
TCGGGAGACAAATCGTACAG+TGG 0.690605 8_3:-60758797 None:intergenic
AGTGCAAACAGAATTCAACC+GGG 0.747050 8_3:-60760822 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATGAATACAAATAATATA+GGG - chr8_3:60756942-60756961 None:intergenic 15.0%
!! TTGATGAATACAAATAATAT+AGG - chr8_3:60756943-60756962 None:intergenic 15.0%
!!! TGAATATTTTTTTTTTGTCA+AGG + chr8_3:60757862-60757881 Msa1299490:intron 15.0%
!!! AGTTTTTTAAAATCTAAAAC+TGG + chr8_3:60758387-60758406 Msa1299490:intron 15.0%
!! AAATTAATATTGAAAAACAG+AGG - chr8_3:60758454-60758473 None:intergenic 15.0%
!! TTAGATTAATGAAAACTAAA+TGG + chr8_3:60759280-60759299 Msa1299490:intron 15.0%
!! TCCAAATACAATAATATAAT+GGG - chr8_3:60760293-60760312 None:intergenic 15.0%
!! TTCCAAATACAATAATATAA+TGG - chr8_3:60760294-60760313 None:intergenic 15.0%
!!! CTAATAAAATCTTTGAATTT+TGG + chr8_3:60760932-60760951 Msa1299490:three_prime_UTR 15.0%
!!! ATGGTGAATTTAGAATTTAA+AGG - chr8_3:60756751-60756770 None:intergenic 20.0%
!! GCAATAAAATTGATTGAAAA+CGG - chr8_3:60756865-60756884 None:intergenic 20.0%
!!! TCCACTTTTTCTTAATTTTT+GGG + chr8_3:60756980-60756999 Msa1299490:intron 20.0%
!!! ACTTGTCATTAAATTGATTT+TGG + chr8_3:60757073-60757092 Msa1299490:intron 20.0%
!!! ACTTTGTTTAAGTATATGAA+TGG + chr8_3:60757469-60757488 Msa1299490:intron 20.0%
!!! TCTTTGCTCTTAATTTTTTT+AGG + chr8_3:60758235-60758254 Msa1299490:intron 20.0%
!!! GTATATATTTTGAGTTATTC+AGG + chr8_3:60758724-60758743 Msa1299490:intron 20.0%
!! TTGTTCTGACTTATAAATTA+TGG - chr8_3:60759193-60759212 None:intergenic 20.0%
!! AAATAAGTATGCTAAACAAA+GGG - chr8_3:60759573-60759592 None:intergenic 20.0%
!! AAAACTACAAAAAGCAATAT+AGG - chr8_3:60759709-60759728 None:intergenic 20.0%
!!! TTAATTCTCATTTTTCAAAG+AGG - chr8_3:60759940-60759959 None:intergenic 20.0%
!! TATCATTTGATATTTCACAT+AGG - chr8_3:60760047-60760066 None:intergenic 20.0%
!!! ATACAATAATATAATGGGAT+GGG - chr8_3:60760288-60760307 None:intergenic 20.0%
!!! AATACAATAATATAATGGGA+TGG - chr8_3:60760289-60760308 None:intergenic 20.0%
!! TCCCATTATATTATTGTATT+TGG + chr8_3:60760289-60760308 Msa1299490:intron 20.0%
!! AAGTTTGATTCTATTTAGTA+TGG - chr8_3:60760338-60760357 None:intergenic 20.0%
!!! TGATTTTTGTTATGTATGAA+TGG + chr8_3:60760873-60760892 Msa1299490:three_prime_UTR 20.0%
!! AATGGATATTAGTTAAAATG+AGG + chr8_3:60760891-60760910 Msa1299490:three_prime_UTR 20.0%
!! TGAATTTAGAATTTAAAGGC+AGG - chr8_3:60756747-60756766 None:intergenic 25.0%
!!! CTCCACTTTTTCTTAATTTT+TGG + chr8_3:60756979-60756998 Msa1299490:intron 25.0%
!!! CCACTTTTTCTTAATTTTTG+GGG + chr8_3:60756981-60757000 Msa1299490:intron 25.0%
!!! TGTCATTAAATTGATTTTGG+AGG + chr8_3:60757076-60757095 Msa1299490:intron 25.0%
!!! TGGAAAAAATTGATTTTTGC+AGG + chr8_3:60757223-60757242 Msa1299490:intron 25.0%
!!! AAAAAATTGATTTTTGCAGG+TGG + chr8_3:60757226-60757245 Msa1299490:intron 25.0%
!! TTTTTATACTAGTGTCAGTT+AGG + chr8_3:60757607-60757626 Msa1299490:intron 25.0%
! AATACTTGTAACACACAAAA+TGG - chr8_3:60757641-60757660 None:intergenic 25.0%
