AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1227710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227710 MtrunA17_Chr8g0334461 91.696 289 19 2 2 287 1 287 2.70e-175 485
Msa1227710 MtrunA17_Chr8g0340031 60.455 220 84 2 67 286 33 249 7.40e-98 287
Msa1227710 MtrunA17_Chr8g0356021 46.512 215 111 3 73 287 49 259 2.66e-64 202
Msa1227710 MtrunA17_Chr8g0340821 43.519 216 116 4 73 287 51 261 1.09e-61 196
Msa1227710 MtrunA17_Chr8g0340811 43.519 216 116 4 73 287 54 264 2.41e-61 195
Msa1227710 MtrunA17_Chr8g0340021 63.077 130 48 0 157 286 23 152 7.01e-60 187
Msa1227710 MtrunA17_Chr6g0487381 43.578 218 117 4 70 286 39 251 9.70e-58 185
Msa1227710 MtrunA17_Chr8g0334471 98.592 71 1 0 159 229 7 77 2.88e-49 157
Msa1227710 MtrunA17_Chr1g0168451 37.615 218 133 3 70 287 52 266 4.93e-48 160
Msa1227710 MtrunA17_Chr1g0186301 36.364 220 135 5 68 287 48 262 1.22e-42 147
Msa1227710 MtrunA17_Chr7g0265641 38.739 111 66 2 160 269 6 115 7.04e-20 83.6
Msa1227710 MtrunA17_Chr8g0356651 43.182 88 46 2 73 160 52 135 1.83e-18 80.5
Msa1227710 MtrunA17_Chr8g0356661 57.407 54 23 0 231 284 1 54 3.74e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1227710 AT2G38600.2 57.983 238 95 3 51 286 16 250 1.33e-94 280
Msa1227710 AT2G38600.1 57.983 238 95 3 51 286 16 250 1.33e-94 280
Msa1227710 AT4G29270.1 42.202 218 122 3 70 287 43 256 2.36e-62 197
Msa1227710 AT4G29260.1 43.891 221 120 3 66 286 38 254 3.08e-61 195
Msa1227710 AT4G25150.1 43.925 214 116 3 73 286 50 259 1.44e-60 193
Msa1227710 AT5G51260.1 39.506 243 139 4 44 286 22 256 2.17e-59 190
Msa1227710 AT1G04040.1 36.948 249 148 5 46 287 25 271 2.50e-50 167
Msa1227710 AT5G44020.1 37.273 220 134 4 70 287 55 272 5.11e-47 159
Msa1227710 AT5G24780.1 35.648 216 131 7 73 286 59 268 1.36e-39 139
Msa1227710 AT5G24770.1 36.111 216 130 7 73 286 54 263 2.02e-39 139
Msa1227710 AT5G51260.2 36.022 186 111 4 44 229 22 199 1.19e-37 132
Msa1227710 AT5G24780.2 32.298 161 103 5 73 231 59 215 3.21e-24 98.2
Msa1227710 AT5G24770.2 33.333 159 100 5 73 229 54 208 1.53e-23 95.9

Find 86 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTGTCCGTAGTGATTT+TGG 0.138152 8_2:+3072152 None:intergenic
TCATCAAGATGCTTGGATTT+TGG 0.148705 8_2:-3072471 Msa1227710:CDS
AAGAGGAAGGGTATAGAATA+TGG 0.225208 8_2:-3069117 Msa1227710:CDS
AAAGCAGAATATATATAATA+AGG 0.234689 8_2:+3072936 None:intergenic
TTGTTAATTACCTTAAAATA+AGG 0.238981 8_2:+3072100 None:intergenic
AATCTCTTAGCTTTATAATA+AGG 0.250871 8_2:+3072419 None:intergenic
TATTATAAAGCTAAGAGATT+TGG 0.260805 8_2:-3072416 Msa1227710:CDS
GAAGAAGAAGAATCTAATTC+AGG 0.315145 8_2:+3072722 None:intergenic
ATTATAAAGCTAAGAGATTT+GGG 0.319914 8_2:-3072415 Msa1227710:intron
TGATCTCCAAGGGGAGTGTT+TGG 0.332481 8_2:-3069071 Msa1227710:CDS
TTCTTCGTCGTCGGAGATGA+TGG 0.343997 8_2:-3072702 Msa1227710:CDS
AATATATGAACAAATTGATT+AGG 0.351239 8_2:+3072903 None:intergenic
CTTAGTACCACAGGGTTTGC+AGG 0.358088 8_2:+3072246 None:intergenic
AATTTGTTGGCAATGGCATT+AGG 0.359973 8_2:+3072980 None:intergenic
TTCAAAGTGTTCTTAGTAAC+GGG 0.404621 8_2:-3072195 Msa1227710:CDS
TTGAACATCGCCGAATCAAA+TGG 0.405341 8_2:+3072294 None:intergenic
GAAATGCAGTACATAGGATT+GGG 0.410928 8_2:+3069028 None:intergenic
AAGCAGAATATATATAATAA+GGG 0.423271 8_2:+3072937 None:intergenic
GATCTCCAAGGGGAGTGTTT+GGG 0.434280 8_2:-3069070 Msa1227710:CDS
TGCCATTGCCAACAAATTCT+CGG 0.438024 8_2:-3072975 Msa1227710:five_prime_UTR
CTAGAAGAAGATTAGAAGAA+AGG 0.444493 8_2:+3073043 None:intergenic
AAAGATATTGCAAGCAGTAT+TGG 0.455653 8_2:-3072852 Msa1227710:CDS
CAAACAATAATGTGCGTCCT+TGG 0.456134 8_2:-3072628 Msa1227710:CDS
