AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1264330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1264330 MtrunA17_Chr4g0074021 100.000 159 0 0 1 159 1 159 5.08e-117 327
Msa1264330 MtrunA17_Chr4g0061171 93.711 159 10 0 1 159 1 159 2.71e-109 308
Msa1264330 MtrunA17_Chr3g0091481 92.453 159 12 0 1 159 1 159 1.42e-108 306
Msa1264330 MtrunA17_Chr5g0418211 89.937 159 16 0 1 159 1 159 1.75e-106 300
Msa1264330 MtrunA17_Chr4g0062241 68.627 102 22 1 1 102 1 92 3.17e-45 143
Msa1264330 MtrunA17_Chr4g0074011 47.656 128 19 2 18 145 42 121 1.06e-26 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1264330 AT1G13950.1 91.824 159 12 1 1 159 1 158 4.28e-105 297
Msa1264330 AT1G69410.1 86.792 159 20 1 1 159 1 158 9.26e-102 289
Msa1264330 AT1G26630.1 81.529 157 28 1 1 157 1 156 1.42e-93 268
Msa1264330 AT1G26630.2 84.252 127 19 1 1 127 1 126 6.93e-76 223

Find 65 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATATCCAATAAGTTATTT+TGG 0.141811 8_2:+67843561 Msa1264330:three_prime_UTR
TTCAACCCAAAATAACTTAT+TGG 0.185447 8_2:-67843567 None:intergenic
GATCAATGCGGTTAACTTAT+AGG 0.258167 8_2:+67843348 Msa1264330:three_prime_UTR
ATTATTGAACTCTCTGGTTT+TGG 0.270929 8_2:+67843100 Msa1264330:three_prime_UTR
ATCAATGCGGTTAACTTATA+GGG 0.274474 8_2:+67843349 Msa1264330:three_prime_UTR
CGGTCGATTAGTTGTGTCTA+AGG 0.307333 8_2:+67843456 Msa1264330:three_prime_UTR
AAAGAGCACAGAGGAGAGAT+AGG 0.326363 8_2:-67843184 None:intergenic
AGCCTGTTGAGGGTAGGTTT+TGG 0.330390 8_2:-67840783 None:intergenic
AGGAAGCTTCAGATCATCTT+TGG 0.330486 8_2:-67842758 None:intergenic
ACTTAGGACCAATGTCCTTC+AGG 0.343359 8_2:-67842967 None:intergenic
TTGATTGATATTTCTGAAGA+TGG 0.371340 8_2:+67841886 Msa1264330:CDS
CTTATAGGGGATGCGGATCT+AGG 0.397089 8_2:+67843363 Msa1264330:three_prime_UTR
TCTTTAATTATTGAACTCTC+TGG 0.403510 8_2:+67843094 Msa1264330:three_prime_UTR
CTGAGTAGTTGTGTATATAT+TGG 0.412034 8_2:+67843432 Msa1264330:three_prime_UTR
ATCCATTCATAGATTAAAGA+TGG 0.412301 8_2:+67842878 Msa1264330:intron
TGAGGATCGGATGAATGATT+AGG 0.418550 8_2:+67840595 None:intergenic
GCAGAAAACCTTGCAAGGCC+TGG 0.423629 8_2:-67840843 None:intergenic
TTAAAGAGACAAGATAAATA+TGG 0.437632 8_2:-67843078 None:intergenic
ATCCAAAACCTACCCTCAAC+AGG 0.440341 8_2:+67840781 Msa1264330:CDS
TAAAACTTGGTGTGGAGAAA+AGG 0.444362 8_2:+67843054 Msa1264330:three_prime_UTR
GGATGCTCCGGCATCGGCAG+CGG 0.446886 8_2:-67840762 None:intergenic
GATGCTCCGGCATCGGCAGC+GGG 0.447940 8_2:-67840761 None:intergenic
TTTGAACCCGCTGCCGATGC+CGG 0.456716 8_2:+67840755 Msa1264330:CDS
TTAGTTGTGTCTAAGGGTTA+AGG 0.475769 8_2:+67843463 Msa1264330:three_prime_UTR