! TTGAATAGTAAAGTCCAATT+TGG + chr8_3:60757686-60757705 Msa1299490:intron 25.0%
! AATAAGTTGTTTATCCAAAC+AGG - chr8_3:60757896-60757915 None:intergenic 25.0%
!! AAAATCTTTTATAGGATGGA+AGG - chr8_3:60758328-60758347 None:intergenic 25.0%
!! TTCCATCCTATAAAAGATTT+TGG + chr8_3:60758327-60758346 Msa1299490:intron 25.0%
!! TCCATCCTATAAAAGATTTT+GGG + chr8_3:60758328-60758347 Msa1299490:intron 25.0%
!!! TACAACATGTTTTGATTTGA+TGG - chr8_3:60758593-60758612 None:intergenic 25.0%
! ATCAAAACATGTTGTACATT+TGG + chr8_3:60758597-60758616 Msa1299490:intron 25.0%
! TCAAAACATGTTGTACATTT+GGG + chr8_3:60758598-60758617 Msa1299490:intron 25.0%
! TATAATCAATGAGATACACA+TGG + chr8_3:60759223-60759242 Msa1299490:intron 25.0%
! GAAATAAGTATGCTAAACAA+AGG - chr8_3:60759574-60759593 None:intergenic 25.0%
! GAAAGAGAAATTTACAAAAC+AGG - chr8_3:60759749-60759768 None:intergenic 25.0%
!!! AATTTTAAGACTGTTTTGGA+GGG + chr8_3:60759880-60759899 Msa1299490:intron 25.0%
! AATTGCAGGTTATTGAAAAA+TGG - chr8_3:60760181-60760200 None:intergenic 25.0%
!! AGATGCAATAATTTTCTTTG+TGG + chr8_3:60760222-60760241 Msa1299490:intron 25.0%
! TTATTGTATTTGGAAGTTTC+CGG + chr8_3:60760299-60760318 Msa1299490:intron 25.0%
! TTCATCTTATCTTTCATTCA+TGG + chr8_3:60760667-60760686 Msa1299490:three_prime_UTR 25.0%
! TATATTACATGAACTGACAT+TGG - chr8_3:60760708-60760727 None:intergenic 25.0%
! TCATAAGGATTATTTAAACC+CGG + chr8_3:60760804-60760823 Msa1299490:three_prime_UTR 25.0%
! ATCAATCAAACATTGAATGT+TGG - chr8_3:60760857-60760876 None:intergenic 25.0%
! GTTAAAATGAGGAATAAATC+TGG + chr8_3:60760902-60760921 Msa1299490:three_prime_UTR 25.0%
AGAAAGAAATGATATGAGAG+TGG + chr8_3:60756677-60756696 Msa1299490:five_prime_UTR 30.0%
AAATTGATTGAAAACGGAGA+GGG - chr8_3:60756859-60756878 None:intergenic 30.0%
AAAATTGATTGAAAACGGAG+AGG - chr8_3:60756860-60756879 None:intergenic 30.0%
CCCCAAAAATTAAGAAAAAG+TGG - chr8_3:60756984-60757003 None:intergenic 30.0%
AATCTCACAGTGAATTATGT+CGG - chr8_3:60757022-60757041 None:intergenic 30.0%
! TGTCTTGTGGAACTTTTAAT+TGG + chr8_3:60757203-60757222 Msa1299490:intron 30.0%
TGTGGAATGCTGTTATATTT+GGG + chr8_3:60757357-60757376 Msa1299490:CDS 30.0%
! TAGTTTGCAAATAAGAGTTG+TGG - chr8_3:60757581-60757600 None:intergenic 30.0%
!! ATTTTGTCTGAGACTGAATT+GGG - chr8_3:60757733-60757752 None:intergenic 30.0%
!! TATTTTGTCTGAGACTGAAT+TGG - chr8_3:60757734-60757753 None:intergenic 30.0%
! AGTTCCTCGCATTTTATATA+GGG + chr8_3:60757767-60757786 Msa1299490:intron 30.0%
! TAACCCTCAGATTTTCATAT+GGG - chr8_3:60757839-60757858 None:intergenic 30.0%
! TTAACCCTCAGATTTTCATA+TGG - chr8_3:60757840-60757859 None:intergenic 30.0%
!!! TTTTTTTTGTCAAGGACTGA+AGG + chr8_3:60757870-60757889 Msa1299490:intron 30.0%
TATAGCACAAGCAATTATCA+TGG + chr8_3:60757923-60757942 Msa1299490:intron 30.0%
!! TAAGTTTTGTGAGACTGTTT+GGG - chr8_3:60758147-60758166 None:intergenic 30.0%
!! ATAAGTTTTGTGAGACTGTT+TGG - chr8_3:60758148-60758167 None:intergenic 30.0%
TTAAGAGCAAAGACTCAATT+TGG - chr8_3:60758228-60758247 None:intergenic 30.0%
ATGTTAATATTAGCCGAACT+TGG + chr8_3:60758260-60758279 Msa1299490:intron 30.0%