CGGAAGGAGATTGAAGAGGA+AGG 0.460525 8_2:-3069130 Msa1227710:CDS
TATAGAATATGGGGAAATGT+AGG 0.464952 8_2:-3069106 Msa1227710:CDS
AGAGGAAGGGTATAGAATAT+GGG 0.466203 8_2:-3069116 Msa1227710:CDS
TTTCTTACATGGATTGATGA+TGG 0.469031 8_2:+3073079 None:intergenic
ATTTGATTCGGCGATGTTCA+AGG 0.469405 8_2:-3072292 Msa1227710:CDS
AGAAATGCAGTACATAGGAT+TGG 0.475430 8_2:+3069027 None:intergenic
TGAACATCGCCGAATCAAAT+GGG 0.497521 8_2:+3072295 None:intergenic
CTACTACTCATCAAGATGCT+TGG 0.498659 8_2:-3072478 Msa1227710:CDS
CAGATGCGACCCATTTGATT+CGG 0.501157 8_2:-3072304 Msa1227710:CDS
TTAAACAATCTTAGTACCAC+AGG 0.502868 8_2:+3072237 None:intergenic
ATGAGAGGTATGGGCTGAGC+TGG 0.503269 8_2:-3072667 Msa1227710:CDS
CATGTTGAGAATTACATGAT+TGG 0.504365 8_2:-3072578 Msa1227710:CDS
AAATGTGTCCGAGAATTTGT+TGG 0.506283 8_2:+3072967 None:intergenic
ATTCAAAGTGTTCTTAGTAA+CGG 0.509245 8_2:-3072196 Msa1227710:CDS
GAATATATATAATAAGGGAT+TGG 0.510983 8_2:+3072942 None:intergenic
GTTGGTAGCCGATGAATCCT+TGG 0.512582 8_2:+3072124 None:intergenic
ATTCGGCGATGTTCAAGGCA+TGG 0.525698 8_2:-3072287 Msa1227710:CDS
GTTGATGATGATGAGAGGTA+TGG 0.526024 8_2:-3072677 Msa1227710:CDS
CCCCTTGGAGATCAGTCCAC+TGG 0.527498 8_2:+3069080 None:intergenic
TTCAAGGCATGGATTAATCA+AGG 0.531044 8_2:-3072276 Msa1227710:CDS
GTTGAGAATTACATGATTGG+TGG 0.532110 8_2:-3072575 Msa1227710:CDS
TGAGTAGTAGAAGTAGAAAG+AGG 0.532684 8_2:+3072491 None:intergenic
TTGCATAACCAAGGATTCAT+CGG 0.534552 8_2:-3072132 Msa1227710:CDS
TTTAAAACGTTGATAGACAA+AGG 0.537850 8_2:-3072219 Msa1227710:CDS
GGAAGGAGATTGAAGAGGAA+GGG 0.539212 8_2:-3069129 Msa1227710:CDS
ATATTGCAAGCAGTATTGGC+GGG 0.541832 8_2:-3072848 Msa1227710:CDS
TGAAATACAAGTCATCGATT+CGG 0.550970 8_2:-3069150 Msa1227710:CDS
AATCAAGAAATGCAGTACAT+AGG 0.552007 8_2:+3069022 None:intergenic
GATTGCCCAAACACTCCCCT+TGG 0.552792 8_2:+3069065 None:intergenic
GTATGGGCTGAGCTGGAGGT+TGG 0.553413 8_2:-3072660 Msa1227710:CDS
TTTGTTGAGTACTACACAAG+TGG 0.559383 8_2:+3068982 None:intergenic
GTCCGAGAATTTGTTGGCAA+TGG 0.560861 8_2:+3072973 None:intergenic
GATATTGCAAGCAGTATTGG+CGG 0.561358 8_2:-3072849 Msa1227710:CDS
GCTGAGCTGGAGGTTGGCAA+TGG 0.567591 8_2:-3072654 Msa1227710:CDS
TTAGTACCACAGGGTTTGCA+GGG 0.578209 8_2:+3072247 None:intergenic
ACCAGTGGACTGATCTCCAA+GGG 0.586615 8_2:-3069081 Msa1227710:CDS
TTCGTCGTCGGAGATGATGG+TGG 0.596102 8_2:-3072699 Msa1227710:CDS
GGCAGGAGTGTGAAATACAA+AGG 0.596346 8_2:-3069184 Msa1227710:intron
ATTACCTTAAAATAAGGCGT+TGG 0.596763 8_2:+3072106 None:intergenic
GAGGAAGGGTATAGAATATG+GGG 0.605333 8_2:-3069115 Msa1227710:CDS
TTGATGATGATGAGAGGTAT+GGG 0.609061 8_2:-3072676 Msa1227710:CDS
TATTAAGCAATCTAGAGTTG+AGG 0.612268 8_2:-3072805 Msa1227710:CDS
CGAAGTGGGACTGTCTTCCA+AGG 0.617163 8_2:+3072611 None:intergenic
TATTGCAAGCAGTATTGGCG+GGG 0.620877 8_2:-3072847 Msa1227710:CDS
AACACTTGCCAAAATCACTA+CGG 0.621427 8_2:-3072160 Msa1227710:CDS
AGATTAGAAGAAAGGTACTA+CGG 0.624949 8_2:+3073051 None:intergenic
ACGGACAATTTGCATAACCA+AGG 0.630462 8_2:-3072141 Msa1227710:CDS
CATGCTTGTAACAACGAAGT+GGG 0.631275 8_2:+3072597 None:intergenic
ATACAAGTCATCGATTCGGA+AGG 0.633877 8_2:-3069146 Msa1227710:CDS
ACATGCTTGTAACAACGAAG+TGG 0.634605 8_2:+3072596 None:intergenic
AGAGGTATGGGCTGAGCTGG+AGG 0.636934 8_2:-3072664 Msa1227710:CDS
GACCAGTGGACTGATCTCCA+AGG 0.637891 8_2:-3069082 Msa1227710:CDS
AAATGCAGTACATAGGATTG+GGG 0.643105 8_2:+3069029 None:intergenic
GATTCGGAAGGAGATTGAAG+AGG 0.657286 8_2:-3069134 Msa1227710:CDS
ACACAATTTCATCCACGATG+AGG 0.660835 8_2:+3072531 None:intergenic
TAAACAATCTTAGTACCACA+GGG 0.665394 8_2:+3072238 None:intergenic