CTTAGGACCAATGTCCTTCA+GGG 0.484561 8_2:-67842966 None:intergenic
ATTAAAGATGGATTTGCTGA+TGG 0.488295 8_2:+67842890 Msa1264330:CDS
GGACACTTAAAGAATTCATC+TGG 0.489024 8_2:-67843123 None:intergenic
GAATAGTACCAGCCTGTTGA+GGG 0.502881 8_2:-67840793 None:intergenic
GGTTTCTGTTATGTCTGCGA+TGG 0.503255 8_2:+67842925 Msa1264330:CDS
TATATCTTGGTAGGTTGTTG+AGG 0.505703 8_2:+67841641 Msa1264330:intron
ACATCACAATTGTGGGATGA+AGG 0.511953 8_2:-67841756 None:intergenic
CTAACTTACATCACAATTGT+GGG 0.513530 8_2:-67841763 None:intergenic
TCTAACTTACATCACAATTG+TGG 0.519354 8_2:-67841764 None:intergenic
CATCAAGTCCAGGCCTTGCA+AGG 0.525229 8_2:+67840835 Msa1264330:CDS
GCTGGTACTATTCGCAAGAA+TGG 0.527051 8_2:+67840803 Msa1264330:CDS
CGAATAGTACCAGCCTGTTG+AGG 0.540601 8_2:-67840794 None:intergenic
AGCTTGAAACTCATAAAACT+TGG 0.543878 8_2:+67843041 Msa1264330:three_prime_UTR
TTGATCGCAAGTTATAAAGC+TGG 0.552793 8_2:-67843304 None:intergenic
CGGTTAACTTATAGGGGATG+CGG 0.553112 8_2:+67843356 Msa1264330:three_prime_UTR
GTTTCTGTTATGTCTGCGAT+GGG 0.555762 8_2:+67842926 Msa1264330:CDS
GTGTGTGATGTAGTGTGCAC+TGG 0.568452 8_2:+67843244 Msa1264330:three_prime_UTR
AAGAGCACAGAGGAGAGATA+GGG 0.569296 8_2:-67843183 None:intergenic
GGTCGATTAGTTGTGTCTAA+GGG 0.576561 8_2:+67843457 Msa1264330:three_prime_UTR
AAAACCTACCCTCAACAGGC+TGG 0.584266 8_2:+67840785 Msa1264330:CDS
GATGCTTTCTCTGCGCGCGT+GGG 0.584738 8_2:-67840703 None:intergenic
AGAGCAGATATGTGCCCTGA+AGG 0.589389 8_2:+67842952 Msa1264330:CDS
TGCTGATGGAAAAGATCTCG+TGG 0.593109 8_2:+67842904 Msa1264330:CDS
GAAAAGCAGAAAACCTTGCA+AGG 0.593565 8_2:-67840848 None:intergenic
ATATGTGCCCTGAAGGACAT+TGG 0.593679 8_2:+67842959 Msa1264330:CDS
GTTACATAGTCATCAAGTCC+AGG 0.598114 8_2:+67840825 Msa1264330:CDS
TCGCAAGTTATAAAGCTGGT+TGG 0.598224 8_2:-67843300 None:intergenic
TGATGCTTTCTCTGCGCGCG+TGG 0.598798 8_2:-67840704 None:intergenic
GAAACTCATAAAACTTGGTG+TGG 0.600642 8_2:+67843046 Msa1264330:three_prime_UTR
TGATGATTCACTACTTACTC+AGG 0.602143 8_2:+67842783 Msa1264330:CDS
TCAATGCGGTTAACTTATAG+GGG 0.604359 8_2:+67843350 Msa1264330:three_prime_UTR
GTAAGTAGTGAATCATCAGT+AGG 0.605999 8_2:-67842778 None:intergenic
AGTACCAGCCTGTTGAGGGT+AGG 0.611010 8_2:-67840789 None:intergenic
GTGAGTTTGTTGACTGACAA+TGG 0.614472 8_2:+67842730 Msa1264330:CDS
TATGTGTGAGGAGAAGAAGA+TGG 0.621382 8_2:-67840672 None:intergenic
GTAGTTGTGTATATATTGGA+CGG 0.637381 8_2:+67843436 Msa1264330:three_prime_UTR
TTTATAACTTGCGATCAACA+CGG 0.667104 8_2:+67843309 Msa1264330:three_prime_UTR
GCTCAATAAACTTGTCAGTG+TGG 0.679634 8_2:+67843013 Msa1264330:three_prime_UTR