TATGTTGCAAAGAACATCAT+AGG + chr8_3:60758302-60758321 Msa1299490:intron 30.0%
! ACCCAAAATCTTTTATAGGA+TGG - chr8_3:60758332-60758351 None:intergenic 30.0%
! AGCAACCCAAAATCTTTTAT+AGG - chr8_3:60758336-60758355 None:intergenic 30.0%
CAAAACATGTTGTACATTTG+GGG + chr8_3:60758599-60758618 Msa1299490:intron 30.0%
AAAACATGTTGTACATTTGG+GGG + chr8_3:60758600-60758619 Msa1299490:intron 30.0%
TACTGTTTGGATGAAACATT+CGG - chr8_3:60758819-60758838 None:intergenic 30.0%
TCATCCAAACAGTAAGTATT+AGG + chr8_3:60758825-60758844 Msa1299490:intron 30.0%
TGGATATATTGCAAAATCAG+TGG + chr8_3:60758939-60758958 Msa1299490:CDS 30.0%
ACACTATACTAAATGATACC+TGG - chr8_3:60759003-60759022 None:intergenic 30.0%
! ATCTCATTGATTATATGCTC+TGG - chr8_3:60759218-60759237 None:intergenic 30.0%
ACTAAATGGAAATGCCAAAT+AGG + chr8_3:60759294-60759313 Msa1299490:intron 30.0%
CTAAATGGAAATGCCAAATA+GGG + chr8_3:60759295-60759314 Msa1299490:intron 30.0%
AAGGGAATCATTTCAAAACA+GGG - chr8_3:60759356-60759375 None:intergenic 30.0%
AAAGGGAATCATTTCAAAAC+AGG - chr8_3:60759357-60759376 None:intergenic 30.0%
TTCTCATCAAAGAAATTGGA+AGG - chr8_3:60759424-60759443 None:intergenic 30.0%
CCAATTCTCATCAAAGAAAT+TGG - chr8_3:60759428-60759447 None:intergenic 30.0%
CCAATTTCTTTGATGAGAAT+TGG + chr8_3:60759425-60759444 Msa1299490:intron 30.0%
TTGAACTGTGCAATTCATAT+TGG + chr8_3:60759481-60759500 Msa1299490:intron 30.0%
TCTTAAGTCTGATATGCAAA+TGG + chr8_3:60759637-60759656 Msa1299490:intron 30.0%
AAGCAATATAGGAGAAAGAA+GGG - chr8_3:60759698-60759717 None:intergenic 30.0%
AAAGCAATATAGGAGAAAGA+AGG - chr8_3:60759699-60759718 None:intergenic 30.0%
AGAGAAATTTACAAAACAGG+AGG - chr8_3:60759746-60759765 None:intergenic 30.0%
TGTGGAAAACAAACATAACA+AGG - chr8_3:60759815-60759834 None:intergenic 30.0%
GAAAAAGACAGTAAACTTTG+TGG - chr8_3:60759833-60759852 None:intergenic 30.0%
! CAAAGTTTACTGTCTTTTTC+TGG + chr8_3:60759833-60759852 Msa1299490:intron 30.0%
!!! AGGGAATTTTAAGACTGTTT+TGG + chr8_3:60759876-60759895 Msa1299490:intron 30.0%
!!! GAATTTTAAGACTGTTTTGG+AGG + chr8_3:60759879-60759898 Msa1299490:intron 30.0%
!!! ATTTTAAGACTGTTTTGGAG+GGG + chr8_3:60759881-60759900 Msa1299490:intron 30.0%
TGAGAATTAAACCTGTTATG+AGG + chr8_3:60759950-60759969 Msa1299490:intron 30.0%
TGATATGCAAGTATTCAATG+TGG + chr8_3:60760127-60760146 Msa1299490:intron 30.0%
TATTGTATTTGGAAGTTTCC+GGG + chr8_3:60760300-60760319 Msa1299490:intron 30.0%
AGAAAATAATCACACAATGC+TGG - chr8_3:60760390-60760409 None:intergenic 30.0%
! CATTCCTTGTTATGAATGTT+AGG + chr8_3:60760644-60760663 Msa1299490:three_prime_UTR 30.0%
TGAACCTAACATTCATAACA+AGG - chr8_3:60760651-60760670 None:intergenic 30.0%
AAAATTGTATCAATGTGCAC+CGG + chr8_3:60760727-60760746 Msa1299490:three_prime_UTR 30.0%
TAAGTTGTACTTGTCTGAAT+TGG + chr8_3:60760775-60760794 Msa1299490:three_prime_UTR 30.0%
CTGAATTGGTTATGATCATA+AGG + chr8_3:60760789-60760808 Msa1299490:three_prime_UTR 30.0%
AGAAATGATATGAGAGTGGA+AGG + chr8_3:60756681-60756700 Msa1299490:five_prime_UTR 35.0%