GGGGAAATGTAGGAGACCAG+TGG 0.670649 8_2:-3069096 Msa1227710:CDS
TGGTGGTTGATGATGATGAG+AGG 0.675061 8_2:-3072682 Msa1227710:CDS
TTAGTAACGGGTAGATACGA+AGG 0.691062 8_2:-3072183 Msa1227710:CDS
GAAATACAAAGGACAAAGTG+CGG 0.704065 8_2:-3069173 Msa1227710:CDS
CCAGTGGACTGATCTCCAAG+GGG 0.746364 8_2:-3069080 Msa1227710:CDS
GCATGACATGAACCTCATCG+TGG 0.754708 8_2:-3072543 Msa1227710:CDS
AATGTGCCCTGCAAACCCTG+TGG 0.782788 8_2:-3072253 Msa1227710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTATGATATGATAT+TGG - chr8_2:3069688-3069707 Msa1227710:intron 10.0%
!!! TTTTTTTATGATATGATATT+GGG - chr8_2:3069689-3069708 Msa1227710:intron 10.0%
!! ATATATATAATTGGAAATTA+AGG + chr8_2:3070542-3070561 None:intergenic 10.0%
!!! TAATTGTTAAAATTGTTTTA+TGG + chr8_2:3071386-3071405 None:intergenic 10.0%
!!! TTAACAATTAATTTTCTTTT+TGG - chr8_2:3071396-3071415 Msa1227710:intron 10.0%
!! TGTATTCAATTAATAAAAAT+TGG - chr8_2:3071793-3071812 Msa1227710:intron 10.0%
!! GTATTCAATTAATAAAAATT+GGG - chr8_2:3071794-3071813 Msa1227710:intron 10.0%
!! AAGCAGAATATATATAATAA+GGG + chr8_2:3069085-3069104 None:intergenic 15.0%
!! AAAGCAGAATATATATAATA+AGG + chr8_2:3069086-3069105 None:intergenic 15.0%
!! AATATATGAACAAATTGATT+AGG + chr8_2:3069119-3069138 None:intergenic 15.0%
!!! TTTTTATTATATGTTAGACT+TGG - chr8_2:3069651-3069670 Msa1227710:intron 15.0%
!!! TTTTATTATATGTTAGACTT+GGG - chr8_2:3069652-3069671 Msa1227710:intron 15.0%
!! TTGTTAATTACCTTAAAATA+AGG + chr8_2:3069922-3069941 None:intergenic 15.0%
!!! TTATTTTTTTTTGTGATGTT+CGG - chr8_2:3070130-3070149 Msa1227710:intron 15.0%
!! TTTATAACAATGATATTTGT+GGG - chr8_2:3070231-3070250 Msa1227710:intron 15.0%
!!! ACTACTACATTTTTTTTTAA+GGG + chr8_2:3070291-3070310 None:intergenic 15.0%
!!! TACTACTACATTTTTTTTTA+AGG + chr8_2:3070292-3070311 None:intergenic 15.0%
!!! TGTTAACTCTAAAATAAAAT+AGG + chr8_2:3070589-3070608 None:intergenic 15.0%
!!! ATATACTCTTTTTGTTTTAT+AGG + chr8_2:3070737-3070756 None:intergenic 15.0%
!!! TGCATTTTCTAATTTTATTA+CGG - chr8_2:3070853-3070872 Msa1227710:intron 15.0%
!! AACAATTTCATAATATCAAA+AGG + chr8_2:3071422-3071441 None:intergenic 15.0%
!!! TAAGCAAAAATTAACTTTTT+AGG - chr8_2:3071547-3071566 Msa1227710:intron 15.0%
!!! TAAGCAAAAATCAATTTTTA+AGG + chr8_2:3071636-3071655 None:intergenic 15.0%
!!! AATTTATTGAAAGTATAAAC+AGG + chr8_2:3071733-3071752 None:intergenic 15.0%
!!! TGTATTCAATTAATTTTTTG+AGG - chr8_2:3071767-3071786 Msa1227710:intron 15.0%
!!! GTATTCAATTAATTTTTTGA+GGG - chr8_2:3071768-3071787 Msa1227710:intron 15.0%
!!! TTTGTTAACCTAATTTTTAT+GGG + chr8_2:3071996-3072015 None:intergenic 15.0%
!! AAATTCGTAATTAACAATAA+AGG - chr8_2:3072088-3072107 Msa1227710:intron 15.0%
!! AATTCGTAATTAACAATAAA+GGG - chr8_2:3072089-3072108 Msa1227710:intron 15.0%
!!! AACTTTTTTTTTCTAACTTA+CGG - chr8_2:3072142-3072161 Msa1227710:CDS 15.0%
!! AGATAAGAAAATATTAGTAT+CGG + chr8_2:3072368-3072387 None:intergenic 15.0%
!!! CTTTTAACCTTAATATTTAT+TGG - chr8_2:3072808-3072827 Msa1227710:CDS 15.0%
!! GAATATATATAATAAGGGAT+TGG + chr8_2:3069080-3069099 None:intergenic 20.0%
!!! AATCTCTTAGCTTTATAATA+AGG + chr8_2:3069603-3069622 None:intergenic 20.0%
!! TATTATAAAGCTAAGAGATT+TGG - chr8_2:3069603-3069622 Msa1227710:intron 20.0%
!! ATTATAAAGCTAAGAGATTT+GGG - chr8_2:3069604-3069623 Msa1227710:intron 20.0%
!! TTTATTATATGTTAGACTTG+GGG - chr8_2:3069653-3069672 Msa1227710:intron 20.0%
!! ACAGAAAATTATTAATGACT+AGG - chr8_2:3070101-3070120 Msa1227710:intron 20.0%
!!! TATTTTTTTTTGTGATGTTC+GGG - chr8_2:3070131-3070150 Msa1227710:intron 20.0%
!! GTTTATAACAATGATATTTG+TGG - chr8_2:3070230-3070249 Msa1227710:intron 20.0%