TGGGTAATGTGTTATGTGTG+AGG 0.693706 8_2:-67840684 None:intergenic
TAATCAGTACGATTCACATG+AGG 0.718954 8_2:-67841859 None:intergenic
ATTAGTTGAAAAGAGCACAG+AGG 0.742860 8_2:-67843193 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATACAAATCTAGAA+GGG - chr8_2:67840880-67840899 None:intergenic 15.0%
!! TAAAAAAATACAAATCTAGA+AGG - chr8_2:67840881-67840900 None:intergenic 15.0%
!! AATAAAAATTGAACATGATT+TGG + chr8_2:67841050-67841069 Msa1264330:intron 15.0%
!!! TTTGATTTGATTGTTTTAAA+CGG + chr8_2:67841082-67841101 Msa1264330:intron 15.0%
!!! TTTGAAACAATTTTTAAAGA+TGG + chr8_2:67841414-67841433 Msa1264330:intron 15.0%
!!! TTTTATAAACTTGATATAAC+TGG + chr8_2:67841476-67841495 Msa1264330:intron 15.0%
!! ATTAAGTTAAAACTTTCAAA+TGG + chr8_2:67841956-67841975 Msa1264330:intron 15.0%
!! ACAAAAATATAAAGTTACAA+GGG - chr8_2:67843147-67843166 None:intergenic 15.0%
!! AACAAAAATATAAAGTTACA+AGG - chr8_2:67843148-67843167 None:intergenic 15.0%
!!! ATATATCCAATAAGTTATTT+TGG + chr8_2:67843561-67843580 Msa1264330:three_prime_UTR 15.0%
!!! TTTAGAATAAAAAGAGAGTT+AGG + chr8_2:67840618-67840637 Msa1264330:five_prime_UTR 20.0%
!! TTAGAATAAAAAGAGAGTTA+GGG + chr8_2:67840619-67840638 Msa1264330:five_prime_UTR 20.0%
!! AAAAAATACAAATCTAGAAG+GGG - chr8_2:67840879-67840898 None:intergenic 20.0%
!! AAAAATTGTTTCAAAACCTA+GGG - chr8_2:67841409-67841428 None:intergenic 20.0%
!! TAAAAATTGTTTCAAAACCT+AGG - chr8_2:67841410-67841429 None:intergenic 20.0%
!! GATATATTGAGAAACAAATA+CGG - chr8_2:67841627-67841646 None:intergenic 20.0%
!!! ATTTGTTTCTCAATATATCT+TGG + chr8_2:67841628-67841647 Msa1264330:intron 20.0%
!!! TTGCTATTGATATTTTCAAT+GGG + chr8_2:67841712-67841731 Msa1264330:CDS 20.0%
!! GGATATATAAAATGAGAATT+AGG + chr8_2:67841977-67841996 Msa1264330:intron 20.0%
!! TGTTATCTTAATGTCTATTA+AGG + chr8_2:67842027-67842046 Msa1264330:intron 20.0%
!! GTTATCTTAATGTCTATTAA+GGG + chr8_2:67842028-67842047 Msa1264330:intron 20.0%
!!! ATAAAACATACTCAGTTTTT+AGG - chr8_2:67842072-67842091 None:intergenic 20.0%
!! AAATATTATAGTCATTCCTT+TGG - chr8_2:67842179-67842198 None:intergenic 20.0%
!!! TTTACATTATGTTTTATGTC+AGG + chr8_2:67842210-67842229 Msa1264330:intron 20.0%
!! TTTAGTGTAATTATAAGTGT+CGG + chr8_2:67842408-67842427 Msa1264330:intron 20.0%
!! AAGTAAAAATAAGATGACAT+AGG + chr8_2:67842513-67842532 Msa1264330:intron 20.0%
!! AGTAAAAATAAGATGACATA+GGG + chr8_2:67842514-67842533 Msa1264330:intron 20.0%
!! AAAGAGACAAAATATATATG+TGG - chr8_2:67842843-67842862 None:intergenic 20.0%
!!! GTCTCTTTAATTTGATTTAT+TGG + chr8_2:67842855-67842874 Msa1264330:intron 20.0%