GAAATGATATGAGAGTGGAA+GGG + chr8_3:60756682-60756701 Msa1299490:five_prime_UTR 35.0%
AAATGATATGAGAGTGGAAG+GGG + chr8_3:60756683-60756702 Msa1299490:five_prime_UTR 35.0%
TCCAGAGTATAAATCGATCA+CGG - chr8_3:60756907-60756926 None:intergenic 35.0%
TTCAACGTAATTACATGTGC+CGG - chr8_3:60757117-60757136 None:intergenic 35.0%
AGCATGATATTGTTATCCTG+AGG - chr8_3:60757338-60757357 None:intergenic 35.0%
AGGATAACAATATCATGCTG+TGG + chr8_3:60757339-60757358 Msa1299490:CDS 35.0%
CTGTGGAATGCTGTTATATT+TGG + chr8_3:60757356-60757375 Msa1299490:CDS 35.0%
TTTGCAAATAAGAGTTGTGG+TGG - chr8_3:60757578-60757597 None:intergenic 35.0%
! CAGTTCCTCGCATTTTATAT+AGG + chr8_3:60757766-60757785 Msa1299490:intron 35.0%
TGTACCCTATATAAAATGCG+AGG - chr8_3:60757774-60757793 None:intergenic 35.0%
TCTCCCATATGAAAATCTGA+GGG + chr8_3:60757833-60757852 Msa1299490:intron 35.0%
TTAAATCAGCCAATCCAAAC+AGG + chr8_3:60758182-60758201 Msa1299490:intron 35.0%
! ATGCCATCTAAAACCAAGTT+CGG - chr8_3:60758276-60758295 None:intergenic 35.0%
GATAATCCTCGGAAAATTGA+AGG - chr8_3:60758765-60758784 None:intergenic 35.0%
ACTGTTTGGATGAAACATTC+GGG - chr8_3:60758818-60758837 None:intergenic 35.0%
TCCAAACAGTAAGTATTAGG+TGG + chr8_3:60758828-60758847 Msa1299490:intron 35.0%
TCCACCTAATACTTACTGTT+TGG - chr8_3:60758832-60758851 None:intergenic 35.0%
GACACAACTAACAGATAAGA+AGG - chr8_3:60758864-60758883 None:intergenic 35.0%
AGAGCAGCAATTACATTAGT+TGG - chr8_3:60758889-60758908 None:intergenic 35.0%
ATCGCAGCAACATCATATAT+AGG - chr8_3:60758968-60758987 None:intergenic 35.0%
TATGAATGACTCTGTGATGA+AGG + chr8_3:60759250-60759269 Msa1299490:intron 35.0%
AAATGCCAAATAGGGTGAAT+GGG + chr8_3:60759303-60759322 Msa1299490:intron 35.0%
AGGGAATCATTTCAAAACAG+GGG - chr8_3:60759355-60759374 None:intergenic 35.0%
TTAAACTGTTCAGTGCAAAG+GGG - chr8_3:60759391-60759410 None:intergenic 35.0%
CTTAAACTGTTCAGTGCAAA+GGG - chr8_3:60759392-60759411 None:intergenic 35.0%
ACTTAAACTGTTCAGTGCAA+AGG - chr8_3:60759393-60759412 None:intergenic 35.0%
TTCTGTAGTTTCTTTGTTCG+TGG + chr8_3:60759591-60759610 Msa1299490:intron 35.0%
AGCAATATAGGAGAAAGAAG+GGG - chr8_3:60759697-60759716 None:intergenic 35.0%
GCTTGCAACCTAAAAATCTA+AGG + chr8_3:60759784-60759803 Msa1299490:intron 35.0%
! AGGCTCTTCCTTAGATTTTT+AGG - chr8_3:60759795-60759814 None:intergenic 35.0%
ATTCCCTAGTAAACAGAAGT+GGG - chr8_3:60759863-60759882 None:intergenic 35.0%
AATTCCCTAGTAAACAGAAG+TGG - chr8_3:60759864-60759883 None:intergenic 35.0%
TGAAAGGTCAATTAGCTGAA+AGG + chr8_3:60759907-60759926 Msa1299490:intron 35.0%
ACTGTAATATCTGCCAATGA+TGG - chr8_3:60760013-60760032 None:intergenic 35.0%
! GCTTTAATGTTTTCCTGTGT+GGG + chr8_3:60760070-60760089 Msa1299490:intron 35.0%
ATATCACACTCTTGCATCAA+GGG - chr8_3:60760113-60760132 None:intergenic 35.0%
ATTGTATTTGGAAGTTTCCG+GGG + chr8_3:60760301-60760320 Msa1299490:intron 35.0%
AATGCTGGTCAATACATAAC+AGG - chr8_3:60760375-60760394 None:intergenic 35.0%
CAAAGCAATGACTGTCAAAA+AGG - chr8_3:60760427-60760446 None:intergenic 35.0%
TTACATGAACTGACATTGGA+AGG - chr8_3:60760704-60760723 None:intergenic 35.0%