!! TTATAACAATGATATTTGTG+GGG - chr8_2:3070232-3070251 Msa1227710:intron 20.0%
!! AAAAAAAAATGTAGTAGTAG+TGG - chr8_2:3070293-3070312 Msa1227710:intron 20.0%
!! ACAGATAACTATACTAAAAT+CGG + chr8_2:3070393-3070412 None:intergenic 20.0%
!! CATAGCTTGATATATATAAT+TGG + chr8_2:3070551-3070570 None:intergenic 20.0%
!!! GATTTGGTTAAAATAATACA+AGG + chr8_2:3070694-3070713 None:intergenic 20.0%
!!! AAGAGTATATTTAATTGACT+TGG - chr8_2:3070747-3070766 Msa1227710:intron 20.0%
!!! ATATATCCACATTTTCTATT+GGG - chr8_2:3070938-3070957 Msa1227710:intron 20.0%
!!! TATTGGGATATTTTTTGTAT+TGG - chr8_2:3070954-3070973 Msa1227710:intron 20.0%
!!! AGGTGTTTTATTTTATTTGA+GGG + chr8_2:3071128-3071147 None:intergenic 20.0%
!!! ACTTTTCTTTTGAATTTTAG+AGG + chr8_2:3071148-3071167 None:intergenic 20.0%
!! CAAAACTTACTCATTATATT+TGG + chr8_2:3071495-3071514 None:intergenic 20.0%
!! CATTCATTAAATGATGTATT+TGG - chr8_2:3071572-3071591 Msa1227710:intron 20.0%
!! AATTAATTATGAAGATTGCA+TGG + chr8_2:3071690-3071709 None:intergenic 20.0%
!!! CAGGTTTAATTTTTTTTTGA+AGG + chr8_2:3071714-3071733 None:intergenic 20.0%
!!! CTTTGTTAACCTAATTTTTA+TGG + chr8_2:3071997-3072016 None:intergenic 20.0%
!! CATAAAAATTAGGTTAACAA+AGG - chr8_2:3071995-3072014 Msa1227710:intron 20.0%
!!! AAAGGTTCAATTAATTTTGA+GGG - chr8_2:3072013-3072032 Msa1227710:intron 20.0%
!! ATTCGTAATTAACAATAAAG+GGG - chr8_2:3072090-3072109 Msa1227710:intron 20.0%
!!! CTCTAAAAGTGTTTTTATTA+TGG + chr8_2:3072430-3072449 None:intergenic 20.0%
!!! CATAATAAAAACACTTTTAG+AGG - chr8_2:3072428-3072447 Msa1227710:CDS 20.0%
!!! GAAATTAAGTTGATTAATGA+TGG - chr8_2:3072776-3072795 Msa1227710:CDS 20.0%
!!! ACCTTAATATTTATTGGTTT+TGG - chr8_2:3072814-3072833 Msa1227710:CDS 20.0%
!! TTTTATGATATGATATTGGG+TGG - chr8_2:3069692-3069711 Msa1227710:intron 25.0%
!! TTTAAAACGTTGATAGACAA+AGG - chr8_2:3069800-3069819 Msa1227710:intron 25.0%
!! ATTCAAAGTGTTCTTAGTAA+CGG - chr8_2:3069823-3069842 Msa1227710:intron 25.0%
!!! ATTTTTTTTTGTGATGTTCG+GGG - chr8_2:3070132-3070151 Msa1227710:intron 25.0%
! TTATTGTTCTGATACTAATG+CGG + chr8_2:3070323-3070342 None:intergenic 25.0%
! CAGAACAATAATAATTTCCA+TGG - chr8_2:3070332-3070351 Msa1227710:intron 25.0%
!! CATATATCCACATTTTCTAT+TGG - chr8_2:3070937-3070956 Msa1227710:intron 25.0%
! AAATATCCCAATAGAAAATG+TGG + chr8_2:3070947-3070966 None:intergenic 25.0%
! AATTTCACTCGTTAGTTAAA+TGG - chr8_2:3071014-3071033 Msa1227710:intron 25.0%
!!! TTTTATTTTATTTGAGGGGA+GGG + chr8_2:3071123-3071142 None:intergenic 25.0%
!!! GGTGTTTTATTTTATTTGAG+GGG + chr8_2:3071127-3071146 None:intergenic 25.0%
!!! GAGGTGTTTTATTTTATTTG+AGG + chr8_2:3071129-3071148 None:intergenic 25.0%
! GAAACTAAAAAAATGCGTAA+TGG - chr8_2:3071222-3071241 Msa1227710:intron 25.0%
!! TTTTCACGTCAAAAATAAGA+AGG - chr8_2:3071270-3071289 Msa1227710:intron 25.0%
! ATGAAATTGTTGCATATAAC+TGG - chr8_2:3071431-3071450 Msa1227710:intron 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG + chr8_2:3071646-3071665 None:intergenic 25.0%
!! TTTTTGCTTATAATAGTGAC+CGG - chr8_2:3071646-3071665 Msa1227710:intron 25.0%
! CAATAAAAAGTTACTACTCT+TGG - chr8_2:3071932-3071951 Msa1227710:intron 25.0%
! TAGACAAACCCATAAAAATT+AGG - chr8_2:3071985-3072004 Msa1227710:intron 25.0%
!! CAAAGGTTCAATTAATTTTG+AGG - chr8_2:3072012-3072031 Msa1227710:intron 25.0%
!!! TCAATTAATTTTGAGGGAAA+AGG - chr8_2:3072019-3072038 Msa1227710:intron 25.0%
! TTCGTAATTAACAATAAAGG+GGG - chr8_2:3072091-3072110 Msa1227710:intron 25.0%
! GATGAATGAATGATAGTTTA+TGG - chr8_2:3072650-3072669 Msa1227710:CDS 25.0%
! TTGTTTATTGACCAAATTGT+TGG - chr8_2:3072696-3072715 Msa1227710:CDS 25.0%