!! TTAAAGAGACAAGATAAATA+TGG - chr8_2:67843081-67843100 None:intergenic 20.0%
!! TGATGGAGAAAAATAAATTT+AGG + chr8_2:67843216-67843235 Msa1264330:three_prime_UTR 20.0%
! AATTCCTTATTCGATTAATC+TGG + chr8_2:67840900-67840919 Msa1264330:intron 25.0%
! CAAAATCAACACCATATAAA+CGG - chr8_2:67841000-67841019 None:intergenic 25.0%
! TTTACATCTAGATCTAACAT+TGG + chr8_2:67841127-67841146 Msa1264330:intron 25.0%
!! AAGTTAATCTCTTTTCAACA+TGG - chr8_2:67841186-67841205 None:intergenic 25.0%
!!! AATTGTGCTAACTTGTTTAT+TGG + chr8_2:67841598-67841617 Msa1264330:intron 25.0%
!!! ATTGTGCTAACTTGTTTATT+GGG + chr8_2:67841599-67841618 Msa1264330:intron 25.0%
!!! GTTGCTATTGATATTTTCAA+TGG + chr8_2:67841711-67841730 Msa1264330:CDS 25.0%
!! TTGATTGATATTTCTGAAGA+TGG + chr8_2:67841886-67841905 Msa1264330:CDS 25.0%
! GTTATATAGATGAGTTACAT+GGG + chr8_2:67841999-67842018 Msa1264330:intron 25.0%
! TTATATAGATGAGTTACATG+GGG + chr8_2:67842000-67842019 Msa1264330:intron 25.0%
!! AACTGAGTATGTTTTATGTA+AGG + chr8_2:67842075-67842094 Msa1264330:intron 25.0%
!! TTCACAGTAAATTTTACCAA+AGG + chr8_2:67842160-67842179 Msa1264330:intron 25.0%
! ATCCATTCATAGATTAAAGA+TGG + chr8_2:67842878-67842897 Msa1264330:intron 25.0%
! ATCCATCTTTAATCTATGAA+TGG - chr8_2:67842883-67842902 None:intergenic 25.0%
! CATACGAAAAATTACTACTT+AGG - chr8_2:67842986-67843005 None:intergenic 25.0%
! TCTTTAATTATTGAACTCTC+TGG + chr8_2:67843094-67843113 Msa1264330:three_prime_UTR 25.0%
!!! AGTTAAGAGATTTTTTGGTG+CGG - chr8_2:67840649-67840668 None:intergenic 30.0%
!!! GGATGAGTTAAGAGATTTTT+TGG - chr8_2:67840654-67840673 None:intergenic 30.0%
GATTCCAGATTAATCGAATA+AGG - chr8_2:67840907-67840926 None:intergenic 30.0%
TTAACATGCTTCCGTTTATA+TGG + chr8_2:67840986-67841005 Msa1264330:intron 30.0%
!!! CGTTTATATGGTGTTGATTT+TGG + chr8_2:67840998-67841017 Msa1264330:intron 30.0%
!!! ATTTTGGTTGATTGGTAACT+GGG + chr8_2:67841014-67841033 Msa1264330:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG + chr8_2:67841383-67841402 Msa1264330:intron 30.0%
!!! CATTGGAATTTTAGTTTTGC+TGG + chr8_2:67841542-67841561 Msa1264330:intron 30.0%
GTTTCTCAATATATCTTGGT+AGG + chr8_2:67841632-67841651 Msa1264330:intron 30.0%
ACTTCAAAAACAGGAAAACA+TGG + chr8_2:67841669-67841688 Msa1264330:CDS 30.0%
CTAACTTACATCACAATTGT+GGG - chr8_2:67841766-67841785 None:intergenic 30.0%
TCTAACTTACATCACAATTG+TGG - chr8_2:67841767-67841786 None:intergenic 30.0%
TTACTTGTGACAACTTTAGT+AGG + chr8_2:67841927-67841946 Msa1264330:intron 30.0%
GGTTATATAGATGAGTTACA+TGG + chr8_2:67841998-67842017 Msa1264330:intron 30.0%