TTAGAATTTAAAGGCAGGCG+TGG - chr8_3:60756742-60756761 None:intergenic 40.0%
AATCGATCACGGAAACAACA+AGG - chr8_3:60756896-60756915 None:intergenic 40.0%
! TCCGTGATCGATTTATACTC+TGG + chr8_3:60756903-60756922 Msa1299490:intron 40.0%
TCTGTGCTTCATAGCATACT+AGG + chr8_3:60757177-60757196 Msa1299490:intron 40.0%
!! GCATACTAGGTTTTGTCTTG+TGG + chr8_3:60757190-60757209 Msa1299490:intron 40.0%
GCTGTTATATTTGGGTGAGT+TGG + chr8_3:60757365-60757384 Msa1299490:intron 40.0%
ATTGGGCCGTAAGAAAAATG+CGG - chr8_3:60757716-60757735 None:intergenic 40.0%
TCCCTCTATCATTATAGCAG+AGG + chr8_3:60757800-60757819 Msa1299490:intron 40.0%
CCCTCTGCTATAATGATAGA+GGG - chr8_3:60757804-60757823 None:intergenic 40.0%
CCCTCTATCATTATAGCAGA+GGG + chr8_3:60757801-60757820 Msa1299490:intron 40.0%
TCCCTCTGCTATAATGATAG+AGG - chr8_3:60757805-60757824 None:intergenic 40.0%
GTCTCCCATATGAAAATCTG+AGG + chr8_3:60757832-60757851 Msa1299490:intron 40.0%
! TTGGTCCCTGACAATTTTCT+CGG - chr8_3:60758209-60758228 None:intergenic 40.0%
!!! TAGCCGAACTTGGTTTTAGA+TGG + chr8_3:60758270-60758289 Msa1299490:intron 40.0%
!!! GATTTTGGGTTGCTCTAGAT+AGG + chr8_3:60758342-60758361 Msa1299490:intron 40.0%
! TAAAACTGGCTTCTGCTTTG+TGG + chr8_3:60758401-60758420 Msa1299490:intron 40.0%
CTCTGAACAGTTTATGCAGA+TGG + chr8_3:60758905-60758924 Msa1299490:intron 40.0%
!! TGCAGATGGAAGTATTTGTC+TGG + chr8_3:60758919-60758938 Msa1299490:CDS 40.0%
GAAATGCCAAATAGGGTGAA+TGG + chr8_3:60759302-60759321 Msa1299490:intron 40.0%
AATGCCAAATAGGGTGAATG+GGG + chr8_3:60759304-60759323 Msa1299490:intron 40.0%
AAGGGGGGTTGAAGAAAAAA+GGG - chr8_3:60759374-60759393 None:intergenic 40.0%
AAAGGGGGGTTGAAGAAAAA+AGG - chr8_3:60759375-60759394 None:intergenic 40.0%
TAAACTGTTCAGTGCAAAGG+GGG - chr8_3:60759390-60759409 None:intergenic 40.0%
GCAATATAGGAGAAAGAAGG+GGG - chr8_3:60759696-60759715 None:intergenic 40.0%
TTCTCCCACTTCTGTTTACT+AGG + chr8_3:60759856-60759875 Msa1299490:intron 40.0%
TCTCCCACTTCTGTTTACTA+GGG + chr8_3:60759857-60759876 Msa1299490:intron 40.0%
! CGCTTTAATGTTTTCCTGTG+TGG + chr8_3:60760069-60760088 Msa1299490:intron 40.0%
CATATCACACTCTTGCATCA+AGG - chr8_3:60760114-60760133 None:intergenic 40.0%
TGTAGAAGCTGAGTAATTGC+AGG - chr8_3:60760195-60760214 None:intergenic 40.0%
CAATTACTCAGCTTCTACAC+TGG + chr8_3:60760196-60760215 Msa1299490:intron 40.0%
GTATGGAGATAAAAGTTCCC+CGG - chr8_3:60760321-60760340 None:intergenic 40.0%
! TTTGCAGGAGAATTTGGGTT+GGG - chr8_3:60760454-60760473 None:intergenic 40.0%
CAGAGTTTGCAGGAGAATTT+GGG - chr8_3:60760459-60760478 None:intergenic 40.0%
TCAGAGTTTGCAGGAGAATT+TGG - chr8_3:60760460-60760479 None:intergenic 40.0%
GGTCATCTTGATATGCTTGT+TGG + chr8_3:60760565-60760584 Msa1299490:three_prime_UTR 40.0%
! CATCTTGATATGCTTGTTGG+CGG + chr8_3:60760568-60760587 Msa1299490:three_prime_UTR 40.0%
TGAGTTTGCATGACTCTCAT+AGG - chr8_3:60760626-60760645 None:intergenic 40.0%
!! GAACAGTGTTGACAACAATC+CGG - chr8_3:60760749-60760768 None:intergenic 40.0%
AGTGCAAACAGAATTCAACC+GGG - chr8_3:60760825-60760844 None:intergenic 40.0%