! TATCGAATAAACCAACAATT+TGG + chr8_2:3072710-3072729 None:intergenic 25.0%
! GCCAAAACCAATAAATATTA+AGG + chr8_2:3072818-3072837 None:intergenic 25.0%
!!! TAATATTTATTGGTTTTGGC+AGG - chr8_2:3072818-3072837 Msa1227710:CDS 25.0%
! TTTCTTACATGGATTGATGA+TGG + chr8_2:3068943-3068962 None:intergenic 30.0%
!! AGATTAGAAGAAAGGTACTA+CGG + chr8_2:3068971-3068990 None:intergenic 30.0%
CTAGAAGAAGATTAGAAGAA+AGG + chr8_2:3068979-3068998 None:intergenic 30.0%
AAAGATATTGCAAGCAGTAT+TGG - chr8_2:3069167-3069186 Msa1227710:CDS 30.0%
! TATTAAGCAATCTAGAGTTG+AGG - chr8_2:3069214-3069233 Msa1227710:intron 30.0%
GAAGAAGAAGAATCTAATTC+AGG + chr8_2:3069300-3069319 None:intergenic 30.0%
CATGTTGAGAATTACATGAT+TGG - chr8_2:3069441-3069460 Msa1227710:intron 30.0%
TAAACAATCTTAGTACCACA+GGG + chr8_2:3069784-3069803 None:intergenic 30.0%
TTAAACAATCTTAGTACCAC+AGG + chr8_2:3069785-3069804 None:intergenic 30.0%
! TTCAAAGTGTTCTTAGTAAC+GGG - chr8_2:3069824-3069843 Msa1227710:intron 30.0%
ATTACCTTAAAATAAGGCGT+TGG + chr8_2:3069916-3069935 None:intergenic 30.0%
ACAAAAACTGTTGTACCAAA+AGG + chr8_2:3069987-3070006 None:intergenic 30.0%
GACAATGCATAATTTATGCA+AGG + chr8_2:3070171-3070190 None:intergenic 30.0%
ATATTAGACATTGGATTTGG+AGG + chr8_2:3070459-3070478 None:intergenic 30.0%
GACATATTAGACATTGGATT+TGG + chr8_2:3070462-3070481 None:intergenic 30.0%
!! GTATATTTAATTGACTTGGC+CGG - chr8_2:3070751-3070770 Msa1227710:intron 30.0%
! AATGCTAATGCTTTTGACAA+TGG + chr8_2:3070826-3070845 None:intergenic 30.0%
! TCTAATTTTATTACGGCTAG+TGG - chr8_2:3070860-3070879 Msa1227710:intron 30.0%
ACTAACGAGTGAAATTAACA+AGG + chr8_2:3071010-3071029 None:intergenic 30.0%
TTTCACTAAGTGAAGGAATT+TGG + chr8_2:3071066-3071085 None:intergenic 30.0%
!!! TTTATTTTATTTGAGGGGAG+GGG + chr8_2:3071122-3071141 None:intergenic 30.0%
!!! GTTTTATTTTATTTGAGGGG+AGG + chr8_2:3071124-3071143 None:intergenic 30.0%
ACTAAAAAAATGCGTAATGG+AGG - chr8_2:3071225-3071244 Msa1227710:intron 30.0%
CTTGCTTGTAAAAGAAAGTT+TGG - chr8_2:3071348-3071367 Msa1227710:intron 30.0%
! TTTTGCTTATAATAGTGACC+GGG - chr8_2:3071647-3071666 Msa1227710:intron 30.0%
ATTAATTGAGTACTCGTTCT+TGG - chr8_2:3072046-3072065 Msa1227710:intron 30.0%
TTAATTGAGTACTCGTTCTT+GGG - chr8_2:3072047-3072066 Msa1227710:intron 30.0%
AATTAACAATAAAGGGGGAA+AGG - chr8_2:3072096-3072115 Msa1227710:intron 30.0%
AAGACGAAAAGTAAATGAAG+AGG + chr8_2:3072289-3072308 None:intergenic 30.0%
!! TCTTCATTTACTTTTCGTCT+TGG - chr8_2:3072288-3072307 Msa1227710:CDS 30.0%
TGAACATCTTACATTGGTAT+TGG + chr8_2:3072330-3072349 None:intergenic 30.0%
CATTGATGAACATCTTACAT+TGG + chr8_2:3072336-3072355 None:intergenic 30.0%
! AATGAAGTGTGCTGATAAAA+TGG - chr8_2:3072733-3072752 Msa1227710:CDS 30.0%
TTGATTAATGATGGTTGTGA+AGG - chr8_2:3072785-3072804 Msa1227710:CDS 30.0%
! TGAAATACAAGTCATCGATT+CGG - chr8_2:3072869-3072888 Msa1227710:CDS 30.0%
TATAGAATATGGGGAAATGT+AGG - chr8_2:3072913-3072932 Msa1227710:five_prime_UTR 30.0%
AATCAAGAAATGCAGTACAT+AGG + chr8_2:3073000-3073019 None:intergenic 30.0%
!! AATTTGTTGGCAATGGCATT+AGG + chr8_2:3069042-3069061 None:intergenic 35.0%
AAATGTGTCCGAGAATTTGT+TGG + chr8_2:3069055-3069074 None:intergenic 35.0%
!! TTGATGATGATGAGAGGTAT+GGG - chr8_2:3069343-3069362 Msa1227710:intron 35.0%
GTTGAGAATTACATGATTGG+TGG - chr8_2:3069444-3069463 Msa1227710:intron 35.0%
TGAGTAGTAGAAGTAGAAAG+AGG + chr8_2:3069531-3069550 None:intergenic 35.0%
! TCATCAAGATGCTTGGATTT+TGG - chr8_2:3069548-3069567 Msa1227710:intron 35.0%
! TTCAAGGCATGGATTAATCA+AGG - chr8_2:3069743-3069762 Msa1227710:intron 35.0%
AACACTTGCCAAAATCACTA+CGG - chr8_2:3069859-3069878 Msa1227710:intron 35.0%