TATATAGATGAGTTACATGG+GGG + chr8_2:67842001-67842020 Msa1264330:intron 30.0%
TTAATGTCTATTAAGGGATG+AGG + chr8_2:67842034-67842053 Msa1264330:intron 30.0%
TAATGTCTATTAAGGGATGA+GGG + chr8_2:67842035-67842054 Msa1264330:intron 30.0%
TTCTTCATGTTAGGAAAACA+AGG - chr8_2:67842130-67842149 None:intergenic 30.0%
! TAGTTGACTTTCTTCATGTT+AGG - chr8_2:67842139-67842158 None:intergenic 30.0%
TTCTTCATGTCAGGAAAATA+TGG - chr8_2:67842265-67842284 None:intergenic 30.0%
! TATTTGACTTTCTTCATGTC+AGG - chr8_2:67842274-67842293 None:intergenic 30.0%
CCTAAACAAAACATTCATGT+AGG - chr8_2:67842711-67842730 None:intergenic 30.0%
!!! CCTACATGAATGTTTTGTTT+AGG + chr8_2:67842708-67842727 Msa1264330:intron 30.0%
!! ATTAAAGATGGATTTGCTGA+TGG + chr8_2:67842890-67842909 Msa1264330:CDS 30.0%
AGCTTGAAACTCATAAAACT+TGG + chr8_2:67843041-67843060 Msa1264330:three_prime_UTR 30.0%
!! ATTATTGAACTCTCTGGTTT+TGG + chr8_2:67843100-67843119 Msa1264330:three_prime_UTR 30.0%
! TGCTCTTTTCAACTAATTGA+TGG + chr8_2:67843199-67843218 Msa1264330:three_prime_UTR 30.0%
! TTTATAACTTGCGATCAACA+CGG + chr8_2:67843309-67843328 Msa1264330:three_prime_UTR 30.0%
!!! ACACGGTTAACTTTTTTCAA+AGG + chr8_2:67843326-67843345 Msa1264330:three_prime_UTR 30.0%
!!! CTTTTTTCAAAGGATCAATG+CGG + chr8_2:67843336-67843355 Msa1264330:three_prime_UTR 30.0%
ATCAATGCGGTTAACTTATA+GGG + chr8_2:67843349-67843368 Msa1264330:three_prime_UTR 30.0%
CTGAGTAGTTGTGTATATAT+TGG + chr8_2:67843432-67843451 Msa1264330:three_prime_UTR 30.0%
GTAGTTGTGTATATATTGGA+CGG + chr8_2:67843436-67843455 Msa1264330:three_prime_UTR 30.0%
TACAAATCTAGAAGGGGAAA+GGG - chr8_2:67840873-67840892 None:intergenic 35.0%
ATACAAATCTAGAAGGGGAA+AGG - chr8_2:67840874-67840893 None:intergenic 35.0%
!!! TGGTGTTGATTTTGGTTGAT+TGG + chr8_2:67841006-67841025 Msa1264330:intron 35.0%
!!! GATTTTGGTTGATTGGTAAC+TGG + chr8_2:67841013-67841032 Msa1264330:intron 35.0%
TAAACGGAGACATAGACATA+TGG + chr8_2:67841098-67841117 Msa1264330:intron 35.0%
TATATCTTGGTAGGTTGTTG+AGG + chr8_2:67841641-67841660 Msa1264330:intron 35.0%
GAGGTTTCTACTTCAAAAAC+AGG + chr8_2:67841660-67841679 Msa1264330:CDS 35.0%
TAATCAGTACGATTCACATG+AGG - chr8_2:67841862-67841881 None:intergenic 35.0%
TTGTGACAACTTTAGTAGGT+GGG + chr8_2:67841931-67841950 Msa1264330:intron 35.0%
!!! CATACTCAGTTTTTAGGACA+TGG - chr8_2:67842066-67842085 None:intergenic 35.0%
GAGTTTACAACTATGAAGCA+TGG - chr8_2:67842458-67842477 None:intergenic 35.0%
GCACTTGTTTGTATTCACAT+TGG - chr8_2:67842629-67842648 None:intergenic 35.0%
ACAAGTGCACAACAAGAAAA+AGG + chr8_2:67842641-67842660 Msa1264330:intron 35.0%