CAGTGCAAACAGAATTCAAC+CGG - chr8_3:60760826-60760845 None:intergenic 40.0%
AGGGATCGAATCGAAAGCAA+GGG - chr8_3:60756783-60756802 None:intergenic 45.0%
CTCTCTCCCAAACACACTAA+CGG + chr8_3:60756819-60756838 Msa1299490:five_prime_UTR 45.0%
TCTCTCCCAAACACACTAAC+GGG + chr8_3:60756820-60756839 Msa1299490:five_prime_UTR 45.0%
ACTGACCCGTTAGTGTGTTT+GGG - chr8_3:60756828-60756847 None:intergenic 45.0%
! GAGGTTTTGCCTTTTGTTGC+CGG + chr8_3:60757095-60757114 Msa1299490:intron 45.0%
! TTACATGTGCCGGCAACAAA+AGG - chr8_3:60757107-60757126 None:intergenic 45.0%
CTCTGCTTTCTTGTCTGCTT+TGG + chr8_3:60757503-60757522 Msa1299490:intron 45.0%
CAGTCCTGATGATACTCCAT+GGG + chr8_3:60757530-60757549 Msa1299490:intron 45.0%
AAAAATGCGGGGGACCAAAT+TGG - chr8_3:60757703-60757722 None:intergenic 45.0%
TTGGGCCGTAAGAAAAATGC+GGG - chr8_3:60757715-60757734 None:intergenic 45.0%
! TTTGGGATGAAAACAGCGCA+TGG - chr8_3:60758130-60758149 None:intergenic 45.0%
! ACAATTTTCTCGGGCCTGTT+TGG - chr8_3:60758199-60758218 None:intergenic 45.0%
! TGGTCCCTGACAATTTTCTC+GGG - chr8_3:60758208-60758227 None:intergenic 45.0%
GTGCAAATGTAGCCTGTAAC+AGG - chr8_3:60758519-60758538 None:intergenic 45.0%
! GTTGAGCCTTCAATTTTCCG+AGG + chr8_3:60758756-60758775 Msa1299490:CDS 45.0%
!! TGGCTTGTTCGGATAATCCT+CGG - chr8_3:60758776-60758795 None:intergenic 45.0%
TGCGATTCTCACTTCTATCC+AGG + chr8_3:60758982-60759001 Msa1299490:CDS 45.0%
AAACTGTTCAGTGCAAAGGG+GGG - chr8_3:60759389-60759408 None:intergenic 45.0%
TTCTTTGTTCGTGGCTGCTA+AGG + chr8_3:60759600-60759619 Msa1299490:intron 45.0%
!!! TGTTTTGGAGGGGTTGTGAA+AGG + chr8_3:60759891-60759910 Msa1299490:intron 45.0%
!! TTTGGGAAGCACCTCATAAC+AGG - chr8_3:60759964-60759983 None:intergenic 45.0%
! TGTTATGAGGTGCTTCCCAA+AGG + chr8_3:60759963-60759982 Msa1299490:intron 45.0%
CTATCAGCACACAGTCCTTT+GGG - chr8_3:60759981-60760000 None:intergenic 45.0%
ACTATCAGCACACAGTCCTT+TGG - chr8_3:60759982-60760001 None:intergenic 45.0%
ATAGTCTGCAAGGCCATCAT+TGG + chr8_3:60759997-60760016 Msa1299490:intron 45.0%
! TGTTTTCCTGTGTGGGATCA+CGG + chr8_3:60760077-60760096 Msa1299490:intron 45.0%
GAGTCAGGAAGACGAAGATT+AGG - chr8_3:60760252-60760271 None:intergenic 45.0%
GGATGTTAAAGAGTGGAGTC+AGG - chr8_3:60760267-60760286 None:intergenic 45.0%
TGGGATGGGATGTTAAAGAG+TGG - chr8_3:60760274-60760293 None:intergenic 45.0%
GTTTGCAGGAGAATTTGGGT+TGG - chr8_3:60760455-60760474 None:intergenic 45.0%
! TTCTCCTGCAAACTCTGAAG+CGG + chr8_3:60760462-60760481 Msa1299490:CDS 45.0%
GTGAGTACAATCGTAGAGTG+CGG + chr8_3:60760509-60760528 Msa1299490:CDS 45.0%
TGAGTACAATCGTAGAGTGC+GGG + chr8_3:60760510-60760529 Msa1299490:CDS 45.0%
AATCCAAATCCTGCCTTCAC+AGG - chr8_3:60760594-60760613 None:intergenic 45.0%
GATATGAGAGTGGAAGGGGA+CGG + chr8_3:60756687-60756706 Msa1299490:five_prime_UTR 50.0%
ATCGAATCGAAAGCAAGGGC+GGG - chr8_3:60756779-60756798 None:intergenic 50.0%
GATCGAATCGAAAGCAAGGG+CGG - chr8_3:60756780-60756799 None:intergenic 50.0%
GAGGGATCGAATCGAAAGCA+AGG - chr8_3:60756784-60756803 None:intergenic 50.0%