! CAAATTGTCCGTAGTGATTT+TGG + chr8_2:3069870-3069889 None:intergenic 35.0%
TTGCATAACCAAGGATTCAT+CGG - chr8_2:3069887-3069906 Msa1227710:intron 35.0%
! ATAATTAGCTAGCGACCTTT+TGG - chr8_2:3069969-3069988 Msa1227710:intron 35.0%
! TATATAATTTTGCACGCGCT+AGG - chr8_2:3070071-3070090 Msa1227710:intron 35.0%
AAAACATGTGGCGTAATTGA+TGG - chr8_2:3070359-3070378 Msa1227710:intron 35.0%
TCCAATGTCTAATATGTCGA+CGG - chr8_2:3070464-3070483 Msa1227710:intron 35.0%
!! TTTGTTGGAAGTTGATACGT+AGG - chr8_2:3070774-3070793 Msa1227710:intron 35.0%
!! TTGTTGGAAGTTGATACGTA+GGG - chr8_2:3070775-3070794 Msa1227710:intron 35.0%
ACAAGACAACATTGCATTGT+TGG + chr8_2:3070902-3070921 None:intergenic 35.0%
AATGTTGTCTTGTCATGAGA+AGG - chr8_2:3070909-3070928 Msa1227710:intron 35.0%
! TAAGTGAAGGAATTTGGAGT+TGG + chr8_2:3071060-3071079 None:intergenic 35.0%
AACTCTCTTTCACTAAGTGA+AGG + chr8_2:3071073-3071092 None:intergenic 35.0%
TACTACTCATATACTCTCTC+CGG - chr8_2:3071515-3071534 Msa1227710:intron 35.0%
TTTGCTTATAATAGTGACCG+GGG - chr8_2:3071648-3071667 Msa1227710:intron 35.0%
GTGCAATATTGCACATGTTA+AGG + chr8_2:3071967-3071986 None:intergenic 35.0%
TATGTATGTTGTCCAAGTCA+AGG - chr8_2:3072227-3072246 Msa1227710:CDS 35.0%
! TTTGCATTGATACCTTGACT+TGG + chr8_2:3072242-3072261 None:intergenic 35.0%
CACAATTAAACATTGCGAGT+TGG + chr8_2:3072479-3072498 None:intergenic 35.0%
! GTGTGCTGATAAAATGGATT+AGG - chr8_2:3072739-3072758 Msa1227710:CDS 35.0%
GAAATACAAAGGACAAAGTG+CGG - chr8_2:3072846-3072865 Msa1227710:CDS 35.0%
! AAGAGGAAGGGTATAGAATA+TGG - chr8_2:3072902-3072921 Msa1227710:five_prime_UTR 35.0%
! AGAGGAAGGGTATAGAATAT+GGG - chr8_2:3072903-3072922 Msa1227710:five_prime_UTR 35.0%
AAATGCAGTACATAGGATTG+GGG + chr8_2:3072993-3073012 None:intergenic 35.0%
GAAATGCAGTACATAGGATT+GGG + chr8_2:3072994-3073013 None:intergenic 35.0%
AGAAATGCAGTACATAGGAT+TGG + chr8_2:3072995-3073014 None:intergenic 35.0%
TTTGTTGAGTACTACACAAG+TGG + chr8_2:3073040-3073059 None:intergenic 35.0%
!!! CTACGGTTGCTTTTCTTACA+TGG + chr8_2:3068954-3068973 None:intergenic 40.0%
TGCCATTGCCAACAAATTCT+CGG - chr8_2:3069044-3069063 Msa1227710:CDS 40.0%
! GATATTGCAAGCAGTATTGG+CGG - chr8_2:3069170-3069189 Msa1227710:CDS 40.0%
!! ATATTGCAAGCAGTATTGGC+GGG - chr8_2:3069171-3069190 Msa1227710:CDS 40.0%
!!! GTTTTTGCTTCTGAGGCTTT+TGG + chr8_2:3069254-3069273 None:intergenic 40.0%
! TGAAGCAGTTTTTGCTTCTG+AGG + chr8_2:3069261-3069280 None:intergenic 40.0%
TTCTTCTTCTTCTTCGTCGT+CGG - chr8_2:3069308-3069327 Msa1227710:intron 40.0%
!! GTTGATGATGATGAGAGGTA+TGG - chr8_2:3069342-3069361 Msa1227710:intron 40.0%
CAAACAATAATGTGCGTCCT+TGG - chr8_2:3069391-3069410 Msa1227710:intron 40.0%
CATGCTTGTAACAACGAAGT+GGG + chr8_2:3069425-3069444 None:intergenic 40.0%
ACATGCTTGTAACAACGAAG+TGG + chr8_2:3069426-3069445 None:intergenic 40.0%
ACACAATTTCATCCACGATG+AGG + chr8_2:3069491-3069510 None:intergenic 40.0%
CTACTACTCATCAAGATGCT+TGG - chr8_2:3069541-3069560 Msa1227710:intron 40.0%
TGAACATCGCCGAATCAAAT+GGG + chr8_2:3069727-3069746 None:intergenic 40.0%
TTGAACATCGCCGAATCAAA+TGG + chr8_2:3069728-3069747 None:intergenic 40.0%
!! ATTTGATTCGGCGATGTTCA+AGG - chr8_2:3069727-3069746 Msa1227710:intron 40.0%
!! TTAGTAACGGGTAGATACGA+AGG - chr8_2:3069836-3069855 Msa1227710:intron 40.0%
ACGGACAATTTGCATAACCA+AGG - chr8_2:3069878-3069897 Msa1227710:intron 40.0%
!!! GCTACCAACGCCTTATTTTA+AGG - chr8_2:3069909-3069928 Msa1227710:intron 40.0%
TTTGTGATGTAAGCCTCGTT+AGG - chr8_2:3070002-3070021 Msa1227710:intron 40.0%
TTGTCATGAAAACGTTCCCT+AGG + chr8_2:3070049-3070068 None:intergenic 40.0%
! TGAGCTTAACTCAGTTGGTA+AGG + chr8_2:3070193-3070212 None:intergenic 40.0%