CAAGTGCACAACAAGAAAAA+GGG + chr8_2:67842642-67842661 Msa1264330:intron 35.0%
TTATGCTATTGTCATTGCAG+TGG + chr8_2:67842673-67842692 Msa1264330:intron 35.0%
GTAAGTAGTGAATCATCAGT+AGG - chr8_2:67842781-67842800 None:intergenic 35.0%
TGATGATTCACTACTTACTC+AGG + chr8_2:67842783-67842802 Msa1264330:CDS 35.0%
TTCACTACTTACTCAGGTAT+TGG + chr8_2:67842789-67842808 Msa1264330:intron 35.0%
TCACTACTTACTCAGGTATT+GGG + chr8_2:67842790-67842809 Msa1264330:intron 35.0%
! GAAACTCATAAAACTTGGTG+TGG + chr8_2:67843046-67843065 Msa1264330:three_prime_UTR 35.0%
!! TAAAACTTGGTGTGGAGAAA+AGG + chr8_2:67843054-67843073 Msa1264330:three_prime_UTR 35.0%
GGACACTTAAAGAATTCATC+TGG - chr8_2:67843126-67843145 None:intergenic 35.0%
! ATTAGTTGAAAAGAGCACAG+AGG - chr8_2:67843196-67843215 None:intergenic 35.0%
TTGATCGCAAGTTATAAAGC+TGG - chr8_2:67843307-67843326 None:intergenic 35.0%
GATCAATGCGGTTAACTTAT+AGG + chr8_2:67843348-67843367 Msa1264330:three_prime_UTR 35.0%
TCAATGCGGTTAACTTATAG+GGG + chr8_2:67843350-67843369 Msa1264330:three_prime_UTR 35.0%
TTAGTTGTGTCTAAGGGTTA+AGG + chr8_2:67843463-67843482 Msa1264330:three_prime_UTR 35.0%
!!! TAAGAGATTTTTTGGTGCGG+TGG - chr8_2:67840646-67840665 None:intergenic 40.0%
TATGTGTGAGGAGAAGAAGA+TGG - chr8_2:67840675-67840694 None:intergenic 40.0%
TGGGTAATGTGTTATGTGTG+AGG - chr8_2:67840687-67840706 None:intergenic 40.0%
GTTACATAGTCATCAAGTCC+AGG + chr8_2:67840825-67840844 Msa1264330:CDS 40.0%
GAAAAGCAGAAAACCTTGCA+AGG - chr8_2:67840851-67840870 None:intergenic 40.0%
TCCCTATATTCCTTGACAAC+AGG + chr8_2:67841517-67841536 Msa1264330:intron 40.0%
CCCTGTTGTCAAGGAATATA+GGG - chr8_2:67841521-67841540 None:intergenic 40.0%
CCCTATATTCCTTGACAACA+GGG + chr8_2:67841518-67841537 Msa1264330:intron 40.0%
TCCCTGTTGTCAAGGAATAT+AGG - chr8_2:67841522-67841541 None:intergenic 40.0%
ACATCACAATTGTGGGATGA+AGG - chr8_2:67841759-67841778 None:intergenic 40.0%
ATCATCTTTGCTCTCTTTGC+AGG + chr8_2:67841834-67841853 Msa1264330:intron 40.0%
CTTGTGACAACTTTAGTAGG+TGG + chr8_2:67841930-67841949 Msa1264330:intron 40.0%
TGCTGTAAACCTATGAAGCA+CGG + chr8_2:67842329-67842348 Msa1264330:intron 40.0%
!! TATAAGTGTCGGTGTTGTGT+CGG + chr8_2:67842419-67842438 Msa1264330:intron 40.0%
!! GTGAGTTTGTTGACTGACAA+TGG + chr8_2:67842730-67842749 Msa1264330:CDS 40.0%
AGGAAGCTTCAGATCATCTT+TGG - chr8_2:67842761-67842780 None:intergenic 40.0%
GTTTCTGTTATGTCTGCGAT+GGG + chr8_2:67842926-67842945 Msa1264330:CDS 40.0%
GCTCAATAAACTTGTCAGTG+TGG + chr8_2:67843013-67843032 Msa1264330:three_prime_UTR 40.0%
TCGCAAGTTATAAAGCTGGT+TGG - chr8_2:67843303-67843322 None:intergenic 40.0%