TTGGGAGAGAGAGAGAGAGA+GGG - chr8_3:60756810-60756829 None:intergenic 50.0%
TTTGGGAGAGAGAGAGAGAG+AGG - chr8_3:60756811-60756830 None:intergenic 50.0%
GACTGACCCGTTAGTGTGTT+TGG - chr8_3:60756829-60756848 None:intergenic 50.0%
CTCATCAGTCTCTTCCTAGC+CGG - chr8_3:60757266-60757285 None:intergenic 50.0%
CACCACTAATTCCTGCAGGT+GGG - chr8_3:60757313-60757332 None:intergenic 50.0%
AGGAATTAGTGGTGCTCCTC+AGG + chr8_3:60757319-60757338 Msa1299490:CDS 50.0%
GCAGTCCTGATGATACTCCA+TGG + chr8_3:60757529-60757548 Msa1299490:intron 50.0%
! CCATCCCATGGAGTATCATC+AGG - chr8_3:60757537-60757556 None:intergenic 50.0%
CCTGATGATACTCCATGGGA+TGG + chr8_3:60757534-60757553 Msa1299490:CDS 50.0%
GATGATACTCCATGGGATGG+AGG + chr8_3:60757537-60757556 Msa1299490:CDS 50.0%
! GGTCCCCCGCATTTTTCTTA+CGG + chr8_3:60757707-60757726 Msa1299490:intron 50.0%
TGGGCCGTAAGAAAAATGCG+GGG - chr8_3:60757714-60757733 None:intergenic 50.0%
TCAAGGACTGAAGGCCTGTT+TGG + chr8_3:60757879-60757898 Msa1299490:intron 50.0%
TTTCTCGGGCCTGTTTGGAT+TGG - chr8_3:60758194-60758213 None:intergenic 50.0%
ACAGGCCCGAGAAAATTGTC+AGG + chr8_3:60758200-60758219 Msa1299490:intron 50.0%
CAGGCCCGAGAAAATTGTCA+GGG + chr8_3:60758201-60758220 Msa1299490:intron 50.0%
GAGACAAATCGTACAGTGGG+TGG - chr8_3:60758796-60758815 None:intergenic 50.0%
CGGGAGACAAATCGTACAGT+GGG - chr8_3:60758799-60758818 None:intergenic 50.0%
TCGGGAGACAAATCGTACAG+TGG - chr8_3:60758800-60758819 None:intergenic 50.0%
CTCACCCCATTCACCCTATT+TGG - chr8_3:60759311-60759330 None:intergenic 50.0%
! CTGTGTGCTGATAGTCTGCA+AGG + chr8_3:60759987-60760006 Msa1299490:intron 50.0%
! CTCTTGCATCAAGGGCACAT+TGG - chr8_3:60760105-60760124 None:intergenic 50.0%
CAGAGTTGGACAGCTGACTA+AGG + chr8_3:60760544-60760563 Msa1299490:CDS 50.0%
!! GGCGTGGTTGGTTTCGATTC+TGG - chr8_3:60756721-60756740 None:intergenic 55.0%
ATTTAAAGGCAGGCGTGGCG+TGG - chr8_3:60756737-60756756 None:intergenic 55.0%
GAGAGAGAGAGAGGGAGAGA+GGG - chr8_3:60756802-60756821 None:intergenic 55.0%
AGAGAGAGAGAGAGGGAGAG+AGG - chr8_3:60756803-60756822 None:intergenic 55.0%
!! GGTGGTATCCATGTCGACTC+CGG + chr8_3:60757244-60757263 Msa1299490:intron 55.0%
!! TATCCATGTCGACTCCGGCT+AGG + chr8_3:60757249-60757268 Msa1299490:exon 55.0%
TTGCAACATGACCCACCTGC+AGG + chr8_3:60757299-60757318 Msa1299490:CDS 55.0%
GACCCACCTGCAGGAATTAG+TGG + chr8_3:60757308-60757327 Msa1299490:CDS 55.0%
GCACCACTAATTCCTGCAGG+TGG - chr8_3:60757314-60757333 None:intergenic 55.0%
! GGAGCACCACTAATTCCTGC+AGG - chr8_3:60757317-60757336 None:intergenic 55.0%
! ACTGACTCACCTCCATCCCA+TGG - chr8_3:60757549-60757568 None:intergenic 55.0%
GGGCCGTAAGAAAAATGCGG+GGG - chr8_3:60757713-60757732 None:intergenic 55.0%
CTACCTGATGCGCCTGTTAC+AGG + chr8_3:60758504-60758523 Msa1299490:intron 55.0%
TAGCCTGTAACAGGCGCATC+AGG - chr8_3:60758510-60758529 None:intergenic 55.0%
!! CGTACAGTGGGTGGCTTGTT+CGG - chr8_3:60758787-60758806 None:intergenic 55.0%
TTGGAACCGTGATCCCACAC+AGG - chr8_3:60760086-60760105 None:intergenic 55.0%