TACCAACTGAGTTAAGCTCA+CGG - chr8_2:3070193-3070212 Msa1227710:intron 40.0%
TTCCATGGAGACAAAACATG+TGG - chr8_2:3070347-3070366 Msa1227710:intron 40.0%
CCAATGTCTAATATGTCGAC+GGG - chr8_2:3070465-3070484 Msa1227710:intron 40.0%
CGTATCAACTTCCAACAAAC+CGG + chr8_2:3070773-3070792 None:intergenic 40.0%
! TTATTACGGCTAGTGGATAC+AGG - chr8_2:3070867-3070886 Msa1227710:intron 40.0%
AGACAACATTGCATTGTTGG+AGG + chr8_2:3070899-3070918 None:intergenic 40.0%
GCGGAGAAACTTAGAAAATG+TGG - chr8_2:3071198-3071217 Msa1227710:intron 40.0%
TTGCTTATAATAGTGACCGG+GGG - chr8_2:3071649-3071668 Msa1227710:intron 40.0%
GTTCTTTATTCATACTCCCC+CGG + chr8_2:3071668-3071687 None:intergenic 40.0%
GTAAGCAAGCATGCCAAATT+TGG + chr8_2:3072206-3072225 None:intergenic 40.0%
!! ATACAAGTCATCGATTCGGA+AGG - chr8_2:3072873-3072892 Msa1227710:exon 40.0%
! GAGGAAGGGTATAGAATATG+GGG - chr8_2:3072904-3072923 Msa1227710:five_prime_UTR 40.0%
!! GTCCGAGAATTTGTTGGCAA+TGG + chr8_2:3069049-3069068 None:intergenic 45.0%
!! TATTGCAAGCAGTATTGGCG+GGG - chr8_2:3069172-3069191 Msa1227710:CDS 45.0%
! GCGGGGTTAACGTTTTTGTT+GGG - chr8_2:3069189-3069208 Msa1227710:intron 45.0%
! CGGGGTTAACGTTTTTGTTG+GGG - chr8_2:3069190-3069209 Msa1227710:intron 45.0%
!! TGGTGGTTGATGATGATGAG+AGG - chr8_2:3069337-3069356 Msa1227710:intron 45.0%
! AGATGCTTGGATTTTGGACG+TGG - chr8_2:3069554-3069573 Msa1227710:intron 45.0%
CAGATGCGACCCATTTGATT+CGG - chr8_2:3069715-3069734 Msa1227710:intron 45.0%
TTAGTACCACAGGGTTTGCA+GGG + chr8_2:3069775-3069794 None:intergenic 45.0%
GATGTAAGCCTCGTTAGGTT+AGG - chr8_2:3070007-3070026 Msa1227710:intron 45.0%
ACTATCCACCTAACCTAACG+AGG + chr8_2:3070018-3070037 None:intergenic 45.0%
TGGATAGTGACTGCTACCTA+GGG - chr8_2:3070030-3070049 Msa1227710:intron 45.0%
ACCAACTGAGTTAAGCTCAC+GGG - chr8_2:3070194-3070213 Msa1227710:intron 45.0%
AGACATTGGATTTGGAGGAG+TGG + chr8_2:3070454-3070473 None:intergenic 45.0%
CCCGTCGACATATTAGACAT+TGG + chr8_2:3070468-3070487 None:intergenic 45.0%
!! AATTGACTTGGCCGGTTTGT+TGG - chr8_2:3070759-3070778 Msa1227710:intron 45.0%
CTCACTGAGCACTCTTAATG+CGG - chr8_2:3071179-3071198 Msa1227710:intron 45.0%
! TGCGTAATGGAGGAACTTCA+TGG - chr8_2:3071235-3071254 Msa1227710:intron 45.0%
GCTGCTGACAATGCCAAATT+TGG - chr8_2:3072190-3072209 Msa1227710:CDS 45.0%
! GGCAGGAGTGTGAAATACAA+AGG - chr8_2:3072835-3072854 Msa1227710:CDS 45.0%
GATTCGGAAGGAGATTGAAG+AGG - chr8_2:3072885-3072904 Msa1227710:exon 45.0%
GGAAGGAGATTGAAGAGGAA+GGG - chr8_2:3072890-3072909 Msa1227710:five_prime_UTR 45.0%
!!! TTTTATTTATTTTAAAGATT+TGG + chr8_2:3070710-3070729 None:intergenic 5.0%
! GGCGGGGTTAACGTTTTTGT+TGG - chr8_2:3069188-3069207 Msa1227710:intron 50.0%
TTCTTCGTCGTCGGAGATGA+TGG - chr8_2:3069317-3069336 Msa1227710:intron 50.0%
GCATGACATGAACCTCATCG+TGG - chr8_2:3069476-3069495 Msa1227710:intron 50.0%
ATTCGGCGATGTTCAAGGCA+TGG - chr8_2:3069732-3069751 Msa1227710:intron 50.0%
CTTAGTACCACAGGGTTTGC+AGG + chr8_2:3069776-3069795 None:intergenic 50.0%
!! GTTGGTAGCCGATGAATCCT+TGG + chr8_2:3069898-3069917 None:intergenic 50.0%
GTAAGCCTCGTTAGGTTAGG+TGG - chr8_2:3070010-3070029 Msa1227710:intron 50.0%
GTGGATAGTGACTGCTACCT+AGG - chr8_2:3070029-3070048 Msa1227710:intron 50.0%
CCAACTGAGTTAAGCTCACG+GGG - chr8_2:3070195-3070214 Msa1227710:intron 50.0%
AGTTAAGCTCACGGGGACAA+TGG - chr8_2:3070202-3070221 Msa1227710:intron 50.0%
GTTAAGCTCACGGGGACAAT+GGG - chr8_2:3070203-3070222 Msa1227710:intron 50.0%
! CGCCACATGTTTTGTCTCCA+TGG + chr8_2:3070352-3070371 None:intergenic 50.0%
! GGGGAAAGGCACAATAGACT+TGG - chr8_2:3072110-3072129 Msa1227710:intron 50.0%