! GGTCGATTAGTTGTGTCTAA+GGG + chr8_2:67843457-67843476 Msa1264330:three_prime_UTR 40.0%
ATCCAAAACCTACCCTCAAC+AGG + chr8_2:67840781-67840800 Msa1264330:CDS 45.0%
GAATAGTACCAGCCTGTTGA+GGG - chr8_2:67840796-67840815 None:intergenic 45.0%
GCTGGTACTATTCGCAAGAA+TGG + chr8_2:67840803-67840822 Msa1264330:CDS 45.0%
TTCAAAACCTAGGGCCTGTT+TGG - chr8_2:67841400-67841419 None:intergenic 45.0%
CGACATCGTTAGGATGAGTT+AGG + chr8_2:67841446-67841465 Msa1264330:intron 45.0%
TTCCTTGACAACAGGGACAT+TGG + chr8_2:67841525-67841544 Msa1264330:intron 45.0%
TTCCAATGTCCCTGTTGTCA+AGG - chr8_2:67841530-67841549 None:intergenic 45.0%
TGTCTGTATCCGTGCTTCAT+AGG - chr8_2:67842341-67842360 None:intergenic 45.0%
TGCTGATGGAAAAGATCTCG+TGG + chr8_2:67842904-67842923 Msa1264330:CDS 45.0%
GGTTTCTGTTATGTCTGCGA+TGG + chr8_2:67842925-67842944 Msa1264330:CDS 45.0%
ATATGTGCCCTGAAGGACAT+TGG + chr8_2:67842959-67842978 Msa1264330:CDS 45.0%
CTTAGGACCAATGTCCTTCA+GGG - chr8_2:67842969-67842988 None:intergenic 45.0%
ACTTAGGACCAATGTCCTTC+AGG - chr8_2:67842970-67842989 None:intergenic 45.0%
! AAGAGCACAGAGGAGAGATA+GGG - chr8_2:67843186-67843205 None:intergenic 45.0%
! AAAGAGCACAGAGGAGAGAT+AGG - chr8_2:67843187-67843206 None:intergenic 45.0%
CGGTTAACTTATAGGGGATG+CGG + chr8_2:67843356-67843375 Msa1264330:three_prime_UTR 45.0%
! CGGTCGATTAGTTGTGTCTA+AGG + chr8_2:67843456-67843475 Msa1264330:three_prime_UTR 45.0%
!!! AGGGTAGGTTTTGGATGCTC+CGG - chr8_2:67840777-67840796 None:intergenic 50.0%
! AGCCTGTTGAGGGTAGGTTT+TGG - chr8_2:67840786-67840805 None:intergenic 50.0%
AAAACCTACCCTCAACAGGC+TGG + chr8_2:67840785-67840804 Msa1264330:CDS 50.0%
CGAATAGTACCAGCCTGTTG+AGG - chr8_2:67840797-67840816 None:intergenic 50.0%
AGTCAATCCAAACAGGCCCT+AGG + chr8_2:67841390-67841409 Msa1264330:intron 50.0%
GACACGTAGTCGACATCGTT+AGG + chr8_2:67841436-67841455 Msa1264330:intron 50.0%
TGAAGCACGGATACAGACAC+CGG + chr8_2:67842342-67842361 Msa1264330:intron 50.0%
AGAGCAGATATGTGCCCTGA+AGG + chr8_2:67842952-67842971 Msa1264330:CDS 50.0%
GTGTGTGATGTAGTGTGCAC+TGG + chr8_2:67843244-67843263 Msa1264330:three_prime_UTR 50.0%
CTTATAGGGGATGCGGATCT+AGG + chr8_2:67843363-67843382 Msa1264330:three_prime_UTR 50.0%
!!! GGTTTTGGATGCTCCGGCAT+CGG - chr8_2:67840771-67840790 None:intergenic 55.0%
AGTACCAGCCTGTTGAGGGT+AGG - chr8_2:67840792-67840811 None:intergenic 55.0%
CATCAAGTCCAGGCCTTGCA+AGG + chr8_2:67840835-67840854 Msa1264330:CDS 55.0%
GCAGAAAACCTTGCAAGGCC+TGG - chr8_2:67840846-67840865 None:intergenic 55.0%
ACGGATACAGACACCGGACA+CGG + chr8_2:67842348-67842367 Msa1264330:intron 55.0%
GTTATGCCAGTGTCCGTGTC+CGG - chr8_2:67842364-67842383 None:intergenic 55.0%