TCGTAGAGTGCGGGAAGTTG+TGG + chr8_3:60760519-60760538 Msa1299490:CDS 55.0%
GGGAAGTTGTGGAGCAGAGT+TGG + chr8_3:60760530-60760549 Msa1299490:CDS 55.0%
GAATCGAAAGCAAGGGCGGG+CGG - chr8_3:60756776-60756795 None:intergenic 60.0%
CTTCCTAGCCGGAGTCGACA+TGG - chr8_3:60757255-60757274 None:intergenic 60.0%
CGAGCCGCTTCAGAGTTTGC+AGG - chr8_3:60760469-60760488 None:intergenic 60.0%
! TGCTTGTTGGCGGCCTGTGA+AGG + chr8_3:60760578-60760597 Msa1299490:three_prime_UTR 60.0%
CGGCCTGTGAAGGCAGGATT+TGG + chr8_3:60760588-60760607 Msa1299490:three_prime_UTR 60.0%
AAAGGCAGGCGTGGCGTGGT+TGG - chr8_3:60756733-60756752 None:intergenic 65.0%
AAAGCAAGGGCGGGCGGCTA+TGG - chr8_3:60756770-60756789 None:intergenic 65.0%
TGTTGGCGGCCTGTGAAGGC+AGG + chr8_3:60760582-60760601 Msa1299490:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 60756660 60760959 60756660 ID=Msa1299490;Name=Msa1299490
chr8_3 mRNA 60756660 60760959 60756660 ID=Msa1299490-mRNA-1;Parent=Msa1299490;Name=Msa1299490-mRNA-1;_AED=0.09;_eAED=0.09;_QI=191|1|1|1|0.8|0.66|6|395|152
chr8_3 exon 60756660 60756841 60756660 ID=Msa1299490-mRNA-1:exon:17760;Parent=Msa1299490-mRNA-1
chr8_3 exon 60757245 60757378 60757245 ID=Msa1299490-mRNA-1:exon:17761;Parent=Msa1299490-mRNA-1
chr8_3 exon 60757533 60757558 60757533 ID=Msa1299490-mRNA-1:exon:17762;Parent=Msa1299490-mRNA-1
chr8_3 exon 60758746 60758835 60758746 ID=Msa1299490-mRNA-1:exon:17763;Parent=Msa1299490-mRNA-1
chr8_3 exon 60758915 60759003 60758915 ID=Msa1299490-mRNA-1:exon:17764;Parent=Msa1299490-mRNA-1
chr8_3 exon 60760436 60760959 60760436 ID=Msa1299490-mRNA-1:exon:17765;Parent=Msa1299490-mRNA-1
chr8_3 five_prime_UTR 60756660 60756841 60756660 ID=Msa1299490-mRNA-1:five_prime_utr;Parent=Msa1299490-mRNA-1
chr8_3 five_prime_UTR 60757245 60757253 60757245 ID=Msa1299490-mRNA-1:five_prime_utr;Parent=Msa1299490-mRNA-1
chr8_3 CDS 60757254 60757378 60757254 ID=Msa1299490-mRNA-1:cds;Parent=Msa1299490-mRNA-1
chr8_3 CDS 60757533 60757558 60757533 ID=Msa1299490-mRNA-1:cds;Parent=Msa1299490-mRNA-1
chr8_3 CDS 60758746 60758835 60758746 ID=Msa1299490-mRNA-1:cds;Parent=Msa1299490-mRNA-1
chr8_3 CDS 60758915 60759003 60758915 ID=Msa1299490-mRNA-1:cds;Parent=Msa1299490-mRNA-1
chr8_3 CDS 60760436 60760564 60760436 ID=Msa1299490-mRNA-1:cds;Parent=Msa1299490-mRNA-1
chr8_3 three_prime_UTR 60760565 60760959 60760565 ID=Msa1299490-mRNA-1:three_prime_utr;Parent=Msa1299490-mRNA-1
Gene Sequence

>Msa1299490

ATGTCGACTCCGGCTAGGAAGAGACTGATGAGAGATTTCAAGAGATTGCAACATGACCCACCTGCAGGAATTAGTGGTGCTCCTCAGGATAACAATATCATGCTGTGGAATGCTGTTATATTTGGTCCTGATGATACTCCATGGGATGGAGGCACGTTTAAGTTGAGCCTTCAATTTTCCGAGGATTATCCGAACAAGCCACCCACTGTACGATTTGTCTCCCGAATGTTTCATCCAAACATTTATGCAGATGGAAGTATTTGTCTGGATATATTGCAAAATCAGTGGAGTCCTATATATGATGTTGCTGCGATTCTCACTTCTATCCAGTCATTGCTTTGTGATCCCAACCCAAATTCTCCTGCAAACTCTGAAGCGGCTCGAATGTTCAGTGAGAACAAGCGTGAGTACAATCGTAGAGTGCGGGAAGTTGTGGAGCAGAGTTGGACAGCTGACTAA
Protein sequence

>Msa1299490

MSTPARKRLMRDFKRLQHDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEAARMFSENKREYNRRVREVVEQSWTAD*