CGGAAGGAGATTGAAGAGGA+AGG - chr8_2:3072889-3072908 Msa1227710:five_prime_UTR 50.0%
!! ACCAGTGGACTGATCTCCAA+GGG - chr8_2:3072938-3072957 Msa1227710:five_prime_UTR 50.0%
!! TGATCTCCAAGGGGAGTGTT+TGG - chr8_2:3072948-3072967 Msa1227710:five_prime_UTR 50.0%
!! GATCTCCAAGGGGAGTGTTT+GGG - chr8_2:3072949-3072968 Msa1227710:five_prime_UTR 50.0%
! TTCGTCGTCGGAGATGATGG+TGG - chr8_2:3069320-3069339 Msa1227710:intron 55.0%
! ATGAGAGGTATGGGCTGAGC+TGG - chr8_2:3069352-3069371 Msa1227710:intron 55.0%
CGAAGTGGGACTGTCTTCCA+AGG + chr8_2:3069411-3069430 None:intergenic 55.0%
AATGTGCCCTGCAAACCCTG+TGG - chr8_2:3069766-3069785 Msa1227710:intron 55.0%
CCCCGTGAGCTTAACTCAGT+TGG + chr8_2:3070198-3070217 None:intergenic 55.0%
GGGGAAATGTAGGAGACCAG+TGG - chr8_2:3072923-3072942 Msa1227710:five_prime_UTR 55.0%
!! GACCAGTGGACTGATCTCCA+AGG - chr8_2:3072937-3072956 Msa1227710:five_prime_UTR 55.0%
!! CCAGTGGACTGATCTCCAAG+GGG - chr8_2:3072939-3072958 Msa1227710:five_prime_UTR 55.0%
GATTGCCCAAACACTCCCCT+TGG + chr8_2:3072957-3072976 None:intergenic 55.0%
! AGAGGTATGGGCTGAGCTGG+AGG - chr8_2:3069355-3069374 Msa1227710:intron 60.0%
! GTATGGGCTGAGCTGGAGGT+TGG - chr8_2:3069359-3069378 Msa1227710:intron 60.0%
GCTGAGCTGGAGGTTGGCAA+TGG - chr8_2:3069365-3069384 Msa1227710:intron 60.0%
CCCCTTGGAGATCAGTCCAC+TGG + chr8_2:3072942-3072961 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 3068939 3073102 3068939 ID=Msa1227710;Name=Msa1227710
chr8_2 mRNA 3068939 3073102 3068939 ID=Msa1227710-mRNA-1;Parent=Msa1227710;Name=Msa1227710-mRNA-1;_AED=0.12;_eAED=0.12;_QI=214|1|1|1|1|1|3|85|287
chr8_2 exon 3068939 3069201 3068939 ID=Msa1227710-mRNA-1:exon:855;Parent=Msa1227710-mRNA-1
chr8_2 exon 3072111 3072323 3072111 ID=Msa1227710-mRNA-1:exon:854;Parent=Msa1227710-mRNA-1
chr8_2 exon 3072416 3073102 3072416 ID=Msa1227710-mRNA-1:exon:853;Parent=Msa1227710-mRNA-1
chr8_2 five_prime_UTR 3072889 3073102 3072889 ID=Msa1227710-mRNA-1:five_prime_utr;Parent=Msa1227710-mRNA-1
chr8_2 CDS 3072416 3072888 3072416 ID=Msa1227710-mRNA-1:cds;Parent=Msa1227710-mRNA-1
chr8_2 CDS 3072111 3072323 3072111 ID=Msa1227710-mRNA-1:cds;Parent=Msa1227710-mRNA-1
chr8_2 CDS 3069024 3069201 3069024 ID=Msa1227710-mRNA-1:cds;Parent=Msa1227710-mRNA-1
chr8_2 three_prime_UTR 3068939 3069023 3068939 ID=Msa1227710-mRNA-1:three_prime_utr;Parent=Msa1227710-mRNA-1
Gene Sequence

>Msa1227710

ATGATGCTAAAGATAAAGATATTGCAAGCAGTATTGGCGGGGTTAACGTTTTTGTTGGGGTTATTAAGCAATCTAGAGTTGAGGCTGCAGAAACAGAGCCAAAAGCCTCAGAAGCAAAAACTGCTTCAGATGATGAATATCAAACCTGAATTAGATTCTTCTTCTTCTTCGTCGTCGGAGATGATGGTGGTTGATGATGATGAGAGGTATGGGCTGAGCTGGAGGTTGGCAATGGAGACAAACAATAATGTGCGTCCTTGGAAGACAGTCCCACTTCGTTGTTACAAGCATGTTGAGAATTACATGATTGGTGGACAATACGAGCATGACATGAACCTCATCGTGGATGAAATTGTGTTTTATGCATCTCAGATTCCTCTTTCTACTTCTACTACTCATCAAGATGCTTGGATTTTGGACGTGGACGACACTTGTATCTCTAATATTCCTTATTATAAAGCTAAGAGATTTGGATGCGACCCATTTGATTCGGCGATGTTCAAGGCATGGATTAATCAAGGAATGTGCCCTGCAAACCCTGTGGTACTAAGATTGTTTAAAACGTTGATAGACAAAGGATTCAAAGTGTTCTTAGTAACGGGTAGATACGAAGGAACACTTGCCAAAATCACTACGGACAATTTGCATAACCAAGGATTCATCGGCTACCAACGCCTTATTTTAAGGAGTGTGAAATACAAAGGACAAAGTGCGGTGAAATACAAGTCATCGATTCGGAAGGAGATTGAAGAGGAAGGGTATAGAATATGGGGAAATGTAGGAGACCAGTGGACTGATCTCCAAGGGGAGTGTTTGGGCAATCGTACTTTCAAGATCCCCAATCCTATGTACTGCATTTCTTGA
Protein sequence

>Msa1227710

MMLKIKILQAVLAGLTFLLGLLSNLELRLQKQSQKPQKQKLLQMMNIKPELDSSSSSSSEMMVVDDDERYGLSWRLAMETNNNVRPWKTVPLRCYKHVENYMIGGQYEHDMNLIVDEIVFYASQIPLSTSTTHQDAWILDVDDTCISNIPYYKAKRFGCDPFDSAMFKAWINQGMCPANPVVLRLFKTLIDKGFKVFLVTGRYEGTLAKITTDNLHNQGFIGYQRLILRSVKYKGQSAVKYKSSIRKEIEEEGYRIWGNVGDQWTDLQGECLGNRTFKIPNPMYCIS*