GATGCTTTCTCTGCGCGCGT+GGG - chr8_2:67840706-67840725 None:intergenic 60.0%
TGATGCTTTCTCTGCGCGCG+TGG - chr8_2:67840707-67840726 None:intergenic 60.0%
TTTGAACCCGCTGCCGATGC+CGG + chr8_2:67840755-67840774 Msa1264330:CDS 60.0%
CAGACACCGGACACGGACAC+TGG + chr8_2:67842355-67842374 Msa1264330:intron 65.0%
!! GATGCTCCGGCATCGGCAGC+GGG - chr8_2:67840764-67840783 None:intergenic 70.0%
!! GGATGCTCCGGCATCGGCAG+CGG - chr8_2:67840765-67840784 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 67840607 67843584 67840607 ID=Msa1264330;Name=Msa1264330
chr8_2 mRNA 67840607 67843584 67840607 ID=Msa1264330-mRNA-1;Parent=Msa1264330;Name=Msa1264330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=127|1|1|1|1|1|5|593|159
chr8_2 exon 67840607 67840856 67840607 ID=Msa1264330-mRNA-1:exon:22343;Parent=Msa1264330-mRNA-1
chr8_2 exon 67841654 67841776 67841654 ID=Msa1264330-mRNA-1:exon:22344;Parent=Msa1264330-mRNA-1
chr8_2 exon 67841856 67841912 67841856 ID=Msa1264330-mRNA-1:exon:22345;Parent=Msa1264330-mRNA-1
chr8_2 exon 67842730 67842804 67842730 ID=Msa1264330-mRNA-1:exon:22346;Parent=Msa1264330-mRNA-1
chr8_2 exon 67842890 67843584 67842890 ID=Msa1264330-mRNA-1:exon:22347;Parent=Msa1264330-mRNA-1
chr8_2 five_prime_UTR 67840607 67840733 67840607 ID=Msa1264330-mRNA-1:five_prime_utr;Parent=Msa1264330-mRNA-1
chr8_2 CDS 67840734 67840856 67840734 ID=Msa1264330-mRNA-1:cds;Parent=Msa1264330-mRNA-1
chr8_2 CDS 67841654 67841776 67841654 ID=Msa1264330-mRNA-1:cds;Parent=Msa1264330-mRNA-1
chr8_2 CDS 67841856 67841912 67841856 ID=Msa1264330-mRNA-1:cds;Parent=Msa1264330-mRNA-1
chr8_2 CDS 67842730 67842804 67842730 ID=Msa1264330-mRNA-1:cds;Parent=Msa1264330-mRNA-1
chr8_2 CDS 67842890 67842991 67842890 ID=Msa1264330-mRNA-1:cds;Parent=Msa1264330-mRNA-1
chr8_2 three_prime_UTR 67842992 67843584 67842992 ID=Msa1264330-mRNA-1:three_prime_utr;Parent=Msa1264330-mRNA-1
Gene Sequence

>Msa1264330

ATGTCTGACGAAGAACATCACTTTGAACCCGCTGCCGATGCCGGAGCATCCAAAACCTACCCTCAACAGGCTGGTACTATTCGCAAGAATGGTTACATAGTCATCAAGTCCAGGCCTTGCAAGGTTGTTGAGGTTTCTACTTCAAAAACAGGAAAACATGGACATGCAAAGTGTCACTTTGTTGCTATTGATATTTTCAATGGGAAAAAACTTGAAGATATTGTTCCTTCATCCCACAATTGTGATGTTCCTCATGTGAATCGTACTGATTATCAGTTGATTGATATTTCTGAAGATGGATTTGTGAGTTTGTTGACTGACAATGGAAGTACCAAAGATGATCTGAAGCTTCCTACTGATGATTCACTACTTACTCAGATTAAAGATGGATTTGCTGATGGAAAAGATCTCGTGGTTTCTGTTATGTCTGCGATGGGTGAAGAGCAGATATGTGCCCTGAAGGACATTGGTCCTAAGTAG
Protein sequence

>Msa1264330

MSDEEHHFEPAADAGASKTYPQQAGTIRKNGYIVIKSRPCKVVEVSTSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGPK*